protein metrics solutions
TRANSCRIPT
Bispecific Antibodies
Bispecific AntibodyPossess the characteristics and specificity
of two distinct monoclonal antibodies
What is a Bispecific Antibody?
Dillon & Yin (2016) MABS 9: 213-230Schachner & Han (2016) Anal. Chem. 88: 12122-12127
RECRUIT cytotoxicT-cells or NK-cells
ACTIVATE receptor
• Possess the characteristics and specificity of two distinct monoclonal
antibodies
• Can bind to two different targets simultaneously
• >50 molecules currently in clinical development
• Complex multi-chain molecules that can be challenging to produce in
sufficient quantity and quality
• Bispecific antibody formats based on IgG preferred for many applications
• Easy purification
• Long serum half-life
• Effector functions
Wilson Phung, Genentech (Webcast Sept 2017)(https://www.technologynetworks.com/tn/webinars/high-throughput-quantitative-analysis-of-bispecific-antibody-moieties-and-glycosylation-290703)
What is a Bispecific Antibody?
Dillon & Yin (2016) MABS 9: 213-230Schachner & Han (2016) Anal. Chem. 88: 12122-12127
DELIVERY acrossblood brain barrier
BLOCK two independentligands or receptors
• Possess the characteristics and specificity of two distinct monoclonal
antibodies
– Can bind to two different targets simultaneously
• >50 molecules currently in clinical development
• Complex multi-chain molecules that can be challenging to produce in
sufficient quantity and quality
• Bispecific antibody formats based on IgG preferred for many applications
– Easy purification
– Long serum half-life
– Effector functions
Wilson Phung, Genentech (Webcast Sept 2017)(https://www.technologynetworks.com/tn/webinars/high-throughput-quantitative-analysis-of-bispecific-antibody-moieties-and-glycosylation-290703)
Alternative Formats for Bispecific Antibodies
Technical Hurdles to Bispecific Production
+ + +
Single Cell Production – in vivo assembly
Unwanted IgG speciesDesired Bispecific
Problems to solve:
1. Unwanted heavy chain homodimerization
2. Unwanted light chain – heavy chain pairing
Bi-Specific webinar with Genentech
• Moving data processing to the next level of efficiency
• Software to quickly identify and report desired, undesired and unknown species
• High throughput capabilities
https://www.technologynetworks.com/tn/webinars/high-throughput-quantitative-analysis-of-bispecific-antibody-moieties-and-glycosylation-290703
Workflow Automation - Bispecifics
Identification of desired forms
INCORRECT
Identification of undesired forms
Antibody Drug Conjugates (ADC)
ADCs
Drug loading distribution (by sample)
DAR values (Automatically calculated)
ADC webinar StemCentrx (BRK and PMI)
• Ability to use ALL of the data, no matter how complex
• Bruker data on Antibody-Drug-Conjugates (ADCs) in a StemCentrx webinar shows complex data
• Need to calculate ‘Drug-to-Antibody Ratio’ (DAR)
• StemCentrx, Bruker and Protein Metrics worked to improve the workflow in a real-world environment with HIGHLY COMPLEX data
http://worldadc-webinars.com/view-webinars/webinar-archive/
