rebecca j. majus

1
RESEARCH POSTER PRESENTATION DESIGN © 2015 www.PosterPresentations.com “Cyanide is used as a suicidal agent but also as a homicidal agent, particularly among healthcare and laboratory workers, and it can potentially be used in a terrorist attack. It is also still used in cases of illegal euthanasia” (Musshoff, Kirschbaum, & Madea, 2011, p. 1). Although cyanide poisoning deaths are rare in the United States today, there are still people who have ready access to the poison through their occupations. These occupations include chemists, jewelers, those involved in pest control, mineral refining, photography, electroplating, dyeing, printing, and salmon poaching (Gill, Marker, & Stajic, 2004). The purpose of this research is to inform funeral professionals, or anyone interested, what cyanide poisoning is, how death from it occurs, how to detect it in postmortem remains, how it poses a problem for embalmers, and what embalming techniques can be used to treat it. Being able to determine postmortem identifying factors of cyanide poisoning without autopsy confirmation can be beneficial toward treating it correctly. With this knowledge, embalmers can then treat the decedent to look how the deceased's family remembered them. ABSTRACT OBJECTIVES [8] Cyanide is a rapidly acting, fatal chemical compound that consists of a carbon atom triple-bonded to a nitrogen atom (Department of Health, 2006). Numerous forms of cyanide exist: • Gaseous hydrogen cyanide (HCN) • Water-soluble potassium and sodium cyanide salts • Poorly water-soluble mercury, copper, gold, and silver cyanide salts (Department of Health, 2006). An individual can be exposed to cyanide through: • Inhalation of hydrogen cyanide gas • Ingestion of cyanide salts • Subcutaneous and intravenous injection (Department of Health, 2006). WHAT IS CYANIDE? Unnatural conditions caused by cyanide include: • Bright pink color of the blood • Intense livor mortis (intravascular blood discoloration) • Low blood viscosity • Rapid postmortem staining • Autopsies are often performed (Mayer, 2012). Livor mortis (Intravascular blood discoloration) • Can be distinguished by pressing on the discoloration. If discoloration lightens and the imprint of the fingers can be seen, this is a good test it’s intravascular and can be cleared • Livor can be removed by arterial injection or by elevating the affected tissues • Livor and stain can appear in the same tissues but livor precedes stain. • Refrigeration can speed hypostasis of the blood and the resulting livor mortis and post mortem stain • Brought about by gravitation of blood into the dependent capillaries. • First observed 20-30 minutes after death. (Mayer, 2012). HOW CYANIDE DEATHS AFFECT EMBALMING FLUIDS TO USE Multi-fluid Formula: C x V = C’ x V’ 24 (fluid index) x 40 (oz) = 2.5% (TDS/total diluted solution) x 384 (oz) • It will take 40 ozs (2.5 bottles) of a 24 index fluid to make three gallons of a 2.5% injection solution. Pre-Injection • 16 oz. of Proflow (1 bottle) Arterial Fluids • 40 oz. of Metasyn Accelerated (2.5 bottles) • 6 oz. of Coloro #2 • 20 oz. of Metaflow • 16 oz. of Rectifiant (1 bottle) • 302 oz. of Water (To fill to 3 Gallons) Cavity Fluids • 32 oz. of undiluted Cavity-N (28 Index) • (2 bottles: 1 up, 1 down) (thoracic, abdominal, pelvic). [3] [2] [4] REFERENCES [1] Department of Health. (2006, April). Retrieved September 09, 2017, from https://www.health.ny.gov/environmental/emergency/chemical_terrorism/cyanide_general.htm [2] Dodge. (n.d.). Metaflow. Retrieved October 10, 2017, from https://shop.dodgeco.com/en/chemicals [3] Dodge. (n.d.). Metasyn Accelerated. Retrieved October 19, 2017, from https://shop.dodgeco.com/en/chemicals [4] Dodge. (n.d.). Rectifiant. Firming. Retrieved October 10, 2017, from https://shop.dodgeco.com/en/chemicals [5] Everything you need, all in one place. (n.d.). Retrieved September 09, 2017, from http://www.thechampioncompany.com/product-catalog.html [6] Ganesan, K., Raza, S. K., & Vijayaraghavan, R. (2010). Chemical warfare agents. Journal of Pharmacy & Bioallied Sciences, 2(3). Retrieved September 8, 2017. [7] Gill, J. R., M.D., Marker, E., Ph. D., & Stajic, M., Ph. D. (2004). Suicide by cyanide: 17 deaths. Journal of Forensic Sciences, 49(4). Retrieved September 8, 2017. [8] Jcwf. (n.d.). Lewis-structuur van het cyanide ion [Photograph]. (Originally photographed 2009, December 21) [9] Judge, J. (1985). The black hole of Guyana the untold story of the Jonestown massacre . Retrieved September 8, 2017, from https://www.ratical.org/ratville/JFK/JohnJudge/Jonestown.pdf. [10] Know the threat you can't see. (n.d.). Retrieved September 09, 2017, from http://www.cyanideinsight.com/cyanide-poisoning/where-cyanide-is-found [11] Lindsay, A. (2006, April 19). Cyanide poisoning. Retrieved September 09, 2017, from https://www.thenakedscientists.com/articles/features/cyanide-poisoning [12] Mayer, J. S. (1986). Color and cosmetics: the consummation of restorative art. Dallas, Tex. (P.O. Box 4495, Dallas 75208): Professional Training Schools. [13] Mayer, R. G., & Reed, J. D. (2012). Embalming: History, theory, and practice. New York: Mcgraw-Hill Medical. [14] Musshoff, F., Kirschbaum, K. M., & Madea, B. (2011). An uncommon case of a suicide with inhalation of hydrogen cyanide. Forensic Science International, 204(1-3). Retrieved September 8, 2017. [15] Weerasinghe, D. S., MBBS, Perera, H. J., MD, & Abeyasinghe, N. L., MD. (2011). Case report – Death by subcutaneous injection of cyanide in Sri Lanka. Journal of Forensic and Legal Medicine, 18(4). Retrieved September 8, 2017. [16] Wolnik, K. A., Fricke, F. L., Bonnin, E., Gaston, C. M., & Satzger, R. D. (1984). The tylenol tampering incident - tracing the source. Analytical Chemistry, 56(3). doi:10.1021/ac00267a003 • Inform funeral professionals, or anyone interested, what cyanide poisoning is • Inform how death from cyanide biologically occurs. • Inform how to detect cyanide in postmortem remains. • Describe how cyanide poses a problem for embalmers. • Suggest embalming techniques which can be used to treat cyanide poisoned remains. • Being able to determine postmortem identifying factors of cyanide poisoning without autopsy confirmation can be beneficial toward treating it correctly. Department of Mortuary Science and Funeral Service, Southern Illinois University Carbondale Rebecca J. Majus Problems in Embalming: Cyanide Poisoning CYANIDE IN OUR HISTORY World War II: Holocaust • January 30, 1933 – May 8, 1945 • Hydrogen cyanide gas (Zyklon-B) • Millions of innocent civilians were killed by the Nazis with Zyklon-B gas during World War II. Zyklon-B pellets would vaporize when exposed to air. Originally intended for commercial use as a disinfectant and an insecticide, the Nazis discovered through experimentation the gas could be used to kill humans (Ganesan, Raza, & Vijayaraghavan, 2010). Jonestown Massacre • November 18, 1978 • Potassium cyanide • A community of mostly Blacks and women drank cyanide from paper cups of Kool-Aid as part of a mass suicide pact of the Jonestown camp. Adults and children died and fell around the main pavilion. Jones himself was shot in the head, an apparent suicide. For days, the body count mounted, from 400 to nearly 1,000. The bodies were flown to the United States and later cremated or buried in mass graves (Judge, 1985). Chicago Tylenol Murders • September 1982 • Potassium cyanide • “Seven people from suburban Chicago died after taking Extra Strength Tylenol capsules that had been maliciously adulterated with cyanide” (Wolnik et al., 1984, p. 1). (Personal photo) HOW CYANIDE KILLS • According to the Indian Journal of Pharmacology, cyanide poisoning is associated with a 95% mortality rate. It is one of the deadliest poisons because it kills the enzymes that transport oxygen throughout the body. Death occurs within minutes, literally starving the body of oxygen. • The natural history of severe acute cyanide poisoning is a lactic acidosis leading to respiratory failure, coma and death. Cyanide inhibits mitochondrial cytochrome oxidase, an enzyme system responsible for aerobic cellular respiration. It causes inhibition of aerobic and activation of anaerobic respiration leading to accumulation of lactate, producing metabolic acidosis. (Weerasinghe, D. S., MBBS, Perera, H. J., MD, & Abeyasinghe, N. L., MD., 2011). • Cyanide leads to histotoxic hypoxia by poisoning the systems that utilize oxygen to create energy and preventing them from using the oxygen. Even though there is plenty of oxygen there, the cells experience a lack of oxygen and are affected as if there was too little/no oxygen available (Karius, 2017). HOW CAN YOU DETECT A CYANIDE DEATH? The classic descriptions of the autopsy detection of cyanide ingestion include: • Pink lividity • Odor of “bitter almonds” • Gastritis • Oral/perioral erosions (Gill et al., 2004). Occupation of the deceased should be taken into account. This is because the use of cyanide to commit suicide by people who have easy access to it is predictable and could help solidify your suspicions. Specific occupations that use cyanide are: • Jewelers - use cyanide to rid gold of tarnish. • Salmon Poachers - poison salmon with a cyanide compound called Cymag. • Miners - use cyanide to separate various precious metals from ores. • Healthcare and laboratory workers (chemists) can also be suspected. (Gill et al., 2004). CASE STUDY A 40 year old, 5’5” and 130 pound housewife of a chemist was found lying in bed at her residence, no longer than 24 hours, with a suspicious beverage and letter on the beside table. She has bright pink lividity, oral purge, and smells of “bitter almonds.” The coroner determines suicide by cyanide poisoning to be the cause of death and does not require an autopsy to be performed. Refrigeration is not needed. 1) Elevate the head and shoulders to help drain blood from upper tissues. 2) Wash and shave remains. Disinfect orifices and set the features. 3) By palpating the discoloration and seeing a fingerprint appear, it known that the pink lividity is an intravascular blood discoloration which should clear during arterial injection and subsequent blood drainage. 4) Close the mouth using a needle injector and brads. 5) Cover the face with massage cream. 6) Select the right common carotid artery for injection and right internal jugular vein for drainage. 7) Drainage should be continuous until the livor mortis clears. Then, intermittent/alternate methods of drainage can be employed. Manual massage and mechanical raising/flexing of a body limb may assist in distribution and diffusion of the fluid. 8) Use sufficient pressure and rate of flow to uniformly distribute arterial solution. Begin at a slow rate of flow with high pressure. Once distribution is established, then a small increase in flow can better ensure distribution to distal body areas. 9) Pre-inject with a large enough volume to help clear livor mortis. 10) Arterial Embalm. 11) Aspirate - immediately after arterial injection using a trocar. 12) Cavity Embalm. 13) Pack the trachea with cotton using the purge preventative method to prevent any possible expulsion from the lungs/stomach. 14) Realign the features. (Mayer, 2012). 15) Remove massage cream and cosmetize with a transparent liquid or cream cosmetic. These may be clear or translucent (cloudy) as they appear in the container: applied thinly, they appear transparent and the skin is visible through them. Apply finishing powder last. (Mayer, 1986). EMBALMING STEPS CONCLUSION Being educated on how to determine postmortem identifying factors of cyanide poisoning, without autopsy confirmation, will help embalmers consider cyanide poisoning as being a possible cause of death when they see certain postmortem discolorations or smell a certain “bitter almond” scent that is associated with cyanide (Gill et al., 2004). With this knowledge, embalmers can create a memory picture of the decedent that their loved ones can cherish. ACKNOWLEDGEMENTS Special thanks to Dr. Thomas Shaw and the SIUC Mortuary Science and Funeral Service department for guidance in my research.

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Problems in Embalming- Cyanide Poisoningwww.PosterPresentations.com
“Cyanide is used as a suicidal agent but also as a homicidal agent, particularly among healthcare and laboratory workers, and it can potentially be used in a terrorist attack. It is also still used in cases of illegal euthanasia” (Musshoff, Kirschbaum, & Madea, 2011, p. 1). Although cyanide poisoning deaths are rare in the United States today, there are still people who have ready access to the poison through their occupations. These occupations include chemists, jewelers, those involved in pest control, mineral refining, photography, electroplating, dyeing, printing, and salmon poaching (Gill, Marker, & Stajic, 2004). The purpose of this research is to inform funeral professionals, or anyone interested, what cyanide poisoning is, how death from it occurs, how to detect it in postmortem remains, how it poses a problem for embalmers, and what embalming techniques can be used to treat it. Being able to determine postmortem identifying factors of cyanide poisoning without autopsy confirmation can be beneficial toward treating it correctly. With this knowledge, embalmers can then treat the decedent to look how the deceased's family remembered them.
ABSTRACT
OBJECTIVES
[8] Cyanide is a rapidly acting, fatal chemical compound that consists of a carbon atom triple-bonded to a nitrogen atom (Department of Health, 2006).
Numerous forms of cyanide exist: • Gaseous hydrogen cyanide (HCN) • Water-soluble potassium and sodium cyanide salts • Poorly water-soluble mercury, copper, gold, and silver cyanide salts (Department of Health, 2006).
An individual can be exposed to cyanide through: • Inhalation of hydrogen cyanide gas • Ingestion of cyanide salts • Subcutaneous and intravenous injection (Department of Health, 2006).
WHAT IS CYANIDE?
Unnatural conditions caused by cyanide include: • Bright pink color of the blood • Intense livor mortis (intravascular blood discoloration) • Low blood viscosity • Rapid postmortem staining • Autopsies are often performed (Mayer, 2012).
Livor mortis (Intravascular blood discoloration) • Can be distinguished by pressing on the discoloration. If discoloration lightens and the imprint of the fingers can be seen, this is a good test it’s intravascular and can be cleared • Livor can be removed by arterial injection or by elevating the affected tissues • Livor and stain can appear in the same tissues but livor precedes stain. • Refrigeration can speed hypostasis of the blood and the resulting livor mortis and post mortem stain • Brought about by gravitation of blood into the dependent capillaries. • First observed 20-30 minutes after death. (Mayer, 2012).
HOW CYANIDE DEATHS AFFECT EMBALMING FLUIDS TO USE
Multi-fluid Formula: C x V = C’ x V’ 24 (fluid index) x 40 (oz) = 2.5% (TDS/total diluted solution) x 384 (oz) • It will take 40 ozs (2.5 bottles) of a 24 index fluid to make three gallons of a 2.5% injection solution.
Pre-Injection • 16 oz. of Proflow (1 bottle)
Arterial Fluids • 40 oz. of Metasyn Accelerated (2.5 bottles) • 6 oz. of Coloro #2 • 20 oz. of Metaflow • 16 oz. of Rectifiant (1 bottle) • 302 oz. of Water (To fill to 3 Gallons)
Cavity Fluids • 32 oz. of undiluted Cavity-N (28 Index)
• (2 bottles: 1 up, 1 down) (thoracic, abdominal, pelvic).
[3] [2] [4]
REFERENCES [1] Department of Health. (2006, April). Retrieved September 09, 2017, from https://www.health.ny.gov/environmental/emergency/chemical_terrorism/cyanide_general.htm [2] Dodge. (n.d.). Metaflow. Retrieved October 10, 2017, from https://shop.dodgeco.com/en/chemicals [3] Dodge. (n.d.). Metasyn Accelerated. Retrieved October 19, 2017, from https://shop.dodgeco.com/en/chemicals [4] Dodge. (n.d.). Rectifiant. Firming. Retrieved October 10, 2017, from https://shop.dodgeco.com/en/chemicals [5] Everything you need, all in one place. (n.d.). Retrieved September 09, 2017, from http://www.thechampioncompany.com/product-catalog.html [6] Ganesan, K., Raza, S. K., & Vijayaraghavan, R. (2010). Chemical warfare agents. Journal of Pharmacy & Bioallied Sciences, 2(3). Retrieved September 8, 2017. [7] Gill, J. R., M.D., Marker, E., Ph. D., & Stajic, M., Ph. D. (2004). Suicide by cyanide: 17 deaths. Journal of Forensic Sciences, 49(4). Retrieved September 8, 2017. [8] Jcwf. (n.d.). Lewis-structuur van het cyanide ion [Photograph]. (Originally photographed 2009, December 21) [9] Judge, J. (1985). The black hole of Guyana the untold story of the Jonestown massacre . Retrieved September 8, 2017, from https://www.ratical.org/ratville/JFK/JohnJudge/Jonestown.pdf. [10] Know the threat you can't see. (n.d.). Retrieved September 09, 2017, from http://www.cyanideinsight.com/cyanide-poisoning/where-cyanide-is-found [11] Lindsay, A. (2006, April 19). Cyanide poisoning. Retrieved September 09, 2017, from https://www.thenakedscientists.com/articles/features/cyanide-poisoning [12] Mayer, J. S. (1986). Color and cosmetics: the consummation of restorative art. Dallas, Tex. (P.O. Box 4495, Dallas 75208): Professional Training Schools. [13] Mayer, R. G., & Reed, J. D. (2012). Embalming: History, theory, and practice. New York: Mcgraw-Hill Medical. [14] Musshoff, F., Kirschbaum, K. M., & Madea, B. (2011). An uncommon case of a suicide with inhalation of hydrogen cyanide. Forensic Science International, 204(1-3). Retrieved September 8, 2017. [15] Weerasinghe, D. S., MBBS, Perera, H. J., MD, & Abeyasinghe, N. L., MD. (2011). Case report – Death by subcutaneous injection of cyanide in Sri Lanka. Journal of Forensic and Legal Medicine, 18(4). Retrieved September 8, 2017. [16] Wolnik, K. A., Fricke, F. L., Bonnin, E., Gaston, C. M., & Satzger, R. D. (1984). The tylenol tampering incident - tracing the source. Analytical Chemistry, 56(3). doi:10.1021/ac00267a003
• Inform funeral professionals, or anyone interested, what cyanide poisoning is
• Inform how death from cyanide biologically occurs.
• Inform how to detect cyanide in postmortem remains.
• Describe how cyanide poses a problem for embalmers.
• Suggest embalming techniques which can be used to treat cyanide poisoned remains.
• Being able to determine postmortem identifying factors of cyanide poisoning without autopsy confirmation can be beneficial toward treating it correctly.
Department of Mortuary Science and Funeral Service, Southern Illinois University Carbondale Rebecca J. Majus
Problems in Embalming: Cyanide Poisoning
CYANIDE IN OUR HISTORY
World War II: Holocaust • January 30, 1933 – May 8, 1945 • Hydrogen cyanide gas (Zyklon-B) • Millions of innocent civilians were killed by the Nazis with Zyklon-B gas during World War II. Zyklon-B pellets would vaporize when exposed to air. Originally intended for commercial use as a disinfectant and an insecticide, the Nazis discovered through experimentation the gas could be used to kill humans (Ganesan, Raza, & Vijayaraghavan, 2010).
Jonestown Massacre • November 18, 1978 • Potassium cyanide • A community of mostly Blacks and women drank cyanide from paper cups of Kool-Aid as part of a mass suicide pact of the Jonestown camp. Adults and children died and fell around the main pavilion. Jones himself was shot in the head, an apparent suicide. For days, the body count mounted, from 400 to nearly 1,000. The bodies were flown to the United States and later cremated or buried in mass graves (Judge, 1985).
Chicago Tylenol Murders • September 1982 • Potassium cyanide • “Seven people from suburban Chicago died after taking Extra Strength Tylenol capsules that had been maliciously adulterated with cyanide” (Wolnik et al., 1984, p. 1).
(Personal photo)
HOW CYANIDE KILLS
• According to the Indian Journal of Pharmacology, cyanide poisoning is associated with a 95% mortality rate. It is one of the deadliest poisons because it kills the enzymes that transport oxygen throughout the body. Death occurs within minutes, literally starving the body of oxygen.
• The natural history of severe acute cyanide poisoning is a lactic acidosis leading to respiratory failure, coma and death. Cyanide inhibits mitochondrial cytochrome oxidase, an enzyme system responsible for aerobic cellular respiration. It causes inhibition of aerobic and activation of anaerobic respiration leading to accumulation of lactate, producing metabolic acidosis. (Weerasinghe, D. S., MBBS, Perera, H. J., MD, & Abeyasinghe, N. L., MD., 2011).
• Cyanide leads to histotoxic hypoxia by poisoning the systems that utilize oxygen to create energy and preventing them from using the oxygen. Even though there is plenty of oxygen there, the cells experience a lack of oxygen and are affected as if there was too little/no oxygen available (Karius, 2017).
HOW CAN YOU DETECT A CYANIDE DEATH? The classic descriptions of the autopsy detection of cyanide ingestion include: • Pink lividity • Odor of “bitter almonds” • Gastritis • Oral/perioral erosions (Gill et al., 2004).
Occupation of the deceased should be taken into account. This is because the use of cyanide to commit suicide by people who have easy access to it is predictable and could help solidify your suspicions.
Specific occupations that use cyanide are: • Jewelers - use cyanide to rid gold of tarnish. • Salmon Poachers - poison salmon with a cyanide compound called Cymag. • Miners - use cyanide to separate various precious metals from ores. • Healthcare and laboratory workers (chemists) can also be suspected. (Gill et al., 2004).
CASE STUDY A 40 year old, 5’5” and 130 pound housewife of a chemist was found lying in bed at her residence, no longer than 24 hours, with a suspicious beverage and letter on the beside table. She has bright pink lividity, oral purge, and smells of “bitter almonds.” The coroner determines suicide by cyanide poisoning to be the cause of death and does not require an autopsy to be performed. Refrigeration is not needed.
1) Elevate the head and shoulders to help drain blood from upper tissues.
2) Wash and shave remains. Disinfect orifices and set the features.
3) By palpating the discoloration and seeing a fingerprint appear, it known that the pink lividity is an intravascular blood discoloration which should clear during arterial injection and subsequent blood drainage.
4) Close the mouth using a needle injector and brads.
5) Cover the face with massage cream.
6) Select the right common carotid artery for injection and right internal jugular vein for drainage.
7) Drainage should be continuous until the livor mortis clears. Then, intermittent/alternate methods of drainage can be employed. Manual massage and mechanical raising/flexing of a body limb may assist in distribution and diffusion of the fluid.
8) Use sufficient pressure and rate of flow to uniformly distribute arterial solution. Begin at a slow rate of flow with high pressure. Once distribution is established, then a small increase in flow can better ensure distribution to distal body areas.
9) Pre-inject with a large enough volume to help clear livor mortis.
10) Arterial Embalm.
12) Cavity Embalm.
13) Pack the trachea with cotton using the purge preventative method to prevent any possible expulsion from the lungs/stomach.
14) Realign the features. (Mayer, 2012).
15) Remove massage cream and cosmetize with a transparent liquid or cream cosmetic. These may be clear or translucent (cloudy) as they appear in the container: applied thinly, they appear transparent and the skin is visible through them. Apply finishing powder last. (Mayer, 1986).
EMBALMING STEPS
CONCLUSION Being educated on how to determine postmortem identifying factors of cyanide poisoning, without autopsy confirmation, will help embalmers consider cyanide poisoning as being a possible cause of death when they see certain postmortem discolorations or smell a certain “bitter almond” scent that is associated with cyanide (Gill et al., 2004). With this knowledge, embalmers can create a memory picture of the decedent that their loved ones can cherish.