sanko university innovation in medicine summit-3, 11-13 may … · 2017. 6. 13. · sanko...

SANKO University Innovation in Medicine Summit-3, 11-13 May 2017, Gaziantep, Turkey.

1 The Congress Is Supported By The Scientific And Technological Research Council Of Turkey (TUBITAK)



Honorary President Prof. Dr. Güner DAĞLI

SANKO University Rector Surrogate

Congress President Prof. Dr. Eyüp İlker SAYGILI

Congress Vice President Assoc. Dr. Zafer ÇETİN

Congress Secretary Assist. Prof. Dr. Ayşegül ÇÖRT

Assist.Prof. Dr. Necla BENLİER

Execution Commite

Prof. Dr. E. İlker SAYGILI Assoc. Prof. Dr. Zafer ÇETİN

Assist. Prof. Dr. Necla BENLİER Assist. Prof. Dr. Ayşegül ÇÖRT

Msc. Ayşenur SARI Instructor Deniz MIHÇIOĞLU

SANKO University Activity Execution Commite

Prof. Dr. Can Polat EYİGÜN (Member) Dr. Yusuf Ziya YILDIRIM (Member)

Prof. Dr. Türkan PASİNLİOĞLU (Member) Prof. Dr. Salih Murat AKKIN (Member)

Prof. Dr. Mehmet BAŞTEMİR (Member) Prof. Dr. Ayşen BAYRAM (Member)

Ebru YAPAN (Member) Lab. Coordinator Nilay UÇAR (Member)

Funda Esin FAKILI (Member) Tarkan YILDIRIM (Member)

Eda ÖĞÜT (Member)



International Sciencetific Commite

Prof. Dr. Aldo TOMASI (Universita Degli Modena Departmant of Diagnosis Medicine)

Prof. Dr. Biaoyang LIN (University of Washington, Seattle, Washington, USA and Systems Biology Division, Zhejiang-California

International Nanosystems Institute (ZCNI) of Zhejiang University, Hangzhou, China)

Prof. Dr.Carla FERRERI (National Research Council of Italy (CNR) The Institute of Organic Synthesis and Photoreactivity (ISOF))

Prof. Dr. Collet DANDARA (University of Cape Town, Cape Town, South Africa)

Prof. Dr. Chryssostomos CHATGILIALOGLU (National Centre of Scientific Research "Demokritos", Athens, Greece Institute of Nanoscience and

Nanotechnology)

Prof. Dr. Collet DANDARA (University of Cape Town, Cape Town, South Africa)

Prof. Dr. Mehmet Vural ÖZDEMİR (Editör - OMICS; A Journal of Integrative Bıology)

Prof. Dr. Wei WANG (Edith Cowan University, Perth, Australia)

Assoc. Prof. Dr. Alexandros GEORGAKILAS (National Technical University of Athens DNA Damage and Repair Laboratory)

Assoc. Prof. Dr. Sanjeeva SRIVASTAVA (Proteomics Laboratory, Indian Institute of Technology Bombay (IITB), Mumbai, India)

Dr. Ahmed RATTANI (Harvard Medical School Mount Auburn Hospital)

Dr. Alexander BORDA-RODRIGUEZ (Open University, United Kingdom)

Dr. Farah HUZAIR



(School of Social and Political Science, The University of Edinburg, United Kingdom)

Dr. Rita PODZUNA (SCHÖDINGER SCHÖDINGER GERMANY)

Dr. Samir SOFTİC (Harvard Medical School Boston Children’s Hospital)

Dr. Zacharenia NIKITAKI (National Technical University of Athens DNA Damage and Repair Laboratory)

National Sciencetific Commite

Prof. Dr. Ayhan DEVİREN (İstanbul University Medicine School, Scientific Biology Master of Science)

Prof. Dr. Burhan ATEŞ (İnönü University, Faculty of Science And Literatury Chemistry Department )

Prof. Dr. Belgin SÜSLEYİCİ DUMAN (Marmara University, Faculty of Science And Literatury Biology Department)

Prof. Dr. Cengizhan ÖZTÜRK (Bosphorus University Biomedical Engineer Institute)

Prof. Dr. Dildar KONUKOĞLU (İstanbul University Cerrahpaşa Medicine School Scientific Biochemistry Master of Science)

Prof. Dr. Engin ULUKAYA (İstinye University Medicine School Scientific Biochemistry Master of Science)

Prof. Dr. Nezih HEKİM (Biruni University Natural Science And Engineering Faculty Molecular Biology And Genetics Department)

Prof. Dr. Haydar BAĞIŞ (Adıyaman University Medicine School Scientific Genetics Master of Science)

Prof. Dr. İlhan ONARAN (Istanbul University Cerrahpaşa Medicine School Scientific Biology Master of Science )

Prof. Dr. İsmet YILMAZ



(İnönü University Faculty of Science Literature Chemisrty Department)

Prof. Dr. Kadir BATCIOĞLU (İnönü University Pharmacy Faculty Basic Pharmacy Master of science)

Prof. Dr. Melek ÖZTÜRK SEZGİN (İstanbul University Cerrahpaşa Medicine School Medical Biology Master of science)

Prof. Dr. Müjgan CENGİZ (İstanbul University Cerrahpaşa Medicine School Medical Biology Master of Science)

Prof. Dr. Turgut ULUTİN (İstanbul University Cerrahpaşa Medicine School Medical Biology Master of Science)

Prof. Dr. Muradiye NACAK (Gaziantep University Medicine School Pharmacy Master of Science)

Prof. Dr. Mehtap ÖZKUR (Gaziantep University Medicine School Pharmacy Master of Science)

Prof. Dr. İlhan ONARAN (İstanbul University Cerrahpaşa Medicine School Medical Biology Master of Science)

Prof. Dr. Sibel GÜRÜN (Uludağ University Medical Pharmacy Master of Science)

Prof. Dr. Sibel BERKER KARAÜZÜM (Akdeniz University Medicine School Medical Biology And Genetics Master of Science)

Prof. Dr. Tomris ÖZBEN (Akdeniz University Medicine School Medical Biochemistry Master of Science)

Assoc. Prof. Dr DURDAĞI (Bahçeşehir University Medicine School Biophysics Master of Science)

Assoc. Prof. Dr. Fatma KAYA DAĞISTANLI (İstanbul University Cerrahpaşa Medicine School Medical Biology Master of Science)

Assoc. Prof. Dr. Selin SAYIN (İskenderun Technical University )



Assoc. Prof. Dr Tuğba TAŞKIN TOK (Gaziantep University Faculty Of Science Literature Chemistry Department)

Assoc. Prof. Dr Meral YÜKSEL (Marmara University Health Sevices Vocatinal School Medical Services And Technics Department)

Assoc. Prof. Dr Muhammed KARAMAN (Kilis 7 Aralık University Faculty Of Science And Literature Chemistry Department)

Assoc. Prof. Dr. Şule BEYHAN ÖZDAŞ (İstanbul Bilim University Medicine School Medical Biology And Genetics)

Assoc. Prof. Dr. Burak ÖNAL (İstinye University Medicene School Pharmacy Master of Science)

Assoc. Prof. Dr. Oktay İ. KAPLAN (Medeniyet University Medicene School Medical Biology Master of Science)

Assist. Prof. Dr Abdulilah ECE (Biruni University Faculty of Pharmacy)

Dr. Furkan BURAK (Harvard Medical School Mount Auburn Hospital)

Dr. Mehmet Şerif CANSEVER (İstanbul University Cerrahpaşa Medicine School Central Laboratory)



Contents

Programme...........................................................................................(9-14)

Oral presentations………………………………………………………(15-65)

Poster Presentations……………………………………………………...(66-128)

Author index………………………………………………………………..(129-131)



May 10, 2017 Wednesday

Moderatör: Assoc. Prof. Tuğba Taşkın TOK

09:30 -

16:30

Course "Computer Aided Drug Design"

Location: SANKO University Digital Laboratory

Manages: Assist. Prof. Abdulilah ECE (Biruni University)

May 11, 2017 Thursday

08:30 -

17:00 Registration

09:30 -

10:00 Opening Speeches

10:00 -

10:15 Coffee Break

PANEL: TURKEY R & D AND INNOVATION ECOSYSTEM

Moderatör: Prof. Canpolat EYIGUN

10:15 -

10:30 Prof. Ali Osman KILIÇ (Health Institutes of Turkey / President of The Turkey Biotechnology Insitute)

10:30 -

10:45 Prof. S. Nezih HEKİM (Biruni University)

10:45 -

11:00 Assoc. Prof. Rana SANYAL (RS Research Company)

11:00 -

11:15 Cengiz AYDIN (Investment Policies & Corporate Communication Director at AIFD)

11:15 -

11:30 Buğraer ASLAN (Group Director, Oncology at HASBIOTECH)

11:30 -

12:00 Discussion

12:00 -

12:10 Photo Shooting

12:10 -

13:00 Lunch

SESSION 1

SESSION CHAIRMAN: Prof. Güner DAĞLI, Prof. Ayşen BAYRAM

http://www.tiptainovasyon.org/Inovasyon/kurs/index.html





13:00 -

13:30 Prof. Mustafa CAMGÖZ

“Innovation in Cancer: Role and Clinical potential of Ion Channel in Metastasi”

13:30 -

14:00 Prof. Erem BİLENSOY "Nano-drugs: Pre-Clinical and Clinical Evaluation, Market Status and R & D"

14:00 -

14:15 Coffee Break

SESSION 2

SESSION CHAIRMAN: Prof. Lütfi ÇAKAR, Assoc Prof. Selman ÜNVERDİ

14:15 -

14:45 Assoc. Prof. Alexandros GEORGAKILAS "Drug Design with Nanoparticles"

14:45 -

15:15 Dr. Robert JABULOWSKY

“Noval Mrna Cancer Immunothreapies”

SESSION 3

Salon 1

SESSION CHAIRMAN: Prof. Eyüphan YAKINCI,

Assist.Prof. Burak ÖNAL

Salon 2

SESSION CHAIRMAN: Prof. Vildan SÜMBÜLOĞLU,

Assist.Prof. Necla BENLİER

15:15 -

15:25

Marina ORUÇ ‘’The Treatment of Chronic Ear Pruritus and

Interdisciplinary Approaches to This Issue Clinical

Medicine Researches’’

15:15 -

15:25 Assist.Prof. Yücel BAŞPINAR ’Ultrasound Enhanced Gene Delivery With Nanobubbles’’

15:25 -

15:35 Arzu Dinçer ‘‘Legal Procedures from Molecule to Product’’

15:25 -

15:35

Dr.Öznur GÜNGÖR ‘’Simultaneous Determination of Melatonin and Dopamine

Based on Graphene Composite

Modified Electrodes’’

15:35 -

15:45

Dr. Muhammet KARAMAN "A New Approach For Cancer Treatment: Targeting

Nucleotide Excision Repair Mechanism"

15:35 -

15:45

Assist. Prof. Süray PEHLİVANOĞLU "Novel Cu(II) Complexes of 2-Hydroxy-5

Methoxyacetophenone

Thiosemicarbazone and Its N(4)- Substituted Derivatives

and Their Spectral, Voltammetric,

Dft, and Biological Studies

15:45 -

15:55 Assoc. Prof. Hülya ÇİÇEK "The Roles of Antiangiogenic Factors in Preeclampsia"

15:45 -

15:55

Res. Asst. Hatice Gamze SOĞUKÖMEROĞULLARI "Novel Synthesis of Pd(II) Metal Complexes

of SNS Pincer Type Thioether Ligands: High Efficient

Catalyst Precursors for Synthesis

Vitamin K3"

15:55 -

16:05

Res.Asst. Bahadır BATAR "WWOX: Role In Dna Double-Strand Break Repair

Pathway Choice"

15:55 -

16:05

Res.Asst. Nazlı ERDOĞAR "Novel Folate-Conjugated Amphiphilic Cyclodextrın

Nanoparticles For Paclitaxel

Delivery In Recurrent Metastatic Breast Cancer"



16:05 -

16:15 Break

Salon 1

SESSION CHAIRMAN: Assist Prof. Elif PALA, Assist.Prof. Demet

ARI

Salon 2

SESSION CHAIRMAN: Assoc.Prof. Zafer ÇETİN,

Assist.Prof. Ayşegül CÖRT

16:15 -

16:25

Assist.Prof. Tahir ÇAKIR "Radyoprotective Profile of Uritica Dioica L. Seed Extract

On Oxidative DNA Damage in Liver Tissue and Whole

Blood of Radiation Induced Rats"

16:15 -

16:25

Assoc.Prof. Cumhur KIRILMIŞ "Synthesıs Of Dısulfıde Brıdge

Bıs-Dıthıazoleamıne Schıff Bases"

16:25 -

16:35

Assist.Prof. Serkan GÜRGÜL "Anti-IgE Therapy Prevents Chronic Allergic Asthma-

Related Deterioration Of Bone Quality In Asthmatic Mice:

A Biomechanical & Icp-Ms Study"

16:25 -

16:35

Assist.Prof. İncilay GÖKBULUT "Investigation of In Vivo Pharmacokinetic of

Microcapsuled Rosemary Essential Oil"

16:35 -

16:45

Assist.Prof. Erdal UYSAL "Comparı·son of Effects of The Tacrolı·mus and

Cyclosporı·ne A On The Colon Anastomosı·s Recovery of

Rats"

16:35 -

16:45

Assist.Prof. Mecit ÖZDEMİR "A selective fluorescent ‘turn-on’ sensor for recognition of

Zn2+ in aqueous media"

16:45 -

16:55

Assist.Prof. Günnur KOÇER "The Contrı·butı·on of Swı·mmı·ng Exercı·se To Carbon

Monoxı·de Relaxatı·on Responses in Aged Rats"

16:45 -

16:55

Res.Asst. Nurcan KARAMAN "Synthesis, Biological and Theoretical

Evaluations of Novel Piperidine- Hydrazone Derivatives"

16:55 -

17:05

Assoc.Prof. Mehmet DEMİR "Age-Related Changes In Type I Collagen and MMP-2

Expression in Mice"

16:55 -

17:05

Dr. Merve Gökşin KARAASLAN "Preparing Cardiac-Implantable Electrophysiological

Polyurethane Films Against the Post-Implantation

Infection Problem"

17:05 -

17:15

Dr. Mustafa ÇİÇEK "An Experimental Study: Effect of Acupuncture in Wound

Healing"

17:05 -

17:15

PhD. Badr Q. ISMAEL "Antioxidant Activity and Antimicrobial Activity

of Crude Extract From Some Medicinal Plant on

Four Pathogenic Bacteria"

17:15 -

17:25

Fatma Kübra TOMBULTÜRK "Effects of Sericata on Rat Cutaneous Wounds

Healing:Regulation of Collagen I and Collagen III by Nf-Kb

(P65) on Diabetic Rats"

17:15 -

17:25

MSc. Akram Mudher Kareem ALJBORI "Effect on Biochemical Parameters and Liver

Histopathology of Malathion Exposure Nigella Sativa in

Rats"

May 12, 2017 Friday

08:30 -

17:30 Registration

SESSION 4

SESSION CHAIRMAN: Prof. Mehmet BAŞTEMİR, Prof. Türkan Pasinlioğlu

09:00 -

09:30 Dr. M. Furkan BURAK "Development of Anti-Ap2 Monoclonal Antibody to Treat Diabetes and Fatty Liver Disease"



09:30 -

10:00 Dr. Samir SOFTIC "Knockdown of Fructose Metabolism as A Therapeutic Target for Treatment of Diabetes and Fatty Liver Disease"

10:00 -

10:30 Dr. Ahmed RATTANI "Shugoshin-2 and Cancer Drug Discovery"

10:30 -

11:15 SATELLITE STMPOSIUM (Hall B)

11:15 -

12:00 SATELLITE STMPOSIUM (Hall B)

12:00 -

13:00 Lunch

SESSION 5

SESSION CHAIRMAN: Prof. Sibel BERKER KARAÜZÜM,

Prof. Şahin SIRMALI

13:00 -

13:30 Prof. Sinan ÇAVUN "Glycl-Glutaminin (Gly-Gln) Molecule and Its Use in The Treatment of Depression"

13:30 -

14:00 Assoc. Prof. Serdar DURDAĞI

“Development of New Generaation AT1 Inhibitors Using Multi-Scale Molecular Modeling Approaches”

14:00 -

14:15 Break

SESSION 6

Salon 1

SESSION CHAIRMAN: Assist.Prof. Nevhiz GÜNDOĞDU,

Assist.Prof. Mümtaz Murat YARDIMCI

Salon 2

SESSION CHAIRMAN: Assist.

Prof.Erdal UYSAL,

Assist.

Prof. Şinasi ÖZKILIÇ

14:15 -

14:25

Prof.Emin Türkay KORGUN "Production, Storage And Preparation to Use in Laboratory

of Placenta Derived Mesenchymal Stem Cells and

Endothelial Cells For Scientific Researches"

14:15 -

14:25

Assoc. Prof. Gülten KAVAK BALCI "İnvestigation of BSA Solutions Depending on

Temperature by NMR"

14:25 -

14:35

Assist. Prof. Ertuğtul ALLAHVERDİ "Antioxidant and Antigenotoxic Effect of Alpha Lipoic Acid

in Obstructive Jaundice"

14:25 -

14:35

Assist. Prof. Hamit Hakan ALP "Beta-Fibrinogen -455 G/A and Angiotensin Converting

Enzyme I/D Gene Polymorphisms İn Patients with Lung

Cancer"



14:35 -

14:45

PhD.Beste TURANLI "Gene Expression Oriented Drug Repositoning Targets For

Prostate Cancer"

14:35 -

14:45

Dr. İlknur ÖZGENÇLİ "Investigation of The Effects of Some

Anticancer Agents on The Activity of

Mitochondrial Thioredoxin Reductase and Cytosolic

Glutathione S-Transferase by Purification, Kinetic and

Molecular Dockins Studies"

14:45 -

14:55

MSc. Cem VARAN "Preparation and Characterization of Antiviral and

Anticancer Drug Printed Film Formulations for the

Treatment of Cervical Cancer"

14:45 -

14:55

PhD. İbrahim Bozgeyik "Screening Of Antioxidant-Anticancer Activity of Mentha

longifolia (L).) Hudson subsp typhoides (Brig) var.

typhoides on HeLa Cancer Cells"

14:55 -

15:05

MSc. Gamze VARAN "Evaluation of Cholesterol-Targeted Amphiphilic

Cyclodextrin Nanoparticles for Cancer Therapy"

14:55 -

15:05

PhD. Khattab AL-KHAFAJI "Structural Insights Into Amygdalin as A Multi-Functional

Anti-Cancer Agent"

15:05 -

15:15

MSc. Burçin Nilay YENER "The Role of Fasudil Treatment on Amyloid Beta Induced

Inflammation Model in Astrocytes"

15:05 -

15:15

MSc. İsmail KAŞOĞLU "Exosome Based Treatments as A New Method

For The Treatment Of Cancer"

15:25 -

15:45 Coffee Break

SESSION 7

Salon 1

SESSION CHAIRMAN: Assist. Prof. Şule Beyhan ÖZDAŞ,

Assist. Prof. Betül KOCAMER ŞİMŞEK

Salon 2

SESSION CHAIRMAN: Assist. Prof.Başar AKSOY,

Assist. Prof. Ünal SARIKABADAYI

15:45 -

15:55

Assist.Prof. Barbaros Şahin KARAGÜN

"Micro-RNA Profile of Childhood Acute Leukemia"

15:45 -

15:55

Res. Asst. Ergün MENDEŞ "Efficiency of Volume Reduction by Application

of Additional Lower Extremity Operations"

15:55 -

16:05

PhD. Emine YAVUZ "Two Wavelength High Intensity Irradiation for the

Effective Crosslinking of DNA to Protein"

15:55 -

16:05 Dr. Aziz YARBİL "Regional Anesthesia in ClavliculaOperations"

16:05 -

16:15

Dr. Özlem DEMİRCİ "Application of 1H NMR-Based Metabolomics for

Toxicological Evaluation"

16:05 -

16:15

Naser GİLANİ "Next Generation Sequencing Reveals

Association of C.2461>A in Col1a1 Gene With

Osteogenesis Imperfecta: A Case"

16:15 -

16:25

Dr. Öznur DOĞAN ULU "Synthesis, Characterization and Antimicrobial Activities of

Ag(I)-N-Heterocyclic Carbene Complexes"

16:15 -

16:25

Rozhgar A. KHAILANY "A Single Nucleotide Polymorphism of Sur1 Gene

Detected in Type 2 Diabetic Patiemts Living in Erbil

Province-Iraq"

16:25 -

16:35

Assist.Prof. Zübeyir HUYUT ''Inhibition Properties of Some Flavonoids on Carbonic

Anhydrase I and II Isoenzymes Purified From Human

Erythrocytes'' "

16:25 -

16:35

PhD. Samir Abbas Ali NOMA "Synthesis of Novel Hydrazone Derivatives and

Investigation of Their Biological Activities"



16:35 -

16:45

Assoc. Prof Gülşen ÖZTÜRK "Afacile synthesis of Amide Based Receptors Under

Microwave Conditions: Investigation of Their Anion

Recognition Properties by Experimental and Computational

Tools"

16:35 -

16:45

PhD. Sevgi BALCIOĞLU "Development of Haemostatic Matrixs Based on

Polyurethane Prepolymer and Biological Applications"

16:45 -

16:55

"Suitability of Magnetic Fe3O4 Nanoparticles in

Hyperthermia Treatment"

16:45 -

16:55 "DNA Damaging Activities of Some PTP1B Inhibitors"

16:55 -

17:05

"Morphological and Chemical Characterization of Spirulina

Platensis to Synthesis of Protein-Based Biopolymers"

16:55 -

17:05

"Synthesis of Calcium Phosphate Biomaterials From Sea

Urchin Skeleton and Comparasion Tith Bone Cement"

May 13, 2017 Saturday

08:30 -

12:00 Registration

SESSION 8

SESSION CHAIRMAN: Assoc Prof. Ayşe Esra MANGUOGLU , Assist . Prof. Murat ULUTAŞ

09:00 -

09:30 Prof. Belgin SÜSLEYİCİ

“Rational Drug Applications to individual Spesific Genetic Sturucture:Pharmocogenetics”

09:30 -

10:00 Prof. Burhan ATEŞ "Preparation of Biocompatible Semisynthetic Tissue Adhesives for Use in Surgical Operations"

10:00 -

10:30 Coffee Break

SESSION CHAIRMAN: Prof. E. İlker SAYGILI, Assoc. Prof. Zafer CETİN, Assist. Prof. Aysegul CORT,

Assist. Prof. Necla BENLİER

10:30 -

11:30 Closing and General Evaluation

11:30 -

17:00 GAZİANTEP City Tour



Oral Presentation

Abstracts for the SANKO University Innovation in Medicine Summit-III

11th-13th May 2017, Gaziantep, Turkey



O.1. The Treatment Of Chronic Ear Pruritus And Interdisciplinary Approaches To This Issue

Clinical Medicine Researches

Marina ORUÇ

Gazikent Mah. 73 Sok. No:11 D:18 Gaziemir-İZMİR

I am Marina ORUÇ; ear, nose and throat specialist (ENT) from Russian Federation.

As a result of more than 13 years research and study I have found a method on “The Treatment Of

Chronic Ottis Externa” and obtained an “innovation” patent which under 20 years protection and

research qualification. I also have made an application to Patent Cooperation Treaty (PCT). My

appointment has been accepted in 151 countries.

The purpose of my invention is to improve the efficiency of the treatment of external auditory conal

disease which causes deformation on ear way and external ear in accompany with heavy pruritus and.

The purpose is also suppressing the glowing of bacterial and fungal atitis externa.

Besides, it is necessary to examine this matter deeply. The standart treatments didn’t take effect. So I

was in need to find a solution to this problem. I postulated this was a disease caused by liver and

gallbladder.

After biochemistry and ultrasound researches and analysis I used hepatoprotektif and gallbladder

medicines and I came to a conclusion on the treatment.

Upon my invention, with the (ENT) dermatologists, venereologist, gastroentologists, research and

development specialists, family doctors, medical examiners, medical ultrasonography specialists and

medicine companies must keep to make researches, expand and deepen their limits. Because itches like

ear itch also might happen on the other sides of the body. We can help millions of patients in our

country and the worldwide with the innovation on the medicine.



O.2.A NEW APPROACH FOR CANCER TREATMENT: TARGETING NUCLEOTIDE

EXCISION REPAIR MECHANISM

Halide Sedef KARAMAN1, Muhammet KARAMAN2

1Atatürk University, Faculty of Sciences, Department of Chemistry, Erzurum, Turkey 2Kilis 7 Aralık University, Faculty of Arts and Sciences, Department of Chemistry, Kilis, Turkey

The Nobel Prize in Chemistry (1/3) was given to Aziz Sancar in 2015 due to DNA repair

mechanism studies. In the studies, Aziz Sancar has illustrated mechanisms repairing UV-induced DNA

damage in E. coli and human. Although UV-induced DNA damage is repaired by photolyase enzyme

and nucleotide excision repair (NER) mechanism in E. coli, nucleotide excision repair is a sole and

unique mechanism used to repair UV-induced damage in human.

The cyclobutane pyrimidine dimer and the 6,4-pyrimidine primidone generated by UV radiation

are excised from the genomic DNA by the activity of the RPA, XPA, XPC, TFIIH, XPD, XPB, XPC

and XPF proteins, which are called main nucleotide excision factors. The incidence of skin cancer in

Xeroderma Pigmentosum patients, in which these factors are deficient, is increased by 5,000-fold

compared to individuals with the normal NER mechanism. The NER mechanism not only repairs the

UV-induced DNA damage, but also leads cisplatin resistance by repairing damage to DNA caused by

cisplatin used in the treatment of testicular, bladder, ovary, ovary, head-neck, cervical, lung and

colorectal cancers. This limits the treatment of other cancer types than testicular cancer. It has been

determined that testicular tumor cells, in which the NER capacity is lower than other cells, are treated

more effectively with cisplatin. NER activity is dependent on the cellular levels of XPA and

XPF/ERCC1 proteins.

In recent years, computer aided drug design is widely used in order to increase efficiency of

cisplatin in cancer treatment. For the targeted inhibition of XPA and in particular XPF/ERCC1 proteins,

in vitro and in vivo activity of many synthesized molecules have been screened. Although researchers’

interest in XPF / ERCC1 inhibition, XPA inhibition is thought to be more effective in increasing

cisplatin activity in the treatment of cancer, as it is controlled by the biological clock via CLOCK-

BMAL1 and has multiple binding sites with other nucleotide excision factors.



O.3. THE ROLES OF ANTIANGIOGENIC FACTORS IN PREECLAMPSIA

Hülya ÇİÇEK1, Hanifi KIZILYER1, Ali Mesut MISIRLIOĞLU2, Hasan ULUSAL1,

Haci Ahmet DEVECİ3, Gökhan NUR3

1University of Gaziantep, Medical Faculty, Department of Medical Biochemistry, Gaziantep/Turkey

2Gaziantep Cengiz Gökçek Obstetrics and Gynecology Hospital, Gaziantep/Turkey

3University of Gaziantep, Islahiye Vocational High School, Gaziantep/Turkey

Objectives: Preeclampsia is a disorder characterized by hypertension and proteinuria after 20th week of

pregnancy. It is he most important cause of maternal and perinatal morbidity and mortality. The

pathophysiology of preeclampsia has not completely understood but it has been proceed with

deterioration in the balance of placental angiogenic and antiangiogenic factors. Therefore, importance of

antiangiogenic factors has recently increased in the etiology of preeclampsia. In our study it has aimed

to determine the level antiangiogenic factors as Soluble Fms-Like Tyrosine Kinase-1 ve sEndoglin and

to define the roles over the course of the disease.

Material and Methods: The research was conducted on patients who have 24-42 weeks of pregnancy

and undergoing treatment at clinics of Gaziantep Cengiz Gökçek Obstetrics and Gynecology Hospital.

41 preeclamptic women and 32 healthy pregnant women matched with maternal age were recruited to

study. Soluble Fms-Like Tyrosine Kinase-1and sEndoglin levels were measured with ELISA method

and haemogram, urinalysis, biochemical parameters such as glucose, BUN, creatinine, ALT, AST,

potassium levels were assessed in the serum samples and evaluated together.

Results: Comparisons between patient and control groups showed that Soluble Fms-Like Tyrosine

Kinase-1 and sEndoglin levels were significantly higher in the patient group than the control group (p

<0,05).

Conclusion: According to our findings, Soluble Fms-Like Tyrosine Kinase-1 and sEndoglin levels of

preeclamptic patients were found to be higher than healthy pregnancies. Thus, we believe that these tests

can be used effectively in the diagnosis and follow-up of preeclampsia, based on previous studies and

our own data.

Keywords: Preeclampsia, Antiangiogenic factor, Soluble Fms-Like Tyrosine Kinase-1, s Endoglin



O.4.WWOX: ROLE IN DNA DOUBLE-STRAND BREAK REPAIR PATHWAY CHOICE

Bahadir BATAR1*, Morgan S. SCHROCK1, Jaeho LEE2, Teresa DRUCK1, Brent FERGUSON2,

Ju HWAN CHO3, Kenneth AKAKPO1, Hoda HAGRASS1, Nyla A. HEEREMA4, Fen XIA3,

Jeffrey D. PARVIN5, C. Marcelo ALDAZ2, Kay HUEBNER1

1The Ohio State University, Cancer Biology and Genetics, Columbus, USA. 2The University of Texas, Epigenetics and Molecular Carcinogenesis, Smithville, USA. 3The Ohio State University, Radiation Oncology, Columbus, USA. 4The Ohio State University, Pathology, Columbus, USA. 5The Ohio State University, Biomedical Informatics, Columbus, USA.

*Current address: Department of Medical Biology, Cerrahpasa Medical School, Istanbul University,

Istanbul, Turkey

Background: Expression of Wwox protein is frequently lost or reduced in many human cancers.

Human and mouse cells deficient for Wwox exhibit significantly enhanced survival of ionizing

radiation (IR) and Mitomycin C (MMC) treatment, agents that induce DNA double-strand breaks

(DSBs). Our aim was to identify the DSB repair pathway used by Wwox-deficient cells.

Material and methods: To define if Wwox expression may affect repair of induced DSBs, we

investigated the effects of IR and chemotherapeutic agents on Wwox-deficient vs sufficient cells. Mouse

embryonic fibroblasts (MEFs) were exposed to various IR doses and agent concentrations and plated for

clonogenicity to analyze cell survival and proliferation. To elucidate the mechanism that underlies

Wwox-deficiency associated resistance, we performed pathway specific recombinant plasmid reporter

assays.

Results: We observed a significant difference (P<0.01) in survival at doses 7.7 Gy and higher, with

knockout (KO) lines KO3 and KO5 surviving 10-fold better than wild type (wt) cell lines WT4 and

WT7. Survival curves for shWWOXA and shWWOXB transformed clones (breast epithelial cell lines,

MCF10A) also demonstrated increased survival at 7.7 Gy and above (P<0.05) vs the Wwox-sufficient

shScrambled transfected cells. Furthermore, KO MEFs exhibited enhanced survival to 4 hr MMC

exposure at concentrations 1 μM and higher (P<0.01). Also, we found that radiation resistance in

Wwox-deficient cells is caused by enhanced homologous-directed repair (HDR).

Conclusions: Wwox deficiency promotes enhanced HDR efficiency. Selectively inhibiting the HDR

pathway in Wwox-deficient cells may abolish their radio-resistance and re-sensitize them to radiation,

suggesting the use of HDR inhibitors in conjunction with radiation for treatment of Wwox-deficient

tumors.

Key Words: Double-strand break, Homology-directed repair, Radio-resistance, Wwox



O.5.ULTRASOUND ENHANCED GENE DELIVERY WITH NANOBUBBLES

Yücel BAŞPINAR

Ege University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, İzmir

Objectives: Ultrasound (US) responsive drug delivery systems like nanobubbles (NBs) have become an

increasing focus of research in the last years. Here, it was aimed to outline US enhanced gene delivery

with NBs.

Methods: NBs, with a size <1 μm, are composed of core and shell. For the core hydrophobic gases like

perfluorocarbons (PFCs) and for the shell polymers can be used.

Results: NBs with diethylaminoethyl-dextran shell and perfluoropentane core were prepared as DNA

transfection agents. They promoted the entry of DNA into COS-7 cells, protected DNA from nucleases

and transfect it across the cell membrane. A moderate transfection for COS-7 cells was obtained with

chitosan-NBs following 30 seconds of US treatment. NBs containing sirtuin-2 small interfering RNA

(siRNA) were developed for glioma tumor therapy and resulted in an accumulation of siRNA-NBs in

tumor tissues following US exposure, achieved by enhanced permeability and retention (EPR) effect,

indicating that US-sensitive siRNA-NBs may possess great potential in cancer treatment. Treatment

with NBs carrying androgen receptor siRNA combined with US significantly inhibited cell growth and

resulted in suppression of AR messenger RNA and distributed more widely in prostate tumor.

Conclusions: Exposure to US lead to liquid to gas phase transition of PFCs, having low boiling points.

The transport of genetic material into the cell is enabled by EPR, caused by shear stress, leading to

further drive into cytoplasm. Combining NBs with US resulted in improved transfection efficiency of

genetic material due to cavitations in capillary vessels and in tumor tissue.

Key words: nanobubble, ultrasound, gene delivery, siRNA, cancer



O.6.SIMULTANEOUS DETERMINATION OF MELATONIN AND DOPAMINE BASED ON

GRAPHENE OXIDE/POLYIMIDE COMPOSITE MODIFIED ELECTRODES

Öznur Güngör1, Aziz Paşahan1 , Büşra Aksoy1 ,Süleyman Köytepe1, Turgay Seçkin1

1İnönü University, Faculty of Arts and Science, Chemistry Department, 44280, Malatya, Turkey

[email protected]

Melatonin as hormone primarily secreted by the pineal gland, has wide functions in vertebrate

organisms, with effects almost on all tissues and organs[1]. Melatonin modulates neurogenesis, synaptic

functions, neuronal cytoskeleton and gene expression[2]. Additionally, melatonin is a powerful

endogenous antioxidant involved in the prevention of the oxidative stress both through its direct free

radical scavenging effect and by increasing antioxidant activity[3]. Dopamine is one of the most

significant catecholamines in the brain and belongs to the family of excitatory chemical

neurotransmitters. Uncommon levels of dopamine may result in drastic neurological disorders such as

schizophrenia, Alzheimer's, Parkinson’s disease and addiction[4].

The prepared graphene oxide/polyimide films as selective membrane for amperometric

dedection of melatonin and dopamine were characterized for their structure, morphology, and thermal

behavior by Fourier transform infrared spectroscopy, scanning electron micrograph, X-ray diffraction,

and thermal analysis techniques. The distribution of graphene particles inside the polyimide composite

films was investigated by scanning electron microscopy. Graphene particles were well dispersed in the

polyimide matrix. The graphene oxide-containing polyimides were formed by casting the film on the

electrode surface. Finaly, polyimide modified electrodes showed lineer, selective and sensitive result for

melatonine and dopamine.

References

[1] G. Tosini, S. Owino, J.L. Guillaume, R. Jockers. Bioessays, 36 (2014), pp.778–787.

[2] G. O'Neal-Moffitt, J. Pilli, S.S. Kumar, J. Olcese. Neuroscience, 277 (2014), pp. 506–521

[3] J.J. García, L. López-Pingarrón, P. Almeida-Souza. J. Pineal Res., 56 (2014), pp. 225–237

[4] R.M. Wightman, L.J. May, A.C. Michael. Anal. Chem. 60 (1988) 769A–779A.

mailto:[email protected]



O.7.NOVEL CU(II) COMPLEXES OF 2-HYDROXY-5 METHOXYACETOPHENONE

THIOSEMICARBAZONE AND ITS N(4)- SUBSTITUTED DERIVATIVES AND THEIR

SPECTRAL, VOLTAMMETRIC, DFT, AND BIOLOGICAL STUDIES.

Suray PEHLIVANOGLU1, Gokce ERDOGAN2, Ercan TURKKAN3, Emine Guler AKGEMCI4

1 Necmettin Erbakan University, Faculty of Science, Department of Molecular Biology and Genetics,

Konya. 2 Akdeniz University, Faculty of Medicine, Department of Medical Biology and Genetics, Antalya. 3 Necmettin Erbakan University, Ahmet Kelesoglu Faculty of Education, Department of Physics

Education, Konya. 4 Necmettin Erbakan University, Ahmet Kelesoglu Faculty of Education, Department of Chemisrty

Education, Konya.

Thiosemicarbazones and their metal complexes are compounds that possess antimicrobial and

anticancer properties. Newly synthesized copper complexes of 2-hydroxy-5-methoxyacetophenone

thiosemicarbazone and its N(4)-substituted derivatives were characterized by theoretical DFT studies

and experimental UV-Vis, FT-IR, EPR spectral analysis, cyclic voltammetry, magnetic susceptibility

and conductivity measurements. The DFT calculation results have been used to predict the experimental

results. The geometric parameter G within the range of 7.61-7.86 for all complexes confirms the

mononuclear nature of the complexes. The EPR, UV-Vis, DFT studies and obtained bonding parameters

show that all the complexes have square planar geometry and their M-L bonds have strong ionic and

some in-plane s-bond character. In addition, the experimental and DFT studies showed that HOMO and

LUMO energy levels of the complexes may present good electron transporting properties. Also, the

investigated Cu(II) complexes were tested for biological activity, proving both in vitro antibacterial and

anticancer activity. The complexes exhibited antibacterial activity against Gram positive bacteria S.

aureus while exhibiting no activities against gram negative bacteria E. coli and S. gallinarum. The f

parameters obtained experimentally by EPR support the antimicrobial activity properties results of the

complexes. The evaluations of potential anticancer activity of these complexes were carried out against

highly metastatic MDA-MB-231 breast adenocarcinoma cell line by MTT assay. Our results suggest

that all tested copper complexes have high cytotoxic effects with the range of 1.76-3.53 μM IC50 values

in vitro. These copper complexes could be considered as potential anticancer agents to counteract drug

resistance of metastatic cancer cells.



O.8.Novel synthesis of Pd(II) metal complexes of SNS pincer type thioether ligands: High efficient

catalyst precursors for synthesis vitamin K3

Hatice Gamze SOGUKOMEROGULLARI1, Serhan URUŞ2, Mehmet SÖNMEZ1

E-mail: [email protected]

1Gaziantep University, Arts and Sciences Faculty, Department of Chemistry, 27310 Gaziantep, Turkey

2Kahramanmaraş Sütçü İmam University, Faculty of Science and Letters, Department of Chemistry,

46100 Kahramanmaraş, Turkey

Pincer type metal complexes have received considerable attention because of their various structural

properties, biological and catalytic applications [1,2]. Vitamin K3 a synthetic derivative of vitamin K

family is responsible for blood coagulation and important for controlly binding of calcium in bones and

other tissues. Besides, it is used for new-borns for the deficiency of vitamin K. [3,4] Moreover, Vitamin

K3 is a compound of hope for cancer treatment because it has antitumor activity.

New four pincer type metal complexes were synthesized from reaction of palladium acetate and SNS-1,

SNS-2, SNS-3 and SNS-4 ligands, respectively. Pincer type metal complexes were characterized by

elemental analysis, UV-Vis, Mass, FT-IR and TGA/DTA spectral analysis. These complexes were used

as catalysts for synthesis vitamin K3. SNS-2 Pd(II) complex showed the best catalytic activity.(% 87.84)

CH3 CH3

O

O

12 h, CH3CN, CH3COOH, H2SO4

SNS-1 R1=CH3, R2=CH3

SNS-2 R1=H, R2=CH3

SNS-3 R1=H, R2=OCH3

SNS-4 R1=H, R2=Cl

N

SSR1

R2

R1

R2

PdAcO OAc

Figure1. Synthesis of K3 vitamin

*This study is supported by a grant (Project number: FEF.13.06) from Presidency of Scientific Research

Projects of Gaziantep University.

Keywords: Pincer type metal complexes, characterization, K vitamin

References

1. H. F. Abd El-Halim, G. G. Mohamed; Journal of Molecular Structure. 2016, 1104, 91-95.

2. A.M. Masdeu-Bultó, M. Diéguez, E. Martin, M. Gómez; Coordination Chemistry Reviews. 2003, 242, 159-201.

3. J. Kowalski, J. Płoszynska, A. Sobkowiak; Catalysis Communications. 2003, 4, 603–608.

4. M. Florea, R.S. Marin, F.M. Palaşanu, F. Neatu, V.I. Pârvulescu; Catalysis Today. 2014, 254, 29–35.



O.9.NOVEL FOLATE-CONJUGATED AMPHIPHILIC CYCLODEXTRIN NANOPARTICLES

FOR PACLITAXEL DELIVERY IN RECURRENT METASTATIC BREAST CANCER

Nazlı ERDOĞAR1, Güneş ESENDAĞLI2, Thorbjorn T. NIELSEN3, Güldal ESENDAĞLI-

YILMAZ4, Mustafa F. SARGON5, Levent ÖNER1, Erem BILENSOY1*

1Hacettepe University Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara,

Turkey

2Hacettepe University Cancer Institute, Department of Basic Oncology, Ankara, Turkey

3University of Aalborg, Faculty of Engineering and Science, Department of Biotechnology, Chemistry

and Environmental Engineering, Aalborg, Denmark

4Gazi University, Faculty of Medicine, Department of Pathology, Ankara, Turkey

5Hacettepe University Faculty of Medicine, Department of Anatomy, Ankara, Turkey

The objective of this study was to design and evaluate paclitaxel loaded actively targeted nanoparticles,

using two new folate-conjugated amphiphilic cyclodextrin derivatives (FCD-1 and FCD-2) synthetized

by our group.

Blank and paclitaxel-loaded FCD nanoparticles were prepared by nanoprecipitation method. The

optimization of formulation variables were carried out using 32 factorial design. Encapsulation

efficiency was evaluated by an indirect method with HPLC. In vitro release studies were obtained by

dialysis membrane method. Cytotoxicity and anticancer efficacy of nanoparticles were determined with

MTT assay. In vivo antitumor efficacy of cyclodextrin nanoparticles was evaluated in terms of survival

rate, tumor size, weight change, metastasis, histopathological appearance using tumor-bearing mice.

Nanoparticles can be formulated in spherical shape and suitable particle size around 100 nm with

improved encapsulation efficiency. Zeta potentials were neutral and -20 mV for FCD-1 and FCD-2

nanoparticles, respectively. Drug release studies showed initial burst release followed by a sustained

release in 12h. Blank cyclodextrin nanoparticles were nontoxic against L929 cells. PCX-loaded

nanoparticles were efficiently engulfed by T-47D and ZR-75-1 breast cancer cells. Additionally, these

cells became more sensitive to cytotoxic and/or cytostatic effects of PCX delivered by FCD-1 and FCD-

2. During cellular uptake studies, nanoparticles internalized into cells were shown. In breast cancer

model, anticancer activity of nanoparticles were compatible with that of paclitaxel in Cremophor®

however repeated administrations of FCD-1 nanoparticles were better tolerated by the animals. These

nanoparticles were able to localize in tumor site. Both paclitaxel-loaded FCD-1 and FCD-2 significantly

reduced tumor burden while FCD-1 significantly improved the survival.

In conclusion, these novel folate-conjugated cyclodextrin nanoparticles can therefore be considered as

promising alternative systems for safe and effective delivery of paclitaxel with folate-dependent

mechanism.



O.10.RADYOPROTECTİVE PROFİLE OF URİTİCA DİOİCA L. SEED EXTRACT ON

OXİDATİVE DNA DAMAGE İN LİVER TİSSUE AND WHOLE BLOOD OF RADİATİON

INDUCED RATS

Tahir ÇAKIR1, Kenan YILDIZHAN1, Zübeyir HUYUT2, Ahmet UYAR3

1 Yuzuncu Yil University, Faculty of Medicine, Department of Biophysics, Van, Turkey 2 Yuzuncu Yil University, Faculty of Medicine, Department of Biochemistry, Van, Turkey

3 Yuzuncu Yil University, Faculty of Veterinary, Deparment of Pathology, Van, Turkey

Abstract

Aim: Ionizing radiation is commonly used to treatment options in the majority of cancer patients, but

radiotherapy applications leads to oxidative DNA damage in non-targeted tissues. In this study, we

investigated the radioprotective effects of Uritica dioica L. seed extract (UDSE) against oxidative DNA

damage in whole blood and liver tissues of irradiated rats.

Methods: 32 rats (eight weeks) divided into 4 group (n:8). Control group (C); was fed with pellet for 10

days. Radiation group (IR) was fed with pellets for 10 days after exposing 5Gy radiations as a single

fraction. Radiation+UDSE (IR+UDSE) group was exposed 5Gy radiations as a single fraction and was

fed with UDSE for 10 days. UDSE group (UDSE) was fed with only UDSE for 10 days. The rats were

sacrificed under anesthesia (50mg/kg ketamine). 8-hydroxy-2-deoxyguanosine (8-OHdG) and dG levels

were measured by high pressure liquid chromatography(HPLC) method in liver tissue and whole blood.

Histopathological changes in the liver tissues were determined by immunohistochemistry.

Results: 8-OHdG/106dG levels of liver tissues and whole blood were increased and degenarations were

observed in liver tissues in IR group (p˂0.05). However, 8-OHdG/106dG levels of liver tissue and whole

blood, and degenarative changes of liver tissues were decreased in the IR+UDSE group compared to the

IR group (p˂0.05). 8-OHdG/106dG levels also were lower in whole bloods of UDSE group, and we did

not observe any degeneration in liver tissues of this group.

Conclusions: It was observed that radiation increased oxidative DNA damage and caused to

degenerative changes in liver. But, after exposure to radiation, the administration of UDSE that has

antioxidant properties may reduce oxidative DNA damage and degenerative changes revealed by the

effects of radiotherapy.

Key Words: Antioxidant, Urtica dioica, ionizing radiation, oxidative DNA damage, oxidative stress.



O.11.ANTI-IgE THERAPY PREVENTS CHRONIC ALLERGIC ASTHMA-RELATED

DETERIORATION OF BONE QUALITY IN ASTHMATIC MICE: A BIOMECHANICAL &

ICP-MS STUDY

Serkan GURGUL1*, Ozlem KESKIN2, Can DEMIREL3, Mehmet Yasar OZKARS2, Yahya

NURAL4

1Department of Biophysics, Faculty of Medicine, Gaziosmanpasa University, TR-60100 Tokat, Turkey 2Department of Pediatric Allergy and Immunology, Faculty of Medicine, Gaziantep University, TR-

27310 Gaziantep, Turkey 3Department of Biophysics, Faculty of Medicine, Gaziantep University, TR- 27310 Gaziantep, Turkey 4Department of Analytical Chemistry, Faculty of Pharmacy, Mersin University, TR-33169 Mersin,

Turkey

*E-mail: [email protected]

Aim: Recent studies suggest an association between allergic diseases, including asthma, and bone loss.

However, it is not yet clearly known whether chronic allergic asthma (CAA) cause bone deterioration

and/or anti-IgE therapy used in asthma treatment prevent bone quality. Thus, the aim of the study was to

investigate whether: i) CAA cause meaningful changes in bone quality and ii) anti-IgE therapy have

protective effects on asthma-related bone loss and bone deterioration if any.

Methods: We used a chronic inhalational exposure model of asthma in ovalbumin-sensitized BALB/c

male mice (8-10 weeks-old) to generate CAA. Thirty-two mice were assigned randomly into four

groups (eight mice per group): control (intact group), CAA (treated with saline), CAA+100 µg of anti-

IgE, and CAA+200 µg of anti-IgE groups. After immunization, saline or anti-IgE administrations were

performed intraperitoneally in five sessions at 15-days interval. Three-point bending test was chosen for

the mechanical analysis. Bone mineral composition (Ca+2, P, Mg, Se, and Ca/P-ratio) was evaluated

using ICP-MS.

Results & Discussion: Our findings showed that CAA can cause bone deterioration and the

deteriorative effects of CAA on bone quality and strength can be prevented by administering 200 µg of

anti-IgE. In addition, we also found that administration of 200 µg of anti-IgE has protective effects on

mineral loss and bone integrity against CAA-related impairment. However, 100 µg of anti-IgE failed to

show these positive effects. It can be suggested that the bone strength and quality is reduced in CAA and

CAA also cause a mineral imbalance in bone. Anti-IgE therapy may dose-dependently inhibit these

impairments via a possible mechanism of preventing both collagen degradation and bone loss.

Keywords: Anti-IgE, Asthma, Biomechanics, Bone loss, Collagen, ICP-MS, Mice.



O.12.COMPARİSON OF EFFECTS OF THE TACROLİMUS AND CYCLOSPORİNE A ON

THE COLON ANASTOMOSİS RECOVERY OF RATS

Erdal UYSAL1, Mehmet DOKUR2

1Sanko University Department of General Surgery, Gaziantep 2Necip Fazıl City Hospital,

Department of Emergency, Kahramanmaras

Purpose: This study aims to examine and compare the effects of immunosuppressant CsA and TAC on

colon anastomosis recovery.

Method: Forty rats were randomly divided into four groups. The four groups were determined as

follows: Control group; Sham group, given %0.09 NaCl; Tacrolimus group (TAC), given 0,5 mg/kg/day

Tacrolimus; and Cyclosporine A group (CsA), given 5 mg/kg/day CsA. A six cm mid-abdomen incision

was performed on the rats. An incision of full layers on the right colon was performed. Then

anastomosis was undertaken. Laparotomy was performed on the seventh day post-operation. The colon

bursting pressures were evaluated, histopathological examinations were undertaken, and E-cadherin

expression and tissue hydroxyproline levels were evaluated.

Result: Statistically significant differences were observed among bursting pressures of the groups

(p<0.001). The value was significantly low in TAC and CsA groups when compared to control and

sham groups (p <0.05). The tissue Hydroxyproline levels were significantly low in TAC group

compared to control group (p=0.03). Fibroblast density and neovascularization were significantly

greater in the control group compared to the TAC group (p<0.05). Levels of collagen had decreased

significantly in the TAC group compared to other groups (p<0.05).

Conclusion: Our study showed that TAC may have a negative effect of colon anastomosis recovery.

The lowest anastomosis bursting pressure was detected in TAC Group. Also Collagen, hydroxyproline,

fibroblast, neovascularization and E-Cadherin levels were comparatively lower in the TAC group. CsA

did not cause any significant changes to tissue hydroxyproline, collagen, fibroblast, and E-Cadherin

levels.

Keywords: Tacrolimus, Cyclosporine A, Rat, Colon, Anastomosis



O.13.THE CONTRIBUTION OF SWIMMING EXERCISE TO CARBON MONOXIDE

RELAXATION RESPONSES IN AGED RATS

Günnur KOÇER1, Seher ÜLKER2, Yusuf OLGAR3, Nihal ÖZTÜRK ERBOĞA3, Semir

ÖZDEMİR3

1Near East University, Faculty of Medicine, Department of Physiology, Nicosia

2Akdeniz University, Faculty of Medicine, Department of Physiology, Antalya

3Akdeniz University, Faculty of Medicine, Department of Biophysics, Antalya

Introduction: Endothelium-dependent dilation reduces during aging. Carbon monoxide (CO) is as an

important endogenous vasodilator and may show a compensatory effect in nitric oxide deficiency. But,

the contribution of CO to vascular tonus of the thoracic aorta during aging and physical activity in rats is

not known. The purpose of this project is to observe any aging and swimming exercise associated

changes in the rat thoracic aorta

Materials and Methods: 4-month and 24-month old female rats were used and divided into four

following groups: sedentary young, trained young, sedentary old, and trained old. Swimming exercise

was performed for 8 weeks (60 min/day, 5 days/week). Thoracic Aorta isolated from the rats were

mounted on organ bath. Contraction responses of all vessel rings in presence and absence of HO

inhibitor (CrMP) were recorded as an endogenous CO contribution to vascular tonus. The effect of

exogenous CO relaxation response were assesed by CO releasing molecule (CORM).

Results: Although phenylephrine dose- response curves and Emax values with or without CrMP were

smilar in all groups, endogenous contribution of CO was higher in old group in comparison to that from

young controls. There was no differences in vasodilator CORM dose response curve reported for all

groups. However, in the old groups CORM Emax values were established significantly lower than that

the young groups (p<0,05).

Conclusion: As a result, The CO relaxation response decreases with aging in the thoracic aorta of

elderly rats and exercise is not an enhancing contributor for CO relaxation response.



O.14.Age-Related Changes In Type I Collagen And MMP-2 Expression in Mice

Mehmet Demir 1, 1Department of Anatomy, Faculty of Medicine, Kahramanmaras Sütcü Imam University,

Kahramanmaras, Turkey.

ABSTRACT

Introductions: The chewing system consists of several highly complex and interrelated structures. The

masticatory muscles, one of these structures, are the part of the stomatognathic system. The

masticatory muscles must be fully active for a physiologically and anatomically effective, painless and

less deformed chewing function.

Material and Method: Among the major effects of aging on the organism, the changes in the chewing

muscles and related disorders have become very important for clinicians. 14 Balb/c white mice (50-80

g) were used in this study. The animals were divided into two equal groups each containing seven

animals. Group I consisted of young animals (2-month-old) (n=7) and Group II consisted of old

animals (18-month-old) (n=7). After routine histological follow-ups were made, tissues were

embedded in paraffin. 4-5 μm-thick tissue sections were taken from the paraffin embedded tissues.

Then, they were stained immunohistochemically for aquaporin-1 and aquaporin-4 as well as with

hematoxylin-eosin.

Results: It was observed that age-related decline and shrinkage occurred in blood vessels. In young

mice, the masseter muscle tissue showed a weak immunoreactivity for type I collagen and MMP-2 and

the temporalis muscle tissue showed a moderate immunoreactivity for type I collagen and MMP-2. In

old mice, the masseter and temporalis muscle tissues showed a high immunoreactivity for type I

collagen and MMP-2 (p=0.001). In the H-score assessment, MMP-2 immunoreactivity was

significantly lower in young mice than in old mice (p=0.001).

Conclusions: Consequently, severe pain complications and functional losses are likely to occur due to

age-related degeneration of the masticatory muscles.

Keywords: Masticatory muscles, type I collagen, stomatognathic system, matrix metalloproteinase-2,

aging



O.15.An experimental study: Effect of acupuncture in wound healing

Mustafa ÇİÇEK 1, 1Department of Anatomy, Faculty of Medicine, Kahramanmaras Sütcü Imam University,

Kahramanmaras, Turkey.

Abstract

Introduction: Acupuncture regulates the process of inflammation and growth factors by increasing

blood circulation in the wound area. In this study, seconder open wound was created in rats, the effects

of acupuncture in acute (8 days) and chronic stage (25 days) were investigated.

Material and Method: Wound model in four groups (the acute period control group, the acute

period acupuncture group, the chronic period acupuncture group and the chronic period control group), a

total of 22 rats in the dorsal parts of secondary wound model was created with biopsy punch of 10mm

diamater.

Results: While total collagen density in the dermis of the control group was observed significantly

increased , animals of the acupuncture group was determined decreased collagen level (p=0,003).

Animals of the acupuncture group were seen significantly decreased in MMP-2 immunoreactivity of the

H-scores (p=0.02). Eventhough animals of the control group were observed significantly increased the

immune reactivity of type 1 collagen in the dermis, animals of the acupuncture group were revealed

modarately staining (p=0,008). In the twenty-fifth day conducted tensile strenght test animals of the

acupuncture group for necessary rupture force seperation of wound lips was greater than animals of the

control group (p<0,001

Conclusion: As a result of acupuncture treatment made a positive contribution to the wound

healing process with increasing of regeneration influence. Otherwise, less collagen density in the

acupuncture group has prevented keloid scar formation. Thanks to this situation, acupuncture has

brought to normal collagen connective tissue standards of collagen formation in the dermis.

Keywords: Wound, wound healing, acupuncture, type 1 collagen, MMP-2



O.16.EFFECTS OF LUCILIA SERICATA ON RAT CUTANEOUS WOUNDS HEALING:

REGULATION OF COLLAGEN I AND COLLAGEN III BY NF-KB (P65) ON DIABETIC

RATS

Fatma Kübra TOMBULTÜRK 1,2, Matem TUNÇDEMİR 1, Erdal POLAT 3, Serhat

SİREKBASAN 3,4, Gönül KANIGÜR-SULTUYBEK 1

1. Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey.

2. Medical Laboratory Techniques, Vocational School of Health Services, Istinye University, Turkey

3. Department of Medical Microbiology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul,

Turkey

4. Department of Biotherapy Research and Development Laboratory, Istanbul University, Istanbul,

Turkey

The impaired wound healing in diabetes mellitus is a major clinical problem. The use of Lucilia sericata

larvae on the healing of wounds in diabetics is reported. However, the role of the

excretion/secretion(ES) products of the larvae in treatment of diabetic wounds remains unknown. This

study investigated whether application on the wound surface of the ES products of L.sericata could

improve the impaired wound healing in Streptozotocin(STZ)-induced diabetic rats.

For this purpose, full–thickness skin wounds were created on the backs of STZ-induced diabetic rats and

the control group. A study was conducted to assess the levels of the ES-induced collagen-I and -III

expression and to assay NF-kB(p65) activity in ES-treated wound biopsies of STZ-diabetic rat skin in

compared to the control group. The expression levels of collagen I, III and NF-kB(p65) activity were

determined using immunohistological analyses and ELISA technique.

The results indicated that treatment with the ES increased collagen I expressions of the control and

diabetic wounds. But the increase in collagen I expression in the control group was higher than in the

diabetic group. NF-kB(p65) activity was also increased in diabetic wounds compare to the control

group. Whereas after treatment with ES, NF-kB(p65) activity was decreased in 3 and 7th days.

In conclusion, ES products of L.sericata may enhance the process of wound healing by influencing

phases such as inflammation, NF-kB(p65) activity, collagen synthesis and wound contraction. These

findings may provide new insight for understanding of therapeutic potential of ES in wound healing in

diabetics.



O.17.SYNTHESIS OF DISULFIDE BRIDGE BIS-DITHIAZOLEAMINE SCHIFF BASES

Cumhur KIRILMIS ve Osman KILINC

Adiyaman University, Faculty of Arts and Science, Department of Chemistry, Adıyaman

2-Aminothiazole unit is a crucial structural feature of significant classes of chemistry [1]. 2-

Aminothiazole and its derivatives have attracted a great deal of interest owing to their biological [2] and

psychotropic [3] activities. As medicines, many of them have a broad spectrum such as antibacterial,

antimicrobial, antifungal, anticancer and antitubercular activities [1,4].

On the other hand, Disulfides are observed in many organic structures having biochemical activity due

to S-S linkage which is characteristic of many system of biological interest [5,6].

In this study, 4,4'-disulfanediylbis(methylene)dithiazol-2-amine synthesized by the reaction of 1,3-

dichloroacetone with thiourea gave symmetrical disulfide bridge (R-S-S-R) linked to aromatic thiazole-

2-amine ring through methylene bridges. Schiff bases (2-10) were synthesized by reacting the starting

compound (1) with different aromatic aldehydes. All compounds were characterized by FTIR and 1H-

NMR techniques.

[1] D. Das, P. Sikdar, M. Bairagi, Eur. J. Med. Chem. 109 (2016) 89-98.

[2] P. Samadhiya, R. Sharma, S. K. Srivastava, S. D. Srivastava, J. Serb. Chem. Soc. 77 (2012) 599-605.

[3] S. I. Zav’yalov, O. V. Dorofeeva, E. E. Rumyantseva, L. B. Kulikova, G. I. Ezhova, N. E.

Kravchenko, A. G. Zavozin, Pharm. Chem. J. 35 (2001) 35-36.

[4] N. Singh, U. S. Sharma, N. Sutar, S. Kumar, U. K. Sharma, J. Chem. Pharm. Res. 2 (2010) 691-698.

[5] B. Mandal, B. Basu, RSC Adv. 4 (2014) 13854-13881.



O.18.INVESTIGATION OF IN VIVO PHARMACOKINETIC OF MICROCAPSULED

ROSEMARY ESSENTIAL OIL

İncilay GÖKBULUT a , Fatma Sezer ÖZTÜRK a, Burhan ATEŞ b, Hakan PARLAKPINAR c

a Inonu University, Faculty of Engineering, Department of Food Engineering Malatya

b Inonu University, Faculty of Arts and Sciences, Department of Chemistry Malatya

c Inonu University, Faculty of Medicine, Department of Pharmacology Malatya

e-mail:[email protected]

Microencapsulation is a technique that physically captures sensitive components in a protective

wall material to ensure that the core material or components are protected from adverse reactions,

volatile losses or nutritional deterioration. Rosemary (Rosmarinus Officinalis L.) is widely used

medically and aromaticly due to its essential oil properties belonging to the family Lamiaceae, and is a

plant that stands out with antibacterial, antimutagenic and antioxidant properties. The aim of this study

is to determine the in vivo pharmacokinetic properties of microcapsule rosemary essential oil.

In the scope of the study, the encapsulation of rosemary essential oils with alginate-starch as

coating material and the pharmacokinetics of essential oils in blood were carried out. Microcapsules

obtained by using rosemary essential oil (7% w / v) and alginate/starch (1%, 1% w / v) and free

rosemary essential oil were administered to experimental animals by gavage.

The release levels of essential oils were determined by taking the blood of the animals as dependent on

duration (at 30, 60, 120, and 240 min) after application of the free essential oil and microcapsule

essential oil. According to the results, it was determined that the free essential oils reached the highest

level in plasma at 30 min as 0.025 ± 0.002 mg EO/mL plasma. Essential oil was not detected in the

plasma samples taken after 60, 120 and 240 minutes in the application groups given free essential oil. In

the microcapsule essential oil treatment groups, essential oil was determined as 0.028 ± 0.003 mg

EO/mL plasma at 60 min. Essential oil could not be detected in plasma samples taken after 30, 120 and

240 minutes in microcapsule essential oil groups.

These results indicate that microcapsule essential oil samples reach the peak value later in the

plasma than free ones and prolong the release time of the capsulation process.



O.19.A selective fluorescent ‘turn-on’ sensor for recognition of Zn2+

in aqueous media

Mecit OZDEMIR

Department of Food Processing, Vocational High School, Kilis 7 Aralik University, Kilis, Turkey

E-mail: [email protected]

Recently, researchers have become increasingly interested in the design and development of fluorescent

sensors based on ion-induced changes (CIFA) in fluorescence due to these sensors’ highly selective and

sensitive recognition of biologically and environmentally crucial heavy and transition metal (HTM)

ions1. Zinc is the second most abundant transition ion in the human body with concentrations ranging

from nanomolar to millimolar2. It is an important cofactor in many biological processes such as neural-

signal transmissions and pathology, the regulation of metalloenzymes, gene transcription, immune

function, and mammalian reproduction. Besides that, the disorder of zinc metabolism in biological

systems has been correlated to epilepsy, diabetes, infantile diarrhoea and Alzheimer’s disease3.

Herein, we introduce a novel, selective fluorescent 'turn-on’ sensor ‘RhAP’ based on CIFA mechanism.

The RhAP chemosensor was prepared from a derivative of rhodamine B hydrazide and 2-acetylpyridine,

which was characterised using FT-IR, 13C NMR, 1H NMR, LC-MS/MS and elemental analysis. The

RhAP, a colorless and non-fluorescent compound, showed a selective fluorescent response and

colorimetric change for Zn2+ in HEPES buffered (10 mM, EtOH:water, 2:1, v/v, pH 7.2). However,

except for Co2+, Ni2+, and Cu2+ with little absorption, other commonly coexistent metal ions such as

Na+, K+, Ca2+, Mg2+, Cr3+, Fe3+, Al3+, Mn2+, Hg2+, Pb2+, Fe2+, Cd+2 and Ag+ did not introduce important

changes in color, absorption and fluorescence emission spectra. The complex formation between RhAP

and Zn2+ was found to have a 1:1 ratio. The results demonstrate that RhAP can be used as an effective

fluorescent sensor for selective detecting of Zn2+ in aqueous medium.

References:

1) Soh, J. H., Swamy, K. M. K., Kim, S. K., Kim, S., Tedrahedron Lett. 2007, 48, 5966.

2) Lu, D., Yang, L., Tian, Z., Wang, L., Zhang, J., RSC Advances, 2012, 2, 2783.

3) Xiang Long, H., Yang, L., Tao, L., Guo Ying, Z., Sci China Chem, vol. 57, No. 54, 2014.




O.20.SYNTHESIS, BIOLOGICAL AND THEORETICAL EVALUATIONS OF NOVEL

PIPERIDINE-HYDRAZONE DERIVATIVES

Nurcan KARAMAN1, Yusuf SICAK2,3, Tuğba TAŞKIN-TOK1, Emine Elçin ORUÇ-EMRE1,

Ayşegül KARAKÜÇÜK-İYIDOĞAN1, Mehmet ÖZTÜRK3

1Department of Chemistry, Faculty of Arts and Sciences, Gaziantep University, Gaziantep, Türkiye

2Department of Herbal and Animal Production, Köyceğiz Vocational School, Muğla Sıtkı Koçman

University, Muğla, Türkiye

3 Department of Chemistry, Faculty of Sciences, Muğla Sıtkı Koçman University, Muğla, Türkiye

Natural and synthetic antioxidants can be used for many chronic diseases and some researchers report

the relationship between antioxidant agents and anticholinesterase agents [1, 2]. Hydrazones and the

piperidine ring containing compounds have also important role in medicinal chemistry [3, 4]. According

to these knowledge, this study was aimed at the synthesis of two novel series of hydrazones derived

from ethyl 4-oxopiperidine-1-carboxylate and 2,6-diphenylpiperidin-4-one. Compounds were obtained

by condensing these piperidine containing ketones with benzoyl and benzene sulfonyl hydrazides [5].

Physical and chemical properties of compounds have been characterized and reported. The synthesized

compounds (1–36) were evaluated for their antioxidant capacity by using four complementary tests and

the anticholinesterase activities was performed against acetylcholinesterase (AChE) and

butrylcholinesterase (BChE) enzymes. In addition, cytotoxicity of the compounds was also screened.

The results showed that, most of the compounds displayed very good antioxidant activities and IC50

values of the compounds were better than or competed with the standard antioxidants'. Among these

compounds, compound 11 (IC50: 35.301.11 µM) inhibited BChE better than galantamine (IC50:

46.03±0.14 µM). We conclude that the compound 11 can be considered as a leader for BChE inhibitor.

Therefore, docking method was applied to elucidate the AChE and BChE inhibitory mechanism of the

compound 11. Molecular docking analysis revealed that compound 11 bound to BChE enzyme more

efficiently when compared to the AChE due to its orientations and different types of interactions. As a

result, with good activities, the non-cytotoxic properties of the compounds brought them into

prominence as a good candidate for anticholinesterase agents.

References

[1] H.S. Kareem, A. Ariffin, N. Nordin, T. Heidelberg, A. Abdul-Aziz, K.W. Kong, W.A. Yehye,

Correlation of antioxidant activities with theoretical studies for new hydrazone compounds bearing a

3,4,5-trimethoxy benzyl moiety, Eur. J. Med. Chem., 103 (2015) 497-505.

[2] S. Salla, R. Sunkara, S. Ogutu, L.T. Walker, M. Verghese, Antioxidant activity of papaya seed

extracts against H2O2 induced oxidative stress in HepG2 cells, LWT - Food Science and Technology, 66

(2016) 293-297.

[3] P. Parthiban, G. Aridoss, P. Rathika, V. Ramkumar, S. Kabilan, Synthesis, stereochemistry and

antimicrobial studies of novel oxime ethers of aza/diazabicycles, Bioorg. Med. Chem. Lett., 19 (2009)

6981-6985.

[4] S. Bala, G. Uppal, S. Kamboj, V. Saini, D.N. Prasad, Design, characterization, computational

studies, and pharmacological evaluation of substituted-N′-[(1E) substituted-

phenylmethylidene]benzohydrazide analogs, Med. Chem. Res., 22 (2012) 2755-2767.

[5] B. Koçyiğit-Kaymakçıoğlu, E. Elçin Oruç-Emre, S. Unsalan, S. Rollas, Antituberculosis activity of

hydrazones derived from 4-fluorobenzoic acid hydrazide, Med. Chem. Res., 18 (2008) 277-286.



O.21. THE ROLE OF FASUDIL TREATMENT ON AMYLOID BETA INDUCED

INFLAMMATION MODEL IN ASTROCYTES

Burçin Nilay YENER1, Hülya ÇİÇEK1

1University of Gaziantep, Medical Faculty, Department of Medical Biochemistry, Gaziantep/Turkey

Objectives: Alzheimer disease is a neurodegenerative disease that develops in millions of people all

over the world every year. Cytokines play a key role in inflammatory and anti-inflammatory processes

in Alzheimer’s disease. The common feature of neurodegenerative diseased brains is massive neuron

death due to oxidative stress. Fasudil, a rho kinase inhibitor that has neuroprotective effects. The aim of

this study is to investigate whether fasudil application will be a pharmacological approach to amyloid

beta-induced inflammation in astrocyte cell line.

Material and Methods: Astrocyte cells were incubated with 5 μM amyloid beta for 24 hours and

studied on two groups as control and amiloid beta. 2,5 μM fasudil was added to two groups for

treatment. cDNA synthesis was performed from RNA samples isolated from the cells. Gene expression

analysis was performed by real-time PCR method.

Results: IL-1β, TNF-α, Cas-3 and Cas-8 mRNA expression levels were 2-14 fold higher amyloid beta

than control group. Fasudil therapy significantly reduced the amyloid beta induced inflammation and

increase in some apoptotic genes. (p<0.001). Also, microscopic examinations showed the cell protective

effect of fasudil.

Conclusion: Earlier studies have shown that fasudil has protective effects against ischemic neuron

damage and inhibits production of TNF-α, IL-1β, Cas-3, Cas-8. As a result, the inhibition of rho kinase

by fasudil may be an agent that can be used in therapy with a protective effect on the suppression of

amyloid beta mediated inflammation. However, further work is needed to arrive at a definite conclusion.

Key Words: Alzheimer's disease, Amyloid beta, Fasudil, TNF-α, Cas-3, Cas-8



O.22.ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL ACTIVITY OF CRUDE EXTRACT

FROM SOME MEDICINAL PLANT ON FOUR PATHOGENIC BACTERIA

Badr Q. ISMAEL1, Hero M. ISMAEL2 and M. Hakkı ALMA3

1Graduate School of Natural and Applied Sciences, Bioengineering and Sciences, Kahramanmaraş

Sütçü İmam University, Avşar Campus, 46100, Kahramanmaraş, Turkiye

College of Science Biology Department, University of Salahaddin, Erbil, Iraq

2Faculty of Forestry, Forest Industry Engineering, Kahramanmaraş Sütçü İmam University, Avşar

Campus, 46100, Kahramanmaraş, Turkiye

ABSTRACT

Purpose: In this study, antioxidant activity and antimicrobial activity effects of chili pepper, dill, field

horse tail and thyme plant ethanol extracts on four pathogenic bacteria such as Klebsiella pneumoniae,

Staphloccocus aureus, Escherichia coli and Pseudomonas aerogenosa were investigated.

Method: The extracts were prepared from leaves and fruits for each plant by using microwave ethanol

extraction method. Some antibiotics, e.g., Rifamcin, Clindamycin, Vancomycin, Erythromycin,

Ciprofloxacin, Tobromycin, Crftazidime and Imipenem, were used as controls.

Findings: The results showed that the ethanol extract of thyme leaves and chili pepper had the highest

antibacterial activity compared to the other plant extracts mentioned above. However, synthetic

antibiotics just mentioned above were found to be more effective on bacteria in comparison with the

extract of thyme and chili pepper.

Discussion According to our finding indicated that the ethanol extract of these plants could be used

alone or together with conventional antibiotics to fight the against bacterial infection.

Key words: Antioxidant, total condense tannin plant extracts, antibiotic, pathogenic bacteria and

inhibition zone.



O.23.EFFECT ON BIOCHEMICAL PARAMETERS AND LIVER HISTOPATHOLOGY OF

MALATHION EXPOSURE NIGELLA SATIVA IN RATS

Haci Ahmet DEVECİ1, Gökhan NUR1, Merve ALPAY2, Hülya ÇİÇEK3

Akram Mudher Kareem ALJBORI 4 , Omar Mahmood Mohammed Mohammed4

1 Gaziantep University, Islahiye Vocational School, Department of Medical Services and

Techniques,Gaziantep

2 Düzce University, Faculty of Medicine, Medical Biochemistry Department, Düzce

3 Gaziantep University, Faculty of Medicine ,Department of Medical Biochemistry, Gaziantep

4 Gaziantep University, Department of Biochemistry and Technology, Gaziantep

Aim: The malathion (2- (dimethoxythiophosphorylthio) succinic acid diethyl ester) is a wide spectrum

organophosphate insecticide used in the agricultural sector to prevent harmful effects of pests on fruits

and vegetables. In this study, it was aimed to investigate the biochemical and histopathological effects

of donut oil in rats given malathion.

Materials And Methods: In our study, 24 Wistar albino rats, 8 rats in each group were used. 3 groups

were formed by feeding, 1.group 5 ml / kg of corn oil (control group), 2.group 50 mg / kg of malathion

+ Nigella Sativa oil and 3.groups of 5 ml / kg Nigella Sativa oil in which the rats were divided into 3

groups.Applications to all groups were maintained for 7 days. Animals were sacrificed by cervical

dislocation under ketamine / xylazine anesthesia and their blood was taken intracardially. Paraoxonase

(PON1) activity, malondialdehyde (MDA), total sialic acid (TSA) and nitric oxide (NO) levels were

measured in the obtained plasma samples. Liver tissue samples taken for histopathological examinations

were prepared after tissue tracing procedures and examined by light microscope.

Results:.In the experimental group applied malathion; Congestion, necrotic areas and cell infiltrations

were detected in the liver sections around the central and portal vein. Combination of malathion and

Nigella Sativa oil resulted in decreased frequency of findings and congestion and cell infiltration.

According to the results of biochemical measurements, plasma PON1 activity decreased, MDA, TSA

and NO levels increased in the malation group and it was found that these values were closer to the

control group in the donut-fed group.

Discussion and Conclusion: We believe that malathion, an organophosphate compound, enhances lipid

peroxidation and has a toxic effect on the liver, while Nigella sativa oil can be used as a preservative

against a malathion effects

Key words: Malathion, Malondialdehyde, Nitric oxide, Paraoxonase activity, Sialic acid



O.24.PRODUCTION, STORAGE AND PREPARATION TO USE IN LABORATORY OF

PLACENTA DERIVED MESENCHYMAL STEM CELLS AND ENDOTHELIAL CELLS FOR

SCIENTIFIC REASARCHES

Emin Turkay KORGUN, Muge MOLBAY

Akdeniz University, Medical Faculty, Department of Histology and Embryology

Akdeniz University Stem Cell Research and Application Unit, Antalya, Turkey

E-mail: [email protected] Tel ve fax: 242-2496885

Considering the possible treatment opportunities have been or will be promised, stem cell

therapy is expected to be answer for many uncured disorders. With their ability to differentiate into

various cell types and immune suppression, mesenchymal stem cells became a good candidate for cell

therapy and tissue regeneration. In this perspective, researchers of our country could not reach the

desired level in worldwide literature. Nowadays, many countries carry on cell therapy researches and

establish new facilities. When the cell types examined, mainly mesenchymal stem cells and bone

marrow derived mononuclear cells are used.

In scientific research studies besides to mesenchymal stem cells, other cell type frequently used

is endothelial cells. Both for research purposes and for clinical purposes, mesenchymal stem cells and

endothelial cells are used very often. Related with mesenchymal stem cells worldwide 37798, in Turkey

233 studies exist and related with endothelial cells 23657 of worldwide research and 176 of local

research have been done and in last five years, 5582 in whole world and 23 in Turkey have been

performed. Therefore, production of these cells in our country will contribute to Turkey originated

researches and provide an alternative for improvement of the competition of our country with others.

The aim of our study is isolation, characterization, culturing and freezing of the mesenchymal

stem cells and endothelial cells from human term placenta. Additionally, microbiological examinations

will be performed for preparation of them to researches.

In order to reach this goals, isolation cell culture, characterization with flow cytometry,

differentiation of the stem cells to cartilage, bone and fat cells have been performed. Besides,

characterization was confirmed with immunohistochemically techniques.




O.25.Antioxidant and Antigenotoxic Effect of Alpha Lipoic Acid in Obstructive Jaundice.

Tülay DİKEN ALLAHVERDİ1, Ertuğrul ALLAHVERDİ2, Pınar AKSU KILIÇLE3, Sevil

VURAL4

1Kafkas University Faculty of Medicine, Department of General Surgery, KARS

2Kafkas University Faculty of Medicine, Department of Orthopedics, KARS

3Kafkas University Faculty of Science and Literature, Department of Biology, KARS

4Ankara University Faculty of Veterinary Sciences, Department of Pathology, ANKARA

Aim: The aim of this study was to determine the antioxidant effect of intraperitoneal alpha lipoic acid

administration on obstructive jaundice in rats with experimental obstructive jaundice.

Material and Method: The study included 24 Wistar Albino rats distributed into three groups as Group I (Sham

group with laparotomy only, n=8), Group II with an obstructive jaundice model and intraperitoneal administration

of 25 mg/kg alpha lipoic acid (n=8), and Group III with an obstructive jaundice model only (control group, n=8).

Only median laparotomy was performed in Group I. The bile duct was ligated in both Group II and III. Group II

also received 25 mg/kg/day intraperitoneal alpha lipoic asid (a-LA) for 10 days. Blood samples were drawn on

postoperative day 3, 7 and 10 and the AST, ALT, GGT, ALP and Bilurubin levels measured at the biochemistry

laboratory. The rats were sacrificed at the end of day 10 and the liver SOD, GPX, and MDA levels were

determined. The femurs were removed for cytogenetic analysis where a micronucleus assay was performed.

Results: The bilirubin levels were 3.5 mg/dl in Group I and around 11.6 and 12.5 respectively in Group II and III

on day 3, 7 and 10.th bilirubin level above 5 mg/dl in rats indicates obstructive jaundice.The results showed that

AST, ALT, GGT, ALP and bilirubin levels were lower, SOD and GPX levels were higher, and the MDA level

was lower in Group II than in Group III. Administration of alpha lipoic acidsignificantly decreased the number of

micronuclei and had a protective effect on liver tissue by decreasing bile duct damage and liver inflammation,

fibrosis and necrosis.

Discussion: There was no difference between Group II and III for day 3 AST, GGT, ALP, T. Bilirubin and

D.Bilirubinlevels while levels of ALT, an enzyme indicating liver damage, were lower in Group II. The drug was

seen to start decreasing liver damage in the early period. Despite the lack of a significant difference between

Group II and Group III forT. Bilirubin, D. Bilirubin, AST, ALT, GGT and ALP levels on day 7, group II levels

were lower compared to Group III. On day 7, we observed that the drug had decreased liver damage and the

parameters that had increased with obstructive jaundice.

On day 10, there was a significant difference between Group II and III for AST, ALT and ALP levels while theT.

Bilirubin, D. Bilirubin and GGT levels were lower in Group II but with no significant difference. We therefore

found that the drug decreased liver damage by decreasing the parameters and liver enzymes that increase during

obstructive jaundice on day 10 as well as on day 7. There was a significant difference between the three groups

for levels of GSH and SOD, both substances with a hepatoprotective effect that decrease liver damage. We found

that the drug had a tendency to prevent liver damage by increasing these parameters and decreasing MDA

levels.There was a decrease in bile duct damage, polymorphonuclear leukocyte and lymphocyte infiltration,

necrosis and fibrosis in the alpha lipoic acid group compared to the control group. Our cytogenetic data showed a

significant difference between Group I, II and III for the number of micronuclei (p<0.001). Alpha lipoic acid had

a protective effect with a decreased number of micronuclei in Group II compared to Group III.

In conclusion, 25 mg intraperitoneal alpha lipoic acid administration seems to contribute to a decrease in

obstructive jaundice damage in rats with experimental obstructive jaundice.



O.26.GENE EXPRESSION ORIENTED DRUG REPOSITONING TARGETS FOR PROSTATE

CANCER

Beste TURANLI1,2, Gizem GULFIDAN2, Kazim Yalcin ARGA2

1Department of Bioengineering, Istanbul Medeniyet University, Istanbul, Turkey

2Department of Bioengineering, Marmara University, Istanbul, Turkey

The development of drugs against prostate cancer has utmost importance. On the other hand, drug

development is highly resource-intensive, time consuming and expensive. Drug repositioning, which

means utilization of an existing drug for treatment of a different disease, is a hot research topic. The

main objective is to repurpose drugs for prostate cancer via a novel framework utilizing systems

biomedicine and pharmacology tools and perspective.

A comprehensive gene expression dataset (GSE6919) was downloaded from Gene Expression Omnibus

(GEO) database to distinguish gene expression profiles originating from 192 cancer and 232 healthy

samples. Differential expressed genes (DEGs) were analyzed through application of RMA normalization

and LIMMA method via R/Bioconductor software (v.2.12). P-value threshold (p<0.01), and fold

changes (FC) were used to determine up (FC>1.2) and down-regulated (FC<0.83) genes. DEGs were

analyzed thru DGIdb 2.0 to acquire gene-drug interactions. P-values were calculated for drugs in the

dataset via employment of a hypothesis test based on hypergeometric distribution.

As a result, 688 DEGs comprising 484 down-regulated and 204 up-regulated genes were used to

determine drug interactions. 118 of them have been associated with 522 drugs and 595 gene-drug

interactions were acquired thru the proposed method. The method has revealed Bicalutamide,

Cabazitaxel, Docetaxel, Flutamidec which are already used for treatment and also Metformin, Digoxin

which are repurposed drugs for prostate cancer, recently. Candidates which have not been previously

associated with prostate cancer are also examined in detail as drug targets and will be evaluated in silico

and in vitro in the future works.



O.27.Preparation and Characterization of Antiviral and Anticancer Drug Printed Film

Formulations for the Treatment of Cervical Cancer

Cem VARAN1, Murat ŞEN2, Niklas Sandler3, Erem BİLENSOY 1, 4

1 Department of Nanotechnology and Nanomedicine, Hacettepe University, Ankara, Turkey

2 Department of Chemistry, Hacettepe University, Ankara, Turkey

3 Pharmaceutical Sciences Laboratory, Åbo Akademi University, Turku, Finland

4 Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara,

Turkey

Aim: The aim of this study was to develop and investigate pharmaceutical and mechanical properties of

anticancer and antiviral drugs printed bioadhesive film formulation for the cancer treatment.

Method: Two different ink formulations containing anticancer drug-cyclodextrin inclusion complex or

antiviral drug-loaded polycaprolactone nanoparticles were prepared and printed on hydroxypropyl

cellulose film by using an inkjet printer. Drug amount, release profile and mechanical properties of film

formulations and in vitro cell culture studies were realized.

Results and Discussion: Complete release of anticancer drug was reached within a period of 8h and

antiviral drug was reached within 16h with a slower rate from film formulations. Mechanical

characterization tests and cell culture studies showed that developed film formulations are safety,

effective and stable. The amount of drug in the film can be personally adjusted with this approach.

Conclusion: Our study proves that inkjet printing of anticancer and antiviral drugs on film seem to be a

potential drug delivery system for cervical cancer treatment. This film formulation is a good candidate

for personalized medical applications.

Acknowledgement: Financial support by TUBITAK (Project Number: 114S793). Cem Varan is a

recipient of grant from TUBITAK BIDEB (2211-C).



O.28.EVALUATION OF CHOLESTEROL-TARGETED AMPHİPHİLİC CYCLODEXTRİN

NANOPARTİCLES FOR CANCER THERAPY

Gamze VARAN, Selin ÖNCÜL, Ayşe ERCAN, Juan M. BENITO, Carmen Ortiz MELLET, Erem

BİLENSOY

Department of Nanotechnology and Nanomedicine, Hacettepe University, Ankara, Turkey Department

of Biochemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey Institute for Chemical

Research, CSIC-University of Sevilla, Sevilla, Spain Department of Organic Chemistry, Faculty of

Chemistry, University of Sevilla, Sevilla, Spain Department of Pharmaceutical Technology, Faculty of

Pharmacy, Hacettepe University, Ankara, Turkey

The aim of this study was to determine the effects of surface charge of amphiphilic cyclodextrin

nanoparticles on interaction with cancer cell membrane and evaluate the intrinsic apoptotic effect of

blank nanoparticles.

Anionic (6ocaproβcd) and cationic (pcβcdc6) amphiphilic cyclodextrin nanoparticles were used in this

study. Nanoparticles were characterized by in vitro and the apoptotic effects of nanoparticles were

demonstrated by caspase-8 activity, lipid peroxidation, tunel, cholesterol assay and gene expression

studies.

Blank nanoparticles have cytotoxicity against a variety of cancer cells but none to healthy cells. Blank

nanoparticles were found to be effective on cell colonization and membrane integrity in apoptosis

studies. In conclusion, blank anionic and cationic amphiphilic cyclodextrin nanoparticles induced

apoptosis through mitochondrial pathway targeted to cholesterol microdomains in cancer cell

membrane.

Amphiphilic cyclodextrins are favorable nano-sized systems without anticancer agent for cancer

therapy. Therefore, apoptotic effects of cyclodextrin derivatives should be taken into consideration for

cancer treatment.

Acknowledgement: financial support by tubıtak (project number: 112s538).

Gamze Varan is a recipient of grant from TÜBİTAK BİDEB (2211-c)



O.29.Preparing Cardiac-Implantable Electrophysiological Device Coated with Biocompatible,

Antibacterial Polyurethane Films Against the Post-Implantation Infection Problem

Merve Gökşin KARAASLAN1, Emre BİRHANLI2, M. Buğra KARAASLAN3 Süleyman

KÖYTEPE1, Burhan ATEŞ1

3 Cukurova University, Medical Faculty, Department of Cardiology, Adana, TURKEY

[email protected]

The use of implantable electrophysiological devices in cardiac surgeons has increased significantly

with technological advances. However, the increasing number of treatments in the incidence of cardiac-

implantable electrophysiological devices (CIEDs) infections has also increased following implantation [1].

These infections cause prolonged hospitalization, increased mortality and increased cost of treatment [2].

For this reason, in order to reduce the risk of infection, it is very important to be made up of antibacterial

forms of CIEDs.

In this contexts, the aims of this study are to develop polyurethane films that do not contain any

antibiotics but are structurally antibacterial, to coat CIED with these films and to examine their

antibacterial properties. In the study, 4,4′-methylenebis(cyclohexyl isocyanate), polyethylene glycol 200

(PEG 200), tween 20 and boric acid containing biocompatible polyurethanes were synthesized (PU-T20-

BAs). The synthesized boric acid-based polyurethane materials are structurally defined using a

combination of FTIR, TGA, DTA and DSC analysis techniques. The antibacterial properties of

polyurethane materials were examined on Escherichia coli (ATCC® 25922 ™) and Staphylococcus

aureus (ATCC® 25923 ™). When both bacteria’s results are examined, it was determined that the

synthesized PU-T20-BAs showed high antibacterial properties and the polymer films had zone

diameters varying between 25-35 mm. In addition, it has been examined that the effects on the both

bacteria species of partially coated the commercial CIEDs by using polyurethane material which has

antibacterial properties. As a result, it has been determined that polyurethane materials which have been

prepared for the problem of infection developing after CIEDs implantation are safe materials.

Referances

[1] DZ Uslan, IM Tleyjeh, LM Baddour et al. Am Heart J (2008) 155, 896–903.

[2] G Marsch, B Mashaqi, K Burgwitz et al Europace (2014) 16, 604–611.




O.30.Investigation of BSA Solutions Depending on Temperature by NMR

Gülten Kavak Balcı

Dicle University, Department of Physics, Diyarbakır, 21280 TURKEY

The importance of understanding the interactions of water with globular proteins has been investigated by several

authors using mainly two different approaches and presenting some methods. The first approach is based on the

analysis of NMR T1 and T2 relaxation mechanisms in protein solutions.

In this study, spin-lattice relaxation process in two concentrations of several BSA solutions has been studied at

temperatures from 233K to 304K. Relaxation measurements were carried out on a Vnmr (Varian NMR )

spectrometer operating at 300 MHz for proton. It was seen that NMR relaxation measurements (T1) have a

dependence on the temperature of the sample. The 1/T1 relaxation rates increase as the protein concentration

increases, and decrease with increasing temperature.

These results were correlated well with the results from other measurements techniques.This implies that the

protein is reducing the motion and temperature increasing motion of water molecules. Spin-lattice relaxation

rates 1/T1, were fitted versus 1/T. The activation energies and correlation times (c ) were calculated by using the

experimental data and relaxation theory related. The least-squares fitting of LnT1 versus 1/T gives a linear

relationship, and the data suggest that the relaxation mechanism of BSA is caused by a fast chemical exchange of

water molecules between protein-bound water and free water.

Referances

1. Ekinci, A, Köylü, MZ, Askin, M (2014), Temperature Dependence Study of Chiral Ether Derivative by 1H

NMR Relaxation Time, Asian Journal of Chemistry; Vol. 26, No. 8 2400-2402

2. Van-Quynh, A., Wilson,S. and Bryant, R.G. 2003. Protein reorientation and bound water molecules

measured by 1h magnetic spin_lattice relaxation, Biophysical Journal, 558-563.

Mp/Mw=0,2417

y = -1544x + 5,6828

R2 = 0,9908

-0,4

00,4

0,8

0,0032 0,0034 0,0036 0,0038 0,004

1/T(K-1)

lnT1(s)

Mp/Mw=1,2788

y = -1451,7x + 4,5026

R2 = 0,9435

-2

-1,5

-1

-0,5

0

0,0032 0,0034 0,0036 0,0038 0,004 0,0042

1/T(K-1)

LnT1(s)

http://search.proquest.com/indexinglinkhandler/sng/au/Ekinci,+Arzu/$N?accountid=15780

http://search.proquest.com/indexinglinkhandler/sng/au/K$f6yl$fc,+M+Zafer/$N?accountid=15780

http://search.proquest.com/indexinglinkhandler/sng/au/Askin,+Muzaffer/$N?accountid=15780



O.31.Beta-Fibrinogen -455 G/A and Angiotensin Converting Enzyme I/D Gene Polymorphisms in

Patients with Lung Cancer

Hamit Hakan ALP1, Fatma Zuhal UMUDUM2, Ebubekir BAKAN2

1: Department of Biochemistry, Faculty of Medicine, Yüzüncü Yıl University, Van, Turkey

2: Department of Biochemistry, Faculty of Medicine, Atatürk University, Erzurum, Turkey

Abstract

Background/aim: Lung cancer is one of the most common and high mortality-rated cancer type.

Fibrinogen is a plasma protein which has a role in the coagulation process. It is thought that fibrinogen

has role in growth, angiogenesis and metastasis of tumor. Angiotensin-converting enzyme (ACE) is an

important regulator of blood pressure and cardiovascular homeostasis. In our study, we have

investigated β-fibrinogen -455 G/A and ACE I/D polymorphisms in lung cancer patients and healthy

volunteers. In this study, we aimed to determine relation between these polymorphisms and lung cancer.

In addition, we aimed to clarify whether reason of disorder observed in fibrinogen and ACE synthesis in

patients with lung cancer is those polymorphisms or not.

Materials and Methods: Samples in our study was obtained from 100 patients with lung cancer

and 100 healthy volunteers. Plasma fibrinogen levels were measured with ELISA method.

Polymorphism analyses were determined with PCR-reverse hybridization method.

Results: Results of our study reveal that fibrinogen levels of patients with lung cancer were

significantly higher than health control group (p<0.01). No difference was determined for β-fibrinogen -

455 G/A and ACE I/D polymorphisms between patients with lung cancer and healthy control group. In

both groups fibrinogen level was higher in persons bearing AA genotype compared to persons bearing

GG and GA genotypes in both groups (p<0.05)

Discussion: As a result, it can be stated that β-fibrinogen -455 G/A and ACE I/D polymorphisms

are not related with formation and prognosis of lung cancer.

Keywords: Lung cancer, ACE I/D, β-fibrinogen -455 G/A, polymorphism



O.32.INVESTIGATION OF THE EFFECTS OF SOME ANTICANCER AGENTS ON THE ACTIVITY

OF MITOCHONDRIAL THIOREDOXIN REDUCTASE AND CYTOSOLIC GLUTATHIONE S-

TRANSFERASE BY PURIFICATION, KINETIC AND MOLECULAR DOCKINS STUDIES

Ilknur OZGENCLIa,b, Deryanur KILICb,d, Mehmet CIFTCIc, Omer Irfan KUFREVIOGLUb, Harun

BUDAKa

aDepartment of Molecular Biology and Genetics, Science Faculty, Ataturk University,

Erzurum,Turkey

bDepartment of Chemisrty, Science Faculty, Ataturk University, Erzurum,Turkey

cDepartment of Chemisrty, Art and Science Faculty, Bingol University, Bingol,Turkey

dDepartment of Chemisrty, Art and Science Faculty, Aksaray University, Aksaray,Turkey

INTRODUCTION

While thioredoxin reductase (TrxR) plays an important role in the regulation of intracellular redox balance and

various signalling pathways, glutathione S-transferase (GSTs) enzymes belong to detoxification family that

catalyse the conjugation of glutathione with various endogenous and xenobiotic electrophiles. It is known that

TrxR is overexpressed in many aggressive tumors and led to tumor growth and progression in cancer cells. GSTs

detoxify xenobiotics from the cells thus provide resistance to chemotherapeutic anticancer drugs Therefore, they

have been identified as potential target to develop chemotherapeutic strategies.

METHODS

TrxR2 and GST were purified from rat liver having a specific activity of 0.436, 1640.930 EU/mg proteins with a

yield of 39.2%, 31.280% and 207.6, 3516.584 of purification fold, respectively. After the purification procedure,

in vitro inhibition effect of anticancer drugs (cisplatin, calcium folinate, carboplatin, epirubicin hydrochloride,

doxorubicin hydrochloride, paclitaxel, etoposide, fluorouracil, and methotrexate) on both enzymes was

investigated. Furthermore, molecular docking study was performed to determine the binding site and binding

affinity of methotrexate to both rat TrxR2 and MGSTA1-1.

RESULTS AND DISCUSSION

TrxR2 was strongly inhibited by all of the anticancer drugs with the IC50 values of 0.29, 4.00, 4.70, 7.80, 8.30,

10.00, 48.00, 66.00, and 970 µM, respectively. GST was not inhibited by the anticancer drugs apart from

methotrexate with 3.64 mM IC50 value. Finally, both enzymes were inhibited by only methotrexate in rat liver,

and methotrexate was well placed in the active sites of both proteins. In terms of the data obtained from this

study, methotrexate drug tends to develop less resistance during chemotherapy and it can be said that it is the best

among the anticancer agents used in this study. Our findings are thought to be useful for the development of new

chemotherapeutic strategies.



O.33.Screening of antioxidant-anticancer activity of Mentha longifolia (L).) Hudson subsp

typhoides (Brig) var. typhoides on HeLa cancer cells

Önder YUMRUTAŞ1, Mustafa PEHLİVAN2, İbrahim BOZGEYİK1, M. Özgür ÇEVİK4, Ahmet

ARSLAN5

1University of Adiyaman, Faculty of Medicine, Department of Medical Biology, Adiyaman, Turkey 2University of Gaziantep, Vocational School of Nurdağı, Department of Medicinal and Aromatic Plants,

Gaziantep, Turkey 3University of Adıyaman, Faculty of Medicine, Department of Medical Genetic, Adiyaman, Turkey 4University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey

Aim: In this study, we aimed to determine anticancer activities of apolar, semipolar and polar extracts

of Mentha longifolia on HeLa cancer cells

Methods: Mentha longifolia plant powders were subjected to standard Soxhlet extraction and hexane,

dichloromethane and methanol extracts were prepared. Cell viability was assessed by MTT assay and

active doses were determined. The effects of extracts on induction of apoptosis and cell cycle were

determined by using Annexin V/propodium iodine (PI) staining. Also, DNA fragmentation, ROS

induction and morphological changes were analyzed after extract treatments. In addition, cellular

antioxidant activities were determined by fluorescence spectroscopy. Changes in expression levels of

apoptotic, tumor suppressor, antioxidant, DNA repair, cell cycle genes were determined.

Findings: DCM and MeOH extracts is found to be induced antiproliferative activity in a dose-dependent

manner. The DCM extract induced apoptosis by 21.5%, but the MeOH extract did not induce apoptosis.

Also, in cell cycle analysis cells are found to be accumulated in aneuploid G1. In DNA fragmentation,

smear formation was observed after extract treatments. Also, DCM extract was increased intracellular

ROS content. Also, membrane shrinking and apoptotic bleb formation was observed. Lastly, GPX4,

AIFM1, CIDEP, DDR1, CDK2, SMAD1 gene differentially expressed.

Discussion: In the light of these findings, DCM extract of Mentha longifolia were found to have anti-

cancer activities by inducing apoptosis in HeLA cervix cancer cells and can be used in the future cancer

treatments.

Key words: Anticancer, apoptosis, cell cycle, intracellular ROS, gene expression, Mentha longifolia

Acknowledgement: This research was funded by TUBITAK with the project number 112S572.



O.34.STRUCTURAL INSIGHTS INTO AMYGDALIN AS A MULTI-FUNCTIONAL ANTI-

CANCER AGENT

Khattab AL-KHAFAJIa, Tuğba TASKIN TOKa

aGaziantep University, Faculty of Arts and Sciences, Department of Chemistry, 27310, Sehitkamil,

Gaziantep, Türkiye.

E-mail [email protected]

The main aim is to investigate the ability of amygdalin to work as a multi-functional anti-cancer agent.

Therefore, we are focusing to study the inhibition activity of natural ligand and interactions between

amygdalin and proteins (CDK1, AKT, and BCL-2) by using flexible ligand docking approach of

AutoDock 4.0.

The docking study results showed that amygdalin is a potential inhibitor of all the three protein targets,

due to minimum binding energies (-5.73, -5.10 and -3.14 kcal/mol) and Ki (4.99 mM, 63.32 uM and

182.64 uM) values of the complexes. As a conclusion, our study reveals that amygdalin offers the

strength to inhibit mentioned proteins and which can consider fighting cancer in different positions.

References

1.Liyu Qia, Bo Xie, Yaguo Wang, Jun Qian. (2015). International Journal of Clinical and Experimental

Pathology, 8 (5), 5363-5370.

2.Chen, Y., Ma, J., Wang, F., Hu, J., Cui, A., Wei, C., Yang, Q. and Li, F. (2012).

Immunopharmacology and Immunotoxicology, 35(1), pp.43-51.

3.Juengel, E., Thomas, A., Rutz, J., Makarevic, J., Tsaur, I., Nelson, K., Haferkamp, A. and Blaheta, R.

(2015). International Journal of Molecular Medicine, 37, pp.526-532.

4.Forli, S. et al., 2016.. Nature Protocols, 11(5), pp.905–919.



O.35.EXOSOME BASED TREATMENTS AS A NEW METHOD FOR THE TREATMENT OF

CANCER

Gülper NACARKAYHA1, İsmail KAŞOĞLU2, Füsun TUŞGÜL1, Mustafa YILDIRIM3

1: Gaziantep University, Medicine Faculty, Department of Medical Biology.

2: Yeditepe University, Graduate School of Natural and Applied Sciences

3: Bahcesehir University, Department of Internal Medicine

Aim

Exosomes carry a variety of cargo of proteins and genetic information to regulate

morphogenesis, growth, differentiation and adult tissue homeostasis, creating spatial and temporal

gradients through the extracellular environment of adjacent tissues after released from the cells which

they are produced. The aim of this review is to summarize current developments in the therapeutic use

of exosomes which draws more attention in recent years in cancer studies.

Exosomes and Cancer

Exosomes have been isolated from the cell culture medium and in many body fluids such as

blood, plasma, serum, urine, bronchial fluid, pleural effusion, synovial fluid, ocular fluid, cerebrospinal

fluid, breast milk and saliva. For this reason, it’s easy to get exosomes, it has come to the fore to be a

biomarker and treatment target that exosomes can use early in cancer diagnosis. Exosomal miRNAs are

functional and can silence target cell genes. The roles of these miRNAs also demonstrated in

immunoreactivity, viral infections and endothelial cell migration, prometastatic immune response, and T

cell suppression.

Exosomes carry information from the donor cells to the recipient cells. Thus it leads to genetic

and phenotypic changes in the recipient cell. It has been shown that mRNAs isolated from exosomes

obtained from colorectal cancer cells are involved in tumor angiogenesis. Glioblastoma origined

exosomes have been found to be loaded with proteins that increase angiogenesis and mRNA expression

from the cells they are derived.

Discussion

The development of cell-free exosomal based therapies are exciting applications. Besides,

rearrangement of exosomal surface molecules allows targeted use of therapeutic agents for more

specific organ or malignant cell types. This protects other organs and cells from potential side effects of

treatment. We believe that exosomal based therapies are promising in the treatment of cancer.



O.36.Micro-RNA Profile of Childhood Acute Leukemia

Barbaros Şahin Karagün

Aims: Micro-RNAs are functional, non-protein coding RNA molecules and their transcriptions provided

by intron or exon regions of the genome and non-protein coding regions of RNA genes. In this study we

aimed to identify micro-RNA profiles in the childhood and adult acute leukemia that one of hematologic

malignancies.

Materials and Methods: 37 acute leukemia patients (31 children diagnosed with ALL and 6 children

diagnosed with AML ) who admitted to Çukurova University, Medical Faculty, Department of Pediatric

Hematology between Dec 2010 and Sep 2011 were included in study. Control group was formed with

47 healthy children. MicroRNA analysis was performed to plasma samples with PCR.

Results: Newly diagnosed pediatric patients compared with healthy control groups; miR20a, miR25,

miR92a, miR30c, miR106b, miR203, miR150, miR192, miR302c, miR184, miR218, miR320, miR342-

3p, miR223, miR328, miR483-5p, miR376a, miR381, miR451, miR576-3p, miR548a levels were

increased in newly diagnosed patients according to healthy control groups (p <0.05). The miR20b,

miR342-3p and miR548a levels in healthy control group of patients were increased according to newly

diagnosed group (p <0.05 ) Healthy control groups compared with child patients whose in remission;

miR769-3p, miR20a, miR92a, miR16, miR27b, miR192, miR320, miR223, miR484, miR451 levels

were increased in healthy control groups according to child patients whose in remission.

Result: This study has shown that, by identifying miRNAs that have different level of expression

between normal and pathological tissues, new miRNAs, that are effective in diagnosis and treatunent of

human concers, may be determined.



O.37.Two Wavelength High Intensity Irradiation for the Effective Crosslinking of DNA to Protein

Emine YAVUZ1,2, Craig David FAIRCHILD2

(1) Selcuk University Advanced Technology Research and Application Center, Selcuklu, Konya;

(2) Worcester Polytechnic Institute, MA, USA

Protein-DNA crosslinking is an important method to study protein-DNA interactions in biomedical

applications especially signalling pathway studies. Crosslinking by short pulsed UV lasers is a

potentially powerful tool that results in efficient crosslinking, apparently by a two photon process.

However, the major problem in using UV laser crosslinking method is the conditions which lead to high

crosslinking efficiency also result in high DNA damage. The objective of this study is to develop a

novel UV laser crosslinking technique that would permit higher effective crosslinking yield with the

commonly used pulses in the nanosecond (ns) range.

We used MBP-PIF3 (phytochrome interacting factor-3) protein (expressed in E. Coli from a constructed

plasmid and purified by affinity chromatography technique) and its target G-box DNA motif

(radioactivelly labeled) as a model system. After expression, purification and immunoblotting studies

were completed the specific binding interaction of purified MBP-PIF3 protein with the G-box DNA

motif was studied by Electrophoretic Mobility Shift Assay (EMSA). We irradiated the PIF3/DNA

complexes with different laser systems (i.e. Nd:YAG and Dye lasers) and their combinations.

We found that after UV laser irradiation the amount of full-length product was almost 2/3 of that from

the unirradiated DNA whereas upon low intensity irradiation we could not detect any amplified DNA.

Our data showed that high powered UV laser pulses damage DNA less than conventional UV

irradiation. This new approach has the potential to be tested on a different DNA-binding protein (i.e.

transcription factors) that has a greater propensity to undergo UV-promoted DNA crosslinking.



O.38.Application of 1H NMR-Based Metabolomics for Toxicological Evaluation

Ozlem DEMIRCI1*, Nurcan DOGAN BINGOLBALI2, Murat KIZIL3, Pelin UGURLU4

1Science Faculty, Department of Biology, Dicle University, Turkey 2Gebze Institute of Technology, Gebze/Kocaeli, Turkey 3Science Faculty, Department of Chemistry, Dicle University, Turkey 4Science and Technology Application and Research Center, Dicle University, Turkey

[email protected]

The aim of the study: Glyphosate is currently the top-selling pesticides in the world and its selling

keeps on the grow correspondingly increase in the cultivation of glyphosate-tolerant (GT) transgenic

crops. As seen with other pesticides, glyphosate, due to its high water solubility, may contaminate

surface and ground waters. In the present study, a 1H nuclear magnetic resonance (NMR) based

metabolomics approach was applied to investigate the toxicity of glyphosate in Cyprinion macrostomus.

Material and Methods: The commercial formulation of glyphosate (Roundup) supplied from a local

agricultural pest store, were used. Static renewal acute toxicity tests were performed in glass dishes

containing 5 L test solution with 10 fish/vessel. We used 3250 µg/L and 6500 µg/L (the amount of

residual water that previously determined mean 650 ng/L) as study concentrations for 96-h.

Results: We determined the exposure of Cyprinion macrostomus to Glyphosate-based herbicide

disrupted metabolism and resulting in significant changes in metabolite levels in the whole-body as

identified by 1H nuclear magnetic resonance (NMR) based metabolomics.

Discussion: Detected metabolic changes indicate that glyphosate causes significant toxicity in the

organism.

Keywords: Glyphosate, Metabolomic, 1H NMR




O.39.SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITIES OF

Ag (I) -N-HETEROCYCLIC CARBENE COMPLEXES

Öznur DOĞAN ULUa, İlknur ÖZDEMİRb, Selami GÜNALc, Nevin GÜRBÜZa,b,

İsmail ÖZDEMİRa,b

aCatalysis Research and Application Center, Inönü University, Malatya, Turkey bFaculty of Science,Department of Chemistry, Inonu University, Malatya, Turkey

cFaculty of Pharmacy, Department of Microbiology, Inonu University, Malatya, Turkey

N-Heterocyclic carbenes (NHC) have been widely used in organometallic chemistry, catalysis and

medicinal chemistry by the introduction by Ofele and Wanzlick in 1968 and then the first stable carbene

synthesized by Arduengo in 1991. Due to their advantages such as the strong σ-donor properties, π-

backbone ability and stable to air and moisture relative to similar ligands, the stable complexes with

main groups and transition metals have been synthesized.

The design of new Ag-NHC complexes has received considerable interest in recent years [1]. The

anticancer, antibacterial and antimicrobial properties of different Ag-NHC complexes constitute a field

for medicinal applications as well as synthesis of new metal complexes via transmetallation provides an

effective application area in organometallic chemistry.

Therefore, we synthesized several Ag(I)-NHC complexes and characterized via different spectroscopic

techniques in this work. In addition, the antimicrobial activity these new Ag (I) -NHC complexes were

evaluated against gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria and gram-

positive (Staphylococcus aureus and Enterococcus faecalis) bacteria and two fungal strains (Candida

albicans and Candida tropicali). The results indicated that the synthesized Ag-NHC complexes

exhibited better activity compared to positive antimicrobial control.

Keywords: N-heterocyclic carbene, Ag-NHC, antimicrobial activity

REFERENCES:

[1] Liu Y., Wang Z Shi J., Chen B., Zhao Z., Chen Z., J. Org. Chem. 80, 12733−12739,2015.

[2]Muhammad A., Muhammad A. I., Mouayed A. H., Chern Ein O., Rosenani A. H., Mohamed B.K.A.,

Aman S.A.M., Amin Malik S.A.M. Eur. J. of Med. Chem. 108,177-187,2016.

[3]Achar G., Shahini C.R., Patil S.A., Budagumpi S., J. of Organomet. Chem. 833, 28-47,2017.

[4] Sakamoto R., Morozumi S., Yanagawa Y., Toyama M., Takayama A., Kasuga N.C., Nomiya K. J. of

Inorg. Biochem. 163,110-117,2016.



O.40.INHIBITION PROPERTIES OF SOME FLAVONOIDS ON CARBONIC ANHYDRASE I,

AND II ISOENZYMES PURIFIED FROM HUMAN ERYTHROCYTES

Zübeyir HUYUT1, Şükrü BEYDEMİR2, İlhami GÜLÇİN3

1Department of Biochemistry, Medical Faculty, Yuzuncu Yıl University, Van, Turkey

2Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey 3Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, Turkey

Aim: Carbonic anhydrases (CAs, E.C.4.2.1.1) are plays a critical role in many important physiological

events and treatment of some diseases.1 Flavonoids or phenolic compounds have been discovered as

novel CAs inhibitors instead of the traditional sulfonamides, with different binding to CAs, pro-drug

activities and new inhibition mechanisms.2 In this study, we investigated the inhibition effects of some

flavonoids including Malvin, Callistephin, Oenin, Pelargonin, Silychristin and 1-(4-Methoxyphenyl)-2-

methyl-3-nitro-1-H-indol-6-ol (ID-8) against esterase and CO2-hydratase activities of hCA I and hCA II.

Method: hCA I and hCA II were purified from human erythrocytes (RBCs) by Sepharose-4B-L-

tyrosine-sulphanilamide affinity column chromatography. The purity of the isoenzymes was checked by

sodium-dodecyl-sulfate-gel-electrophoresis (SDS-PAGE) according to the Laemmli’s method.3 The

esterase and CO2-hydratase activities of hCA isoenzymes were measured according to the methods

defined by Verpoorte et al. and Rickly et al, respectively.4-6

Results: Both hCA isoenzymes were inhibited by flavonoids, with IC50 and Ki values in the range of 2.3

nM - 346.5 µM and 51.0 - 99.6 µM for hCA I, and 86.6 - 750.0 µM for hCA II, respectively. Malvin

and Oenin with IC50 values of 2.3 nM - 99.0 μM and 18.7 - 0.35 μM, respectively, exhibited more

effective inhibition profile according to the other used substances on hCA I and II.

Discussion: These results showed that flavonoids especially Malvin and Oenin effectively inhibited

hCA I, and II isoenzymes. Hence, they may be used as an effective CA inhibitor in medical applications

for treatment of certain diseases such as glaucoma, in the future.

Key words: Antioxidants; Carbonic anhydrase; Enzyme inhibition; Flavonoid; RBCs

References

1. Huyut Z, Beydemir Ş, Gülçin İ. Inhibitory effects of some phenolic compounds on the activities of carbonic anhydrase:

from in vivo to ex vivo. Journal of Enzyme Inhibition and Medicinal Chemistry, 2016; 31(6): 1234-1240.

2. Innocenti A, Gülçin I, Scozzafava A, Supuran CT, Bioorganic and Medicinal Chemistry Letters, 2010; 20: 5050-5053.

3. Laemmli, DK. Cleavage of structural proteins during in assembly of the head of Bacteriophage T4. Nature, 1970; 227: 680.

4. Verpoorte JA, Mehta S, Edsall JT. Esterase activities of human carbonic anhydrases B and C. Journal of Biological

Chemistry, 1967; 242: 4221-4229.

5. Rickly EE, Ghazanfar SAS, Gibbons BH, Edsall JT. Carbonic anhydrase from human erythrocytes, Journal of Biological and Chemistry,

1964; 239: 1065-1078.

6. Wilbur KM and Anderson NG. Electrometric and colorimetric determination of carbonic anhydrase. Journal of Biological

Chemistry, 1948; 176: 147-154.

http://www.tandfonline.com/author/Huyut%2C+Z%C3%BCbeyir

http://www.tandfonline.com/author/Beydemir%2C+%C5%9E%C3%BCkr%C3%BC

http://www.tandfonline.com/author/G%C3%BCl%C3%A7in%2C+%C4%B0lhami



O.41.A facile synthesis of Amide Based Receptors Under Microwave Conditions: Investigation of

Their Anion Recognition Properties by Experimental and Computational Tools

Gülsen Öztürk, Salih Subari, Selami Ercan, Necmettin Pirinççioğlu

Dicle University, Department of Chemistry, Diyarbakır, 21280 TÜRKİYE

The significant role of anions in many chemical and biological areas is well-described. Therefore, the

development of anion sensors and chemosensors has drawn attention in the last years and become an

important sub-area within the field of anion chemistry.

Two novel amides were synthesized under microwave irradiation. The chemical structures of amides

were confirmed by means of IR, 1H NMR, 13C NMR, and elemental analysis. Their binding properties

for various anions (H2PO4-, HSO4

-, C6H5CO2-, CH3CO2

-, ClO4-, F-, Cl- and Br-) were examined by UV

titration in THF at 20 oC. Molecular dynamic calculations by AMBER provide models for the

complexation mode between the receptors and anions with detailed binding and quantum mechanic

calculations at B3LYP and M062X levels using 6-31+g(d,p) basis sets produce binding energies for the

complexes.

Referances

1. Mong MW, Xie H, Kwa ST (2013) Anion recognition byazophenol thio urea-based chromogenic

sensors: a combined DFT and molecular Dynamics investigation. J Mol Model 2013 19(1):205-13.



O.42.Suitability of Magnetic Fe3O4 Nanoparticles in Hyperthermia Treatment

K.O.YAKINCI1, T. DEPCİ1, S. SAYIN3, M.E.YAKINCI2

1 İskenderun Teknik Üniversitesi, Mühendislik Fakültesi, Mühendislik Temel Bilimleri Bölümü, Merkez

Kampüs, 31200-İskenderun, Hatay. 2 İskenderun Teknik Üniversitesi, Teknoloji Fakültesi, Malzeme ve Metalürji Mühendisliği Bölümü,

Merkez Kampüs, 31200-İskenderun, Hatay. 3 İskenderun Teknik Üniversitesi, Deniz Bilimleri ve Teknolojisi Fakültesi, İskenderun, Hatay.

Nowadays, developments in nanotechnology can easily be transferred to biomedical and health

sectors with different modifications.As a consequence, one of the most successful issuesis the use of

magnetic Fe3O4 nanoparticles in cancer therapy with the aid of different coupling fluids in less time than

anticipated, and recently many positive results can be shown successfully.In a manner, when magnetic

nanoparticles exposed to a varying magnetic field, they are heated due magnetic hysteresis losses and

then created a locally heated environment in the regions where they are present. So the temperature to be

created in the local section can be reached up to 41-440C. It is thought that warmed tumor cells are

degraded at 41-42 °C so if Fe3O4 nanoparticles to be used in the treatment of local cancer cells and

important studies have been done internationally in this regard and today it continues rapidly.

Today, it has been found that Fe3O4 nanoparticles which 2-10 nm in size are good tools for

cancer treatments and clinical applications. However, their production is often difficult and laborious

and great efforts are need to achieve the proper properties.In this study, production of magnetic Fe3O4

nanoparticles was carried out by using chemical precipitation method and the effects on physical and

magnetic properties were investigated. It was found that,for superparamagnetic Fe3O4

nanoparticles,when Fe2+/Fe3+ ratio decrease 50%,the particle size is found to decrease by about one

fold.A general characterization of the Fe3O4 particles, produced at about 10 nm in size, for the

applications in the biomedical sector has been made and its usefulness in the treatment of hyperthermia

has been discussed.

Key Words: Fe3O4, nanoparticles, biomedical, hyperthermia



O.43.DNA Damaging Activities of Some PTP1B Inhibitors

Şafak OZHAN KOCAKAYA, Necmettin PIRINCCOĞLU

University of Dicle,Faculty of Science, ChemistryDepartment, Diyarbakır

Cancer is a versatile genetic disease, affecting a large number of populations with a very high cost world

wide. One of the most important goals in medicinal chemistry is the development of new heterocyclic

compounds with antitumor activity. Over the past several decades, researchers have been working to

understand the genetic events leading to tumorigenesis and also to accomulate information which may

lead to develop effective treatments.

The present study reports docking and moleculr dynamic calculations for the complexes of

dodecanucleotide d(CGCGAATTGGCG)2 with four ligands, already known as protein thyrosine

phosphate 1 B (PTP1B) enzyme inhibitors. The complexes were prepared by docking these molecules

into the dodecanucleotide and their interaction modes were investigated by molecular dynamic (MD)

calculatios. The results produced very significant outcome, which may be used in the design and

development of new anti cancer agents.

References:

1. Gilson MK, Zhou HX Calculation of protein-ligand binding affinities. Annu Rev

BiophysBiomolStruct 36, 21–42, 2007.



O.44.GENE EXPRESSİON OF ADAMTS1 ON HUVEC EXPOSE TO CİGARATTE SMOKE

GULSEN Filiz1, GEYIKLI CIMENCI Iclal2, Khandakar A. S. M. SAADAT3

1 Gaziantep University Institute for Health Sciences Department of Medical Biochemistry,Gaziantep,

Turkey

2 Gaziantep University Faculty of Medicine Department of Biochemsitry, Gaziantep, Turkey

3 Gaziantep University Faculty of Medicine Department of Medical Biology, Gaziantep, Turkey

ABSTRACT

It is known that cigarette smoke induces and accelerates the atherosclerotic process in the endothelium.

Studies have demonstrated the key role of extracellular matrix degradative proteases in atherosclerosis.

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs), a member of the matrix

metalloproteinase family (zinc-dependent matrix enzymes) acts by splitting structural proteins of

extracellular matrix such as collagen, versican and aggrecan. Versican is found in all three layers of

developing blood vessels and arterial walls. ADAMTS1, a member of ADAMTS family, can cleave

versican. Versican is up regulated in all vascular disease forms. In addition, versican has been found to

accumulate in different arterial lesions, including restenotic lesions and atherosclerotic plaques. The

ability of ADAMTS1 to cleave the proteoglycan versican is likely to be central to the hypothesized roles

for the proteases in atherosclerosis. It is reported that hypoxic condition in endothelial cells increased

ADAMTS1 gene expression, induced by hypoxia inducible factor (HIF-1). New findings demonstrated

that cigarette smoke induced HIF activation too. However, how cigarette smoke affects gene expression

of ADAMTS1 has not yet been elucidated.The aim of this study is to determine the effect of cigarette

smoke on the mRNA expression level of ADAMTS1 which have the ability to degrade the versican on

the human umbilical vein endothelial cell line (HUVEC) model. HUVEC cells were incubated with 1%

Cigarette Smoke Extract (CSE) for 4 hours and 6 hours. HUVEC without cigarette smoke were used as

controls. Quantitative Real-Time PCR (qRT-PCR) was performed using primers targeting the

ADAMTS1 gene. GAPDH was used as internal control. Previously ADAMTS1 mRNA expression

pattern at hypoxic HUVEC was reported.The expression was increased at early hypoxia and decreased

late hypoxia time courses. Our results for CSE is smiliar this data. We have shown that, the mRNA

expression of ADAMTS1 increased 5 fold at 4h CSE (p <0.05) and 2,5 fold in 6h CSE(p <0.05).These

data provides information on effect of cigarette smoke on gene expression of ADAMTS1 and may

contribute to studies on the role of ADAMTS1 in atherosclerosis.

Keywords: Cigarette Smoke Extract (CSE), Atherosclerosis, ADAMTS1, Gene Expression Real Time

PCR



O.45.EFFICIENCY OF VOLUME REDUCTION BY APPLICATION OF ADDITIONAL

LOWER LEVEL TOURNIQUET IN SHORT TERM LOWER EXTREMITY OPERATIONS

Ergün MENDEŞ1, Elzem ŞEN1, Mehmet CESUR1, Sıtkı GÖKSU1, Hüseyin GÖÇERGİL1 1University of Gaziantep, School of Medicine, Department of Anesthesiology and Reanimation,

Gaziantep, Turkey

Aim: Intravenous Regional Anesthesia (IVRA) in lower extremity is not widely used due to high dose

and volumetric risks. We aimed to investigate the effectiveness of IVRA with less volume by applying a

short-duration additional tourniquet from a lower level.

Methods: The study included retrospective review of 40 ASA I-II-III adult patients who had underwent

elective foot and ankle surgery. Patients were divided into two groups: Group I (n=20) received 200 mg

lidocaine hydrochloride completed to 20 ml with saline and Group II (n=20) received 200 mg lidocaine

hydrochloride completed to 30 ml with saline. Periods of tourniquet pain formation, perioperative and

postoperative hemodynamic parameters and VAS values were compared.

Results: Demographic data were similar in both groups. In Group 1, tourniquet pain was found in total

time of 35.75 min while group 2 was in total 38.8 min.There was no significant difference in VAS

values in all time periods between the two groups.

Discussion: In our study, effective anesthesia was provided in all groups and no side effects were

encountered.We believe that an additional lower level tourniqet and low-volume IVRA can be applied

to reduce unwanted side effects for short-duration lower extremity surgeries.



O.46.REGIONAL ANESTHESIA IN CLAVLICULA OPERATIONS

Azı̇z Yarbı̇l1, Necla Benlier2Ali Bestemi Kepekçi3, İbrahim Halil Özcan4, Sevda Mecit1, Hasan

Kanadlı1, Orhan Kılıç1, Alkan Karakış1, Mehmet Çalık5

1 Kilis State Hospital Department of Anesthesiology and Reanimation, Kilis

2SANKO University Medical Faculty Medical Pharmacology Department, Gaziantep 3Istanbul New Century University SHMYO Department of Anesthesia, Istanbul 4Private Academy Hospital Department of Anesthesiology and Reanimation, Gaziantep 5Department of Anesthesiology and Reanimation, Tarsus State Hospital, Mersin

Aim: In our study; We aimed to determine whether the regional anesthesia method used in clavicular

fracture operations reduces the need for analgesia in these patients and whether they are discharged

early.

Maternal & Methods: Anesthesia chart of Eight-two patients who have undergo clavlicula fracture

operation between April 2014-October 2016 with regional anesthesia (RA) were reviewed. Methods of

RA, age, gender, operation admission (suffered trauma or war victim),and complications were

retrospective analyzed. Patients divided 3 groups according to their age; 0-16 ages classified as

pediatrics group,17-65 ages classified as adults group and above 65 ages classified as geriatrics group.

Admission of operation was compared war victim or trauma.

Findings: Cervical block was performed to all patient’s interscalene brachial plexus (ISB)+deep C4

level location. After 0.25%bupivacaine 0.5%lidocain mixture was prepared. The prepared mixture was

administrated ISB(15cc) + C4(5cc)to pediatric group , ISB(30cc) + C4(10cc) to adult group; ISB(20cc)

+ C4(10cc) to geriatric group under sedation with ultrasound guidance. Approximately 15 minutes later

operation was started.

82 patients’ vital sing was stable during the operations. Neither analgesia nor additional anesthesia were

need during 78 patients operation. Additional anesthesia and analgesia were needed during soft tissue

removing towards to breast.

Discussion and Results: Rejyonal anesthesia has the known advantages such as reducing the use of

postoperative analgesia, reducing hospital costs and early discharge. According to our knowledge

clavlicula surgery was performed with RA at a limited number center and there are different opinions

about blockage. ISB combined with superficial cervical blockage is most common approach. We found

that C4 deep cervical blockage combined with ISB has effective results for clavlicula surgeries. We

couldn’t find larger case series than ours in literature. In our opinion this study will shed light on future

studies. We suggest that ISB + C4 deep cervical blockage can use safely and effectively in patients who

suffered trauma or war injury.

Key words: Clavicular Fracture, Regional anesthesia



O.47.NEXT GENERATION SEQUENCING REVEALS ASSOCIATION OF C.2461G>A IN

COL1A1 GENE WITH OSTEOGENESIS IMPERFECTA: A CASE REPORT

Naser GILANI1,2, Rozhgar A. KHAILANY1,3, Ahmet ARSLAN4, Khandakar A. S. M. SAADAT1,,

Amir ARIAMAND3, Mohammad R. ABBASZADEGAN5

1Department of Medical Biology, Health Science Institute, Faculty of Medicine, Gaziantep University,

Gaziantep, Turkey; 2Farabi Medical Laboratory, Erbil, Iraq; 3Department of Biology, College of

Science, Salahaddin University, Erbil, Iraq; 4Department of Medical Biology, Faculty of Medicine,

Namık Kemal University, Tekirdag, Turkey; 5Medical Genetic Research Center, School of Medicine,

Mashhad University of Medical Sciences, Mashhad, Iran.

Purpose: Osteogenesis Imperfecta (OI) is a range of disorders mostly affect the bones leading to

multiple fractures especially in extremities due to mild trauma or even spontaneously. Other clinical

features like short stature, hearing loss and abnormal sclera could be seen. According to the types of OI

severity differs from prenatal-lethal to approximately symptom free picture. Mutations in the COL1A1

and COL1A2 genes are responsible for more than 90% OI cases. The case we are going to discuss is a

newly married young couple with an affected 1-month-female neonate. There is history of suspicious

ultrasonography report as abnormal lesions in femur in prenatal screening, as her parent say. She is

suffering from numerous fractures in her different bones and extremities` deformity.

Method: On molecular investigation, we screened proband COL1A1, COL1A2 and CRTAP genes using

Illumina HiSeq4000 platform.

Findings: Mutation c.2461G>A (p.Gly821Ser; Het) in COL1A1 gene was detected. However, no

mutation was detected in COL1A2 and CRTAP genes.

Discussion: This genetic defect alters structure of collagen type I protein and subsequently weakens

connective tissues, particularly bones, resulting in the clinical features of osteogenesis imperfecta, which

is consistent with our proband`s sign and symptoms. COL1A1-related OI is inherited in an autosomal

dominant manner, however the noticeable proportion of cases caused by a de novo mutation. In our case

on the basis of her clinical picture, healthy parents and negative family history it seems detected

mutation should be a de novo one. At last, we suggested to validate the mutation by Sanger sequencing

in proband and parents, and prenatal testing in further pregnancies.

Keywords: Osteogenesis Imperfecta, COL1A1, Next Generation Sequencing.

https://www.ncbi.nlm.nih.gov/books/n/gene/glossary/def-item/autosomal-dominant/

https://www.ncbi.nlm.nih.gov/books/n/gene/glossary/def-item/autosomal-dominant/



O.48.A SINGLE NUCLEOTIDE POLYMORPHISM OF SUR1 GENE DETECTED IN TYPE 2

DIABETIC PATIENTS LIVING IN ERBIL PROVINCE-IRAQ

Rozhgar A. KHAILANY1,2, Naser GILANI1,3, Belan O. KANABE4, Ahmet ARSLAN5 and

Khandakar A. S. M. SAADAT1, Kaifee ARMAN1

1Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey; 2Department of Biology, College of Science, Salahaddin University, Erbil, Iraq; 3Farabi Medical

Laboratory, Erbil, Iraq; 4Department of Biology, Gaziantep University, Gaziantep, Turkey; 5Department

of Medical Biology, Faculty of Medicine, Namık Kemal University, Tekirdag, Turkey

Purpose: Type 2 diabetes mellitus (T2DM) is a polygenic disorder characterized by defects in insulin

secretion and peripheral insulin resistance. Genome wide screening and segregation analysis of T2DM

DNA has revealed a substantial susceptibility loci suggested the genetic heterogeneity that affects the

gene-environment interactions that determine the interplay balance of insulin, glucagon and the

somatostatin hormones. Once this balance of interplay disrupted, variety of endocrine disease arise

including T2DM. A correlation was suggested between SNPs in the Sulfonylurea receptor 1 (SUR1)

gene and T2DM in different populations. The SUR1 gene encodes the SUR1 protein that plays a key

role in glucose-induced insulin secretion. We aimed to investigate AGG1273AGA single nucleotide

polymorphism of the SUR1 gene in association with T2DM in Erbil population.

Method: The study included 190 samples; 92 control and 98 patient samples. Genomic DNA was

extracted from blood samples, the polymorphism was analyzed by restriction fragment length

polymorphism-polymerase chain reaction (RFLP-PCR) technique.

Findings As a result of gene polymorphism analysis, we observed an increase in A allele frequency of

SUR1 gene in T2DM patients compared with control group.

Discussion: According to our finding there was a significant association between the SNP and

increasing risk of T2DM (p<0.012) in our target population.

Keywords: Type 2 DM, Polymorphism, RFLP-PCR.



O.49.Synthesis of Novel Hydrazone Derivatives and Investigation of Their Biological Activities

Samir Abbas Ali NOMA1, Merve Goksin KARAASLAN1, Sevgi BALCIOGLU1, Tuncay TUNC2,

Burhan ATES1, Mahmut ERZENGİN2

1Inonu University, Faculty of Science and Arts, Department of Chemistry, Malatya, Turkey 2Aksaray University, Faculty of Science and Arts, Department of Chemistry, Aksaray, Turkey

[email protected]

Hydrazones are Schiff bases compounds formed by condensation of a hydrazine with a ketone or

aldehyde [1]. In recent years, hydrazones have been intensively investigated mostly because of their

potential biological application as anticancer, antiviral, antibacterial, and antifungal agents [1-3]. For

this reason, we synthesized different hydrazone derivatives (Figure1) and their structures were

characterized by XRD, H-NMR, FTIR and elemental analysis. Then synthesized compounds were

subjected to screening for their antioxidant activities by DPPH, ABTS and reducing power method.

When the synthesized substances subjected to antioxidant activities, the heights antioxidant activity

according to DPPH and reducing power methods was 27.7 ± 0.005 mg trolox equivalent/100 mg sample

and 35.00±0.016 mg trolox equivalent /100 mg sample value, showed by compound 2, respectively. By

contrast, according to the ABTS method, it was determined that the best antioxidant activity was found

in compound 1 with 164.04±0.009 mg trolox equivalent/100 mg sample value. The inhibitory effect on

purified hPON1 of hydrazone compounds were evaluated by in vitro and IC50 value range were from

0.0185-0.0353 mg/mL. In addition, their anticancer activities were evaluated by MTT test on breast

cancer cell line MCF-7.

Referances

1. Jones, R.D., Summerville, D.A. and Basolo, F., 1979. Synthetic oxygen carriers related to

biological systems. Chemical Reviews, 79(2), 139-179.

2. Olie, G.H. and Olive, S., 1984. The Chemistry of the Catalyzes hydrogenation of carbon

Monoxide, Springer, Berlin, Germany.

3. Dugas, H. and Penney, C., 1981. Bioorganic Chemistry, Springer, New York, USA.




O.50.Development of Haemostatic Matrixs Based on Polyurethane Prepolymer and Biological

Applications

Sevgi Balcioglu1, Suleyman Koytepe1, Burhan Ates1

1Inonu University, Faculty of Science, Department of Chemistry, Malatya, Turkey

Biomaterials that do not leak blood and biological fluids are now widely using in today's

technology. In particular, cyanoacrylate based systems for external tissues and hydrogel based systems

for internal tissues are used. In these systems, especially in terms of their use in vessels, blood sealing

and the ability to withstand certain pressures are very important. In addition to these primary properties,

properties such as flexibility are also important to adapt to the movement of the organism [1]. Although

hydrogel-based systems have a high level of flexibility, their mechanical strengths are weaker because

they absorb water. Due to their low flexibility, cyanoacrylate based systems also have limited use,

especially in internal tissues. For this reason, there is a need for systems that have both high flexibility

and high mechanical strength and which can be gelled quickly.

In our system, monomer and time optimization were carried out for fluidity of prepolymer by

using 4,4′-methylenebis(cyclohexyl isocyanate) and PEG (200, 600). Then aqueous solutions were

prepared at specific concentrations for alginate, chondroitin sulphate, carboxymethylcellulose and

chitosan (10%, 5%, 2.5%, 1%, 0.5%, 0.25% w/v). Prepolymer and polysaccharide units were mixed and

gelation times were determined. For 0.5% and 1% concentrations of alginate, the gelation time was

determined to be 10 min (Figure 1). For the samples with the shortest gelation times, aluminum and soft

tissue adhesion test after gelation, AFM analysis before and after gelation, measuring of Tg by DSC for

determination of flexibility and in vitro biocompatibility tests were performed.

Figure 1. Gelation time of alginate-prepolymer system; A, E represents 0,25%; B, F represents 0,5%; C,

G represents 1%; D,H represents 2,5% alginate-prepolymers. A, B, C, D represent the gelation image of

0th minute and E, F, G, H represent the gelation image of 10th minute.

References : 1. McDermott, M. K., Chen, T., Williams, C. M., Markley, K. M., & Payne, G. F. (2004).

Mechanical properties of biomimetic tissue adhesive based on the microbial transglutaminase-catalyzed

crosslinking of gelatin. Biomacromolecules, 5(4), 1270-1279.



Poster Presentation

Abstracts for the SANKO University Innovation in Medicine Summit-III

11th-13th May 2017, Gaziantep, Turkey



P.1.Next-Genetarion Drug Design as PTP1B Inhibitors

Şafak OZHAN KOCAKAYA1, Mehmet KARAKAPLAN1, Rosario SCOPELLİTİ2

1University of Dicle, Faculty of Science, Chemistry Department, Diyarbakır 2 Ecole Polytecnıcque Federale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland

The small molecule design that can inhibit the protein tyrosine phosphatase (PTP1B) enzyme is an area

of research that has considerably large prescription in the medicinal field that is actively pursued by

many academic and industrial organizations. Is a negative regulator for insulin receptor signaling of the

enzyme PTP1B and is a suitable drug target for the treatment of insulin resistance associated with

diabetes and obesity. In clinical trials, the association between insulin resistance and PTP1B levels in fat

and muscle tissues has been determined. Studies on mice have shown that insulin sensitivity and obesity

increase in PTP1B enzyme deficiency.

The aim of this study is synthesis and understanding of the role of insulin resistance of novel computer-

aided inhibitors for Protein Tyrosine Phosphatase 1B enzyme which is important therapeutic target as a

negative regulator of insulin hormone on the basis of today's extremely widespread and untreated

diseases such as Type II Diabetes and obesity. Therefore, chiral 1 and 2 molecules were synthesized

from (1R,2R)-1,2-diaminocyclohexane and identified by spectroscopic and X-Ray crystallography

methods. Potential inhibitor effects of these molecules to the target protein were investigated by dock

and MD calculations. At the same time, the interactions with the enzyme-activated insulin receptor have

been detailed.

1 2

References:

1. Zhang, Z.-Y. and Lee, S.-Y PTP1B inhibitors as potential therapeutics in the treatment of type 2

diabetes and obesity. Expert Opin. Investig. Drugs 12,223–233, 2003.

2. Bialy, L. and Waldmann, H. Inhibitors of protein tyrosine phosphatases:next-generation drugs.

Angew. Chem. Int. Ed. Engl. 44, 3814–3839, 2005.



P.2.IMMOBILIZATION OF L-ASPARAGINASE ON PHEMA/STARCH BIOCOMPOSITE: A

RECOVERABLE BIOCATALYST FOR L-ASPARAGINE REMOVAL

Ahmet ULU1, Süleyman KÖYTEPE 1, Burhan ATEŞ 1

1Inonu University, Faculty of Science & Art, Department of Chemistry, Malatya, Turkey

([email protected])

L-asparaginase is an indispensable chemotherapeutic drug in the treatment of leukemia (especially for

childhood leukemia). However, this treatment is an expensive process. The basic principle of the

treatment is to remove the L-asparagine which is necessary for tumor cells [1]. Therefore, there is a need

for novel designs that can be use for more efficient use of the enzyme and removal of L-asparagine. The

aim of this work is to design a new column system that can be used to remove L-asparagine from

biological fluids with biocompatible carrier matrix containing immobilized enzyme.

Herein, PHEMA composite activated with starch were used as packing materials in a fixed-bed column.

The PHEMA-5% starch was selected as column packing material because of highest immobilization

efficiency. Then, the column packing material was characterized by a variety of technique including

structural, thermal and morphological. The L-asparagine solution was loaded onto the column and the

level of L-asparagine in fractions taken at certain times was determined. The same procedure was

repeated with column material without L-asparaginase.

Experimental result showed that 95% L-asparagin removal efficiency is achieved. These findings

suggested that PHEMA/starch composites in the column structure presents a great potential in removal

of L-asparagine.

Finally, this study has potential to direct new approaches with its originality, practicality and end results.

References:

[1]. Zhang, Y. Q.; Tao, M. L.; Shen, W. D.; Zhou, Y. Z.; Ding, Y.; Ma, Y.; Zhou, W. L. Immobilization

of L-asparaginase on the microparticles of the natural silk sericin protein and its characters. biomaterials

2004, 25, 3751−3759.




P.3.RESULTS OF KARYOTYPE ANALYSİS OF 7766 PREGNANCIES PRENETALY IDENTIFIED

WITH AMNIOCENTESIS IN TURKEY

Ayfer PAZARBAŞI1, Osman DEMİRHAN1, Ümit LÜLEYAP1, Lütfiye ÖZPAK1,

Seda N. ILGAZ1, I. Nur USLU1, Gamze CÖMERTPAY1, Gülsevinç AY1,

Sabriye Kocatürk-Sel1, Nilgün TANRIVERDİ1, Selim Büyükkurt2

1. Department of Medical Biology, Faculty of Medicine, Çukurova University, Balcali, Adana, Turkey

2. Department of Obstetrics and Gynecology, Faculty of Medicine, Çukurova University, Balcali,

Adana, Turkey

Purpose: Prenatal diagnosis of fetal chromosomal abnormalities is the most common indication for

invasive prenatal testing. Amniocentesis is a very crucial diagnostic procedure for preventing the birth

of genetically defective fetuses in order to decrease the prevalence of genetic diseases in populations.

Method: A retrospective review of our amniocentesis database for the period from January 2000 to

April 2017 was carried out. The karyotyping of 7766 fetuses was carried out in Department of Medical

Biology from the samples of amniotic fluids which were sent from Department of Gynecology and

Obstetrics of Balcali Hospital. A standart nomenclature has been developed to describe each of types of

abnormality found in human chromosomes.

Results: A total of 7766 amniocentesis specimens were processed during the study period. 516 fetuses

(6.64%) had various chromosomal abnormalities. 56.78% of abnormal karyotypes (293 cases) were

numerical and 41.08% (212 cases) were structural. Both numerical and structural chromosomal

aberrations were observed in 11 cases (2.13%). The numerical abnormalities were as: trisomy 21

(45.39%), trisomy 18 (18.08%), monosomy X (10.58%), trisomy 13 (7.16%), Triploidy (5.46%),

Klinefelter Syndrome (3.75%), Trisomy X (1.36%), XYY Syndrome (1.02%), and the others. The

frequent structural abnormalities were as: 46,XX/XY,inv(9)(p11;q12)/(p11;q13) (35.21%),

46,XX/XY,1qh(+)(9.43%), 46,XX/XY,16qh(+)(4.71%), 46,XY,Yqh(-) (4.24%),

46,XX/XY,9qh(+)(3.77%) and 46,XY,Yqh(+) (3.30%). Balanced and unbalanced translocations,

deletions and duplications were also found.

Conclusions: According to the literature and our results, advanced maternal age is the main cause of

fetal chromosomal abnormalities. Fetal chromosomal abnormality ratio that we found was 6.64%. This

ratio emphasize the importance of prenatal diagnosis.

Key Words: Chromosomal abnormality; Cytogenetic, Prenatal diagnosis, Indication, Advanced

maternal age.



P.4.Detection of the maternal 833T ˃C and 844ins68 polymorphisms of Cystathionine beta

synthase gene and risk of Down syndrome offspring

Ayfer Pazarbaşı1, Sabriye Kocatürk-Sel1, Lütfiye Özpak1, Nurşen Keser1, Umit Luleyap1, Selim

Büyükkurt2

1Department of Medical Biology, Faculty of Medicine, 2Department of Obstetrics and Gynecology,

Faculty of Medicine, Çukurova University, Balcali, Adana, Turkey.

INNOVATION IN MEDICINE MEETINGS 2, May 5-7, 2016 / The University of Sanko, GAZİANTEP

INTRODUCTION: Down syndrome (DS) is the most common trisomy in live born with a prevalence

of 1 in 1000 to 1 in 1100. Cystathionine beta synthase (CBS) gene located on chromosome 21

synthesises cystathionine from homocysteine and serine. We have looked for an association of

polymorphisms in the CBS gene and the risk of birth of a child with Down syndrome.

MATERIALS AND METHODS: In our study, sixty-sevens DS mothers and fifty-three mothers who

had no children with DS from Çukurova region of Turkey were evaluated. The CBS genotypes were

studied by PCR-amplified products.

FINDINGS AND RESULTS: Among controls, the genotypes of A1A1 and A1A2 were observed in

81% and 19%, respectively, whereas the A1A1 and A1A2 genotypes were observed in 84% and 16% of

case patients, respectively for CBS 844ins68 polymorphism. The results showed that the frequencies of

CBS alleles (A1 and A2), as well as the frequencies of CBS 844ins68 genotypes (A1A1 and A1A2) do

not correlate with DS pregnancies, demonstrating no difference between the case and control groups.

BsrI digestion products of heterozygous for CBS 844ins68/833T˃C (A1A2) were 248 bp (for insert allele

A2) and 180 bp (for noninsert allele A1). All heterozygous for CBS 844ins68/833T˃C (A1A2) of case

patients were at CC genotype for T833C polymorphism.

DISCUSSION: In the present study, we did not find any statistically significant association between

CBS 844ins68 polymorphism and history of DS pregnancies. It seems likely that T833C polymorphism

plays a role on births of individuals with Down syndrome.

Keywords: Down syndrome, Maternal polymorphisms, Cystathionine beta synthase

This study was supported by Cukurova University Scientific Research Projects Unit (Adana, Turkey)

with TF2014BAP1 number.



P.5.Frequencies and distributions of sex chromosome abnormalities in females with Turner

phenotype; the long-term retrospective study in South Region of Turkey

Nilgün Tanrıverdia, Osman Demirhana*, Dilara Süleymanovaa, Ayfer Pazarbasia

aDepartment of Medical Biology and Genetics, Faculty of Medicine, Çukurova University, Balcali-

Adana/Turkey

INNOVATION IN MEDICINE MEETINGS 3, May 11-13, 2017/ The University of Sanko,

GAZİANTEP

Background/Aims: The genetic background of Turner syndrome (TS) is highly variable. The

correlation between genotype and phenotype is not yet well understood. The aim of this study was to

describe the frequencies and distributions of Turner karyotypes and to discuss the phenotype-genotype

relation in very large group of individuals with TS.

Materials and Methods: The karyotype results of 248 female participants were evaluated,

retrospectively.

Results: 14.5% of 248 females with Turner phenotype had normal karyotype and 85.5% had Turner

karyotypes. About 72.2% of the abnormalities were numerical aberrations, and 27.8% were structural

aberrations. The most important findings are as following: The most frequent karyotype was monosomy

X, which was found in 135 females (63.7%), followed by 44 mosaics (21%), 40 isochromosome of the

long and short arms of chromosome X (19.1%), and 17 deletions of the short and long arms of

chromosome X (8.0%). One case with robertsonian translocation and one case mosaic Turner with

marker chromosome were detected.

Conclusion: This study is showing the frequency and distribution of karyotypes in TS females. The

great value to be gleaned from studies of TS females in furthering our understanding of the atypical

clinical features associated with TS. Studies involving genetic analyses will be necessary to examine

gene expression profiles in girls with TS and identify potential candidate genes underlying the atypical

clinical features associated with TS.

Key words; Turner syndrome, monosomy X, turner fenotype



P.6.CYTOTOXICITY OF GAMBOGIC ACID ON NTERA-2 AND NCCIT TESTICULAR

CANCER CELLS 1Aysegul HANIKOGLU, 2Ferhat HANIKOGLU, 3Aysegul CORT, 1Tomris OZBEN

1 Akdeniz University Faculty of Medicine Department of Medical Biochemistry, Antalya, Turkey

2 Kemer State Hospital, Medical Biochemistry Laboratory, Antalya, Turkey 3 Sanko University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Gaziantep,

Turkey

Objectives

Although testicular cancer is rare, it is the most common solid organ tumor between the ages of 18-38.

Gambogic acid (GA) exhibits anti-cancer activity on various cellular pathways. Our study of testicular

cancer cell lines with the Ntera-2 and NCCIT cells was conducted to determine the sensitivity to GA.

Materials and methods Ntera-2 and NCCIT cells were obtained from the American Type Culture Collection (ATCC). The

cytotoxicity of both cells to GA was investigated by using MTT test, Ntera-2 and NCCIT cells were

incubated with different concentrations of GA (0.25 mg/L, 0.5 mg/L, 0.75 mg/L and 1 mg/L) for 24

hours.

Results We demonstrated that GA had cytotoxic effect on both testicular cells. Incubation with GA decreased

the cell viabilities of Ntera-2 and NCCIT testicular cell lines significantly compared to their

corresponding control cells. The viabilities of Ntera-2 cells incubated with 0.25 mg/L;0.5 mg/L;0.75

mg/L and 1 mg/L GA were 65,65%,65,66%,61,13%, and 62,48% respectively. The viabilities of NCCIT

cells incubated with 0.25 mg/L; 0.5 mg/L; 0.75 mg/L; and 1 mg/L GA were 75,20%, 66,59%, 63,37%,

and 57,82% respectively.

Discussion We determined the effects of different doses of GA on the viabities of two types of testicular cells. We

will use these doses in our further apoptosis studies in these testicular cells. This dose study in the

testicular cell has shed light to the literature.



P.7.THE EFFECTS OF THE ADVANCED BIOTECHNOLOGY ON COLD CHAIN AND

TEMPERATURE TRACKING

Sule MENE; Ayse Burcu CEHRELI; Damla KURTUNLUOGLU; Cansu AKKOYUN, Aslı Gurler

Mene Health Group; Gosb Teknopark, Kemal Nehrozoglu Caddesi, Üretim 2-3; Ünite 1-6 41480,

Gebze/ Kocaeli, Türkiye

OBJECTIVE: In many countries, one of the common factors limiting effective and reliable access to

products that require cold chain management and temperature monitoring is the presence of gaps in the

cold chain management and accordingly in the logistics systems. This study focuses on the improvement

of management of the performance of cold chain and address these shortcomings, as well as optimizing

the use of medical products, minimizing waste and achieving some of the essential requirements for

reaching and sustaining these gains.

METHODS: Compilation, interpretation

RESULTS: Existing statistics show that by 2016, 38% of the products developed for the protection and

maintenance of human health require cold chain management and this ratio will reach 80% by 2020.

Today, 75% of the biotechnological products, 15% of the small molecules, all vaccines, biological

specimens and some diagnostic kits are in the scope of the cold chain. Expecting that the 50% of the

new approved drugs will be biopharmaceuticals in the near future, maintaining the efficacy and safety

would be crucial.

DISCUSSIONS: Global and local regulations and statistics regarding cold chain management and

temperature monitoring systems were examined and requirements are evaluated prospectively. With

advanced biotechnology, it is necessary to decrease the risk of contamination, to increase the traceability

and reliability of the product and to maintain its effectiveness between the manufacturing of these

products until reaching the end user. In order to be ready to manage this process properly and to build

proper temperature controlled cold chain transportation and storage systems, global and country specific

legislation should be improved. The process should be controlled and tracked closely by the responsible

parties.

https://www.seslisozluk.net/interpretation-nedir-ne-demek/



P.8.Investigation of Multilocus Sequence Types of Acinetobacter baumannii Isolates and

Comparing the Results with Single Locus Sequence Analysis

Ayşe Nur SARI1,2, Zeynep GULAY2

1 SANKO Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji AD

2 Dokuz Eylül Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji AD

The aim of the study is to determine the sequence types and also clonal complexes of A.baumannii

isolates which were isolated during the years 2000-2011 by using Multi Locus Sequence Typing

(MLST).Thus, it was intended to investigate of relationship between international clones and study

isolates.

PFGE patterns of eight isolates included in the study are known through the MSc thesis which was

accomplished before (isolates from the years between 2000-2011. Optimising of PCR reactions for

seven housekeeping gene locus were completed to determine the STs via MLST method. Sequence

analysis of these locus have been actualised by the service procurument. After the analysis of the

sequence results by the web based tools, allel types of the housekeeping gene locus were assigned.

According to the study findins, all of seven housekeeping gene locus could be determined for eight

isolates and ST types for them as follows; ST133 (isolates of A,A1 and C PFGE patterns), ST103

(isolates of D3 PFGE patterns), ST20 (isolates of B PFGE patterns), ST223 (isolates of D PFGE

patterns), ST55 (isolates of D3 PFGE patterns) and ST365 (isolates of L PFGE patterns).

For the 8 isolates in which sequence types were detected, whole blaOXA-51-like gene locus were obtained

with PCR and 975 bp fragments weresequenced. In the isolates with ST 133 and presented with the

pulso typeA, A1 and C, OXA-51 andin ST20 isolate (with pulso type B),OXA-68 were found. For other

4 isolates (ST103, ST223, ST55 and ST 365), blaOXA-51-like gene locus was found as OXA-66.

Key Words:Acinetobacter baumannii, MLST, OXA-type carbapenemases, sequence type, population

analysis



P.9.Drug target identification through context-specific protein interaction networks with

minimum uncertainty

Aysegul CALISKAN1,2, Dilara AYYILDIZ,3, Esra GOV1,4 and Kazim Yalcin ARGA1,*

1Department of Bioengineering, Marmara University, Istanbul, Turkey,

2Department of Nutrition and Dietetics, Istinye University, Istanbul, Turkey, 3Department of Biomedical Sciences and Biotechnology, University of Udine, Italy,

4Department of Bioengineering, Adana Science and Technology University, Adana, Turkey.

Protein-protein interactions (PPIs) play crucial roles in many cellular processes as well as disease

progression. These interactions are often context-dependent and the reconstruction of context-specific

protein-protein interaction networks in a target condition, such as disease state or drug response, often

requires an extensive view of the potential interactions between a subset of proteins. Current

reconstruction strategies using gene expression data are not considered as good enough because of their

limitations. We report here a novel approach to reconstruct protein-protein interaction networks around

a pre-determined set of proteins based on entropy minimization. Using a comprehensive transcriptome

data in ovarian cancer as case study, we compared the performance of the novel methodology with the

present reconstruction algorithms through employing topological parameters (connectivity, number of

connected components, clustering coefficient), statistical parameters (sensitivity, specificity, precision

and accuracy), and gene set enrichment analyses (disease ontologies). Entropy-based network

reconstruction method outperformed in all perspectives compared to existing strategies. The use of the

proposed reconstructing strategy in further systems biology research might increase estimated efficiency

in the detection of disease-related subnetworks, identification of functional modules and protein

complexes, discovery of novel biomarkers, therapeutic drugs and their targets, and studying disease

mechanisms and comorbidities.



P.10.Metal Based Drug Design Studies Of Citarabine Used In Acute Myelocytic Leukemia Cancer

Treatment

Ozge Eren and Aysegul Golcu*

Department of Chemistry, Kahramanmaraş Sütçü İmam University, 46100 Kahramanmaraş, Turkey.

[email protected]

The new generation drug candidate molecule [Pd(Cyt)2)Cl2]2H2O (Figure) was obtained from

the reaction of palladium(II) salt with the anticancer drug Citarabine (Cyt). This compound has been

characterized by spectroscopic and analytical techniques. Thermal behavior of the compound were also

investigated. The electrochemical properties of the compound has been investigated by cyclic

voltammetry (CV) using glassy carbon electrode. The biological activity of the [Pd(Cyt)2)Cl2]2H2O has

been evaluated by examining their ability to bind to fish sperm double strand DNA (FSdsDNA) with

UV spectroscopy. UV studies of the interaction of the Cyt and [Pd(Cyt)2)Cl2]2H2O with FSdsDNA have

shown that these compounds can bind to FSdsDNA. The binding constants of the compounds with

FSdsDNA have also been calculated. The effect of proliferation Cyt and [Pd(Cyt)2)Cl2]2H2O was

examined on the HeLa cells using real-time cell analyzer with three different concentrations [1].

Figure. The proposed chemical structure of [Pd(Cyt)2)Cl2]2H2O

References

1- A. Golcu, H. Muslu, D. Kılıçaslan, M. Cesme, O. Eren, F. Atas¸ I. Demirtas, Journal of

Molecular Structure, 1119, 2016, 96–109.



P.11.New Metal Based Drug Designs

Aysegul Golcu* and Ozge Eren

Department of Chemistry, Kahramanmaraş Sütçü İmam University, 46100 Kahramanmaraş, Turkey.

[email protected]

Cis-platinum ([Pt(NH3)2Cl2]), developed by Rosenberg in 1969 [1] and used for cancer treatment

has led many research group to synthesize metal-based drugs since those years. In this field, mostly Pt

(II), Zn (II), Ru (III), Cu (II), Mn (III) and (I / IV) are used as the metal ion. In all of those studies, the

drug molecules act as ligands and form mostly monodentate or bidentate molecular strucrures by

offering pair of electrons to metal atoms. The studies of this topic over the last forty years have been

gathered under the roof of “Medicinal Chemistry”. The main objective of Medicinal Chemistry is to

identify new anticancer agents and put them into clinical practice procedures [1]. In addition, one of the

most important reasons that metal-based drugs are used in cancer treatment, is that metal atoms have

variable oxidation step and the ability to be bound to nucleic acid molecules through different

mechanisms.

In this poster, we have been informed about metal-based drug design studies synthesized by our

group and tested on some cancer cell lines like Hela cervical cell culture (Figure).

Figure. Hela cervical cell culture

References

2- S.J. Lippard, Metals in Medicine, Department of Chemistry, Massachusetts Institute of

Technology, pp. 505–585.



P.12.AN INVESTIGATION OF THE RELATIONSHIP BETWEEN MATRIPTASE-2

(TMPRSS6) GENE EXPRESSION, TMPRSS6 GENETIC VARIANTS AND CLINICAL

FINDINGS IN BREAST CANCER

Meltem METE1, Didem CAN TRABULUS2, Canan KELTEN TALU3, Mehmet GÜVEN1, Bahadır

BATAR1

1 Istanbul University, Cerrahpasa Medical Faculty, Medical Biology, Istanbul

2 Istanbul Education and Research Hospital, General Surgery, Istanbul

3 Istanbul Education and Research Hospital, Pathology, Istanbul

Background: TMPRSS6 (transmembrane serine protease 6) is a member of the TTSP (Transmembran

serine proteases) family may play a role in breast cancer susceptibility. TMPRSS6 gene encodes

matriptase 2 that plays an important role in iron hemostasis as hepcidin regulator. We aimed to

investigate the expression levels of TMPRSS6 in normal and tumorous tissues of breast cancer patients,

the relationship of these levels with the pathological findings of patients and the relation of spesific

TMPRSS6 polymorphisms with the hematologic parameters of patients. Material and methods: To

define TMPRSS6 gene expression, normal tissues adjacent to tumor and tumor of 47 patients, who were

diagnosed with breast cancer and underwent surgery, were used. The TMPRSS6 polymorphism study

was performed with 181 breast cancer patients and 90 healthy individuals. Gene expression analysis was

performed by qRT-PCR. Genotyping of TMPRSS6 polymorphisms was performed by RT-PCR.

Results: In our study, TMPRSS6 gene expression levels in tumor tissues were found to be 1,88-fold

higher than gene expression levels in control tissues. When we examined the relationship between

TMPRSS6 gene expression levels and pathological data only a statistically significant relationship was

found between ER and HER2 status (respectively p=0.02, p=0.002). The genotype distributions of

TMPRSS6 gene polymorphisms were examined in relation to hematological findings. Only a significant

correlation was found between rs733655 polymorphism and MCHC parameter in the patient group. In

the rs733655 polymorphism heterozygous and variant homozygotes have higher MCHC values

(respectively p=0.022, p=0.037). In the control group, rs5756506 polymorphism decreased the levels of

variant alleles Hgb (p = 0.017) and Hct (p = 0.03). Conclusions: According to our findings, increased

TMPRSS6 expression in cancerous tissues revealed that matriptase-2 may be effective in the cancer

process and this TMPRSS6 gene polymorphisms may affect the disease process by affecting the blood

parameters of patients.

This work was supported by Scientific Research Project Coordination Unit of Istanbul University.

Project number: 49541

Key words : TMPRSS6, Matriptase-2, Breast cancer, Polymorphisms, Gene expression



P.13.INVESTIGATION OF POLYMORPHISM IN DNA REPAIR ENZYME INTERACTIONS

WITH DNA DAMAGE DETECTION METHODS

Merve BOSTANCI, Mehmet GÜVEN, Bahadır BATAR

Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul

Background: It is known that in the presence of DNA damage, XRCC1 and PARP1 proteins interact

with each other and are involved in the DNA repair protein complex to initiate the base excision repair

(BER). In this study, we aimed to investigate the interactions of XRCC1 and PARP1 proteins according

to the distribution of genotype frequencies of Arg399Gln polymorphism in XRCC1 gene. Materials

and methods: The study consisted of 25 healthy individuals with Arg/Arg, Arg/Gln, and Gln/Gln

variants of XRCC1 Arg399Gln polymorphism. At first, cell cultures were performed from the

lymphocyte samples of these individuals, followed by protein isolation in cells in which DNA damage

was generated by ionizing radiation. Co-immunoprecipitation (Co-IP) study was performed to detect the

interaction of XRCC1-PARP1 proteins and PARP1 levels were determined by ELISA. Results: Our

results showed that XRCC1-PARP1 interaction in the Gln/Gln genotype was markedly higher than the

other genotypes, however, this difference was not statistically significant (P=0.14) Conclusions:

According to our findings, we observed that the interaction of XRCC1 and PARP1 proteins in

individuals with the XRCC1 399 homozygous mutant Gln / Gln genotype was higher than the other

genotype distributions. However, these findings need to be verified by larger study groups.

This work was supported by Scientific Research Projects Coordination Unit of Istanbul University.

Project number: 45060

Key words: XRCC1, PARP1, Ionizing radiation, Polymorphism, DNA repair



P.14.EFFECTS OF PDGF-BB DELIVERY FROM COLLAGEN SUTURES ON THE HEALING

LACERATED CHICKEN FLEXOR DIGITORUM PROFUNDUS MUSCLE

Mousa YOUNESI1, Derrick M. KNAPIK2, Jameson CUMSKY2, Baris Ozgur DONMEZ1, 3, Ping HE1

, Anowarul ISLAM1, Greg LEARN2, Michael BOHL1, Robert J. GILLESPIE3, Ozan AKKUS1,2,4

1Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland,

OH 44106, United States 2Department of Orthopedics, University Hospitals Case Medical Center, Case Western Reserve

University, Cleveland, OH 44106, United States 3Department of Nutrition and Dietetics, School of Health, Akdeniz University, 07070 Antalya, Turkey

4Departmentof Biomedical Engineering, Case Western Reserve University,

Cleveland, OH 44106, United States

AIM

Recent investigations have explored to improve the healing process by growth factor delivery from the

sutures. This study explores the performance of a novel electrochemically aligned collagen suture in a

flexor tendon repair model with and without platelet derived growth factor following complete tendon

laceration in vivo.

MATERIAL AND METHODS

Complete laceration of the flexor digitorum profundus muscle in the third digit of the foot was

performed in 36 skeletally mature White Leghorn chickens. Animals were randomly divided into three

groups: control specimens treated with standard nylon suture (n=12), specimens repaired with

(electrochemically aligned collagen sutures) ELAS suture without (platelet-derived growth factor-BB)

PDGF-BB (n=12) and specimens repaired with collagen suture with affinity bound PDGF-BB (n=12).

Specimens were harvested at either 4 weeks or 12 weeks following tendon repair and biomechanical

testing was performed.

RESULT

Biomechanical testing showed no significant differences between failure load and stiffness values of all

groups at 4 weeks. However, the group treated with PDGF-BB bound suture had significantly higher

stiffness and failure load (p<0.05) at 12-weeks relative to both collagen alone and nylon suture.

Similarly, ultimate tensile strength and Young’s modulus values showed no significant differences

between of all groups at 4 weeks.

DISCUSSION

The ability of ELAS to deliver PDGF-BB to the lacerated area of tendon presents investigators with a

functional bioinductive platform to improve repair outcomes following flexor tendon repair.

Electrochemically aligned collagen suture is a viable vehicle for growth factor delivery, improving

flexor tendon healing grossly and biomechanically following laceration.



P.15.SYNTHESIS AND CHARACTERIZATION OF SALEN BASED POLYURETHANES FOR

BIOSENSOR APPLICATIONS

Birgül Kösteka, Öznur Güngöra, Süleyman Köytepea, Turgay Seçkina aDepartment of Chemistry,

İnönü University, 44280-Malatya, Turkey

e-mail: [email protected]

Salen based ligands and their metal complexes have received considerable attention because of their

various structural properties and medical applications. Especially, salen type molecules have also found

application in antibacterial, antifungal, anthelmintic and catalytically and biological activities [1-3].

Thus, in this work, we aimed to prepare and investigate the biomedical application of salen ligand based

polyurethanes as bioabsorbent, drug delivery system and biosensor membranes. Firstly, salen based

monomers were synthesized from ethylenediamine and vanillin (4-hydroxy-3-methoxybenzaldehyde)

having dihydroxyl functionality. Then, prepared monomer was reacted with different diisocyanates for

produce polyurethane synthesis. After the polymerization, salen ligant based active polyurethanes were

obtained. Chemical structure of the polyurethanes was investigated in terms of FTIR, NMR, TGA,

DTA, DSC, and elemental analysis techniques. Obtained salen unit based polymers were also used for

different metal ions determinations in biological fluids. As a result, prepared polyurethanes bearing

salen ligand unit are good candidates for biosensor and metal iyon absorptions application.

References 1. Sharma, V., Arora, E. K., Cardoza, S., Synthesis, antioxidant, antibacterial, and DFT

study on a coumarin based salen-type Schiff base and its copper complex, Chemical Papers, 70(11)

(2016) 1493–1502. 2. Baudry, M., Etienne, S., Bruce, A., Palucki, M., Jacobsen, E., & Malfroy, B.

Salenmanganese complexes are superoxide dismutase-mimics. Biochemical and Biophysical Research

Communications, 192 (1993) 964–968. 3. Belaid, S., Benali-Baďtich, O., Bouet, G., & Landreau, A..

Synthesis, characterization, and biological activities of oxovanadium(IV) and cadmium(II) complexes

with reduced Schiff bases derived from N,N-O-phenylenebis(salicylideneimine). Chemical Papers, 69

(2015) 1350–1360.



P.16.SYNTHESIS OF METAXALONE AND MEPHENOXALONE TYPE NEW COMPOUNDS

Bayhan KARABULUTa, Ahmet METEa, Cumhur KIRILMISb

Inönü University Faculty of Arts and Science, Department of Chemistry, Malatya

Adiyaman University Faculty of Arts and Sciences Department of Chemistry, Adiyaman

Many 3-aryloxy-1,2-propanediols and their derivatives are widely used in organic synthesis as well as

their pharmacological significance [1]. These diols are important starting compounds of β-blockers,

which are biologically active compounds [2]. On the other hand, oxazole derivatives play an important

role due to their analgesic, antiinflammatory, antidepressant, anticancer, antimicrobial, antidiabetic and

antiobesitic properties [3]. Metaxalone and Mephenoxalone, two of the most important compounds of

this group that contain the oxazolidin-2-one skeleton in the structure, have particularly muscle relaxant

and / or anxiety-reducing properties. Metaxolone is commercially available as Skelaxin®, and

Mephenoxalone is marketed as Dorsiflex®, Moderamin®.

In this study, alternative oxazolidin-2-ones were synthesized for the commercial products metaxalone

and mephenoxalone compounds. Firstly, reaction of the substituted phenols with 3-chloro-1,2-

propanediol in basic medium gave the resultant 3-aryloxy-1,2-propanediol. Oxazolidin-2-ones were

synthesized by reacting 3-aryloxy-1,2-propanediol with urea in polyalkyleneglycol. The products were

characterized by FTIR and 1H-NMR techniques.

[1] Braquet, P., Shen T.Y., Touqui L., Vargaftig B.B.1987. Pharmacol Rev. 39(2): 97-145.

[2] Adhikari, S., Bugga, N., Gurjarl, M., Sadalapure, K., Talukdar, A., Chorghadez, M. 1998.

Heterocycles, (48)7, 1471-1476.

[3] Fozooni, S., Hamidian, H., Tikdari, A. M. 2008. Molecules 13, 3246-3252.



P.17. Determination of total phenolic and flavonoid content, and antimicrobial activity of Rhus

Coriaceae water extract

Bircan ÇEKEN TOPTANCI1, İsmet Rezani TOPTANCI2, Göksel KIZIL1, Murat KIZIL1

1Dicle Üniversitesi, Fen Fakültesi, Kimya Bölümü, 21280, Diyarbakır 2Dicle Üniversitesi, Diş Hekimliği Fakültesi, Çocuk Diş Hekimliği ABD, 21280, Diyarbakır

[email protected]

Dental caries is a multi-factorial infectious disease and despite the great developments in oral health,

tooth caries and periodontal disease are among the most important public health problems in many

countries of the world.

Dental plaque is a biofilm or mass of bacteria that grows on surfaces within the mouth and is commonly

known as the primary cause of dental caries and other oral infections.

Herbal medicines are an important part of healthcare throughout the world. Medicinal plants have been

used as traditional treatments for control of infectious diseases such as dental caries and periodontal

disease. One of the most important genus in the Anacardiaceae family is Rhus which includes more than

250 species. Sumac is the common name for a genus Rhus, which are found in temperate and tropical

regions, worldwide. Rhus coriaria L. was obtained from Uludere, Şırnak in November 2015.

The present study was aimed to determine total phenolic and flavonoid contents, and antimicrobial

activity of Rhus Coriaceae water extract.

Based on the results of the study Rhus coriaceae L. water extract have potential to be used for the oral

hygiene.



P.18.Myeloperoxidase and Total Thiol: The Potential Roles and Their Alteration in End Stage

Renal Disease

Burak ŞENTÜRK, Burcu BABA, Kürşat ÖNEÇ, Musa BALİ, Aysun HACIŞEVKİ

Gazi University, Faculty of Pharmacy, Department of Biochemistry, Faculty of Medicine, Department

of Nephrology, Ankara, TURKEY

Chronic kidney disease (CKD) is a worldwide health problem that may lead to end-stage renal disease

(ESRD). The prevalence of ESRD patients receiving dialysis has been increasing around the world.

Oxidative stress represents one of the underlying mechanisms in the pathogenesis and progression of

CKD, due to both increased production of reactive oxygen species and downregulation of antioxidants.

Myeloperoxidase (MPO) plays an important role in the defense of the organism by catalyzing the

production of oxidants. However, it is a reactive species-generating enzyme that promotes protein

oxidation. Oxidation of plasma thiol groups is quantitatively the major manifestation of protein

oxidation. Abnormalities of the thiol redox balance stand out as a prominent expression of oxidative

stress in CKD. The aim of the study to evaluate MPO and total thiol levels in patients receiving

peritoneal dialysis (PD). The study was performed on 30 patients receiving PD and 34 controls. Serum

MPO levels were measured using Elisa kits and total thiol levels were detected by

spectrophotometrically.

We found that the levels of total thiol as an antioxidant were significantly lower than in patients

compared to healthy subjects (p<0.05). Prooxidant enzyme MPO levels were significantly higher in

patients compared to controls (p<0.05). Our results showed that patients receiving PD are subject to

increased oxidative stress and may have decreased antioxidant defenses. Further studies with larger

sample sizes are needed to clarify the levels of these parameters in patients receiving PD and to develop

therapeutic strategies to reduce oxidant stress in this patient population.

Keywords: Myeloperoxidase, total thiol, end stage renal disease



P.19.MOLECULAR DOCKING STUDY OF RHO-KINASE (ROCK) INHIBITORS

Burcu BAYEL SECİNTİa, Gizem TATARa,Tuğba TASKIN TOKb

a Gaziantep University, Institute of Health Sciences, Department of Bioinformatics and Computational

Biology, 27310, Şehitkamil, Gaziantep, Turkey. b Gaziantep University, Faculty of Arts and Sciences, Department of Chemistry, 27310, Şehitkamil,

Gaziantep, Turkey.

E-mail:[email protected]

Rho-associated protein kinase (ROCK) is a serine / threonine protein kinase and the molecular weight of

its approximately 160 kDa. The enzyme Rho-kinase has two isoforms, ROCK1 and ROCK2. The entire

amino acid sequence of the two isoforms contains about 65% homology, while about 92% homology is

found in the kinase domains. Both enzymes are expressed in all cells. It has been reported that ROCK2

is expressed more in brain and heart, ROCK1 in lung, liver, spleen, kidney and testis [1]. In human

ROCK1 gene is located on chromosome 18 (18q11.1) and the ROCK2 gene is located on chromosome 2

(2p24). RhoA is the most studied Rho protein subtype [2]. It is expressed that the RhoA / Rho-kinase

pathway contributes to various diseases such as Alzheimer, diabetes and cancer [3]. This study aims to

determine the mechanism of interaction at the atomic level by using molecular docking studies that the

inhibitors show therapeutic potential against Rho-kinase (ROCK) enzyme. In this regard, it will be

possible to treat diseases in which the RhoA / Rho-kinase pathway acts with defining less toxic effect

and more specific ROCK inhibitors.

References:

1. Feng Y, LoGrasso PV, Defert O, Li R. Rho Kinase (ROCK) Inhibitors and Their Therapeutic

Potential. Journal of Medicinal Chemistry. 2016; 59:2269–2300.

2. Etienne-Manneville S, Hall A. Rho GTPases in cell biology. Nature. 2002;420:629-35

3. Pan P, Shen M, Yu H, Li Y, Li D, Hou T. Advances in the development of Rho-associated

protein kinase (ROCK) inhibitors. Drug Discovery Today. 2013; 18:1323–1333.



P.20. 4D-QSAR STUDY OF SERINE PROTEASE ACTIVITY OF 2-

HYDROXYDYARILAMIDE DERIVATIVES

Burçin TÜRKMENOĞLU, Yahya GÜZEL

Erciyes University, Faculty of Science, Department of Chemistry, Melikgazi, Kayseri

[email protected]

The invasive nature of tumor cells plays an important role in cancer metastasis. Transmembrane serine

protease II (TMPRSS4) has been derived from the literature as a new type of 2-hydroxydarylamide

derivation series that plays a role in the invasion and metastasis of colon cancer cells1. TMPRSS4 has

been evaluated to inhibit serine protease activity and to suppress cancer cell invasion. These derivatives

served as a good inhibitor against TMPRSS4 serine protease activity (pIC50 (μM)). In this study, the

biological activity of the 2-hydroxydiarylamide derivative series was first calculated by the Molecular

Conformer Electron Topological (MCET) method, a ligand-based QSAR approach. Pharmacophore

structure; (Pha), Auxiliary Groups (AG) and Anti-Pharmacophore Shielding (APS) groups2. This

method has made significant progress both in the determination of the activities of the molecules and in

the emergence of their biological interaction models. The interaction between ligand-receptor for drug

design is shown by 4D-QSAR analysis.

Figure 1: Three dimensional structure of reference molecule

Non-parametric regression analysis was used to determine the adjustable constants for the receptor side.

LOO-CV method was applied for 32 compounds in the training set and 9 compounds in the test set,

respectively, and the q2 and r2 statistical results were found satisfactory.

Acknowledgment: This work was supported by Research Project of Erciyes University, FDK-2016-

6547.

References:

1. Sunghyun, K.; Hye-Jin, M.; Min-Seo, K.; Myung-Geun, J.; Semi, K. Bioorg Med Chem

Lett 2013, 2, 1748–1751.

2. Yilmaz, H.; Boz, M, Türkmenoğlu B.; Güzel Y. Tropical Journal of Pharmaceutical Research.

2014, 13, 117-129.




P.21.INVESTIGATION OF NEW PYRROL DERIVATIVES AS ANTITUBERCULOS AGENTS

WITH MCET METHOD

Ertuğrul ASLAN, Burçin TÜRKMENOĞLU, H. Güzin ASLAN, Yahya GÜZEL

Erciyes University, Faculty of Science, Department of Chemistry, Melikgazi, Kayseri

[email protected]

Mycobacterium tuberculosis (MTB) is a tuberculous lung infection. It is a leading infectious disease that

often causes millions of deaths in developing countries. Pyrrol and its derivatives have anti-tumor,

analgesic, anti-tuberculosis and anti-inflammatory effects.1 Recently, pyrrole derivatives have emerged

as potentially useful chemotherapeutic agents for inhibiting MTB activity.2 In this study, the interaction

between 50 ligand-receptor from the literature, inhibiting MTB activity, was examined using the MCET

method.3 Klopman index from the structural electronic descriptors of the atoms in the molecules was

first used in the pyrrole derivatives to reveal the real interaction points between the enzyme and the

substrate and was determined with the best statistical results. The pharmacophore structure consisting of

b-Pha (basic-pharmacophore), AG (activity enhancer) and APS (activity purifying) groups at these

points was investigated. The nonlinear least squares (NLLS) method in MCET is applied with the

Levenberg-Marquardt algorithm. Thus, the inhibitory effect on tuberculosis was elucidated and the

quantitative relationship between their molecular structures was clearly demonstrated in the 4D-QSAR

method. For the prediction of chemical reactivity in drug discovery and the understanding of chemical

systems, the statistical results of the obtained model were found to be sufficient.

References:

1. Duncan,K.; Barry, C.E. Curr Opin Microbiol. 2004, 7, 460–465.

2. Ahmadi, A.; Solati, J.; Hajikhani, R.;Pakzad, S. Arzneimittelforschung. 2011, 61,296–300.

3. Shrinivas, D.J.; Uttam A.M.; Sheshagiri, R.D.; Haresh H.K. Med Chem Res.2014, 23, 1123–

1147.




P.22.DESIGN AND PREPARATION OF CAFFEIC ACID BASED HYDROGELS FOR

BIOMEDICAL APPLICATION

Büşra Aksoya, Burhan Ateşa, Meltem Kuruş,b Ilgın Türkçüoğluc, Süleyman Köytepea

aDepartment of Chemistry, İnönü University, 44280-Malatya, Turkey

bDepartment of Histology and Embryology, Izmir Katip Celebi University, İzmir, Turkey

cDepartment of Obstetrics and Gynecology, İnönü University School of Medicine, Malatya, Turkey

Email: [email protected]

Hydrogels, widely studied for years in medical applications, still arise exciting interest as

polymeric networks. Especially, hydrogels based on natural resources have been mostly preferred to

biocompatibility, flexibility and low cytotoxicity properties. Due to these unique behaviours, hydrogels

are used in many bio-applications [1] such as scaffolds for tissue engineering, contact lenses, bio-

sensors, nano-carriers, drug delivery systems, etc. The present study has aimed to elucidate the

formation of hydrogels in solutions of cyclodextrin and caffeic acid as a natural resources and to explain

the role of caffeic acid on biocompatibility and adhesion properties of prepared hydrogels.

In this work, a novel hydrogels series was prepared cyclodextrin, caffeic acid and different

molecular weight PEG. Different molecular weight of PEG chains were used to its non-toxic and non-

immunogenic properties. cyclodextrin groups were also used to cross-link in the hydrogel structure. The

polymer formulations were characterized by FTIR, DSC, contact angle and SEM studies. As a result, it

has been determined that the prepared caffeic acid based hydrogels can be used as drug releasing

system, scaffold, mechanic barrier or tissue engineering materials.

This work was supported by Scientific and Technological Research Council of Turkey

(TÜBİTAK) with project number 215Z322.

References

[1] Enrica Caló, Vitaliy V. Khutoryanskiy, Biomedical applications of hydrogels: A review of patents

and commercial products, European Polymer Journal, 65, 2015, 252–267



P.23.THE EFFECTS OF THE ADVANCED BIOTECHNOLOGY ON COLD CHAIN AND

TEMPERATURE TRACKING

Sule MENE; Ayse Burcu CEHRELI; Damla KURTUNLUOGLU; Cansu AKKOYUN, Aslı Gurler

Mene Health Group; Gosb Teknopark, Kemal Nehrozoglu Caddesi, Üretim 2-3; Ünite 1-6 41480,

Gebze/ Kocaeli, Türkiye

OBJECTIVE: In many countries, one of the common factors limiting effective and reliable access to

products that require cold chain management and temperature monitoring is the presence of gaps in the

cold chain management and accordingly in the logistics systems. This study focuses on the improvement

of management of the performance of cold chain and address these shortcomings, as well as optimizing

the use of medical products, minimizing waste and achieving some of the essential requirements for

reaching and sustaining these gains.

METHODS: Compilation, interpretation

RESULTS: Existing statistics show that by 2016, 38% of the products developed for the protection and

maintenance of human health require cold chain management and this ratio will reach 80% by 2020.

Today, 75% of the biotechnological products, 15% of the small molecules, all vaccines, biological

specimens and some diagnostic kits are in the scope of the cold chain. Expecting that the 50% of the

new approved drugs will be biopharmaceuticals in the near future, maintaining the efficacy and safety

would be crucial.

DISCUSSIONS: Global and local regulations and statistics regarding cold chain management and

temperature monitoring systems were examined and requirements are evaluated prospectively. With

advanced biotechnology, it is necessary to decrease the risk of contamination, to increase the traceability

and reliability of the product and to maintain its effectiveness between the manufacturing of these

products until reaching the end user. In order to be ready to manage this process properly and to build

proper temperature controlled cold chain transportation and storage systems, global and country specific

legislation should be improved. The process should be controlled and tracked closely by the responsible

parties.

https://www.seslisozluk.net/interpretation-nedir-ne-demek/



P.24.INVESTIGATION OF MTHFR GENE C677T POLYMORPHISM IN CARDIAC

SYNDROME X PATIENTS

Cemre KANDAZ1, Burak ÖNAL2, Deniz ÖZEN1, Bülent DEMİR3, A. Gökhan AKKAN1, Sibel

ÖZYAZGAN1

1- Cerrahpasa Faculty of Medicine Medical Pharmacology Dept.

2- Istinye University Faculty of Medicine Pharmacology and Clinical Pharmacology Dept.

3- Bakirkoy Dr. Sadi Konuk Training and Research Hospital Cardiology Dept.

Background: Definition of Cardiac Syndrome X (CSX) refers to groups of patients with positive

exercise stress test and normal epicardial coronary arteries on coronary angiography accompanied by

chest pain. Although the etiology of CSX is not completely understood, there is a common consensus

that its pathophysiology may be associated with endothelial dysfunction resulting in impaired coronary

flow. Some polymorphisms observed on the MTHFR gene cause inactivation of the MTHFR enzyme,

leading to hyperhomocysteinemia and homocysteinuria, which are prominent risk factors of

cardiovascular and cerebrovascular diseases.We aimed to explain the association of the endothelial

dysfunction, which is thought to play a role in the pathophysiology of CSX, with C677T polymorphism

on MTHFR gene based on genetic basis.

Methods: 176 patients diagnosed with CSX and 196 healthy subjects with similar age and clinical

features were compared in terms of C677T polymorphism of the MTHFR gene. For this purpose,

genomic DNA of each participant was isolated from drawn blood samples. Then, the gene segment in

interest was proliferated with real time PCR method. After both genotypic and allelic distributions of

CSX patients and healthy controls were obtained, the data were analyzed statistically.

Results and Conclusion: There was no significant difference in terms of MTHFR gene C677T

polymorphism between CSX patients and controls (p=0.194). When genotypic distribution was

compared based on gender in both patients and controls, no significant difference was found between

male and female subjects (p>0.05). These suggest that homocysteine metabolism in CSX is not directly

related to the endothelial dysfunction and thus the effect on the microvascular circulation.



P.25.SIMULTANEOUS DETERMINATION OF LEVODOPA AND BENSERAZIDE IN

PHARMACEUTICAL TABLET AND URINE SAMPLES USING MODIFIED SENSORS

BASED ON PYROL/MULTIWALLED CARBON NANOTUBE

Ebru KUYUMCU SAVAN and Gamze ERDOĞDU

Faculty of Pharmacy, İnönü University, Malatya, Turkey Department of Chemistry, Faculty of

Science, İnönü University, Malatya, Turkey

Levodopa, also named L-dopa, is an effective drug for the treatment of Parkinson’s disease.

However, most administered levodopa is converted into dopamine at periphery, which is caused by

aromatic l-amino acid decarboxylase, leaving only a small amount to enter the brain. Therefore, it is

usually associated with a peripheral aromatic-l-amino acid decarboxylase inhibitor, such as benserazide,

in order to increment the proportion that enters the brain [1].

The objective of this work was to carry out an electrochemical investigation and perform the

electroanalytical determination of levodopa (LD) and benserazide (BZ) at glassy carbon electrodes

modified with multi-walled carbon nano tube (MWCNT) and poly(pyrol). Modified sensors were

prepared by MWCNT and electropolymerized pyrol. The investigated method showed good stability,

reproducibility, repeatability and high recovery in pharmaceutical preparation (94.71 % (BZ) and 99.36

% (LD)) and urine (93.79 % (BZ) and 92.05 % (LD)). Limit of detection (LOD) was found to be 47.9

µM for BZ and 10.2 µM for LD. The proposed method is simple, sensitive, easy to apply and

economical for routine analysis, and these new stable and easy-to-prepare modified sensors represent a

useful alternative strategy for the electroanalysis of BZ and LD in pharmaceutical preparation and urine

samples.

This study was financially supported by the Research Fund Unit of Inonu University (Grant no

APYB: 2013-54)

References 1. Ensafi A.A., Arabzadeh A., Karimi-Maleh H. J. Braz. Chem. Soc., 21(8), 1572-

1580, 2010.



P.26.SIMULTANEOUS DETERMINATION OF LEVODOPA AND CARBIDOPA AT

SWCNT AND POLY(3-METHYLTHIOPHENE) MODIFIED SENSORS: ITS APPLICATION

TO A PHARMACEUTICAL DOSAGE FORM

Ebru KUYUMCU SAVAN and Gamze ERDOĞDU

Faculty of Pharmacy, İnönü University, Malatya, Turkey Department of Chemistry, Faculty of

Science, İnönü University, Malatya, Turkey

Parkinson’s disease is a progressive neurological disorder that occurs when the brain fails to

produce enough dopamine. Dopamine cannot be administered directly because it cannot penetrate the

blood–brain barrier. Therefore antiparkinsonian drug levodopa (L-dopa), which can be orally

administered, is used to provide a source of dopamine [1]. However, since the metabolism of Ldopa is

also extracerebral, several side effects of systemic dopamine can arise if L-dopa is administered in high

dosages. In order to achieve better therapeutic effect and lower toxicity, carbidopa is administered in

association with L-dopa in pharmaceutical preparations containing 10–25% of carbidopa [2].

Hence, the development of a method for the simultaneous determination of L-dopa and

carbidopa is very important, since they are frequently found together in pharmaceuticals. For this

purpose, modified sensor designs have been developed to distinguish levodopa and carbidopa in the

presence of ascorbic acid. Sixteen designed sensors were prepared by modifying single-walled carbon

nano tube (SWCNT) with electropolymerized 3-methyl thiophene. Under optimum conditions, the

electrocatalytic oxidation peak currents of levodopa and carbidopa showed linear dynamic range, good

stability and sensitivity with detection limit of 17.0 µM and 9.4 µM, respectively. The proposed method

has been successfully applied for the determination of levodopa and carbidopa in urine samples and

pharmaceutical preparation.

This study was financially supported by the Research Fund Unit of İnönü University (Grant no

APYB: 2013-54)

References 1. Mohammad MA. et al. Electrochimica Acta, 56, 9113-9120, 2011. 2. Quintino

MSM, Yamashita M, Angnes L, Electroanalysis, 18(7), 655-661, 2006.



P.27.FROM POPULATION GENOMICS TO EFFECTIVE THERAPIES: A CASE FOR

BEHCET’S DISEASE

Goncagül ARPAZ, Efe SEZGIN

Laboratory of Nutrigenomics and Epidemiology, Department of Food Engineering, Izmir Institute of

Technology, Urla, Izmir, Turkey

Abstract

Introduction: Comparison of pathway focused genomic varition within and between world populations

and interpreting results with respect to disease distribution among the examined populations is an

effective way of identifying genetic basis of complex diseases and development of targeted therapies.

Aim: To identify new biological pathways underlying Behcet’s disease (BD) and discover novel

immunological targets for disease modification.

Methods: Populations are grouped according to the prevelance of Behçet’s disease. Population genome

sequence data from 1000 genomes project, Behcet disease related candidate gene and GWAS data from

published studies are analyzed. Ancestral and derived allelic states of disease related genes are

examined. Individual genes, gene groups, and their biological pathways are identified. Current therapy

targets are evaluated based on functional genomic data.

Results: Antigen processing and presentation, cytokine and chemokine signaling, Toll-receptor

signaling, cellular defense response, and proteolysis pathway genes were the most influential on BD.

BD associated variants were mostly derived alleles (66%) with highest frequencies in high BD prevalent

populations suggesting historical immune based selection in these populations. There are still not well

characterized BD associated genes, such as the PSORS1C1 and POU5F1, showing large genetic

differentiation between populations with high BD incidence and others. These genes and related

pathways may indicate potential therapy targets.

Discussion: Population genomic analysis and statistical modelling of genomic data from different

populations within a disease pathogenesis framework can identify novel biological pathways underlying

complex diseases and hint towards more targeted therapies.

Key Words: Population genomics, Behcet disease, Genetic epidemiology, immunologic pathway



P.28.THE EFFECT OF MODAFINIL ON PENICILLIN-INDUCED EPILEPTIFORM

ACTIVITY

Hatice AYGÜN1, Elif ŞEN2, Mustafa AYYILDIZ2, Erdal AĞAR2

1Gaziosmanpaşa University/Faculty of Medicine/ Physiology Department/ Tokat/Turkey

2Ondokuz Mayıs University /Faculty of Medicine/ Physiology Department/ Samsun/Turkey

Aim: Epilepsy patients are also affected by the side effects of anti-epileptic medications and fatigue, in

addition to epileptic seizures they experience. Although modafinil is known as a wake-promoter drug, it

shows anticonvulsant effect on both pentylenetetrazole and electroshock induced seizures. However,

effect of modafinil on acute epileptic seizures is unknown. Therefore, the effect of modafinil on

penicillin-induced epileptiform activity was investigated in rat.

Method: In this study, 35 male rat weighing 180-240 g were used. Under urethane anesthesia, tripolar

electrodes were placed for electrocorticography (ECoG) recording. Modafinil, at doses of 22.5 mg /kg,

45 mg /kg, 90 mg /kg, 180 mg /kg, was administered intraperitoneally, 30 minutes after intracortical

penicillin (500 IU) injection.

Results: Modafinil, at doses of 22,5 mg/kg, 45 mg/kg, decreased the mean frequency of epileptiform

activity 30 minutes and 20 minutes after modafinil injection, respectively whereas modafinil, at doses of

90 mg/kg, 180 mg/kg, decreased the mean frequency of epileptiform activity 50 minutes after modafinil

injection compared to control group (p <0.05) without changing amplitude in all groups (p> 0.05).

Discussion: Mechanisms of modafinil effect are not clear but it is known that modafinil effects GABA,

glutamate noradrenalin, dopamine and serotonin levels in various regions of the brain. However, the

underlying mechanism of modafinil’s anticonvulsant effect on epilepsy need to be enlightened by

advanced analysis methods. Modafinil has the potential to become a new symptomatic treatment agent

as an anticonvulsant and in terms of improving the quality of life epileptic patients.



P.29.INVESTIGATION OF ΑLPHA-LIPOIC ACID SELECTIVE PROPERTIES OF

CELLULOSE-BASED POLYURETHANES IN ELECTROCHEMICAL SENSOR

APPLICATION

Ensar EREL1, Merve Gökşin KARAASLAN1, Süleyman KÖYTEPE1, Burhan ATEŞ1, İsmet

YILMAZ1

Inonu University, 1Department of Chemistry, Malatya, TURKEY

email:[email protected]

The assaying of Alpha-lipoic acid concentration in biological fluids may be an attractive tool for people

health assessment if is considered the correlation existing between LA amount in biologically fluids and

chronic diseases occurrence. In this study, polymeric membranes and electrochemical amperometric

techniques have been successfully used to elimination of the interferant species for determination of α-

lipoic acid. Firstly, a cellulose-containing polyurethane film as α-lipoic acid selective film was

synthesized and characterized. The following reactants were used in the syntheses of polyurethanes:

hexamethylene diisocyanate, cellulose and polyethylene glycol (PEG200). The ratios of PEG:cellulose:

95:5, 90:10 and 85:15, respectively. Structural characterizations of polyurethanes were characterized by

FTIR, DSC, DTA and TGA. Furthermore, polyurethane film was formed by dropping of polyurethane

solution on bare Pt electrode to prepared α-lipoic acid selective electrode. The voltametric results

indicate that the polyurethane based electrodes can be used as a sensor for determination of α-lipoic acid

with the good sensitivity, selectivity, storage stability and rapidly (Figure 1).

Figure 1. DPVs of the polyurethane membrane coating electrodes to increasing concentration of α-lipoic

acid in the presence of interferences and calibration curve of the polyurethane membrane coating

electrodes.

[1]L.Packer, E.H.Witt and H.J.Tritchler, Free Radical Bio.and Med., 19-2 (1995) 227-250

[2]M.Marin, C.Lete, B.N.Manolescu and S.Lupu, J.of Electroanalytical Chem., 729 (2014) 128.

[3]W.Siangproh, P.Rattanarat, O.Chailapakul, J.of Chromatography A, 1217 (2010) 7699-7705



P.30.EVALUATION OF BOTULINUM TOXIN TYPE A EFFECTIVENESS in PREVENTING

POSTOPERATIVE INTRAPERİTONEAL ADHESIONS

Mehmet DOKUR1, Erdal UYSAL2

1Necip Fazıl City Hospital, Department of Emergency, Kahramanmaras

2Sanko University Department of General Surgery, Gaziantep

Purpose: Postoperative intraperitoneal adhesions (PIAs) are one of the most important problems

surgeons have to face after laparotomies. In this study, we aimed to evaluate the effectiveness of local

application of Botulinum toxin type A (BoNT-A) in various dosages on the prevention of intra-

abdominal adhesions in rats with experimental intra-abdominal adhesions.

Method: 40 Wistar Albino female rats were randomly separated into 4 groups. The four groups were

determined as follows: Control (Group 1, n=10); Sham (Group 2, n=10); 10 µg/kg low dose BoNT-A

(Group 3, n=10) and 30 µg/kg high dose BoNT-A (Group 4, n=10). Subserosal injuries were created on

the caecum of all rats. Laparotomy was performed on the fifth day. Adhesion scores, histopathological

examination and E-cadherin expression levels were evaluated.

Result: General adhesion scores for Group 1 and Group 2 was determined to be significantly high when

compared to Group 4 (p<0.001). A significant difference was also determined between Group 3 and

Group 4 in terms of general adhesion scores (p<0.05). In pair comparisons, a significant decrease in

high dose BoNT-A Group (Group 4) when compared to Group 1 and Group 2 in terms of

neovascularization, fibroblast density, collagen deposition and inflammatory cell count was determined

(p<0.05).

Conclusion: A significant decrease was observed only in postoperative PIAs in the high dose BoNT-A

Group between all four rat-groups with experimentally created postoperative PIAs. In this study, high

dose BoNT-A is determined to be an effective agent in preventing postoperative PIAs.

Keywords: Botulinum toxin type A, Rat, Peritoneal adhesion, E-cadherin



P.31.Differential expression of CDKN2B-AS1 and CDKN2B in various human tissues and cancer

cell lines

Esra BOZGEYIK1, Yusuf Ziya IGCI1, Ecir Ali CAKMAK1, Kaifee Arman1, Meltem Koruk Özer1,

Zekiye Altan1, Yunus Sahin1, Ahmet Arslan1

1University of Gaziantep, Faculty of Medicine, Department of Medical Biology and Genetics,

Gaziantep, Turkey

As a molecular disease, cancer is a genetic disorder that results from the deregulation of cell

proliferation and survival of damaged cells. In contrast, normal cells have developed multiple

mechanisms to efficiently and coordinately regulate cell division, differentiation, and cell death

processes. Also, it is now clear that these cellular mechanisms are tightly regulated by non-coding RNA

molecules. Natural antisense transcripts (NATs) which are a class of long non-coding RNAs, are

endogenous RNA molecules that contain complementary sequences with other transcripts. In this study,

our aim was to elucidate the differential expression of CDKN2B, and its NAT CDKN2B-AS1 in various

human tissues and cancer/normal cell lines. In this study, 19 different cell lines and 20 different human

tissues (Human Total RNA Master Panel II) were used. Gene expression levels were analyzed by RT-

PCR and qPCR methods. Consequently, while an expression level of CDKN2B is found to be most

prominent in lung, colon and small intestine tissues, it is found to be expressed at lowest in kidney, liver

and fetal brain tissues. Additionally, while expression of CDKN2B was very low in MDA-MB-231,

PANC1, A172 and CAL29 cells, it was high in CRL4010, HGC27 and HeLa cells. Expression of

CDKN2B-AS1 is found to be similar to expression of CDKN2B cells. Interestingly, according to our

findings, it was found that CDKN2B and CDKN2B-AS1 genes, which are embedded NAT, show a

positive correlation with each other. A complete understanding of the functions of these molecules,

which interact with each other, is of great importance.



P.32.CARDIAC FUNCTIONS FINDINGS in

FACIOSCAPULOHUMERAL MUSCULAR DYSTROPHY

1Caglar Emre CAGLIYAN, 2Hakan GELINCIK, 1Aziz Inan CELIK,

1Rabia Eker AKILLI, 3Filiz KOC

1Cukurova University, Medical Faculty, Department of Cardiology

2Cukurova University, Medical Faculty 3Cukurova University, Medical Faculty, Department of Neurology

Introduction: Facioscapulohumeral Muscular Dystrophy (FSHMD) is one of the most common adult

muscular dystrophies. We aim to investigate structural, functional and arrhythmic abnormalities in

patients with FSHD Type-1.

Material and Methods: Patients with a genetically confirmed diagnosis of Type-1 FSHD, who were on

follow-up by CUMF Neurology Department, were included our study. Control group consisted of age

and sex matched healthy individuals, without any family history of neuromuscular disorders. Left

ventricular mass, left ventricular tissue doppler analysis and myocardial performance index (MPI) were

calculated in Type-1 FSHD and healthy controls. A 24-hour ambulatory electrocardiogram (ECG)

analysis was performed. T wave peak to end (Tp-e) interval and Tp-e/QT ratio was calculated.

Findings: A total of 14 patients and 14 healthy controls were included in our study. Five patients were

female in FSHD group, whereas four healthy individuals were female in the control group (p=0,383). In

the study average heart rate was significantly higher (93.6±10.1 min-1 vs 74.3±5.6 min-1; p=0.000) and

standard deviation of N-N intervals was significantly lower (47.43±15.49 vs 69.85±19.18; p=0.001) in

FSHD Group. Tp-e interval was longer (78.6±14.6 msec vs 64.3±16.5 msec; p=0.031) and Tp-e/QT

ratio was larger (22.9±3.2 vs 17.8±5.4; p=0.018) in the FHSD Group.

Conclusion:These findings suggest that autonomic and repolarization abnormalities might be observed

in patients with FSHD in spite of normal structural and functional myocardial function.



P.33.DATABASE OF NEUROMUSCULAR DİSEASES 1Filiz KOÇ, 2Gizem Gül KOÇ

1Cukurova University School of Medicine Department of Neurology

2Cukurova University Department of Industrial Engineering

Introduction: Neuromuscular diseases are diseases of muscles and nerves that move our bodies. Each

health center archives the initial application and follow-up information of these patients in accordance

with their own registration system. In this study, to develop our registration system in our hospital,

patient information is already running through the filing system where patient cards are located) and to

have a reliable database, it is not a very laborious method such as file scanning, in studies to be done (at

the national level and at the national level), but also to obtain information in a shorter time and in a

shorter time by using database and to obtain regular data entry to the data system (biopsy, Etc.), aiming

to establish a database of neuromuscular diseases in order to inform their families.

Material Method: In the scope of the project, the purpose of the database and the fields required in the

database, the required tables and the table in which each field belongs are identified with the unique

values in each record of the relevant field, the additional tables are created for fields containing

duplicate information, designing the database, designing the relationships, improving the design, and

improving the design.

Results: On the basis of this data, there is the patient's name and surname name, birth place and date,

birth place, place of residence, referral to the clinic, first complaint, self-family history, physical

examination, laboratory data and medical treatment that he was taking. After each patient's EMG report,

biopsy and genetic analysis reports are downloaded from the scanner, PDF, drawn family tree,

neuroimaging (BBT / MRI) methods can be uploaded to the database in JPEG format.

Conclusion: One of the most important problems that these projects need to be overcome in the past is

the necessity of knowing the data input element because these software have a great demand for labor,

because accuracy, security and productivity are crucial in the development of the database project.

Currently there are no nationally-developed database software for specific diseases used by hospitals in

our country. The creation of databases, the development of those created will both save more time and

cost to the healthcare providers and the sites.

This project was supported within the scope of BAP project of Cukurova University TSA-2016-5824.



P.34.Determination of Chronic Inflammatory Demyelinating Poliradiculoneuropathy and

Multifocal Motor Neuropathy Patient Affiliation to the Clinical Neurology Clinic

Filiz Koc, Dilek Iscan, Turgay Demir

Cukurova University School of Medicine Department of Neurology

Introduction: In the autoimmune demyelinating polyneuropathy group, there are symmetrical distal

acquired demyelinating, multifocal demyelinating acquired sensory and motor neuropathy, and MMN.

There is no study showing the frequency of MMN in Turkey, whether it is the KIDP. In this project, it is

aimed to retrospectively screen the records of the patients who applied to the CTU neurology clinic and

to determine the number of patients with KIPD and MMN. As a secondary objective, it is intended to

determine the demographic and clinical characteristics of MMIP and MMN patients.

Material and Method: The files of the patients with an anteversion age of 18 years or older who were

referred to the hospital between 01.01.2010 and 31.12.2016 were reviewed. About 25,200 cases for

KDP and MMN. According to EFNS / PNS guidelines, patients with clinical data of KIDP or MMN

were included in the study. Patients' severity of deep tendon reflex, distribution of sensory disturbance,

other neurologic examination findings, presence of diabetes (AKS, HbA1C levels), accompanying

gammopathy, CSF sample analysis results, cerebral and cervical MRI findings were examined.

Findings: 19 cases were diagnosed as KIDP after file screening. Thirteen of the patients were male and

six were female and the average age was 49 ± 8.6. The onset age of the disease was 43.5 ± 7.2. In two

cases of diabetes mellitus, one case of connective tissue disease was present. Twelve patients had

sensory deficits in all extremities, and two patients had sensory deficits in lower extremities. No sensory

deficits were detected in 5 patients. 10 cases had weakness on all extremities, 6 cases in the lower

extremities, 1 case had diplopia, and one case had incontinence. HbA1C values were higher than 6.0 in

three cases when fasting blood sugar values were high in five of the cases. CSF analysis was performed

on all but one of the cases. CSF protein levels were found to be high (over 100mg / dl in one case) in 16

cases but normal in two cases. At least two nerves in EMNG have ≥50% prolongation of normal values

(NUL) at motor distal latency, ≥30% decrease in NAL from lower limit of normal values (NAL), ≥20%

prolongation of FU wave latency Nerve conduction block, abnormal temporal dispersion was detected.

Cerebral and cervical MRI examinations of all patients were normal.

Conclusion: Even if some patients had a CIDP / MMN diagnosis, it was determined that they did not

have enough history, examination and laboratory data to meet and / or recognize CIDP / MMN

diagnosis according to the EFNS / PNS guidebook criterion. This showed the importance of detailed

history, detailed examination and electrodiagnostic examination in these cases.

This project was supported within the scope of BAP project of Çukurova University TSA-2016-5636.



P.35.A novel in silico methodology for drug target identification based on differential interactome

Gizem GULFIDAN1, Beste TURANLI1, 2, Kazim Yalcin ARGA1

1 Department of Bioengineering, Marmara University, 34722 Istanbul, Turkey

2 Department of Bioengineering, Istanbul Medeniyet University, 34730 Istanbul, Turkey

Protein-protein interactions are very important in almost all biological processes. Therefore, they should

be considered as therapeutic targets for drug development process. However, efficient algorithms are

required to predict novel therapeutic targets. Here, we present a novel in silico drug target identification

methodology to determine drug targets in diseases. The methodology integrates gene or protein

expression profiles with protein-protein interactions (PPIs) to determine the differential interactome in

the disease of interest, and employs gene-drug-disease (GDD) associations to predict candidate drugs.

Prostate cancer was chosen as case study. Our algorithm was implemented to the gene expression data

of 252 prostate (52 control and 200 tumor) samples, from The Cancer Genome Atlas (TCGA). Utilizing

high confidence human protein interactome, PPIs representing minimum uncertainty were determined as

the differential interactome in prostate cancer. Consequently, 177 significant PPIs among 180 proteins

were determined. Among these proteins, 9 highly interactive proteins (7 of those were repressed and 2

of those were activated in disease condition) were detected as drug targets for repositioning procedure.

In the future works, candidate drugs will be predicted through repositioning, and docking and molecular

dynamic simulations will be performed on the interactive behavior of selected proteins under the

absence and presence of the candidate drugs. Then, the effectiveness of the candidate drugs will be

experimentally verified on prostate cancer cell lines.



P.36.Insulin Receptor Substrate (IRS) Proteins as Therapeutic Target in Cancer

Gokhan Gorgisen1, Ismail Musab Gulacar1, Zafer Cetin2

1Yuzuncu Yil University, Faculty of Medicine, Department of Medical Biology,Van, Turkey

2Sanko University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey

The insulin-like growth factor (IGF) pathway mediates cancer cell proliferation, survival, and

metastasis. IRS proteins are the main adaptor molecules that bind to insulin-like growth factor receptor

(IGFR) and induce the activation of PI3K and MAPK pathways results in proliferation and metastasis of

the cell. Recent publications have shown over-expression of IRS1 in hepatocellular, ovarian,

medullablastoma, pancreas, and prostate cancer, moreover, this expression was linked to poor prognosis

and metastasis whereas it was correlated with well prognosis in squamous lung cancer and breast cancer.

In addition to these datas and its involvement in IGFR signaling, binding of IRS1 to certain growth

factor receptors, leptin, integrins, vascular endothelial growth factor receptors, are the master regulators

of the carcinogenesis, shows the pivotal roles of these proteins in cell proliferation, tumorigenesis and

metastasis. In the light of these datas, many scientists suggested that IRS proteins and their downstream

proteins may be used as a therapeutic target in cancer. In this review, we will present the recent

personalized treatment options that target this family members and its downstream elements in cancer.



P.37.A Computational Study of Molecular Recognition Properties of Tripodal-Amides Bearing 6-

crown-16 and 5-crown-15 Units with Organic Cations.

Gülşen Öztürk, Sevil Şeker, Necmettin Pirinççioğlu

Dicle University, Department of Chemistry, Diyarbakır, 21280 TÜRKİYE

Molecular recognition is a well-known phenomenon in nature and this has been well-established by

model studies. This study involves the synthesis of two tripodal receptors bearing 6-crown-16 (1) and 5-

crown-15 units (2) and their recognition behaviours against some organic cations. The work bears

significant data which may provide a basic understanding of cation recognition, which is very important

in signal transcation in cells. The molecular dynamic calculations using Assisted Model Building with

Energy Refinement (Amber) were performed to understand the conformational changes in the

complexes and also to estimate the binding free energies of receptors with cations. It was found that 1:3,

2:3 and 1:1 stoichiometries were prefered for mono-, di- and tri-valent cations, respectively.

R1 (n=2), R (n=3)

L1

L2

L3

L4

Referances

1. Beer PD, Shade M (1997) Solvent dependent anion selectivity exhibited by neutral ferrocenoyl

receptors. Chem Commun 24:2377-2378.



P.38.The Use of Chiral Ferrocene Schiff Base Ligand in Molecular Recognition and Measurement

of T1 and T2 Relaxation Times by NMR Spectroscopy and Calculation of Binding Constants

Gülten Kavak, Veysel Turan, Gülsen Öztürk

1Dicle University, Department of Physics, Diyarbakır, 21280 TURKEY 2Dicle University, Department of Chemistry, Diyarbakır, 21280 TURKEY

The most important of chirality studies which is the main theme of modern chemistry is result of

interaction with other chiral molecules. Chiral molecules can behave very differently in biological

systems due to their different shapes in 3-dimensional space. Although the physical differences between

the two forms of the optically active molecule are small, the orientation of a single functional group

change the properties of the molecule. Chiral ferrocene ligands are distinct classes of stereo selective

synthesis compositions and their development is very fast. Ferrocene carboxaldehyde and the study of

the schiff base derived from their metal complexes have been quite interesting. The importance of

ferrocenes in asymmetric synthesis is that many ferrocene derivatives are quite successful as chiral

catalysts.

In this study, the molecular recognition properties of chiral ferrocene schiff bases ligand synthesized

were investigated by 1H NMR method. Binding constants were calculated from the 1H NMR chemical

shifts versus varying chiral amine salt concentrations at the constant host concentration. The binding

constants of the complexes formed by the enantiomers of R- and S- phenylethylamines of chiral

ferrocene schiff base were calculated as Ka=265.96x10-3

M-1

and Ka= 273.70x10-3

M-1

,

respectively. The both binding constants were evaluated too weak as a compared with the finding of

Ka<105

M-1

in the research of Lee Fielding (2007). It was concluded that ligand had better and highest

enantioselectivity to S- enantiomer compared with R- enantiomer of phenylethylamine. In addition 1H

NMR T1 spin-lattice relaxation times were measured versus varying chiral amine salt concentrations at

the constant host concentration too. This conclusion is also supported by graphs plotted against the

concentrations of S -and R -phenylethylamines of 1 / T1

(1R)-2-(ferrocenylideneamino)-2-ethyl-cyclohexane S- phenylethylamine

Referances

1. Lee Fielding NMR methods for the determination of protein–ligand dissociation constants

Progress in Nuclear Magnetic Resonance Spectroscopy, 51 (2007) 219–242



P.39.THE EFFECT OF HIGH DOSE AGOMELATİNE ON SPIKE WAVE DİSCHARGES IN

WAG/Rij RATS

Hatice AYGÜN1, Elif ŞEN2, Mustafa AYYILDIZ2, Erdal AĞAR2

1.Department of Physiology Faculty of Medicine, University of Gaziosmanpaşa Tokat/Turkey

2.Department of Physiology, Faculty of Medicine, University of Ondokuz Mayıs Samsun/Turkey

Aim: Agomelatine has been shown to be effective in a number of animal models of neurodegenerative

diseases. Genetically epileptic WAG/Rij rats develop spontaneous absence-like seizures after 3 months

of age. In this study, the effect of high dose agomelatine was investigated on absence epileptic activity

in WAG/Rij rats.

Methods: Tripolar electrodes were placed on skull in order to perform ECoG evaluation. Following the

recovery period, elektrocorticography records (ECoGs) were recorded at 09:00 am for 2 hours every

day. Subsequently, normal saline (Group I; 1 ml/i.p) and agomelatine (Group II: 80 mg/kg/i.p) were

administered intraperitoneal (i.p). After injection, ECoGs were recorded for another 2 hours. The total

number, the total duration and the amplitude of the spike-wave discharges (SWDs) were calculated

offline in every ten minutes.

Result: Agomelatine (80 mg/kg/i.p) reduced the total number and the total duration of SWDs, without

changing the amplitude (p<0,05). Saline at 1 ml/i.p did not alter any of these parameters (p>0.05). The

amplitudes of SWDs were not altered in all groups (p>0.05).

Conclusions: Agomelatine is both a melatonin M1 and M2 receptor agonist and 5HT-2C receptor

antagonist, the affinity of agomelatine for the 5-HT2C receptor is in the micromolar range and about

100-fold less than its affinity for melatonin receptors. It might be concluded that, agomelatine may

reduce epileptiform activity in WAG/Rij rats through regulating the circulation sleeping by melatonin.

Further studies are needed to find out certain mechanism of these effects.



P.40.Synthesis and Characterization of Novel Chiral Thiosemicarbazides Derived

from D- and L-Phenylalanine Esters and Their Metal Complexes

Hatice Gamze SOGUKOMEROGULLARI1, Eyüp BAŞARAN1, Ayşegül KARAKÜÇÜK-İYİDOĞAN1,

Emine Elçin ORUÇ-EMRE1, Mehmet SÖNMEZ1

1Gaziantep University, Arts and Sciences Faculty, Department of Chemistry, 27310 Gaziantep, Turkey

Thiosemicarbazides are valuable compounds that have different applications in the area of medicine, chemisty

and pharmaceutical chemistry. Especially in recent years, there have been some intensive studies about their

synthesis and biological activities such as anticancer, antibacterial, antifungal, antioxidant and antituberculosis

activity. The increasing studies on thiosemicarbazides and their complexes have encouraged us to study in this

area.[1-3] Moreover, ~56% of the drugs are chiral compounds and over 80% of these chiral drugs are used in their

racemic mixtures in clinical treatment.[4]

In this study, new two chiral thiosemicarbazide ligands and their metal complexes were synthesized. All

compounds were characterized by elemental analysis and UV-Vis, Mass, FT-IR, NMR spectral analysis.

NH

NH

HN

O

SO O

NO2

HN

SNO2

NH

NH

HN

O

SO O

NO2

HN

SNO2

(R) (S)

Figure1. Structure of Ligands

Keywords: Thiosemicarbazide, metal complexes, characterization

References

1. T.A. Yousef, O.K. Alduaij, Sara F. Ahmed, G.M. Abu El-Reash, O.A. El-Gammal; Journal of Molecular

Structure. 2016, 1119, 351-364.

2. T.A. Yousef, G.M. Abu El-Reash, O.A. El-Gammal, B.M. Sharaa; Egyptian Journal of Basic and Applied

Sciences. 2016, 3, 44–60.

3. I. O. Mohamed, H. A.-R. Mohamed; Polyhedron. 2015, 98, 162–179.

4. I.K. Reddy, T.R. Kommuru, A.A. Zaghloul, M.A. Khan; Crit Rev Ther Drug Carrier Syst.

2000,17, 285–325.



P.41.DETERMINATION OF CELL ADHESIVE PROPERTIES OF BORIC ACID

CONTAINING POLYIMIDE FILMS

İmren ÖZCAN1, Sevgi BALCIOĞLU1, Burhan ATEŞ1, Süleyman KÖYTEPE1, Turgay SEÇKİN1

1Inönü University, Faculty of Arts and Sciences, Department of Chemistry, 44280 MALATYA

Polyimides have commercial predominance due to their superior properties such as high thermal

stability, excellent mechanical and electrical properties, high dimensional stability and selective gas

permeability [1]. Nowadays, polyimides were used on aerospace, automotive, military, electronic,

environmental protection and biomedical applications. In the biomedical application, polyimides were

used in encapsulation of biomedical devices due to their biosuitability and biocompatability properties;

some orthopedic implants due to highly mechanic properties, in neural implants due to the good

electrical properties. Devices made of polyimide have elicited only mild foreign body reactions in

several applications in the peripheral and central nervous system showing good surface and structural

biocompatibility [2]. In this study, cell adhesion properties of polyimide films with boric acid at

different ratios were investigated.

Experimentally, the polyimide was synthesized by Schlenk technique. Polyamic acid were

obtained from 1,5-diaminopyridine and pyromellitic dianhydride in NMP solvent, and then boric acid in

different proportions were doped in polyamic acids. Boric acid containing polyimide composites were

prepared from these poliamic acids with thermal imidization methods. Obtained polyimide composites

were characterized FTIR, elemental analysis, SEM and X-ray techniques. Thermal properties of

composites were also determined by TGA and DSC. The biocompatibility of structurally characterized

polyimide films was achieved using the L-929 cell line in the cell culture system.

References

[1] Ghosh,M.K., Mittal, K.L., 1996 “Polyimides, fundamentals and applications” Mercel Dekker, Inc.

[2] Georgiev, A., Dimov, D., Spassova, E., Assa, J., Dineff, P., Danev, G., 2012, “High Performance

Polymers - Polyimides Based – From Chemistry to Applications” InTech



P.42.GENOMIC MEDICINE IN PANCREATIC CANCER

Gokhan Gorgisen1, 2, Ismail Musab Gulacar1, 2

1Yuzuncu Yil University, Faculty of Medicine, Department of Medical Biology, Van, Turkey

2Yuzuncu Yil University Technocity, Genovan Genetic and Biotechnology, Van, Turkey

Pancreatic cancer retains a poor prognosis among the gastrointestinal cancers with a 5 year overall

survival of less than 5%. Many patients with pancreatic cancer are diagnosed at advanced stages because

of the absence of screening and indications. Chemotherapy and radiotherapy are standart treatment

options after surgical resection in pancreatic cancer. Gemcitabine, is a pyrimidine analog, represents the

standart chemotherapy in all stages of pancreatic cancer. However, gemcitabine resistance is developed

in patients due to the heterogeneous nature of the disease. Therefore, determining the molecular

biomarkers have a crucial role for developing targeted therapies options in the treatment of pancreatic

cancers. Currently, many gene mutations and expression changes, are responsible for developing

pancreatic cancers, are determined. In addition to these datas, few SNPs and their effects on treatment

options are also clarified in recent studies. In this review, recent pharmacogenomics and genomics

approaches in pancreatic cancer are presented.



P.43.Tissue specific reporter biomolecules in ovarian cancer: novel biomarkers and candidate

therapeutic targets

Esra Gov1,2, Medi Kori1, Kazım Yalcın Arga1

1 Department of Bioengineering, Marmara University, Istanbul, Turkey

2 Department of Bioengineering, Adana Science and Technology University, Adana, Turkey

Ovarian cancer is a growth of abnormal malignant cells that begins in the ovaries. Tumors are complex

tissues comprising not only malignant cells but also genetically stable stromal cells. Understanding the

molecular mechanisms behind epithelial-stromal crosstalk in ovarian cancer is a great challenge.

In the present study, we performed independent analysis of ten transcriptome data from laser micro-

dissected epithelial, stromal and ovarian tumor tissues. Using a multi-omics data analysis framework,

differential expressed genes (DEGs) were obtained for each dataset and tissue specific reporter

biomolecules (genes, receptors, transcription factors, microRNAs and metabolites) were identified based

on aberrant gene expression profiles through integration of the DEGs with genome-scale biological

networks. Furthermore, disease affected biological and metabolic pathway information was gained from

enrichment analyses. Tissue specific comprehensive networks including mutual DEGs and reporter

molecules were reconstructed and topological analysis were performed.

The comprehensive networks for each tissue may represent tissue specific potential effective biomarkers

and pharmacologically tractable therapeutic targets. It may suggest that common reporter biomolecules

among CEPI, stroma and tumor tissues may be more efficient than tissue specific biomolecules.

Therefore, CDK2, EP300 and SRC as receptor related functions or membrane proteins, ETS1, AR,

GATA2, and FOXP3 as transcription factors, miR-16-5p and miR-124-3p as miRNAs were predicted as

candidate biomarkers and therapeutic targets for further experimental studies. Especially, GATA2 was

proposed as a novel biomarker for ovarian cancer. This study in overall represents tissue specific

targetable reporter biomolecules for diagnostic and therapeutic purposes.



P.44.Yoghurt Production Yeast Obtained From Some Legumes Will Be Produced by

Microbiological Methods, Characterization and İnvestigation of Quality Control

Mesut ÇAY, Eda CİHAN, İbrahim Halil KILIÇ, Mehmet ÖZASLAN, Fatih YAYLA

Gaziantep Universty, Science and Literature Faculty, Moleculer Biology, Gaziantep


1. Purpose of Project

In this study, belonging to fabaceae family is intended to be obtained from 4 types of yoghurt. For this

purpose yeast is primarily supplied as ready to be performed on the stage of obtaining pasteurized

milk,milk fermented with yeast that is obtained following the different types of yogurt bacteria in yogurt

with herbs and can be found in characterization, quality control was placed in the middle of the process

will be introduced. Thus, the microbiological richness of the country will be revealed.

2. Methods and Procedures

2.1 The Collection of Plants and Nomenclature : Species of plants which are to be supplied on a

commercial basis at the level of the naming process, Gaziantep University, Faculty of Science and

Literature, Department of Biology, Highland conqueror will be carried out by a research assistant.

2.2 Milk To Be Used ın The Production of Yoghurt: In the production of yogurt, daily shopping

stores which can be obtained from pasteurized milk (UHT) will be used.

2.3 Before Yeast, After Production of Yogurt: In the production of yogurt, fermented milk milk

production in the Turkish food Codex has been determined considering the conditions made ready for

the communication. Manufacturing, Gaziantep University biology department will be made.

2.4 Microbiological Analysis Applied To Yogurt Samples: Dilution preparation Lactobacillus

bulgaricus, Streptococcus thermophilus Bifidobacterium longum Lactobacillus count in the Gasser

2.5 Phenotypic Characterization: Bacterial isolates asmaha (2001), according to API 50 CH using the

system will be described.

2.6 Applied Sensory Analysis of The Yoghurt Samples: In our study, we have recommended that the

consumption of yoghurt sensory analysis in order to test the quality of this analysis will be conducted

and scoring test will apply.

3. Discussion

By evaluating data obtained from the study of yoghurt production from milk rising with different types

of plants belonging to fabaceae family, phenotypic characterization of bacteria isolated from yoghurt

and yoghurt for the quality control of the data will be introduced.



P.45.THE COMPARISON OF THE HEALTH BRAIN LOBE DOSE USING THE DIFFERENT

TREATMENT PLANNING TECHNIQUES IN GLIOBLASTOMA MULTIFORME

TREATMENT

Murat KARABAŞ1, Can DEMİREL2 1Dr. Ersin Arslan Training and Research Hospital, Department of Radiation Oncology, Gaziantep,

[email protected] 2Gaziantep University, Medicine Faculty, Department of Biophysics, Gaziantep, [email protected]

Pupose: In our study, health brain lobe doses were compared using tree-dimensional conformal

radiotherapy (3D-CRT) and intensity-modulated radiation therapy (IMRT) planning techniques, which

were used as Standard techniques in the treatment of glioblastoma multiforme (GBM).

Method: We compared IMRT (Field in Field-FIF and inverse) and 3D-CRT radiation trratment plans for

19 patients with glioblastoma multiforme. Target volumes and organs at risk (OAR) were defined on CT

images. The prescribed dose was 60Gy/30# at 2Gy/# to the PTV60. According to the RTOG protocol,

the plannings were designed, all of the target volumes receive 95% of the defined dose. As a result of

using different planning techniques, the doses of the health brain lobe was checked.

Results: In all 19 patients, our study were compared according to different planning techniques for the

values of V60, V50 and V40 dose volume of health brain lobe without brain tumor. There was no

statistically significant difference in the comparison of 3D-CRT and FIF planning techniques over

health brain lobe (p=0.054). A comparison of 3D-CRT with inverse planning techniques revealed a

41.3% difference (p=0.004) in the V40 percent volume, a 54.2% difference (p=0.001) in the V50

percent volume and a 85.3% difference (p=0.001) in the V60 percent volume of the health brain lobe.

Discussions: In our study, the health brain lobe of IMRT plans was found to be quite low. Simultaneous

chemotherapy with radioterapy in the treatment of malignant brain tumors improves the efficacy of the

treatment but also increases the risk of radiation necrosis. IMRT planning techniques should be used to

reduce the risk of late radiation necrosis.

Conclusions: These results indicate that in the treatment of patients with GBM, inverse planning

protected better critical organs than other techniques and improved target coverage. When FIF planning

and dose volume histograms of the 3D-CRT techniques were compared, it was seen that the FIF

technique performed better tissue protection. Considering the possibilities and workload in the clinic,

the use of intensity-modulated radiotheapy techniques in the radiotherapy of patient with GBM is

recommended.

Keyword: GBM, IMRT, 3D-CRT, Brain tumor



P.46.Assessment of Anticancer Activity of Phlomis pungens on Breast, Lung, Prostate and

Cervical cancer cells

Önder YUMRUTAŞ1, Mustafa PEHLİVAN2, Munevver SOKMEN3,İbrahim BOZGEYİK1, M. Özgür

ÇEVİK4, Ahmet ARSLAN5

1University of Adiyaman, Faculty of Medicine, Department of Medical Biology, Adiyaman, Turkey

2University of Gaziantep, Vocational School of Nurdagiı, Department of Medicinal and Aromatic

Plants, Gaziantep, Turkey

3University of Konya Food and Agriculture, Faculty of Engineering, Konya, Turkey

4University of Adıyaman, Faculty of Medicine, Department of Medical Genetic, Adiyaman, Turkey

5University of Gaziantep, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey

Aim: To determine of natural materials that may have drug potency in anticancer studies, in this study

we aimed to determine the anticancer activity of Phlomis pungens on breast, lung, prostate and cervical

cancer cells.

Method: Firstly, P.pungens were sequentially extracted with hexane, dichloromethane and methanol by

using Soxhlet extractor apparatus. MTT assay was used to determine the cytotoxic effects of extracts on

cancer cells. Apoptosis induction was determined in FACS apparatus with Annexin V and propodium

iodide. Then, DNA was isolated from cancer cells to which the extracts were applied and it performed

on electrophoresis to determine fragmentation. Also, cellular antioxidant activity (CAA) of extracts was

measured on cancer cells. Lastly, phenolic contents were screened by HPLC.

Finding: Only DCM and MeOH extracts from these extracts exhibited cytotoxic effect. DCM extract

induced apoptosis in cervical cancer cells, whereas MeOH extract did not induced. It was observed that

the DNA was disintegrated as a swab. The strongest cellular antioxidant activity was determined in

MeOH extract. In addition, protocatechuic acid, catechin, chlorogenic acid, caffeic acid, o-coumaric

acid and quercetin in methanol extract were determined.

Conclusion: It has been determined that the semi-polar DCM extract of P. pungens exhibits anticancer

activity through apoptosis induction in cervical cancer cells.

Key words: Cytotoxicity, Apoptosis, DNA fragmentation, cellular antioxidant activity, Phytochemicals.

Acknowledgement: This study was supported by the project TUBITAK 112S572.



P.47.DETERMINATION OF CATECHIN USING A POLYIMIDE BASED AMPEROMETRIC

BIOSENSOR

Süleyman KÖYTEPE1, Nurcan AYHAN1, Serap TITRETIR1, Aziz PAŞAHAN1

1İnönü University, Faculty of Arts and Science, Chemistry Department, 44280, Malatya, Turkey


Catechin is one of the most significant polyphenol, which in a anticancer agent. Catechin is abundantly

contained in fruits, vegetables, tea, chocolate and wine[1]. There are many techniques having been

reported for catechin determination, including liquid chromatography, spectrophotometry, gas

chromatography, and electrochemical method. Among mentioned techniques, electrochemical method

for the determination of catechin are preferred over others due to its very fast response time, low

detection limit, low cost and easy of fabrication[2]. As polymeric materials, polyimides (PI) exhibit

various important properties, such as excellent physical and chemical resistance, high-temperature

stability, adhesive properties, high mechanical strength, superior mechanical, and electrical properties

[3]. Hence, polyimide films are widely used in the field of sensor and permselective membranes. In this

research, a new series of polyimide has been synthesized and characterized by means of FT-IR, DTA,

DSC, TGA. TGA results showed that these polyimides were thermally stable. Therefore, the synthesized

polyimides were used as a membrane for the voltametric determination of catechin in the presence of

interference compounds. These polyimides were formed by casting the film on the bare platinum

electrode surface. The selectivity behaviour of prepared polyimides films was investigated by

differential pulse voltammetry (DPV). Using this polyimide, it was possible to obtain a good catechin

selective electrode.

References

[1] D. A. El-Hady, Anal Chim Act. 593 (2007) 178–187.

[2] W. Xiao-Gang, L. Jing, F. Ya-Juan, Microchim. Acta. 169 (2010) 173–179.

[3] W. Sheng, Q. Chen, P. Yang, C. Chen, High Performa




P.48.Genome-Wide Analysis of SIP1 Binding Sites by ChIP-Sequencing in Hepatocellular

Carcinoma Cells with High Endogenous SIP1 Expression: Unraveling New SIP1 Targets from

Multiple Pathways.

Pelin Balcik-Ercin1, Metin Cetin1, Irem Yalim-Camci1, Gorkem Odabas1, A. Emre Sayan2 and Tamer

Yagci1

1: Gebze Technical University, Department of Molecular Biology and Genetics, Laboratory of

Molecular Oncology, C2 Building, 41400, Cayirova, Kocaeli, Turkey.

2: Cancer Sciences, University of Southampton, Somers Building, Tremona Road, Southampton, SO16

6YD, UK.

ABSTRACT

Smad-interacting protein 1 (SIP1) is among transcriptional repressors that regulate epithelial-to-

mesenchymal transition. Recent studies indicated that SIP1 not only downregulates E-Cadherin

expression but also regulates multiple pathways by modulating the expression of CCND1, hTERT,

CLDN4, ALPL and miR-200 family by binding to bipartite E-box motifs in gene regulatory regions.

Given the high frequency of E-boxes on the genome and multiplicity of pathophysiological processes

regulated by SIP1, we hypothesized that aforementioned genes represent only a small fraction of SIP1

targets. Hence, we designed a ChIP sequencing study in high-SIP1 expressing SNU398 hepatocellular

carcinoma cells by using homemade anti-SIP1 monoclonal antibody, clone 6E5. 509 genes were

annotated and intervals related to these genes were found to include SIP1 binding motif “CACCTG”

according to MEME/TOMTOM motif analysis software. ChIP validation of SIP1 targets in SNU398

cells showed significant enrichment for miR200, CDH4, ADARB2 and LPCAT1 genes. We detected

differential expression of BOK, MAP7 and GALNT3 genes between SIP1-silenced and control clones

of SNU398 cells. In addition, validation of SIP1 binding to these differentially expressed genes in ChIP

assays strongly suggested that SIP1 might regulate their expression. Our results that SIP1 is involved in

the regulation of genes with functions as diverse as in apoptosis, glycosylation and microtubule

dynamics add new levels of complexity to the understanding of the role of SIP1 in EMT and cancer

metastasis.



P.49.ARE THE FOODS USED FOR THE WEAKENİNG RELİABLE?

Saadet Ozen

SANKO University, Faculty of Health Sciences, Department of Nutrition and Dietetics

Nowadays you have heard that many foods are referred to as "miracle food", "weakening food". Many

people who want to weaken each summer, especially when they approach each summer, recommend

some foods to each other, and each year on dieting glasses they "come" or "find" for another food. Are

these foods really weighty or reliable?

Let's start with the green tea, which is very popular among beverages. There are many studies in the

literature on green tea and green tea extract. Most of them are concentrated on epigallocatechin gallate

(EGCG) catechins and caffeine from in their content. It has been reported that the mechanism of action

increases energy expenditure, reduces lipogenesis and fat absorption (1,2,3). However, the compilation

of literature results and the results of a meta-analytic study have shown that this effect is less and

statistically insignificant (4,5).

When the miracle drink continues, it is necessary to give the second order to the green coffee bean. The

possible mechanism of action is to prevent fat accumulation and regulate glucose metabolism. There are

few studies that are not methodologically sufficient to show that moderate effects on body weight are

present, but there are not as many studies supporting this. We also report headache and urinary tract

infections among the reported side effects (6).

We can not ignore blueberries in miraculous foods that come to mind in food. The possible mechanism

of action of these nutrients is to change lipid metabolism. When we look at the literature, we see that the

effect emerges in combination with other components. No side effects were found. But this is not as

much work as we can say that there is an effect on weight loss (7).

References

1. Bérubé-Parent S., Pelletier C., Doré J., Tremblay A., Effects of encapsulated green tea and Guarana extracts

containing a mixture of epigallocatechin-3-gallate and caffeine on 24 h energy expenditure and fat oxidation in men.

Br J Nutr. 2005 Sep;94(3):432-6.

2. Dulloo A.G., Duret C., Rohrer D., Girardier L., Mensi N., Fathi M., Chantre P., Vandermander J. Efficacy of a

green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation

in humans. Am J Clin Nutr 1999;70:1040–5.

3. Hodgson JM, Puddey IB, Burke V, Croft KD. Is reversal of endothelial dysfunction by tea related to flavonoid

metabolism? Br J Nutr 2006;95:14–7.

4. Jurgens T.M., Whelan A.M., Killian L., Doucette S., Kirk S., Foy E. Green tea for weight loss and weight

maintenance in overweight or obese adults. Cochrane Database Syst Rev. 2012 Dec 12;12:CD008650

5. Phung O.J., Baker W.L., Matthews L.J., Lanosa M., Thorne A., Coleman C.I. Effect of green tea catechins with or

without caffeine on anthropometric measures: a systematic review and meta-analysis. Am J Clin Nutr. 2010

Jan;91(1):73-81

6. Vinson J.A., Burnham B.R., Nagendran M.V., Randomized, double-blind, placebo-controlled, linear dose, crossover

study to evaluate the efficacy and safety of a green coffee bean extract in overweight subjects, Diabetes Metab

Syndr Obes. 2014; 7: 467

7. H.M. Lehtonen, J.P. Suomela, R. Tahvonen, B. Yang, M. Venojärvi, J. Viikari and H. Kallio, Different berries and

berry fractions have various but slightly positive effects on the associated variables of metabolic diseases on

overweight and obese women, European Journal of Clinical Nutrition (2011) 65, 394–401



P.50. HUNT FOR NOVEL GENES ASSOCIATED WITH CILIA

BIOGENESIS/CILIOPATHY

Sebiha CEVIK1, Enes CİCEK1, İsmail GUMUSTOP1, Oktay I. KAPLAN2

1Department of Life and Natural Science, Abdullah Gul University, Kayseri, Turkey

2Faculty of Medicine, Istanbul Medeniyet University, Istanbul, Turkey

Cilia are evolutionarily conserved hair-like structures that project from the surfaces of most vertebrate

cells. They have been found to play a number of critical roles including embryo development,

movement of sperm, migration, and cell growth. Consistent the importance of cilia in embryo

development they host important components of developmental pathways including Sonic Hedgehog

(Shh), Wnt, Hippo, Notch.

Structural and functional defects in cilia result in a number of chronically disabling human pathologies

including autosomal dominant polycystic kidney disease (ADPKD) and complex diseases such as

Joubert syndrome collectively they are called ciliopathy. They display multiple symptoms including

blindness, deafness, heart failure, diabetes, kidney disease, skeletal defects, infertility, obesity and

cognitive impairment. Mutations in over 100 genes have been linked to ciliopathies. However, the

underlying mechanisms of ciliopathy phenotypes resulting from dysfunctional cilia remain poorly

understood.

Due to the importance of cilia for human health there have been a growing interest to identify novel

genes involved in cilia biogenesis. Consistent with intense interest many proteomics and genomics

studies have been conducted and identified about 4000 ciliary candidate genes. However, their roles in

cilia biogenesis could not be extensively examined due to high numbers of putative ciliary genes. Hence

we performed a comparative bioinformatic analysis of proteomics and genomics data for reducing the

number of genes and thus uncovering novel genes involved in cilia biogenesis. Our extensive analysis

revealed a common gene list containing 52 putative ciliary genes. A comprehensive literature search

revealed that 29 genes out of 52 have been already linked to cilia biogenesis. Therefore we focus on

remaining 23 genes which are yet to be investigated in the context of cilia biogenesis. Toward this end

we combine the power of the nematode C. elegans genetics and cell biology with a recently emerged

gene editing technology CRISPR/Cas9 to identify the roles of these genes in cilia biogenesis. We will

present the initial analysis in the meeting.



P.51.RAPID ANEUPLOIDY TESTING IN POSTNATAL VENOUS BLOOD SAMPLES FROM

CUKUROVA REGION

Sabriye KOCATÜRK-SEL1, Mehmet Bertan YILMAZ1, Ümit LÜLEYAP1

Ayfer PAZARBAŞI1, Osman DEMİRHAN1, Davut ALPTEKİN1, Seda ILGAZ1,

Lütfiye ÖZPAK1, İnayet Nur USLU1, Gamze CÖMERTPAY1, Gülsevinç AY1

1Department of Medical Biology, Faculty of Medicine, Çukurova University, Balcali, Adana, Turkey

INNOVATION IN MEDICINE MEETINGS 3, May 11-13, 2017 / The University of Sanko, GAZİANTEP

OBJECTIVE: Postnatal diagnosis of common chromosomal aneuploidies is routinely performed by

standard cytogenetic technique. In this procedure, lymphocyte cells must be cultured for 3 days before

analysis. Therefore, the duration of this process is the major disadvantage for patients who needs quick

diagnosis. A rapid diagnosis has importance for patients with abnormal clinical findings. The

Quantitative Fluorescent PCR (QF-PCR) is rapid, reliable, and sensitive method, based on PCR

amplification of selected chromosome-specific short tandem repeat (STR) markers. It allows the

detection of clinically significant numerical chromosome abnormalities (13, 18, 21, X and Y

chromosomes) in a few hours after sampling. The aim of this study was to determine the postnatal

prevalence of numerical chromosomal anomalies in samples sent with indication of doubt of trisomy

(13, 18, 21), ambiguous genitalia, and sex anomalies (numerical abnormalities of chromosomes X and

Y) in Cukurova Region (South of Turkey).

METHODS: In this study 182 venous blood of postnatal subjects, who were referred to the Department

of Medical Biology, Faculty of Medicine, Cukurova University, Adana/Turkey, between 2010 and 2017,

were analyzed with the QF-PCR technique (AneufastTM QF-PCR Kit, Molgentix SL, Barcelona, Spain).

RESULTS: We detected 101 subjects with trisomy 21 (55,5%), 6 subjects with trisomy 18 (3,3%), 5

subjects with sex anomalies (2,7%) and 70 subjects were normal (38,5%).

DISCUSSION: QF-PCR technique has the advantage of providing rapid results for the diagnosis or

exclusion of aneuploidies of chromosomes 13, 18, 21, X and Y in prenatal and postnatal samples.

Keywords: Aneuploidy, Postnatal diagnosis, QF-PCR, sex anomalies,



P.52.The Expression of CYP2E1 isoenzymes in Breast Cancer, in Relation to Chemotherapy

Arzu Kaya Koçdoğan1, Serpil Oğuztüzün1, Emine Benzer2, Murat Kılıç3, Gülay Dilek2, Yavuz

Selim Kahraman4, Mehmet Ali Gülçelik4, Selin Sayın5

1Kirikkale UniversityFaculty of Science and Arts, Department of Biology, Kırıkkale Turkey

2Dr. Abdurrahman Yurtarslan Ankara OncologyEducationandResearchHospital, Department of

Pathology, Ankara Turkey

3Ankara UniversityVocational School of Health Services, Department of Pharmacy Services, Ankara

Turkey

4Dr. Abdurrahman Yurtarslan Ankara OncologyEducationandResearchHospital, Department of General

Surgery, Ankara Turkey

5İskenderun Technic University, Marine Science and Technology Faculty, Iskenderun-Hatay, Turkey

Breast cancer is the most common cause of cancer related deaths in women. Environmental chemicals

are one of the risk factors in breast cancer genesis. Cytochrome P450 (CYP) enzymes play a major role

in the activation of these chemicals. Of environmental factors, especially polycyclic aromatic

hydrocarbons (PAHs) have been suggested to play a causative role in breast cancer etiology. In this

study, the protein expressions of CYP2E1 isoenzyme will be investigated in chemotherapy-treated and

non-treated breast cancer patients’ tissues. The expression differences between two groups were

examined statistically. The expression of CYP2E1 isoenzymes were studied by immunohistochemistry

method from 50 chemotherapy-treated and 95 non-treated breast cancer patients’ tissues and peripheral

to tumor tissue as a control. The CYP2E1 expression were higher in tumor epithelium than that in

normal epithelium in breast cancer patients’s tissues. There was not any statistically significant

differences CYP2E1 expression in the tumor and normal epithelium of the chemotherapy-treated and

non-treated breast cancer patients’ tissues (p>0.05). There were not any statistically significant

diferences between the CYP2E1expression and clinical information (estrogen receptor status,

progesterone status, tumor grade, smoking status, patient’s age) (p>0.05). In conclusion, CYP2E1

isoenzyme may play a role in development and tumorogenesis of breast cancer.

Key words: Breast Cancer, CYP2E1, Immunohistochemistry



P.53.MORPHOLOGICAL AND CHEMICAL CHARACTERIZATION OF SPIRULINA

PLATENSIS TO SYNTHESIS OF PROTEIN-BASED MEDICAL GRADE BIOPOLYMERS

S. SAYIN3, T. DEPCİ1, M.NAZ3,K.O. YAKINCI1, M.E.YAKINCI2




Abstract

The aim of this study is to make morphological and chemical characterization of the Spirulina

platensis (Cyanophyta) which is a cyanobacteria type, grown at Iskenderun Technical University, Algal

Bioechnology Laboratory in order to base for future studies related with the synthesis of protein-based

medical grade biopolymers. The morphology, structure and property of S.platensis were analyzed by

quantitative method, XRD, FTIR, BET and SEM respectively. The protein, oil and carbohydrate

contents of S.platensis were determined as 69.3 %, 6 % and 17 %, respectively.

The XRD pattern indicated that the S.platensis had amorphous structure, as expected and wide

curve between 15°and 25° 2θ values indicated the lignocelluloses materials. The bands recorded in IR

spectrum represented the C=C, C=O vibrations and generally aromatic structure. The BET surface area

of S.platensis was found very low (3.2 m2/g) and this result was supported by SEM images. High

protein contents and preliminary experiments done by us point out that S.platensis is viable candidates

for the synthesis of bio-composite medical grade polymers mixed with PLA, PEG and PVA.

Key Words: Spirulina platensis, Chemical characterization, Bio-composite, Polymers, Medical



P.54.COMPARISION OF LENS DOSES USING VOLUMETRIC MODULATED ARC

THERAPY AND FIELD IN FIELD RADIOTHERAPY PLANNING TECHNIQUES IN WHOLE

BRAIN IRRADIATION OF PATIENTS WITH BRAIN METASTASES

Sinan DUMAN1, Can DEMİREL2 1Kahramanmaraş Necip Fazıl Hospital, Department of Radiation Oncology, Kahramanmaraş,

[email protected] 2Gaziantep University, Department of Biophysics, Gaziantep, [email protected]

Purpose: Our study included comparison of lens doses using volumetric modulated arc therapy (VMAT)

and field in field (FIF) radiotherapy planning techniques in the treatment of whole brain radiotherapy of

patients with brain metastases.

Method: VMAT and FIF radiotherapy planning techniques were applied on the computed tomography

(CT) images of 20 patients with brain metastases. Target volume is planned with daily fractional dose of

3 Gy, 30 Gy in total for palliative purposes. The plannings were designed, all of the target volumes

receive 95% of the defined dose. The maximum, minimum and mean dose values of the right and left

lenses obtained from the treatment plan were examined statistically.

Evidences: Comparing the VMAT and FIF radiotherapy planning techniques, it was found that the

maximum and average dose values of the lenses were lower in the FIF technique. (p=0.001)

Discussions: As a result of treatment planning using VMAT and FIF techniques in whole brain

radiotherapy, FIF technique was statistically better in terms of target conformality index and lens doses.

Result: In the paliative treatments, priority is the increasing life quality of patients with brain metastases

in whole brain radiotherapy therefore lens doses are of great importance in this sense. Lenses are

preserved better using field in field technique so that this technique is recommended in using clinical

practice.

Keyword: VMAT, FIF , CI, Whole Brain Radiotherapy, Lens



P.55.SYNTHESIS OF CHARACTERIZATION OF CURCUMIN BASED INJECTABLE

HYDROGELS

Büşra Aksoya, Süleyman Köytepea, Burhan Ateşa, Ilgın Türkçüoğlu b, Meltem Kuruşc

aDepartment of Chemistry, İnönü University, 44280-Malatya, Turkey

bDepartment of Obstetrics and Gynecology, İnönü University School of Medicine, Malatya, Turkey

cDepartment of Histology and Embryology, Izmir Katip Celebi University, İzmir, Turkey

Email: [email protected]

Curcumin is a naturel molecule, also known as diferuloyl methane and has two aromatic ring

systems containing o-methoxy phenolic groups, connected by a seven carbon linker consisting of an α,β-

unsaturated β-diketone moiety[1]. Curcumin is an important molecule to protect humans from chronic

health. Hydrogels represent an important class of biomaterials that are used in lots of biomedical

applications such as tissue regeneration[1] cell delivery therapies[2] but also biosensors[3] and drug

delivery systems[4]. In this study, curcumin based hydrogels were synthesized from cyclodextrin,

curcumin and different molecular weight polyethylene glycol for biomedical applications. The ratios of

PEG–curcumin monomer units in the polymer were: 95:5, 90:10, and 85:15, respectively. Structural

characterization of curcumin based hydrogels was characterized by FTIR, DSC, SEM, TGA and

dynamic contact angle measurements. The prepared hydrogels exhibited good flexibility, suitable pore

distribution and high thermal stability. With increasing the curcumin content of the structure of the

hydrogel, the stability of the polymers increased. In addition, biocompatibility properties were

determined for curcumin-based hydrogel. The prepared curcumin-based hydrogels give then a high

specific surface area that is available for drug releasing systems.

This work was supported by Scientific and Technological Research Council of Turkey

(TÜBİTAK) with project number 215Z322.

References

[1] J. L. Drury, D. J. Mooney, Biomater. 2003, 24, 4337-4351.

[2] M. P. Lutolf, J. A. Hubbell, Nature Biotechnol. 2005, 23, 47-55.

[3] Y. Guan, Y. Zhang, Soft Matt. 2011, 7, 6375-6384.

[4] S. C. Lee, I. K. Kwon, K. Park, Adv. Drug Delivery Rev. 2013, 65, 17-20.



P.56.DEVELOPING A PLATFORM INDEPENDENT WEB APPLICATION FOR ANALYSING

AND VISUALIZING THE STRUCTURAL PROPERTIES OF BIOMOLECULES

Şeyma YAMAN1, Ferhat AYAZ1 and Hüseyin KAYA1

1Gaziantep University, Faculty of Health Sciences, Department of Bioinformatics and Computational

Biology 27310 Şehitkamil, Gaziantep Türkiye

Understanding the relationship between sequence-structure-function of biomolecules plays a major role

in diagnosing and treating many diseases at the molecular level. For this reason, there is a huge demand

for developing computer softwares, which provides three-dimensional visualization of functionally

active and/or stable protein structures [1].

In this study, it was aimed to develop a new web application with the help of computer graphics for

visually examining the structural properties of proteins. The web application, which is still under

development, is a platform independent application and, therefore, will be compatible with all modern

web browsers, computers, and mobile devices. For this purpose, WebGL, an interface of the OpenGL

graphics library, and a dynamic programming language, JavaScript, are used. The jQuery and

THREE.js libraries have also been benefited to speed up and simplify the use of JavaScript.

The current version of our web application is capable of visualizing three-dimensional structure of

proteins and provides the information about the physicochemical properties of the amino acids in that

protein. When this web application is completed; it may help to make structural and functional analyses

much easier and more understandable in researches conducted at the molecular level in the field of

health and biological sciences [2].

References

[1] N. Rego and D. Koes, “3dmol.js: molecular visualization with WebGL,” Bioinformatics, vol. 31, no.

8, pp. 1322–1324, Apr. 2015.

[2] C. Mura, C. M. McCrimmon, J. Vertrees, and M. R. Sawaya, “An Introduction to Biomolecular

Graphics,” PLoS Computational Biol- ogy, vol. 6, no. 8, p. e1000918, Aug. 2010.



P.57.DETERMINATION OF THE EFFECTS OF SEED EXTRACT (URTİCA DİOİCA L.) ON

IRRADIATED RATS BRAIN TISSUES

Kenan YILDIZHAN1, Ömer Can DEMİRTAŞ2, Ahmet UYAR3, Zübeyir HUYUT4,

Tahir ÇAKIR1, Zabit YENER3, Ömer Faruk KELEŞ3

1 Yuzuncu Yil University, Faculty of Medicine, Department of Biophysics, Van, Turkey 2 Gaziantep University, Faculty of Medicine, Department of Biophysics, Gaziantep, Turkey 3 Yuzuncu Yil University, Faculty of Veterinary, Deparment of Pathology, Van, Turkey 4 Yuzuncu Yil University, Faculty of Medicine, Department of Biochemistry, Van, Turkey

Aim: Ionizing radiation leads to side effects on non-targeted tissues through increasing the oxidative

stress during radiotherapy. Our study was to investigate the protective effect of Urtica dioica L. seed

extract (UDSE) against radiation-induced oxidative damage in brain tissue.

Material Methods: 32 rats that were eight weeks, divided into 4 group(n:8). Control group(C); was fed

with pellet for 10 days. Radiation group (IR) was fed with pellets for 10 days after exposing 5Gy

radiations as a single fraction. Radiation+UDSE (IR+UDSE) group was exposed 5Gy radiations as a

single fraction and was fed with UDSE for 10 days. UDSE group (UDSE) was fed with only UDSE for

10 days. After the anesthesia (50 mg/kg ketamine and xylazine) was administered, rats were sacrificed.

We determined the levels of MDA, SOD, CAT, GSH and GSH-Px enzymes and examined the

histopathological findings in brain tissues.

Results: Radiation group had higher MDA level compared to other groups, while SOD level was lower

(p<0.05). MDA, SOD, CAT, GSH and GSH-Px levels of radiation+UDSE group were similar

compared to the control and UDSE groups (p>0.05). Histopathological examination of brain tissues

showed no significant change between groups.

Conclusion: These results showed that irradiation (5Gy) elevated the level of MDA via increasing the

lipid peroxidation and decreasing the antioxidant capacity. On the other hand, after exposure to radiation

the administration of UDSE, has antioxidant properties, may reduce the oxidative stress damage caused

by radiation. However, 5Gy radiation dose did not cause any pathological degeneration in brain tissues.



P.58.SYNTHESIS OF CALCIUM PHOSPHATE BIOMATERIALS FROM SEA URCHIN

SKELETON AND COMPARASION WITH BONE CEMENT

T. DEPCİ1, B. BOZYİĞİT4, K.O. YAKINCI1, S. SAYIN3, M.E.YAKINCI2




4 Gözde Akademi Hastanesi, Malatya.

Marine species are generally composed of pure calcium carbonate with small amount of

impurities and readily available and abundant. However they have yet to be enough investigated for

their possible applications or potentials as a source of value-added components. In the present study,

calcium phosphate was synthesized from sea urchin skeleton by modified wet-chemical precipitation

route to use as an alternative material for bone cement. The obtained compound was characterized by

XRD, FTIR, DTA, SEM, ICP-MS, particle size analyzer and magnetometer. The results showed that the

calcium phosphate was identified as nano size whitlockite (Ca2.86Mg0.14(PO4)2) which has homogenous

phase with nano size of about 100 nm. In addition, its heavy metal contents (Sr:1790787.415 ppb, Fe:

94066.166 ppb, Al: 486852.232 ppb, Zn: 2139.53 ppb) can be neglected when compared with

hydroxyapapite produced from other marine species. The hysteresis loop belonging to whitlockite

indicated the magnetic property as a result of the magnesium content (2.5%) of the sea urchin skeleton.

In order to compare the commercial bone cement, the whitlockite was mixed with methyl

acrylate and filled into animal tibia. When commercial bone cement solidified, the temperature rose to

75 degrees which is very hazardous for both doctors and patients. On the other hand, the mixture of the

whitlockite and methyl acrylate solidified in 8 minutes without any temperature rise (23.5 degrees). In

addition, the mixture can be easily seen in the tibia under X-ray without BaSO4 which is 10 percent ratio

in commercial bone cement, providing visibility under X-ray.

Key Words: Sea urchin, Calcium phosphate, Bone cement, Whitlockite



P.59.CARDIAC TISSUE REGENERATION WITH HUMAN ADIPOSE-DERİVED

MESENCHYMAL STEM CELLS ON 3D SILK FIBROIN SCAFFOLDS

1*Yuksel CETİN, 2Merve GIZEM SAHİN, 2Fatma Neşe KÖK

1*TUBITAK MRC, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli

1Istanbul Technical University, Department of Moleculer Biology and Genetic, Maslak Istanbul

([email protected])

Cardiovascular disease is the leading cause of morbidity and mortality worldwide. Human heart tissue

does not regenerate spontaneously and has a very limited innate capacity for repair, thus “regenerative

medicine” represents a promising alternative therapy. Cardiac tissue engineering aims to deliver the

cardiac-like cells, pluripotent cells or progenitor, biomaterials, and signaling factors to the damaged

cardiac tissue for cardiomyocyte regeneration, and neovascularization. The aim of this study was to

produce biocompatible, biodegradable, and strengthful 3D biomaterial and to investigate its use with

human adipose derived mesenchymal stem cells (hAD-MSCs) for in vitro cardiac tissue regeneration.

3D silk fibroin scaffold was produced and water uptake and biodegradation capacity analized then the

surface morphology and porosity was characterized with SEM analysis. MSCs from human adipose

tissue was isolated by collagenase. hAD-MSCs was characterized by flow analysis of specific positive

cellular markers such as CD44, CD73, CD13, anti-human CD90, anti-human CD166, CD105, CD13 and

negative cellular markers such as CD11b, CD34, CD15, CD14, CD19, CD45, anti HLA-DR. The

biocompatibility of 3D silk fibroin scaffold using hAD-MSCs was evaluated by performing WST-1,

MTT, and Live/Dead assays following 1, 7 and 14 days incubation periods. The cardiomyocytes

differentiation potential of hAD-MSCs on 3D silk fibroin scaffold was examined by

immunofluorescence staining of cardiac biomarkers: α-actinin, Troponin I, Connexin 43, and Myosin

heavy chain. As a result of this study, the produced 3D silk fibroin has a good biocompatibility,

biodegradation rate, suitable mechanical strength and it provides cardiomyogenic differentiation of

hAD-MSCs in the in vitro system.

Key words: Cardiac Tissue Engineering, 3D Silk Fibroin, Mesenchymal Stem Cells, Cardiac

Regeneration



P.60.ASSESSMENT OF TRIPODAL AMINOPHENOLATE FE-COMPLEXES CONTAINING

PHEN OR 8HQ AS ANTICANCER AGENTS

Zelal ADIGUZEL1, Yasemin YILDIZHAN1, Cristina P. MATOS2, Isabel CORREIA2, Joao Costa

PESSOA2, Yuksel CETIN1, Ceyda ACILAN1

1 TUBITAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Kocaeli,

TURKEY

2 Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, PORTUGAL

AIMS: We report the design and preliminary studies on a family of Fe(III) complexes with a tripodal

aminophenolate ligand and i) NN-heterocycles or ii) hydroxyquinoline derivatives. The underlying

principle is that metal ion homeostasis of Fe will be better dealt by human physiology and cause less

damaging side effects than Pt-compounds. The aim is to obtain stable, efficient and selective Fe-

complexes that may be used as anti-cancer agents.

METHODS: The compounds have been characterized in the solid state and in solution by elemental

analysis, ESI-MS spectrometry, 1H and 13C-NMR, FTIR and UV-vis absorption spectroscopy. Cell

viability was measured via MTT and WST-1 analyses in three different cell lines. For selected

compounds with promising cytotoxic activity, apoptosis was evaluated using cell and DNA

morphology. DNA damage was assessed via in-vitro plasmid assay, and ɣH2AX and 8-oxo-Guanidine

staining in cells and comet assay.

RESULTS: While there were differences between distinct cell lines, all complexes were cytotoxic at

24h, which was further elevated by 72h. The mean of cell death appeared to be apoptosis. The

compounds induced oxidative DNA damage on plasmid DNA and in cell culture. Comet assay

confirmed presence of genomic damage. There were also increased DNA double strand breaks, which

may be the mechanism of action of these compounds under study.

DISCUSSION: Three compounds were selected amongst a panel of nine drugs for further studies, and

they seemed to use similar cell death pathways and mechanism of action. Initial studies showed

promising activity granting premise for further studies.



P.61.INVESTIGATION OF IN VIVO EFFECTS OF ARACHIDONOYL DOPAMINE ON LIPID

PEROXIDATION AND SOME ENZYME ACTIVITIES IN RAT TISSUE AND

ERYTHROCYTES

Zübeyir HUYUT1, Şükrü BEYDEMİR2, İlhami GÜLÇİN3, Mehmet Tahir HUYUT4

1Department of Biochemistry, Medical Faculty, Yuzuncu Yıl University, Van, Turkey 2Department of Biochemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey

3Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, Turkey 4Department of Bioistatistics, Medical Faculty, Yuzuncu Yıl University, Van, Turkey

Aim: Phenolic compounds are widely used in many foods and drugs, because of their antioxidant

properties. Arachidonoyl-dopamine, a phenolic compound, is an endogenous lipid of central nervous.1,2

We investigated effect of arachidonoyl-dopamine on MDA, GSH, GSH-Px, catalase and carbonic

anhydrase (CA) activities in erythrocytes (RBCs), kidney, heart and eye of rats, time-dependently.

Method: Adult male rats (weighing 200-250 g) were divided into 4 groups (n=6). Group 1 and 2 were

administered intraperitonal blank-injection (1st and 4th hours sham groups, respectively). Groups 3 and 4

were administered 10 mg/kg arachidonoyl-dopamine intraperitoneally. One hour later groups 1 and 3,

and 4 hours later groups 2 and 4 were sacrified under anesthesia. MDA and GSH levels, GSH-Px, CAT,

CA activities were measured in RBCs, kidney, eye and heart tissues, according to previously defined

methods, respectively.3-7

Results: There was no statistically significant difference between the parameter values of sham groups

at 1 and 4 hours in all tissues (p>0.05). MDA levels of all tissues were decreased compared to sham

groups, while GSH, GSH-Px, CAT values were increased in the 1st and 4th hours arachidonoyl-

dopamine groups (p<0.05). In addition, CA activity of the 3st and 4th groups were decreased with

according to sham groups in all tissues, time-dependently (p<0.05).

Discussion: These results showed that arachidonoyl-dopamine increased antioxidant enzyme activities,

and inhibited lipid peroxidation and CA enzyme activities during four hours. Therefore, it may be used

as a medicine the oxidative stress-inhibiting and for treatment of diseases such as glaucoma associated

with CA, in future.

Key Words: Arachidonoyl-dopamine, antioxidant enzymes, carbonic anhydrase, glokom, lipid

peroxidation, oxidative stress.

References:

1- Huyut Z, Beydemir Ş, Gülçin İ. Inhibitory effects of some phenolic compounds on the activities

of carbonic anhydrase: from in vivo to ex vivo. Journal of Enzyme Inhibition and Medicinal

Chemistry 2016; 31(6): 1234-1240.

Marinelli S, Di Marzo V, Florenzano F, Fezza F, Teresa Viscomi M, Van der Stelt M, Bernardi G,

Molinari M, Maccarrone M and Mercuri NB. N-Arachidonoyl-Dopamine

http://www.tandfonline.com/author/Huyut%2C+Z%C3%BCbeyir

http://www.tandfonline.com/author/Beydemir%2C+%C5%9E%C3%BCkr%C3%BC

http://www.tandfonline.com/author/G%C3%BCl%C3%A7in%2C+%C4%B0lhami



P.62. COMPUTATIONAL DESIGN, PREPARATION AND CHARACTERIZATION OF

IMMUNOLOGICAL PEPTIDE OF TOXOPLASMA GONDII MAJOR SURFACE PROTEIN

Rabia Cakir Koc1, Yasemin Budama-Kilinc1, Serda Kecel-Gunduz2*, Yagmur Kokcu2

1Yildiz Technical University, Department of Bioengineering

2*Istanbul University, Department of Physics

ABSTRACT

Toxoplasma gondii is one of the most widely spread parasitic organisms in the world. T.gondii causes

primary, chronic infection and mortality. Major surface antigen 1 (SAG1) is the most abundant

tachyzoite surface protein and highly conserved between species and causes strong humoral response.

Therefore SAG1 is good candidate for vaccine purpose. Especially immunogenic peptides gains

importance for new generation vaccine development. Some studies showed that this peptide sequence

has immunity. To understand how a molecule may bind to a target molecule and how the two molecules

may interact, computational chemistry is used as an effective method to perform drug design.

Computational chemistry provide important information on structure-activity relationship, biological

effects of functional groups, molecule geometry, design of enzyme inhibitors and agonists. Molecular

mechanic (MM) and molecular dynamics (MD) calculations would be preferable to investigate

conformational variation for peptide molecule that one uses as an ingredient of the drug. Molecular

Dynamics (MD) simulation is also applicable tool to understand protein folding and unfolding dynamics

and gives important interaction between the atoms of the protein and the solvent. The interaction of

drugs with protein systems was carried out by means of computing the free energy of binding using the

molecular docking technique.This study we aimed the computational design and encapsulation of

immunogenic peptide of T.gondii with Poly-Lactic-co-glycolic acid (PLGA) as vaccine candidate.

Keywords: computational design, molecular dynamics, toxoplasma, peptide, poly lactic co glycolic acid

(PLGA)

Fig. 1.The ligand(Ile-Cys- Pro-Ala-Gly-Thr-Thr-Ser-Ser-

Cys-Thr-Ser-Lys-Ala-Val-Thr-Leu-Ser-Ser-Leu) is connected to the active site of MHC protein.



AUTO INDEX

A-F

Akram Mudher Ahmet Ulu

Aziz Yarbil Ayfer Pazarbaşı

Badr Q. Ismael Ayşeğül Hanikoğlu

Bahadır Batar Ayşe Burcu Çehreli

Barbaros Şahin Karagün Ayşe Nur Sarı

Beste Turanlı Ayşegül Çalışkan

Burçin Nilay Yener Ayşegül Gölcü

Cem Varan Bahadır Batar

Cumhur Kırılmış Barış Özgür Dönmez

Emin Türkay Korgun Bayhan Karabulut

Emine Yavuz Bircan Çeken

Erdal Uysal Birgül Köstek

Ergün Mendeş Burak Şentürk

Ertuğrul Allahverdi Burcu Bayel Seçinti

Eyüphan Yakıncı Burçin Türkmenoğlu

Fatma Kübra Tombultürk Büşra Aksoy

Filiz Gülşen Damla Kurtunluoğlu

Deniz Özen Ebru Kuyumcu

Efe Sezgin Elif Şen

Ensar Erel Erdal Uysal

Esra Bozgeyik Filiz Koç

Gizem Gülfidan

G-K

Gamze Varan Gökhan Görgişen

Gülşen Öztürk Gülten Kavak Balcı O-31



G-K

Günnur Koçer Hatice Gamze soğukömerlioğulları

Hatice Aygün Hamit Hakan Alp

Hülya Çiçek İsmail Kaşoğlu

İbrahim Bozgeyik İlknur Öztürk Özgençli

İmren Özcan İsmail Musab Gülaçar

İncilay Gökbulut Khatab Adnan

Kazım Yalçın Arga

L-R

Marina Oruç Mesut Çay

Mecit Özdemir Murat Karabaş

Mehmet Demir Mustafa Pehlivan

Merve Gökşin Karaaslan Nurcan Ayhan

Muhammed Karaman Nazlı Erdoğar

Mustafa Çiçek Rozghar Abdula

Naser Gilani Pelin Balçık Erçim

Nurcan Karaman Öznur Doğan Ulu

Özlem Demirci



S-Z

Samir Abbas Saadet Özen

Serkan Gürgül Sabiha Çevik

Sevgi Balcıoğlu Sabriye Kocatürk

Süray Pehlivanoğlu Selin Sayın

Serda Kecel Gündüz Şafak Özhan

Sinan Duman

Tahir Çakır Süleyman Köytepe

Şafak Özhan Kocakaya Şeyma Yaman

Tolga Depçi Yüksel Çetin

Yücel Başpınar Zübeyr Huyut

sanko university innovation in medicine summit-3, 11-13 may … · 2017. 6. 13. · sanko...

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