protein synthesis. transcription dna mrna occurs in the nucleus translation mrna trna aa occurs...
TRANSCRIPT
Protein Synthesis
Protein Synthesis
Protein Synthesis
TranscriptionDNA mRNA
Occurs in the nucleus
TranslationmRNA tRNA AA
Occurs at the ribosome
RNA and DNA Compared
Triplets (Codons)
Genetic Code
Transcription
InitiationPromoter
TATA BoxInitiation Site
RNA PolymeraseAUG
Elongation
RNA Polymerase
Adds nucleotides to the 3’ end
Termination
Proceeds until RNA polymerase transcribes a terminator sequence
UAA
UAG
UGA
RNA Processing
5’ guanine cap
3’ Poly (A) tail
RNA Splicing
Introns
Exons
Introns????
Regulation
One gene can code for alternate polypeptides
EvolutionDomains – structural and functional regions
Translation
tRNA
Aminoacyl-tRNA
Synthetase
Ribosomes
Small subunit
Large subunitP site (peptidyl-tRNA)
A site (Aminoacyl-tRNA)
E site (Exit site)
Transcription
Initiation
The first tRNA with its AA attaches to the mRNA and the small subunit
The first tRNA and its AA enters the P site
Elongation
The second tRNA and its AA enters the A site
A peptide bond forms
Elongation
Translocation
The third tRNA and its AA enters the A site
A peptide bond forms
Termination
Polyribosomes
Point Mutation
Point Mutation
Base Pair Substitution
Frame ShiftMutation
Base Pair Insertion or Deletion
