Methods in Enzymology

Volume 153

Recombinant DNA

Part D

Ray Wu

Lawrence Grossman

CONTRIBUTORS TO VOLUME 153 ix

PREFACE Xiii

NATHAN 0 . KAPLAN XV

VOLUMES IN SERIES XVii

Section I . Vectors for Cloning DN A

1. Production of Single-Stranded Plasmid DNA

JEFFREY VIEIRAAND JOACHIM MESSING

A "

2 . pKUN, Vectors for the Separate Production of Rout) N . H . KONINGS ,

Both DNA Strands of Recombinant Plasmids ELS J . M . VERHOEVEN ,AND BEN P . H . PEETERS

12

3 . Restriction Site Bank Vectors for Cloning in JOHN DAVISON ,

Gram-Negative Bacteria and Yeast

MICHEL HEUSTERSPREUTE ,

AND FRANcOISE BRUNEL

34

4. Plasmids for the Selection and Analysis of Pro- JURGEN BROSIUS AND

karyotic Promoters

JAMES R . LUPSKI

54.

5 . A X DNA Protocol Based on Purification of Phage CYNTHIA HELMS ,

on DEAE-Cellulose

JAMES E . DUTCHIK, AND

MAYNARD V . OLSON

69

6. Double cos Site Vectors : Simplified Cosmid

PAUL BATES

82Cloning

7. Improved in Vitro Packaging of ,A DNA

SUSAN M . ROSENBERG

95

8 . A Phage Vectors-EMBL Series

A . M . FRISCHAUF ,

N . MURRAY ,AND H . LEHRACH

103

9 . Plasmid and Phage Vectors for Gene Cloning and DAVID A. Hopwoon ,Analysis in Streptomyces

MERVYN J . BIBB ,

KEITH F . CHATER, AND

TOBIAS KIESER

11 6

10 . Cosmid Shuttle Vectors for Cloning and Analysis R . NAGARAJA RAO ,

of Streptomyces DNA

M . A . RICHARDSON ,

AND S . KUHSTOSS

166

11 . Host-Vector Systems for Gene Cloning in Cyano- C . J . KUHLEMEIER AN Dbacteria

G . A . VAN ARKEL

19 9

12 . Genetic Engineering of the Cyanobacterial Chro- SUSAN S . GOLDEN ,mosome

JUDY BRUSSLAN, AN DROBERT HASELKORN

21 5

13 . Conjugal Transfer of Plasmids to Cyanobacteria TERESA THIEL AN DC . PETER WOLK

23 2

14 . Site-Directed Chromosomal Rearrangements in RICHARD T . SUROSKYYeast

AND BIK-KWOON TYE

24 3

15 . Improved Vectors for Plant Transformation : Ex- S . G . ROGERS, H . J . KLEE ,

pression Cassette Vectors and New Selectable R . B . HORSCH, AN DMarkers

R. T . FRALEY

25 3

16. Vectors for Cloning in Plant Cells

R . DEBLAERE ,A . REYNAERTS, H . HÖFTE ,J.-P . HERNALSTEENS ,J. LEEMANS, AN DM . VAN MONTAGU

277

17 . Binary Ti Vectors for Plant Transformation and GYNHEUNG AN

292

Promoter Analysi s

18 . Detection of Monocot Transformation via Agro- PAUL J . J . HOOYKAAS AN Dbacterium tumefaciens

RoB A . SCHILPEROORT

305

19 . Direct Gene Transfer to Protoplasts of Dicotyle- RAYMOND D . SHILLIT odonous and Monocotyledonous Plants by a AND INGO POTRYKUS

31 3Number ofMethods, Including Electroporation

20 . Uptake of DNA and RNA into Cells Mediated by W . H . R . LANGRIDGE ,Electroporation

B . J . LI, AN DA . A . SZALAY

33 6

21. Electroporation of DNA and RNA into Plant Pro- MICHAEL FROMM ,

toplasts

JUDY CALLIS ,LOVERINE P . TAYLOR ,AND VIRGINIA WALBOT

35 1

22 . Cloning Vectors of Mitochondria) Origin for Eu- CHRISTINE LANG-HINRICH Skaryotes

AND ULF STAHL

36 6

Section II . Vectors for Expression of Cloned Gene s

23 . Short Homopeptide Leader Sequences Enhanced WING L . SUNG, FEI-L . YAO ,Production of Human Proinsulin in Escherichia AND SARAN A . NARANG

385call

24 . Expression of Bovine Growth Hormone Deriva- HANSEN M . HSIUNG AN D

tives in Escherichia coli and the Use of the De- WARREN C . MACKELLAR

390rivatives to Produce Natural Sequence Growt h

Hormone by Cathepsin C Cleavag e

25 . Expression of Eukaryotic Genes in Escherichia BRIGITTE E . SCHONER ,

coli with a Synthetic Two-Cistron System

RAMA M . BELAGAJE, AN D

RONALD G . SCHONER

40 1

26. Expression of Heterologous Unfused Protein in ERIK REMAUT ,

Escherichia coli

ANNE MARMENOUT ,

Guus SIMONS, AN D

WALTER FIERS

41 6

27. Directing Ribosomes to a Single mRNA Species : ANNA Hut ,

A Method to Study Ribosomal RNA Mutations PARKASH JHURANI, AN D

and Their Effects on Translation of a Single HERMAN A . DE BOER

43 2Messenger in Escherichia col i

28 . New Expression Vectors for Identifying and Test- KLAUS SCHNEIDER AN D

ing Signal Structures for Initiation and Termina- CHRISTOPH F . BECK

452tion of Transcription

29_ Synthesis and Sequence-Specific Proteolysis of KIYOSHI NAGAI AN D

Hybrid Proteins Produced in Escherichia coli HANS CHRISTIAN TH¢GERSEN 46 1

30 . Expression Plasmid Containing the X PL Promoter GREGORY MILMAN

48 2and c1857 Repressor

31 . Expression and Secretion of Foreign Proteins in Guy D . DUFFAUD ,

Escherichia coli

PAUL E . MARCH, AN D

MASAYORI INOUYE

49 2

32 . Engineering for Protein Secretion in Gram-Posi- SHING CHANG

50 7

tive Bacteria

33 . Expression and Secretion Vectors for Yeast

GRANT A . BITTER ,

KEVIN M . EGAN ,

RAYMOND A . KOSKI ,

MATTHEW O . JONES ,

STEVEN G . ELLIOTT, AND

JAMES C . GIFFIN

51 6

34 . Vaccinia Virus as an Expression Vector

ANTONIA PICCINI ,

MARION E . PERKUS ,

AND ENZO PAOLETTI

545

AUTHOR INDEX 565

SUBJECT INDEX 593