plant tissue culture a rice plant growing in nutrient rich agar tissue culture a.k.a...
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Plant Tissue Culture
A rice plant growing in nutrient rich agar
Tissue CultureA.K.A•Micropropagation•In vitro Culture
faperta.ugm.ac.id/newbie/download/pak_tar/Plantbiotechnology/TissuCulturebiotech2007.ppt
Plant Tissue Culture
Learning Objectives Introduction to Tissue CulturePicture Dictation Exercise
Our Learning Objectives in relation to Tissue Culture
Reasons for propagating plants by tissue culture are stated
The culture process is described
Describe some common tissue culture methods Including – meristem culture and embryo culture
Describe how the plant part is selected Describe how the culture medium is made up Explain the timing of plant selection
Introduction to Tissue Culture
Tissue Culture (also known as Micropropagation or In vitro culture) is:
The growing of plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium.
Introduction to Tissue Culture
Plant tissue culture is a bit like the equivalent of ‘Dolly the sheep’ but using plants.
Introduction to Tissue Culture
Cloning Animal Cells Cloning Plant Cells
Both these processes use undifferentiated cells
Picture Dictation Exercise
Take some meristematic cells from a plant.These cells are called an explantPlace the explant on a sterile nutrient rich
agarThe explant grows into a ball of cells This ball of cells is called a callusThe callus then develops roots, stem and
leavesThis is called a plantletTransplant the plantlet into a traditional
growing media Answer
Introduction to Tissue Culture
Is …the technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium
You might say … it is the plant equivalent of dolly the sheep
Back to ContentsBack to picture dictation
What are undifferentiated cells
What are undifferentiated cells In the human body we have lots of different types
of cells
Blood cellsMuscle cellsBones cellsBrain cells
An undifferentiated cell is a cell that does not yet have a specialised job
Next
What are undifferentiated cells
Undifferentiated cells in animals
Stem cells
Undifferentiated cells in plants
?
You tube demonstrations
http://www.youtube.com/watch?v=kje0YczE0Do
The Culture Medium (Media)
The environment surrounding the developing explants must provide the correct ‘necessities’ for plant growth.
Neccessities for plant growth
The Culture Medium (Media)
From this list of neccessaties – which ones are provided by the agar?
Neccessities for plant growth
Hormones in the agar
Two Hormones Affect Plant Differentiation: Auxin: Stimulates Root Development Cytokinin: Stimulates Shoot Development
Generally, the ratio of these two hormones can determine plant development: Auxin ↓Cytokinin = Root Development Cytokinin ↓Auxin = Shoot Development Auxin = Cytokinin = Callus Development
What is Callus development
A callus is a blob of tissue – (mostly undifferentiated cells)
A callus is naturally developed on a plant as a result of a wound
This callus can be left to develop or can be further divided
The culture medium contains a gel (agar) with the proper mixture of nutrients, sugars, vitamins and hormones (growth promotors),
These causes the plant part to grow at very rapid rates to produce new plantlets. It has been estimated that one chrysanthemum apex placed in tissue culture could produce up to 1,000,000 new plantlets in one year.
Tissue Culture Transfer Protocol Dr. Dan Lineberger of Texas A&M University demonstrates the protocol to
transfer African violets from tissue culture containers where they were grown into a small �forest� of cloned plants (called multiplication culture tubes) to tissue containers where the young clones will form new roots.� After the roots are formed, they can be removed and potted into containers. This procedure must be done in the sterile environment of a transfer hood.
Sterilize the surfaces of the transfer hood. Sterilize all tools that touch the plants by first dipping in alcohol them
flaming. African violet clones in a shoot multiplication tube. Remove the cluster of plants in the culture. Insert the cluster of plants into the new culture container. Break up the cluster of plants and spread them out. Seal the container with paraffin film. The culture before transfer (left) and after transfer (right).
Embry Culture
Plant Tissue Culture and G.E