scientific talks in general and srip in particular
TRANSCRIPT
Scientific Talks
in general and
SRIP
in particular.
The Ten Minute Rule
No matter how much time has been allottedImagine that you have only 10 minutes
This will force you to isolate the important information
Distill your data down to one point
Then state the significance in one sentence
This is the THM
Step 1 :
Isolate the Take Home Message (THM)
Mike Tyson vs Evander Holyfield
Janet Jackson and Justin Timberlake
Don’t Drown In Your Data
Months later, nobody will remember the details!
For example what was memorable about:
What color were her shoes?
What round?
Your data is complex
But there is one veryimportant piece of data
Isolate it!
Step 2 The Introduction
Purpose- Start from nothing and get to the THM
Remember:
Who’s your Daddy? (your audience)
Just the Facts (Don’t show off)
•Determine the “main question” or hypothesis
•Very Specific
•The answer will have broad implications and be the THM
Remember:
Who’s your Daddy?
Just the Facts
Introduction
(The question)
Conclusion
Results
(The data and interpretation)
(The THM )
Q. How much data?
Q. How many slides?
A. = T/2(rule of thumb)
For a 10 minute talk, 5 slidesFor a 30 minute talk, 15 slidesetc
The main question dictates the data you present
Data orSlide orOverhead
?YES, then keep it
NO, then toss it
Who’s your Daddy?
Does the data address the question?
Results Section
•Longest section = .7 T•Logical flow•Avoid vague statements•Draw conclusions (different from THM)
Conclusion:
Mutations in abc1 cause abnormal eye development
THM:
Eye development is regulated by ubiquitylation
Know Your Data
•Practice without it
You must know:
•What the data are•What the data mean•Where the data lead
Step 3 The Conclusion
•Recap the question
•Recap the most important conclusion(s)
•State the THM
For Clarity -The Three Tellums
1. Tellum what you’re gonna tellum
2. Then tellum
3. Then tellum what you toldum
Slides - audiovisual aids
(The important word is “aid”. Remember….this is a TALK)
Tor signaling
•Tor is a highly conserved PI3 (lipid) kinase that is a protein kinase in vitro, and presumably in vivo.•So-called because it is the “target of rapamycin”•Rapamycin is a drug that was identified from the soil of the island of “Rapa Nui” (also known as Easter Island) in a screen for anti-fungal compounds•Yeast mutants resistant to rapamycin identified three genes TOR1 TOR2 and FRP1•FRP1 encodes a homolog of FK-506 binding protein•It is rapamycin bound to FK-506 binding protein that binds to and inhibits the Tor1 and Tor2 protein kinases
Here’s an example of a really bad slide.(Nobody came to see if you can read)
YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
Don’t show figures from papers with no context
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Keep it simple.This slide explains the important experiment
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Colors are not necessarily good
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Background colors are OK but be careful
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Avoid Powerpoint backgrounds orThe curtain goes down on your career
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Sometimes nice to break things up
YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
VERY BUSY
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Isolate the important information (still too busy)and lots of white space
Pull down Mad3-PrA
(Blot Mad2)
WCE
SUP
Pulldown Cdc20-13Myc
Mad3
PrA
Mad2 Mad2
Cdc20
Myc
(Blot Mad2)
Sequential Immunoprecipitation
Simplify with title, graphic, essential words
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Isolate the important data
Mad3
PrA
Mad2 Mad2
Cdc20
Myc
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Even better
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
Avoid Powerpoint Nonsense
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
or find a new career
Don’t get carried away!
“Every product of genius is a product of enthusiasm”
Benjamin Disreali
The Delivery
Be yourself and for Pete’s sake RELAX!
And finally…………..
Dress for Success
Men – Business Casual
Women – Professional not Provocative
The Ten Minute Rule
No matter what the talk/seminar is:
T/2 slides
Be professional
My Take Home Message: