relevant in vitro toxicity safety testing for the cosmetic...
TRANSCRIPT
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Relevant In Vitro Toxicity Safety Testing for the Cosmetic Industry
Jamin A. Willoughby, Sr. Ph.D.
New England Chapter - Society of Cosmetic Chemists
Annual Scientific Seminar
October 6, 2016
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Overview
Who we are and what we do
General toxicity concerns in the cosmetics industry
• Regulatory requirements.
• How do we proceed intelligently safety testing
Non-animal methods for assessing relevant toxicity endpoints
• Ocular Irritation
• Dermal Corrosion
• Dermal Irritation
• Dermal Sensitization
Discussion/Conclusion
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Cyprotex
CRO with three locations worldwide
• Macclesfield, UK
• Watertown, MA USA
• Kalamazoo, MI USA (GLP)
Kalamazoo site has specific expertise
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Standard Components of an NDA
Index
Summary
Chemistry, Manufacturing, and
Control;
Samples, Methods Validation
Package, and Labeling;
Nonclinical Pharmacology and
Toxicology;
Human Pharmacokinetics and
Bioavailability;
Microbiology (for anti-
microbial drugs only);
Clinical Data;
Safety Update Report
Statistical;
Case Report Tabulations;
Case Report Forms;
Patent Information;
Patent Certification; and
Other Information.
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Standard Components of an NDA
Index
Summary
Chemistry, Manufacturing, and
Control;
Samples, Methods Validation
Package, and Labeling;
Nonclinical Pharmacology and
Toxicology;
Human Pharmacokinetics and
Bioavailability;
Microbiology (for anti-
microbial drugs only);
Clinical Data;
Safety Update Report
Statistical;
Case Report Tabulations;
Case Report Forms;
Patent Information;
Patent Certification; and
Other Information.
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Nonclinical Pharmacology and Toxicology
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Toxicity Testing for Cosmetics
Unlike other industries, there is no regulation.
“Under the law, cosmetic products and ingredients do not need
FDA premarket approval, with the exception of color additives.
However, FDA can pursue enforcement action against products
on the market that are not in compliance with the law, or against
firms or individuals who violate the law.”
“Companies and individuals who manufacture or market
cosmetics have a legal responsibility to ensure the safety of their
products. Neither the law nor FDA regulations require specific
tests to demonstrate the safety of individual products or
ingredients.”
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So how do we proceed intelligently?
Although there are no required specific tests,
standard methods that are applicable to the
cosmetics industry would be helpful.
Non-Animal Methods (correlation, ethics,
marketing).
Thankfully, the EU has already presented a path
we can follow/adapt for our uses.
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What has the EU Done?
Cosmetics Europe
• An established European association representing over
4000 member companies and associations of different
sizes in the cosmetics and personal care industry.
• Been around for more than 50 years.
• It requires cosmetics to cause no damage to human health.
• Set standards for consumer safety, which are governed by
the EU Cosmetics Regulations.
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EU Cosmetics Regulations
List of approved substances.
List of banned substances (1132 chemicals), restricted
substances (95 chemicals), provisionally allowed substances
(60 chemicals), coloring agents allowed or provisionally
allowed, preservatives allowed or provisionally allowed, UV
filtering agents allowed, etc.
Labelling requirements to provide consumers with access to
product information.
List of approved non-animal methods for assessing product
safety.
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Why Only Non-Animal Methods?
Testing ban on finished cosmetic products (Sept. 11, 2004).
Testing ban on ingredients or combination of ingredients
(March 11, 2009).
Prohibition to market finished cosmetic products and
ingredients in the EU which were tested on animals (March
11, 2013).
Therefore, The European Centre for the Validation of
Alternative Methods (ECVAM) plays a key role in the
development, validation, and international recognition of
alternative methods which reduce, refine, or replace the use
of animals in testing.
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So What? We are not the EU . . .
Gives us a path forward for using standardized non-animal methods.
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This Path Forward is Useful
Gives us a path forward for using standardized non-animal methods.
Marketing
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This Path Forward is Useful
Gives us a path forward for using standardized non-animal methods.
Marketing
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This Path Forward is Useful
Gives us a path forward for using standardized non-animal methods.
Marketing
Ethics
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This Path Forward is Useful
Gives us a path forward for using standardized non-animal methods.
Marketing
Ethics
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This Path Forward is Useful
Gives us a path forward for using standardized non-animal methods.
Marketing
Ethics
Correlation
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This Path Forward is Useful
Gives us a path forward for using standardized non-animal methods.
Marketing
Ethics
Correlation
![Page 19: Relevant In Vitro Toxicity Safety Testing for the Cosmetic ...newenglandscc.org/wp-content/uploads/2016/10/NESCC-Seminar__JAWS__2016_Final.pdfRelevant In Vitro Toxicity Safety Testing](https://reader035.vdocuments.site/reader035/viewer/2022070715/5ed86abdb7394006ce6027f1/html5/thumbnails/19.jpg)
This Path Forward is Useful
Gives us a path forward for using standardized non-animal methods.
Marketing
Ethics
Correlation
![Page 20: Relevant In Vitro Toxicity Safety Testing for the Cosmetic ...newenglandscc.org/wp-content/uploads/2016/10/NESCC-Seminar__JAWS__2016_Final.pdfRelevant In Vitro Toxicity Safety Testing](https://reader035.vdocuments.site/reader035/viewer/2022070715/5ed86abdb7394006ce6027f1/html5/thumbnails/20.jpg)
I’m getting bored, get to the point
When ECVAM validates non-animal methods, they are often also adopted by the OECD as Test Guidelines (protocols).
The United States is also an OECD country.
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Why Do We Care About OECD?
OECD Test Guidelines are “accepted
internationally as standard methods for
safety testing.”
Regularly updated with the assistance of
thousands of national experts from OECD
member countries.
OECD Test Guidelines are covered by the
Mutual Acceptance of Data.
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Still bored, get to the point
Although toxicity testing of cosmetics in the US is not regulated, the EU cosmetics industry IS regulated, and the ECVAM and OECD have developed, validated and standardized specific non-animal methods.
Companies in the US can use these methods to build a standard toxicity testing strategy.
The outcome (data) from these tests are applicable to all OECD members if performed under the OECD Test Guidelines.
These methods often correlate to known human responses far better than animal models.
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Standard Tox Concerns - Cosmetics
Ocular Irritation
Dermal Corrosion
Dermal Irritation
Dermal Sensitization
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Ocular Irritation
Ocular Irritation is defined as “production of changes in the eye following the application of test substance to the anterior surface of the eye, which are fully reversible within 21 days of application.”
Historically performed using rabbits (Draize Eye Irritation Test – OECD 405).
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Ocular Irritation
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Ocular Irritation
Ocular Irritation is defined as “production of changes in the eye following the application of a test substance to the anterior surface of the eye, which are fully reversible within 21 days of application.”
Historically performed using rabbits (Draize Eye Irritation Test – OECD 405).
Use a weight-of-evidence approach.
Exhaust all in vitro methods if possible.
Use in vivo studies only as a last resort or if required by regulatory bodies.
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In Vitro Methods for Ocular Irritation
There are a number of in vitro alternatives . . . Which do I choose?
• STE assay
• SMI assay
• BCOP assay
• ICE assay
• Fluorescein Leakage assay
• RhCE assay
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Short Time Exposure (STE) Assay
Expose cells
to 5% and
0.05% of test
chemical
Rinse test
material off of
cells and
assess
viability (MTT)
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In Vitro Methods for Ocular Irritation
There are a number of in vitro alternatives . . . Which do I choose?
• STE assay
• SMI assay
• BCOP assay
• ICE assay
• Fluorescein Leakage assay
• RhCE assay
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Slug Mucosal Irritation (SMI) Assay
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In Vitro Methods for Ocular Irritation
There are a number of in vitro alternatives . . . Which do I choose?
• STE assay
• SMI assay
• BCOP assay
• ICE assay
• Fluorescein Leakage assay
• RhCE assay
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Bovine Corneal Opacity (BCOP) Assay
Based on opacity and leakage, a
score is generated and the score
correlates to a specific level of
irritation.
≤3 = No Category
>3 & ≤25 = No Prediction
>55 = Category 1
Opacity is measured via
opacitometer and
permeability is assessed
by fluorescein leakage Expose cornea
to test chemical
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In Vitro Methods for Ocular Irritation
There are a number of in vitro alternatives . . . Which do I choose?
• STE assay
• SMI assay
• BCOP assay
• ICE assay
• Fluorescein Leakage assay
• RhCE assay
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Isolated Chicken Eye (ICE) Assay
Based on opacity, swelling and
and leakage, a score is generated
and the score correlates to a
specific level of irritation.
Opacity, fluorescein
permeability and
corneal swelling are
measured
Expose cornea
to test chemical
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In Vitro Methods for Ocular Irritation
There are a number of in vitro alternatives . . . Which do I choose?
• STE assay
• SMI assay
• BCOP assay
• ICE assay
• Fluorescein Leakage assay
• RhCE assay
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Fluorescein Leakage Assay
Expose cells to
numerous
concentrations of
test chemical
Rinse cells and add
sodium fluorescein
Measure the
amount of sodium
fluorescein in the
basal media
Concentration
causing 20%
leakage (FL20)
must be
≤ 100 mg/mL
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In Vitro Methods for Ocular Irritation
There are a number of in vitro alternatives . . . Which do I choose?
• STE assay
• SMI assay
• BCOP assay
• ICE assay
• Fluoroscein Leakage assay
• RhCE assay
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It’s not just me . . . I swear Consortium for Eye Irritation (CON4EI)
Formed by Flemish Institute for Technological Research (VITO) in response to European Chemical Industry Council (Cefic) research initiative.
CON4EI identified 8 assays to assess
• STE assay
• SMI assay
• BCOP assay (2 distinct opacitometers)
• ICE assay
• RhCE assay (from two tissue manufacturers)
Goal: To assess the reliability of these in vitro tests, define applicability domains in terms of ‘drivers of classification’, strengths and limitations of each method. In this way, we will be able to identify methods that will fit in a tiered approach to fully and accurately classify chemicals/substances.
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It’s not just me . . . I swear Consortium for Eye Irritation (CON4EI)
Formed by Flemish Institute for Technological Research (VITO) in response to European Chemical Industry Council (Cefic) research initiative.
CON4EI identified 8 assays to assess
• STE assay
• SMI assay
• BCOP assay (2 distinct opacitometers)
• ICE assay
• RhCE assay (from two tissue manufacturers)
Goal: To assess the reliability of these in vitro tests, define applicability domains in terms of ‘drivers of classification’, strengths and limitations of each method. In this way, we will be able to identify methods that will fit in a tiered approach to fully and accurately classify chemicals/substances.
>90% specificity and
sensitivity
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Reconstituted Human Corneal Epithelia (RhCE)
Two manufacturers of RhCE tissues.
• EpiOcular™ (MatTek Corporation)
• HCE (SkinEthic/L’Oreal)
Only the MatTek model is OECD validated (TG 492)
Human Cornea
EpiOcular™
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Reconstituted Human Corneal Epithelia (RhCE)
• Solubility of material is not a concern
• Similar phenotype to in vivo
• Uses human cells
• Metabolically active
• Can correct for test material
interference of viability assay (MTT)
• Can correct for color interference
• Disadvantage is lack of tearing/blinking
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Reconstituted Human Corneal Epithelia (RhCE)
Expose tissues to test article (neat)
Neg Control – Water
Pos Control – Methyl Acetate
Read OD of solubilized formazan
Incubate at 37°C, 5% CO2
Rinse all the test material off tissues
Incubate at
37°C, 5% CO2 Solubilize/extract in
isopropanol
30 min for liquids and 6 hours
for solids
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Reconstituted Human Corneal Epithelia (RhCE)
In Vitro Results In Vivo Prediction
Mean tissue viability ≤ 50% Irritant (I), R38
Mean tissue viability > 50% Non-irritant (NI)
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Reconstituted Human Corneal Epithelia (RhCE)
In Vitro Results In Vivo Prediction
Mean tissue viability ≤ 50% Irritant (I), R38
Mean tissue viability > 50% Non-irritant (NI)
ET50 methods (though not OECD validated) also can be used.
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Reconstituted Human Corneal Epithelia (RhCE)
In Vitro Results In Vivo Prediction
Mean tissue viability ≤ 50% Irritant (I), R38
Mean tissue viability > 50% Non-irritant (NI)
ET50 methods (though not OECD validated) also can be used.
Neat ET50 method
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Reconstituted Human Corneal Epithelia (RhCE)
In Vitro Results In Vivo Prediction
Mean tissue viability ≤ 50% Irritant (I), R38
Mean tissue viability > 50% Non-irritant (NI)
ET50 methods (though not OECD validated) also can be used.
Neat ET50 method
Dilution ET50 method (surfactant based solutions and is applicable to water-soluble materials with a specific gravity of > 0.95)
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Standard Tox Concerns - Cosmetics
Ocular Irritation
Dermal Corrosion
Dermal Irritation
Dermal Sensitization
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Dermal Corrosion
Dermal corrosion is defined as “the production of irreversible damage of the skin; namely, visible necrosis through the epidermis and into the dermis, following the application of a test substance for up to four hours.”
Pretty rare in cosmetics, but arise after a manufacturing error or misuse by the consumer.
Historically performed using rabbits (skin irritation & corrosion testing performed in a single assay)
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Dermal Corrosion
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Dermal Corrosion
Dermal corrosion is defined as “the production of irreversible damage of the skin; namely, visible necrosis through the epidermis and into the dermis, following the application of a test substance for up to four hours.”
Pretty rare in cosmetics, but arise after a manufacturing error or misuse by the consumer.
Historically performed using rabbits (skin irritation & corrosion testing performed in a single assay).
Use a weight-of-evidence approach.
Exhaust all in vitro methods if possible.
Use in vivo studies only as a last resort or if required by regulatory bodies.
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In Vitro Methods for Dermal Corrosion
There are three in vitro alternatives . . . Which do I choose?
• Transcutaneous Electrical Resistance (TER)
• In Vitro Membrane Barrier Test (CORROSITEX®)
• RHE
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Transcutaneous Electrical Resistance (TER)
• Rat skin from 28-30 day old
rats is the only validated skin
source.
• Put skin over PTFE tube.
• Insert PTFE tube into MgSO4.
• Expose rat skin for 24 hours at
ambient temp.
• Remove test material, add
MgSO4 and TER electrodes.
• Assess permeability of
sulforhodamine B dye.
• Calculate “yes/no” for
corrosion potential based on
TER, tissue damage and
permeability
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In Vitro Methods for Dermal Corrosion
There are three in vitro alternatives . . . Which do I choose?
• Transcutaneous Electrical Resistance (TER)
• In Vitro Membrane Barrier Test (CORROSITEX®)
• RHE
![Page 54: Relevant In Vitro Toxicity Safety Testing for the Cosmetic ...newenglandscc.org/wp-content/uploads/2016/10/NESCC-Seminar__JAWS__2016_Final.pdfRelevant In Vitro Toxicity Safety Testing](https://reader035.vdocuments.site/reader035/viewer/2022070715/5ed86abdb7394006ce6027f1/html5/thumbnails/54.jpg)
In Vitro Membrane Barrier Test (CORROSITEX®)
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In Vitro Methods for Dermal Corrosion
There are three in vitro alternatives . . . Which do I choose?
• Transcutaneous Electrical Resistance (TER)
• In Vitro Membrane Barrier Test (CORROSITEX®)
• RHE
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RHE Method for Dermal Corrosion Two manufacturers of RHE tissues.
• EpiDerm™ (MatTek Corporation)
• EpiSkin (SkinEthic/L’Oreal)
Basically identical protocols (focus on MatTek).
Human Epidermis EpiDerm™
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RHE Method for Dermal Corrosion
• Solubility of material is not a concern
• Similar phenotype to in vivo
• Uses human cells
• Metabolically active
• Can correct for test material interference of
viability assay (MTT)
• Can correct for color interference
• Disadvantage is more time consuming and
requires more lab equipment than other
assay
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RHE Method for Dermal Corrosion Expose tissues to test article (neat)
Neg Control – Water
Pos Control – 8N KOH
Read OD of solubilized formazan
Incubate at 37°C, 5% CO2
Rinse all the test material off tissues
Incubate at
37°C, 5% CO2 Solubilize/extract in
isopropanol
3 min and 1 hour
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RHE Method for Dermal Corrosion
In Vitro Mean Tissue Viability In Vivo Prediction
3 min <50% Corrosive
3 min ≥50% and 1 hour <15% Corrosive
3 min ≥50% and 1 hour ≥15% Non-Corrosive
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Dermal Irritation
Dermal irritation is defined as “production of reversible damage of the skin following the application of a test substance for up to 4 hours.”
Pretty rare in cosmetics, but arise after a manufacturing error or misuse by the consumer.
Historically performed using rabbits (skin irritation & corrosion testing performed in a single assay)
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Dermal Irritation
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Dermal Irritation
Dermal irritation is defined as “production of reversible damage of the skin following the application of a test substance for up to 4 hours.”
Pretty rare in cosmetics, but arise after a manufacturing error or misuse by the consumer.
Historically performed using rabbits (skin irritation & corrosion testing performed in a single assay)
Use a weight-of-evidence approach.
Exhaust all in vitro methods if possible.
Use in vivo studies only as a last resort or if required by regulatory bodies.
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In Vitro Methods for Dermal Irritation
There is a single validated in vitro alternatives . . .
RHE Method for Dermal Irritation
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RHE Method for Dermal Irritation Two manufacturers of RHE tissues.
• EpiDerm™ (MatTek Corporation)
• EpiSkin (SkinEthic/L’Oreal)
Basically identical protocols (focus on MatTek).
Human Epidermis EpiDerm™
![Page 65: Relevant In Vitro Toxicity Safety Testing for the Cosmetic ...newenglandscc.org/wp-content/uploads/2016/10/NESCC-Seminar__JAWS__2016_Final.pdfRelevant In Vitro Toxicity Safety Testing](https://reader035.vdocuments.site/reader035/viewer/2022070715/5ed86abdb7394006ce6027f1/html5/thumbnails/65.jpg)
RHE Method for Dermal Irritation
• Solubility of material is not a concern
• Similar phenotype to in vivo
• Uses human cells
• Metabolically active
• Can correct for test material interference of
viability assay (MTT)
• Can correct for color interference
• Disadvantage is this is the only option for
irritation . . . .
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RHE Method for Dermal Irritation Expose tissues to test article (neat)
Neg Control – PBS
Pos Control – 5% SDS
Read OD of solubilized formazan
Incubate at
37°C, 5%
CO2
Rinse all the test material off tissues
Incubate at
37°C, 5% CO2
Solubilize/extract in
isopropanol
1 hour
Post-
exposure
recovery
period of 42
hours where
tissues sit in
media to
“recover” per
definition of
irritation
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RHE Method for Dermal Irritation
In Vitro Results In Vivo Prediction
Mean tissue viability ≤ 50% Irritant (I), R38
Mean tissue viability > 50% Non-irritant (NI)
ET50 methods (though not OECD validated) also can be used.
ET50 (hours) Expected in vivo Irritancy Example
<0.5 Strong/Severe, possible corrosive Nitric Acid
0.5 - 4 Moderate 1% SDS
4 - 12 Moderate to Mild 1% Triton X-100
12 - 24 Very Mild Baby Shampoo
>24 Non-irritating 10% Tween 20
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Dermal Sensitization
Dermal sensitization is defined as “an allergic reaction to a substance that results in the development of skin inflammation and itchiness. Unlike skin irritation, the skin becomes increasingly reactive to the substance as a result of subsequent exposures.”
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Dermal Sensitization
Dermal sensitization is defined as “an allergic reaction to a substance that results in the development of skin inflammation and itchiness. Unlike skin irritation, the skin becomes increasingly reactive to the substance as a result of subsequent exposures.”
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Dermal Sensitization
Dermal sensitization is defined as “an allergic reaction to a substance that results in the development of skin inflammation and itchiness. Unlike skin irritation, the skin becomes increasingly reactive to the substance as a result of subsequent exposures.”
Historically assessed using Guinea Pigs (Guinea Pig Maximization Test or Buehler Test) or Mice (Local Lymph Node Assay)
Very recently OECD guidelines have come out for alternative in vitro assays
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Dermal Sensitization
2 phases: Induction (with hapten formation) and Elicitation
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Dermal Sensitization
NQ01
AKR
TXN
IL8
Chemical
Sensitizers
Maf
Keap 1
NrF2
NrF2
ARE/EpRE
NrF2
Proteosomal
Degradation
of Nf2
Maf
Keap 1
NQ01
AKR
TXN
IL8
Chemical
Sensitizers
Maf
Keap 1
NrF2
NrF2
ARE/EpRE
NrF2
Proteosomal
Degradation
of Nf2
MafMaf
Keap 1
MafNrf1
MTF1
GSH
ROSMetals
MRE
Nrf1
ARE/EpRE
MTF1
MafMT1
MT2
MT1
MT2
MafMafNrf1
MTF1
GSH
ROS
GSH
ROSMetals
MRE
Nrf1
ARE/EpRE
MTF1
MafMT1
MT2
MT1
MT2
MT1
MT2
MT1
MT2XRE
Menadione
CYP1A1/2
Reactive
Metabolites
ArntAhR
XRE
Menadione
CYP1A1/2
Reactive
Metabolites
ArntArntAhR
Induction
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In Vitro Methods for Dermal Sensitization
Dermal sensitization is a highly complex process.
There are three in vitro alternatives . . . Which do I choose?
• KeratinoSens
• DPRA
• h-CLAT
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KeratinoSens Dermal Sensitization
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KeratinoSens Dermal Sensitization
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In Vitro Methods for Dermal Sensitization
Dermal sensitization is a highly complex process.
There are three in vitro alternatives . . . Which do I choose?
• KeratinoSens
• DPRA
• h-CLAT
![Page 77: Relevant In Vitro Toxicity Safety Testing for the Cosmetic ...newenglandscc.org/wp-content/uploads/2016/10/NESCC-Seminar__JAWS__2016_Final.pdfRelevant In Vitro Toxicity Safety Testing](https://reader035.vdocuments.site/reader035/viewer/2022070715/5ed86abdb7394006ce6027f1/html5/thumbnails/77.jpg)
Direct Peptide Reactivity Assay (DPRA)
Your Chemical
+
lysine cysteine
Measure decrease in free
amino acid by HPLC
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Direct Peptide Reactivity Assay (DPRA)
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In Vitro Methods for Dermal Sensitization
Dermal sensitization is a highly complex process.
There are three in vitro alternatives . . . Which do I choose?
• KeratinoSens
• DPRA
• h-CLAT
![Page 80: Relevant In Vitro Toxicity Safety Testing for the Cosmetic ...newenglandscc.org/wp-content/uploads/2016/10/NESCC-Seminar__JAWS__2016_Final.pdfRelevant In Vitro Toxicity Safety Testing](https://reader035.vdocuments.site/reader035/viewer/2022070715/5ed86abdb7394006ce6027f1/html5/thumbnails/80.jpg)
H-CLAT Dermal Sensitization
Expose THP-1
cells to test
chemical
Flow
cytometry to
assess CD54
and CD86
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H-CLAT Dermal Sensitization
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In Vitro Methods for Dermal Sensitization
We don’t choose any of them . . .
SensCeeTox Platform
MatTek EpiDerm tissues (96-well format)
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SenCeeTox® Assay
Developed by CeeTox, Inc.
Uses HaCaT cells or 3D RHE tissues
Dose response curve: 0.1 to 2500 µM
Assesses chemical reactivity (glutathione
reactivity), tissue/cell viability, expression of
Nrf1/2, MRE and AhR mediated genes
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NQO1 – NADPH-quinone oxidoreductase 1
AKR1C2 – Aldoketoreductase
IL-8 – Interleukin 8
CYP1a1 – Cytochrome P450
ALDH3A – Aldehyde dehydrogenase 3A
HMOX1 – Heme-oxygenase 1
GCLC – Glutamate cysteine ligase catalytic subunit C
TXN – Thioredoxin
MAFF – v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F
MT1 – Metallothionein 1
MT2 – Metallothionein 2
Genes Assessed in SenCeeTox® Assay
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All data is run through a proprietary algorithm that generates an estimated in vivo LLNA EC3 score.
SenCeeTox® Assay
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Summary
In vitro testing is what all the “cool kids” are doing.
No longer just cells on a plate covered in media.
Have platforms for the most commonly desired toxicity testing in cosmetics (ocular irritation, dermal irritation, dermal corrosion and dermal sensitization)
Platforms are valid for most all cosmetics formulations.
There are 3D models for toxicity testing of most organs of interest cosmetic chemistry world. • Respiratory
• Oral
• Gingival
• Vaginal
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Conclusion
US Regulatory bodies do not regulate cosmetics.
Hard to identify a straight forward or “standardized” path for in vitro testing of cosmetics.
Using EU regulations of cosmetics (and trial and error) Cyprotex has identified the optimal models for assessing ocular irritation, dermal irritation, dermal corrosion and dermal sensitization.
Use 3D models made of normal human cells • No need to worry about solubility/compatibility
• Can assess pure compounds, mixtures, final formulations, powders, creams, nano-particles, gels etc.
• Assessing human response in human tissues
• Rapid and inexpensive compared to in vivo
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Thank You
Karl Popp
NESCC
Acknowledgements
• Dr. Renee Zaya
• Dr. Brandon Zeigler
• Benjamin Meyer
• Lisa Blakeman
• Emily Manzon
• Jessica Sinha
• Donald Keller
Questions?