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  • 8/12/2019 Questions Bank for Molecular Biology (1)

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    !"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.

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    Your labmate was given a piece of DNA and told to make a map of the BamHI restriction enzymesites. The labmate digested the DNA with BamHI to completion and also did a partial digest. Hethen isolated fragments specifically created by the partial digest. These fragments were digestedwith BamHI to completion and the resulting products were run on a new gel. Below are pictures ofthe two gels (note: the size of each band in kilobases is indicated above the band), visualized with

    ethidium bromide. Help your labmate interpret the gel by drawing the resulting map. Show therelative location of each of the restriction enzyme sites, the number of nucleotides between the sitesand indicate if the DNA molecule is circular or linear.

    BamHI

    BamHI

    partial

    digest

    0.5

    4.5

    2.52.7

    5.0

    4.5

    0.7

    2.0

    0.7

    2.0

    0.5

    5.0

    fragment

    0.5

    4.5

    2.0

    0.7

    0.5

    2.7

    fragment

    2.5

    fragment

    3.2

    fragment

    3.2

    7.0

    7.0

    fragment

    2.0 2.0

    0.5

    0.7

    4.5

    2.0

    0.5

    uncut

    DNA

    7.7


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