qa course total
TRANSCRIPT
-
8/6/2019 QA Course Total
1/32
Quality is a Lousy IdeaQuality is a Lousy Idea--
If its Only an IdeaIf its Only an Idea
-
8/6/2019 QA Course Total
2/32
Quality Assurance vs.Quality Assurance vs.
Quality ControlQuality Control
Quality AssuranceQuality AssuranceAn overall
management plan to
guarantee the
integrity of data
(The system)
Quality ControlQuality ControlA series of
analytical
measurements used
to assess thequality of the
analytical data
(The tools)
-
8/6/2019 QA Course Total
3/32
True Value vs. MeasuredTrue Value vs. Measured
ValueValue
True ValueTrue Value
The known,accepted value of
a quantifiable
property
Measured ValueMeasured Value
The result of anindividuals
measurement of a
quantifiableproperty
-
8/6/2019 QA Course Total
4/32
Accuracy vs. PrecisionAccuracy vs. PrecisionAccuracyAccuracy
How well ameasurement
agrees with an
accepted value
PrecisionPrecision
How well a seriesof measurements
agree with each
other
-
8/6/2019 QA Course Total
5/32
Accuracy vs. PrecisionAccuracy vs. Precision
-
8/6/2019 QA Course Total
6/32
Systematic vs.Systematic vs.
Random ErrorsRandom ErrorsSystematic ErrorSystematic Error
Avoidable errordue to controllable
variables in a
measurement.
Random ErrorsRandom Errors
Unavoidable errorsthat are always
present in any
measurement.
Impossible toeliminate
-
8/6/2019 QA Course Total
7/32
Quality Control MeasuresQuality Control Measures Standards and Calibration
Blanks
Recovery Studies
Precision andAccuracy StudiesMethod Detection Limits
NJQLs
-
8/6/2019 QA Course Total
8/32
Standards and CalibrationStandards and Calibration
Prepared vs. PurchasedStandard
Signals: PeakArea, Beers Law
Calibration Curves
Continuing Calibration Checks
InternalStandards
Performance Testing.
-
8/6/2019 QA Course Total
9/32
Calibration CurvesCalibration CurvesGraphical representation of theGraphical representation of the
relationship between:relationship between:
The analytical signal
The concentration of the analyte
and
-
8/6/2019 QA Course Total
10/32
Calibration Curve for DDT
y = 9.3005x + 4.3313
0
100
200
300
400
500
0 10 20 30 40 50 60
Parts per trillion DDT
P
eak
area
x
106
R2
= 0.9989
-
8/6/2019 QA Course Total
11/32
Continuing CalibrationContinuing Calibration
VerificationVerification
Many methods dont require that
daily calibration curves areprepared
A
calibration verification isanalyzed with each batch of samples
-
8/6/2019 QA Course Total
12/32
Sample BatchSample Batch
10 - 20 samples (method defined)
or less
Same matrix
Same sample prep and analysis
Contains a full set ofQC samples
-
8/6/2019 QA Course Total
13/32
Internal StandardsInternal Standards
A compound chemically similar to
the analyte
Not expected to be present in thesample
Cannot interfere in the analysis
Added to the calibration standards
and to the samples in identical
amounts.
-
8/6/2019 QA Course Total
14/32
-
8/6/2019 QA Course Total
15/32
Performance TestingPerformance Testing
Blind samples submitted to
laboratories
?
?
?Labs must periodically
analyze with acceptableresults in order to maintain
accreditation
-
8/6/2019 QA Course Total
16/32
Blanks, Blanks, BlanksBlanks, Blanks, BlanksLaboratory Reagent Blanks
Instrument Blanks
Field Reagent Blanks
Trip Blanks
-
8/6/2019 QA Course Total
17/32
-
8/6/2019 QA Course Total
18/32
Instrument BlankInstrument Blank A clean sample (e.g., distilled water)
processed through the instrumentalsteps of the measurement process;
used to determine instrument
contamination.
-
8/6/2019 QA Course Total
19/32
Field Reagent BlanksField Reagent Blanks
Prepared in the lab, taken to the
field
Opened at the sampling site,
exposed to sampling equipment,
returned to the lab.
-
8/6/2019 QA Course Total
20/32
Trip BlanksTrip Blanks
Prepared in the lab, taken to the
field
Not opened
Returned to the lab
Not always required in EPA methods
-
8/6/2019 QA Course Total
21/32
RecoveryS
tudiesRecoveryS
tudiesMatrix Spikes
Laboratory ControlSamples
Surrogates .
-
8/6/2019 QA Course Total
22/32
Matrix SpikesMatrix Spikes
Sample spiked with a known
amount of analyte
Subjected to all sample prep and
analytical procedures
Determines the effect of the matrixon analyte recovery
Normally one per batch
-
8/6/2019 QA Course Total
23/32
Laboratory ControlS
ampleLaboratory ControlS
ample
Subjected to all sample prep andanalytical procedures
Analyte spiked into reagent water
-
8/6/2019 QA Course Total
24/32
Laboratory ControlS
ampleLaboratory ControlS
ampleAlso known as:Also known as:
Laboratory Fortified Blank (LFB)
Quality ControlSample (QCS)
-
8/6/2019 QA Course Total
25/32
SurrogatesSurrogates
Similar to an internal standard
Added to all analytical samples,
and to all QC samples to monitormethod performance, usually
during sample prep
Methods often have specific
surrogate recovery criteria
Most common in Organic methods
-
8/6/2019 QA Course Total
26/32
Quality Control MeasuresQuality Control Measures Standards and Calibration
BlanksRecovery Studies
Precision andAccuracy Studies
Method Detection Limits
NJQLs
-
8/6/2019 QA Course Total
27/32
Precision and AccuracyPrecision and Accuracy
Required for initial certification
and annually thereafter
A series of four laboratory control
samples
Must meet accuracy (recovery) andprecision (standard deviation)
requirements, often in method
-
8/6/2019 QA Course Total
28/32
Precision and AccuracyPrecision and AccuracyRequired with a change in
instrumentation or personnel Specific to the analyst
Other names include:
P&A, DOC, IDOC
-
8/6/2019 QA Course Total
29/32
Method Detection LimMethod Detection LimititThe minimum concentration of a
substance that can be measured andreported with 99% confidence that the
analyte concentration is greater than
zero
N.J.A.C 7:18N.J.A.C 7:18 -- 1.71.7
-
8/6/2019 QA Course Total
30/32
Method Detection LimitMethod Detection Limit MDLs are determined according to
40
CFR, part136
,Appendix B
Seven replicate laboratory control
samples, analyzed for precision
Multiply standard deviation by
3.14 (Students t- value)
-
8/6/2019 QA Course Total
31/32
Method Detection LimitMethod Detection LimitMust be performed initially for
certification
Must meet criteria specified inmethod
Must be performed with change in
instrumentation or test method
Annually with ELCP
-
8/6/2019 QA Course Total
32/32