Solexa tag analysis

0620

ahsan

今日の結果• 発現量の比較結果

– Upload 済み• 遺伝子の名前で keyword 検索できる• Distance20 かつ tag40 以上 cluster を作

りました。– Cluster の代表 TSS 決まる core 　

promoter 領域の motif 解析– Core promoter motif の　 module の存在– Annotation TSS から代表 TSS の距離– Annotation TSS から cluster 開始点の距離

Tag to Gene Relation

Keyword 検索

URL: http://dev.utgenome.org/~utgb/drosophila/genome_browser/browser.php

Keyword: 　遺伝子名前を記入するKeykinds: 　 geneName を選択する

FBtr0083733 sqz-RAFBtr0084012 ppan-RAFBtr0078728 Xe7-RAFBtr0078729 Xe7-RBFBtr0081642 Aly-RAFBtr0076616 Gug-RA

FlybaseTrID GeneName

DNA Replication Element Binding Factor

Image1 :Core promoter. Transcription Start Site (TSS) position is 0, where TATA at -30 to -25 , DPE at +29 to +33, MTE at downstream. DRE position is not restricted by upstream of TSS.

DRE TATA Inr DPE

Initiator Downstream Promoter Element

TATA Box

MTE

Motif Ten Element

Core promoter Structure

MotifChromosom

alCore

promoter 

DRE 23,792 527  

TATA 31,629 715  

INR 52,111 371  

DPE 29,781 455  

MTE 45,196 547  

motif element in Drosophila Chromosome and RepTSS core promoter

TATA motif position in ResTSS core promoter

INR motif position in ResTSS core promoter B

DPE motif position in ResTSS core promoter C

MTE motif position in ResTSS core promoter D

(TATA,INR,DPE,MTE) RepTSS #

0000 18,600

0001 505

0010 403

0100 320

1000 652

1100 22

1010 21

1001 18

0110 18

0101 10

0011 12

0111 0

1011 1

1110 0

1101 1

1111 0

co-existence of binding motif in core promoter region

Here 0,1 express, the present and absent of each motif elements in written order

TSS distance

Larva(distance from TSS to RepTSS)

0

200

400

600

800

1000

-1500 -1000 -500 0 500 1000 1500

distance

num

ber

of T

rans

crip

t

AllTag

0

1000

2000

3000

4000

5000

6000

-1000 -500 0 500 1000 1500 2000 2500 3000

cluster StartPoint distance from annotationTSSTr

ansc

riptio

n N

umbe

r

Annotation TSS から代表 TSS の距離 Annotation TSS から Cluster の開始点の距離