sample preparation catalog - hplc columns … preparation catalog 2 dear customer we are pleased to...

SPE cartridges, on-line SPE columns, QuEChERS

and associated products

based on

Molecularly Imprinted Polymers (MIP) sorbents

Polymeric sorbents

Modified Silica sorbents

2015

SAMPLE PREPARATION

CATALOG

2

Dear Customer

We are pleased to present the 2015 AFFINISEP sample preparation Catalog containing innovative

solutions to help you to achieve your goals. This year, we launch several innovative products for new

applications including our AFFINIMIP®SPE Multimyco LCMSMS dedicated to analysis of

multimycotoxins (including DON) before LC-MS/MS and AFFINIMIP®SPE GLYPHOSATE – AMPA. We

invite you to regularly visit our application notebook at www.affinisep.com that can help you in your

challenges.

At AFFINISEP,

Our R&D team is fully committed to developing quality analytical and diagnostic products that help

chemists get accurate information as simply as possible;

Our manufacturing organization supported by highly skilled sales and support teams is at your

disposal to satisfy you.

Your satisfaction is our engine.

Kaynoush Naraghi

CEO

TECHNICAL SUPPORTAFFINISEP has fully integrated technologies platform with specialized teams in organic chemistry,polymer chemistry, analytical and bioanalytical chemistry who are at your disposal to help you inyour challenges.

At AFFINISEP, we are committed to providing the best technical support possible. Our TechnicalSupport Group is a team of highly qualified M.Sc. and PhD Chemists, who are at your disposal toresolve your problem and to answer to your queries. For technical inquiries, feel free to contact useither by email: [email protected]

We are also very thankful to customer’s feedback about our products, protocols and customerservices by email to: [email protected]

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SOLID PHASE EXTRACTION – PRODUCT SELECTION GUIDE 4SORBENTS 5SOLID PHASE EXTRACTION 6QUICK LOOK-UP GUIDE 7Examples of SPE APPLICATIONS 8Formats available for SPE applications 11

AFFINIMIP®SPE (MOLECULARLY IMPRINTED POLYMER) 13AFFINIMIP® SPE – CONCEPTS 14AFFINIMIP® SPE FOR ANALYTICAL PURPOSE 15

Multimyco LCMSMS for Multimycotoxins analyses NEW! 17Fumonisins and Zearalenone (simultaneously) 18Zearalenone 19

Ochratoxin A 20Patulin 21Deoxynivalenol 22Estrogens 23Bisphenol A 24Amphetamines 25Chloramphenicol 26Tetracyclines 27Tamoxifen 28Zeranol residues 29Picolinic herbicides, Picloram, Aminopyralid, Clopyralid 30Glyphosate – AMPA NEW! 31Catecholamines 32

Metanephrines 33

Phenolics 34AFFINIMIP® SPE product list 35

AttractSPETM POLYMERIC CARTRIDGES 37

SELECTION OF THE AttractSPETM CARTRIDGES 38AttractSPE™ W/O -HLB - Hydrophilic Lipophilic Balance 39 AttractSPE™ DVB - Reversed Phase 39AttractSPE™ WAX - Weak Anion Exchange 39AttractSPE™ WCX - Weak Cation Exchange 40AttractSPE™ SAX - Strong Anion Exchange 40AttractSPE™ SCX - Strong Cation Exchange 40

Polymeric AttractSPETM CARTRIDGES – Product List 41

SilactSPETM SILICA BASED SORBENT SPE CARTRIDGES 43SilactSPETM SILICA-BASED SPE CARTRIDGES – Product List 46

On-Line SPE 49QcleanupTM - QuEChERS 51MYCOTOXINS STANDARDS 55SPE ACCESSORIES 57

CUSTOM-MADE PRODUCTS & SERVICES 59PROJECT DEVELOPMENT 61

ABOUT AFFINISEP 64ORDERING INFORMATION 64

TABLE OF CONTENTS

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SOLID PHASE EXTRACTION PRODUCT SELECTION

GUIDE

4

Sample preparation and Clean-up are the key steps in trace analysis for analytical chemist.

As specialist in this field, AFFINISEP supplies a complete range of solutions for sample

preparation based on Solid Phase Extraction process.

Solid phase extraction (SPE) is a powerful technique to provide a rapid and selective sample

clean-up, a high recovery and the concentration necessary for accurate quantitative analysis.

A multitude of phase chemistries and formats are available in this catalog for various

applications. We offer a comprehensive range of SPE sorbents to give you all elements to

face the increasingly complex and diverse sample preparation challenges to:

Simplify data analysis by removing interferences

Increase sensitivity and reliability by enrichment of the analyte

Obtain high and reproducible recovery yields from complex samples

In order to help you to select the appropriate product, you can check our Application

notebook which gathers a broad variety of analysis.

With this large number of phase chemistries available, different formats can be used:

•Open and reversible Solid Phase Extraction (SPE) cartridges or 96-wells plate for

automated or manual SPE

•On-line SPE columns for a direct coupling on a LC analysis. This format is very useful for

small sample extract or very sensitive analysis.

•QuEChERS with dispersive SPE or SPE cartridges for the clean-up process. This method was

mainly developed for the analysis of a high number of pesticides.

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SORBENTS

5

SPE Sorbents

AFFINISEP offers a complete range of sorbents for solid phase extraction from very specific

to a crude clean-up phases, from silica to polymers, from conventional to more sophisticated

sorbents for various applications.

AFFINIMIP® SPE

AFFINIMIP® SPE is a selective solid phase extraction based on Molecularly Imprinted

Polymers (MIP) that combines the advantages of immune-affinity columns regarding the

selectivity and those of polymeric SPE sorbents in terms of costs and robustness. Developed

for very problematic analyte purification, an instruction sheet gives all required information

to successfully carry out the analysis, including the protocol. No method development is

required. The protocol is easy, simple and fast. The chromatogram analysis is fast thanks to

the affinity between the analyte and AFFINIMIP® SPE. Methods are developed for the usual

analyte containing matrices. These matrices can be as diverse as aqueous matrices (food,

feed and biological matrices), fully organic matrices (oil), powder (milk) or cereals grains. If

your sample preparation is complex or gives inappropriate results, the development of an

AFFINIMIP® SPE can be a solution.

AttractSPETM Polymeric sorbents

AttractSPETM are the last generation of polymeric SPE sorbent. They are crosslinked polymer

particles bearing various chemical functionalities. A limited number of sorbent (6 products)

simplifies your choice and covers the full range of analysis using aqueous matrices.

These sorbents provide all the advantages of polymeric sorbents, including robustness (to

solvent, pH and T), simplified method development, wide applicability and not affected by

drying out. The combination of the water-wettable optimised surface chemistry, high

surface area and pH stability ensures high reproducible recoveries for a wide range of

analytes. For the same application, less sorbent and less solvent are required than for silica-

based sorbent and so, their added value is particularly obvious for trace analysis and for

small volume sample.

SilactSPETM Silica-based sorbents

The first sorbents historically used for SPE applications, Silica-based sorbents offer a broad

range of chemically modified sorbents. This chemistry goes from very polar sorbent (bare

silica) to hydrophobic sorbent (end-capped saturated hydrocarbon modified silica) passing

through intermediate polarity (for instance, amino modified silica). These sorbents are

sensitive to extreme pH. They are adequate for non-polar analytes in simple matrices.

QcleanupTM QuEChERS

QcleanupTM dispersive SPE products are a mixture of Magnesium Sulfate, PSA, Black carbon

or C18 used by QuEChERS method for the clean-up of fruits and vegetables during

multiresidues pesticides analyses.

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SPE PROCEDURE STEPS

Loading Washing Elution

Analytes of interest

Solid phase extraction requires the following steps:

1- Sample preparation: This step is necessary to obtain a loading solution compatible with

retention condition in the SPE column. In case of solid matrices, this solution must also

extract analytes from these matrices.

2- Conditioning: The SPE cartridges are conditioned with the appropriated solvents to fully

wet sorbent and enable further interactions between the analytes and the sorbent.

3- Loading: the percolation solution is loaded through the SPE cartridge. The analyte must

be retained in the column as well as unwanted compounds.

4- Washing: interferents and unwanted compounds are washed off by using several

solvents.

5- Elution: The desired analyte is extracted from the SPE cartridge.

Retention Mechanism

SPE cartridges are available in a complete and wide range of sorbents. A sorbent is often

classified according to the main retention mechanism between the analyte and the sorbent

(normal phase, reversed phase, ion-exchange, mixed-mode).

Normal phase retention mechanisms are based on polar-polar or dipole-dipole interactions

between the analyte and a silica-based sorbent.

Reversed phase retention mechanisms are based on non polar- non polar interactions and

Van der Waals dispersive forces. The sorbent is hydrophobic (polymeric sorbent or alkyl or

phenyl modified silica-based sorbent).

Ion-exchange retention mechanism use electrostatic interaction between a charged sorbent

and the ionic analyte. The sorbent is charged with the opposite charge of the analyte. A

cationic (respectively anionic) analyte interacts with a cation (respectively anion) exchange

sorbent.

Mixed-mode sorbents mainly interact through reversed phase and ion exchange retention

mechanisms. They are available as a polymeric sorbent (AttractSPETM SAX, WAX, WCX or

SCX) or as SilactSPETM.

SOLID PHASE EXTRACTION

www.affinisep.com 7

Analyte of interest

Existing AFFINIMIP® SPE

Selection of a sorbent

YESProblem solved

Ready to use Protocole

NO

AqueousNature of the

matrixWater miscible organic solvent

Not water miscible organic solvent

Nature of the analyte

ionic NeutralPolar to moderately

polarModerately to

Non Polar

Ion-Exchange AttractSPETM AttractSPETM DVB

Normal phase SilactSPETMAttract SPETM W/O - HLB

Sorbent

The choice of the sorbent is crucial for a successful sample pre-treatmentWhen AFFINIMIP®

SPE exists for the analyte, the choice is very simple and the problem is solved. The protocol

is simple and ready to use. AFFINIMIP® SPE have been developped for various trace analyses

in complex matrices and for problematic analysis with common SPE sorbents.

When no AFFINIMIP® SPE product exists, you can check the application notebook available

on our website to use the appropriate sample pretreatment.

In other cases, the sorbent chemistry must be chosen very carefully and each step of the

protocol must be optimized. This choice is based on the nature of the analyte and of the

matrix, on the loading condition and on the planned retention mechanism with the sorbent.

The following scheme gives a brief guideline to select the family of sorbents.

For specific applications such as trace analysis or complex matrices, conventional SPE

sorbents may give very complex analysis or unsatisfaying results. The development of an

AFFINIMIP® SPE sorbent for this application is a solution. We provide as well services for

method development.

QUICK LOOK UP GUIDE

For multiresidues analyses like pesticides, QcleanupTM QuEChERS and AttractSPETM W/O – HLB can beused.

Check the application notebook

www.affinisep.com

Analyte

Family SPE product- ANALYTES SPE product- ANALYTES MATRICES REF PAGE

Multimycotoxins

Aflatoxins, Ochratoxin A, HT-2, T-2,

Fumonisins, Zearalenone,

Deoxynivalenol

AFFINIMIP® SPE

Multimyco LCMSMSCereals FS118 17

Fumonisins AND ZearalenoneAFFINIMIP® SPE

FumoZON

Maize, Maize-based baby

foodFS109 18

Single Mycotoxin

ZearalenoneAFFINIMIP® SPE

Zearalenone

Maize, Wheat, Cereal-

based baby food, Rice,

Edible corn oil

FS100 19

Ochratoxin AAFFINIMIP® SPE

Ochratoxin A

Wheat, Maize, Pepper,

Paprika, Red and White

Wine

FS101 20

Patulin AFFINIMIP® SPE Patulin

All Apple-based products

(Juice, puree,

concentrate…)

FS102 21

Deoxynivalenol (DON)AFFINIMIP® SPE

DeoxynivalenolWheat, Maize, Oat FS117 22

Estrone, 17α-Estradiol, 17β-Estradiol,

Estriol, 17α- Ethynilestradiol

AFFINIMIP® SPE

Estrogens

Water, Serum, Plasma FS10423

Bisphenol A, Bisphenol AP, Bisphenol

AF, Bisphenol B, Bisphenol S, Bisphenol

F…

AFFINIMIP® SPE

Bisphenol A

A broad variety of liquid

and solid foodsFS106 24

ParabensAFFINIMIP® SPE

PhenolicsShampoo, cream FS103 34

Phenolic compoundsAFFINIMIP® SPE

PhenolicsFood matrices FS103 34

Amphetamine, Methamphetamine,

MDA, MDMA, MDEA

AFFINIMIP® SPE

AmphetaminesSerum, Urine DG102 25

Zeranol, Zearalanone, α and β

Zearalanol, α and β Zearalenol,

Resorcylic acid lactones

AFFINIMIP® SPE

Zeranol ResiduesUrine, Plasma FS105 29

ChloramphenicolAFFINIMIP® SPE

ChloramphenicolHoney, Urine, Shrimp FS110 26

TamoxifenAFFINIMIP® SPE

TamoxifenUrine PH101 28

Examples of SPE APPLICATIONS

8

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See our application notebook for more applications and details…

www.affinisep.com

ANALYTE

FAMILY SPE product- ANALYTES SPE product- ANALYTES MATRICES REF PAGE

Nicotine, Procaïnamide AttractSPETM W/O - HLB Urine HLB 39

Caffeine AttractSPETM W/O – HLB Urine, Water HLB 39

Propranolol AttractSPETM W/O - HLB Urine, Water HLB 39

Tetracyclines - Tetracycline, Oxytetracycline,

Chlortetacycline, Doxycycline

AFFINIMIP® SPE

TetracyclinesMilk FS112 27

Sulfonamides – Sulfadimethoxine ,Sulfaethoxypyridazine…

AttractSPETM SCX Milk SCX 40

Caffeine, Acetaminophen, Diclofenac, Ibuprofen,Ketoprofen, Naproxen, Carbamazepine

AttractSPETM W/O - HLBWaste water,

waterHLB 39

Antibacterial Aminoglycosides - - Streptomycin,Dihydrostreptomycin,…

AttractSPETM W/O - HLB Tissue, Milk HLB 39

Antibiotics – Quinolones, Macrolides,Lincosamides, Sulfonamides, Penicillins,Cephalosporine, Pleuromutilins, Diaminopyrimidine derivatives

AttractSPETM W/O - HLB Tissue, Milk HLB 39

NSAID (Non Steroidal Anti inflammatory drug)- Salicylic acid, Phenylbutazone, Flunixin,Tolfenamic acid, Meloxicam, Desoximethasome(IS), Ketoprofen

AttractSPETM W/O - HLB Tissue HLB 39

Penicillin based antibacterials - Ampicillin,Amoxicillin…


Glucocorticoids - Cortisone, Corticosterone,Aldosterone, Betamethasone, Dexamethasone,Flumethasone, Prednisone, Prednisolone,Methylprednisolone


Erythromycin and Clindamycin AttractSPETM W/O - HLB Tissue HLB 39

Praziquantel and Tiamulin AttractSPETM W/O - HLB Tissue HLB 39

Cephalexin AttractSPETM W/O - HLB Fish HLB 39

Quinoxaline-2 -carboxylic acid and 3-methyl

quinoxaline-2-carboxylic acidAttractSPETM SAX

Muscle, Liver,

Kidneys SAX 40

Vancomycin AttractSPETM SCX Fish SCX 40

Valnemulin and Tiamulin AttractSPETM W/O - HLB Fish HLB 39

Phenolic compounds AFFINIMIP® SPE Phenolicsbiological

matricesFS103 34


9

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ANALYTE

FAMILY SPE product- ANALYTES SPE product- ANALYTES MATRICES REF PAGE

Aminopyralid, Clopyralid, PicloramAFFINIMIP® SPE Picolinic

Herbicides

Water,

Compost, SoilFS115 30

Glyphosate, AMPAAFFINIMIP® SPE Glyphosate – AMPA

Waters FS113 31

16 common pesticides - Linuron, Iprodione,Desysopropylatrazine, Desethylatrazine,Aldocarb, Simazine, Carbofuran, Metalaxyl,Atrazin, 2, 4-D, Metazachlor, Dicloran,Phenmedipham, Procymidone, Fenitrothion,Vinclozolin

AttractSPETM W/O - HLB Water HLB 39

Triazine Herbicides - Simazine, Cyanazine,Atrazine…


Acetamide Herbicides - Metolachlor andmetabolites, Alachlor…


Fungicides - Carbendazim, Thiabendazole AttractSPETM SCX Fruit Juice SCX 40

Dopamine, Noradrenaline, Adrenaline, ...AFFINIMIP® SPE

Catecholamines

Plasma,

SerumDG100 32

Metanephrine, Normetanephrine and

3-Methoxytyramine, ...

AFFINIMIP® SPE

Metanephrines

Plasma,

SerumDG101 33

Hydroxylated Polycyclic Aromatic

Hydrocarbons - 2-Naphtol, 2-Hydroxyfluorene,

9-Phenanthrol…

AFFINIMIP® SPE PhenolicsContaminated

soilsFS103 34

Polycyclic Aromatic Hydrocarbons (PAH) AttractSPETM W/O - HLB Waste water HLB 39

Guaïacol AFFINIMIP® SPE Phenolics Wines, Water FS103 34

Carnosic acid AFFINIMIP® SPE Phenolics Meat, Water FS103 34

Hydroquinone AFFINIMIP® SPE Phenolics Water FS103 34

Melamine AttractSPETM SCX Milk, food SCX 40

Cyanuric acid AttractSPETM SAX Milk SAX 40

ARTIFICIAL SWEETENERS - Acesulfame,

Aspartame, Cyclamate, Neohespiridine

dihydrochalcone, Saccharin, Sucralose


COCAINE AND MAIN METABOLITES - Cocaine,

benzoylecgonine and ecgonine methyl ester

AttractSPETM W/O - HLBWaste water HLB 39


10

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Formats available on the catalog for SPE applications

Open Cartridge Reversible Cartridge

LRC Cartridge Cartridge for automate

On-line SPE cartridge 96 Well-plates

Format: 0,7mL ; 2 mLMaterial: PolypropyleneFrits: Polyethylene

Luer compatible

Format: 1 mL; 3 mL; 6 mL; 15 mL; 20mL; 60mL… Material: Polypropylene ; glass (6mL)Frits: Polyethylene ; PTFE (glass cartridges); Glass fiber (Glass cartridges)

Luer compatible

Luer compatible

Format: 10mLMaterial: PolypropyleneFrits: Polyethylene

Cartridge for Multipurpose Sampler (GERSTEL)Format: 1 mL; 3 mL; 6 mLMaterial: PolypropyleneFrits: Polyethylene

Luer compatible

Cartridge for ASPEC (GILSON)Compatible with Open cartridges

FORMATS AVAILABLE FOR SPE APPLICATIONS

For other formats, please contact us

I.D. 2,1 and 4,6mmLength: 20mm

11

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Food / Feed Safety

Environment

Cosmetics

Pharmaceutical R&D

AFFINIMIP® SPESelective Solid Phase ExtractionMolecularly Imprinted Polymers for the Selective

Extraction of Trace Analytes from Complex Matrices

13

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New Extraction Phase Based on Molecularly Imprinted Polymers

Molecularly imprinted polymer is a polymer with a «memory» of the shape and functional

groups of a template molecule. AFFINISEP designs this material in order to recognize

selectively the target molecule, even in the presence of compounds with structure and

functionality similar to those of the template. Using an imprinting process, we design a

material with high affinity for a target molecule:

AFFINIMIP® SPE - CONCEPTS

Advantages of SPE based molecularly imprinted polymers

Molecular Recognition materialsAFFINISEP designs these materialsfor an broad variety of molecules,such as natural products(mycotoxines, sugars…) ,pharmaceuticals, peptides etc…

High affinity and selectivity

Ensure analysis Reproducibilityand reliability via robustand rapid methodology

AFFINIMIPSynthetic materials

Analytical and preparativeapplications in research andproduction

Stable to•pH variation•Temperature variation•Organic solvents

Selective Solid Phase Extraction

Molecularly Imprinted Polymers for theSelective Extraction of Trace Analytes fromComplex Matrices

14

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AFFINIMIP® SPE is a selective solid phase

extraction based on Molecularly Imprinted

Polymers (MIP) that combines the

advantages of immune-affinity columns

regarding the selectivity and those of a

classic Solid Phase Extraction (SPE) in terms

of costs and robustness.

AFFINIMIP® SPE Selective Extraction

Cartridges

Perfect clean-up system for trace analysis

Thanks to selectivity of AFFINIMIP®SPE,

stringent washing steps can be applied in

order to remove all interferences and thus

minimize matrix effects. It also reduces

ion-suppression effects.

Minimal to no method development

required

A protocol based on three steps (loading,

washing and elution) is supplied with

AFFINIMIP®SPE kits for tested matrices.

No extra-equipment than the usual

required for SPE experiments is necessary.

AFFINIMIP® SPE FOR ANALYTICAL PURPOSE

AFFINIMIP® SPE protocols are as well

defined by 3 steps of loading, washing and

elution. All steps have been already

developed in detail by AFFINISEP and an

instruction sheet is supplied with the

product.

The advantages of AFFINIMIP® SPE are

essential in trace analysis from a complex

matrice in food safety, environment

and pharmaceutical analysis such as

Mycotoxins (Patulin, Ochratoxin A,

Zearalenone), Phenolics, Endocrines

Disruptors (Estrogens, Bisphenol A), Drug

residues (Amphetamines, Zeranol residues)

The SPE protocol is supplied in an

instruction sheet for various complex

matrices.

For other matrices, please contact our

technical support to help you with your

application.

15

Example of an instruction sheet supplied with AFFINIMIP® SPE

www.affinisep.com

Mycotoxins analyses

Mycotoxins are toxic secondary metabolites produced by different fungi present in

agricultural commodities. They are regulated in food and feed due to nephrotoxic,

neurotoxic, carcinogenic, estrogenics, and immunosuppressive effects.

AFFINISEP has developed two sets of product for mycotoxins analyses:

For single mycotoxin extraction

Designed for the analysis of one specific mycotoxin or for mycotoxins analyzed by

fluorescence detection

AFFINISEP has developed the following products

AFFINIMIP® SPE Patulin

AFFINIMIP® SPE Ochratoxin A

AFFINIMIP® SPE Zearalenone

AFFINIMIP® SPE Fumonisins

AFFINIMIP® SPE Deoxynivalenol

For multimycotoxins extraction

Designed for the simultaneous extraction of several mycotoxins which are present in a

same matrix prior to LC-MS/MS analyses

These mycotoxins are all present in the same matrix to be analyzed. Their extraction is done

all at once by SPE. Then the elution solution containing all these mycotoxins is evaporated,

reconstituted and analyzed by LC-MS/MS.

The protocol is short and efficient

AFFINISEP has developed the following products

AFFINIMIP® SPE FumoZON for the analyses of

Fumonisins

Zearalenone

AFFINIMIP® SPE Multimyco LCMSMS for the analyses of

Fumonisins

Aflatoxins

Ochratoxin A

T-2 and HT-2

Zearalenone

Deoxynivalenol

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AFFINIMIP® SPE MultimycoLCMSMS

• SIMULTANEOUS Extraction

• All at once extraction of

- Fumonisins,

- Aflatoxins,

- Ochratoxin A,

- T-2 and HT-2

- Zearalenone (ZON)

- Deoxynivalenol (DON)

• Developed for analysis by LC-MS/MS

• Ready for use and optimized extraction methodology adapted to

cereal matrices

• Fast and very simple protocol

AFFINIMIP® SPE Multimyco LCMSMS are Multimycotoxins solid-phase extraction cartridges that

selectively and SIMULTANEOUSLY clean-up and concentrate Fumonisins, Aflatoxins, Ochratoxin A, T-

2, HT-2, Zearalenone and Deoxynivalenol prior to analysis by LC-MS/MS from complex matrices such

as cereals. In Europe, these mycotoxins are all regulated and they are also present in the same

matrices.

FS118

Ordering information: Catalog number: FS118-02 for 25 cartridges, 3mL

FS118-03 for 50 cartridges, 3mLFS118-04 for 100 cartridges, 3mLFS118-02B for 25 cartridges, 6mLFS118-03B for 50 cartridges, 6mL

Recovery of multimycotoxins analyzed after

AFFINIMIP® SPE Multimyco LCMSMS cleanup

Product Information

Tested matrices : Cereals

Protocol :

Loading : Acetonitrile - Water

Washing : Acetonitrile - Water - AA

Elution : Methanol – Acetonitrile - Formic

acid

Detection : HPLC-MS/MS

Format : 3mL cartridges

Particle diameter range : 25-80 µm

Shipping & Storage : Ambient temperature

For other matrices or formats, please contact

us for technical support

Recovery yield

17

Compound name C° µg/kg R%

Aflatoxin B1 0,4 80

Fumonisin B1 4 75

HT-2 4 97

T-2 4 96

Zearalenone 10 95

Ochratoxin A 4 83

Deoxynivalenol 10600 82

CEREALS

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AFFINIMIP® SPE FumoZON

•SIMULTANEOUS Selective Extraction of Fumonisins B1+B2 and

Zearalenone (ZON) for analysis by LCMS

•Ready for use and optimized extraction methodology adapted to

maize matrices

•Strong reduction of Ion suppression

•Very simple protocol and high capacity cartridges

AFFINIMIP® SPE FumoZON are selective solid-phase extraction cartridges that selectively and

SIMULTANEOUSLY clean and concentrate Fumonisins and Zearalenone prior to analysis by HPLC from

complex matrices such as maize and cereal-based baby food. In Europe, Regulation (EC)

N°1126/2007 sets maximum levels for Zearalenone and Fumonisins in foodstuffs with respectively

20µg/kg and 200µg/kg for maize-based babyfood.

FS109


FS109-03 for 50 cartridges, 3mL

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Zearalenone

Fumonisin B1

Fumonisin B2

Chromatograms obtained after AFFINIMIP® SPEFumoZON Clean-up of a maize flour spiked at38µg/kg with Zearalenone, 2408µg/kg withFumonisin B1 and 630µg/kg with Fumonisin B2.

SampleC°

µg/kgMeanµg/kg

Recoveries %

% RSDR

Zearalenone 20 16.9 84.4 1.6 (n=4)

Fumonisin B1 200 168.6 84.3 1.4 (n=3)

Fumonisin B2 200 185.6 92.81.9

(n=3)

Recovery of Zearalenone, Fumonisins B1 and B2 in

maize-based baby food after AFFINIMIP® SPE

FumoZON cleanup

Product Information

Tested matrices : maize matrices

Capacity: Higher than 10µg/g Zearalenone and

5µg/g Fumonisins B1 and B2

Protocol :

Loading : Methanol – Acetonitrile - Water

Washing : Acetonitrile - Water

Elution : Methanol - Acetic acid

Detection : HPLC-MS ESI






Publications

Application of molecularly imprinted polymers

to determine B1, B2, and B3 fumonisins in

cereal products, M. Bryła, R. Jędrzejczak, M.

Roszko, K. Szymczyk, M. W. Obiedziński, J.

Sękul, M. Rzepkowska, J. Sep. Sci., 36(3), 578-

584, 2013.

Application note - AFFINIMIP® SPE FumoZON -

Maize and Maize-based baby food

MAIZE

Recovery yield

18

www.affinisep.com

0

20000

40000

60000

80000

100000

120000

140000

0 1000 2000 3000 4000 5000 6000 7000

AFFINIMIP®SPE Zearalenone are selective solid-phase extraction cartridges for Zearalenone from

complex matrices such as Maize, Cereal-based Baby Food and Rice.

In Europe, Regulation (EC) N°1881/2006 sets maximum levels for Zearalenone in foodstuffs such as

100µg/kg in cereals and 20µg/kg in maize-based babyfood.

AFFINIMIP® SPE Zearalenone



FS100

Perfect clean-up method for trace

analysis of raw and cooked food

matrices

Fluorescence chromatograms obtained after a

purification with AFFINIMIP® SPE Zearalenone of a cereal

sample (80µg/kg of Zearalenone)

Time (min)

mV

With AFFINIMIP® SPE Zearalenone

No pre-treatment

CEREAL SAMPLE

Zearalenone

Product Information

Tested matrices : Wheat, Maize, Cereal-based

baby food, Edible corn oil and Rice

Capacity: Higher than 10µg/g Zearalenone

Protocol :

Loading : Acetonitrile - Water

Washing : Acetonitrile - Water - Acetic Acid


Detection : HPLC-Fluorescence

(excitation/emission wavelengths of 275 /

450nm)






Publications

Molecularly imprinted polymer solid-phase

extraction for detection of zearalenone in

cereal sample extracts,

Analytica Chimica Acta, 672, 15–19, 2010.

Application note - AFFINIMIP® SPE Zearalenone

- Cereal Products and Baby Food


- Automated Protocol (GILSON GX271

ASPEC™)


– Edible corn oil

High capacity of AFFINIMIP®SPE in

comparison with Immuno Affinity Columns

Comparative study of the capacity between

Immuno-Affinity Columns and AFFINIMIP®SPE

Zearalenone

Zearalenone (ng)

AFFINIMIP®SPE Zearalenone

Immuno-Affinity Columns

Are

a

High capacity - No need of high

dilution

Very simple protocol for complex

matrices

19

www.affinisep.com

AFFINIMIP®SPE Ochratoxin A are selective solid-phase extraction cartridges for Ochratoxin A that

selectively clean and concentrate the toxin prior to analysis by HPLC from matrices such as Wheat,

Maize, Pepper, Paprika, Red and White Wine.

In Europe, Regulation (EC) N°1881/2006 sets maximum levels for Ochratoxin A in foodstuffs such as

5µg/kg in cereals and 2ppb in wine. The regulation (EC) N°105/2010 also defines maximum levels for

spices and liquorice products.

AFFINIMIP® SPE Ochratoxin A

Recovery yield

MatrixC°

Ochratoxin A (µg/kg)

Recoveries %

% RSD

Wheat (n=6) 5 96.3 7.7

Paprika (n=4) 30 93.3 3.4

Grey Pepper (n=3)

30 73.3 5.7

White wine (n=10)

2 91.3 6.2

Red wine (n=4) 2 78.8 2.8

FS101

Time (min)

mV

Fluorescence chromatograms obtained after a

purification with AFFINIMIP® SPE Ochratoxin A of a

wheat sample (2.5µg/kg of Ochratoxin A)

Ochratoxin A

With AFFINIMIP®SPE Ochratoxin A

No pre-treatment

WHEAT

0 2 4 6 8 10 12 14 16 18 20 22 24 26 280

1

2

3

4

5

6

7

8

9

Product Information

Tested matrices : Wheat, Maize, Paprika, red and

white Wine, Pepper

Protocol :

Loading : loading solution

Washing : Acetonitrile - HCl solution


Detection : HPLC-Fluorescence excitation/emission

wavelengths of 333 and 460nm

Format : 3mL and 6mL cartridges



For other matrices or formats, please contact us

for technical support

Publications

Solid-phase extraction using molecularly imprinted

polymers for selective extraction of a

mycotoxin in cereals, Journal of

Chromatography A, 1217, 6668-6673, 2010

Molecularly imprinted polymer-based solid phase

clean-up for analysis of ochratoxin A in ginger

and LC-MS/MS confirmation, Cao J., Zhou S.,

Kong W., Yang M., Wan L., Yang S., Food

control, 33(2), 337-343, 2013.

Application note - AFFINIMIP® SPE Ochratoxin A -

Cereals and Spices

Application note - AFFINIMIP® SPE Ochratoxin A -

Red and White Wine

Other publications and application note on our

website

•Ready for use and optimized extraction protocols adapted for

each tested matrices

•Perfect clean-up system for trace analysis of Ochratoxin A

•Unambiguous and reliable analysis

20

Ordering information:Catalog number: FS101-02 for 25 cartridges, 3mL

FS101-03 for 50 cartridges, 3mLFS101-02B for 25 cartridges, 6mLFS101-03B for 50 cartridges, 6mL

www.affinisep.com

AFFINIMIP® SPE Patulin

AFFINIMIP® SPE Patulin are selective solid-phase extraction cartridges

that selectively clean and concentrate this toxin prior to analysis by HPLC

from complex matrices such as apple juice, compote, apple puree

including based on baby food. The World Health Organization, the FDA

and the European Union recommend a maximum concentration of 50

µg/L in apple juice and 25µg/kg in apple puree.

MatrixC° Patulin (ng/mL)

Recoveries %

% RSD

Apple juice (n=4) 25 86 10



Apple puree(n=3)

20 84 4.5

Baby food applepuree (n=4)

10 81.2 2.1

Ordering information:Catalog number:For apple puree and apple juiceFS102-02 for 25 cartridges, 3mL-100mg sorbentFS102-03 for 50 cartridges, 3mL-100mg sorbentFor all apple products and fruit juice, concentratedfruit juiceFS102-02B-200mg for 25 cartridges, 6mL-200mgFS102-03B-200mg for 50 cartridges, 6mL-200mg

FOR KIT Cartridges + pectinase see on p 30

Recovery yield

FS102m

AU

Time (min)

Patulin

APPLE JUICE

•Unique extraction method available on the market

•Easier and faster than Liquid – Liquid Extraction

•Perfect clean-up system for trace analysis of Patulin

•Ready for use and optimized extraction protocol

•Applicable to several apple derived matrices

•Considerable decrease of 5-Hydroxymethylfurfural (HMF) signalProduct InformationTested matrices: Apple juice (clear & cloudy), Apple

and Multifruit puree, baby food, cider, Alcoholpommeau and manzella, Dried apple (format200mg)

Protocol:Loading: Diluted apple juice or apple pureeafter treatment with pectinase enzyme.Washing: NaHCO3 – Water - Diethyl EtherElution: Ethyl acetate

Detection: HPLC-UVFormat: 3 and 6mL cartridgesParticle diameter range : 25-80 µmShipping & Storage : Ambient temperatureFor other matrices or formats, please check ourapplication notebook PATULIN on our website orcontact us for technical support

PublicationsApplication note - AFFINIMIP® SPE Patulin - Apple Juice

and Apple PureeApplication note - AFFINIMIP® SPE Patulin - Baby FoodApplication note - AFFINIMIP® SPE Patulin - CiderApplication note - AFFINIMIP® SPE Patulin - Multifruit

PureeApplication note - AFFINIMIP® SPE Patulin – Dried appleApplication note - AFFINIMIP® SPE Patulin 6mL -150mg -

apple puree

7 8 9 10 11 12 13 14 15 16 17 18 19 20

-

7 8 9 10 11 12 13 14 15 16 17 18 19 20

-10

-5

0

5

10

15

20

25

30

35

40

45

50

55

LC-UV chromatograms obtained after AFFINIMIP®SPE

Patulin Clean-up of an apple juice spiked at 10µk/kg with

Patulin (blue) and not spiked (Red)

21

www.affinisep.com

AFFINIMIP® SPE Deoxynivalenol

•High recovery yield (>80%)

•Efficient clean-up on cereals

•Ready to use and robust protocols

Ordering information:Catalog number: FS117-02B for 25 cartridges, 6mL

FS117-03B for 50 cartridges, 6mL

FS117

AFFINIMIP® SPE Deoxynivalenol are selective solid-phase extraction cartridges for the extraction of

Deoxynivalenol (DON) on cereals prior to analysis by HPLC from cereals such as Oat, Wheat, Corn. In

Europe, Regulation (EC) N°1126/2007 sets maximum levels for Deoxynivalenol in cereals with, for

instance, respectively 1750µg/kg for unprocessed maize, 750µg/Kg for cereal flours and 200µg/kg for

babyfood.

Product information

Tested matrices : oat, wheat, corn

Protocol :

Loading: Water/Acetonitrile/Formic acid

Washing : Water – NaHCO3 - Diethylether

Elution : Ethyl acetate

Detection : LC-MS / UV






CORN

UV chromatograms of corn spiked with DON(800µg/Kg) (repeated twice) and not spiked (red)after AFFINIMIP®SPE Deoxynivalenol clean-up

RT: 0.00 - 7.96

0 1 2 3 4 5 6 7 Time (min)

500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500

Intensity

Deoxynivalenol

LC-MS chromatograms of corn spiked with DON(800µg/Kg) (repeated twice) and not spiked (red)after AFFINIMIP®SPE Deoxynivalenol clean-up

Deoxynivalenol

RT: 0.00 - 7.98

0 1 2 3 4 5 6 7 Time (min)

-30000 -20000 -10000

0 10000 20000 30000 40000 50000 60000 70000 80000 90000

100000 110000 120000

uAU

Recovery yields >80%

Recovery yields >80%

Recovery of corn spiked with DON (800µg/Kg) afterAFFINIMIP®SPE Deoxynivalenol clean-up

Recoveries %

% RSD

Reproductibility (UV, n=6) 83 2

Reproductibility (MS, n=6) 86 7

Repetability (UV, n=3) 83 2

Repetability (MS, n=3) 83 1

21

www.affinisep.com

AFFINIMIP® SPE Estrogens

•Extraction of a broad family of natural and synthetic estrogens by

direct percolation of diluted plasma or water

•Fast and simple protocol

MRM chromatograms from GC-MS/MS analysis of fortified calves’ plasma samples at 0, 10, 40 and 100 pg.mL-1

with 17α-estradiol, 17β-estradiol and estrone. Chromatograms obtained after a clean-up with AFFINIMIP® SPEEstrogens (Courtesy of Emmanuelle Bichon - LABERCA)

BOVINE PLASMA

Publications

Solid-phase extraction using molecularly imprinted

polymer for selective extraction of natural and

synthetic estrogens from aqueous samples, Paolo

Lucci et al., J.Chrom. A, 1218(30), 4828-4833, 2011.

Quantification of estrogens at ppt levels in bovine

plasma by Molecularly Imprinted Solid Phase

Extraction and GC-MS/MS analysis, Emmanuelle

Bichon et al. (LABERCA) Poster session,

Euroresidues VIIth conference (2012).

High efficiency of semi-preparative Supercritical

Fluid Chromatography with Molecularly Imprinted

Polymer as stationary phase (SFC-MIP). Application

on urinary steroids purification for IRMS analysis,

M. Doué et al., HTSP2 2012.

And other publications on our website

Ordering information:Catalog number: FS104-02A for 25 cartridges, 1mL

FS104-03A for 50 cartridges, 1mLFS104-02 for 25 cartridges, 3mLFS104-03 for 50 cartridges, 3mL

FS104

AFFINIMIP® SPE Estrogens are selective solid-phase extraction cartridges that selectively clean and

concentrate the natural or synthetic estrogens family prior to further analysis from complex matrices

such as Water, Plasma or Serum.

Product Information

Tested matrices : Water and plasma.

Protocol :

Loading : Water or diluted plasma


Elution : Methanol

Detection : LC-MS






23

Blank plasma 10ppt 40ppt 100ppt

17β-Estradiol-d3

419>285

17 α/β-Estradiol416>129

17 α/β-Estradiol416>285

www.affinisep.com

AFFINIMIP® SPE Bisphenol A

Ordering information:Catalog number:FS106-02 for 25 cartridges, 3mL PP cartridgesFS106-03 for 50 cartridges, 3mL PP cartridgesFS106-02B for 25 cartridges, 6mL PP cartridgesFS106-03B for 50 cartridges, 6mL PP cartridgesFS106-02G for 25 cartridges, 6mL glass cartridgesFS106-03G for 50 cartridges, 6mL glass cartridges

FS106

AFFINIMIP® SPE Bisphenol A are selective solid-phase extraction cartridges that selectively clean and

concentrate Bisphenol A prior to analysis by HPLC from complex matrices such as River Water or

Babymilk. It is known as an endocrine disruptor and has been banned in plastic infant feeding bottles

in several countries (Canada, EU (directive 2011/8/EU)).

•Perfect clean-up system suitable for all chromatography

techniques: LC, GC, MS...

•Easy quantification of Bisphenol A with fluorescence detection :

no derivatization as needed in GC

•2 grades: glass and polypropylene cartridges

LIQUID INFANT FORMULA

Time (min)Minutes

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 175 6 7 8 9 10 11 12 13 14 15 16 170 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

0

100

200

300

400

500

600

700

800

900

1000

1100

Minutes

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17Minutes

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 175 6 7 8 9 10 11 12 13 14 15 16 170 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

0

100

200

300

400

500

600

700

800

900

1000

1100

Injection of 50µL of infant formula before clean-up

Bisphenol A after clean-up of 15mL of infant formula

Fluorescence chromatograms of infantil formula

spiked with 1µg/L Bisphenol A before (Red) and after

purification (Blue) with AFFINIMIP® SPE Bisphenol A.

24

Product Information

Tested matrices : Water, milk (infant formula),

powdered infant formula or canned food,

vegetable puree for infant, Beer…

Protocol:

Loading: Matrix

Washing: Water - Acetonitrile

Elution: Methanol

Format:

Polypropylene cartridges 3mL and 6mL

Glass cartridges 3mL with Teflon frits: max.

quantity BPA < 5ng/cartridge



For other matrices or formats, please check ourapplication notebook BISPHENOL A on our websiteor contact us for technical support

PublicationsApplication note - AFFINIMIP® SPE Bisphenol A - Beer

Application note - AFFINIMIP® SPE Bisphenol A - infant

formula

Application note - AFFINIMIP® SPE Bisphenol A -

Powdered infant formula

Application note - AFFINIMIP® SPE Bisphenol A – Canned

food

Application note - AFFINIMIP® SPE Bisphenol A –

Vegetable puree in plastic food packaging

www.affinisep.com

AFFINIMIP®SPE Amphetamines

•Extraction of Amphetamine and Methamphetamine derivatives by

direct percolation of diluted serum or urine

•High capacity cartridge for reliable results at high concentrations

Recoveries >85% for each analyte

•Reduction of sample preparation time

DG102

AFFINIMIP® SPE Amphetamines are selective solid phase extraction cartridges that selectively clean

and concentrate Amphetamine and Metamphetamine derivatives from complex matrices such as

Serum or Urine.

Amphetamine has a high possibility of severe psychological and physiological dependence.

As a stupefiant, Amphetamines consumption is prohibited in main european countries. French

regulation has set up an analytical threshold of 50ng/mL in blood for drivers.

Ordering information:Catalog number: DG102-02 for 25 cartridges, 3mL

DG102-03 for 50 cartridges, 3mL

Time (min)

Mass chromatogram (SIM) of 0.5mL of serum spikedwith 100ng/mL of each Amphetamine andMethamphetamine derivatives after clean-up withAFFINIMIP® SPE AmphetaminesAmphetamine (m/z=136); Methamphetamine(m/z=150); MDA: 3,4-Methylenedioxyamphetamine(m/z=180); MDMA, 3,4-Methylenedioxymethamphetamine (m/z=194);MDEA, 3,4-Methylenedioxy-N-ethylamphetamine(m/z=208).

Analyte Recoveries % % RSD

Amphetamine 91.0 5.1

Methamphetamine 90.7 1.9

MDA 92.0 3.7

MDMA 92.2 2.5

MDEA 98.2 5.0

Recovery yield

Recovery yield and %RSD for serum sample spikedwith 100 ng/mL (n=4)

2 4 6 8 10 12 14 16 18 20 22 240

10

20

30

40

50

60

70

80

90

Amphetamine

Methamphetamine

MDA

MDMA

MDEA

SERUM

Product Information

Tested matrices : Urine and serum

Protocol :

Conditioning : Acetonitrile and Deionized

Water Loading : Diluted urine or serum

Washing : W1: Deionized Water

W2: Acetonitrile -

Deionized Water

Elution : Methanol - Formic acid

Detection : HPLC-MS ESI positive mode






Publications

Application note - AFFINIMIP® SPE

Amphetamines - Human urine and serum

25

www.affinisep.com

AFFINIMIP®SPE Chloramphenicol

•Very simple and fast sample preparation

•Direct percolation of honey diluted in water

•High loading capacity: Up to 10g of honey analysed

•Perfect clean-up system for trace analysis of Chloramphenicol

•Detection of 0.3µg/kg with a single quatrupole Mass spectrometer

FS110

AFFINIMIP® SPE Chloramphenicol are selective solid phase extraction

cartridges that selectively clean and concentrate Chloramphenicol from

complex matrices such as Honey.

Chloramphenicol is a broad-spectrum antibiotic widely used in the world in

the past. Several health problems are related by its use. As a consequence,

several countries (e.g. U.S.A, E.U, Canada…) have prohibited its use for food-

producing animals. As no permited limit has been established, E. U has

defined a minimum required performance limits (MRPLs) of 0.3µg/kg for

products of animal origin (Commission decision 2003/181/EC).

However, food matrices are very complexes and induce ion-suppression

phenomena which distort analysis results. AFFINIMIP® SPE Chloramphenicol

solves this problem.


FS110-03A for 50 cartridges, 1mL

2 3 4 5 6 7 8 9 10

1000

2000

3000

4000

5000

6000

7000

8000

9000

10000

2 3 4 5 6 7 8 9 10

1000

2000

3000

4000

5000

6000

7000

8000

9000

10000

MS chromatogram (SIM) of several honeys spiked

with 15.7 µg/kg of Chloramphenicol after clean-up

with AFFINIMIP® SPE Chloramphenicol

Blue traces are not spiked samples

Recovery yield > 90%

Time (min)

inte

nsi

ty

Product Information

Tested matrices : Honey, Bovine Urine

Protocol:

Loading: Diluted honey

Washing: ACN – Water – NH3 – Diethyl

ether

Elution: Methanol

Format: Polypropylene cartridges 1mL





Publications


Chloramphenicol – Honey


Chloramphenicol – Bovine Urine

Application note - AFFINIMIP® SPEChloramphenicol – Shrimp

HONEY

26

www.affinisep.com

AFFINIMIP®SPE Tetracyclines


•Ready for use and optimized protocols

•Perfect clean-up system for trace analysis of Tetracycline,

Oxytetracycline and Chlortetracycline

•LLC-UV or LC-MS/MS

FS112

AFFINIMIP® SPE Tetracyclines are selective solid phase extraction cartridges that selectively clean

and concentrate Chloramphenicol from complex matrices such as Milk.

Tetracyclines (TCs) like oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) are broad-

spectrum antibiotics and are widely used as veterinary medicines and feed additives. These residues

can cause toxic or allergic reactions in hypersensitive individuals and also transfer drug-resistant

bacteria from food to humans.

In response to these concerns and to prevent harmful effects of residual antibiotics in milk on the

human health, various international health organizations have established the maximum residual

limit (MRL) of TCs in all circulating milk in their countries. Worldwide maximum residue levels (MRL)

for tetracycline antibiotics are 100ppb (µg/L).

For such low concentrations, a clean up step is crucial in order to improve the sensitivity, the

reliability and the specificity before LC analysis.


FS112-03A for 50 cartridges, 1mL

Product Information

Tested matrices : Milk

Protocol:

Loading: Milk

Washing: ACN – Water

Elution: Methanol – Formic acid

Format: Polypropylene cartridges 1mL





Publications

Application note - AFFINIMIP® SPE Tetracyclines

– Milk

MILK

27

UV Chromatograms (355nm) obtained after clean-up with AFFINIMIP® SPE Tetracyclines of 1.5mL ofMilk spiked with Tetracyclines at 50µg/L (blue) ornot spiked (red) or of 1.5mL of water spiked withTetracyclines at 50µg/L (pink)

Minutes

0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0

mA

U

0

10

20

30

40

50

60

TCOTC

CTC

Recovery of Tetracyclines spiked at 50µg/L afterAFFINIMIP® SPE Tetracyclines clean-up of 1.5mL ofMilk and relative standard deviation calculatedfrom results generated under reproducibilityconditions.

TetracyclinesC°

(µg/L)Mean (µg/L)

Recoveries %

% RSDR

(n=3)

Tetracycline 50 44.3 88.7 9.5

Oxytetracycline

50 53.7 107.3 10.7

Chlortetracycline

50 40.7 81.3 9.6

www.affinisep.com 28

Since January 2000, Tamoxifen (antioestrogenic molecule) has been included

in the list of prohibited substances by the International Olympic Committee.

So, the presence of these compounds in urine is a doping proof. The analysis

of complex samples, like biofluids, requires a sample preparation step prior to

analysis. AFFINIMIP® SPE Tamoxifen is the perfect tool for selectively extract

Tamoxifen and its main metabolite in biofluids, such as urine (clean-up and

pre-concentration of sample at trace levels). The recoveries of Tamoxifen and

its metabolite 4-HydroxyTamoxifen (4-OH Tam) are greater than 80% at 10

ng/mL.

AFFINIMIP® SPE Tamoxifen

Ordering information:Catalog number: PH101-02 for 25 cartridges, 3mL

PH101-03 for 50 cartridges, 3mL

PH101

LC-UV comparison of urine sample spiked with tamoxifen

and 4-OH tamoxifen (its main metabolite) after extraction

through HLB and AFFINIMIP® SPE

URINE

Comparison between HLB and

AFFINIMIP® SPE Tamoxifen

•Perfect clean-up system for trace analysis of Tamoxifen and its

metabolite in urine

•Unambiguous and reliable analysis

Product Information

Tested matrices : Urine.

Protocol :

Loading : Urine

Washing : Water- Methanol


Detection : HPLC-UV



Stability : 3 years


For other matrices or formats, please contact us for

technical support

PublicationsInterest of molecularly imprinted polymers in the fight

against doping. Extraction of tamoxifen and its main

metabolite from urine followed by high-performance

liquid chromatography with UV detection. Journal of

Chromatography A, 1196–1197, 81–88, 2008.

www.affinisep.com

AFFINIMIP® SPE Zeranol Residues



FS105

AFFINIMIP® SPE Zeranol Residues are selective solid-phase extraction cartridges for Zeranol and its

residues from complex matrices such as urine or meat.

Zeranol (or α-Zearalanol) is a potent nonsteroidal growth promoter used extensively in the United

States and Canada to promote rapid growth rates in animals. Nevertheless, its use is banned in

several countries (e.g. European directive 96/22/EC and in China).

These cartridges can extract Zeranol, Zearalenone, β-Zearalanol (Taleranol), α and β-Zearalenol,

Zearalanone and more broadly, Resorcylic acid lactones. The analysis of these compounds in urine

or meat requires a long and tedious pre-treatment sample (several Liquid-Liquid Extractions and

multi SPE steps).

AFFINIMIP®SPE Zeranol Residues provides similar results in an easier manner. These analyses are

sensitive, rapid and reproducible.

CATTLE URINE SPIKED AT 1ng/mL of 6 main Zeranol metabolites

•Extraction of a broad family of Zeranol residues and Resorcylic acid

lactones compounds

•Ready for use and optimized extraction protocols adapted for each

tested matrices

•Very simple and short pre-treatment sample

Product Information

Tested matrices : Urine and plasma.

Protocol :

Loading : loading solution

Washing : Acetonitrile - Water - Acetic

acid


Detection : HPLC-Fluorescence or LC-MS






Publications

New technological tools for isolating and

measuring growth promoting agents in edible

tissues and biological fluids, Emmanuelle

Bichon et al. (LABERCA) Conference, RAFA

2011

Quantification of a mixture (Zeranol, Zearalenone,

β-Zearalanol, α and β-Zearalenol and Zearelenone)

in bovine urine spiked at 1ng/mL. Comparison of a

clean-up made with AFFINIMIP® SPE Zeranol

Residues + NH2 cartridges and with IAC clean-up.

(Courtesy of Emmanuelle Bichon - LABERCA)

29

Taleranol-d4/

Zeranol-d4

Taleranol/

Zeranol

α and β-

Zearalenol

Zearalenone

Zearelenone

www.affinisep.com

AFFINIMIP® SPE Picolinic Herbicides

•Analysis of Picloram, Aminopyralid and Clopyralid

•High recovery yield (>80%)

•Efficient clean-up of Water, Compost and Soil

•Trace analysis by LC-MS/MS



FS115

AFFINIMIP® SPE Picolinic Herbicides are selective solid-phase extraction cartridges for the

extraction of Picolinic acids based herbicides such as Picloram, Aminopyralid and Clopyralid

from water or compost.

Product information

Tested matrices : compost, water

Protocol :

Loading: Water

Washing : Water - Acetonitrile

Elution : Ethyl acetate /TFA

Detection : LC-MS / UV






30

Structure of picolinic acid

Analysis of herbicides in compost

Percolation solution

Elution solution

AFFINIMIP® SPE

Efficient clean-upand enrichment

www.affinisep.com

AFFINIMIP® SPE Glyphosate - AMPA

Glyphosate [(N-phosphonomethyl)glycine] is a non-selective broad-spectrum herbicide that

is extensively used in agriculture. The biodegradation of Glyphosate (GLY) in the

environment leads to aminomethylphosphonic acid (AMPA).

Both molecules are very difficult to analyze due to their high solubility in water and their

insolubility in organic solvents, making liquid extraction difficult.

AFFINIMIP® SPE Glyphosate – AMPA cartridges enable the extraction and analysis of these

compounds.

Glyphosate AMPA

Ordering information:Catalog number: FS113-02 for 25 cartridges

FS113-03 for 50 cartridges

Mean percentage recoveries of FMOC-derivatized Glyphosate and AMPA spikedin Water after AFFINIMIP® SPE Glyphosate- AMPA clean-up and relative standarddeviation (%RSDR) calculated from resultsgenerated under reproducibility conditions(n=4):

Product information

Tested matrices : waters

Protocol :

Loading: Water - Methanol

Washing : Water - Methanol

Elution : HCl solution (100mM)

Detection : LC-MS / Fluo






n=4Glyphosate AMPA

Recoveries %

87,5 90,2

%RSDR 5,8% 3,8%


•Ready for use and optimized protocols

•High recovery yields (>80%)

29

FS113

www.affinisep.com

•A reliable solution for extraction of Catecholamines and its

metabolites

•Good clean-up method

AFFINIMIP® SPE Catecholamines

Free catecholamines

•Dopamine (DA)

•Norepinephrine or Noradrenaline (NA)

•Epinephrine or Adrenaline (A)

LC-UV chromatogram of a serum sample spiked

with Catecholamines at 450nM with (—) and

without (—) AFFINIMIP® SPE Catecholamines

Internal Standard: 4-hydroxy-3-

methoxybenzylamine (HMBA) (Courtesy of B. Claude

and P. Morin, ICOA, Orléans, France)

NA A

NMN

DA HMBA I.S.


DG100-03 for 50 cartridges, 3mLDG100-02A for 25 cartridges, 1mLDG100-03A for 50 cartridges, 1mL

DG100

AnalytesRecoveries

%

Dopamine (DA) 67

Noradrenaline (NA) 80

Adrenaline (A) 84

3-Methoxytyramine 102

Normetanephrine (NMN) 106

Recovery yield

AFFINIMIP® SPE Catecholamines are selective solid-phase extraction cartridges that selectively clean

and concentrate the Catecholamines and its amino metabolites from complex matrices such as

Plasma or Serum.

Metabolites

•Metanephrine

•Normetanephrine (NMN)

•3-Methoxytyramine

Product Information

Tested matrices : Plasma and serum

Protocol :

Loading : diluted serum or plasma



Detection : HPLC-UV Format: 3mL cartridges





Publications

Analysis of urinary neurotransmitters by

capillary electrophoresis: Sensitivity

enhancement using field-amplified sample

injection and molecular imprinted polymer

solid phase extraction, Bérengère Claude et al.,

Analytica Chimica Acta, 699 (2), 242–248, 2011.

SERUM

32

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AFFINIMIP®SPE Metanephrines

•Extraction of Metanephrines by direct percolation of diluted

plasma or serum

Mass chromatogram (SIM+) of plasma spiked with

60nM of Metanephrines after clean-up with

AFFINIMIP® SPE Metanephrines

MN: Metanephrine (m/z=180); NMN:

Normetanephrine (m/z=166); 3MT: 3-

Methoxytyramine (m/z=151) (Courtesy of B. Claude

and P. Morin, ICOA, Orléans, France)

Metanephrine (MN) Normetanephrine (NMN) 3-methoxytyramine (3MT)


DG101-03 for 50 cartridges, 3mLDG101-02A for 25 cartridges, 1mLDG101-03A for 50 cartridges, 1mL

DG101

NMN

Inte

nsi

ty

MN

3MT

AFFINIMIP® SPE Metanephrines are selective solid phase extraction cartridges that selectively clean

and concentrate Metanephrines from complex matrices such as Plasma or Serum.

Quantification of free metanephrines in Plasma (Metanephrine, Normetanephrine and 3-

methoxytyramine) is considered to be a highly sensitive test for the diagnosis of Pheochromocytoma

and the follow-up of patients. Free Metanephrines are in a very low concentration in this complex

matrix which makes a reliable determination of the compound an analytical challenge.

Time (min)

Product Information

Tested matrices : Plasma and serum

Protocol :

Loading : diluted serum or plasma



Detection : HPLC-UV

Format: 3mL cartridges





Publications


Metanephrine in plasma - comparison with

WCX

SERUM

33

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AFFINIMIP® SPE Phenolics

•Extraction of a broad range of Phenolic compounds

•Reduction of matrix effects and of most interferents

•Several protocols proposed to purify your analyte

Example of extraction of Phenolic compounds from complex matrix

Before MIP clean-upElution after loading of 10mL of dilutedcomplex matrix spiked with:150ng/mL of analyte

100ng/mL of analyte



FS103

Phenolic compound

Time (min)

mV

AFFINIMIP® SPE Phenolics are selective solid-phase extraction cartridges

that selectively clean and concentrate a broad range of phenolic

compounds prior to further analysis.

Phenolic compounds are an important family of products found as natural

substances in plants and life sciences or as synthetic products such as drugs.

Phenolics include a large group of several hundred chemical compounds,

characterized by having at least one aromatic ring with one hydroxyl group

attached.

HPLC profile with a fluorescence detection

CLASS SPECIFIC

Product Information

Protocol :

Protocol with an organic loading solution

Loading : Acetonitrile

Washing : Acetonitrile - Methanol

Elution : Methanol - Acetic Acid

Protocol with an hydro-organic loading

solution Loading : Acetonitrile-Water


Elution : Methanol






34

Example of phenolic moleculeswhich can be purifiedParabensTocopherolsNitrophenolsChlorophenolsCatechinsQuercetinsEtc…

Publications

Molecularly imprinted polymers-liquid

chromatography/fluorescence for the selective

clean-up of hydroxylated polycyclic aromatic

hydrocarbons in soils, O. Baltrons, M. Lopez-

Mesas, Cristina Palet, Franck Lederf and

Florence Portet-Koltalo, Anal. Methods, 2013,

Accepted Manuscript

Application note – AFFINIMIP®SPE Phenolics –

Guaïacol in wine


Parabens in cosmetic products


Carnosic acid in meat

www.affinisep.com

AFFINIMIP® SPE PRODUCT LIST FOR MYCOTOXINS ANALYSES

35

Products Designation Definition ReferenceNber of

cartridges

MultimycotoxinsAFFINIMIP® SPE

MultimycoLCMSMS

3mL Selective SPE cartridges forMultimycotoxin analyses

FS118-02 25

FS118-03 50

6mL Selective SPE cartridges forMultimycotoxin analyses

FS118-02B 25

FS118-03B 50

Zearalenone and Fumonisins

AFFINIMIP® SPEFumoZON

3mL Selective SPE cartridges for Zearalenone and Fumonisins

FS109-02 25

FS109-03 50

Patulin

AFFINIMIP® SPEPatulin

3mL – 100mg Selective SPE cartridgesfor Patulin

FS102-02 25

FS102-03 50

6mL – 200mg Selective SPE cartridgesfor Patulin

FS102-02B-200mg 25

FS102-03B-200mg 50

AFFINIMIP® SPEPatulin & Pectinase

kit

Kit of 3mL selective SPE cartridges forPatulin + 50mL Pectinase enzymesolution

FS102-02K 25

FS102-03K 50

Kit of 6mL - 200mg selective SPEcartridges for Patulin in dried apple +50mL Pectinase enzyme solution

FS102-02KB-200mg

25

FS102-03KB-200mg

50

Ochratoxin AAFFINIMIP® SPE

Ochratoxin A

3mL Selective SPE cartridges forOchratoxin A

FS101-02 25

FS101-03 50

6mL Selective SPE cartridges forOchratoxin A

FS101-02B 25

FS101-03B 50

DeoxynivalenolAFFINIMIP® SPEDeoxynivalenol

6mL -100mg selective SPE cartridges for Deoxynivalenol mycotoxin in food and babyfood

FS117-02B 25

FS117-03B 50

6mL – 200mg selective SPE cartridgesfor Deoxynivalenol mycotoxin in feed

FS117-02B-200mg 25

FS117-03B-200mg 50

ZearalenoneAFFINIMIP® SPE

Zearalenone3mL Selective SPE cartridges for ZON

FS100-02 25

FS100-03 50

Pectinase 50 mL Pectinase enzyme solution REA-001-50mL

For other formats, please contact usAFFINISEP can provide you with other formats than the one described in the product list. Other

formats available on demand and with an adapted protocol can be :

• different volumes of SPE cartridges (1mL, 3mL, LRC, 60mL, etc… )

• 96 well plates,

• HPLC columns,

• Preparative HPLC columns

• the format adapted to your application and your automate

www.affinisep.com

AFFINIMIP® SPE PRODUCT LIST (MISCELLANEOUS)

Products Designation Definition ReferenceNber of

cartridges

Bisphenol AAFFINIMIP® SPE

Bisphenol A

3mL Selective SPE cartridges for BisphenolA (PP)

FS106-02 25

FS106-03 50

6mL Selective SPE cartridges for BisphenolA (PP)

FS106-02B 25

FS106-03B 50

6mL Selective SPE cartridges for BisphenolA (Glass)

FS106-02G 25

FS106-03G 50

EstrogensAFFINIMIP® SPE

Estrogens

1mL Selective SPE cartridges for EstrogensFS104-02A 25

FS104-03A 50

3mL Selective SPE cartridges for EstrogensFS104-02 25

FS104-03 50

CatecholaminesAFFINIMIP® SPECatecholamines

3mL Selective SPE cartridges forCatecholamines

DG100-02 25

DG100-03 50

1mL Selective SPE cartridges forCatecholamines

DG100-02A 25

DG100-03A 50

MetanephrinesAFFINIMIP® SPEMetanephrines

3mL Selective SPE cartridges forMetanephrines

DG101-02 25

DG101-03 50

1mL Selective SPE cartridges forMetanephrines

DG101-02A 25

DG101-03A 50

Picloram, Aminopyralid,

Clopyralid

AFFINIMIP® SPEPicolinic

Herbicides

3mL Selective SPE cartridges for Picolinicacid based herbicides

FS115-02 25

FS115-03 50

Glyphosate, AMPA

AFFINIMIP® SPEGlyphosate -

AMPA

3mL Selective SPE cartridges forGlyphosate and AMPA

FS113-02 25

FS113-03 50

AmphetaminesAFFINIMIP® SPEAmphetamines

3mL Selective SPE cartridges forAmphetamines derivatives

DG102-02 25

DG102-03 50

ChloramphenicolAFFINIMIP® SPE

Chloramphenicol1mL Selective SPE cartridges forChloramphenicol

FS110-02A 25

FS110-03A 50

TamoxifenAFFINIMIP® SPE

Tamoxifen3mL Selective SPE cartridges for Tamoxifen PH101-02 25

TetracyclinesAFFINIMIP® SPE

Tetracyclines1mL Selective SPE cartridges for Tetracyclines

FS112-02A 25

FS112-03A 50

Zeranol ResiduesAFFINIMIP® SPE

Zeranol Residues3mL Selective SPE cartridges for ZeranolResidues

FS105-02 25

FS105-03 50

PhenolicsAFFINIMIP® SPE

Phenolics3mL Selective SPE cartridges for Phenoliccompounds

FS103-03 50

34

www.affinisep.com

AttractSPETM

POLYMERIC-BASED PRODUCTS FOR SPE

37


The choice of the suitable AttractSPETM columns can be done by using the followingmethod:- Check if a method already exists on the application notebook of our website-Determination of the nature of the analyte (neutral, acid, base)-Determination of the pKa-Choice of the AttractSPETM columns by using the following chart and application of thegeneral protocol described on the instruction sheet- Analysis of the recovery yields

Sample preparation

Conditioning and equilibration

loading of the solution

Washing with Formic acid 2%

2mL Methanol

2mL 5% NH4OH /Methanol

Sample preparation



Washing with NH4OH 5%

2mL Methanol

2mL 5% Formic acid /Methanol

Sample preparation



Washing with Methanol/ Water

2mL Methanol

Neutral compounds

Strong basesBasesStrong acids Acids

AttractSPETM

WAXStrong acids

pKa<1

AttractSPETM

SCXBases

pKa 2-10

AttractSPETM

WCXStrong Bases

pKa >10

AttractSPETM

SAXAcids pKa 2-8

AttractSPETM

DVBNeutral

hydrophobic

AttractSPETM

W/O - HLBNeutral

AttractSPETM are based on polymeric sorbents dedicated to sample clean-up for the

extraction of compounds from complex matrices. These cartridges contain polymeric

sorbents which support the six common retention mechanisms used in today’s analytical

laboratory:

•Hydrophilic Lipophilic Balance (AttractSPETM W/O - HLB)

•Mixed-mode Strong Cation Exchange (AttractSPETM SCX)

•Mixed-mode Weak Cation Exchange (AttractSPETM WCX)

•Mixed-mode Strong Anion Exchange (AttractSPETM SAX)

•Mixed-mode Weak Anion Exchange (AttractSPETM WAX)

•Reversed phase (AttractSPETM DVB)

AttractSPETM cartridges provide the advantages of robustness, simplified method

development, wide applicability and are not affected by drying out. The combination of the

water-wettable optimised surface chemistry, high surface area and pH stability ensures high

reproducible recoveries for a wide range of analytes.

POLYMERIC AttractSPETM CARTRIDGES

www.affinisep.com

AttractSPETM W/O - HLB is an uncharged Hydrophilic and Lipophilic sorbent interacting with both, hydrophilic and hydrophobic interactions.

AttractSPETM W/O - HLB particularly suits for

the extraction of a wide range of analytes

(polar, apolar, neutral, acid, basic…)

Product Information

Protocol :

Loading : Pre-treated sampleWashing: Methanol - WaterElution: Methanol

Format : see product list

Particle diameter range : 40 µm

Pore size: 70 ÅSurface area: 800 m2/g



Reversed phase SPE for extraction of

hydrophobic analytes

AttractSPETM DVB is a polystyrene-

divinylbenzene copolymer presenting a high

hydrophobicity used as a reversed-phase.

AttractSPETM DVB particularly suits for the

extraction of hydrophobic analytes.

Product Information



Pore size: 60 ÅSurface area: 600 m2/g


39

AttractSPETM W/O - HLB

AttractSPETM DVB

Mixed-mode SPE for extraction of strong acid

analytes

AttractSPETM WAX is a weak anion exchange

sorbent interacting with the analytes via a

mixed mode retention mechanism, ion

exchange with weak basic functional groups

and reversed phase.

AttractSPETM WAX particularly suits for the

extraction of strong acids.

Product Information

Protocol :

Loading : Neutral to Acidified sampleWashing 1: Formic acid – WaterWashing 2 or Elution 1: Methanol Elution 2: Ammonium hydroxide – Methanol


Pore size: 60 ÅSurface area: 650 m2/gIonic capacity: 0.5 meq/g



AttractSPETM WAX

www.affinisep.com

Mixed-mode SPE for extraction of strong basic

analytes

AttractSPETM WCX is a weak cation exchange



exchange with weak acid functional groups and

reversed phase.

AttractSPETM WCX particularly suits for the

extraction of strong bases and quaternary

amines.


Product Information

Protocol :

Loading: Neutral to Basic sampleWashing 1: Ammonium hydroxide - WaterWashing 2: MethanolElution: Formic acid - Methanol


Pore size: 70 ÅSurface area: 850 m2/gIonic capacity: 0.77meq/g



40

AttractSPETM WCX

Mixed-mode SPE for extraction of weak acid

analytes

AttractSPETM SAX is a strong anion exchange



exchange with strong basic functional groups

and reversed phase.

AttractSPETM SAX particularly suits for the

extraction of weak acids.

Product Information

Protocol :

Loading: Neutral to Basic sampleWashing 1: Ammonium hydroxide - WaterWashing 2: MethanolElution: Formic acid - Methanol



Pore size: 60 ÅSurface area: 600 m2/gIonic capacity: 0.3 meq/g


AttractSPETM SAX

Mixed-mode SPE for extraction of weak basic

analytes

AttractSPETM SCX is a strong cation exchange



exchange with strong acid functional groups

and reversed phase.

AttractSPETM SCX particularly suits for the

extraction of weak bases.

Product Information

Protocol :

Loading : Neutral to Acidified sampleWashing 1: Formic acid - WaterWashing 2 or Elution 1: Methanol Elution 2: Ammonium hydroxide – Methanol


Pore size: 60 ÅSurface area: 600 m2/gIonic capacity: 1meq/g



AttractSPETM SCX

www.affinisep.com




• 96 well plates,

• HPLC columns,



POLYMERIC AttractSPETM PRODUCT LIST

41

Cartridgesformat,Sorbentamount

Nber /boxAttractSPETM

W/O - HLBAttractSPETM

SCXAttractSPETM

WCXAttractSPETM

SAXAttractSPETM

WAXAttractSPETM

DVB

1mL, 30mg 100 HLB-100.S.1.30 SCX-100.S.1.30 WCX-100.S.1.30 SAX-100.S.1.30

WAX-100.S.1.30

DVB-100.S.1.30

3mL, 60mg50 HLB-50.S.3.60 SCX-50.S.3.60 WCX-50.S.3.60 SAX-50.S.3.60 WAX-50.S.3.60 DVB-50.S.3.60

100 HLB-100.S.3.60 SCX-100.S.3.60 WCX-100.S.3.60 SAX-100.S.3.60WAX-

100.S.3.60DVB-100.S.3.60

6mL, 200mg


25.S.6.200DVB-25.S.6.200


50.S.6.200DVB-50.S.6.200

100HLB-

100.S.6.200SCX-100.S.6.200

WCX-100.S.6.200

SAX-100.S.6.200

WAX-100.S.6.200

DVB-100.S.6.200

6mL, 500mg


25.S.6.500DVB-25.S.6.500


50.S.6.500DVB-50.S.6.500

100HLB-

100.S.6.500SCX-100.S.6.500

WCX-100.S.6.500

SAX-100.S.6.500

WAX-100.S.6.500

DVB-100.S.6.500

96 wellsPlate, 30mg

1 HLB-1.96W.30 SCX-1.96W.30 WCX-1.96W.30 SAX-1.96W.30 WAX-1.96W.30 DVB-1.96W.30

Reversible 0.7mL, 30mg

25HLB-

25.REV.1.N10SCX-

25.REV.1.N10WCX-

25.REV.1.N10SAX-

25.REV.1.N10WAX-

25.REV.1.N10DVB-

25.REV.1.N10

50HLB-

50.REV.1.N10SCX-

50.REV.1.N10WCX-

50.REV.1.N10SAX-

50.REV.1.N10WAX-

50.REV.1.N10DVB-

50.REV.1.N10

Reversible 0.7mL, 100mg (200mg for DVB)

25 HLB-25.REV.1.F SCX-25.REV.1.F WCX-25.REV.1.F SAX-25.REV.1.FWAX-

25.REV.1.FDVB-

25.REV.1.F

50 HLB-50.REV.1.F SCX-50.REV.1.F WCX-50.REV.1.F SAX-50.REV.1.FWAX-

50.REV.1.FDVB-

50.REV.1.F

Reversible 2mL, 225mg

25HLB-

25.REV.2.N10SCX-

25.REV.2.N10WCX-

25.REV.2.N10SAX-

25.REV.2.N10WAX-

25.REV.2.N10

50HLB-

50.REV.2.N10SCX-

50.REV.2.N10WCX-

50.REV.2.N10SAX-

50.REV.2.N10WAX-

50.REV.2.N10

www.affinisep.com

www.affinisep.com

SilactSPETM

SILICA-BASEDPRODUCTS FOR SPE

43

www.affinisep.com

SilactSPETM Silica-Based Sorbents

SilactSPETM C18 : Strongly hydrophobic and

non-polar sorbent

It was recently developed as an innovative C18

phase characterized by a homogeneous

coverage of the silane on the surface.

SilactSPETM C18 particularly suits for the

extraction of acidic, neutral and basic

compounds from aqueous solutions, various

organic compounds from water, and drugs and

metabolites from physiological fluids.

Product Information

Loading : 17 % C

Endcapping : Yes

Silica type : 60 Å, 500 m2/g, 40-63 µm

SilactSPETM C8: Moderately hydrophobic and

non-polar sorbent

Sorbent C8 is more selective than Sorbent C18

for big compounds such as PAH, vitamin D, and

oils as well as greasy compounds.

SilactSPETM C8 particularly suits for the extraction of extremely non-polar compounds.

Product Information

Loading : 12 % C

Endcapping : Yes


44

SilactSPETM Phenyl : Moderately hydrophobic

and non-polar sorbent

it particularly suits for the extraction of non-

polar compounds with different selectivities

through π-π interactions including aromatic

compounds and other non-polar phases.

Product Information

Loading : 9 % C

Endcapping : Yes


Ordering information: References and formatson the product list

SilactSPETM Silica : Most polar sorbent

It presents a slightly acidic character and is

used to extract various compounds from non-

polar solvents through hydrogen bonding.

Product Information


SilactSPETM Cyano : Moderately polar sorbent

It is used as a normal phase (less polar

compared to silica).

SilactSPETM Cyano particularly suits for the

extraction of acidic, basic and neutral

compounds from aqueous solutions. It is also

used as a reversed-phase (less hydrophobic

than C8 and C18).

Product Information

Loading : 7 % C

Endcapping : Yes


SilactSPETM products are Silica-based sorbents SPE cartridges available in different formats with

different sorbents, and in bed weights up to 10 grams (>10 g are also available in SilactSPE OT

formats)

www.affinisep.com

SilactSPETM Silica-Based Sorbents

45

SilactSPETM Alumina-Acidic, Neutral and Basic:

Alumina can present either cationic, neutral

and acidic character. It is used in a similar

fashion as for the SilactSPE Silica. The

difference is that Alumina is more stable at

high pH than silica.

SilactSPETM Alumina particularly suit for the

retention of aromatic compounds, aliphatic

amines and compounds containing

electronegative functions.

Product Information

Alumina type : 60 Å, 0.9 g/mL, 50-200 µm

Ordering information: References and formatson the product list

SilactSPETM Amine: Weak anion exchanger

silica-based sorbent

SilactSPETM Amino avoids irreversible retention

of acidic molecules (pKa < 3) and particularly

suits for the separation of peptides, drugs and

metabolites from physiological fluids, poly- and

monosaccharides and structural isomers.

Product Information

Loading : 1.6 mmol/g

Endcapping : Yes


SilactSPETM Carbonate: General base quencher

SilactSPE Carbonate is the silica-bound

equivalent of tetramethyl ammonium

carbonate and is used as a general base to

quench a reaction, free base amines in their

ammonium salt form and to scavenge acids,

boronic acids and acidic phenols including

HOBt.

Product Information

SilactSPETM PSA: Weak anion exchanger silica-

based sorbent

Less polar sorbent than SilactSPETM Amineused for its replacement with analytes that aretoo strongly retained on an amine phase.

Product Information

Endcapping : Yes

Silica type : 60 Å, 500 m2/g, 40-63

µm

SilactSPETM Florisil and Florisil PR (MgO3Si) :

Polar sorbent

They present a basic character used to extract

non-polar to moderately polar compounds

from non-polar solvents.

They particularly suit for the retention of

chlorinated pesticides, polychlorinated

biphenyl (PCB’s) and polysaccharides due to

the magnesium ion.

Product Information

Florisil type : 75-150 µm

Florisil PR type : 150-200 µm

www.affinisep.com




• 96 well plates,

• HPLC columns,



46

Non polar sorbents Polar sorbents

Cartridgesformat,Sorbentamount

#/box

SilactSPETM

C18SilactSPETM

C8SilactSPETM

PhenylSilactSPETM

SilicaSilactSPETM

CyanoSilactSPETM

FlorisilSilactSPETM

Florisil PR

1mL, 50mg 100 C18-100.S.1.50 C8-100.S.1.50 Phe-100.S.1.50 Si-100.S.1.50 CN-100.S.1.50 Flo-100.S.1.50

FloPR-100.S.1.50

1mL, 100mg 100C18-

100.S.1.100C8-100.S.1.100

Phe-100.S.1.100

Si-100.S.1.100CN-

100.S.1.100Flo-

100.S.1.100FloPR-

100.S.1.100

3mL, 200mg 50C18-

50.S.3.200C8-

50.S.3.200Phe-

50.S.3.200Si-

50.S.3.200CN-

50.S.3.200Flo-

50.S.3.200FloPR-

50.S.3.200

3mL, 500mg 50C18-

50.S.3.500C8-

50.S.3.500Phe-

50.S.3.500Si-

50.S.3.500CN-

50.S.3.500Flo-

50.S.3.500FloPR-

50.S.3.500

6mL, 500mg50

C18-50.S.6.500

C8-50.S.6.500

Phe-50.S.6.500

Si-50.S.6.500

CN-50.S.6.500

Flo-50.S.6.500

FloPR-50.S.6.500

6mL, 1g 50C18-

50.S.6.1000C8-

50.S.6.1000Phe-

50.S.6.1000Si-

50.S.6.1000CN-

50.S.6.1000Flo-

50.S.6.1000FloPR-

50.S.6.1000

6mL, 2g50

C18-50.S.6.2000

C8-50.S.6.2000

Phe-50.S.6.2000

Si-50.S.6.2000

CN-50.S.6.2000

Flo-50.S.6.2000

FloPR-50.S.6.2000

12mL, 2g 20C18-

20.S.12.2000C8-

20.S.12.2000Phe-

20.S.12.2000Si-

20.S.12.2000CN-

20.S.12.2000Flo-

20.S.12.2000FloPR-

20.S.12.2000


25C18-

25.REV.1.200C8-

25.REV.1.200Phe-

25.REV.1.200Si-

25.REV.1.200CN-

25.REV.1.200Flo-

25.REV.1.200FloPR-

25.REV.1.200


25C18-

25.REV.2.750C8-

25.REV.2.750Phe-

25.REV.2.750Si-

25.REV.2.750CN-

25.REV.2.750Flo-

25.REV.2.750FloPR-

25.REV.2.750

SilactSPETM PRODUCT LIST

www.affinisep.com




• 96 well plates,

• HPLC columns,



47

Polar sorbentsOthers sorbents

Cartridgesformat,Sorbent amount

#/boxSilactSPETM

Alumina Acidic

SilactSPETM

Alumina Neutral

SilactSPETM

Alumina BasicSilactSPETM

AmineSilactSPETM

PSASilactSPETM

Carbonate

1mL, 50mg 100 AluA-100.S.1.50 AluN-100.S.1.50 AluB-100.S.1.50 NH2-100.S.1.50 PSA-100.S.1.50 CO3-100.S.1.50

1mL, 100mg 100AluA-

100.S.1.100AluN-

100.S.1.100AluB-

100.S.1.100NH2-

100.S.1.100PSA-

100.S.1.100CO3-

100.S.1.100

3mL, 200mg 50AluA-

50.S.3.200AluN-

50.S.3.200AluB-

50.S.3.200NH2-

50.S.3.200PSA-

50.S.3.200CO3-

50.S.3.200

3mL, 500mg 50AluA-

50.S.3.500AluN-

50.S.3.500AluB-

50.S.3.500NH2-

50.S.3.500PSA-

50.S.3.500CO3-

50.S.3.500

6mL, 500mg50

AluA-50.S.6.500

AluN-50.S.6.500

AluB-50.S.6.500

NH2-50.S.6.500

PSA-50.S.6.500

CO3-50.S.6.500

6mL, 1g 50AluA-

50.S.6.1000AluN-

50.S.6.1000AluB-

50.S.6.1000NH2-

50.S.6.1000PSA-

50.S.6.1000CO3-

50.S.6.1000

6mL, 2g50

AluA-50.S.6.2000

AluN-50.S.6.2000

AluB-50.S.6.2000

NH2-50.S.6.2000

PSA-50.S.6.2000

CO3-50.S.6.2000

12mL, 2g 20AluA-

20.S.12.2000AluN-

20.S.12.2000AluB-

20.S.12.2000NH2-

20.S.12.2000PSA-

20.S.12.2000CO3-

20.S.12.2000


25AluA-

25.REV.1.200AluN-

25.REV.1.200AluB-

25.REV.1.200NH2-

25.REV.1.200PSA-

25.REV.1.200CO3-

25.REV.1.200


25AluA-

25.REV.2.750AluN-

25.REV.2.750AluB-

25.REV.2.750NH2-

25.REV.2.750PSA-

25.REV.2.750CO3-

25.REV.2.750

SilactSPETM PRODUCT LIST

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On-line SPE

49


Product Product referenceNber

columnI.D. (mm)

Lenght (mm)

On-line AttractSPETM

W/O - HLB columns

OnlineSPE-HLB-1.2.20 1 2.1 20

OnlineSPE-HLB-1.5.20 1 4.6 20

For other on-line SPE products, please contact usAFFINISEP can provide you with on-line SPE of all products on demand

On-line SPE columns for hydrophilic – hydrophobic polymers

ON-LINE SPE PROCEDURE STEPS

SPE technique can be coupled on-line to HPLC for a high sensitivity or for limited amount of

sample. An on-line SPE column containing the SPE sorbents is coupled within the injection

loop of a six-port rotary valve. The on-line SPE column is reusable.

A three steps process is used:

1- Sample injection: The valve is configured with the injector directly in contact with the on-

line SPE column. The sample is injected and goes through the on-line SPE column where the

analytes remain.

2- Washing: a solution is used to wash out most interferences.

3- Analysis: The valve is switched. The analytes are eluted out of the sorbent by the LC

mobile phase and transfered into the analytical column for their analyses.

ON-LINE SPE – DESCRIPTION AND PRODUCT LIST

Sample injection and washing (1 and 2) Sample elution and analysis (step 3)

Analytical column

Detector

HPLC Pump

SPE column

Sample injectorWaste

Analytical column

Detector

HPLC Pump

SPE column

Sample injectorWaste

Flow Flow

Product Product referenceNber

columnI.D. (mm)

Lenght (mm)

On-line AFFINIMIP® PHENOLICS columns

OnlineSPE-PHE-1.2.20 1 2.1 20

OnlineSPE-PHE-1.5.20 1 4.6 20

On-line SPE columns for AFFINIMIP® Phenolics

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QcleanupTM

QuEChERS

51


QuEChERS is a sample pretreatment initially developed by Michelangelo Anastassiades and

Steven Lehotay is mainly used for the analysis of multiple pesticides into fruits and

vegetables. It is the acronym of Quick, Easy, Cheap, Effective, Rugged ans Safe. Three main

methods are currently used the original method, the European standardized method EN

15662 and the AOAC official method 2007.01. Each method required an extraction process

with salts and a clean-up process with SPE cartridges or by dispersive SPE.

QcleanupTM - Description

AFFINISEP supplies all products required to carry out QuEChERS according to AOAC or CENincluding dispersive SPE products or SPE cartridges.


QcleanupTM - PRODUCT LIST

QcleanupTM EXTRACTION SALTS

QuEChERS methods DescriptionPouches /

boxProduct

reference

Original method4g MgSO4

1g NaCl50 EXT.ORL.50

EN 15662

1g Trisodium citrate Dihydrate0.5g Disodium hydrogencitrate

sesquihydrate1g NaCl and 4g MgSO4

50 EXT.EN.50

AOAC 2007.01 1.5g Sodium Acetate and 6g MgSO4 50 EXT.AOAC.50

QcleanupTM DISPERSIVE SPE PRODUCTS

Method Description Nber/box Product reference

For General Fruits & Vegetables

EN 15662150mg MgSO4 + 25mg PSA 100 tubes of 2mL dSPE.EN.GFV.100.2

900mg MgSO4 + 150mg PSA 50 tubes of 15mL dSPE.EN.GFV.50.15

AOAC 2007.01

150mg MgSO4 + 50mg PSA 100 tubes of 2mL dSPE.AOAC.GFV.100.2

1200mg MgSO4 + 400mg PSA 50 tubes of 15mL dSPE.AOAC.GFV.50.15

For Pigmented Fruits & Vegetables

EN 15662150mg MgSO4 + 25mg PSA + 2.5mg CB 100 tubes of 2mL dSPE.EN.PFV.100.2

900mg MgSO4 + 150mg PSA + 15mg CB 50 tubes of 15mL dSPE.EN.PFV.50.15

AOAC 2007.01

150mg MgSO4 + 50mg PSA + 50mg CB 100 tubes of 2mL dSPE.AOAC.PFV.100.2

1200mg MgSO4 + 400mg PSA + 400mg CB 50 tubes of 15mL dSPE.AOAC.PFV.50.15

For Highly Pigmented and Fatty Fruits & Vegetables

EN 15662150mg MgSO4 + 25mg PSA + 7.5mg CB 100 tubes of 2mL dSPE.EN.HPFV.100.2

900mg MgSO4 + 150mg PSA + 45mg CB 50 tubes of 15mL dSPE.EN.HPFV.50.15

AOAC 2007.01

150mg MgSO4 + 50mg PSA + 50mg CB +50mg C18

100 tubes of 2mL dSPE.AOAC.HPFV.100.2

1200mg MgSO4 + 400mg PSA + 400mg CB + 400mg C18

50 tubes of 15mL dSPE.AOAC.HPFV.50.15

For Fatty and waxed Fruits & Vegetables

EN 15662150mg MgSO4 + 25mg PSA + 25mg C18 100 tubes of 2mL dSPE.EN.FWFV.100.2

900mg MgSO4 + 150mg PSA + 150mg C18 50 tubes of 15mL dSPE.EN.FWFV.50.15

AOAC 2007.01

150mg MgSO4 + 50mg PSA + 50mg C18 100 tubes of 2mL dSPE.AOAC.FWFV.100.2

1200mg MgSO4 + 400mg PSA + 400mg C18 50 tubes of 15mL dSPE.AOAC.FWFV.50.15

QcleanupTM products for dispersive SPE are mixtures of powder in 2mL or 15mL centrifugation tubesfor main scenarios encountered during pesticide analyses. This mixture contains magnesium sulfateanhydrous (MgSO4), primary secondary amine (PSA), carbon black (CB) or C18.

QcleanupTM extraction salts are the three main salts mixtures used in QuEChERS method.

www.affinisep.com

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MYCOTOXINS

STANDARDS

55

www.affinisep.com

Mycotoxines ProductConcentration

(µg/mL) Volume Référence

Aflatoxins

Aflatoxin B1 2 1mL REA-AFLB1-1ML

Aflatoxin B2 0.5 1mL REA-AFLB2-1ML

Aflatoxin G1 2 1mL REA-AFLG1-1ML

Aflatoxin G2 0.5 1mL REA-AFLG2-1ML

Mixture Aflatoxins: B1-B2-G1-G2 2-0.5-2-0.5 1mL REA-MIXAFL-1ML

Aflatoxin M1 0.5 1mL REA-AFLM1-1ML

Ochratoxin Ochratoxin A 100 1mL REA-OTA-1ML

Patulin Patulin 100 1mL REA-PAT-1ML

Trichothecenes AT2 Toxin 100 1mL REA-T2-1ML

HT-2 Toxin 100 1mL REA-HT2-1ML

Trichothecenes B Deoxynivalenol (DON) 100 1mL REA-DON-1ML

Zearalenone Zearalenone 100 1mL REA-ZON-1ML

Fumonisines

Fumonisine B1 50 1mL REA-FB1-1ML

Fumonisine B2 50 1mL REA-FB2-1ML

Mixture Fumonisine: B1-B2 50 1mL REA-MIXFB-1ML

MYCOTOXINS STANDARDS

56

AFFINISEP proposes the following Mycotoxins standards for mycotoxins analyses :

Stability: 1 year

Storage: 4°C

Mycotoxins are toxic byproducts of mold infestations which can

contaminate food. To protect consumers from the health risks,

most countries have adopted regulations to limit exposure to

mycotoxins.

Our mycotoxins standards are accurately prepared solutions of

a mycotoxin or a mixture of mycotoxins to a determined

concentration. These standards can be used for the

implementation of the calibration curve as well as for the

development of a new analytical methods. They are supplied

with a certificate of analysis.

MYCOTOXINS STANDARDS – Product List

Please check our website for an updated list of standard solutions

www.affinisep.com

SPE ACCESSORIES

57

www.affinisep.com

Manifold ACC-MAN1 Like all chromatography techniques, Use of SPE cartridges needs

a precise control of flow rate for maintaining reproducible

extractions. Solid Phase extraction Vacuum Manifold allows you

to control the flow and to process up to 12 (12-port version) or

24 (24-port version) AFFINIMIP® SPE samples simultaneously, to

gain significantly time during sample preparation steps.

SPE Adapter &

Reservoir kit

ACC-AR1 Tube adapters serve to pile one SPE tube on top of another to

provide different selectivities. A larger empty syringe barrel can

be stacked on top of a smaller SPE tube to act as a larger load

reservoir. Or, they can serve as an adapter for positive pressure

methods (e.g. from a syringe or air/ N2 line).

Mini-Vap ACC-VAP1 The 6-Port Mini-Vap concentrator/evaporator processes six vials

at one time. The Mini-Vap includes a needle valve for fine

metering of air or nitroge n drying gas.

Mini PUMP ACC-PUMP Mini diaphragm vacuum pump for solid phase extraction

experiments

Portable

5.5L/min

~120 torr vacuum

Oil-free

portable

Vacuum pump trap ACC-TRAP SPE Vacuum pump trap kit

Installed between the manifold and the vacuum pump, it

collects all liquids that are aspirated preventing contamination

of the vacuum pump with a capacity of 1L.

AFFINISEP proposes the complete set of equipments required to carry out SPE experiments:

SPE ACCESSORIES

58

SPE Accessories Designation Definition Reference

Manifold SPE Vaccum Manifold 12-port model ACC-MAN1

SPE Adapter & Reservoir

kitSPE Adapter & Reservoir kit

Kit of 12 reservoirs 60ml and adapters

for use with 1,3 & 6 mL cartridgesACC-AR1

Mini-VapMini

Evaporator/Concentrator

6 port Mini-Vap

Evaporator/Concentrator for use with 1

to 250mL containers

ACC-VAP1

Mini PUMP Mini vacuum pumpLaboport diaphragm vacuum mini

pump, 5.5L/minACC-PUMP

Vacuum pump trap SPE Vacuum pump trap kit 1L trap kit ACC-TRAP

SPE ACCESSORIES – Product list

www.affinisep.com

CUSTOM-MADE

PRODUCTS &

SERVICES

59

www.affinisep.com

AFFINISEP offers full services for the design and synthesis of polymers complying with your

specifications.

With fully integrated technology platforms in organic, polymer and analytical chemistry,

AFFINISEP has been partner in more than 40 projects and helps its customers by innovative

solutions for their complexes challenges.

AFFINISEP has developed a library of monomers giving a family of selective stationary phases

based on its proprietary technology, which have shown a strong potential for the extraction,

purification and detection of various compounds.

Did you know ?Molecularly imprinted polymer (MIP) is a polymer with a «memory» of the shapeand the functional groups of a target molecule. This material is designed in orderto recognize selectively this molecule, even in the presence of compounds withstructure and functionality similar to those of this molecule. High molecularrecognition properties can be achieved with these MIPs for a variety of moleculesand can be useful for its extraction, purification and detection.

60

CUSTOM-MADE PRODUCTS & SERVICES

Why trusting AFFINISEP? AFFINISEP supplies

• The most comprehensive bank of sorbents

•From Silica to Polymers

•From conventional to very selective

•Reversed-phase, ionic exchange, MIPs etc…

•Our Expertise on MIP, sample preparation, SPE protocol and detection

kit development

•Quick and efficient development

•Reactivity

Custom-made

solutions for:

SPE for analytical

applications

Purifications SPE for

radiopharma

Molecularly imprinted polymers

development

Custom-made

formats

www.affinisep.com

If you need the development of new polymer for your application, please send us an email

to [email protected] or describe your needs using

http://www.polyintell.com/services/request-service-online/. You can describe your

application and our scientists will shortly evaluate your queries before contacting you as

soon as possible.

61

STEP 1

STEP 2

STEP 3

Procedure for custom-made polymer phase

CustomerFe

edb

ack

and

op

tim

isat

ion

•Screening

• Elaboration ofapplication protocol• Delivery ofsamples/ prototypesto customer forvalidation

• Scale-up• QC report

Bulk material, SPE cartridges or HPLCcolumns

Step 1: We offer you a screening of our library

which consists of several hundreds AFFINISEP

phases to find the suitable one for your

separation problem. The knowledge of the

structure of the target substance, its

functional groups and the solubility data give

us valuable hints for the choice of the

screening phases.

Step 2: For the selected phase, a protocol is

implemented for your application. Then

samples and / or prototypes are delivered to

you for testing, evaluation and validation.

Step 3: When the selected phase suits your

application and has been validated, a scale-up

is planned. A QC report is delivered with the

product. The format of the product is

correlated to your application and can be bulk

material, SPE cartridges, HPLC columns etc…

No matter if we run a short term project (2-3 days) or long term project ( 4 to 6 months) we

always follow a well-established procedure. The following scheme describes an example of a

procedure for the development of a custom-made product based on our customer’s

requirements.

After signing of Confidential Disclosure Agreements (CDA), key data are exchanged. A

quotation based on our experience in separation science including a process sheet is

developed. During the whole procedure, a permanent feedback is established with you.

PROJECT DEVELOPMENT

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NOTES

About AFFINISEPAFFINISEP is a worldwide expert in purification and sample preparation applications as well as for

the design and the development of intelligent polymers with Molecularly Imprinted Polymers (MIP).

AFFINISEP is dedicated to the development of analytical applications in various fields such as water,

biological fluids, food and feed analysis with a complete set of products and services for sample

preparation:

SPE cartridges

On-line SPE columns

QuEChERS

Standards

Our mission is to develop and market innovative products of high value to customers by a practical

contribution to their work. By offering you a most comprehensive range of solid phase extraction

products, AFFINIMIP® SPE products based on molecularly imprinted polymers, AttractSPETM a range

of polymeric phases and SilactSPETM Silica based products, associated reagents, QuEChERS and

small equipment, the analytical chemists can find any solution for sample preparation, selective

extraction and sample clean-up needs in various sectors: food and feed safety and quality,

pharmaceutical R&D and quality control, clinical diagnosis, environment and doping.

Furthermore, by exploiting our library of innovative polymers and our know-how in chromatography

and solid phase extraction, we have a strong capacity to adapt these polymers to meet any specific

requirements and to solve unsatisfied purification and extraction needs.

Numerous documents related to our products (Application notebooks, publication references,

posters, catalog for different applications…) can be found on our website www.affinisep.com.

For any order, please, choose one of the following ways:

By mail:

AFFINISEP

Pharma Parc II

Voie de l’innovation

27100 Val de Reuil, FRANCE

By fax: +33 (0)2 32 59 61 01

By email: [email protected]

On-line by E-commerce: To save your time and a quicker delivery,

order via our web site www.affinisep.com

Information about new products or new matrices:

Newsletters

Stay up-to-date with the news on AFFINISEP’s latest innovations by subscribing to our newsletter,

delivering new information straight to your inbox.

ORDERING INFORMATION

2181 N Tracy Blvd Ste 202, Tracy, CA 95376 USAphone: 1-347-688-4752 [email protected] www.columnnex.com

Columnex LLC

Columnex LLC represents all Affinisep products sold in America

www.polyintell.com

sample preparation catalog - hplc columns … preparation catalog 2 dear customer we are pleased to...

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