SET 2

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QUESTION PAPER FOR THE POST OF LABORATORY ASSISTANT

1. Blood culture is indicated in the following bacterial diseases, except:a) Meningitisb) Endocarditisc) Gastroenteritisd) Pyelonephritis

2. NABL stands fora) National Accreditation Bureau of laboratoriesb) National Authorities of board of laboratoriesc) National Authority o f Botanical laboratoriesd) National Accreditation Board for testing and calibration laboratories

3. Which o f the following is an indication of Red Cell Distribution Width (RDW)?a) Variability o f RBC volumeb) Poikilocytosisc) Macrocytosisd) Microcytosis

4. Group A beta-hemolytic streptococci are best characterized by which o f the following:a) Positive cAMP testb) Optochin sensitivityc) Bile esculin-hydrolysisd) Bacitracin sensitivity

5. Which of the following organisms in most frequently associated with endocarditis?a) Virusesb) Neisseriac) Staphlyococcid) Streptococci

6. Which is responsible for the process o f cell division?a) Mitosisb) Heterosisc) Fusiond) Fission

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SET 27. Non-clotting o f blood is caused by deficiency of?

a) Vitamin Ab) Vitamin Cc) Vitamin Ed) Vitamin K

8. Zika virus was first isolated in 1947 from the forests of?a) Ugandab) Nigeriac) Congod) Kenya

9. The most likely cause o f an elevated potassium level in an apparently normal individual is:a) Contaminationb) Hemolysisc) Acute renal failured) Interfering substances

10. Which of the following method is used to measure ionized calcium?a) Flame photometryb) Colour complex formation between calcium and o-cresolphthaleinc) Atomic absorptiond) Calcium ion selective electrodes

11. The recall rate is also known as:a) Sensitivityb) Specificityc) Aliquotd) Circardian rhythm

12. If a compound has a pH of 6.5, it has a pOH of:a) 6.5b) 7.5c) 3.16x1 O'7d) 3.16xl0'8

13. The liquid vapour pressure is increased when:a) The pressure is loweredb) The temperature is loweredc) The temperature is raisedd) None of these

14. A calorimeter is used to:a) Determine the heat o f a reactionb) Determine the heat given off/absorbed during some processc) Store the heat from a chemical reaction.d) None of these

15. Proteins may be estimated by the following methods, except:a) Biuret methodb) Heat coagulationc) Kjeldahl’s digestiond) Nephelometry

SET 216. The urine of patient with homocystinuria will be positive for;

a) Benedict's testb) Ferric chloride testc) Rothera’s testd) Cyanide nitroprusside test

17. Bilirubin in serum can be measured by:a) Van den Bergh reactionb) Ehrlich’s reactionc) Schlesinger’s reactiond) Fouchet’s reaction

18. All the following manifestations are seen in sickle cell anaemia (HbS disease), except:a) Pain and swelling in jointsb) Hemolytic anemiac) Sickle cells in peripheral circulationd) Inclusion bodies in RBCs

19. Which phenomenon is not related to functional properties of hemoglobin?a) Bohr effectb) Haldane effectc) Pasteur effectd) Donnan effect

20. Alkaline phosphatase level in serum is elevated in all the following conditions, except:a) Obstructive jaundiceb) Ricketsc) Infective hepatitisd) Myocardial infarction

21. The following are lipolytic hormones, except:a) Glucagonb) Cortisolc) Epinephrined) Insulin

22. Which laboratory finding is abnormal in a pregnant women at 28 weeks gestationa) Serum alkaline phosphatase level o f 25 KAU/dlb) Plasma 1 hr post-prandial glucose o f 200 mg/dlc) Alpha fetoprotein level o f 120 ng/mld) Plasma triglyceride level o f 150mg/dl

23. In the enzymatic method of glucose estimation, the following reactants are used, except:a) Glucose oxidaseb) NAD+c) Peroxidased) Hydrogen peroxide

24. Benedict's reagent will be reduced by all the following substances seen in urine, except:a) Fructoseb) Galactosec) Pentosesd) Sucrose

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SET 225. Which of the following laboratory results indicate poor glycemic control?

a) PPBS of 180 mg/dlb) Serum cholesterol o f 240 mg/dlc) Glycated haemoglobin o f 10%d) Blood urea level of 35 mg/dl

26. Proteinuria is seen in all the following conditions, except:a) Acute glomerulonephritisb) Diabetes insipidusc) Pyelonephritisd) Multiple myeloma

27. Best time for collection o f blood for malaria:a) Before and after paroxysmb) Shortly after paroxysmc) Later paroxysmd) Just before paroxysm

28. Some organisms are said to be pathogenic if they are containing one o f the following features:a) Coagulaseb) Catalasec) Sugard) Antibodies

29. One is always non motile gram - ve rods:a) Haemophilus Influenzab) Shigellac) Rcolid) Salmonella

30. What is the confirmatory test o f streptococcus pneumonia:a) Catalaseb) Optochine discc) Coagulased) Bile insolubility

31. All the following biochemical parameters are indices of liver function, except:a) Bilirubinb) Cholesterolc) Albumind) Creatinine

32. GLP stands for :a) Gross Lethal Powerb) Good Laboratory Practicesc) General Laboratory Proceduresd) None of the above

33. For complete blood count sample is collected in ................. Vaccutainera) Yellow Topb) Green Topc) Purple Topd) Black Top

SET 234. Normal blood cholesterol level is:

a) 40-60 mg/100 mlb) 70-110 mg/100 mlc) 120-150 mg/100 mld) 150-200 mg/100 ml

35. Normal value of blood urea is:a) 3-4 mg/100 mlb) 4-8 m g/100 mlc) 8-16 mg/100 mld) 20-40 m g/100 ml

36. All of the following are requisites for PCR, except:a) DNA primerb) Taq polymerasec) Restriction endonucleased) Deoxynucleotide triphosphates

37. The coding unit of a DNA is called as:a) Intronb) Exonc) Cistrond) Prion

38. In agarose gel electrophoresis, DNA is moved towards:a) Cathodeb) Anodec) DNA doesn't moved) None of above

39. Normal level of calcium in blood is:a) 3-4 mg/dlb) 9-11 mg/dlc) 4-5 mEq/dld) 96-106 mEq/dl

40. The hospital department that studies alcohol, drugs, poisons and heavy metals isa) Serology/Immunologyb) Toxicologyc) Cytologyd) Endocrinology

41. If a haemoglobin electrophoresis result is HbAl or HbA2, it means the patient hasa) Sickle cell anaemiab) Foetal haemoglobinc) Normal haemoglobind) Haemolytic anaemia

42. Which of the following Biomedical wastes cannot be disposed off in Yellow Bags?a) Reactive chemical wastesb) Human anatomical wastesc) Microbiology and Biotechnology wastesd) Dressings soiled with blood

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SET 243. Medium commonly used in blood bank for safe storage of blood is ...........

a) Citrate Phosphate Dextrose adenineb) EDTA anticoagulantc) Heparin anticoagulantd) Saline alardine glucose maltose (SAGM)

44. Indirect anti- antibody test used to detect (Indirect Coombs Test)a) Sensitized RBCs in patient bloodb) IgGc) IgMd) Sensitized antibody in patient serum

45. The nitrogenous base present in lecithin isa) cholineb) ethanolaminec) inositold) serine

46. Life span of red blood cells in the circulation (Body)a) 180 daysb) 120 daysc) 130 daysd) 20 days

47. Out of various free radical species, the following radical is most reactivea) Superoxide (O2')b) Hydrogen peroxide (H2O2)c) Nitric oxide (NO)d) Hydroxy (OH')

48. Antigen antibody complex area) Weakly boundb) No boundsc) Strongly boundd) All

49. The Enzyme in ELISA testing is present in thea) Bufferb) Micro platec) Conjugated) All

50. A standard micro plates in ELISA test hasa) 98 wellsb) 94 wellsc) 96 wellsd) None

51. Poliomyelitis is transmitted througha) Skinb) Respirationc) Faeco-orald) All

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SET 212. A parasite which does not deposit eggs in the intestine

a) E. vermicularisb) A. duodenalec) A. lumbricoidesd) All

53. HIV contaminated waste products can be decontaminated by the following agents excepta) Sodium hypochloriteb) Formaldehydec) Methanold) Glutaraldehyde

54. Infective stage ofAscaris lumbricoidesa) Larvab) Cercariac) Eggd) Cyst

55. The worm that causes blood in urinea) Ascarisb) Fasciolac) Schistosoma haematobiumd) None

56. Iodine stain o f Entamoeba cyst used to detecta) Chromatoidal barsb) Cell wallc) Nucleid) All

57. Intracellular parasitea) E. colib) Toxoplasma gondiic) Trichomonas vaginalisd) None

58. Alkaline phosphatase is the important enzyme to detect function in i Liver, ii Bone, iii Braina) i & iiib) ii & iiic) i & iid) i, ii & iii

59. Direct anti- antibody test used to detect (Direct Coombs Test)a) Sensitized RBCs in patient bloodb) IgGc) IgMd) Sensitized antibody in patient serum

60. The bonds in protein structure that are not broken on denaturationa) Hydrogen bondsb) Peptide bondsc) Ionic bondd) Disulfide bonds

SET 261. The best sample for the culture of children poliomyelitis is

a) Anal Swabb) Blood Culturec) Stool cultured) All

62. The most abundant source o f ATP in cell isa) Mitochondriab) Cytoplasmc) Nucleusd) Cell wall

63. Organism Soluble in Bilea) Staphylococcusb) Streptococcusc) Pneumococcusd) Haemophilous influenza

64. Reticulocyte is immaturea) RBCb) WBCc) Plateletd) All

65. Leukocyte responsible for cellular immunitya) T- lymphocyteb) B- lymphocytec) Monocyted) Basophil

66. Malaria infection is transmitted bya) Male anopheles mosquitob) Female anopheles mosquitoc) Bothd) None

67. Detection of malaria parasite is bya) Thin Blood filmb) Thick blood filmc) Bothd) None

68. Neutrophil count is high ina) Acute bacterial infectionb) Iron deficiency anemiac) Megaloblastic anemiad) All

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SET 269. Purpose of standard deviation

i. To measure external quality controlii. To measure internal quality controliii. Precision and accuracya) i & iiib) ii & iiic) i & iid) i, ii & iii

70. The (U) unit is used for evaluation ofa) Protein in serumb) Haemoglobinc) Hormone Assayd) Enzymatic Activity

71. The reaction given by two or more peptide linkages isa) Biuret testb) Ninhydrin testc) Xanthoproteic reactiond) Pauley's test

72. The nitrogenous base not present in DNA structurea) Adenineb) Guaninec) Cytosined) Uracil

73. Which one o f the vitamin A functions is as a steroid hormonea) Retinalb) Retinolc) Provitamin Ad) beta- carotene

74. The immunoglobin present in most abundant quantitya) IgGb) IgAc) IgMd) IgE

75. Name the immunoglobulin involved in body allergic reactionsa) IgAb) IgEc) IgDd) IgM

76. The following anticoagulant binds with Ca2+ and prevents blood clottinga) Heparinb) Oxalatec) Proteind) All of them

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SET 277. Inner mitochondrial membrane is impermeable to

a) H+b) K+c) OH-d) All

78. Amyloid is stained bya) Congo Redb) Eosinc) Hematozylind) Alizarin Red

79. Prussian blue is used for staininga) Haemosiderinb) Bonec) Muscled) Fat

80. Punctate basophilia are seen in poisoning witha) Leadb) Calciumc) Sodiumd) Potassium

81. The chemical nature o f the primer required for the synthesis of DNAa) DNAb) Histonec) RNAd) None o f the above

82. Psammoma bodies are seen ina) Hodgkins lymphomab) Carcinoma thyroidc) Bothd) None

83. Incubation Period of Hepatitis B isa) Unknownb) 4 to 26 weeksc) 2 to 8 weeksd) 1 to 6 weeks

84. Locomotion along chemical gradient is calleda) Diapedesisb) Chemotaxisc) Bothd) None

85. Pappenheimer bodies are seen ina) Sideroblastsb) Late normoblastsc) Intermediate normoblastsd) Siderocytes

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SET 286. Most specific antibody for Systemic lupus erythematosus (SLE)

a) Anti dsDNAb) SS-A(Ro)c) SS-B (La)d) Anticentromere

87. For counting CD4+ T cells in AIDS the following technique is often employeda) In-situ hybridisationb) Flow cytometryc) Polymerase chain reactiond) Electron microscopy

88. Part of the respiratory tree not lined by pseudostatified epitheliuma) Bronchiolesb) Larynxc) Vocal Cordsd) Trachea

89. Which is not a feature o f apoptosisa) Nuclear compactionb) Cellular swellingc) Cytoplasmic eosinophiliad) Intact Cell membrane

90. A child diagnosis show scarlet fever the cause isa) Streptococcus pyogenesb) Staphylococci aureusc) Bothd) None

91. Oxygen haemoglobin dissociation curve is.................a) J Shapedb) Flatc) Obliqued) Sigmoid

92. Bacteria arranged in Chinese letter patterna) Corynebacteria diphtheriab) Bacillus anthracisc) Bothd) None

93. Factor VII deficiency can be diagnosed bya) Bleeding timeb) Prothrombin timec) Clotting timed) Platelet count

94. Principle for colorimetric test is................a) Two Point reactionb) End point reactionc) Ascending order reactiond) Descending order reaction

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SET 295. Naturally occurring antibodies in the serum of a non-transfused person are:

a) IgAb) IgDc) IgGd) IgM

96. The best media for urine culture isa) Blood agarb) Chocolate agarc) CLEDAgard) All

97. The following antibody stain is used in immunohistochemistry to identify epithelial cellsa) Desminb) Vimentinc) Cytokeratind) Neurofilaments

98. False Negative reaction in R A factor test is due toa) Raynaud’s phenomenonb) Prozone phenomenonc) Gilbert’s phenomenond) All of the above

99. Nutrient agar isa) Selective mediab) Differential mediac) Special mediad) Basic Medium

100. Specimen suitable for microfilariaa) Tissueb) Bloodc) Fluidd) All

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