Scientific Program Neutrophil 2018 International SymposiumHôtel Château Laurier, Québec, Qc, Canada

15h00-18h00 On-site registration begins19h00 Introductory remarks Co-chairs (P McDonald, PE Poubelle)19h15-20h00 Keynote Lecture: Dr Paul Kubes (University of Calgary, Canada)

Live fast, die young, and leave a good-looking corpse

20h15 Welcoming Reception and Cocktail (Des Plaines Ballroom or Jardin des Quatre saisons Courtyard, weather permitting)

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Saturday, June 2

Neutrophil 2018Québec City, June 2-5 2018

8h00 Continental breakfast (Des Plaines Ballroom)

Session I: Classic Neutrophil FunctionsChairs: SG Bourgoin, M Pouliot, OE Sørensen

8h30- 9h00 Ole E. Sørensen (Lunds Universitet, Lund, Sweden)A life in science devoted to neutrophil granules (In memoriam, Dr Niels Borregaard)

9h00- 9h30 Anthony W. Segal (University College London, London, UK)Vacuolar pH, its regulation and role in microbial killing

9h30- 10h00 Anna Huttenlocher (University of Wisconsin, Madison, USA)Reverse neutrophil migration and resolution of inflammation

10h00- 10h30 Hongbo R. Luo (Harvard Medical School, Boston, USA)Regulation of neutrophil function by inositol hexakisphosphate kinase 1 (IP6K1)

10h30-10h45 Coffee break10h45-11h15 Edwin R. Chilvers (University of Cambridge, UK)

Neutrophil priming: a one way ticket?

Selected talks from abstracts 11h15- 11h30 Jennifer C. Brazil (University of Michigan, USA)

N-linked glycans on CD11b/CD18 regulate PMN transepithelial migration

11h30- 11h45 Sergio D. Catz (The Scripps Research Institute, USA)JFC1 regulates Rac1-GTP uropod localization to control neutrophil directional sensing, chemotaxis and in vivo migration

Poster session11h45-12h30 Poster rooms12h30-13h30 Lunch

Session II: Neutrophil Extracellular TrapsChairs: J-P Lavoie, PP McDonald, DD Wagner

13h30- 14h00 Denisa D. Wagner (Harvard Medical School, Boston, USA)NETs: nuclear weapons of inflammation

14h00- 14h30 Johan van der Vlag (Radboud University, Nijmegen, The Netherlands)The ins and outs of NETs

14h30- 15h00 Balázs Rada (University of Georgia, Athens, GA, USA)Neutrophil extracellular traps in cystic fibrosis airway disease

15h00- 15h30 Martin Herrmann (Friedrich-Alexander-Universität, Erlangen, Germany)Neutrophils, NETs and aggNETs contribute to initiation and resolution of inflammation

15h30-15h45 Coffee break15h45- 16h15 Discussion/workshop on NETs

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Sunday, June 3

Trainees’ Session : Part 1Chairs: Sarah Walmsley, Marc Pouliot 16h15- 16h27 Ania Bogoslowski (University of Calgary, Canada)

Reconnaissance neutrophils survey lymph nodes at steady state

16h27-16h39 Laurence S.C. Lok (Cambridge University Hospitals, UK)The Homeostatic role of lymph node-resident neutrophils in adaptive immunity

16h39-16h51 Immanuel Kwok (Singapore Immunology Network)Developmental analysis of bone marrow neutrophils reveals populations specialized in expansion, trafficking and effector functions

16h51-17h03 Brian Hsu (McGill University, Montréal, Canada)Immature neutrophils promote breast cancer liver metastasis by suppressing NK cell-mediated immunosurveillance

Poster session17h20- 18h20 Poster rooms

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8h00 Continental breakfast (Des Plaines Ballroom)Session III: Emerging Topics in Neutrophil BiologyChairs: M Fernandes, T Lämmerman, S Renshaw 8h30 - 9h00 Tim Lämmerman (Max-Planck-Institut, Freiburg, Germany)

Neutrophil swarming at sites of inflammation and infection 9h00 - 9h30 Sarah Walmsley (University of Edinbugh, UK)

Hypoxia, metabolism and neutrophilic inflammation 9h30 - 10h00 Venizelos Papayanopoulos (The Francis Crick Institute, London, UK)

Neutrophils in the regulation of inflammationSelected talks from abstracts 10h00- 10h15 Helen L. Wright (University of Liverpool, UK)

Proteomic analysis of neutrophil extracellular traps10h15- 10h30 Mihaela Gadjeva (Harvard Medical School, Boston, USA)

Neutrophil maturation and their response to infectious pathogens are regulated by microbiota

10h30 -10h45 Coffee break

Session IV: Plenary WorkshopsNOMENCLATURE OF NEUTROPHIL SUBSETS / NEW METHODOLOGIESChairs: M. Glogauer, J-P Lavoie, PE Poubelle10h45 - 11h00 Michael Glogauer (University of Toronto, Canada)

A pro-inflammatory first responding neutrophil activation subset is augmented during emergency granulopoiesis and neutropenia

11h00 - 11h15 Steven W. Edwards (University of Liverpool, UK)Genetic reprogramming in human neutrophils during inflammation

11h15 - 11h30 Leo Koenderman (University Medical Center Utrecht, The Netherlands)Kinetics of human neutrophil subsets in blood and bone marrow

11h30 - 11h45 Patrizia Scapini (Università di Verona, Italy)Neutrophil heterogeneity in acute and chronic inflammatory conditions

11h45 - 12h00 Daniel Irimia (Harvard Medical School, Boston, USA) Microscale tools for probing neutrophil sepsis, swarming, and interactions with microbes

12h00 - 12h45 Discussion on Neutrophil subsets / New methodologies

12h45 - 14h00 Lunch

Trainees’ Session : Part 2Chairs: S Walmsley, M Pouliot

14h00 -14h12 Maximilian Mauler (Heart Center, University of Freiburg, Germany)Neutrophil transmigration during myocardial reperfusion injury is boosted by platelet serotonin

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Monday, June 4

Trainees’ Session Part 2 (continued)14h12 - 14h24 Coraline Radermecker (GIGA-R, University of Liège, Belgium)

Neutrophil extracellular traps as major determinants of asthma

14h24 - 14h36 Hannah Isles (University of Sheffield, UK)Regulation of inflammation resolution by differential neutrophil migration patterns in zebrafish

14h36 - 14h48 Camilia Margaroli (Emory University, Atlanta, USA)Transcriptional burst promotes functional adaptation in neutrophils recruited to cystic fibrosis airways

Home-grown scienceChairs: PP McDonald, PE Poubelle

15:00-15:30 Martin Pelletier (Université Laval, Québec, Canada)Targeting neutrophil metabolism to control inflammatory responses

15:30-16:00 Nicolas Flamand (Université Laval, Québec, Canada)Neutrophils and the cannabinoid system: crosstalk with eicosanoids

16h00-16h20 Coffee break

Session V: Neutrophils in Adaptive Immunity and BeyondChairs: M Fernandes, J Filep, MC Karlsson

16h20-16h50 Mikael C. Karlsson (Karolinska Institutet, Stockholm, Sweden)Neutrophil regulation of autoreactive B cell responses

16h50-17h20 Luc Vallières (Université Laval, Québec, Canada)Pervasive effects of neutrophils in B cell-dependent autoimmune demyelination

17h20-17h50 Marco A. Cassatella (Università di Verona, Verona, Italy)Advances on the cytokine production and gene expression regulation in human neutrophils activated via TLR8

Selected talks from abstracts 17h50-18h05 Jyotika Sharma (University of North Dakota, Grand Forks, USA)

Klebsiella pneumoniae infection of neutrophils inhibits their efferocytic clearance by modulating cell death pathway

18h05-18h20 Gregory L. Kabachinski (AbbVie)NETosis signaling is not conserved across species

18h20 Cocktails and Poster Awards (Des Plaines Ballroom)or Jardin des Quatre saisons Courtyard (weather permitting)

19h30 Pub Dinner at Les Trois Brasseurs (optional)

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8h00 Continental breakfast (Des Plaines Ballroom)

Session VI: Neutrophils in Autoimmune Disease and CancerChairs: PE Poubelle, O Söhnlein

8h30- 9h00 Oliver Söhnlein (Klinikum der Universität München, Munich, Germany)Kiss of death: neutrophils kill smooth muscle cells to induce atherosclerotic plaque destabilization

9h00- 9h30 Mariana J. Kaplan (National Institutes of Health, Bethesda, USA) Neutrophils and their death in the pathogenesis of systemic autoimmune diseases

9h30- 10h00 Peter A. Nigrovic (Harvard Medical School, Boston, USA) Modulation of neutrophil and megakaryocyte function by emperipolesis

10h00- 10h30 Zvi Fridlender (Hadassah Medical Center, Jerusalem, Israel)Different sub-populations of cancer-related neutrophils, their activation and recruitment into tumors

10h30- 11h00 Stephen Renshaw (University of Sheffield, UK)Reverse migration as a therapeutic target in inflammation resolution

Selected talks from abstracts11h00-11h15 Sven Brandau (University Hospital Essen, Germany)

Identity of low-density neutrophils in cancer and their relation to MDSCs

11h15-11h30 Karin Christenson (University of Gothenburg, Sweden)Neutrophils in inflamed joints retain phenotypic characteristics of naïve blood cells

Concluding remarks11h30-11h45 PP McDonald, PE Poubelle, S Renshaw

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Tuesday, June 5