prospect of endophytic fungi for jathropha curcas plantation on post mining area
TRANSCRIPT
Prospect of endophytic fungi for
Jatropha curcas plantation on post
mining area
Utut Widyastuti, Hamim, Nampiah Sukarno, Sukma Gideon
Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB
Ecological damage on post tin mining activity
How are we save our soil to become more sustainable?
RevegetationJatropha curcas : can survive on marginal land;
Used for biofuel
Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB
Visi on 2010 (organic agriculture)
Biofretilizer:
Root mutualistic fungi
Biofertilizer from Outside country
Problem :Non-indigenous microbe
Indonesia
Rich on genetic diversity
Indigenous biofertilizer
Aspergillus sp
Control Aspergillus niger applicationwithout shaking
Aspergillus niger application with shaking
Enhanced germination of Jatropha curcasby Aspergillus niger
Treatment % Germinate
Dormant break down by heating
Aspergillus niger Filtrate 48,57
Control 30, 00
Non dormant break down
Aspergillus niger filtrate
31,43
Control 10,00
Enhanced germination of Jatropha curcasby Aspergillus niger
a. Control
A green house study
With lower concentrationof Phosfat
b. Aspergillus sp c. CMA
Kustiarini, 2003
How are Aspergillus sp effect for growing of Jatropha curcas on mining
activity soil ?
RevegetationJatropha curcas : can survive on marginal land;
Used for biofuel
Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB
Tin mining activity soils in P. Bangka-Belitung
Low pH < 4Heavy metal
Increased of growth jatropha curcas on tin mining activity soil with endophytic fungi
Control
5 month in tin mining area
5 month
1 month
Aspergillus niger treatment (250 g/hole)
Effect of endophytic fungi doses to growth Jatropha curcas on tin mining activity soil
25
26
27
28
29
30
31
32
33
34
35
1 2
Series1
Doses
500 g/hole 250 g/hole
Heigh
t (cm)
25
35
0
0.5
1
1.5
2
2.5
3
3.5
4
1 2
Series1
500 g/hole 250 g/hole
Num
ber of b
ranching
Doses
Performance of Jatropha curcas on tin mining area with endophytic fungi
10 month
Enhanced of growth Jatropha curcas in post tin mining area with combination of endophytic fungi, top soil and compost
25
26
27
28
29
30
31
32
33
34
35
1 2
Series1
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
1 2
Series1
Heigh
t (cm)
Num
ber of b
ranching
Aspergillus sp Aspergillus spCombination Combination
Enhanced of growth Jatropha curcaswith combination: endophytic fungi, top soil, compost
10 month
Conclusion
Treatment with Aspergillus niger as a biofertilizer provided nutriens to support plant growth
Endophytic fungi was needed for establishment ofJatropha curcas growth on post tin mining area.
Research Center for Bioresourches and Biotechnology –IPB; Unit for comercial services and industry (UJI) Department Biology-FMIPA IPB
Acknowledgement