proteomics training kit - waters.com€¦ · proteomics training standards kit #2 (see table 2)...

[ CARE AND USE MANUAL ]

CONT ENTS

I. INT RODUCT ION

II. STANDARD P REPARAT ION

II I. P ROT EIN EX P RESSION: MASSP REP MIX 1 VS. MIX 2 (75 µm)

IV. P ROT EIN EX P RESSION: MASSP REP MIX 1 VS. MIX 2 (300 µm)

V. ORDERING INFORMAT ION

Proteomics Training Kit

I . INT RODUCT IONThe Proteomics Training Standards Kit is designed to provide

trainers and customers with high quality standards that can be

utilized as benchmark standards for performing qualitative /

quantitative proteomics experiments on their Waters® UPLC® inlet

combined with a time-of-flight (TOF) or Q-Tof mass spectrometry

detection systems. The training kit combines many of the individual

standard samples that are commonly used for instrument system

qualification in demonstrations and training classes, including

reference compounds for calibration and lockmass. This training

kit also includes stable isotope labeled (SIL) Hi3 peptide standards

(PhosB and ClbB) that provide an isotopically unique exogenous

standard to perform relative protein quantitation. These Hi3 SILAC

peptides can be used in several different cases, including: to

minimize interference with endogenous peptides, provide a second

level of internal quality control (i.e., spike Hi3 SILAC standards

toward the lower limit of detection), and perform relative protein

quantitation for mixed proteome samples. Overall, this training kit

provides the necessary foundation for LC-MS proteomic analysis

and can be used to evaluate system performance and optimize

methods prior to large-scale quantitative proteomics experiments.

There are two versions of the Proteomics Training Standard Kits

available. The Proteomics Training Standards #1 (See Table 1)

contains the necessary standards for LC-MS system performance

evaluation for complex proteomic digest samples. While the

Proteomics Training Standards Kit #2 (See Table 2) contains

standard reference peptides and proteins that are commonly used

for mass spectrometry instrument specifications and/or system

installation, such as [Glu1]-Fibrinopeptide B and bovine insulin.

Proteomics Training Standards Kit #2 is shipped at ambient

temperatures, however upon receipt of this kit, please store at

-20 ˚C to maximize long term stability.

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Table 1. Kit Components for Proteomics Training Kit #1 (p/n 186006918)

Part No. Contents Component Description Amount per Vial Container

186006918-1 1 vialHi3 Peptide Standard

E. coli~ 1 nmol per peptide

TruView™ Max Recovery with non-slit PTFE/silicone Septa (p/n 186005668CV)

186006918-2 1 vialHi3 Peptide Standard

Phos B~ 1 nmol per peptide

TruView Max Recovery with non-slit PTFE/silicone Septa (p/n 186005668CV)

186006918-3 1 x 30 mLSodium IodideCesium Iodide

2 µg/µL50 ng/µL

30 mL Clear Nalgene Bottle

186006918-4 1 vial Tryptic Digest of E. coli 100 µg/vialTruView Max Recovery with non-slit

PTFE/silicone Septa (p/n 186005668CV)

186006918-5 1 vial Digestion Standard Mix 1See Table 3 in

SAMPLE PREPARATIONTruView Max Recovery with non-slit


186006918-6 1 vial Digestion Standard Mix 2See Table 3 in

SAMPLE PREPARATIONTruView Max Recovery with non-slit


186006918-7 1 vial SILAC E. coli ~ 1 nmol per peptideTruView Max Recovery with non-slit


186006918-8 1 vial SILAC Phos B ~ 1 nmol per peptideTruView Max Recovery with non-slit


Table 2. Kit Components for Proteomics Training Kit #2 (p/n 186007528)

Part No. Contents Component Description Amount per Vial

186007528-1 1 vialLeucine enkephalin

acetate hydrate4.0 mg (± 0.08 mg)

186007528-2 1 vial [Glu1]-Fibrinopeptide B 1.0 mg (± 0.1 mg)

186007528-3 1 vial Bovine Insulin 3.0 mg (± 0.15 mg)

186007528-4 1 vial Horse Heart Myoglobin 3.4 mg (+1.0%, -0.0%)

I I . STANDARD P REPARAT ION

Section 1. Diluent Preparation

a. 97:3 water: acetonitrile + 0.1% trifluoroacetic acid (TFA)

1. Using a 100 mL graduated cylinder measure 30.0 mL of

LC-MS grade acetonitrile and add to a 1 L Waters Certified

Container (p/n 186007089).

2. Add LC-MS grade or Milli-Q water to the 900 mL mark.

3. Pour contents into a 1 L graduated cylinder and add LC-MS

grade or Milli-Q water to the 1.0 L mark.

4. Pour entire contents into the 1 L Waters Certified Container

and remove 1000 µL of solution using a 1000 µL pipette and

discard the solution.

5. Using caution, pour the contents of a 1.0 mL ampoule of

LC-MS grade 99.5% TFA.

6. Gently swirl the solution to mix.

7. Sonicate for 5 minutes.

8. Label as “97:3 H2O:ACN + 0.1% TFA”.

http://www.waters.com/waters/partDetail.htm?partNumber=186006918

http://www.waters.com/waters/partDetail.htm?partNumber=186005668CV









3



b. 75:25 water: acetonitrile + 0.1% formic acid

1. Using a 1 L graduated cylinder measure 250 mL of LC-MS

grade acetonitrile.

2. Add 750 mL of LC-MS grade or Milli-Q water and fill to the

1000 mL mark.

3. Transfer solution to a 1 L Waters Certified Container

(p/n 186007089).

4. Remove 1000 µL of solution using a 1000 µL pipette and

discard the solution.

5. Add 1000 µL of 99.9% LC-MS grade formic acid (FA) to

the 1 L bottle.

6. Gently swirl contents or vortex to mix.


8. Label as “75:25 H2O:ACN + 0.1% FA”.

c. 50:50 water: acetonitrile + 0.1% formic acid

1. Using a 100 mL graduated cylinder measure 50 mL

of LC-MS grade acetonitrile.


100 mL mark.

3. Transfer solution to a 250 mL bottle.

4. Remove 100 µL of solution using a 100 µL pipette and discard.

5. Add 100 µL of 99.9% LC-MS grade formic acid (FA) to the

250 mL bottle.



8. Label as “50:50 H2O:ACN + 0.1% FA”.

d. 50:50 water: acetonitrile + 0.2% formic acid

1. Using a 100 mL graduated cylinder measure 50 mL of LC-MS

grade acetonitrile.


100 mL mark.



5. Add 200 µL of 99.9% LC-MS grade formic acid (FA) to the

250 mL bottle.



8. Label as “50:50 H2O:ACN + 0.2% FA”

e. 50:50 water: methanol + 1.0% acetic acid

1. Using a 100 mL graduated cylinder measure 50 mL

of LC-MS grade methanol.

2. Add 50 mL of LC-MS grade or Milli-Q water and fill to

the 100 mL mark.


4. Remove 1000 µL of solution using a 1000 µL pipette

and discard.

5. Add 1000 µL of LC-MS Glacial acetic acid (Fluka p/n 49199) to

the 250 mL bottle.



8. Label as “50:50 H2O:MeOH + 1% Acetic Acid”.


4



Section 2. Sample Preparation

a. Leucine Enkephalin (200 pg/µL)

1. Using a 5000 µL pipette, add 5.0 mL of LC-MS grade or Milli-Q

water to the contents of the 4.0 mg (± 0.08 mg) bottle of

leucine enkephalin.

2. Recap, shake well, and sonicate for 5 minutes.

3. Label as “800 ng/µL leucine enkephalin in water”

and store in the freezer (expires in 3 months).

4. Using a 100 µL pipette, transfer 50 µL of the 800 ng/µL

leucine enkephalin in water to a 20-mL volumetric flask.

5. Make up to 20-mL with the “50:50 H2O:ACN + 0.1% FA”

solution and sonicate for 5 minutes.

6. Label as “2 ng/µL leucine enkephalin in 50:50 H2O:ACN +

0.1% FA” and store in the freezer (expires in 1 month).

7. For the 200 pg/µL leucine enkephalin solution remove 2000

µL of the 2 ng/µL leucine enkephalin solution and place in a

20-mL volumetric flask.

8. Make up to 20-mL with the “50:50 H2O:ACN + 0.1% FA”

solution and sonicate for 5 minutes.

9. Label as “200 pg/µL leucine enkephalin in 50:50 H2O:ACN +

0.1% FA” and store in the freezer (expires in 1 month).

10. For Fluidics systems, transfer from the 20-mL volumetric flask

to a 30-mL fluidics container and label as “200 pg/µL leucine

enkephalin in 50:50 H2O:ACN + 0.1% FA solution” and store in

the freezer (expires in 1 month).

b. [Glu1]-Fibrinopeptide B (100 fmol/µL)

1. Using a 1000 µL pipette, add 1000 µL of the 75:25 H2O:ACN

+ 0.1% FA solution to the contents of the 1.0 mg (± 0.1mg)

bottle of [Glu1]-Fibrinopeptide B (GFP).


3. Label as “1 mg/mL (637 pmol/µL) GFP in 75:25 H2O:ACN +

0.1% FA” and store in the freezer (expires in 3 months).

4. Using a 500-mL graduated cylinder, add 500-mL of the 75:25

H2O:ACN + 0.1% FA solution to a 500-mL Waters Certified

Container (p/n 186007090).

5. Remove 78.5 µL of solution and discard.

6. Using a 100 µL pipette, transfer 78.5 µL of the 1 mg/mL

(637 pmol/µL) GFP in 75:25 H2O:ACN + 0.1% FA solution

to a 500-mL Waters Certified Container (p/n 186007090).

7. Label as “100 fmol/µL GFP in 75:25 H2O:ACN + 0.1% FA” and

store at room temperature for use as lockmass solution.

8. Note that adding leucine enkephalin (100 fmol/µL) is

recommended to perform detector voltage check and mass

calibration cross validation using GFP as the TOF calibrant.

c. Bovine Insulin (5 pmol/µL)

1. Using a 5000 µL pipette, add 5250 µL of LC-MS grade

methanol and add 5250 µL of LC-MS grade or MilliQ water to

the contents of a 3 mg (±0.15 mg) bottle of bovine insulin and

sonicate for 5 minutes.

2. Label as “50 pmol/µL bovine insulin solution” and store in the

refridgerator (expires in 3 months).

3. Using a 1000 µL pipette, transfer 2000 µL of the 50 pmol/µL

bovine insulin solution to a 20-mL volumetric flask.

4. Make up to 20 mL with 50:50 water:methanol + 1% acetic acid

and sonicate for 5 minutes.

5. Label as “5 pmol/µL bovine insulin soluion in 1% acetic acid”

and store in the refridgerator (expires in 1 month).

6. For Fluidics systems, transfer from the 20-mL volumetric

flask to a 30-mL fluidics container and label as “5 pmol/µL

bovine insulin solution in 1% acetic acid” and store in the

refridgerator (expires 1 week).

7. For LC-MS experiments, the 5 pmol/µL bovine insulin solution

will need to be diluted 10-fold in LC-MS grade or MilliQ water

to result in a 500 fmol/µL bovine insulin in a 95:5 H2O:MeOH

+ 0.1% acetic acid solution.

8. Inject 1–5 µL for LC-MS analysis of the intact protein,

depending on the sensitivity of the instrument.



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d. Horse Heart Myoglobin Solution (200 fmol/µL)

1. Using a 5000 µL pipette, add 2000 µL of 50:50 H2O:

ACN + 0.2% FA solution to the contents of the 3.4 mg

(+1.0%, -0.0%) bottle of horse heart myoglobin.


3. Label as “100 pmol/µL horse heart myoglobin solution”

and store in the freezer (expires 1 month).

4. Using a 5000 µL pipette, transfer 20 mL of 50:50 H2O:

ACN + 0.2% FA solution into a 30-mL fluidics container.


6. Using a 100 µL pipette, add 40 µL of the 100 pmol/µL horse

heart myoglobin solution to result in a 200 fmol/µL horse

heart myoglobin solution.


8. Label as “200 fmol /µL horse heart myoglobin solution”

and store in the freezer (expires 1 month).

9. The 200 fmol/µL horse heart myoglobin solution will be used

for direct infusion experiments using the standard ESI or

nanospray ion sources.

10. For LC-MS experiments, the 100 pmol/µL myoglobin solution

will need to be diluted 10-fold in LC-MS grade or MilliQ water

to result in a 10 pmol/µL horse heart myoglobin in a 95:5

H2O:ACN + 0.02% FA solution.

11. Inject 1–10 µL for LC-MS analysis of the intact protein,

depending on the sensitivity of the instrument.

e. Hi3 Peptide Standard E. coli / Phos B (1 pmol/µL)

1. Take each vial of Hi3 E. coli ClpB / Rabbit PhosB (1 nmol/vial)

and using a 1000 µL pipette, add 1000 µL of 97:3 H2O:

ACN + 0.1% TFA.

2. Vortex and sonicate for 5 minutes. Stock concentration is

1 pmol/µL.

3. Label each vial as “1 pmol/µL Hi3 E. coli ClpB and 1 pmol/µL

Hi3 Rabbit PhosB in 97:3 H2O:ACN + 0.1% TFA*”, respectively.

4. The 1 pmol/µL stock solutions can be diluted to the desired

concentration depending on the application and column inner

diameter (i.e., 75 µm vs. 300 µm chromatography).

*Note that the solution is currently for 1D nanoACQUITY® with

trapping. If using a 2D RP/RP setup, change the solution to

20 mM ammonium formate pH 10.

f. SILAC Hi3 Peptide Standard E. coli / PhosB

1. Take each vial of SILAC Hi3 E. coli ClpB / Rabbit PhosB

(1 nmol/vial) and using a 1000 µL pipette, add 1000 µL

of 97:3 H2O:ACN + 0.1% TFA.

2. Vortex and sonicate for 5 minutes. Stock concentration

is 1 pmol/µL.

3. Label each vial as “1 pmol/µL SILAC Hi3 E. coli ClpB

and 1 pmol/µL Rabbit PhosB in 97:3 H2O:ACN +

0.1% TFA*”, respectively.

4. The 1 pmol/µL stock solutions can be diluted to the desired

concentration depending on the application and column inner

diameter (i.e., 75 µm vs. 300 µm chromatography).

*Note that the solution is currently for 1D nanoACQUITY with

trapping. If using a 2D RP/RP setup, change the solution to

20 mM ammonium formate pH 10.

g. Digestion Standard Mix 1

1. Take the vial of MassPREP™ Mixture 1 and using a 1000 µL

pipette, add 1000 µL of the 97:3 H2O:ACN + 0.1% TFA

solution. Table 1 shows the respective pmol amounts of each

protein in the vial.

2. Vortex and sonicate for 5 minutes. The stock concentrations are

shown in Table 4.

3. Label as “Mixture 1 stock solution”.

4. Refer to E. coli digest Mix 1 / Mix 2 sample preparation section

for dilution instructions.

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h. Digestion Standard Mix 2

1. Take the vial of MassPREP Mixture 2 and using a 1000 µL

pipette, add 1000 µL of the 97:3 H2O:ACN + 0.1% TFA solution.

2. Vortex and sonicate for 5 minutes. Table 4 shows the stock

concentrations of both Mix 1 and Mix 2.

3. Label as “Mixture 2 stock solution”.

4. Refer to E. coli digest Mix 1 / Mix 2 sample preparation section

for dilution instructions.

Table 3. Vial Contents of MDPS Mix 1 and Mix 2

protein digestion standards.

ProteinMDPS Mix 1 [Molar ratio,

Amount (pmol)]

MDPS Mix 2 [Molar ratio,

Amount (pmol)]

ADH 1.0, 50 pmol 1.0, 50 pmol

GPB 1.0, 50 pmol 0.5, 25 pmol

ENO 1.0, 50 pmol 2.0, 100 pmol

BSA 1.0, 50 pmol 8.0, 400 pmol

Table 4. Stock solution concentrations of MassPREP Mix 1

and Mix 2 protein digestion standards.


Amount (pmol)]


Amount (pmol)]

ADH 1.0, 50 fmol 1.0, 50 fmol

GPB 1.0, 50 fmol 0.5, 25 fmol

ENO 1.0, 50 fmol 2.0, 100 fmol

BSA 1.0, 50 fmol 8.0, 400 fmol

I I I . P ROT EIN EX P RESSION: MASSP REP MIX 1 VS. MIX 2 (75 µm)The following is an example of the E. coli Mix 1 vs E. coli Mix 2 protein

expression experiment ran on a Waters SYNAPT® G2-Si HDMS® Mass

Spectrometer using 75 µm scale chromatography.

Section 1. Sample Preparation1. Take the vial of lyophilized E. coli digest (100 µg/vial) and

using a 100 µL pipette, add 100 µL of the 97:3 H2O:ACN +

0.1% TFA solution.

2. Label as “1 µg/µL E. coli stock solution”.

3. In a TruView LC-MS Certified Total Recovery vial with a

pre-slit PTFE/silicone septa (p/n 186005669CV) add the

following solutions and volumes defined in Table 5.

4. Table 6 shows the resulting concentrations of the MassPREP

Mix 1 / Mix 2 proteins and their expected molar ratios.

Table 5. Sample preparation details for E. coli Mix 1 / E. coli

Mix 2 for 75 µm scale chromatography. *Note that the SILAC Hi3

standards are added for internal quality control (QC) standard.

Adding these SILAC standards are optional.

E. coli Mix 1 Solution

Volume (µL)

E. coli Mix 2 Solution

Volume (µL)

E. coli stock digest (1 µg/µL)

10E. coli stock

digest (1 µg/µL)10

Mix 1 stock 20 Mix 2 stock 20

*SILAC Hi3 E. coli ClpB10 fmol/µL

10*SILAC Hi3 E. coli ClpB 10 fmol/µL

10

*SILAC Hi3 PhosB

50 fmol/µL10

*SILAC Hi3 PhosB

50 fmol/µL10

97:3 H2O:ACN + 0.1% TFA

5097:3 H2O:ACN

+ 0.1% TFA50

Table 6. Resulting concentrations of the MassPREP Mix 1 and Mix

2 proteins after dilution into the E. coli lysate background.


Amount (pmol)]


Amount (pmol)]

ADH 1.0, 10 fmol 1.0, 10 fmol

GPB 1.0, 10 fmol 0.5, 5 fmol

ENO 1.0, 10 fmol 2.0, 20 fmol

BSA 1.0, 10 fmol 8.0, 80 fmol

7



Section 2. LC-MS/MS (75 µm) Experimental ConditionsConditions:

Inlet: nanoACQUITY System ran in a single pump

trapping mode

Solvent A: 0.1% formic acid in water

Solvent B: 0.1% formic acid in acetonitrile

Weak wash: 97:3 H2O:ACN + 0.1% TFA

Trapping: 10 µL/min for 3 min, 10 µL loop

Column: ACQUITY ULC M-Class HSS T3 Column,

1.8 µm, 75 µm x 150 mm, 1/pkg

(p/n 186007473)

Trap: ACQUITY UPLC M-Class Symmetry C18

Trap Column, 100A, 5 um, 180 um x 20 mm,

2G, V/M 1/pkg (p/n 186007496)

Gradient profile:

Time Flow Rate

(min) (µL/min) %A %B Curve

0.00 0.500 95.0 5.0 6

54.00 0.500 60.0 40.0 6

55.80 0.500 15.0 85.0 6

59.40 0.500 15.0 85.0 6

61.20 0.500 95.0 5.0 6

72.00 0.500 95.0 5.0 6

Figure 1. Base Peak Intensity (BPI) chromatograms: (A) E. coli Mix 1 and (B) E. coli Mix 2 ran using a 75 µm x 150 mm column. MS conditions: MSE using alternate scanning low (4 V) and elevated energy (15–45 V) and a scan time of 0.5 seconds. Injection volume: 1.0 µL of the sample for each condition.

Figure 2. Progenesis® QI for Proteomics: Data was processed for the quintuplicate injections of E. coli Mix 1 and E. coli Mix 2. The image illustrates the Protein Statistics data review with a Principal Component Analysis showing each injection for the relative expression levels of ALBU_BOVIN (expected expression level increase 8-fold).

Figure 3. Protein expression comparison of the relative fmol amounts of each MassPREP Mix 1 and Mix 2 standard protein (75 µm chromatography). Note that the average fmol amounts are shown with the respective standard deviation error bars.

IV. P ROT EIN EX P RESSION: MASSP REP MIX 1 VS. MIX 2 (300 µm)The following is an example of the E. coli Mix 1 vs E. coli Mix 2 protein

expression experiment ran on a Waters SYNAPT G2-Si HDMS Mass

Spectrometer using 300 µm scale chromatography. The ACQUITY

UPLC® 2D M-Class HCP System was used for these experiments.



8



Section 1. Sample Preparation1. Take the MassPREP Mixture 1 / Mixture 2 stock solutions

prepared earlier and dilute both Mix 1 / Mix 2 according to

the following procedure.

2. Using a 100 µL pipette, add 200 µL of Mix 1 and Mix 2 and

place the volume in two individual TruView Max Recovery vials

and label as “Mix 1 and Mix 2”, respectively.

3. Using a 10 µL pipette, add 1 µL of the 1 pmol/µL SILAC E. coli

ClpB to each Mix 1 and Mix 2 sample vials.

4. Using a 10 µL pipette, add 5 µL of the 1 pmol/µL SILAC Rabbit

PhosB to each Mix 1 and Mix 2 sample vials.

5. Using a 1000 µL pipette, add 794 µL of 20 mM ammonium

formate pH 10 to each Mix 1 and Mix 2 sample vials.

6. Cap each vial and vortex.

7. Label these solutions as “Mix 1 Diluted Solution” and “Mix 2

Diluted Solution”. These solutions will be used to resuspend

lyophilized aliquots of E. coli digest. Table 7 contains the

diluted concentrations of Mix 1 and Mix 2.

8. Using a 100 µL pipette, add 100 µL of “Mix 1 Diluted Solution”

to a vial of E. coli digest (100 µg). Cap the vial and Vortex.

9. Using an additional vial of E. coli digest (100 µg), add 100 µL of

“Mix 2 Diluted Solution”. Cap the vial and Vortex. Note that for

the 300 µm or 1 mm scale chromatography it is recommended

that a least two vials of E. coli digest be used for the E. coli

Mix 1 vs. E. coli Mix 2 experiment.

Table 7. Diluted concentrations of MDPS Mix 1 and Mix 2 protein

digestion standards for 300 µm chromatography.


Amount (pmol)]


Amount (pmol)]

ADH 1.0, 10 fmol 1.0, 10 fmol

GPB 1.0, 10 fmol 0.5, 5 fmol

ENO 1.0, 10 fmol 2.0, 20 fmol

BSA 1.0, 10 fmol 8.0, 80 fmol

Section 2. LC-MS/MS (300 µm) Experimental ConditionsConditions:

Inlet: 2D ACQUITY UPLC M-Class HCP System ran

in simulated 1D mode

Solvent A: 0.1% formic acid in water

Solvent B: 0.1% formic acid in acetonitrile

Trapping: Simulated 1D (elute from 1st dimension with

50% B) 10 µL/min for 26 min, 100 µL loop

Column: 1st dimension: XBridge® C18, 2.5 µm,

1.0 x 50 mm (p/n 186003118)

2nd dimension: ACQUITY UPLC M-Class HSS T3 Column,

1.8 μm, 300 μm x 150 mm (p/n 186007472)

Trap: ACQUITY UPLC M-Class Symmetry C18 Trap

Column, 100A, 5 μm, 300 μm x 25 mm,

HCP (2D) (p/n 186007499)

Gradient profile:

Time Flow Rate

(min) (µL/min) %A %B Curve

0.00 10.0 97.0 3.0 6

63.00 10.0 65.0 35.0 6

70.00 10.0 50.0 50.0 6

72.00 10.0 10.0 90.0 6

77.00 10.0 97.0 90.0 6

79.00 10.0 97.0 3.0 6

90.00 10.0 97.0 3.0 6

Figure 4. Base Peak Intensity (BPI) chromatograms: (A) E. coli Mix 1 and (B) E. coli Mix 2 ran using a 300 µm x 150 mm column. MS conditions: MSE using alternate scanning low (4 V) and elevated energy (15–45 V) and a scan time of 0.5 seconds. Injection volume: 2.0 µL of the sample for each condition.




9



Figure 5. Progenesis® QI for Proteomics: Data was processed for the quintuplicate injections of E. coli Mix 1 and E. coli Mix 2. The image illustrates the Review Proteins data review section with a list of the protein identifications and the relative fold change amounts and Hi3 relative quantitative fmol amounts for each protein identification. The highlighted protein, ENO1_YEAST, shows an increase in the expression level for E. coli Mix 2 sample (expected expression level increase 2-fold).

Figure 6. Protein expression comparison of the relative fmol amounts of each MDPS Mix 1 and Mix 2 standard protein (300 µm chromatography). Note that the average fmol amounts are shown with the respective standard deviation error bars.

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com


Waters, The Science of What’s Possible, ACQUITY UPLC, Xevo, SYNAPT, nanoACQUITY, and Progenesis are registered trademarks of Waters Corporation. MassPREP, and TruView are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2015 Waters Corporation. Printed/Produced in the U.S.A. September 2015 720005511EN IH-PDF

V. ORDERING INFORMAT ION

Proteomics Training Standards #1 (p/n 186006918 )

Includes the following p/n’s to be stored at room temperature:


186006012 1 vial Hi3 Peptide Standard E. coli ~ 1 nmol per peptide

186006011 1 vial Hi3 Peptide Standard Phos B ~ 1 nmol per peptide

186006918-3* 1 x 30 mLSodium Iodide Cesium Iodide

2 µg/µL 50 ng/µL

186003196 1 vial Tryptic Digest of E. coli 100 µg/vial

186002865 1 vial Digestion Standard Mix 1 ~ 1 nmol

186002866 1 vial Digestion Standard Mix 2 ~ 1 nmol

186007084 1 vial SILAC E. coli ~ 1 nmol per peptide

186007083 1 vial SILAC Phos B ~ 1 nmol per peptide

Proteomics Training Standards #2 (p/n 186007528)

Includes the following p/n’s to be stored at -20 °C ± 10 °C (frozen) immediately upon arrival:


176007528-1* 1 vial Leaucine Enkephalin 4.0 mg

176007528-2* 1 vial [Glu1]-Fibrinopeptide B 1.0 mg

176007528-3* 1 vial Bovine Insulin 3.0 mg

176007528-4* 1 vial Horse Heart Myoglobin 3.4 mg

*Not sold separately outside of the kit.



proteomics training kit - waters.com€¦ · proteomics training standards kit #2 (see table 2)...

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