relation between external color and nutritional value of ... · ... of the different anthocyanins...

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Studies about chemical characteristics of sweet cherry fruits are very interesting because they allow the identification of the optimum point of ripening for harvesting and enable delivery of fruit to consumers in its best condition in terms of nutritional, sensory and functional properties [1] . Sweetness and skin color influence consumer acceptance of cherry cultivars, as do fruit weight and firmness [2] . The aim of this work was to study the evolution of chemical parameters, phenols and total antioxidant activity of ‘Sweetheart’ cherry in three distinct commercial ripening stages. Relation between external color and nutritional value of different ripening stages of ‘Sweetheart’ sweet cherry Agostinho, J.; Rodrigues, S.; Rato, A.E.; Agulheiro-Santos, A.C. Departamento de Fitotecnia, Escola de Ciências e Tecnologia, Universidade de Évora, Polo da Mitra, Ap.94, 7002-554 Évora, Portugal. Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Instituto de Investigação e Formação Avançada (IIFA), As expected maturation index increase according to ripening stage, being more noticeable the difference from stage 1 to stage 2. The color characteristics of the ‘Sweetheart’ sweet cherry, showed marked declines (p < 0, 05) from stage 1 through stage 3, corresponding to a more intense and darker color. According to Ballistreri et al. (2013) the color of cherries may be influenced by concentration and distribution of the different anthocyanins and colorless phenolics as well as the pH. The values observed of total phenol content show a deep increase from the cherries of ripening stage 2 comparing with those of ripening stage 3, consequently the antioxidant activity increase similarly. According to Hassimoto et al. (2005), the final antioxidant activity values are classified as high 73, 8 %. So should be considered the nutritional interest of the consumption of dark cherries. The visual appearance of ‘Sweetheart’ cherry, mainly color, should be a good criterion to choose cherries with good intrinsic quality showed by total phenols and DPPH. Total Phenols (Singleton, 1965) DPPH (Brand-Williams, 1995) External color (CIE L*a*b*) Total soluble solids (TSS) (°Brix) TA (g malic acid/100 g fresh weight) 0 10 20 30 40 50 60 1 2 3 C* Ripening stage 0 5 10 15 20 25 30 35 1 2 3 h angle Ripening stage 0 5 10 15 20 25 30 35 1 2 3 Maturation index Ripening stage 0 10 20 30 40 50 60 70 1 2 3 Total phenols (mg galic acid / 100g fruit) Ripening stage 0 20 40 60 80 100 1 2 3 DPPH (% Antioxidant capacity) Ripening stage [1] Serradilla MJ, Lozano M, Bernalte, MJ, Ayuso MC, Lópes-Corrales M, González-Gomez D (2011) Physicochemical and bioactive properties evolution during ripening of ‘Ambrunés’ sweet cherry cultivar. LWT - Food Science and Technology, 44, 199-205. [2] Ballistreri G, Continella A, Gentile A, Amenta M, Fabroni S, Rapisarda P. (2013) Fruit quality and bioactive compounds relevant to human health of sweet cherry (Prunus avium L.) cultivars grown in Italy, Food Chemistry, 140 , 630–638. [3] Singleton VL., Rossi, JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158. [4] Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. Academic Press. Vol. 28, pp. 25- 30 (a) (b) (c) (a) (b) (c) (a) (a) (b) (a) (a) (b) This work has been partially financed by the MITTIC Project; Technological Modernisation and Innovation based on ICT in strategic and traditional sectors, is financed jointly by the European Regional Development Fund (ERDF), through the Operational Programme of Cross-border Cooperation Spain - Portugal (POCTEP) 2007-2013" The authors thanks to ICAAM (Institute of Mediterranean Agrarian and Ambient Sciences), from University of Évora.

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Page 1: Relation between external color and nutritional value of ... · ... of the different anthocyanins and colorless phenolics as well as the ... Colorimetry of total phenolics with phosphomolybdic-phosphotungstic

Studies about chemical characteristics of sweet cherry fruits are very interesting because they allow the identification of the optimum point of ripening for harvesting and enable delivery of fruit to consumers in its best condition in terms of nutritional, sensory and functional properties[1]. Sweetness and skin color influence consumer acceptance of cherry cultivars, as do fruit weight and firmness [2]. The aim of this work was to study the evolution of chemical parameters, phenols and total antioxidant activity of ‘Sweetheart’ cherry in three distinct commercial ripening stages.

Relation between external color and nutritional value of different ripening stages of ‘Sweetheart’ sweet cherry Agostinho, J.; Rodrigues, S.; Rato, A.E.; Agulheiro-Santos, A.C. Departamento de Fitotecnia, Escola de Ciências e Tecnologia, Universidade de Évora, Polo da Mitra, Ap.94, 7002-554 Évora, Portugal. Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Instituto de Investigação e Formação Avançada (IIFA),

As expected maturation index increase according to ripening stage, being more noticeable the difference from stage 1 to stage 2. The color characteristics of the ‘Sweetheart’ sweet cherry, showed marked declines (p < 0, 05) from stage 1 through stage 3, corresponding to a more intense and darker color. According to Ballistreri et al. (2013) the color of cherries may be influenced by concentration and distribution of the different anthocyanins and colorless phenolics as well as the pH. The values observed of total phenol content show a deep increase from the cherries of ripening stage 2 comparing with those of ripening stage 3, consequently the antioxidant activity increase similarly. According to Hassimoto et al. (2005), the final antioxidant activity values are classified as high 73, 8 %. So should be considered the nutritional interest of the consumption of dark cherries.

The visual appearance of ‘Sweetheart’ cherry, mainly color, should be a good criterion to choose cherries with good intrinsic quality showed by total phenols and DPPH.

• Total Phenols (Singleton, 1965) • DPPH (Brand-Williams, 1995)

• External color (CIE L*a*b*)

• Total soluble solids (TSS) (°Brix) • TA (g malic acid/100 g fresh weight)

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[1] Serradilla MJ, Lozano M, Bernalte, MJ, Ayuso MC, Lópes-Corrales M, González-Gomez D (2011) Physicochemical and bioactive properties evolution during ripening of ‘Ambrunés’ sweet cherry cultivar. LWT - Food Science and Technology, 44, 199-205. [2] Ballistreri G, Continella A, Gentile A, Amenta M, Fabroni S, Rapisarda P. (2013) Fruit quality and bioactive compounds relevant to human health of sweet cherry (Prunus avium L.) cultivars grown in Italy, Food Chemistry, 140 , 630–638. [3] Singleton VL., Rossi, JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-158. [4] Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. Academic Press. Vol. 28, pp. 25-30

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This work has been partially financed by the MITTIC Project; Technological Modernisation and Innovation based on ICT in strategic and traditional sectors, is financed jointly by the European Regional Development Fund (ERDF), through the Operational Programme of Cross-border Cooperation Spain - Portugal (POCTEP) 2007-2013" The authors thanks to ICAAM (Institute of Mediterranean Agrarian and Ambient Sciences), from University of Évora.