preparation of competent e. coli cells transformation of the ligated construct into e. coli...
TRANSCRIPT
Preparation of competent E. coli cells
Transformation of the ligated construct into E. coli
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322A
Selection of recombinant E. coli cells
Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite
Digestion of pBR322BamH1 with BamH1 and EcoRI restrictionenzymes
Quality control by electrophoreses
Digestion of pBR322 with BamH1
Digestion of pBR322 with BamH1
Elongation of BamH1 restrictionsites, making of blunt ends
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322
Preparation of competent E. coli cells
Transformation of the ligated construct into E. coli
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322A
Preparation of competent E. coli cells
Transformation of the ligated construct into E. coli
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322A
Selection of recombinant E. coli cells
Preparation of competent E. coli cells
Transformation of the ligated construct into E. coli
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322A
Selection of recombinant E. coli cells
Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite
Preparation of competent E. coli cells
Transformation of the ligated construct into E. coli
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322A
Selection of recombinant E. coli cells
Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite
Digestion of pBR322BamH1 with BamH1 and EcoRI restrictionenzymes
Preparation of competent E. coli cells
Transformation of the ligated construct into E. coli
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322A
Selection of recombinant E. coli cells
Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite
Digestion of pBR322BamH1 with BamH1 and EcoRI restrictionenzymes
Quality control by electrophoreses
Preparation of competent E. coli cells
Transformation of the ligated construct into E. coli
Purification of the digested fragment
Purification of the pBR322BamH1 plasmid
Digestion of pBR322 with BamH1
Elongation of BamH1
restrictionsites,
making of blunt ends
Ligation of the blunt end in pBR322A
Selection of recombinant E. coli cells
Isolation and purifictation of the pBR322 plasmid without BamH1 restrictionsite
Digestion of pBR322BamH1 with BamH1 and EcoRI restrictionenzymes
Quality control by electrophoreses