report of the statistics committee

2011 – 2012 report

Statistics Committee

Wednesday, June 13, 2012

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• STA Committee membership:Chair: Jean-Louis Laffont FranceVice: Kirk Remund USAMembers: Julianna B nyai Hungary

Julia Barabas HungaryOlfat H. El Bagoury EgyptMustapha El Yakhlifi FranceMohamed Hanafi FranceBonnie Hong USAWinfried Jackisch GermanyZivan Karaman FranceTim Perez USA

Gabriel CarréFrance

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Overview of support activities from June 2011 to June 2012:

Report reviews

Statistical analyses

Consulting

Statistical tools

Workshops/Seminars

Support to GMO Committee activities

Announcement: 9th ISTA Seminar on Statistics in Seed Testing

Working programme June 2012 – June 2013

Outline

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Report reviews: 4 test plan reviews,1 validation report review

Statistical analysis: 2 Method Validationstudies (germination), 1 Comparative Test

(June 2010 – June 2011: 8 test plan reviews,8 validation report reviews

June 2009 – June 2010: 3 test plan reviews,2 validation report reviews

June 2008 – June 2009: 8 test plan reviews,8 validation report reviews)

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Consulting:

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Statistical tools

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Statistical tools:

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Statistical tools:

… also from:

Number of downloads for the period 01/06/2011 31/05/2012

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Number of stat tools downloads for theperiod 01/06/2011 31/05/2012

Grand total: 2818

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Statistical tools: 2 new tools published

8 downloads since publishing date (21/11/12)New version in preparation

Thanks for the support (testing, documentation)from the Bulking and Sampling Committee, inparticular Leena Pietilä and Dot Vittrup Pedersen.

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Box plots Excel macro

Microsoft® Excel is widely used for graphing data, but has no included method for creating box plots in the style of Tukey (1977).

There are numerous commercial or free Excel add-ins and macrosfor creating box plots… which are not fully satisfactory.

The macro developed for ISTA provides an easy way to producehigh-quality box plots.

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Box plots Excel macro

The box plot is used to portray the distribution of a set of data.

Box plots are useful for:Data checkingComparing distributions

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Box plots Excel macro: input data

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Box plots Excel macro: output

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Box plots Excel macro: next version (thanks to Gabriel Carré)

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Heterogeneity Testing Calculator for seed lots in multiple containers

To support Chapter 2.9 of the ISTA Rules

Microsoft® Excel workbook with 9 worksheets:

Purity worksheets: Total pure seedsPurity-Inert matterPurity-Other seeds

Germination worksheets: Germ-Normal SeedlingsGerm-Abnorm SeedsGerm-Dead Seeds

Other seed worksheets: Total number of other seedsNumber of species with the highest numberNumber of species with the second-highest number

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Heterogeneity Testing Calculator for seed lots in multiple containers

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Workshops/Seminars

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ISTA Workshop on Statistical Aspects of GMO Detection - Mexico

Location: Autonomous Metropolitan University, Mexico City

Date: July 25-29, 2011Organizers: Dr. Adriana Otero Arnaiz &

Dr. Martha Graciela Rocha Munive

Lecturers: Benjamin Kaufman, Jean-Louis Laffont & Kirk Remund

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22 participants from Mexico, Uruguay, France, Canada,Kenya and United States

Statistics Topics Useful statistical tools in GMO testing: ANOVA, box plots, QQ plots, …

Qualitative & quantitative seed testing plans - SeedcalcData checkingMeasurement uncertainty NEW!

ISTA Workshop on Statistical Aspects of GMO Detection - Mexico

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ISTA Workshop on Biotechnology Trait Detection - China

Location: Shanghai Jiao Tong University

Date: December 5-9, 2011Organizers: Dr. Dabing Zhang &

Dr. Litao YangLecturers: Benjamin Kaufman,

Bruno Zaccomer, Cheryl Dollard, Kirk Remund

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23 participants from China, Taiwan, Brazil, South Korea, Croatia and United States

Statistics TopicsQualitative & quantitative seed testing plans using SeedcalcGM impurity estimation using SeedcalcNew Tools: box plot macro


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We had lots of fun!!

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Participation (JL Laffont) in:ISTA SEED HEALTH & STATISTICS SEMINAR

Paris, 22 May 2012

Working areas for statisticians:Homogeneity testStability testSH PT rating: binary dataGeneralized Linear Mixed Model for the analysis of proportions: theoretical developments, tools

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STA contributions to ISTA GMO Testing Rules Chapter

Calculations and expression of results sectionDefinition of seed lot population

Testing objectives

Testing methods

ISTA tools for results calculation

269th ISTA Seminar on Statistics in Seed Testing

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Location: Genotyping/Botany, Ottawa Plant Lab, Canadian Food Inspection Agency (Organizer: Cheryl Dollard)

Dates: September 26-28, 2012

Lecturers: Jean-Louis Laffont, Kirk Remund, Bonnie Hong, Tim Perez, Zivan Karaman, Gabriel Carré

Topics: Statistical Methods for Germination, Uncertainty for low level presence (LOD, LOQ), Tools in R and MS Excel, EFSA Methods, Equivalence Testing, Seedcalc, Tolerance Calculation Tools

9th ISTA Seminar on Statistics in Seed Testing

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Working programmeJune 2012 – June 2013

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Continue to deliver report reviews, statisticalanalyses, stat consultancy (e.g. answering questions)

Working programme - June 2012 – June 2013

Support

Specific support to:Germination Committee: analysis of in-housegermination method validation study, …GMO Committee: GMO PT rating, GMO PT ratingenhancements, ISTA GMO Testing Rules Chapter,…Bulking and Sampling Committee: check sampling,sample size for other seed count in the heterogeneity test, …

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Workshops / Seminars

ISTA Seed Health & Statistics SeminarSpring 2013

9th ISTA Seminar on Statistics in Seed TestingOttawa – September 26-28, 2012

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New version of the box plot macro

Analysis of germination validation studies

Seedcalc and SeedcalcStack update


Excel tools

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Acknowledgements

• STA Committee members

• ISTA Secretariat and ISTA ECOM

• TCOM members

• Users of the tools developed by the STA Committee

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Thank you for your attention

www.seedtest.org

report of the statistics committee

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