Notes on Philippine Medically Important Protozoans Part 1 (Amoeba) Meccar Moniem Elino, BSBio IV , Davao Doctors College Instructor: Mrs. Chalee Sienes-Reyes, RMT, MSMT, Ph.D

Reference: Philippine textbook of Medical Parasitology 2nd ed.(2004) UP. Edited by Vicente Y. Belizario, Jr. and Winifreda U. de Leon

Cyst Tropohozoite

Size/Shape Cystic Wall No. of

Nucleus Cytoplasm

Chromatoidal Bars

Karyosome Size/Shape Cytoplasm Endoplasm Nucleus Chromatoidal

Bars karyosome

Entamoeba histolytica 5-20micra/spherical smooth, definite

1 to 4

rounded ends with glycogen

mass

central 10-

60micra/irr

grayish violet with

clear demarcation

vacuoles bluish black

with ingested rbc

single, spherical nucleus;

nucleus membrane is lined by fine,

regularly arranged

chromatin granules

small, center

Entamoeba coli 10-33micra/spherical smooth, definite

1 to 8

splintered ends with glycogen

mass

eccentric

15-50micra, condensed, thickened, rounded

mass

grayish violet; there is no clear

demarcation

highly vacuolated containing bacteria

spherical with thick nuclear membrane lined with

coarse

irregular chromatin granules

large eccentric

Endolimax nana 6-10micra/oval smooth, definite

1 to 4 darkly

stained

grayish granular

deeply stained

curved rods

6-15micra/irr

grayish violet

vacuole may contain bacteria

small/spherical largenucleus

large irregular

centric/eccentric

Entamoeba gingivalis x x x x x x 5-

35micra/irr

with clear demarcation of ecto and endo, same

pseudopodia direction of

E. histolytica

with bacteria and

rare rbc, spherical center

Iodamoeba butschlii 6 – 15 micra/irr smooth, definite

1 or rarely2

violet-gray granular

with large vacuole

unsained

absent or with stained small rods

6-

25micra/irr granular,

vacuolated

vacuoles may contain bacteria and

yeast cells

single /spherical

large

central/eccentric

Dientamoeba fragilis x x x x x x 3-

22micra/irr

granular, vacuolated

with bacteria

with 4-8 chromatin granules

2 with 4-8

chromatin granules