shandon varistain gemini - thermo fisher scientific a78010402... · xiv 31⁄2” floppy disk drive...

A78010402 Issue 4

OPERATOR GUIDE

A78010402

ENGLISHIssue 4

Shandon Varistain® GeminiAUTOMATIC STAINER

A78010402 Issue 42 A78010402 Issue 4 3

SHANDON VARISTAIN GEMINIOPERATOR GUIDE

LIST OF CONTENTS

WELCOME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 SAFETY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91.1 OVERVIEW . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

INSTALLATION AND SETTING UP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112.1 PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112.2 TO UNPACK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122.3 TO LEVEL THE INSTRUMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132.4 TO FIT THE FILTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142.5 TO CONNECT THE WATER SUPPLY AND DRAINAGE . . . . . . . . . . . . . . . .142.6 TO ADJUST THE TURNTABLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .152.7 TO CONNECT THE SYSTEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .162.8 ELECTRICAL REQUIREMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .182.9 TO SWITCH ON AND OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192.10 ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

CONTROLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253.1 DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253.2 MAIN CONTROL PANEL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253.3 LOAD/UNLOAD CONTROLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28

OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29 4.2 HOW TO STAIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .294.3 HOUSEKEEPING TASKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58

CLEANING AND MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 635.1 CLEANING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .635.2 MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64

TROUBLE SHOOTING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 656.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65TABLE 1 - EQUIPMENT STATUS CHECKS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66TABLE 2 - NUCLEAR STAINING PROBLEMS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67TABLE 3 - CYTOPLASMIC STAINING PROBLEMS . . . . . . . . . . . . . . . . . . . . . . . . .71TABLE 4 - MISCELLANEOUS STAINING PROBLEMS . . . . . . . . . . . . . . . . . . . . . .75TABLE 5 - TROUBLESHOOTING HAEMATOXYLIN STAINING . . . . . . . . . . . . . . . . .78TABLE 6 - TROUBLESHOOTING PAPANICOLAOU STAINING . . . . . . . . . . . . . . . . .79

SPECIFICATION AND ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 807.1 SPECIFICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .807.2 ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82

Thermo Electron Corporation makes every endeavour to ensure that the information contained in its support documentation is correct and clearly stated but does not accept responsibility for any errors or omissions. The development of Thermo products and services is continuous. Make sure that any published information that you use for reference is up to date and relates to the status of the product. If necessary, check with Thermo or your local Thermo representative.

© 2003 Thermo Electron Corporation. All rights reserved.This manual may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to any

electronic or machine readable form without prior written consent of Thermo.

The Shandon Varistain Gemini meets the following CE Mark requirements:

In Vitro Diagnostic Directive 98/79/ECLow Voltage Directive 73/23/EEC, as amended by 93/68/EEC.

SYMBOLS

The following symbols and conventions are used throughout this manual and on the instrument.

THIS SYMBOL WARNS YOU THAT YOU MUST FOLLOW INSTRUCTIONS FOR SAFE AND CORRECT OPERATION. IF THIS SYMBOL APPEARS ON THE INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.

THIS SYMBOL WARNS YOU THAT SURFACES ARE HOT. IF THIS SYMBOL APPEARS ON THE INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.

THIS SYMBOL WARNS YOU THAT THERE MIGHT BE A BIOHAZARD ASSOCIATED WITH THE INSTRUMENT. ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF THE SAMPLES USED. TAKE SUITABLE PRECAUTIONS.

THIS SYMBOL WARNS YOU THAT HARMFUL CHEMICALS ARE USED WITH THE INSTRUMENT. REFER TO THE MATERIAL SAFETY DATA SHEETS FOR THE CHEMICALS USED. ALWAYS ACT WITH COMMON SENSE AND BE AWARE OF LOCAL LABORATORY PROCEDURES. TAKE SUITABLE PRECAUTIONS.

A warning is given in the document if there is a danger of personal injury or damage to samples or equipment.

Note1 Notes give more information about a job or instruction but do not form part of the

instruction

Thermo Shandon Limited is an ISO 9001 and TickIT Accredited CompanyThermo Electron Corporation is the trading name of Thermo Shandon Limited

All information contained in this manual is proprietary and confidential, and the exclusive property of Thermo Electron Corporation. This manual is protected by copyright and reproduction is prohibited. This manual is for use only by the individuals to whom it has been made available by Thermo Electron Corporation.

WARNING

®

C US

A78010402 Issue 44 A78010402 Issue 4 5

WELCOME

1 INTRODUCTION

Welcome to the Shandon Varistain Gemini stainer. Designed and made with care, the instrument is safe to use, simple to operate, and easy to maintain.

This Operator Guide gives instructions for the correct operation and use of both versions of the Shandon Varistain Gemini stainer.

2 SAFETY

THIS PARAGRAPH DETAILS IMPORTANT SAFETY INFORMATION. PLEASE READ THIS PARAGRAPH CAREFULLY.

Thermo products are designed for convenient and reliable service. However, incorrect actions by a user may damage the equipment, or cause a hazard to health. It is important for you to know that:

i The Shandon Varistain Gemini, as supplied, conforms with IEC1010-1. However, the addition of chemicals introduces potential hazards.

As with all scientific equipment, due care and good laboratory practice must be employed when dealing with these chemicals, and consideration must be given to the potential for hazard when dealing with particular chemicals.

Be aware that many of the reagents used with the Shandon Varistain Gemini are flammable. Do not introduce any source of ignition into, or near, the instrument once it has been loaded with reagents.

ii The Shandon Varistain Gemini stainer weighs approximately 80 kilograms (176lbs); get help to move or lift it.

iii Do not remove any panels or covers. The Shandon Varistain Gemini stainer does not have any user serviceable parts inside the instrument or power supply unit.

5

WARRANTY STATEMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85

APPENDIX A TRANSPORTATION INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . 86A.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86A.2 TO REPACKAGE THE STAINER UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86A.3 TO REPACKAGE THE POWER SUPPLY UNIT . . . . . . . . . . . . . . . . . . . . . . .90

APPENDIX B APPROVED REAGENT LIST . . . . . . . . . . . . . . . . . . . . . . . . . . 91B.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91B.2 REAGENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91

APPENDIX C EXAMPLE STAINING PROCEDURES . . . . . . . . . . . . . . . . . . . 92C.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92C.2 HAEMATOXYLIN AND EOSIN STAINING ROUTINE . . . . . . . . . . . . . . . . . .92C.3 PAPANICOLAOU (PAP) STAINING ROUTINE . . . . . . . . . . . . . . . . . . . . . . . .93

APPENDIX D TO PERFORM A MEMORY RESET . . . . . . . . . . . . . . . . . . . . . 95D.1 INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95D.2 INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95

A78010402 Issue 46 A78010402 Issue 4 7

iv This instrument must be properly connected to a good earth (Ground) via the mains input supply.

In compliance with statutory requirements all our equipment is designed to accepted standards of safety. Its use does not entail any hazard if operated in accordance with the instructions given in this manual. However, you must obey the following safety precautions:

i All users must read and understand the Operator Guide, and only operate the unit in accordance with the instructions.

ii Potentially lethal voltages above 110V a.c. or 50V d.c. are present inside the Power Supply Unit (PSU). Do not remove any access covers unless specifically instructed to do so.

iii It is important that normal standards of safety and good laboratory practices are employed. Always use common sense when operating the instrument.

iv Any problems and queries should be referred to your Thermo supplier.

v Correct maintenance procedures are essential for consistent performance. It is recommended that a Maintenance Contract is taken out with our Service Department.

vi Use only factory approved accessories or replacement parts with the stainer.

vii Only use reagents recommended in Appendix B.

viii Disposal of Sealed Lead Acid Battery used in Power Supply Unit (PSU):

The Sealed Lead Acid battery within the Shandon Varistain Gemini Power Supply Unit will need to be replaced every three years.

Notes1 If the Shandon Varistain Gemini has mainly been operated in very low

temperatures, or has been exposed to frequent mains failures, the battery should be replaced every year.

2 The battery manufacturer (Yuasa Battery Sale (UK) Ltd) advise their customers to comply with the relevant regulations within their particular country regarding disposal of this type of battery.

3 The battery used within the Shandon Varistain Gemini Power Supply Unit is :-

NP12-12, 12V, 12Ah, valve regulated sealed lead-acid type rechargeable battery

This battery is classified as, “Class 8, Group III UN No. 2800 Batteries, wet, non-spillable, electric storage, special provision A67”, and meets all requirements of the International Air Transport Association, (I.A.T.A. Dangerous Goods Regulations).

ix Safety and Chemicals

The Shandon Varistain Gemini, as supplied, complies with IEC1010-2-010.

However, chemicals introduce potential hazards and Thermo has adopted the following position with regard to the subject of heated surfaces and volatile chemicals used in medical laboratories:

i Non-specified chemicals are used in the instrument at the customer’s own risk.

ii All the chemicals recommended by Thermo have auto-ignition temperatures considerably above any surface temperature that can be reached during a single fault failure of the heating system on the instrument. Small quantities of paraffin wax present on slides will not reach a temperature that will produce flammable vapour.

iii The instrument contains no source of ignition in any areas of the instrument where chemicals are stored or likely to leak in a single fault condition.

A78010402 Issue 48 A78010402 Issue 4 9

iv The operator is fully aware of the contents of the specification documents detailing the properties of the chemicals they are using.

v The operator has carried out any legally required assessment of chemicals used and is using good laboratory practice.

Attention should be given to the flammability of chemicals used during staining, and sources of ignition should be kept away from the instrument once chemicals have been added.

WARNING

DESCRIPTION

1.1 OVERVIEW

The Shandon Varistain Gemini stainer is a versatile precision engineered bench-top slide stainer, capable of staining large numbers of microscope slides easily and efficiently.

The Shandon Varistain Gemini stainer has been designed for general laboratory use. It can stain multiple batches of 20 slides at any one time using different procedures. It uses two levels for reagent troughs and other facilities - giving a maximum of 26 reagent troughs, six running water positions, and five dry storage or optional heater positions.

The different components of the Shandon Varistain Gemini stainer are shown in Figure 1.1.

a Monitorb Reagent troughs (on both layers)c Load / Unload touch paneld Water wash positionse Air extraction outletf Access door for loading slide basketsg Lower level turntable to aid reagent replenishmenth 31⁄2” floppy disk drivei Main doors for reagent replenishment, cleaning etcj Access door for unloading stained slidesk Main control panell Power Supply Unit (PSU)

a

l k

b

ji

f

d

e

c

gh

Figure 1.1

A78010402 Issue 410 A78010402 Issue 4 11

Special design features of the Shandon Varistain Gemini stainer include:

i A maximum of 26 reagent troughs, 6 running water positions, and 5 dry holding or optional heater positions.

ii Multiple baskets of 20 slides can be processed at the same time

iii Loading and unloading of slide baskets is an easy, one-handed operation

iv Batches of slides are easily transferred to the Shandon Consul automatic coverslipper by the use of the Shandon Consul Clip Transfer Tool

v Heating stations (up to 5) for slide drying (heated units only)

vi Optional agitation of slides

vii Running water wash with automatic flow regulation

viii Multiple procedures can be used at any one time

ix Ability to assign a run as urgent or routine, or to cancel a procedure and retrieve slides

x Visible and audible indication of completion of procedure

xi Use with a standard PC compatible colour monitor

xii Battery back-up allows the instrument to be used for up to 40 minutes after mains power failure to complete staining

xiii Parallel Centronics printer output is available to provide a record of runs and to assist in reagent management

xiv 31⁄2” floppy disk drive allows for easy software update and data exchange

Routine maintenance of the Shandon Varistain Gemini stainer requires only that the charcoal filter is regularly changed and that the instrument is kept clean.

INSTALLATION AND SETTING UP

2.1 PROCEDURE

The Shandon Varistain Gemini stainer is a bench mounted instrument that must be unpacked and installed with care.

The Shandon Varistain Gemini stainer must be positioned within 1.8m (6 ft) of a drain and water supply.

The bench should be rigid and level, and made of a non-flammable material. It must be at least 600mm (24ins) deep and be large enough for the Power Supply Unit and Monitor to be sited near to the Stainer. It must be able to hold their combined weight (Stainer: 80kg (176lbs; Power Supply Unit: 11kg (24lbs); Monitor: 20kg (44lbs)).

Make sure that there is at least 100mm (4”) clearance around the fan inlet on the left hand side of the instrument.

The maximum overall dimensions of the Shandon Varistain Gemini stainer are:

Width 720mm (281⁄2ins)

Depth 710mm (28ins)

Height 880mm (341⁄2ins)

Weight 80kg (176lbs)

The Stainer weighs approximately 80 kilograms (176lbs). Get help to safely move or lift the instrument without risk of injury.

>100mm

>100mm

��

�

� � � � ��

��

� �

��

��

��

��

<1.8m

WARNING

A78010402 Issue 412 A78010402 Issue 4 13

The Stainer weighs approximately 80 kilograms (176lbs). Get help to safely move or lift the instrument without risk of injury.

Remove the packaging that was used to secure the reagent troughs, baskets and arm from inside the stainer unit.

Note1 The large evaporation lids are packed inside the instrument

Make sure that you have received all the parts listed on the packing list supplied with the instrument. Contact your Thermo dealer if necessary.

Notes1 Inform your Thermo dealer immediately if there are any breakages or shortages.

Quote the instrument Serial Number, your Order Number, Invoice Number, Delivery Note (or Packing Slip) Number and the date.

2 For optimum performance of a heated Shandon Varistain Gemini, make sure that there is at least 100mm (4”) clearance around the fan inlet on the left hand side of the instrument.

3 If you ever need to transport the instrument, refer to Appendix A for instructions about how to repack the instrument.

2.3 TO LEVEL THE INSTRUMENT

The Shandon Varistain Gemini stainer must be level before it is used for the first time. It is fitted with levelling feet that may need to be adjusted to suit its working location.

To adjust the levelling feet, first use a spanner to loosen the lock nut (a).

Adjust the height of the foot with a spanner on the lower nut (b). When the height is correct, tighten the lock nut (a).

The maximum overall dimensions of the associated Power Supply Unit are:

Width 360mm (14ins)

Depth 463mm (181⁄4ins)

Height 145mm (61⁄2ins)

Weight 11kg (24lbs)

2.2 TO UNPACK

If the packaging has been damaged, check the condition of the instrument. Contact your Thermo dealer if there is any damage.

Remove the top layer of packaging from the Shandon Varistain Gemini Power Supply Unit. Get help if necessary to lift the instrument from the box and to place it on the bench.

To lift or move the Shandon Varistain Gemini Power Supply Unit, hold the box securely under the sides (a) of the unit.

Get help if needed to remove the monitor (maximum allowable monitor weight 30kg (66lbs)) from its packing and place on top of the Power Supply Unit (b) (follow the manufacturer’s instructions).

Remove the top layer of packaging from the Shandon Varistain Gemini stainer. Get help to lift the instrument from the box and to place it on the bench.

To lift or move the Shandon Varistain Gemini stainer, hold the instrument securely under the sides (c).

��

�

� � � � ��

��

� �

��

b

c c

WARNING

aa

a

b

A78010402 Issue 414 A78010402 Issue 4 15

Water pressure must be between a minimum of 0.2bar (3psi) and a maximum of 10bar (145psi). If the water pressure is greater than 10bar (145psi), install a pressure reducing valve.

Note1 A flow regulator is fitted to the

instrument to automatically regulate the water flow.

2.5.2 To Connect the Drainage

The drain hose is already fitted to the instrument (e). It should be run across the back of the instrument to the drain point (f).

Make sure that the drain hose is laid flat across the bench.

Notes1 Shorten the drain hose as required to keep it as short as possible.

2 To clean the inside of the drain hose, flush through with 10% commercial bleach.

2.6 TO ADJUST THE TURNTABLE

The Turntable on the lower level of the Shandon Varistain Gemini stainer can be moved to facilitate cleaning and loading of reagents. It must be in position and locked before starting to stain.

To adjust the Turntable, open the Main Access Doors. Lift the two black Locking Knobs (a) on the Turntable until they click into the raised position.

2.4 TO FIT THE FILTER

A CARBON FILTER MUST BE INSTALLED IN THE SHANDON VARISTAIN GEMINI STAINER AT ALL TIMES. IF NO FILTER IS FITTED, THE EXTRACTOR FAN CANNOT BE OPERATED.

Note1 An optional vent kit is available to

allow any fumes to be extracted into a fume cupboard or vented to the outside atmosphere.

Remove the filter from its packaging. Remove the cellophane wrapping and place the filter into the front recess of the top cover channel (a). Make sure that the ‘air flow’ arrow is visible from the front of the unit and that it is pointing upwards into the cover.

Slide the filter along the channel towards the rear of the unit. Make sure that it slides over the spring flaps, and that it is secure against the rear of the channel (b).

2.5 TO CONNECT THE WATER SUPPLY AND DRAINAGE

2.5.1 To Connect the Water Supply

A 2.5m (8 ft) supply hose is supplied with the instrument. This must be fitted before use.

To fit the supply hose, screw the end with the 90° elbow to the threaded fitting on the back of the instrument at the left hand side (c) (looking at the instrument from the rear). Secure the other end to a water supply (d).

a

b

WARNING

c

d

ef

WARNING

a

A78010402 Issue 416 A78010402 Issue 4 17

Note1 If the monitor’s data cable is too short to reach the stainer, use the extension

lead that is supplied with the Shandon Varistain Gemini stainer.

2.7.2 To Connect the Stainer to the Power Supply Unit

Connect the 25-way data cable to the middle connector on the Stainer (a) and the 25-way connector on the rear of the Power Supply Unit marked ‘IOIOI’ (c).

Note

1 The right hand connector (b) on the stainer (i.e. the connector nearest to the front of the instrument) is for service engineer use only.

2.7.3 To Connect a Printer

Any dot matrix or inkjet printer that typically connects to the LPT1 Centronix printer port of an IBM* compatible computer is suitable for use with the Shandon Varistain Gemini stainer.

The printer connects only to the Power Supply Unit and produces a hard copy record of the activity of any procedure on demand.

To connect a printer, use a properly screened and terminated data input cable that does not exceed 3m in length. The cable connects from the right hand connector on the rear of the Power Supply Unit (d) (marked with a printer symbol) to the printer.

* IBM is a registered mark of International Business Machines.

Gently rotate the Turntable to load reagent troughs or to clean the instrument. (Refer to Section 4.2.8 for instructions about how to load reagents into the instrument).

Note1 Do not turn the Turntable quickly if reagents are loaded in the instrument -

reagents are likely to spill.

To lock the Turntable, gently rotate the Turntable until positions C and D are in the centre of the front of the instrument. Push the two Locking Knobs down and gently move the Turntable a small distance from side to side until the Locking Knobs locate into their locking positions.

Check that the Locking Knobs are both locked in the lowered position.

Note1 The Main Access Doors will not close if the Turntable is not in the correct

position, or if either Locking Knob is raised.

2.7 TO CONNECT THE SYSTEM

Make sure the power is switched off before connecting any cables, otherwise damage can occur.

2.7.1 To Connect the Monitor to the Stainer

Consult the monitor’s instructions to identify the data cable. Connect the monitor’s data cable to the left hand connector on the Stainer (a) (i.e. the connector nearest to the rear of the instrument marked with a monitor symbol).

Follow the manufacturer’s instructions to connect the monitor’s power supply lead to the mains power supply.

a

WARNING

Product:Cat No:Serial No:

Nominal Ratings:

��

A78010351 Issue 10PS0001E0001

��

��

�

� ��

cd

a b

A78010402 Issue 418 A78010402 Issue 4 19

2.8 ELECTRICAL REQUIREMENTS

THE STAINER IS LOW VOLTAGE (40V D.C.). IT MUST ALWAYS BE POWERED BY THE SHANDON VARISTAIN GEMINI POWER SUPPLY UNIT.

Make sure that the voltage of the mains supply corresponds with the voltage rating on the rating plate on the back of the Power Supply Unit.

Note

1 The ~ symbol on the rating plate indicates that the instrument operates on an alternating current supply (a.c.)

Make sure that the I / O power switch at the rear of the instrument is switched OFF (O side of the switch pushed inward) (a).

Instruments are supplied with power cords with moulded plugs suitable for the UK, France and Germany or North America. If another plug is required, it is necessary for a technically competent person to remove the moulded plug and fit a suitably rated, fused plug using the wiring convention shown opposite.

Insert the appropriate mains cable into the mains connector on the rear panel of the Power Supply Unit (b) and clip the cable restraint over the mains connector. Connect the mains supply cable to the local power supply outlet.

THE SHANDON VARISTAIN GEMINI STAINER POWER SUPPLY MUST BE PROTECTIVELY EARTHED. MAKE SURE THAT IT IS PLUGGED INTO A PROPERLY EARTHED MAINS SUPPLY.

IT MUST BE POSSIBLE TO INTERRUPT THE MAINS SUPPLY AT SOURCE BY REMOVING THE PLUG FROM THE MAINS SUPPLY SOCKET.

a

Product:Cat No:Serial No:Nominal Ratings:

�� A78010351 Issue 10PS0001E0001��

��

�

� ��

During shipping the Battery Isolation Switch (c) will be switched to the OFF position, (O on Battery Isolation Switch pushed inward).

Once the Power Supply Unit has been connected to both the Stainer and the mains supply, press the I (ON) side of the Battery Isolation Switch (d).

Notes

1 The Battery Isolation Switch should be left in the ON position until the 12V battery is checked at the annual service, or if the PSU is to be transported.

2 Make sure that the correct sequence is followed when the instrument is switched off (see Appendix A).

If the correct sequence is not carried out, the instrument may not operate correctly when it is switched on again. If this happens, follow the instructions in Appendix D to perform a memory reset.

2.9 TO SWITCH ON AND OFF

THE POWER SWITCH ON THE MAINS INLET SOCKET SHOULD ALWAYS BE USED TO TURN THE POWER SUPPLY UNIT ON AND OFF.

Brown wire - Live (L or L2) terminal

Blue wire - Neutral (N or L1) terminal

Green / yellow wire - Earth terminal - E, ground or


Nominal Ratings:

��

A78010351 Issue 10PS0001E0001

��

��

�

� ��

b c

l0

l0

d

A78010402 Issue 420 A78010402 Issue 4 21

IF THE BATTERY ISOLATION SWITCH IS USED TO SWITCH OFF THE INSTRUMENT, ALL OPERATIONAL STATUS INFORMATION WILL BE LOST.

ALL BATCHES IN PROGRESS MUST BE REMOVED FROM THE INSTRUMENT BEFORE THE INSTRUMENT IS SWITCHED ON AGAIN.

2.9.1 To Switch On

Press the I (ON) side of the I / O power switch to switch the instrument on (a).

Initial Battery Charging - after connecting the Power Supply Unit to the Stainer and switching on, it should be left for a period of 14 hours (overnight), to ensure the battery is fully charged.

Follow the instructions on the screen to select the required language.

For example, press 1 for English (b) and then press the left hand function button (c) on the Main Control Panel that corresponds to [SELECT].

2.9.2 To Switch Off

When the instrument is regularly used, the Standby button [ ] (d) on the Main

Control Panel should be used (see Section 3.2.10).

c

b

WELCOME TO VARISTAIN GEMINI STAINER

Press 1 for English Appuyer 2 sur Francais Drucken sie 3 fur Deutsch

Please press SELECT once you have entered the required language

If help is required at any point press the i buttonUse the EXIT key to return from most screens to the previous menu

Welcome 1/1/2000 10:42

Select

Varistain Gemini

a

d

e

If the instrument is to be left unattended for long periods of time, or is to be moved, the power to the instrument should be turned off. Press the O (OFF) side of the power switch to switch off the Shandon Varistain Gemini (e).

THE SHANDON VARISTAIN GEMINI HAS A BATTERY BACK UP IN CASE OF POWER FAILURE. BE AWARE THAT THE ARM MAY MOVE EVEN IF THE MAINS SWITCH IS SWITCHED OFF.

2.10 ACCESSORIES

2.10.1 Shandon Consul Clip Transfer Tool

To load the Shandon Consul Clip Transfer Tool, place the Transfer Tool on a flat surface facing upwards and with the handles fully extended (a).

Insert a 20 Clip Rod into the Transfer Tool with the open end of the Slide Clips facing upwards (b). The Rod locates into the cut out in the end of the Transfer Tool (c).

Note:1 The rod will only fit in the Tool this way

round.

Push the 20 Clip Rod down firmly into the Transfer Tool (d).

Squeeze the handles together (e) and invert the Transfer Tool over the basket of Microscope Slides (f).

Line up the Slides with the Clips for correct orientation into the Consul Coverslipper.

Note:1 Make sure the sample side of the Slides (g) face away from the protruding rod.

Keep the handles squeezed together. Push the Transfer Tool down (h) until the Slides are fully located in the clips.

a

d

c

b

h

g

e

f

A78010402 Issue 422 A78010402 Issue 4 23

Release and fully open the handles of the Transfer Tool (i).

Make sure that no pressure is applied to the handles when the Transfer Tool is removed from the slides (j).

Hold the 20 Clip Rod (k) and pull it upwards to remove the slides from the Basket (l).

The 20 Clip Rod can now be placed on the Consul for coverslipping.

To fit the 20 clip adaptor collar, fit 20 Slide Clips (m) on the standard 40 Clip Rod (n). Make sure the clips are oriented in the correct way.

Note:1 The samples on the slides should face away from

the Adaptor Collars.

Push the first Adaptor Collar (o) over the Rod (with the small diameter first) until it is tight against the clips. Use the Allen Key provided (1.5mm A/F) to tighten the grub screws (q) to secure the collar.

Fit the second collar (p) at the end of the Rod so that the end of the collar sits between the two grooves. Use the Allen Key provided (1.5mm A/F) to tighten the grub screws (q) to secure the collar.

To remove the 20 clip adaptor collar, loosen the grub screws (q), and slide both collars off the rod.

2.10.2 Vent Kit

The Vent Kit should not be used to extract the fumes through the building HVAC system or a common site extraction system.

The optional vent kit allows any fumes to be extracted into a fume cupboard or hood, or vented to the outside atmosphere. Maximum airflow is 58m3 per hour.

The flexible duct (a) should be fixed to the mounting bracket using the large clip provided (b).

Fix the mounting bracket to the top cover over the fan outlet grille (c). Use the 4 screws and gasket provided.

Remove the filter if fitted from inside the top cover.

To fit the fan guard provided (d) on the inlet side of the extraction fan inside the instrument, locate the 4 plastic lugs on the fan guard (e) in the 4 corner holes of the fan

and push firmly into place.

Use the rectangular block to activate the filter sensor. Fit the block in the left hand filter channel, on the underside of the top cover (f).

WARNING

a

bc

i

ji

k

l

q

m

o n

p

d

e

f

A78010402 Issue 424 A78010402 Issue 4 25

Orientate the block so that if fits in the channel and is retained by the filter location spring. Push the block firmly to the back of the channel to activate the sensor (g).

The filter life warning in the user interface must be switched off. The filter life set-up option is within the Quality Control menu. Change the Filter Replacement Warning Interval to ‘Off’.

Note

1 The flexible duct can be cut to length as required.

2.10.3 Large Slide Basket Adaptor

The Large Slide Basket Adaptor has been designed to allow large slides to be used in the Shandon Varistain Gemini slide baskets (up to 75 x 58mm (3” x 21⁄4”)).

Slide the adaptors into the slots in the basket as shown (a), and insert up to four 75 x 58mm (3” x 21⁄4”) slides in the slots in the adaptor.

Hint1 If required, the adaptors can be fitted in

different positions to allow multiple sized slides to be used

CONTROLS3.1 DESCRIPTION

The Shandon Varistain Gemini stainer is a versatile precision engineered automatic stainer. A separate Power Supply Unit (PSU) and monitor are sited beside the stainer on the bench.

The Shandon Varistain Gemini stainer has been designed for general laboratory use. It can process multiple batches of 20 slides using different procedures.

This chapter describes the functions of all the controls on the instrument.

3.2 MAIN CONTROL PANEL

The Main Control Panel is situated on the Power Supply Unit (PSU).

3.2.1 Function Buttons

The function of each of the four blank buttons at the top of the Main Control Panel (a) corresponds to the specific function of the buttons shown at the bottom of each display screen on the colour monitor.

a

��

��

��

��

�

� � � � ��

��

� �

��

a a

g

A78010402 Issue 426 A78010402 Issue 4 27

3.2.4 Cancel

Press [CANCEL] to cancel any selection.

3.2.5 Direction Arrow Keys

Press [ ], [ ], [ ] and [ ] to move the cursor around the screen.

3.2.6 Exit

Press [EXIT] to go back to the previous screen.

3.2.7 [ ]

This key is currently not used, but is reserved for future features.

3.2.8 Silence Alarm [ ]

Press [ ] to silence the alarm. For some alarms, this will

cancel the alarm as well. Other alarms will require separate

actions and acknowledgements.

3.2.9 Help Key [ ]

Press [ ] to display a help screen at any time.

3.2.10 Standby Key [ ]

Press [ ] to switch the instrument into standby mode. The

red LED flashes when Standby is selected. If [ ] is pressed again, the instrument will restart.

An example is shown here:

To choose [STAIN], press the left hand function button (a) as it corresponds to the one shown on the display. The instruction given to press this function button will be “Press [STAIN]”.

Press function button (b) to choose [PROGRAM]. The instruction given to press this function button will be “Press [PROGRAM]”.

Press function button (c) to choose [MONITORING]. The instruction given to press this function button will be “Press [MONITORING]”.

Press function button (d) to choose [OPTIONS]. The instruction given to press this function button will be “Press [OPTIONS]”.

Note

1 This convention for pressing the function keys will be used throughout this Operator Guide.

3.2.2 Number Keys

The number keys are used to input numerical information for use in staining procedures and to select different programs.

3.2.3 Enter

Press [ENTER] if required to confirm any selection.

a dcb

Main Menu 1/1/2000 10:42

Please select the required action

Press STAIN for information on using the Varistain Gemini to stain slidesPress PROGRAM to define or view reagents, procedures and reagent layoutPress MONITORING to view reagent usage and batch progressPress OPTIONS to set up the Varistain Gemini

If help is required at any time press the i buttonUse the EXIT key to return from most screens to the previous menu

Stain Program Monitoring Options

Varistain Gemini

A78010402 Issue 428 A78010402 Issue 4 29

3.3 LOAD/UNLOAD CONTROLS

The Load / Unload Touch Panel is located on the front of the Stainer.

3.3.1 Load Buttons [A] and [B]

These buttons can be used to start the staining of a batch of slides once they have been loaded.

If the LED is lit, the relevant load door is available for a basket of slides to be loaded.

A flashing LED on Load Buttons [ A ] and [ B ] indicates that a load door has been opened, but has not been acknowledged. Press the button to confirm that slides have been loaded.

If the LED is off, the load door is not available.

3.3.2 Unload Buttons [C] and [D]

The LED on each Unload Button flashes when the basket is in position to be unloaded.

Press Unload Buttons [C] and [D] to acknowledge the removal of a batch of slides after staining.

If the LED is off, there are no baskets to be unloaded. Do not open the load door if the LED is off.

3.3.3 Auto Return

Press [AUTO RETURN] to request the return of the last batch of slides loaded. The slides will be returned to the input door where they were loaded to allow the operator to remove them.

��

��

��

��

�

� � � � ��

��

� �

��

OPERATION4.1 INTRODUCTION

It is recommended that the instrument is

kept in Standby mode when it is not in use.

To enter the Standby mode, press the [ ]

button (a).

Note

1 The red LED flashes when the instrument is in STANDBY mode (b)

To restart the instrument, press the [ ] button again.

4.2 HOW TO STAIN

Follow these simple steps to prepare the Shandon Varistain Gemini stainer for staining slides:

1 Select the STAIN option from the Main Menu (paragraph 4.2.1).

2 Define any special reagents to be used (paragraph 4.2.2).

3 Define the staining procedures to be used (paragraph 4.2.3).

4 Use the ‘Reagent Layout’ facility to assign the most efficient use of reagents (paragraph 4.2.5).

5 Load the reagents in their optimal positions (paragraph 4.2.8).

6 Stain the slides (paragraph 4.2.9).

b

��

��

��

��

�

� � � � ��

��

� �

��

a

Main Menu 1/1/2000 10:42





Varistain Gemini

ab

A78010402 Issue 430 A78010402 Issue 4 31

Notes

1 To access help screens at any time, press [ ] (a) on the Main Control Panel.

2 To exit from most screens, press [EXIT] (b) to return to the previous screen.

4.2.1 Select the Stain Program

Once the required language has been selected, the Main Menu will be displayed on the monitor.

Press [STAIN] to enter the STAIN program (i.e. press the left hand function button on the Main Control Panel that corresponds to [STAIN] on the monitor) .

4.2.2 Define the Reagents to Be Used

If a special reagent is used for a particular procedure, it may be necessary to enter the name of the reagent before any slides can be stained.

The ‘Define Reagent’ screen lists 42 commonly used stains and reagents. These cannot be changed, but a further 12 reagents can be added to the list if necessary in the User Defined Reagents section (reagent numbers 43 - 54 - shaded in the illustration).

Varistain GeminiDefine Reagent Names 1/1/2000 10:42

Use the cursor to select a name then press ENTER1 Haematoxylin2 Harris Unacidified3 Harris Acidified4 Gill 15 Gill 26 Gill 37 Aqueous Eosin8 Alcoholic Eosin9 Aqueous Eosin Y10 Alcoholic Eosin Y11 OG-612 EA-3613 EA-5014 EA-6515 Xylene16 Xylene Substitute17 Alcohol18 IMS19 Ethanol20 Isopropyl Alcohol21 Buffer22 Tap Water23 Distilled Water24 Deionised Water25 Acid Alcohol26 Acid Water27 Bluing Reagent28 Ammonia Water29 Periodic Acid Soln30 Schiffs Reagent31 Sulphite Rinse32 Van Gieson Stain

33 Alcian Blue34 Perls Reagent35 Leishman Stain36 May Grunwald Stain37 Giemsa Stain38 Wright Stain39 Jenner Stain40 Dry Storage41 Heater Station42 Running Water Wash

User Defined Reagents

434445464748495051525354

Notes

1 The Heater Stations (reagent 41) are only fitted onto the heated version of the Shandon Varistain Gemini stainer. They are intended to dry slides that are wet with water only.

2 If any of the heater positions are to be used for the dry storage of baskets, then it should be noted that ambient air is blown through the trough when any of the other heaters are on.

3 The Heater Station troughs are held in position by spring clips. To remove them, pull upwards firmly. To refit them, push them down firmly so that the spring clip holds the trough securely.

DO NOT FILL HEATER STATION TROUGHS WITH REAGENTS.

DO NOT SPLASH OR POUR REAGENTS NEAR TO THE HEATER STATION TROUGHS.

To enter a special reagent, press [DEFINE REAGENTS] (a) from the ‘How To Stain’ screen.

Use the direction arrow keys, [ ] and [ ] to move the cursor to the next free number.

When the correct line is highlighted, press [ENTER] on the Main Control Panel. A keyboard will be displayed on the screen. Use the direction arrow keys to select each letter of the new reagent name in turn. Press [ENTER] when the correct letter has been selected.

a

Varistain GeminiHow to Stain 1/1/2000 10:42

Quick Reference for using the Varistain Gemini stainer

1. Use DEFINE REAGENTS to enter the names of reagents to be used

2. Use DEFINE PROTOCOLS to enter the parameters for each program step

3. Use REAGENT Layout to select the procedures currently required

4. Use LOAD REAGENTS to confirm each reagent has been placed in position

5. To stain a batch of slides open the Load Door and follow the on screen instructions for selecting the procedure required

6. When a batch has completed its staining protocol it will be placed behind an unload station door and its acknowledge button light will flash. Open the door and follow the instructions on the screen.

Define Reagents Define Protocols Reagent Layout Load Reagents

Varistain GeminiDefine Reagent Names 1/1/2000 10:42

Use the cursor to select a name then press ENTER1 Haematoxylin2 Harris Unacidified3 Harris Acidified4 Gill 15 Gill 26 Gill 37 Aqueous Eosin8 Alcoholic Eosin9 Aqueous Eosin Y10 Alcoholic Eosin Y11 OG-612 EA-3613 EA-5014 EA-6515 Xylene16 Xylene Substitute17 Alcohol18 IMS19 Ethanol20 Isopropyl Alcohol21 Buffer22 Tap Water23 Distilled Water24 Deionised Water25 Acid Alcohol26 Acid Water27 Bluing Reagent28 Ammonia Water29 Periodic Acid Soln30 Schiffs Reagent31 Sulphite Rinse32 Van Gieson Stain

33 Alcian Blue34 Perls Reagent35 Leishman Stain36 May Grunwald Stain37 Giemsa Stain38 Wright Stain39 Jenner Stain40 Dry Storage41 Heater Station42 Running Water Wash

User Defined Reagents

434445464748495051525354

Main Menu 1/1/2000 10:42





Varistain Gemini

A78010402 Issue 432 A78010402 Issue 4 33

When the name has been fully typed in, press [COMPLETE] (b). This will return you to the ‘Define Reagent Names’ screen.

When all the required User Defined Reagents have been entered, press [EXIT] to return to the ‘How To Stain’ screen.

Notes

1 The User Defined Reagent names can be up to 22 characters long.

2 To delete the last letter entered, press [CANCEL] on the Main Control Panel (c).

3 To switch between capital and lower case letters, press [CAPS LOCK] (d).

4 For other characters e.g. letters with accents etc, press [CONTROL] (e). (To exit from the Control Characters Keyboard, press [CONTROL] again).

4.2.3 Define the Staining Procedures to Be Used

This step will define the staining procedures.

Press [DEFINE PROTOCOLS] in the ‘How To Stain’ screen to enter the ‘Procedure Select’ screen.

Note1 A procedure can be viewed and the times can be edited while it is active. Steps

cannot be inserted or deleted, or the reagents changed. To fully edit an active procedure, it must first be removed from the active list. If an active procedure is selected, a warning box will be displayed.

Caps Lock CompleteControl

1 432 76 #-098 =5

Y [POIUTREWQ ]

‘;LKJHGFDSA

/.,MNBVCXZ\

b

Varistain GeminiKeyboard 1/1/2000 10:42

The text going in

c

d e

Caps Lock CompleteControl

1 432 76 #-098 =5

y {poiutrewq }

@:lkjhgfdsa

?><mnbvcxz|


The text going in

2 To keep the procedure on the active list, press [RETAIN]. This will allow the times in the procedure to be edited.

3 To remove the procedure from the active list, press [REMOVE]. This will remove the procedure from the active list.

4 Once the procedure is removed from the active list, it can be edited as normal.

5 To reassign the procedure to the active list, enter the Reagent Layout 1 screen and reselect the procedure (see paragraphs 4.2.5 and 4.2.6).

There is space for 50 procedures. Use the direction arrows [ ] or [ ] to move to the required procedure, and press [SELECT]. If the procedure chosen is an existing one, the ‘Define Procedure’ screen will be displayed.

If no procedures have been entered, or if you want to enter a new procedure, select an unused number. The ‘Select name’ screen will be displayed.

This screen is used to name a procedure from the seven pre-defined names or by a user defined name. Use the direction arrows to select a name, or to choose an unused number in the user-defined name section. Press [SELECT].

Varistain GeminiProcedure Select 1/1/2000 10:42

Use the cursor to select the procedure required and then press SELECT

No. Procedure Name Notes Duration

1 PAP GYN 24:352 PAP Non GYN 25:2534 H&E Routine 34:3056789101112

Select Change Name Change Notes

Procedure Select 1/1/2000 10:42

Varistain Gemini

Use the cursor to select the procedure required and then press SELECT

No. Procedure Name Notes Duration

101112

Changing the number of steps in a program that is currently selected for use, or altering the reagent, might make the reagent layout invalid.

Select RETAIN to keep this program in use or REMOVE to remove the program from the reagent layout and allow the program to be edited

Retain Remove

A78010402 Issue 434 A78010402 Issue 4 35

If a user defined name was chosen, the keyboard will appear.

Use the direction arrow keys to select each letter of the new procedure name in turn. Press [ENTER] when the correct letter has been selected.

When the name has been fully typed in, press [COMPLETE]. Another keyboard will now be displayed to allow you to enter or change the procedure notes.

Use the direction arrows to choose each letter in turn. Press [ENTER] when the correct letter is selected. When the notes have been entered, press [COMPLETE]. The ‘Define Procedure’ screen will now be displayed.

Notes1 The User Defined Procedure

names can be up to 9 characters long using capital and lower case letters, ‘&’ and ‘.’ only.

2 Notes can be entered to identify any special instructions or information that may be helpful during the set up or running of any procedure.

3 The procedure names and notes can be altered at any time by pressing [CHANGE NAME] or [CHANGE NOTES] in the ‘Procedure Select’ screen.

4 Press [ ] at any time to display the help screen.

Select Name 1/1/2000 10:42

Varistain Gemini

Select a name for the procedure from the list and press SELECT or ENTER

Select

Pre-defined namesPAPH&EPASV. GIESONGIEMSATRICHROMEZN (TB)

User Defined Names1234567891011121314

! $£“ &^ ~_)(* +%

å ïèëêçàäâéü î

ÖÿùûòöôÉÅÄì

²¡¿Ññúóíá¢Ü


The text going in

Caps Lock Control Complete

1 432 76 #-098 =5

Y [POIUTREWQ ]

‘;LKJHGFDSA

/.,MNBVCXZ\


The text going in

Change Notes

Caps Lock Control Complete

4.2.4 Define Procedure Screen

This screen is used to specify the details of each step to be used in a staining procedure.

The name of the procedure chosen and any notes that have been entered are displayed on the top line of the screen.

Each procedure can have up to 50 steps. They are organised into five colour coded sections:

1 Load Purple (1 step only)

2 Pre-Stain Grey

3 Stain Brown

4 Post-Stain Green

5 Unload Red (1 step only)

Notes

1 [INSERT STEP] allows a step to be inserted below the cursor position

2 [DELETE STEP] deletes the selected step

3 [COPY] copies steps or sections from other procedures (or complete procedures) for insertion into another procedure - for example standard steps used for pre- or post-stain

Use the direction arrows [ ] and [ ] to move the cursor

Hint

1 To delete an entire procedure, use the copy facility to copy a blank procedure over the one to be deleted.

Varistain GeminiDefine Procedure 1/1/2000 10:42

Insert Step Delete Step Copy Change Reagent

Procedure no. 2 - H & E - Routine for Dr. xxxxxxx

Step Reagent Conc.% Set Time / Limit Agitate Uses.1 Dry Storage 00:00 No Maximum None2 Xylene 5 01:00 No Maximum None3 Xylene 5 01:00 No Maximum None4 Xylene 5 01:00 No Maximum None5 Alcohol 100 5 01:00 No Maximum None6 Alcohol 100 5 01:00 No Maximum None7 Alcohol 100 5 01:00 No Maximum None8 Running Water - 5 01:00 No Maximum None9 Haematoxylin - 5 04:00 Standard None10 Running Water - 5 01:00 Standard None11 Acid Alcohol - 5 00:15 Standard None12 Running Water - 5 01:00 Standard None13 Aqueous Eosin - 5 02:00 Standard None14 Running Water - 5 01:00 Standard None15 Alcohol 100 5 01:00 No Maximum None16 Alcohol 100 5 01:00 No Maximum None17 Xylene 5 01:00 No Maximum None18 Xylene 5 01:00 No Maximum None19 Xylene 5 00:00 No Maximum None

A78010402 Issue 436 A78010402 Issue 4 37

Each step requires the following information (information marked * must be given):

a * Reagent

b Concentration

c Set Uses

d * Time

e Limit

f Agitate

Note1 Only reagent and concentration can

be entered in the Load and Unload Sections

a Reagent

Press [CHANGE REAGENT]. A list of the available reagents is displayed. Use the direction arrows, or use the number keys to type in the reagent number, to select the required reagent, then press [ENTER].

Note1 The user defined reagent name can be changed or added at this point.

Use the direction arrows [ ] and [ ] to move the cursor to select a user

defined reagent (reagents 43 - 54). Press the right hand function button,

[CHANGE NAME], and follow the instructions in section 4.2.2.

b Concentration (%)

Use the number keys to enter the concentration of the reagent to be used (1 - 100%). Use to differentiate between concentrations of alcohol for example. If not required, do not enter anything into this field.

c Set Uses

Use the number keys to enter the maximum number of times you wish to use the reagent before it must be changed (maximum = 99).





cba

The number will be shown on the Reagent Monitor Screen (accessed through the [MONITORING] button on the ‘Main Menu’ screen), and an optional warning to alert the operator can be selected in the Options Screen (accessed from the ‘Main Menu’ screen).

If not required, do not enter anything into this field.

d Time (minutes:seconds)

Use the number keys to enter the time required for the slides to be immersed in that reagent (minutes:seconds) (maximum 59 minutes and 59 seconds).

Press [ENTER] or use the direction arrow keys to move the cursor.

Note1 When Heater Stations are required, use 15 minutes for a trial batch and then

adjust the time as required to suit the laboratory conditions and process requirements.

e Limit

These settings help to optimise the speed and throughput of slides. The right hand function button switches through the following options:

1 NO MAXIMUM - use this setting to identify reagent steps where slides can be safely left for extended times. This setting allows urgent slides to be let through when multi-procedures are used.

2 CRITICAL - use this setting to give exact programmed times. For example, if the step is programmed for 30 seconds, the step will always be 30 seconds long.





d e

A78010402 Issue 438 A78010402 Issue 4 39

3 STANDARD - tolerance steps may be extended by up to 30% of the programmed time. For example, a 30 second step may actually take between 30 and 39 seconds (i.e. 30 secs + 30%).

Note1 If a Heater Station is selected, ‘Limit’ is automatically set to ‘NO MAXIMIUM’.

This cannot be changed.

f Agitate

Input the amount of agitation required at each step. The right hand function button switches through the following options:

1 STANDARD - agitation occurs when the slides are first placed in a reagent, and then every 2 minutes (approximately) apart from the last minute of a step

2 FREQUENT - agitation occurs when the slides are first placed in the reagent and then every minute throughout the step

3 CONTINUOUS - agitation occurs all the time. Throughput is significantly reduced if this option is selected

4 NONE - agitation is only used when the slides are first placed into the trough

Note1 If a Heater Station is selected, ‘AGITATE’ is automatically set to ‘NONE’. This

cannot be changed.

When the procedure information is complete and correct, press [EXIT] to return to the ‘Procedure Select’ screen.





f

Press [EXIT] again to return to the ‘How to Stain’ screen.

4.2.5 Using the Reagent Layout Facility

The Reagent Layout facility is used to assign the reagents to the most efficient layout to allow all selected procedures to be run.

Press [REAGENT LAYOUT] (a) from the ‘How To Stain’ screen to enter the ‘Reagent Layout 1’ screen.

Note1 The Reagent Layout 1 screen is not accessible if batches are currently being

processed by the stainer. A warning screen will be displayed if Reagent Layout 1 is selected when the instrument is running

4.2.6 Reagent Layout 1 Screen

This screen lists the procedures to be run simultaneously on the Varistain Gemini stainer.

Notes1 [ADD PROCEDURE] introduces

a new procedure and adds any reagents required into the free troughs on the stainer

2 [DELETE PROCEDURE] removes a procedure and allows the reagent troughs used by that procedure to be used for other reagents

3 [REAGENT LAYOUT 2] is used to view the current reagent layout and allows the operator to optimise the layout either manually or automatically

a


Define Reagents Define Protocols Reagent Layout Load Reagents


1. Use DEFINE REAGENTS to enter the names of reagents to be used2. Use DEFINE PROTOCOLS to enter the parameters for each

program step3. Use REAGENT Layout to select the procedures currently required4. Use LOAD REAGENTS to confirm each reagent has been placed in

position





6. When a batch has completed its staining protocol it will be placed behind and unload station door and its acknowledge button light will flash. Open the door and follow the instructions on the screen.

You cannot reprogram the instrument or alter its Reagent Layout while it is staining slides. Wait for all batches to complete and to be unloaded or use the Abort / Auto Return Facilities in the Monitoring screens.

Press EXIT to return to the previous Menu.

A78010402 Issue 440 A78010402 Issue 4 41

4 If a procedure is chosen and the required reagents will not fit into the existing Reagent Layout, a warning box will be displayed. If there are duplicate reagent troughs that can be removed so the procedure will fit, then the program will give the option to remove or retain the duplicates

Each procedure requires the following information to be entered:

a Batches per day (approximately)

b Load Door (A & B) information

c Unload Door (C & D) information

Use the direction arrows to move across to each field in turn.

a Batches per day

Use the number keys to enter the estimated number of times this procedure will be run in one day.

b Load doors

This option controls whether a procedure can start from each load door, and the priority that will be given to the procedure if the load acknowledge button ([A] or [B]) on the Load/Unload Panel is pressed.

The second function key (d) switches between the following options:

Varistain GeminiReagent Layout - 1 1/1/2000 10:42

Add Procedure Delete Procedure Reagent Layout 2

Loading Door options : Select from Standard, Urgent or Not Used.

Procedure Name and notes Batches /day Door A Door B Door C Door D

H&E Standard times 25 Standard StandardNo Print No PrintPAP Dr X preferred times 30 Urgent N/A N/A PrintPAS 2 Standard N/A No Print N/A

The reagents for each Load Door and each Unload Door are currently as shown:

Load Door A : Dry Storage Load Door B : Dry Storage Unload Door C : Xylene Unload Door D : Xylene





The procedure selected requires additional reagents which are not in the current Reagent Layout. There are insufficient duplicate pots to allow this procedure on to run on the stainer without removing one of the existing procedures. Press CANCEL and then use DELETE PROCEDURE to create the number of unallocated reagent pots required.


1 URGENT START - Use this to start an urgent procedure. It will ‘overtake’ any standard procedures in use if it can do so without compromising the programmed requirement of either procedure.

2 STANDARD START - Use this for routine procedures

3 DO NOT LOAD HERE - Use this selection (displayed as N/A) if you do not want to start a procedure from this door.

c Unload door information

This option controls the operation of the unload acknowledge keys [C] and [D].

The second function key (d) switches between the following options:

1 UNLOAD AND PRINT - Select this option if you require an automatic print-out of every batch as it is unloaded

2 UNLOAD - NO PRINT - Select this option if you do not require a print out

Note1 Print outs can be requested for

individual batches of slides from the ‘Acknowledge / Unload’ screen

3 DO NOT UNLOAD HERE - Use this selection (displayed as N/A) if you do not want a procedure to finish at this door.

Notes1 The cursor will not move to an invalid field - i.e. one where the reagent is

incorrect

2 The [ADD PROCEDURE] key will disappear if there are no more procedures that can use the reagents currently in the load and unload positions

d

Reagent Layout 2Unload and Print



H&E Standard times 25 Standard Standard No PrintPAP Dr X preferred times 30 Urgent N/A N/A PrintPAS 2 Standard N/A No Print N/A



No Print

c


Reagent Layout 2Urgent Start



H&E Standard times 25 Standard No Print No PrintPAP Dr X preferred times 30 Urgent N/A N/A PrintPAS 2 Standard N/A No Print N/A



Standard

d

ba


A78010402 Issue 442 A78010402 Issue 4 43

3 If a required procedure is not displayed on the ‘Reagent Layout 1’ selection list, it could be that the procedure uses load or unload reagents not used by the other listed procedures. To use the required procedure, either:

i Select each of the other procedures in turn and press [DELETE PROCEDURE] to remove them, or

ii Select each of the other procedures in turn and switch the selection for one load door (either [A] or [B]) and one unload door (either [C] or [D]) to N/A. (i.e. select ‘DO NOT LOAD HERE’ and ‘DO NOT UNLOAD HERE’)

When all the selections are complete, press [REAGENT LAYOUT 2] to view the current layout and optimise it if necessary.

4.2.7 Reagent Layout 2 Screen

The Shandon Varistain Gemini stainer allocates positions to all the reagents required for every procedure selected. This screen displays detailed information about the reagents.

The screen (see page 43) is divided into five areas showing the following information:

a Reagent layout - this shows the position of each reagent trough in the upper and lower levels of the stainer.

A & B Load positions / doors1 - 26 Reagent troughsH1 - H5 Heater positions / dry storageW1 - W6 Water wash positionsC & D Unload positions / doors

b Details about the upper level reagents

c Details about the lower level reagents

d A list of the procedures entered in the ‘Reagent Layout 1’ screen.

e Batch indicators showing the number and exact position of each batch that is being processed.

A 21 22 23 W1 W2 W3 H1 H2 H3 H4 H5 W4 W5 W6 24 25 26 B

1/1/2000 10:42

Print OptimiseAdd ProcedureComplete

UPPER LEVEL REAGENTS Set TimesTrough Reagent Conc.%Uses Used

A Dry Storage - 3

21 Bluing Reagent 7 4

22 Haematoxylin 7 *

23 Acid Alcohol 1 7 8 <

W1 Running Water - -



H1 Dry Storage - -

H2 Dry Storage - -

H3 Dry Storage - -

H4 Dry Storage - -

H5 Dry Storage - -




24 Periodic Acid 1 7 5

25 Schiffs Reagent 7 4

26

B Dry Storage - 4

batches PROCEDURESName / day Notes or staining steps

H&E 100 Dr xxxx procedure

PAS 6 Notes

UPPER LEVEL

REAGENTS

UPPER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 D

LOWER LEVEL REAGENTS Set TimesTrough Reagent Conc.%Uses Used

C Xylene - 3

1 Xylene

2 Xylene 7 *

3 Xylene 7 8 <

4 Alcohol 90 - -

5 Alcohol 70 - -

6 Alcohol - -

7

8 Haematoxylin - -

9

10 Alcoholic Eosin - -

11 Alcohol - -

12 Alcohol - -

13 Alcohol - -

14

15 Alcohol 100 - -

16 Alcohol - -

17 Xylene - -

18 Xylene 7 5

19 Xylene 7 4

20 Xylene 7 4

D Xylene - 4

H&E 20 Notes for procedure

a

b c d

18

^^

e

Note

1 If the cursor is in area (d) the notes for each procedure will be displayed (f).

f

A78010402 Issue 444 A78010402 Issue 4 45

A 21 22 23 W1 W2 W3 H1 H2 H3 H4 H5 W4 W5 W6 24 25 26 B

c

1/1/2000 10:42

Change Groups DuplicateMove


A Dry Storage - 3

21 Schiff’s Reagent - - -

22 Haematoxylin 7 *

23 Acid Alcohol 1 7 8 <




H1 Dry Storage - -

H2 Dry Storage - -

H3 Dry Storage - -

H4 Dry Storage - -

H5 Dry Storage - -




24 Acid Alcohol 1 7 5

25 Haematoxylin 7 4

26 Bluing Reagent 1 7 4

B Dry Storage - 4


H&E 20 Step 2

PAP 100 Step 5

PAS 6 Step 6


C Dry Storage - 3

1 Xylene - -

2 Xylene 7 *

3 Alcohol 7 8 <

4 Alcohol 90 - -

5 Alcohol 70 - -

6 Alcohol - -

7

8 Eosin - -

10 EA50 - -

11 Alcohol 95 - -

12 OG6 - -

13 Alcohol 95 - -

14

- -

16 Xylene - -

17 Xylene - -

18 Xylene 1 7 5

19 Xylene 7 4

20 Xylene 1 7 4

D Xylene - 4

9 Alcohol 100 - -

15 Alcohol 100

UPPER LEVEL

REAGENTS

UPPER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 D

18

^^

gae

b d

Reagent Layout 2 Function Buttons

The Function Buttons change depending on the status of the screen (see pages 43 and 44). This section describes all the Function Buttons that will be encountered in the Reagent Layout 2 screen.

[ADD PROCEDURE] will go back to the ‘Reagent Layout 1’ screen to allow another procedure to be added.

[CHANGE GROUPS]. Press this Function Button to access the following three Sharing Control screens - Reagent Sharing Control, Water Wash Sharing Control and Heater Sharing Control.

These functions can be used to make sure that certain positions are not be shared between different procedures, for example with gynaecological and non-gynaecological procedures.

Note1 A group is a set of troughs that are used interchangeably. Groups may be

created to improve performance or to extend reagent life

Place the cursor on a reagent position and press [CHANGE GROUPS] to display the ‘Reagent Sharing Control’ screen.

Place the cursor on a water wash position and press [CHANGE GROUPS] to display ‘Water Wash Sharing Control’ screen.

Place the cursor on a heater position (heater version only) and press [CHANGE GROUPS] to display the ‘Heater Sharing Control’ screen.

Note1 If the cursor is in area (b) or (c), details of the steps where the highlighted reagent is

used in each procedure will be displayed (g).

In the example shown, 100% alcohol in reagent troughs 9 and 15 are used in step 2 of the H&E procedure, step 5 of the PAP and step 6 of the PAS procedure.

A78010402 Issue 446 A78010402 Issue 4 47

a ‘Reagent Sharing Control’ screen

This screen shows information about the sharing of reagents:

i The reagent that was selected when [CHANGE GROUPS] was pressed (b)

ii All the procedures that use that reagent (a)

iii The steps where the reagent is used (c)

iv The troughs that contain the reagent (d)

v Any spare troughs (e).

Note

1 Only one spare trough will be displayed at any one time. If there are no spare troughs available, the spare trough box (e) will not be displayed.

The example screen shown shows that Xylene (b) is used in steps 2, 3 and 4 of the H&E procedure and steps 3, 5 and 6 of the PAS procedure (a & c). Troughs 3, 7 and 12 contain Xylene (d) and there is at least one spare trough (e).

To make sure that a particular trough of reagent is not shared between procedures, use [ ] and [ ] to highlight the trough to be changed and move the ‘xxx’ to the spare trough using [ ] and [ ].

The instrument will automatically allocate a spare trough for this reagent.

In the example, step 5 of the PAS procedure has been reallocated to trough 10.

Reagent Sharing Control 1/1/2000 10:42

GROUPS OF REAGENT POTSPre-Stain Xylene -%

No Procedure Name & Notes Step

1 H&E 2 xxx 3 xxx 4 xxx

2 PAS 3 xxx 5 6 xxx

3 127

xxx

Reagent Sharing Control 1/1/2000 10:42




2 PAS 3 xxx 5 6 xxx

3 10127

xxx

b ‘Water Wash Sharing Control’ screen

This screen shows all the water wash stations and which steps of the procedures use them.

If ‘xxx’ is displayed, that Water Wash Station is to be used. If nothing is displayed the Water Wash Station will not be used.

In the example, Water Wash Station W2 will not be used for H&E step 10, and W1 will not be used for PAS step 12.

To change the setting for the Water Wash Stations, use the direction arrows to the particular step and Water Wash Station, and press [USE] or [DO NOT USE].

c ‘Heater Sharing Control’ screen

This screen works in the same way as the ‘Water Wash Sharing Control’ screen for the allocation of heater stations on the heater version of the Shandon Varistain Gemini.

The ‘Heater Sharing Control’ screen also controls the use of ‘dry storage’ steps (if selected) on both versions on the Shandon Varistain Gemini.

[CHANGE OPTIONS] is displayed when the door positions are highlighted. If pressed, the ‘Reagent Layout 1’ screen will be shown.

[COMPLETE]. Press this Function Button when you are satisfied with the reagent layout, before you move on to the ‘Load Reagents’ screen.

[DELETE] is only available if any duplicates are highlighted in the ‘Reagent Layout 2’ screen. It removes a selected duplicate reagent from the layout. It will only remove reagents that are duplicated, and will not delete the last trough of any reagent group.

In the example shown on page 44, either reagent 9 or reagent 15 can be deleted, but not both.

Use Do Not Use

Water Wash Sharing Control 1/1/2000 10:42

WATER WASH STATIONS

RUNNING WATER STEPSNo Procedure Name & Notes Step

1 H&E 8 xxx xxx xxx xxx xxx 10 xxx xxx xxx xxx xxx 12 xxx xxx xxx xxx xxx xxx 14 xxx xxx xxx xxx xxx xxx 16 xxx xxx xxx xxx xxx

2 PAS 8 xxx xxx xxx xxx xxx xxx 10 xxx xxx xxx xxx xxx xxx 12 xxx xxx xxx xxx xxx 15 xxx xxx xxx xxx xxx 17 xxx xxx xxx xxx xxx xxx

W1 W2 W3 W4 W5 W6

xxxReagent Sharing Control 1/1/2000 10:42




2 PAS 3 xxx 5 xxx 6 xxx

3 127

a edcb

xxx

A78010402 Issue 448 A78010402 Issue 4 49

[DUPLICATE] allows a reagent to be copied to an empty position. The original reagent and any duplicates will be grouped, and will be shared for any steps where that reagent is required.

In the example shown, the 100% alcohol in position 9 has been copied to position 15. The steps where this reagent is used will now use either trough 9 or trough 15.

[MOVE] allows a reagent to be moved to an empty position if required.

[OPTIMISE]. Press this Function Button to access the following three Function Buttons:

a [AUTO ALLOCATE] first removes all existing duplicates. Then it adds the best duplicates in positions to optimise the performance of the instrument for a day’s work.

Note

1 If a day’s work can be carried out without a replenishment stop or any bottlenecks, [AUTO ALLOCATE] will not make any changes and may leave some troughs empty.

b [AUTO ARRANGE] optimises the positions of the current reagents for the best possible performance. It will not add or remove duplicates.

c [REMOVE DUPLICATES] optimises the reagent layout for the cheapest option. All duplicates are removed - only one trough of each required reagent is left.

[PRINT] will print the reagent layout if a printer is connected.

4.2.8 To Load Reagents

USE GOOD LABORATORY PRACTICE WHEN HANDLING REAGENTS. BE AWARE OF ANY HAZARDS ASSOCIATED WITH THEM. ALWAYS USE COMMON SENSE.

Refer to ‘Safety and Chemicals’ section on page 7 before the instrument is filled with reagents.

DO NOT FILL HEATER STATION TROUGHS WITH REAGENTS.

DO NOT SPLASH OR POUR REAGENTS NEAR TO THE HEATER STATION TROUGHS.

DO NOT PLACE STANDARD REAGENT TROUGHS INTO THE HEATER POSITIONS - REAGENTS MAY BE INADVERTENTLY HEATED AND THE INSTRUMENT MAY NOT OPERATE CORRECTLY.

Before any procedures can be run, the reagents allocated by the Reagent Allocation programme must be loaded into the instrument in the correct positions.

Reagent Monitoring Screen

From the ‘How To Stain’ screen, press [LOAD REAGENTS] to enter the ‘Reagent Monitoring’ screen.

The ‘Reagent Monitoring’ screen (see page 50) shows the following information:

i The exact positions of each batch of slides (a)

ii ‘<’ shows if any reagent has exceeded its set number of uses (b)

iii The positions where each reagent must be loaded to ensure correct operation of the instrument (c)

iv ‘>’ shows that a reagent has not been loaded in position (i.e. [CONFIRM LOADED] has not been pressed) (d)

v How many weeks the Charcoal Filter has been installed (e)

WARNING


1. Use DEFINE REAGENTS to enter the names of reagents to be used2. Use DEFINE PROTOCOLS to enter the parameters for each program step3. Use REAGENT LAYOUT to select the procedures currently required4. Use LOAD REAGENTS to confirm each reagent has been placed in position



How to Stain 1/1/2000 10:42

Define Reagents Load ReagentsReagent LayoutDefine Protocols

Varistain Gemini

A78010402 Issue 450 A78010402 Issue 4 51

A 21 22 23 W1 W2 W3 H1 H2 H3 H4 H5 W4 W5 W6 24 25 26 B

18

^^

1/1/2000 10:42

Batch MonitoringStopConfirm Loaded


A> Dry Storage

21> Bluing Reagent 7 4

22> Haematoxylin 7 4

23> Acid Alcohol 1 7 8




H1 Dry Storage - -

H2 Dry Storage - -

H3 Dry Storage - -

H4 Dry Storage - -

H5 Dry Storage - -




24> Periodic Acid 1 7 5

25> Schiffs Reagent 7 4

26>

B> Dry Storage


H&E 20 Notes for procedure

H&E 100 Dr xxxx procedure

PAS 6 Notes

UPPER LEVEL

REAGENTS

UPPER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 D


C> Xylene

1> Xylene

2> Xylene 7 *

3> Xylene 7 8 <

4> Alcohol 90 - -

5> Alcohol 70 - -

6> Alcohol - -

7

8> Haematoxylin - -

9

10> Alcoholic Eosin - -

11> Alcohol - -

12> Alcohol - -

13> Alcohol - -

14

15> Alcohol 100 - -

16> Alcohol - -

17> Xylene - -

18> Xylene 7 5

19> Xylene 7 4

20> Xylene 7 4

D> Xylene

ce

ba

Reagent Monitoring

HARDWARE QUALITY COUNTS

Limit CountCharcoal Filter Replacement 13 14 <

d

To Load Reagents into the Shandon Varistain Gemini

Carefully pour the required reagent into a reagent trough to the marked fill line.

Note1 The reagent troughs will hold

320ml of reagent - enough to cover 1 - 20 slides.

Place the trough in the correct position in the instrument.

Notes1 The position number of each

reagent trough is marked in the instrument.

2 Rotate the Turntable when the lower level reagents are loaded, taking care to avoid spillage.

As each reagent is placed in position, press [CONFIRM LOADED] to acknowledge that the reagent is in the correct position. (The ‘>’ displayed next to each reagent position will disappear as soon as the reagent has been acknowledged. (See (c) on ‘Reagent Monitoring screen on page 50))

REAGENTS MUST BE PLACED IN THE CORRECT POSITIONS AND [CONFIRM LOADED] PRESSED FOR THE INSTRUMENT TO WORK CORRECTLY.

Note

1 If the loading of reagents has not been confirmed before the procedure is started, the red Quality Control Warning screen (see Section 4.2.11) will automatically be displayed. A ‘>’ next to a reagent means that the loading of that reagent has not yet been confirmed.

When the cursor is in either of the reagent boxes (a), press [CONFIRM LOADED] to confirm the reagent.

QUALITY CONTROL WARNING

<<<<<<< QUALITY CONTROL WARNING >>>>>>>

The Procedure selected cannot be run with-out exceeding the limits set within the Define Procedures screen, for reagents marked <.

To replenish reagents use the arrow keys to select the reagents and press CLEAR after each replenishment.

Press CONTINUE when all warnings have been cleared. Press OVERRIDE to ignore any remaining warnings. The stainer will restart automatically, including the latest batch.

Pressing RESTART will only continue staining the interrupted batches. Press CANCEL to abort the loading of the latest batch.

A 21 22 23 W1 W2 W3 H1 H2 H3 H4 H5 W4 W5 W6 24 25 26 B

Cancel OverrideConfirm Loaded


A> Dry Storage







H1 Dry Storage - -

H2 Dry Storage - -

H3 Dry Storage - -

H4 Dry Storage - -

H5 Dry Storage - -






26>

B> Dry Storage



The Procedure selected cannot be run without exceeding the limits set within the Define Procedures screen, for reagents marked <.


Press CONTINUE when all warnings have been cleared. Press OVERRIDE to ignore any remaining warnings. The stainer will restart automatically, includ-ing the latest batch.

Pressing RESTART will only continue staining the inter-rupted batches. Press CANCEL to abort the loading of the latest batch.

UPPER LEVEL

REAGENTS

UPPER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 D


C> Xylene

1> Xylene

2> Xylene 7 *

3> Xylene 7 8 <

4> Alcohol 90 - -

5> Alcohol 70 - -

6> Alcohol - -

7

8> Haematoxylin - -

9


11> Alcohol - -

12> Alcohol - -

13> Alcohol - -

14

15> Alcohol 100 - -

16> Alcohol - -

17> Xylene - -

18> Xylene 7 5

19> Xylene 7 4

20> Xylene 7 4

D> Xylene

Reagent Monitoring



a

A78010402 Issue 452 A78010402 Issue 4 53

A 21 22 23 W1 W2 W3 H1 H2 H3 H4 H5 W4 W5 W6 24 25 26 B

18

^^

1/1/2000 10:42

Reagent MonitoringStopAuto Return

Batch Start Procedure Step Reagent Time Pot End Time & Place Priority # Time

18 10:55 1 H&E 7 Running Water 1:23 W3 11:21 D Standard19 11:10 2 PAS 2 Xylene 0:50 2 11:40 C Standard

UPPER LEVEL

REAGENTS

UPPER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 D

b

Batch Monitoring

a g kjihfedc

19

^^

iii The time each batch started (c)

iv The procedure and procedure number being used to stain each batch of slides (d)

v The procedure step that the batch has reached (e)

vi The reagent that the batch has reached (f)

vii The time remaining at that step (g)

viii The trough position where the batch of slides has reached (h)

ix The time when the batch should finish its procedure (i)

x The output door where the batch will finish (j)

xi The priority of the batch (k)

Note1 When loading another batch of

slides, if the instrument calculates that during a multiple batch run, a reagent will exceed its set uses, the red Quality Control Warning Screen (see Section 4.2.11) will be displayed. The reagent to be replenished will be marked with a ‘<’ next to it. Follow the on-screen instructions to replenish the reagent. Once the reagent has been replenished, press [CLEAR].

2 The count will not necessarily be reset to zero. The number displayed on the red Quality Control Warning screen is the total number of times that a reagent trough will have been used by the time all batches have completed their staining procedure (including the batch being loaded). If [CLEAR] is pressed, all the uses that have already happened will be removed, but those that are still to occur are left.



The procedure selected cannot be run with-out exceeding the limits set within the Define Procedures screen, for reagents marked <.

To replenish reagents press STOP and follow the on-screen prompts. When the stainer has stopped use the arrow keys to select reagents and press CLEAR after each replenishment.

Press CONTINUE when all warnings have been cleared.

Press OVERRIDE to remove the warning screen and continue to stain using the current reagents.

A 21 22 23 W1 W2 W3 H1 H2 H3 H4 H5 W4 W5 W6 24 25 26 B

18

^^

1/1/2000 10:42

Cancel OverrideRestartClear


A> Dry Storage







H1 Dry Storage - -

H2 Dry Storage - -

H3 Dry Storage - -

H4 Dry Storage - -

H5 Dry Storage - -






26>

B> Dry Storage



The procedure selected cannot be run without exceeding the limits set within the Define Procedures screen, for reagents marked <.

To replenish reagents press STOP and follow the on-screen prompts. When the stainer has stopped use the arrow keys to select reagents and press CLEAR after each replenishment.

Press CONTINUE when all warnings have been cleared.

Press OVERRIDE to remove the warning screen and continue to stain using the current reagents.

UPPER LEVEL

REAGENTS

UPPER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 D


C> Xylene

1> Xylene

2> Xylene 7 *

3> Xylene 7 8 <

4> Alcohol 90 - -

5> Alcohol 70 - -

6> Alcohol - -

7

8> Haematoxylin - -

9


11> Alcohol - -

12> Alcohol - -

13> Alcohol - -

14

15> Alcohol 100 - -

16> Alcohol - -

17> Xylene - -

18> Xylene 7 5

19> Xylene 7 4

20> Xylene 7 4

D> Xylene

Reagent Monitoring



Batch Monitoring Screen

From the ‘Reagent Monitoring’ screen, press [BATCH MONITORING] to enter the ‘Batch Monitoring’ screen.

The ‘Batch Monitoring’ screen will be used regularly to view the progress of each batch of slides. The following information is displayed:

i The batch numbers of the slides that are currently being stained (a)

ii The exact positions of each batch of slides (b)

A78010402 Issue 454 A78010402 Issue 4 55

[AUTO RETURN] will return a specified batch to the trough behind the load door. Use the direction arrows to move the cursor to highlight the required batch and press [AUTO RETURN].

Note1 The [AUTO RETURN] button on the Load / Unload Touch Panel (paragraph

3.3.3) will return only the last batch of slides loaded onto the instrument.

[STOP] (on both ‘Batch Monitoring’ and ‘Reagent Monitoring’ screens) is used to stop a batch of slides. The instrument will finish its current movement and will then stop the time countdowns in all procedures to allow the batch to be retrieved.

To use [STOP]:

1 Wait until the instrument places all the batches of slides in safe stop positions.

2 Press [STOP].

3 Press [STOP NOW] to confirm that you do want to stop the instrument, or [DO NOT STOP] to return to the ‘Batch Monitoring’ screen.

4 Use the direction arrows to select the batch required and press [ABORT] when the slides are in a convenient position to be removed from the instrument.

ANY BATCHES THAT ARE ABORTED DURING A PROCEDURE MUST BE REMOVED FROM THE INSTRUMENT.

5 Remove the correct batch from the instrument and close the doors.

6 Press [RESTART] to resume the staining procedures of all other batches in the instrument.

CHECK THAT EVAPORATION COVERS ARE REMOVED FROM ALL TROUGHS BEFORE USE.

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

a

4.2.9 To Stain Slides

Once all the reagents have been correctly placed in the stainer, slides can be stained.

The Load Acknowledge LED (on the Load Buttons [A] and [B]) will be lit if that load door can be used (a).

Open the sliding door at either A or B and place a basket and carrier into the loading trough directly behind the door (marked A and B on the base adjacent to the troughs). The ‘Loading’ screen will automatically appear on the monitor. Use the direction arrows [ ] and [ ] to select the required procedure.

THE SLIDING DOORS ARE ONLY INTENDED FOR THE OPERATOR TO ACCESS THE LOAD AND UNLOAD TROUGHS DIRECTLY BEHIND THE DOORS. DO NOT REACH FURTHER INTO THE INSTRUMENT - THE ARM CAN MOVE SUDDENLY.

THE ARM AND BASKET CAN MOVE SUDDENLY BOTH HORIZONTALLY AND VERTICALLY.

DO NOT REST HANDS ON TOP OF THE TROUGHS.

DO NOT TOUCH THE ARM MECHANISM IN THE CENTRE OF THE INSTRUMENT.

Close the doors and if the procedures are set up, press [A] or [B] on the Load / Unload Panel to acknowledge.

Alternatively, select [STANDARD START] or [URGENT START] from the ‘Loading’ screen.

Note1 A commonly used procedure can be set as the default program. This allows the

operator to open one of the load doors, A or B, load the slides, close the door and press either [A] or [B] on the Load / Unload Panel to acknowledge.

Door A - Loading Slides - Batch 57

No.Procedure name / Notes

1 PAP Dr xxxx special PAP

Close the load door, select the required procedure and press the appropriate START.

1/1/2000 10:42

Standard Start CancelSet As DefaultUrgent Start

PROCEDURENote for selected procedure

4 PROCEDURE Note for selected procedure

Varistain Gemini

A78010402 Issue 456 A78010402 Issue 4 57

The default procedure will start automatically on Standard or Urgent, depending on the Load Door Option selected in the ‘Reagent Layout 1’ screen.

To set a procedure as the default procedure, move the cursor using the direction

arrows [ ] and [ ]. Press [SET AS DEFAULT] in the ‘Loading’ screen.

The default procedure will be highlighted when the ‘Loading’ screen is first displayed. If no procedure is set as default, the first procedure on the list will be highlighted.

4.2.10 To Unload Slides

Once a batch of slides has been stained and the batch of slides is ready to be removed from the instrument, the Unload Acknowledge LED on the relevant Unload Button ([C] or [D]) will flash (a) to show which door should be opened to retrieve the slides.

In the example shown, the LED on Unload Button [C] is flashing (a). The batch of slides in this case is in the trough behind Unload Door [C].

Open the Unload Door and remove the batch of slides. Press the relevant Unload Button to acknowledge that the slides have been removed and that the Unload Door is now free for another batch.

Alternatively, [ACKNOWLEDGE] can be pressed in the ‘Unload’ screen.

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

AC

BD

AUTORETURN

a

4.2.11 Quality Control Warning Screen

The Quality Control Warning Screen is automatically displayed whenever there is a conflict that will prevent the instrument loading a batch of slides and staining it according to its set uses for each reagent.

Follow the instructions given in the Quality Control Warning box to clear the conflict.

An example is shown here.

Note

1 If a ‘<’ is displayed in other screens, then a Quality Control conflict will exist by the time the batch to be loaded has run through its procedure.

A 21 22 23 W1 W2 W3 H1 H2 H3 H4 H5 W4 W5 W6 24 25 26 B

18

^^

1/1/2000 10:42

Cancel OverrideConfirm Loaded


A> Dry Storage







H1 Dry Storage - -

H2 Dry Storage - -

H3 Dry Storage - -

H4 Dry Storage - -

H5 Dry Storage - -






26>

B> Dry Storage



The Procedure selected cannot be run without exceeding the limits set within the Define Procedures screen, for reagents marked <.

To replenish reagents use the arrow keys to select the rea-gents and press CLEAR after each replenishment.

Press CONTINUE when all warnings have been cleared. Press OVERRIDE to ignore any remaining warn-ings. The stainer will restart automatically, including the latest batch.

Pressing RESTART will only continue staining the inter-rupted batches. Press CANCEL to abort the loading of the latest batch.

UPPER LEVEL

REAGENTS

UPPER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

LOWER LEVEL

REAGENTS

C 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 D


C> Xylene

1> Xylene

2> Xylene 7 *

3> Xylene 7 8 <

4> Alcohol 90 - -

5> Alcohol 70 - -

6> Alcohol - -

7

8> Haematoxylin - -

9


11> Alcohol - -

12> Alcohol - -

13> Alcohol - -

14

15> Alcohol 100 - -

16> Alcohol - -

17> Xylene - -

18> Xylene 7 5

19> Xylene 7 4

20> Xylene 7 4

D> Xylene

Reagent Monitoring





The Procedure selected cannot be run with-out exceeding the limits set within the Define Procedures screen, for reagents marked <.


Press CONTINUE when all warnings have been cleared. Press OVERRIDE to ignore any remain-ing warnings. The stainer will restart automati-cally, including the latest batch.

Pressing RESTART will only continue staining the interrupted batches. Press CANCEL to abort the loading of the latest batch.

A78010402 Issue 458 A78010402 Issue 4 59

4.3 HOUSEKEEPING TASKS

Select [OPTIONS] from the Main Menu to access the Housekeeping tasks.

4.3.1 To Change the Time and Date

Press [SET CLOCK] to display the ‘Set Clock’ screen.

Press [12 / 24 Hour Clock] to switch between 12 and 24 hour clock format.

Press [DAY <-> MONTH] to switch between the date options of Day / Month or Month / Day.

Use the direction arrows to move to the time and date settings to change them. Use the number keypad on the Power Supply Unit to enter the new time and date.

Note1 The ‘Set Clock’ screen cannot be accessed while a staining procedure is in

progress.

4.3.2 Quality Control Settings

Select [WARNINGS] and then select [QUALITY CONTROL].

Press [ON / OFF] to turn the Reagent Usage warnings on or off.

If ‘On’ is chosen, warnings are shown on the ‘Reagent Monitoring’ screen if any of the reagents have reached the usage limits set when the procedure was defined.


Press SET CLOCK to alter the time or date.Press WARNINGS for Quality Control and audible warnings.Press DATA MANAGEMENT to use the disc drive or to obtain printouts.Press ACCESS CODES to set or alter the protection code.

Options 1/1/2000 10:42

Set Clock Access CodeData ManagementWarnings

Varistain Gemini

Select display format

Time 24 Hour Date Day / Month

Select time and date

Hours 10 Minutes 42 Day 1 Month 1 Year 2000

Press SET to change the clock to the new values

Set Clock 1/1/2000 10:42

Set Day <-> Month12/24 Hour Clock

Varistain Gemini

The Charcoal filter life warning level can be set in this screen (in weeks). It is recommended that the filter is replaced at least every 13 weeks.

If the filter has exceeded the limit set, a warning will be displayed at the start of each staining run until the filter is replaced.

Press [ON / OFF] to turn the warning off if a fume extraction adaptor is fitted.

4.3.3 To Change the Audio Setup

Select [WARNINGS] and then select [AUDIO].

Use the direction arrows to select the required event.

Press [DEMONSTRATE] to hear the warning sound chosen.

Press [NEW TUNE] to select a new warning sound. A selection of warning sounds will be displayed.

Press [SILENCE] to turn off the warning sound for the chosen event.

Press [CONTINUOUS] to set the number of repeats to continuous. The warning sound will continue until [ ] on the PSU is pressed.

Note

1 To remove ‘Continuous’ from the repeat column, enter another number.

Reagent Usage Warning On

Filter Replacement Warning Interval 13 weeks

Quality Control 1/1/2000 10:42

On / Off

Varistain Gemini

Please select the required option and then press DEMONSTRATE to check the audible warning given when each event occurs. Press NEW TUNE to alter the selection. Press Silence to turn off the audible warning for that event.

Event Sound Repeats1 Start Up Complete Signature Tune 02 A keypanel key is pressed Short blip 03 A load door is left open for 15 seconds 4 falling notes 24 A load door is left open for 30 seconds 4 falling notes 105 A batch finishes staining 4 rising notes 06 A Load / Unload door is opened in error 4 falling notes 07 A Basket Transport Arm error occurs Error notes Continuous8 A Main Door is opened in error Error notes Continuous9 Load sequence incomplete for 30 seconds4 falling notes 2

Audio Configuration 1/1/2000 10:42

Demonstrate Continuous SilenceNew Tune

Varistain Gemini

A78010402 Issue 460 A78010402 Issue 4 61

4.3.4 To Print Information

Information can be printed out on an IBM compatible Centronics parallel printer.

Select [OPTIONS] from the Main Menu, and then select [DATA MANAGEMENT].

Select [PRINTOUTS] from the Options, Data Management screen.

Use the direction arrows [ ] and [ ] or the number keys to select the required option, and then press the correct function key to choose the required destination for the information:

Note

1 [PRINTER] will print the information.

2 [ON-SCREEN] will display the information on the monitor

3 [DISC DRIVE] will save the information to disc in the form of a text file that can be read and edited using a standard PC text editor. (Use standard DOS compatible 1.44Mb 31⁄2 inch floppy disks.)

4.3.5 To Save or Retrieve Information From a Floppy Disk

The Shandon Varistain Gemini stainer has a 31⁄2 inch disk drive to allow the operator to save particular procedures or records of specific batches of slides, and also to download standard procedures and information to set up the instrument.

Use standard DOS compatible 1.44Mb 31⁄2 inch floppy disks.


Press DISC DRIVE to store or retrieve data using a floppy disc.

Press PRINTOUTS to obtain printouts of Event Logs, Procedures and Reagent Layouts. All printouts can be previewed on screen before printing

Data Management 1/1/2000 10:42

Disc Drive Printouts

Varistain Gemini

Please select the required option and then press PRINTER, ON-SCREEN or DISC DRIVE.

1 Print a copy of a Procedure2 Print all Procedures3 Print Reagent Layout and Door Configuration4 Print Event Log for a specific batch of slides5 Print Event Logs for all batches of slides6 Print the entire Instrument Setup

Printout Options 1/1/2000 10:42

Disc DriveOn-ScreenPrinter

Varistain Gemini

Select [OPTIONS] from the Main Menu, and then select [DATA MANAGEMENT].

Select [DISC DRIVE] from the Options, Data Management screen.

Use the direction arrows [ ] and [ ]

or the number keys to select the required

option, and then press [START] to access

the disc drive.

Note

1 The file saved can only be used by a Shandon Varistain Gemini. If a record of the instrument settings is required, choose the Printouts option.

4.3.6 Access Codes

Access codes can be used as a security measure when programming the instrument, changing the Reagent Layout or items 6 - 9 from the Disc Drive Options menu.

Select [OPTIONS] from the Main Menu, and then select [ACCESS CODES].

To set an access code, press [SET ACCESS CODE].

Use the number keypad to enter a 4 digit code. Press [ENTER]. Re-enter the code followed by [ENTER] when prompted. The access code is now active.

To change an access code, press [SET ACCESS CODE] and enter the old access code followed by [ENTER].

Please select the required option and then press START

1 Write a copy of a Procedure to a floppy disc2 Write all Procedures to a floppy disc3 Write Reagent Layout and Door Configuration to a floppy disc4 Write the entire Instrument Setup to a floppy disc5 Read a Procedure from a floppy disc6 Read all Procedures from a floppy disc7 Read Reagent Layout and Door Configuration from a floppy disc8 Read the entire Instrument Setup from a floppy disc9 Load new features from a floppy disc

Disc Drive Options 1/1/2000 10:42

Start

Varistain Gemini

The Access Code is not set

Access Codes 1/1/2000 10:42

Set Access Code

Varistain Gemini

A78010402 Issue 462 A78010402 Issue 4 63

Enter a new 4 digit code followed by [ENTER] when prompted. This new code must be entered again before the access code is changed.

To clear an access code, enter the old code and press [ENTER] twice when prompted for a new code.

If you forget the password, press [CANCEL ACCESS CODE] and wait for 2 days.

Notes

1 The time will count down on the top of each screen.

2 To keep the access code and stop the countdown, press [ * ] from any screen.

To use the access code, enter the code whenever the screen requests it. Once the access code has been used, press [ * ] to re-enable it.

The Access Code is set

Access Codes 1/1/2000 10:42

Set Access Code Cancel Access Code

Varistain Gemini CLEANING AND MAINTENANCE

For the continued safe and efficient operation of the instrument, it is important that regular inspection of the instrument during the following cleaning and maintenance procedures is carried out.

IF HAZARDOUS MATERIAL IS SPILT ON, OR INSIDE, THE INSTRUMENT, THE USER SHOULD CARRY OUT THE APPROPRIATE DECONTAMINATION.

CLEANING OR DECONTAMINATION METHODS, OTHER THAN THOSE RECOMMENDED IN THE OPERATOR GUIDE, SHOULD BE CHECKED WITH A THERMO AGENT TO ENSURE THAT THE PROPOSED METHOD WILL NOT DAMAGE THE EQUIPMENT.

5.1 CLEANING

TURN OFF THE POWER AND REMOVE THE MAINS PLUG FROM THE MAINS SUPPLY BEFORE CLEANING THE SHANDON VARISTAIN GEMINI STAINER.

ALWAYS WIPE UP ANY SPILLS IMMEDIATELY. IN THE EVENT OF A MAJOR SPILLAGE, DISCONNECT THE INSTRUMENT FROM THE MAINS SUPPLY WITHOUT DELAY AND DO NOT RECONNECT AND SWITCH ON UNTIL THE INSTRUMENT HAS BEEN THOROUGHLY DRIED OUT AND CHECKED BY A SERVICE ENGINEER.

5.1.1 Cleaning the Stainer

Clean the instrument regularly as part of your normal housekeeping routine.

Any accidental spillage of stains on the touch control panel should be removed by wiping with alcohol immediately.

To clean the inside and outside of the Shandon Varistain Gemini stainer, wipe with a cloth damped in soapy water, then polish with a dry cloth. DO NOT USE EXCESSIVE LIQUID.

WARNING

A78010402 Issue 464 A78010402 Issue 4 65

5.1.2 Cleaning the Power Supply Unit

The case of the Power Supply Unit only requires wiping with a clean, dry cloth.

Any accidental spillage of stains on the touch control panel should be removed by immediately wiping with a cloth and a small amount of alcohol.

POTENTIALLY LETHAL VOLTAGES ABOVE 110V A.C. ARE PRESENT INSIDE THE POWER SUPPLY UNIT. DO NOT REMOVE ANY ACCESS COVERS.

5.1.3 Cleaning the Reagent Troughs

The plastic reagent and water wash troughs supplied with this instrument have been found suitable for use with most solutions and reagents used in staining routines.

Solutions containing iodine may be used in the troughs, although ageing is accelerated and the troughs will need to be replaced at approximately six-monthly intervals.

5.1.4 Cleaning the Heater Station Troughs (where applicable)

Use an appropriate wax removing solvent to clean away all traces of wax from the Heater Station troughs.

5.1.5 Washing Procedures

It is recommended that the troughs, baskets and carriers are washed at a maximum temperature of 45°C and that they are allowed to drain or hand dry.

Do not use a dishwasher.

Do not use detergents that contain bleach.

5.1.5 Monitor

THE MONITOR IS NOT RESISTANT TO SOLVENTS.

5.2 MAINTENANCE

The Shandon Varistain Gemini stainer requires annual serving by a qualified service engineer.

WARNING

WARNING

WARNING

TROUBLE SHOOTING6.1 INTRODUCTION

Correct service and maintenance is essential for the long term serviceability of precision engineered products such as the Shandon Varistain Gemini stainer. Only a qualified Thermo service engineer must set up and service the instrument, to ensure future reliability and consistency of performance.

Table 1 shows remedial action to be taken if the Shandon Varistain Gemini stainer does not operate correctly.

Poor staining may be due to:

• Varying fill levels - the slides are not fully covered by reagents during the staining process

• Wax is not completely removed from the specimen, • Inadequate fixation of the specimen• Inadequate dehydration of the specimen• Inadequate clearing of the specimen• Inadequate impregnation of the specimen with paraffin wax

The following tables list some typical problems and their remedies that may occur during Haematoxylin and Eosin (H&E) or Papanicolaou (PAP) staining:

Table 2 - NUCLEAR STAINING PROBLEMS

Table 3 - CYTOPLASMIC STAINING PROBLEMS

Table 4 - MISCELLANEOUS STAINING PROBLEMS.

Table 5 - TROUBLESHOOTING HAEMATOXYLIN STAINING

Table 6 - TROUBLESHOOTING PAPANICOLAOU STAINING

IF A WARNING SCREEN IS DISPLAYED AT ANY TIME, FOLLOW THE INSTRUCTIONS ON THE SCREEN.2 If the cursor is in area (b) or (c), details of the steps where the highlighted reagent is used in each procedure will be displayed (g).

In the example shown on page 43, 100% alcohol in reagent troughs 9 and 15 are used in step 2 of the H&E procedure, step 5 of the PAP and step 6 of the PAS procedure.

A78010402 Issue 466 A78010402 Issue 4 67

TABLE 1 - EQUIPMENT STATUS CHECKS

SYMPTOM CAUSE REMEDY

1 Standby LED not lit on the PSU

a Power Supply Unit not connected

b Power Supply Unit not switched on

c Mains fuse blown

d Instrument fuse blown

a Check the connections

b Switch on PSU

c Replace mains fused Replace the instrument

fuse

Note: Only an authorised

technician should replace fuses

2 Arm does not move a Main door is open

b Load / Unload door is open

a Make sure that both main doors are closed

b Make sure that the Load and Unload doors are closed

TABLE 2 - NUCLEAR STAINING PROBLEMS(+ = H&E only; * = PAP only)


1 No nuclear staining or staining too light

a Haematoxylin exhausted

b Differentiation too strong (over-differentiation)

c Chlorine in tap water

d Staining time too short in haematoxylin

*e Cells air dried

f Haematoxylin too weak

g Haematoxylin expired

+h Autopsy specimen

i Autolysis started

+j Prolonged specimen storage in formalin

k Haematoxylin prepared inaccurately

l Stain cold

m pH of haematoxylin incorrect

n Incomplete hydration

o Slides allowed to dry after initial hydration

a Replace with fresh haematoxylin

b Dilute differentiating solution Remove, or decrease time

c Use bluing reagent Use distilled water

d Increase staining time

e Irretrievable. Fix promptly

f Choose the type of haematoxylin with the correct strength

g Replace with fresh haematoxylin

h Increase time in haematoxylin

i Irretrievable. Fix promptly

j Increase time in haematoxylin

k Check dye content and adjust formula accordingly

Check formula to assure that correct dilution is done

l Use warmer stains. The colder they are, the slower they work

m Adjust pH with glacial acetic acid

n Thoroughly hydrate slides prior to immersion in haematoxylin

o Do not let slides dry from initial submersion through coverslipping

A78010402 Issue 468 A78010402 Issue 4 69

TABLE 2 - (Continuation)(+ = H&E only; * = PAP only)


+p Deparaffinisation incomplete

+q Over decalcification

*r Incomplete removal of Carbowax

p Return to xylene to complete deparaffinisation

q Immerse in saturated mercuric chloride for 10 min.

r Soak slides in 95% alcohol for 10 min. prior to staining

2 Nuclear detail lacking (smudged, grey)

No “crisp” chromatin, bland appearance

a Haematoxylin exhausted

b Chlorine in tap water

*c Cells air dried

d pH of haematoxylin too high

e Incomplete hydration

f Slides allowed to air dry after initial hydration

+g Deparaffinisation incomplete

+h Overdecalcification

*i Incomplete removal of carbowax

j Autolysis started

k Bluing step ineffective

+l Prolonged specimen storage in formalin

a Replace with fresh haematoxylin

b Use distilled water

c Irretrievable. Fix promptly

d Adjust pH with glacial acetic acid

e Thoroughly hydrate slides prior to immersion in haematoxylin

f Do not let slides dry from initial submersion through coverslipping

g Return to xylene to complete deparaffinisation

h Immerse in saturated mercuric chloride for 10 minutes

i Soak slides in 95% alcohol for 10 min. prior to staining

j Irretrievable. Fix promptly

k Check pH pf bluing reagent and change if necessary

Increase time in bluing reagent

l Mordant in Zenker’s or Bouin’s before staining



+m Burned tissue

+n Burned tissue

o Haematoxylin expired

p Washes after haematoxylin, acid rinse and bluing steps exhausted

m Wash specimens more thoroughly

n Irretrievable.

o Change haematoxylin

p Use running water rinses, or change more frequently

3 Nuclear staining too dark

a Haematoxylin time too long

b Haematoxylin too strong

c Differentiating time too short

d Differentiating solution too weak

a Reduce time in haematoxylin

b Dilute haematoxylin Change to Gill or Mayer’s

c Increase time

d Use stronger (more acidic) solution

4 Nuclei reddish/purple

a Bluing ineffective

b Nuclei taking up eosin

a Increase time in bluing reagent

Check pH of bluing reagent aid change if necessary

b Increase time in differentiating step after counterstain

Lower the pH of the eosin

Change alcohols after counterstain

5 Brown nuclei a Over oxidised haematoxylin

a Use fresh haematoxylin

A78010402 Issue 470 A78010402 Issue 4 71



6 Nucleoli taking up haematoxylin

a Haematoxylin too concentrated

b Staining time too long

c Differentiating solution too weak

d Differentiating step too short

a Dilute haematoxylin

b Decrease time in haematoxylin

c Change solution

d Increase time

7 Artificially enlarged nucleoli

a Air drying a Irretrievable. Fix promptly

TABLE 3 - CYTOPLASMIC STAINING PROBLEMS (+ = H&E only; * = PAP only)


1 Counterstain too dark a Stain too strong

b Stain diluted with improper solvent

c Staining time too long

d Differentiating time too short

e Differentiating solution exhausted

f Differentiating solution not strong enough

g Stain warm, penetrates faster

a Dilute stain (use appropriate solvent)

Check percentage dye content and adjust formula if necessary

b Use correct solvent

c Decrease time

d Increase time

e Use fresh solution

f Use different solvent

g Adjust time accordingly

2 Counterstain too light +a Specimen in fixative too long

+-b Autopsy specimen

c Stain too weak

d pH of stain incorrect

a Increase staining time

b Increase staining time

c Do not dilute if intended for full strength use

Increase time in stain

Check percentage dye content and adjust if necessary

If exhausted, replace with fresh stain

d Adjust pH with glacial acetic acid

A78010402 Issue 472 A78010402 Issue 4 73

TABLE 3 - (Continuation) (+ = H&E only; * = PAP only)


e Differentiation too strong

f Expired stain

g Stain diluted with improper solvent

h Stain cold

e Dilute differentiating solution

Use different solvent

Decrease time

f Use fresh stain

g Use correct solvent

h Increase staining time

3 Cytoplasm is taking up haematoxylin

a High RNA content

b pH of haematoxylin too high

c Haematoxylin in too strong

a Increase differentiation time

b Readjust pH with glacial acetic acid

c Decrease time in haematoxylin

4 * Pseudoeosinophilia a Not enough light green

b Improper fixation

c Inflammation

d Inadequate differentiation after EA step

a Use EA-50 instead of EA-65

b Irretrievable

c Expected finding

d Increase time in alcohol Use fresh solution

5* Amphophilia a Poor levelling

b pH of EA incorrect

c EA expired

d EA exhausted

a Increase time in EA

b Check pH and adjust if necessary

c Use fresh EA

d Use fresh EA



6 Uneven cytoplasmic staining

a Poor levelling

b Solutions evaporated

+c Incomplete deparaffinisation

d Inadequate time

a Increase time in counterstain

b All solutions should cover specimen Replenish

c Change xylene

Deparaffinize longer

d Increase time in counterstain

7 Poor cytoplasmic transparency (cells appear dull, murky)

a Improper clearing

b Too much mounting media used

c Microscope not cleaned / aligned

d Stain expired

e Stain exhausted

f Incomplete deparaffinisation

*g Incomplete removal of carbowax

h Poor fixation

a Change absolute alcohol & xylene frequently to prevent contamination with water

Increase time in clearing agent

Level of xylene should exceed previous absolute alcohol which in turn should exceed previous 95%

+ Xylene used for deparaffinisation should not be used to clear

b Use minimal amount

c Clean microscope and use Kohler illumination

d Change counterstain

e Change counterstain

f Deparaffinize completely

g Soak slides for 10 min. in 95% alcohol prior to staining

h Irretrievable

A78010402 Issue 474 A78010402 Issue 4 75



i Differentiating solutions exhausted

j Inadequate dehydration

i Change alcohol rinses following counterstains frequently

j Dehydrate properly before counterstaining

8* No OG uptake a No keratinized cells present

b pH of OG-6 too high

c OG-6 exhausted

d OG-6 expired

e Solvent used to differentiate too strong

f Staining time too short

g Differentiation time too long

a None - expected finding

b Adjust pH with glacial acetic acid

c Change stain

d Change stain

e Use 95% ethanol

f Increase time

g Decrease time

9 Brown artifact (corn flakes)

a Xylene drying a Promptly coverslip slides after removal from xylene

10 Counterstain in nuclei

a Nuclear staining not adequate

b Cytoplasmic stain not differentiated completely

a Increase time in haematoxylin

Increase strength of hematoxylin

Decrease time in differentiating step

Increase strength of differentiating step

b Change differentiating solutions, use fresh

Increase time in differentiating solution

Use stronger solvent for differentiation

TABLE 4 - MISCELLANEOUS STAINING PROBLEMS (+ = H&E only; * = PAP only


1+ Blue haze surrounding sections

a Appears that albumin/gelatin adhesive stained by haematoxylin or haematoxylin affinity for glass

a Add acid rinse step

Increase time in acid differentiation step

Increase strength of acid solution (increase acid, decrease alcohol content)

Changed differentiating solution

2 Blue/black precipitate seen

a Haematoxylin has precipitated

a Filter haematoxylin

If precipitate will not filter out (Gill’s haematoxylin) change and use fresh

3+ Formalin pigment a Tissue fixed in nonbuffered formalin

a To remove pigment, immerse sections in saturated picric acid in 95% alcohol

To prevent pigment, use buffered formalin

4+ Sections yellow a Tissue stored in Bouin’s fixative (Picric acid)

a Place sections in a filtered saturated alcoholic solution of lithium carbonate until yellow is removed and proceed with hydration

5+ Sections falling off slide

a Not “baked” thoroughly

b No adhesive used

c Dirty slides

d Bluing reagent too harsh

a Increase time/temperature

b Add gelatin to waterbath or use albuminized slides

c Clean slides

d Use gentle bluing reagent

A78010402 Issue 476 A78010402 Issue 4 77



6 Floaters a Stains and reagents contain dislodged cells

a Change or filter solution

7 Halos seen a Microscope not aligned

b Mounting medium has incorrect refractive index

a Use Kohler illumination

b Change mounting medium

8* Shrunken cells a Inappropriate fixative used

a Use correct fixative

9 Xylene is cloudy (milky)

a Contaminated with water a Change xylene and absolute alcohols using clean dry dishes

b Change absolute alcohols more frequently

10 Water droplets seen a Contaminated clearing agent or mounting medium

a Use fresh solutions

11 Air bubbles a Air trapped during coverslipping

a If they can’t be “teased” out, remove coverslip and reapply, using minimal amount of mounting medium

Use thinner mounting medium

12 At completion of hydration, slides appear “bubbly”

a Hydration is incomplete a Return slides to water until runoff is smooth



13+Clear patches in sections after immersion in absolute alcohol, following deparaffinisation

a Deparaffinisation is incomplete

a Return slides to xylene and hydrate as usual

14 Slides appear opaque or cloudy after clearing

a Inadequate time in dehydrating solution

b Dehydrating solution contains water

a Increase time

b Use fresh solutions

15+ White patches in sections after hydration

a Water is trapped under paraffin, slide not dried & deparaffinized properly

a Place slides in absolute alcohol for several minutes, return to xylene to complete deparaffinisation and hydrate as usual

A78010402 Issue 478 A78010402 Issue 4 79

TABLE 5 - TROUBLESHOOTING HAEMATOXYLIN STAINING

SYMPTOM REMEDY

1 No Stain a Exhausted haematoxylin

b Excessive chlorine in tap water used for bluing or rinsing

2 Grey a Over-oxidation

b Over-processed specimen

3 Purple a Acidic Tap Water

b Too little time in bluing

c Exhausted bluing solution

4 Red to brown a Badly over-oxidised

b Long term exposure to air or sunlight

Resources - Thermo staff and Luna, L; Histologic Methods and Color Atlas of Special Stains and Tissue Artifacts, p. 70. 1992. Copyright American Histolabs Inc.

TABLE 6 - TROUBLESHOOTING PAPANICOLAOU STAINING

SYMPTOM REMEDY

1 Red nuclei a Increase time in bluing reagent

2 Nuclear staining too weak a Increase staining time

b Use higher strength haematoxylin

c Reduce concentration (not time) of acid alcohol

3 Background haematoxylin staining a Increase time in acid alcohol

4 Overall colour too blue a Increase time in acid alcohol

5 Cytoplasm too pink a Increase time in OG-6

6 Cytoplasm too orange a Increase time in alcohol after OG-6

7 Cytoplasm too blue a Increase time in EA-50

8 Cytoplasm too green a Increase time in alcohol after EA-50

It is important to remember that a number of minor modifications may be required to achieve the final desired result.

Alteration to the procedure should be done in a step-by-step process towards the desired goal. Start with the major problem and then build on that. For high quality results, it is important to ensure that all haematoxylin is removed from the cytoplasm. Therefore, a long treatment in acid alcohol is beneficial.

A78010402 Issue 480 A78010402 Issue 4 81

SPECIFICATION AND ACCESSORIES7.1 SPECIFICATION

7.1.1 Physical

Stainer Unit Width 720mm (281⁄2 ins) Depth 710mm (28 ins) Height 880mm (341⁄2 ins) Weight 80kg (176lbs)

Power Supply Unit Width 360mm (14 ins) Depth 463mm (181⁄4 ins) Height 145mm (61⁄2 ins) Weight 11kg (24lbs)

Monitor Standard PC compatible colour monitor

Weight Refer to manufacturer’s data

7.1.2 Electrical

Power Supply Voltages 100 - 240Vac (~)5A Frequency 50/60 Hz Power 150VA (non-heated version)/ 300VA (heated version) Monitor Voltage 100 - 240Vac (~)3A Frequency 50/60 Hz

7.1.3 Fuses

Thermo Part NumberMains plug fuse 13A 250V Mains input fuses Non-heated T5A A78010465x2 Heated T10A

FUSES SHOULD ONLY BE REPLACED BY TECHNICALLY COMPETENT PERSONNEL.

7.1.4 Switch Convention

Power On IPower Off O

7.1.5 Water Supply

Minimum 0.2 millibars (3psi)Maximum 10.0 millibars (145psi)

If water pressure is more than 10 millibars, install a pressure-reducing valve.

7.1.6 Environment

General Indoor use onlyTemperature +5°C to +40°C(operation)

Temperature -25°C to +55°C(transit/storage)

Humidity 80% max. for temperatures < 31°C 50% max. for temperatures 31°C to 40°C (Non-condensing environment)

7.1.7 Shandon Varistain Gemini Automatic Stainer Part Numbers

No Heat Dual Voltage No Monitor A78000001 With Monitor A78000002

With Heat Dual Voltage No Monitor A78000011 With monitor A78000012

A78010402 Issue 482 A78010402 Issue 4 83

7.2 ACCESSORIES THERMO PART NUMBER

Baskets/Carriers (pack of 5) (each holds 20 slides) A78010466

Basket Adaptor for large slides (pack of 10) A78010510Slide size - up to 75 x 58mm (3” x 21⁄4”)

Carrier for Sakura basket (pack of 5) A78010404

Consul Clip Transfer Tool A78010401

Consul Clip Rod and extension kit A74110291

Charcoal Filter 9990610

Reagent Troughs (pack of 3) A78010487(Each trough volume = 320ml)

Reagent Trough covers - large (pack of 4) A78010488

Reagent Trough Covers - single trough (pack of 6) A78010489

Vent Kit A78010467

VGA Monitor AP14172

VGA Extension Cable AP14185

Water Trough A78010201

Water Wash Inlet Hose AP14160

PRODUCT RETURN SAFETY DECLARATION

Thermo Electron Corporation, 93-96 Chadwick Road, Astmoor, Runcorn, Cheshire, WA7 1PR, United KingdomTel: +44 (0) 1928 566611; Fax: +44 (0) 1928 565845; www.thermo.com/shandon

Part 1 DECONTAMINATION CERTIFICATE

Any instrument or part of any instrument must be clean before being returned, and where necessary accompanied by a completed Decontamination Certificate. Should the instrument or any part of it be received in an unclean condition, or Thermo Electron Corporation consider it to be a hazard, the instrument or part will be returned unrepaired at the expense of the customer.

It is important that the certificate is forwarded by post or fax, and a copy attached to the exterior of the container. Containers will not be opened until the company is in possession of the required certificate.

This form MUST be completed by the customer and NOT a Thermo or distributor employee.

If an instrument or part is to be returned to THERMO, please note the following:-

1 If the instrument or any part of it has been exposed to, or been in contact with potential pathogenic or radioactive material, it is essential that it is decontaminated.

2 Set procedures are laid down in the European Health and Safety Directives for decontamination. To avoid any misunderstanding, we request that all instruments or parts returned to us must be accompanied by a certificate stating the following:

We certify that this (Model) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Serial No

· has not been exposed to pathogenic, radioactive or other hazardous material and has been cleaned

OR

· has been decontaminated and cleaned (if exposed to the above) according to approved procedures, following exposure to:

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Signed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Position . . . . . . . . . . . . . . . . . . . . . . . . .

Name (Block Capitals) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Company or Organisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Full address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

· Has the instrument been used for work with human, or animal, Transmissible Spongiform Encephalopathies, e.g. Creutzfeld-Jacob disease, Scrapie or BSE? YES / NO

If yes, please contact Thermo Service before taking any further action.

Part 2 Guidelines for Returning Instruments

Please use the checklist below to ensure that the instrument being returned is ready for collection.

· All reagents / wax removed from instrument, including vapour traps (if applicable). . . . . . . . . . . . . . . . .

· Accessories are secured / itemised . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

· Instrument has had transit clamps fitted as per operator guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

· Instrument is packed in original packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . YES / NO

RMA NUMBER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

CARRIER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

FOR ATTENTION OF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

A78010402 Issue 484 A78010402 Issue 4 85

WARRANTY STATEMENT

Thermo is proud of its quality and reliability, and of its after-sales service. Thermo continuously strives to improve its service to its customers.

Ask your distributor or representative about Service Contracts, which can keep your purchase in peak condition for many years to come.

Warranty provisions necessarily vary to comply with differences in national and regional legislation, and you can find details in your delivery documents, or from your dealer or representative.

Note that your warranty may be invalidated if:

• The instrument is modified in any way

• Accessories and reagents are used that are not approved by Thermo

• The instrument is not operated or maintained in accordance with the instructions in this Operator Guide

Issued by: K Waldron Quality Manager Thermo Electron Corporation Anatomical Pathology, Clinical Diagnostics

This product conforms with the essential requirements of the following directives:

In Vitro Diagnostics Directive 98/79/EC

Low Voltage Directive 73/23/EEC (as amended by 93/68/EEC)

This product complies with the following International Standards:

EMC: EN61326 EN61000-3-2 EN61000-3-3

Safety: IEC1010-1 - non-heated version IEC1010-2-010 - heated version only CAN / CSA - C22.2 No 1010.1-92

UL Std No. 3101

Declaration of ConformityThis Declaration of Conformity is only valid when the instrument is used in accordance with this Operator Manual

Manufacturer’s Name: Thermo Electron Corporation

Manufacturer’s Address: Chadwick Road, Astmoor, Runcorn, Cheshire, WA7 1PR ENGLAND

Product Description: Automatic Stainer (heated or non-heated)

Product Designation: Shandon Varistain Gemini (A78000001, A78000002,

A78000011, A78000012) including accessories supplied as standard

Year of Marking (CE): 2000

Date: 23 September 2003

Optional accessories considered subject to the In Vitro Diagnostic Directive (IVDD) are specifically identified on this Declaration of Conformity. Further supplies of standard accessories are treated as spares. Convenience aids offered as accessories are not subject to the IVDD

A78010402 Issue 486 A78010402 Issue 4 87

APPENDIX A TRANSPORTATION INSTRUCTIONSA.1 INTRODUCTION

If you ever need to transport the Shandon Varistain Gemini stainer, the following packaging instructions should be followed.

A.2 TO REPACKAGE THE STAINER UNIT

Note

1 If the Shandon Varistain Gemini stainer is to be serviced or returned to Thermo, it must be cleaned and decontaminated, and the Product Return Safety Declaration (see page 83) signed.

Make sure that all the baskets have been removed from the instrument and that the hook is in the left hand hole in the upper deck (as viewed from the front of the instrument)

Press the O (OFF) side of the mains power switch (a) to switch the mains power switch off.

Wait for about 30 seconds while the instrument shuts down, and then press the O (OFF) side of the Battery Isolation Switch (b).

Disconnect the mains cable from the mains supply and the Power Supply Unit.

Disconnect all cables between the Power Supply Unit, the Shandon Varistain Gemini stainer and the monitor (c).


Nominal Ratings:

��

A78010351 Issue 10PS0001E0001

��

��

�

� ��

c

a

l0

b

Repackage the monitor in accordance with the manufacturer’s instructions.

Turn off the water supply and disconnect the blue inlet hose at the right hand rear corner. Place the inlet hose (AP14160) and Jubilee clip (P12026) into a bag.

Empty and dry the water wash troughs. Empty out as much water as possible from the black drain hose. Coil the hose at the rear of the instrument and use cable ties at the two fixing points (a) to fix the coil to the back panel of the instrument.

Gently press on the hook to lower it by approximately 25 - 50mm. Carefully fit the Hook Support foam piece A to the hook from the top. This grips the hook and top deck to prevent any movement of the mechanism.

Place a basket and carrier into pots 4, 5, 6, 15, 16, 17 on the lower deck and W2, W3, W4 and W5 on the upper deck (10 in total).

Place the four multi-trough lids (A78030215) in two stacks on top of the lower level of troughs.

Place the two semi-circular cardboard pieces D over the pots - four on each level, overlapped by 90° as shown.

a

A

D

A78010402 Issue 488 A78010402 Issue 4 89

Place the two foam pieces B between the cardboard on the top deck and the top cover. These should be pushed into place to keep the cardboard pieces D in position.Place the two shorter foam pieces C between the cardboard on the lower deck and the underside of the top deck at the water wash positions. Again, these should be pushed into place to keep the cardboard pieces D in position.

Gently push the foam piece E between the upper sliding door and upper layer of pots.

Gently push the smaller foam piece F between the lower sliding door and the turntable.

Place three spare troughs into a bag. (Place them in line as shown in the diagram (a)).

Place six small trough lids (A78030142) into two polythene bags (three in each), and place in the bag with the troughs.

Tie wrap the bag containing the three troughs and trough lids to the lower cardboard ‘C’ piece inside the unit.

Close the large doors and ensure the sliding doors are in the central position.

a

E

F

B

C

D

Place the bag containing the inlet hose at the rear of the instrument. Cling film the outside of the unit using packaging film - three full circuits around the unit starting at the top front and finishing at the bottom.

THE SHANDON VARISTAIN GEMINI STAINER IS HEAVY, GET HELP TO LIFT IT. HOLD THE STAINER SECURELY AT THE LIFTING POSITIONS LOCATED UNDER THE SIDES OF THE INSTRUMENT (b).

Place the stainer onto the skid base so that the unit sits in the foam perimeter (c).

Note

1 The front of the instrument is indicated on the skid base.

Place the foam over-shipper over the unit (d).

Place the cardboard over-shipper over the foam over-shipper (e).

Seal and staple box and place two bands around to secure the box to the skid. The stainer is now ready to be transported.

b b

d

e

c

A78010402 Issue 490 A78010402 Issue 4 91

A.3 TO REPACKAGE THE POWER SUPPLY UNIT

Get help to lift the Power Supply Unit if necessary. Hold the unit securely at the lifting positions located under the sides of the unit (a).

The Battery Isolation Switch (b) must be switched to the OFF position (O on Battery Isolation Switch pushed inward).

Fit the two side foam pieces (c) to either side of the power supply and carefully place in the packing box (d).

Place the manual in a polythene bag on top of PSU.

Put each mains lead into a polythene bag (six mains leads - 2 x UK (AP14202); 2 x USA (P12032); 2 x Europe (P12822)). Place these into the spaces in the PSU pack (e).

Put the data lead (A78030174) and monitor extension lead (AP14185) separate polythene bags. Place these into the spaces in the PSU pack (e).

Close, staple and tape box. The Power Supply Unit is now ready to be transported.


Nominal Ratings:

��

A78010351 Issue 10PS0001E0001

��

��

�

� ��

b

c

d

e

a

APPENDIX B APPROVED REAGENT LISTB.1 INTRODUCTION

Refer to ‘Safety and Chemicals’ section on page 7 before the instrument is filled with reagents.

This Section lists all the reagents that Thermo specify can be used with the Shandon Varistain Gemini stainer.

If you want to use a reagent not included in this list, contact your Thermo agent for advice.

B.2 REAGENTS

XyleneTolueneShandon Xylene Substitute (Shandon Histosolve)Industrial Methylated Spirits (IMS) (up to 5% methanol in ethanol)EthanolIsopropanol (IPA)Acid Alcohol (1% HCI in 70% Ethanol)Acid Alcohol (10% Glacial Acetic Acid in 70% Ethanol)Eosin (Aqueous and Alcohol based)HaematoxylinEA50/65OG6Phosphotungstic acid (1% in water)Ammonia Water (1% Ammonium Hydroxide in water)Scotts Bluing ReagentSodium Hypochlorite (10% in water)WaterSchiff’s ReagentPeriodic Acid

WARNING

A78010402 Issue 492 A78010402 Issue 4 93

PO

ST-

STA

INP

RE

-STA

INS

TAIN

APPENDIX C EXAMPLE STAINING PROCEDURES

C.1 INTRODUCTION

These staining protocols are provided for convenience only - you may need to modify them to suit your reagents, water and other local conditions. Thermo has not validated the protocols illustrated here, and takes no responsibility for their use. Customers should always validate protocols before placing any reliance on them.

C.2 HAEMATOXYLIN AND EOSIN STAINING ROUTINE

STEP REAGENT CONC. % TIME (mins:secs)

1 Xylene 2:00

2 Xylene 1:00

3 Xylene 1:00

4 Alcohol (IMS) 100.0 1:00

5 Alcohol (IMS) 100.0 1:00

6 Alcohol (IMS) 100.0 1:00

7 Water Wash 1:00

8 Haematoxylin 4:00

9 Water Wash 1:00

10 Acid Alcohol 0:15

11 Water Wash 1:00

12 Bluing Reagent 1:00

13 Water Wash 1:00

14 Aqueous Eosin 1:00

15 Water Wash 1:00

16 Alcohol (IMS) 100.0 1:00

17 Alcohol (IMS) 100.0 1:00

18 Alcohol (IMS) 100.0 1:00

19 Xylene 1:00

20 Xylene 1:00

21 Xylene 1:00

STA

INP

OS

T-S

TAIN

PR

E-S

TAIN

C.3 PAPANICOLAOU (PAP) STAINING ROUTINE


1 Alcohol (IMS) 95.0 1:00



4 Water Wash 1:00

5 Haematoxylin 4:00

6 Water Wash 1:00

7 Acid Alcohol 0:15

8 Water Wash 1:00


10 Water Wash 1:00

11 Alcohol (IMS) 70.0 1:00

12 Alcohol (IMS) 95.0 1:00

13 OG6 2:00

14 Alcohol (IMS) 95.0 1:00

15 Alcohol (IMS) 95.0 1:00

16 EA50 3:00

17 Alcohol (IMS) 95.0 1:00

18 Alcohol (IMS) 100.0 1:00

19 Alcohol (IMS) 100.0 1:00

20 Alcohol (IMS) 100.0 1:00

21 Xylene 1:00

22 Xylene 1:00

23 Xylene 1:00

A78010402 Issue 494 A78010402 Issue 4 95

STA

INP

OS

T-S

TAIN

PR

E-S

TAIN

C.4 PERIODIC ACID SCHIFF (PAS) STAINING ROUTINE


1 Xylene 2:00

2 Xylene 1:00

3 Xylene 1:00

4 Alcohol (IMS) 100.0 1:00

5 Alcohol (IMS) 100.0 1:00

6 Alcohol (IMS) 100.0 1:00

7 Water Wash 1:00

8 Periodic Acid 5:00

9 Water Wash 1:00

10 Schiff’s Reagent 5:00

11 Water Wash 5:00

12 Haematoxylin 2:00

13 Water Wash 1:00


15 Water Wash 1:00

16 Alcohol (IMS) 100.0 1:00

17 Alcohol (IMS) 100.0 1:00

18 Alcohol (IMS) 100.0 1:00

19 Xylene 1:00

20 Xylene 1:00

21 Xylene 1:00

APPENDIX D TO PERFORM A MEMORY RESET

D.1 INTRODUCTION

Follow these instructions to perform a memory reset if the sequence for turning the instrument off has not been carried out correctly.

ALL OPERATIONAL STATUS INFORMATION WILL BE LOST.

D.2 INSTRUCTIONS

1 Switch the instrument on by following the instructions in Section 2.9.

2 When the language select screen is displayed, press [ ].

3 When the Help screen is displayed, press [7].

4 When the Help screen disappears, a full memory reset will have been carried out.

5 Reset the clock (see paragraph 4.3.1).

6 Reload the instrument setup from disk (paragraph 4.3.5), or follow the instructions in Section 4.2 to program the instrument.

A78010402 Issue 496 A78010402 Issue 4 97

A

Accessory InstructionsConsul® Clip Transfer Tool 21Vent Kit 23

Access CodesTo change an access code 61To clear an access code 62To set an access code 61To use the access code 62

APPROVED REAGENT LIST 91Introduction 91Reagents 91

Audio Configuration Screen[CONTINUOUS] 59[DEMONSTRATE] 59[NEW TUNE] 59[SILENCE] 59

B

Batch Monitoring Screen 52[AUTO RETURN] 54[STOP] 54

Battery Charging 20Battery Disposal 6Battery Isolation Switch 19, 86, 90Battery Type 7

C

CLEANING AND MAINTENANCECleaningCleaning the Power Supply Unit 64Cleaning the Stainer 63Washing Procedures 64

Maintenance 64Connecting the system 16

To Connect a Monitor to the Stainer 16To Connect a Printer 17Recommended Printer Types 17

Consul® Clip Transfer Tool 10, 21CONTROLS 25

Description 25Main Control Panel 25[*] 27[CANCEL] 27[ENTER] 26[EXIT] 27Direction Arrow Keys 27Function Buttons 25Help Key 27

Number Keys 26Silence Alarm 27Standby Key 27

D

DATA MANAGEMENT 58, 60, 61[DISC DRIVE] 60, 61[PRINTOUTS] 60, 61

Declaration of Conformity 85Define Procedure Screen 35

[CHANGE REAGENT] 36[DELETE STEP] 35[INSERT STEP] 35Define Reagent Names 32

DESCRIPTION 9Overview 9

Disc Drive Options Screen 61[START] 61

E

Earthing Requirements 18Electrical Requirements 18EXAMPLE STAINING PROCEDURES 92

HAEMATOXYLIN AND EOSIN STAINING ROUTINE 92

INTRODUCTION 92PAPANICOLAOU (PAP) STAINING ROU-

TINE 93PERIODIC ACID SCHIFF (PAS) STAINING

ROUTINE 94

F

Filter, To Fit 14

H

Heater Station Troughs - to remove and replace 31

How to Stain 31, 39, 49

I

INSTALLATION AND SETTING UP 11Accessories 21Consul® Clip Transfer Tool 21Large Slide Basket Adaptor 24Vent Kit 23

Electrical Requirements 18Battery Isolation Switch 19, 86, 90

On/Off Switch 18Wiring Convention 18

Procedure 11Overall Dimensions - PSU 12Overall Dimensions - Stainer 11Siting Requirements 11Water Supply Position 11

To Adjust the Turntable 15To Connect the System 16To Connect a Monitor 16To Connect a Printer

Recommended Printer Types 17

To Fit the Filter 14To Level the Instrument 13To Adjust the Levelling Feet 13

To Switch on and off 19To Switch On 20

To Unpack 12Lifting the Power Supply Unit 12Lifting the Stainer 12, 13Positioning the Monitor 13

K

keyboard 31, 34Keyboard Screens 31

[CAPS LOCK] 32[COMPLETE] 32[CONTROL] 32

L

language 20, 30, 95Language, To Select 20lifting position 89, 90Lifting Positions 89loading of reagents 15, 51Loading Reagents 51Loading Slides Screen 55

[SET AS DEFAULT] 56[STANDARD START] 55[URGENT START] 55

M

Main Menu Screen 26, 29, 30, 37, 58, 60, 61[MONITORING] 26[OPTIONS] 26[PROGRAM] 26[STAIN] 26

Material Safety Data Sheets (MSDSs) 2Memory Reset 19memory reset 19, 95

O

On / Off 59On / Off Switch 59OPERATION 29

Housekeeping Tasks 58Quality Control Settings 58To Change the Time and Date 58To Save or Retrieve Information From a

Floppy Disk 60How to Stain 29Introduction 29Define the Reagents to be Used 30Define the Staining Procedures to be Used

32Quality Control Warning Screen 57Reagent Layout 1 Screen 39Reagent Layout 2 Screen 42

Heater Sharing Control Screen 47

Reagent Sharing Control Screen 46

Water Wash Sharing Control Screen 47

Select the Stain Program 30

P

Printer 60Printout Options Screen 60

[DISC DRIVE] 60[ON-SCREEN] 60[ PRINTER] 60

Procedure Select Screen 33[CHANGE NAME] 34[CHANGE NOTES] 34[REMOVE] 33[RETAIN] 33[SELECT] 33

Product Return Safety Declaration 83

Q

Quality Control Screen 59[ON / OFF] 59

Quality Control Warning Screen 51, 53, 57

R

Reagent Layout 1 Screen 33, 39, 42, 45, 47, 56[ADD PROCEDURE] 39[DELETE PROCEDURE] 39[ REAGENT LAYOUT 2] 39

Reagent Layout 2 Screen 39, 42[ADD PROCEDURE] 45[CHANGE GROUPS] 45[CHANGE OPTIONS] 47[COMPLETE] 47

INDEX

A78010402 Issue 498

[DELETE] 47[DUPLICATE] 48[MOVE] 48[OPTIMISE] 48[AUTO ALLOCATE] 48[AUTO ARRANGE] 48

[PRINT] 48Reagent List 91Reagent Monitoring Screen 49

[BATCH MONITORING] 52[CONFIRM LOADED] 51

S

Safety and Chemicals 7, 49, 91Setting Up 11Set Clock 58

[12 / 24 Hour Clock] 58[DAY <-> MONTH] 58

SPECIFICATION AND ACCESSORIES 80Specification 80Electrical 80Fuses 80Physical 80Switch Convention 80Water Supply 80

Staining 8, 9, 10, 26, 28, 29, 31, 32, 35, 39, 43, 44, 49, 50, 51, 53, 54, 57, 58, 59, 64, 65, 67, 68, 69, 71, 72, 73, 74, 79

SYMBOLSBiohazard 2Harmful chemicals 2High temperatures 2Safe operation 2Warning 2

Symbols and Conventions 2

T

TRANSPORTATION INSTRUCTIONS 86Introduction 86To Repackage the PSU 90To Repackage the Stainer 86

TROUBLE SHOOTING 65CYTOPLASMIC STAINING PROBLEMS

71, 72EQUIPMENT STATUS CHECKS 66Introduction 65NUCLEAR STAINING PROBLEMS 67

U

Unload 28, 41, 42, 55Unpacking 11

V

Vent Kit 14, 23

W

warnings 51, 53, 57, 58WARRANTY STATEMENT 84Water Supply and Drainage 14WELCOME 5

Introduction 5Safety 5Safety and Chemicals 7

shandon varistain gemini - thermo fisher scientific a78010402... · xiv 31⁄2” floppy disk drive...

Documents