polyphenols and aging
DESCRIPTION
William Kim Science Research – Period 4 Mrs. Flanagan. Polyphenols and Aging. Need. Figure 1: Number of people age 65 and over, by age group, selected years 1900-2006 and projected 2010-2050. Need. Figure 2: Life expectancy at ages 65 and 85, by sex, selected years 1900-2004. - PowerPoint PPT PresentationTRANSCRIPT
William Kim
Science Research – Period 4
Mrs. Flanagan
Figure 1: Number of people age 65 and over, by age group, selected years 1900-2006 and projected 2010-2050
Need
Figure 2: Life expectancy at ages 65 and 85, by sex, selected years 1900-2004
Need
Knowledge Base
http://www.mayday-info.dk/715.0.html
Knowledge Base
http://www.imsc.res.in/~sitabhra/research/neural/celegans/celegans.gif
http://shirleywho.files.wordpress.com/2008/09/c-elegans_esa.jpg
Fig. 3 Blueberry polyphenols extend lifespan and slow aging in Caenorhabditis elegans.
Literature Review
Wilson et. al. 2006
Literature Review
James Joseph (2007-2009) Tested and still testing fruit polyphenols
and their effects on aging and the brain Tested effects of blackberries,
blueberries, strawberries, plum juice, and other berry fruits
PurposeTherefore the purpose of this experiment is to test the ability of blueberries, blackberries, and other berries in the prolonging of lifespan in C. elegans.
HypothesisNull Hypothesis: The blueberries, etc. will have no effect in prolonging the lifespan of C. elegans.
Alternate Hypothesis: The blueberries, etc. will have a significant effect in prolonging the lifespan of C. elegans.
Polyphenols and Aging Methodology
Caenorhabditis elegansShort lifespan of about 2-3 weeks, rapid generation time, and experiemental flexibilty
Control GroupSample Size = 25Not fed C. elegans medium with blueberry and other polyphenols
Experimental GroupSample Size = 25Fed crude blueberry extract (Vaccinium angustifolium) or a C18 column fraction containing bulk polyphenols in their medium
MeasurementAverage pumping rate of pharynx contraction is recorded at days 8 and 10 of C. elegans life with the use of Motic Plus Camera attached to a stereo microscope.
Statistical AnalysisStatistical analysis by T-test, p<.05, will be used to determine if the collected data is significant.
Protocols for Measurement Commercially prepared single strength wild
blueberry juice (Vaccinium angustifolium) applied to a preconditioned C18 Column
C18 column washed with water to remove fructose, glucose, and organic acids of blueberries, then with 100% methanol to obtain the total polyphenolic fraction
http://www.waters.com/waters/partDetail.htm?locale=101&partNumber=WAT066224
http://www.abnativeplants.com/index.cfm/fuseaction/plants.plantdetail/plant_ID/17/index.htm
Protocols for Measurement
Lifespan scoring was initiated after hermaphrodites completed their final larval molt, on the first day of adulthood
Blueberry extracts were added to NGM agar plates of C. elegans on the first day of the lifespan assay
Pharynx contraction rate recorded with Motic Plus camera attached to a stereomicroscope