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(

!"

#"

$"!"#$%&'('

0 20 40 60 80 1000

25

50

75

100

Time after LPS (hours)

Sur

viva

l (%

)

TNFR1KOTNFR1WT

*

0 20 40 60 80 1000

25

50

75

100


Sur

viva

l (%

)

DKOIFNAR1KOTNFR1KO

***

*

ns

0 20 40 60 80 1000

25

50

75

100


Sur

viva

l (%

)

IFNAR1KO

IFNAR1WT

**

0 8 16 24 32 40 4824

32

40


Bod

y te

mpe

ratu

re (°

C)

DKOIFNAR1KOTNFR1KO

***** *

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0 2 2 2 6 6 60

500

1000

1500

2000


[IFN!

] (pg

/ml)

********

****

****

Wild typeTNFR1 KOIFNAR1 KO

0 2 2 2 6 6 60

2000

4000

6000

8000

10000


[IFN!

] (pg

/ml) ****

*****

0 2 6 0 2 60

1000

2000

3000

4000

5000


[sTN

FR1]

(pg/

ml)

IFNAR1 WTIFNAR1 KO

*****

****

0 8 8 80

200

400

600

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1000


[TN

F] (p

g/m

l)

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0 6 6 6 60

1

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0 8 8 8 80

200

400

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[IL-F

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pg/m

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Wild typeIFNAR1 KO

TNFR1 KODKO

of 237

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0 8 8 8 80

2000

4000

6000

8000

10000


[MIP

-1!

] (pg

/ml)

****

**** ****

****

*****

****

********

Wild typeTNFR1 KO

IFNAR1 KODKO!"

0 8 8 8 80

500

1000

1500

2000


[IL-1

0] (p

g/m

l)

****

**** ****

****

*****

***

0 8 8 8 80

2000

4000

6000

8000

10000


[MIP

-1!

] (pg

/ml)

****

****

********

****

***

#"

0 8 8 8 80

2000

4000

6000

8000

10000


[KC

] (x

103

pg/m

l)

****

**** ****

****

*

*

0 8 8 8 80

10

20

30

40


[IL-4

] (pg

/ml)

**** ******** ****

**

0 8 8 8 80

20

40

60

80

100


[Ran

tes]

(x 1

03 pg

/ml)

****

********

****

*

0 8 8 8 80

500

1000

1500

2000


[IL-1

3] (p

g/m

l) **

*******

**** ***

0 8 8 8 80

200

400

600

800

1000


[GM

-CS

F] (p

g/m

l) **** **** ********

******

***

0 8 8 8 80

500

1000

1500

2000


[IL-1!

] (pg

/ml)

********

****

****

*****

**

0 8 8 8 80

2000

4000

6000

8000

10000


[IL-1

7A] (

pg/m

l)

********

****

****

***

*

0 8 8 8 80

2000

4000

6000

8000


[IL-1!

] (pg

/ml)

****

****

****

****

0.0592

****

****

**

$"

0 8 8 8 80

20

40

60

80

100

120


[IL-1

2p40

] (x

103

pg/m

l)

****

****

****

****

****

**

0 8 8 8 80

50

100

150

200


[IL-2

] (pg

/ml)

****

********

****

* **0.0554

0 8 8 8 80

200

400

600

Time after LPS (hours)[IL

-9] (

pg/m

l)

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*****

****

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100

200

300

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Lynda Grine(

Date of birth: 22nd of February 1987

Nationality: Belgian

Personal address: Hertstraat 4, 9000 Gent, - Belgium

Tel: +3249/28.00.59

E-mail: [email protected]

Experience

Scientific Researcher - VIB, University of Ghent (Zwijnaarde) January 2011-until present

PhD in Biochemistry and Biotechnology under supervision of Prof. Dr. Claude Libert and Dr.

Roosmarijn E. Vandenbroucke at the Laboratory of Mouse Genetics in Inflammation, Inflammation

Research Center

Scientific coworker - Reliable Cancer Therapies (Strombeek-Beveren) September-December 2010

Freelance scientific coworker, managing database and website content at Reliable Cancer Therapies

Education

PhD in Biochemistry and Biotechnology – University of Ghent January 2011-until present

Doctoral thesis: Profound study of the role of type I Interferons in Tumor Necrosis Factor-mediated

pathologies

Master in Biochemistry and Biotechnology – University of Ghent 2005-2010

Master thesis: Rho GTPase signaling in basal and suprabasal keratinocytes, performed at Biotech

Research & Innovation Centre, University of Copenhagen, Denmark (February-June 2010)

Science and Math (8 hours) – Sint-Lambertusinstituut, Ekeren 1999-2005

Additional courses

Le Français Médical, Ghent 2014

20-hours course by UCT-Ugent

Medical and Scientific Writing, Ghent 2014

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3-day course by Hugin Mugin Research

Drug Discovery Anno 2014, Leuven 2014

2-day course by Flemish Training Network Life Sciences

Basic course Promotion of Health, Brussels 2013

5-day course by the Flemish Institute for Health Promotion and Prevention of Disease

Clinical studies: study design, implementation and reporting, Leuven 2013

2-day course by Doctoral Schools of University of Ghent

Belgian Laboratory Animal Science Course, Ghent 2009

80-hours course for FELASA category C by University of Ghent

First Aid Certificate, Ghent 2009-until present

3-day course by the Red Cross with regular training sessions until present

Fire prevention/fighting course, Ghent 2008

1-day course by the Fire Brigade in cooperation with University of Ghent

Competences and awards

Languages

Dutch: Native proficiency

French: Fluent

English: Fluent (IELTS score: 8.5/9; 2010)

IT skills

Operating systems: MacOSX, Linux (Ubuntu) and Microsoft operating systems

Programming Language: HTML

Software: Microsoft Office and OpenOffice (Word, Excel, Power Point), Adobe Photoshop, Graphpad

Prism, LateX, ImageJ, Mendeley

Life sciences techniques

In vivo work including breeding and genotyping of transgenic mice, handling of animals in various

disease models, protein manipulation (purification, concentration analysis), SDS-PAGE, ELISA,

Western blot, Immunohistochemistrty, Immunofluorescence, Microscopy work, PCR and qPCR

Presentation and communication skills

Posters and talk presentations at national and international meetings

Cytokines, 2012 – Geneva (Switzerland)

Pecha Kucha, 2013 - Zwijnaarde

World Immune Regulation Meeting, 2014 – Davos (Switzerland)

VIB Seminar, 2014 – Blankenberge

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Publications of research articles and reviews

“Dual Inhibition of TNFR1 and IFNAR1 in Imiquimod-Induced Psoriasis Enhances Control of the

Disease.”

Lynda Grine, Lien Dejager, Roosmarijn E. Vandenbroucke, and Claude Libert

Plos One - submitted

“An Inflammatory Triangle in Psoriasis: TNF, Type I IFNs and IL-17.”

Lynda Grine, Roosmarijn E. Vandenbroucke, Lien Dejager, and Claude Libert

Cytokine & growth factor reviews – accepted for publication

“Unintended Ingestion of Topical Imiquimod in Experimental Psoriasis Can Skew Results.”

Lynda Grine, Roosmarijn E. Vandenbroucke, Stefan Lienenklaus, and Claude Libert

In preparation

“TNF-Induced Type I IFNs Control Secretion of IL-17.”

Lynda Grine, Roosmarijn E. Vandenbroucke, Lien Dejager, Riet De Rycke, Marlies Ballegeers, Stefan

Lienenklaus, Mario Köster, Ulrich Kalinke, Leo Joosten, and Claude Libert

In preparation

“Antisense Oligonucleotides against TNFR1 Prevent Toxicity of TNF/IFN! Treatment in Mouse

Tumor Models.”, 2014

Filip Van Hauwermeiren, Roosmarijn E. Vandenbroucke, Lynda Grine, Leen Puimège, Elien Van

Wonterghem, Hong Zhang, and Claude Libert

International journal of cancer

“TNFR1-Induced Lethal Inflammation Is Mediated by Goblet and Paneth Cell Dysfunction.”, 2014

Filip Van Hauwermeiren, Roosmarijn E. Vandenbroucke, Lynda Grine, Sofie Lodens, Elien Van

Wonterghem, Riet De Rycke, Natalie De Geest, Bassem Hassan, and Claude Libert

Mucosal immunology

Reports and data presentation during strategic meetings

Awards

Innovation by Science & Technology Grant for Doctoral project (Brussels, December 2010)

Pecha Kucha presentation award by Inflammation Research Center (Zwijnaarde, 2013)

Lynda GRINE – +32499/28.00.59 – [email protected]

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