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![Page 1: rspb.royalsocietypublishing.orgrspb.royalsocietypublishing.org/.../rspb20133133supp1.docx · Web viewexpression in the fore- (F) and hindlimbs (H) of embryonic bats and mice visualized](https://reader036.vdocuments.site/reader036/viewer/2022070612/5b46dded7f8b9af54b8bceee/html5/thumbnails/1.jpg)
Electronic supplementary material (table S1 and figures S1-S7)
Table S1. Primers for making ISH probes.
Gene name Forward primer (5' → 3') Reverse primer (5' → 3') Product length (bp)
Bat_Hoxd9 TACGAGGTGGCAAGGATT TTCCAGGTCTCAGTCAAGG 629
Bat_Hoxd10 AGTAGATGTCGCTGTTGTCCGC AAGGGACCTCGGCAGAAATG 602
Bat_Hoxd11 TTCTTCCCACGGTCAACT GCAGCACCAGGATCACAT 607
Bat_Hoxd12 TTTCGTCCAATCGTCTGA CCTTGGAGTCGTCCTTGA 587
Bat_Hoxd13 ACATGGTGTCCACCTTCG AAAGTCAGGTTGGGTAGCA 590
Bat_Tbx3 TCCTCAACCTGAACACCAT AGATCCCGGATACAGAAACC 543
Bat_Fam5c GCGGAACCTGGGCTACAT AAGCATTGCCACGGAACA 630
Mouse_Hoxd9 GCCAGGATTCTGAACCTTACA GAGGCTGGATAGGGTGAGAC 511
Mouse_Hoxd10 CCGAGTCTTGTCCTGTTG GGTTTGTTTCCGCCTTGC 705
Mouse_Hoxd11 CTCGGATGCTCAACCTCAC ACAGTGACTCATGCCCAAAG 556
Mouse_Hoxd12 TGAGCCCGTCCTCCTATT CCACTTGCACTGCCATGTTC 599
Mouse_Hoxd13 CACTTCGGCAACGGTTACTA CCTTCACCCTTCGATTCTG 607
Mouse_Tbx3 GAAGTCAGGAAGGCGAATG TGGGCAAAGCAGTTGAAGG 609
Mouse_Fam5c CTGGGAAACAAATGGAAGA CAAGATTTTGGGTTGTGCT 493
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Figure S1. Alcian blue staining of bat and mouse autopods. Orientation of limbs as in figure 1. F:
forelimb; H: hindlimb; S16E: early stage 16; S17M: middle stage 17; S19L: late stage 19. Scale
bars, 500 μm.
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Figure S2. Hoxd10 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
![Page 4: rspb.royalsocietypublishing.orgrspb.royalsocietypublishing.org/.../rspb20133133supp1.docx · Web viewexpression in the fore- (F) and hindlimbs (H) of embryonic bats and mice visualized](https://reader036.vdocuments.site/reader036/viewer/2022070612/5b46dded7f8b9af54b8bceee/html5/thumbnails/4.jpg)
Figure S3. Hoxd11 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
![Page 5: rspb.royalsocietypublishing.orgrspb.royalsocietypublishing.org/.../rspb20133133supp1.docx · Web viewexpression in the fore- (F) and hindlimbs (H) of embryonic bats and mice visualized](https://reader036.vdocuments.site/reader036/viewer/2022070612/5b46dded7f8b9af54b8bceee/html5/thumbnails/5.jpg)
Figure S4. Hoxd12 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
![Page 6: rspb.royalsocietypublishing.orgrspb.royalsocietypublishing.org/.../rspb20133133supp1.docx · Web viewexpression in the fore- (F) and hindlimbs (H) of embryonic bats and mice visualized](https://reader036.vdocuments.site/reader036/viewer/2022070612/5b46dded7f8b9af54b8bceee/html5/thumbnails/6.jpg)
Figure S5. Hoxd13 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 500 μm.
![Page 7: rspb.royalsocietypublishing.orgrspb.royalsocietypublishing.org/.../rspb20133133supp1.docx · Web viewexpression in the fore- (F) and hindlimbs (H) of embryonic bats and mice visualized](https://reader036.vdocuments.site/reader036/viewer/2022070612/5b46dded7f8b9af54b8bceee/html5/thumbnails/7.jpg)
Figure S6. Tbx3 expression in the fore- (F) and hindlimbs (H) of embryonic bats and mice
visualized by ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-L) and 500 μm in
(M-X).
![Page 8: rspb.royalsocietypublishing.orgrspb.royalsocietypublishing.org/.../rspb20133133supp1.docx · Web viewexpression in the fore- (F) and hindlimbs (H) of embryonic bats and mice visualized](https://reader036.vdocuments.site/reader036/viewer/2022070612/5b46dded7f8b9af54b8bceee/html5/thumbnails/8.jpg)
Figure S7. Fam5c expression in the fore- (F) and hindlimbs (H) of embryonic bats visualized by
ISH. Orientation of limbs as in figure 1. Scale bars, 200 μm in (A-H) and 500 μm in (I-P).