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Page 1: Percoll Reference list · Ion Exchange Chromatography Principles and Methods 18-1114-21 Affinity Chromatography ... Gel Filtration Principles and Methods 18-1022-18 Handbooks from

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18-1148-50

Edition AA

PercollReference list

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Antibody PurificationHandbook18-1037-46

The Recombinant Protein HandbookProtein Amplification and Simple Purification18-1142-75

Protein PurificationHandbook18-1132-29

Ion Exchange ChromatographyPrinciples and Methods18-1114-21

Affinity ChromatographyPrinciples and Methods18-1022-29

Hydrophobic Interaction ChromatographyPrinciples and Methods18-1020-90

Gel FiltrationPrinciples and Methods18-1022-18

Handbooksfrom Amersham Biosciences

Reversed Phase ChromatographyPrinciples and Methods18-1134-16

Expanded Bed AdsorptionPrinciples and Methods18-1124-26

Chromatofocusingwith Polybuffer and PBE50-01-022PB

Microcarrier cell culturePrinciples and Methods18-1140-62

PercollMethodology and Applications

18-1115-69

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PercollReference list

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Introduction ................................................................................................. 5

Subject Index ............................................................................................... 7

List of references ........................................................................................ 20

Author index .............................................................................................. 92

Ordering Information ................................................................................. 116Products for Cell Separation and Culture ....................................................................................... 116Products for Purification of RNA .................................................................................................. 117Products for Purification of DNA .................................................................................................. 117Kits for cDNA Synthesis .............................................................................................................. 117

Content

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Introduction

Percoll™ is a density gradient medium designed to separate cells, subcellular particles and virusesunder gentle, physiological conditions. It is supplied as a sterile colloidal suspension comprised ofsilica particles, 15–30 nm in diameter, coated with polyvinylpyrrolidone (PVP). Because it consistsof heterogeneously sized particles, Percoll will spontaneously form a density gradient when centrifuged.This novel medium possesses the following characteristics, which make it useful for a variety ofapplications:

• covers a sufficient density range for isopycnic banding of all biological particles of interest

• possesses physiological ionic strength and pH

• is iso-osmotic throughout the gradient

• has low viscosity

• is non-toxic

• is supplied sterile and is resterilizable

• will form self-generated gradients by centrifugation at moderate g-forces

• is compatible with biological materials and does not penetrate membranes

• is easily removed from purified materials

• does not affect assay procedures

• will not quench radioactivity assays

• can form both continuous and discontinuous gradients

• forms stable that can be pre-made to give reproducible results

• simplifies gradients analysis when used with the Density Marker Beads

• does not affect processing of isolated materials

Since the development of Percoll by Amersham Biosciences and its subsequent launch in 1977,thousands of scientific publications from research laboratories throughout the world have shownthis product to be the centrifugation medium of choice for density-based separation of cells, subcellularparticles, and viruses. This manual was developed to provide researchers with an extensive list ofreferences that have cited the use of Percoll for a variety of applications. It contains 1163 citationsthat are cross-referenced by author and subject (e.g. cell or organelle type, tissue source, and species).A quick glance at the subject index shows the wide range of applications for which Percoll has been used.

Detailed technical information on Percoll, including information on the preparation and use ofPercoll gradients, can be found in the manual Percoll: Methodology and Applications, which isavailable upon request.

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Subject Index

AADCC effector cells 562Adipocytes 591Adipocyte plasma membrane 831Adreal zona glomerulosa cells, rat 637Adrenal gland, bovine 78Affinity density perturbation 278African swine fever virus 570Algae, blue green 56Algae, marine 28, 201Alveolar cell populations, guinea pig 797Alveolar macrophage subpopulations,bovine 867Alveolar macrophage rat 780, 834Alveolar macrophage subpopulations,human 811Alveolar pneumocytes, rat 603Amastigotes 580Aminotic cells 334, 362Amoebapore 561Antigen (carcionembryonic antigen) 1136Antigen presenting B cells 572Apical plasma 683Apical plasma membrane 606, 683Apoptotic cells, human 1071Ascites sarcoma cells 557Astroglia 598Autolysosomes 864Autophagosomes 864Azurophilic granules 727Azygospores, fungi 246

BB cell 416, 420B cell fractionation, human 750B cell fractionation, murine 749B cell lymphoblasts, mouse spleen 141, 144, 345B cell, resting 749B cell, resting and non-resting 704, 732B lymphoblasts isolated on Ficoll 769B lymphocyte depletion 563B lymphocyte subpopulations 718, 783, 796B lymphocytes 538B lymphocytes, human 229, 331, 376, 487,

494, 501, 506, 509,512, 513

B lymphocytes, murine spleen 142, 796Babesia bigemina, protozoa 1080Babesia bovis, protozoa 1083Babesia equi, protozoa 1081

Bacillus subtilis 129Bacteria 632, 647, 881Bacteria, Bacteroides 1074Bacteria, Ehrlichia risticii 1075, 1076Bacteria, Porhyromonas gingivalis 1077Bacteria, Treponema pallidum 1078Bacteria, Theileria 1079Bacteria, subpopulations 788, 810Bacterial attachment assay 767Bacterial attachment to epithelial cells 767Bacterial cells walls 235, 785, 788Bacterial minicells 663Bacteriorhodopsin sheets 193Bacteroids, root nodule 205, 849Barley leaf and seed plastids 1126Barley seed protoplasts 1124Basal cell separation 694Basophil purification 719Basophils, guinea pig 548Basophils, human 347, 438, 967, 968,

969, 970, 971, 972, 973Basophils, rat 974Basophil subpopulations, human 801Bean leaf mitochondria 1121BFU-e 675Blood cells, enzyme activity and Percoll 424Blood cells, human, chronicgranulomatous disease 94Blue-green bacteria, marine micro-algae 60Bone marrow cells 595, 626, 661, 664,

665, 666, 675, 700,737

Bone marrow cells, canine 676Bone marrow cells, examination 672Bone marrow cells, feline 1047Bone marrow cells, guinea pig 157, 187Bone marrow cells, human 98, 155, 187, 217,

272, 520, 815, 1027,1028, 1029, 1030, 1031,

1032, 1033, 1034Bone marrow, human (HIV-infected) 1029Bone marrow cells,human leukemic 814, 884, 1032Bone marrow cells, marmoset 1035Bone marrow cells, monkey 1037Bone marrow cells, murine 187, 236, 712, 798,

820, 868, 1038, 1039,1040, 1041, 1042, 1043,

1044, 1045Bone marrow cells, primate 1036Bone marrow cells, rabbit 38, 187

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Bone marrow cells, rat 52, 157, 187, 543,1046

Bone marrow transplantation 661Bovine, granule 78Bovine adrenal gland 78Bovine lymphocytes 915Bovine peripheral blood leukocytes 783Bovine plasma membrane 78, 1096Bovine retinal pigment epithelium 695Bovine sperm fractionation 778Bovine spermatozoa 1024Brain capillary cells, rat 37, 170Brain lysosomes 577, 871Brain synaptosomes 786Brain tissue 599Brain, edema 517Brush border membrane vesicles 770Brush border membranes 746Brush border membranes, rabbit 234Brush border membranes, rabbit enterocyte 125Brush border membranes, rat adrenal cortex 143Brush cells, rat stomach 1151Buffalo lysosomes 1106Buoyant density 635Buoyant density determination 642

CCaedibacter 751Calvaria cell subpopulations 824Canine lymphocytes 908, 909Capillary cells, rat brain 37, 170Carcinoembryonic antigen 1136Cardiocytes, rat 198, 281Carrot suspension cell nuclei 1125Cell cytotoxicity, effector cell 399Cell cytotoxicity, mechanism 359, 375, 385,

393, 491Cell cytotoxicity, suppression 432Cell depleted 563Cell differentiation 700Cell line, Subpopulations of MCF-7 782Cell maturation 700Cell separation 697, 1069Cell separation in Percoll (review) 289, 1069Cell separation techniques 461Cell size and state of differentiation 626Cell volume and buoyant density 645, 647Cells, anti-Ia-reactive, urinary tract 373Cells, bronchoalveolar, guinea pig 473, 484Cells, granulosa, porcine 377, 418Cells, Hodgkin´s disease 419Cells, K562 uptake of Fe 387Cells, Leu 7+ (HNK-1+) 502

Cells, malignant ascites 411Cells, pleural effusions 431Cells, populations of rat liver 444Cells, synovial lining 471Cellular uptake of Percoll 288, 303Cerebellar neurons 598Cerebral cortex, hamster 332Cerebral cortex, mouse 332CFU-c 666, 675Charot-Leyden crystals 304Chicken muscle nuclei 832Chief cells 569, 809Chlamydia trachomatis 434, 503Chloroplast 12, 49, 58, 76,

88, 109, 135, 152,209, 322, 437, 478,

714Chloroplast, maize 564Chloroplast, pea leaf 76Chloroplast, spinach leaf 76, 88Chloroplast, tobacco leaf 1122Chloroplast DNA without CsClgradient purification 771Chloroplast isolation 768Chloroplast purification 735CHO cells plasma membrane 1098Chondrocytes 629, 821Chorion laeve cells 630Chromaffin granules, bovineadrenal 78, 89, 121, 133,

186, 216, 220, 232Chromaffin secretory vesicles 722Chromosome purification 677Chromosomes 280Clara cells, guinea pig 1146Clustering, dendritic cells and T cells 608Clostridium cell walls 785Coated vesicles, bovine brain 326Cochlear hair cells 800Colony forming cells, human 184, 217Colony forming cells, murine 236Comparison - continuous vs.discontinuous gradients foreosinophil isolation 829Comparison - Percoll and Ficoll-Paque 547Comparison - swimup vs. gradientseparation of spermatozoa 857, 876Comparison - sucrose, metrizamideand Percoll 523, 884,Contamination removal fromcell culture 845, 874Cord blood cells, human 1150Corneal dystrophy 699Corpus callosum, rat 413Corpus luteum cells, rat 146, 199

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Cowdria ruminantium 848Crayfish hemocytes 560Cryptosporidium oocysts 696, 705, 877Cryptosporidium parvum merozoites 1157Cryptosporidium sporozoites 877CTL and NK granules 609CTL granules 687Cultured rat hepatocytes 741Cyanobacteria 566Cystol 1134Cytolytic granules 668Cytoplasmic bodies 607Cytoplasmic granules 687Cytoplasts 721Cytoplasts, plants 1123Cytotoxic lymphocytes, human 218Cytotoxic lymphocytes, chicken 851Cytotoxic T lymphocytes granules 668

DDead cell removal 221, 241, 262, 283,

1070Decidual cells, human 805Dendritic cells, human thymus 826Dextran sulphate as B cell mitogen 769Diatoms, marine micro-algae 60Dictyostelium disconideum cells 214, 439Dictyostelium discoideum prespores 781Dictyostelium discoiedum pre-stalk cells 781Dictyostelium discoideum spores 194, 339Dinoflagellates 582Dinoflagellates, marine micro-algae 60Discontinuous osmolality gradients 527DNA. plant high MW 738Dorsal root ganglia neurons 784, 856Duct cells, porcine 1145Dynamic density gradients 521

EEAE effector cells, rat 276Effect of ionic strength 775Effector cells 539Embryos, scallop (Pecten maximus) 669Endoplasmic reticulum 727Endoplasmic reticulum, human liver 13, 200Endosomes, human 1130, 1131Endosymbiotic bacteria 751Endothelial cells 408, 723Endothelial cells, bovine retinalmicrovascular 309Endothelial cells, human 1067Endothelial cells, porcine 803Endothelial cells, rat 650

Endothelial cells, rat brain 37, 170Endothelial cells, rat liver 880Enrichment 777Entamoeba histolytica digestive vesicles 561Entamoeba histolytica, cysts and precysts 467Entamoeba histolytica, protozoa 1084Entamoeba invadens, cysts and precysts 467Entercytes, murine 428Eosinophilic leukocytes 600Eosinophils 594, 600, 747Eosinophils, bovine blood 148, 253Eosinophils, guinea pigperitoneal exudate 139Eosinophils, human 97, 297, 475, 573,

812, 829, 958, 962,963, 964, 965, 966

Eosinophils, human, subpopulations 812Eosinophils, murine 100, 119, 344Eosinophils, purification usingdifferent gradient media 747Epidermal cell fractionation 694, 729Epidermal cell subpopulations 715Epidermal cells, guinea pig 273Epidermal cells, rat 172Epithelial cells with adhered bacteria 731Epithelial cells, guinea pig intestine 10Epithelial cells, human stool 1158Epithelial cells, immature rat prostate 325Epithelial cells, murine intestine 7Epithelial cells, murine mammary 222Epithelial cells, murine skin 312Epithelial cells, rat liver 32Epithelial cells, rat pulmonary Type II 795Epithelium, murine vagina 652Equine leucocytes, single step purification 617Equine neutrophils 706Equine monocytes 858, 930Erythrocyte ghost isolation 242, 685, 692Erythrocyte-binding cells, mice 427Erythrocytes 701, 725Erythrocytes, age separation 584, 614, 760, 761,

763, 764, 798Erythrocytes, bullfrog 128, 349Erythrocytes, falciparum-infected 601Erythrocytes, human 55, 66, 77, 94,

108, 114, 140, 164,350, 407, 498, 760,931, 932, 933, 934,935, 936, 937, 938,

939, 940Erythrocytes, human, parasite-infected 319Erythrocytes, infected 623Erythrocytes, Mastomys natalensis 941Erythrocytes, monkey, parasite-infected 263

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Erythrocytes, murine 163, 264, 798, 942,943, 944

Erythrocytes, murine,parasite-infected 293, 320, 410Erythrocytes, parasitized 685, 854Erythrocytes, Plasmodium vivaxinfected 854Erythrocytes, rabbit 946Erythrocytes, Rana catesbeiana 128, 349Erythrocytes, rat 151, 285, 945Erythrocytes, sheep 614Erythrocytes, stressed 762Erythrocytes, trout 947Escherichia coli 129, 260, 368, 405,

413, 493, 1137Escherichia coli, inner andouter membranes 1137

FFaba leaf mitochondria 1121Feline bone marrow 1047Fibroblast depletion 723Fibroblast-like cells, non-myogenic 680Fish lymphocytes 624Flagella, human sperm 1128, 1129Food, microbial numbers 402Fractionation of irradiated thymocytes 659Frog melanotrope cells 1142Frog pituitary cells 1142Fungal spores 182, 246Fungi azygospores 246Fungi microbodies 1135Fungi (Neurospora sp.) nuclei 1127Fungi (Penicillium sp.) plasmamembrane 1100, 1101

GGametocytes, Plasmodiumfalciparum 319Gametocytes, Plasmodiumyoelii nigeriensis 319Gastric heavy microsomal membranes 634Gastric mucosa cells, pig 569Germ cells, murine 367Germinal centre B cells 736Germinal centre cells, rabbit appendix 250Glial progenitor cells, rat brain 1148Glomeruli, rabbit kidney 206Glycosomes 202Glyoxysomes 294, 296Golgi 597Golgi complex 727Golgi elements 1134Golgi elements, rat liver 318, 406

Gonadotrope cells, porcine pituitary 1143Gradient formation technique 571Granule 609, 687, 822Granule, bovine 78Granule, human 1112, 1113, 1114,

1115, 1116Granule, murine 17Granule, rat 1117Granule, sea urchin 1118Granulocyte membrane vesicles,guinea pig 305Granulocytes, bovine 253Granulocytes, human 55, 94, 190, 215,

238, 316, 369, 511Granulocytes, human colostral 210Granulocytes, improved separation 611Granulosa.lutea cells, human 1160Granulosa-luteal cells, rat 1161Green leaf plant mitochondira 12Growth hormone producing cells, rat 882Guinea pig clara cells 1146

HHaemocytes, lysis, crayfish 445Haemocytes, marine decapoids 446Haemopoietic progenitor cells 667, 671, 712Hamster 698Hamster spermatozoa 1025Hatching gland cells, teleost 168HeLa cells 23Hemocytes, crayfish 560Hemocytes, moth (Pheudoplusiaincludens) 1141Hepatitis B surface antigen, human 26Hepatocyte cytosheres 607Hepatocytes, human 808Hepatocyte membrane, rat 741Hepatocyte, asialoglycoproteinreceptor 354Hepatocytes from a single rat liver 650Hepatocytes, rat 33, 64, 71, 159,

270, 650Herpes simplex virus 56, 156Herpes virus, equine 21Heterokaryocytes 759Hexose-transporting cells 625Hippocampal synaptosomes, rat 870Human apoptotic cells 1071Human bone marrow cells 1027, 1028, 1029,

1030, 1031, 1032,1033, 1034

Human bone marrow cells,(HIV)-infected) 1029Human bone marrow cells (immune) 1029

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Human bone marrow cells (leukemic) 1032Human cord blood cells 1150Human endoplasmic reticulum 13Human endosomes 1130, 1131Human endothelial cells 1067Human eosinophils 962, 963, 964, 965,

966, 967, 968, 969,970, 971, 972, 973

Human erythrocytes 55, 66, 77, 94,108, 114, 140, 164,350, 407, 498, 760,931, 932, 933, 934,935, 936, 937, 938,

939, 940Human Leydig cells 986, 987, 988, 989Human liver cells 975Human lymphoblasts 1072Human lymphocytes 11, 16, 40, 69,

83, 891, 892, 893,894, 896, 897, 898,899, 900, 901, 902,903, 904, 905, 906,

907Human lysosomes 13, 1102, 1103, 1132Human macrophages 928, 1048, 1049, 1050,

1051, 1052, 1053Human megakarocytes 115Human melanosomes 1132Human mitochondria 13Human monocytes 27, 34, 40, 48,

57, 69, 94, 95,101, 124, 137, 149,

(153), 174, 180, 183,203, 212, 226, 230,268, 352, 366, 369,435, 836, 903, 916,917, 918, 919, 920,921, 922, 923, 924,925, 926, 927, 928,

929Human natural killer cells 61, 948, 949, 950,

951Human neutrophils 955, 956, 957, 958,

959, 960, 961Human ovarian cells(granulosa-lutea cells) 1160Human pancreatic islets 5Human placenta mitochondria 713Human plasma membranes 13, 54, 1089Human pneumocytes 1140Human sperm flagella 1128, 1129Human spermatozoa 1001, 1002, 1003, 1004,

1005, 1006, 1007, 1008,1009, 1010, 1011, 1012,1013, 1014, 1015, 1016,1017, 1018, 1019, 1020,

1021, 1022, 1023Human stool epithelial cells 1158

Human sub-cellular fraction 133, 727, 1069Hyaline cells 686Hyalinocytes 686Hybrid gradients 710Hybrid hybridoma enrichment 748Hybrid hybridomas 777Hybrid protoplasts 759Hybridomas 748Hydrolytic granules, human neutrophils 822Hypersensitivity, delayed-type, mice 449

IInfected bovine erythrocytes 730Infected erythrocytes 685, 892Influence of temperature on recoveryof cytospheres 607Inner and outer membranes, E. coli 1137Insect haemocyte types 622Interstitial cells 764Interstitial cells, murine testes 568, 658Interstitial cells, pig 682Intestinal cells, guinea pig epithelial 10Intestinal cells, murine epithelial 7Intra epithelial lymphocytes, murine 589Intracellular parasites 122Intracellular symbionts, elder aphid 374Intracytoplasmic inclusions 645Islets, human 726Isolation hybridoma 777Isolation of individual andclustered chromosomes 677Isopora gondii cysyts 269

KKeratinocyte 694Keratinocytes, guinea pig 273Keratinocytes, murine 312Keratocytes 699Kidney cells (duct cells), porcine 1145Kidney components, rabbit 206Killer lymphocytes 562Küpffer cells 650Küpffer cells, murine 154Küpffer cels, rat 20, 880Kurloff cells 533

LLactotrops, rat adenohypophysis 19, 382LAK cell subpopulations 716LAK precursors 665Langerhan´s cells 729Large granular lymphocytes 530, 545, 636

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Leaf mitochondria 528Lectin, surfactant protein D 1153Leishmania amastigotes 122, 489Lemna gibba nuclei 549Leucocytes 534, 554, 635, 719Leucocytes, basophilic, human 417Leucocytes, bovine 783Leucocytes, canine blood 360Leucocytes, equine 617Leucocytes, fich 850Leukaemic blast cells, human 262Leukemic cells, human 901Leukocytes, leukaemic 681Leydig cells 568Leydig cells, fish 838Leydig cells, human 986, 987, 989, 989Leydig cells, murine 31, 105, 310, 990Leydig cells, porcine 682, 991Leydig cells rat 31, 92, 171, 282,

310, 313, 353, 649,656, 743, 804, 862,878, 889, 992, 993,994, 995, 996, 997,

998, 999, 1000Leydig tumor cells, rat 176LGL 530, 545, 636Lipid bodies 477Lipid vesicles, Torpedo californica 392Liposome marker in electron microscopy 644Liposomes 392Liver biopsy organelles 13Liver cells, human 975Liver cells, rat 20, 24, 33, 55,

71, 976, 977, 978,979, 980, 981, 982,

983, 984, 985Luminal vesicles, rat kidney 746Lung cells 587Lung wall cells, rat 734Luteal cells, rat 18, 146Lymph node cells, murine 8, 51Lymphoblasts 537Lymphoblasts, human 16, 277, 302, 1072Lymphoblasts, human T cell 83, 93, 283, 337Lymphoblasts, murine 36, 265, 508Lymphoblasts, murine spleen B cell 141, 144Lymphoblasts, murine spleen T cell 115, 224Lymphocyte populations 567, 725Lymphocyte stimulatory capacity 567Lymphocytes, bovine 915Lymphocytes, catfish 850Lymphocytes, chicken 851Lymphocytes, canine 908, 909Lymphocytes, E rosette-negative 567Lymphocytes, fish 624

Lymphocytes, guinea pig 443Lymphocytes, human 11, 15, 16, 40,

55, 63, 69, 83,94, 101, 226, 229,

254, 316, 336, 363,385, 389, 428, 793,891, 892, 893, 894,896, 897, 898, 899,900, 901, 902, 903,904, 905, 906, 907

Lymphocytes, human NK 61, 67, 96, 162,169, 243, 245, 300,

323, 329, 33 341,346, 351, 404, 472

Lymphocytes, human, colostral 207, 210Lymphocytes, human, cord blood 11Lymphocytes, human, interferon 358Lymphocytes, human, lymphoma 116Lymphocytes, human, mitogen-stimulated 11Lymphocytes, human, sub-fractionation 41, 261, 476, 499Lymphocytes, human, suppressor 44, 432Lymphocytes, human, tonsil 793, 892, 893, 896,

900, 905Lymphocytes, human, T 73, 131, 175, 331,

338, 340, 356, 357,372, 395, 457, 458,

463, 487, 514Lymphocytes, mixed culture 348Lymphocytes, monkey 830Lymphocytes, monkey, NK 789Lymphocytes, murine 284, 482, 910, 911,

912, 913, 914Lymphocytes, murine, intestinalmucosa 257, 271Lymphocytes, murine, NK 196, 885Lymphocytes, murine, splenic 355, 911, 912Lymphocytes, murine, subpopulations 796Lymphocytes, murine, tumour-associated 150Lymphocytes, rat peripheral blood 317Lymphocytes, rat, NK 252, 266, 307, 328,

333Lymphocytes, sheep 251Lymphocytes, trout 559Lymphoid cells, intestinal, mice 433Lymphokine-activated killer cells 665, 716Lysosomal membranes 713Lysosomal membranes, rat liver 709Lysosomes 597, 618, 713, 772,

1134Lysosomes, brain 577Lysosomes, buffalo 1106Lysosomes, chicken epiphysis 21Lysosomes, hamster fibroblasts 188Lysosomes, human 13, 1102, 1103, 1132

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Lysosomes, human fibroblasts 365, 787Lysosomes, human liver 13, 87Lysosomes, human lymphoblast 138Lysosomes, murine brain 792, 871Lysosomes, murine liver 1104Lysosomes, murine spleen 21Lysosomes, porcine 1107Lysosomes, porcine thyroid 827Lysosomes, rat 24, 132, 1105, 1106Lysosomes, rat fibroblasts 21Lysosomes, rat heart 123Lysosomes, rat liver 21, 24, 27, 145

MMacaque spermatozoa 1026Macrophage adhesion, effect of Percoll 303Macrophage 536, 639Macrophages, bovine alveolar 867Macrophages, human 928, 1048, 1049, 1050,

1051, 1052, 1053Macrophages, human alveolar 811, 1048, 1049,

1050, 1051, 1052,1053

Macrophages, human ascitic 34Macrophages, human peritoneal 495Macrophages, Listeria monocytogenes 464Macrophages, murine 30, 391, 460, 505,

1054, 1055Macrophages, murineperitoneal 30, 130, 208, 274,

479Macrophages, rat 1055, 1056, 1057, 1058Macrophages, rat alveolar 780, 802, 834, 1055,

1056, 1057, 1058Macrophages, suppressor 454Maize leaf and seed plastids 1126Maize leaf mitochondria 1121Malaria 601Malarial blood fractionation 740Malarial erythrocytes 601Malignant cells 672Malignant effusions 574Mammalian cell fractionation 844Mammotrope, rat 886Marine sponge spherulous cells 835Marine micro-algae dinoflagellates 60Marine micro-algae diatoms 60Marine micro-algae blue-green bacteria 60Marmoset bone marrow cells 1035Mast cell granules, mouse mastocytoma 17Mast cell granules, rat 92Mast cells 536Mast cells, human lung 586Mast cells, murine 8, 177, 1059

Mast cells, rat peritoneal 9, 104, 640, 1060,1061, 1062

Mastomys natalensis, erythrocytes 941Megakaryocytes, canine 676Megakaryocytes, guinea pig 157Megakaryocytes, human 155, 612, 626Megakayocytes, murine 820, 868Megakaryocytes, rat 157Meiofauna 295Melanocytes, guinea pig 167Melanocytes, human 380Melanosomes, human 1132Melanotrope cells, frog pituitary 1142Membrane fraction 653, 683, 741Membrane isolation 606, 709Membrane organelles 758Membrane vesicles, Electrophorus 189Membrane vesicles, rat kidney 237, 255, 746Membrane, acetylcholine receptor-rich 558Membrane, fish electric organ 558Membrane, peroxisomal 426Membranes 683, 713Membranes, brush-border, intestinal 396, 469Membranes, separation ofinner and outer 1137Membranes, yeast 758Merozoites, Cryptosporidium parvum 1157Mesophyll protoplasts 759Micro-algal cell density 620Microalgae, marine 60, 295Microbial cells 642Microbodies, fungi 1135Microfilariae 179Microgravimetry 599Micronuclei, paramecium 1154Microorganisms 642, 645Microorganisms, buoyant density 647Microorganisms, density changes 646Microsomal membranes, heavy and light 634Microsomal vesicles, murine 275Microsomes, BHK 21 cells 188Microsomes, chicken 459Microsomes, glioma cell line 806Microsomes, rat hepatocyteMicrospores, plant 165Microsporidian spores 239Microvessels, rat cerebral 1139Microvessels, rat epididymal 259Microvilli, rat tumour 120, 191Minicells 663Mitochondria 708, 710, 758Mitochondria, avocado 383, 384Mitochondria, bean leaf 1121

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Mitochondria, bovine skeletal muscle 103, 147Mitochondria, brain 577Mitochondria, carrot root 739Mitochondria, castor bean seed 1119Mitochondria, faba bean leaf 1121Mitochondria, fibroblast 728Mitochondria, fungus 296Mitochondria, green leaf plant 12Mitochondria, hamster fibroblast 188Mitochondria, human 13Mitochondria, human liver 13Mitochondria, human placenta 713Mitochondria, maize leaf 1121Mitochondria, Manihot esculenta(cassava) tuber 852Mitochondria, plant 12, 43, 106, 185,

294, 324, 755, 1119,1120, 1121

Mitochondria, porcine heart 1108Mitochondria, potato tuber 415, 651Mitochondria, protozoa 1110Mitochondria, rabbit heart 1108Mitochondria, rabbit reticulocyte 784Mitochondria, rat brain 872Mitochondria, rat liver 86, 200, 327, 515,

1109Mitochondria, soybean leaves 724Mitochondria, sugar beet leaf 1121Mitochondria, sunflower seed 1120Mitochondria, spinach leaf 613Mitochondria, tobacco leaf 1121Mitochondria, turkey sperm 1111Mitochondria, wheat leaf 1121MLC matching 681Mold mycelia 845Monkey aortic lysosomes 703Monkey bone marrow cells 1037Monkey lymphocytes 830Monkey NK cells 789Monkey placenta syncytiotrophoblasts 1156Monocytes 725Monocytes, equine 858, 930Monocytes, human 27, 34, 40, 48,

57, 69, 94, 95,101, 124, 137, 149,153, 174, 180, 183,203, 212, 226, 230,268, 352, 366, 369,435, 836, 903, 916,

917, 918, 919, 920,921, 922, 923, 924,925, 926, 927, 928,

929Monocytes, human, colostral 210Monocytes, subfractionation 588

Mononucleated myogenic cells 680Moth (Pseudoplusia includens)hemocytes 1141Motile, virulent Treponema pallidum 593Mouse (see Murine)Mung bean hypocotyl mitochondria 755Murine bone marrow cells 1038, 1039, 1040,

1041, 1042, 1043,1044, 1045

Murine erythrocytes 942, 943, 944Murine fibroblast 702Murine granule 17Murine leukemia virus 693Murine Leydig cells 990Murine lymphocytes 910, 912, 913, 194Murine lysosomes 1104Murine macrophages 30, 1054, 1055Murine mast cells 8, 1059Murine natural killer cells 952, 953, 954Murine neutrophils 70Murine pancreatic islets 5Murine thymocytes 62, 1063Muscle cells 680Muscle cells, murine 742Mycelia 845Mycobacterium leprae 615Mycroplasma-like organism (MLO) 1088Myeloblasts 717Myelocytes 717Myocardial cells, rat 249Myocytes, rabbit uterus 1152Myogenic cells, chicken embryos 680Myxosoma cerebralis spores 828

NNasopharyngeal secretions 643Natural killer lymphocytes 636Natural killer lymphocytes,human 61, 67, 96, 162,

169, 243, 245, 300,329, 333, 341, 346,351, 363, 372, 388,397, 398, 412, 441,450, 465, 483, 490,492, 496, 497, 516,948, 949, 950, 951

Natural killer lymphocytes,monkey 789Natural killer lymphocytes,murine 196, 430, 541, 636,

779, 860, 952, 953,954

Natural killer lymphocytes,in muscular dystrophy 885

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Natural killer lymphocytes,rat 252, 266, 307, 328,

333, 468Nephron components, rabbit 206Neuroblastoma plasma membrane 596, 610Neurons, rabbit 370, 480, 856Neurons, murine 856Neurospora nuclei 394Neutrophil enrichment 575Neutrophil plasma membrane 633, 638Neutrophils 573, 578, 594, 706,

717Neutrophils, bovine blood 148, 253Neutrophils, human 311, 807, 873, 955,

956, 957, 958, 959,960, 961

Neutrophils, hydrolytic granules 822Neutrophils, murine 70Neutrophils, porcine 638Neutrophils, rabbit peritoneal exudate 136Non-ciliated bronchiolar epithelial(clara) cells, guinea pig 1146Non-synaptic mitochondria 657Nuclei 597, 1127Nuclei, carrot suspension cells 1125Nuclei, chicken muscle 832Nuclei, fungi (Neurospora sp.) 1127Nuclei- physarum 842Nuclei, plant 841, 842, 843Nuclei, slime mold 621Nuclei, striated muscle 832Nuclei, wheat embryo 197Nucleoplasts 721

OOligodendroglia, calf 204, 244Oocysts purification 696, 705, 877Organelle separation 775Organelles, plant 771Oryzias latipes hatching gland cells 168Osmocentrifugation 571Osmolality adjustment 555Osmolality and separation 711Osmolality adjustment foroptimized separation 635Osteoclasts 585Osteoclasts 585Ovarian cells (granulosa-lutea cells),human 1160Ovarian cells (granulosa-lutea cells),rat 1161Ovarian cells (thecal-intestitial),rat 1159Oyster haemocytes 696

PPancreatic acinar carcinoma,rat subpopulations 308Pancreatic islet cells, rat 290Pancreatic islets, human 5Pancreatic islets, mouse 5, 75, 102, 118Pancreatic islets, rat 118, 306, 711Paramecium, micronucleus 1154Paramecium, surface layer (cortex) 1147Paramecium endosymbionts 751Parapox virus 91Parasites 691, 730Parasites, intracellular 122Parasites, malarial 740Parathyroid cells, human 107Parathyroid glands, human 1, 2, 3, 35Parenchymal cells, rat liver 55, 485Parietal cells 569, 576Parietal cells, rat 178Pea leaf and seed plastids 1126Pea leaf chloroplast 76Pepsinogen granules 840Perch retina cells 1138Percoll as electron dense marker 644Percoll gradients containing PEG,Ficoll and BSA 714Percoll metrizamide 710Percoll removal 570Percoll removal by gel-filtration 275Percoll, radioactive labelling 554Pericytes, rat brain 37Peritoneal cells 575Peritoneal cells, murine 130, 274Peritoneal exudate cells, murine 8, 303Peritoneal exudate cells, rat 9Peritoneal macrophages 639Peroxisomes 474, 758, 1132Peroxisomes, pea leaf 654Peroxisomes, rat liver 53, 112, 200, 223,

343, 455Peroxisomes, spinach leaf 378, 613Physarum polycephalum nuclei 621Phytoplankters, freshwater 201Phytoplankton, marine 28Pituitary cells, frog 1142Pituitary cells, porcine 1143Pituitary cells, rat 707Placenta cells, syncytiotrophoblasts 1156Plant chloroplasts 766Plant DNA 738Plant mitochondria 43, 1120, 1121Plant organelles mitochondria 766Plant secretory cells 865

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Plasma membrane 590, 591, 610, 633,657, 695, 776, 806

Plasma membrane from Acanthamoeba 823Plasma membrane from human platelets 627Plasma membrane vesicles 532Plasma membrane, bovine 78, 1096Plasma membrane, bovine adrenalchromaffin cell 186Plasma membrane, CHO cells 1098Plasma membrane, Entamoebahistolytica 561Plasma membrane, fungi(Penicillium sp.) 1100, 1101Plasma membrane, glioma cell line 806Plasma membrane, human 13, 54, 1089Plasma membrane, human liver 13Plasma membrane, Krebs ascites cell 291Plasma membrane, neutrophil 638Plasma membrane, oligodendrocytecell line 837Plasma membrane, rat 33, 1089, 1090, 1091,

1092, 1093, 1094, 1095Plasma membrane, rat adipocyte 110, 831Plasma membrane, rat brain 1091Plasma membrane, rat epithelial cell 111Plasma membrane, rat kidney cortex 237, 255Plasma membrane,rat liver 33, 604, 1089, 1093,

1094, 1095Plasma membrane, rat uterus 1090Plasma membrane, retinalpigment epithelium 583Plasma membrane, sheep adipocytes 1097Plasma membrane, skate 1099Plasma membrane, spheroplasts 565Plasmodium, protozoa 1082Plasmodium chabaudi-infectederythrocytes 692Plasmodium falciparum-infected erythrocytes 551Plasmodium falciparum, antibodies 519Plasmodium falciparum, gametocytes 319Plasmodium falciparum, merozoites 447, 451Plasmodium falciparum, schizonts 440, 442, 451,

452Plasmodium infected erythrocytes 623, 740Plasmodium knowlesi, clones 486Plastids, barley leaf and seed 1126Plastids, maize leaf and seed 1126Plastids, pea leaf and seed 1126Platelet granules, pig 267Platelet membranes, human 54, 298Platelets 684Platelets, human 287, 790, 791Platelets, human, subpopulations 790, 791

Pneumocystis carinii trophozoites 1155Pneumocytes, human 1140Pollen, mitotic 869Pollen, premitotic 117Polymorphs 534Porcine brain endothelial cells 803Porcine duct cells 1145Porcine gonadotrope cells 1143Porcine kidney cells 625Porcine kidney cells (duct cells) 1145Porcine Leydig cells 991Porcine lysosomes 1107Porcine mitochondria 1108Porcine pituitary cells 1143Potato tuber mitochondria 651Preadipocytes, rat 6Pre-spore, Dictyostelium discoideum 214, 781Pre-stalk cells, Dictyostelium discoideum 781Primate bone marrow cells 1036Progentior cells, rat brain 1148Prolactin cells, rat 707, 866, 882Promyelocytes 717Proplatelets 678Protein bodies, barley endosperm 227Protein D 1153Protein determination in the presenceof Percoll 240, 518, 813Protoplast evacuolate 733Protoplast vesicles, pea 301Protoplast, hybrids 759Protoplasts 209, 409Protoplasts, barley seed 1124Protoplasts from chlamodysporesand mycelium 524Protozoa, Babesia bigemina 1080Protozoa, Babesia bovis 1083Protozoa, Babesia equi 1081Protozoa, Entamoeba histolytica 1084Protozoa, Plasmodium 1082Protozoa, Vairimorpha necatrix 1085Protozoa mitochondria 1110Proximal tubular cells, rat kidney 1162, 1163Pulmonary epithelial Type II cells 795Purple membranes 193Pyknotic thymocytes 690

RRabbit bone marrow cells 38Rabbit erythrocytes 946Rabbit mitochondria 1108Rabbit neurons 480Rabbit uterine cells (myocytes) 1152Radioactive labelling of Percoll 554

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Rat alveolar macrophage 780, 834Rat bone marrow cells 1046Rat brain capillary cells 170Rat brain glial progentior cells 1148Rat cerebral microvessels 1139Rat chondrocytes 629Rat dorsal root ganglia neurons 794Rat eosinophils 974Rat erythrocytes 151, 285, 945Rat granule 1117Rat kidney proximal tubular cells 1162, 1163Rat Leydig cells 31, 92, 993, 994,

995, 996, 997, 998,999, 1000

Rat liver 713Rat liver cells 20, 24, 33, 55,

71, 976, 977, 978,979, 980, 981, 982,

983, 984, 985Rat lysosomes 24, 1105, 1106Rat macrophages 1055, 1056, 1057, 1058Rat mast cells 9, 1060, 1061, 1062Rat mitochondria 1109Rat ovarian cells (granulosa-lutea cells) 1161Rat ovarian cells (thecal-interstitial cells) 1159Rat peroxisomes 53Rat placenta trophoblasts 1068Rat plasma membrane 33, 1089, 1090, 1091,

1092, 1093, 1094, 1095Rat stomach cells (brush cells) 1151Rat thymocytes 62, 1064, 1065, 1066Red blood cell density 765Red blood cell ghosts, rat 72Red blood cells 584, 764, 765Red blood cells, bullfrog 128, 349Red blood cells, human 55, 66, 77, 94,

108, 114, 140, 164,350, 462, 760, 761,

762, 763Red blood cells, monkeyparasite-infected 263Red blood cells, murine 163, 264Red blood cells, murine,parasite-infected 293Red blood cells, rat 151, 285Red blood cells, young and old 761Removal of tissue culturecontaminants 845, 874Renin granules 616, 631Resting B cells isolated on Percoll 769Reticulocyte mitochondria 784Reticulocyte, rat 833Retina cells, perch 1138Retinal cells, chick embryo 65, 158Retinal pigment epithelium, bovine 583

Rickettsia cowdria ruminantium 848Rickettsia prowazekii 825Rickettsia tsutsugamushi 299Rosettes, human T cell 127, 131, 173,

245, 254Rosettes, mouse spleen cells 160

SSaccharomyces cerevisiae, mutants 1149Sample loading techniques 288Sarcolemmal vesicles, rat aorta 116Sarcoma cells, ascites 557Sarcoma cells, mouse 126Scallop embryos (Pecten maximus) 669Scenedesmus obliquus 620Schistosoma mansoni cercariae 279, 364, 510Schistosomula 546, 581Sea urchin granule 1118Secretory cells, plant 865Secretory vesicles, bovineneurohypophysis 134Secretory vesicles, chromaffin cell 232Separation of bacteria from a co-culture 688Separation of bacteria from wastewater 632Serotonin granules, pig platelet 267Sertoli cells 838SEYF-a tumour cells, mouse 150Sheep plasma membrane 1097Sickle cells 935Skate plasma membrane 1099Skeletal muscle cells 680Soil microorganisms 247, 881Solutes, density effects 654Somatotrophs, rat anteriorpituitary gland 315, 883, 886Soybean nodules 708Sperm, human 648, 660Spermatids, mammalial 670Spermatogenic cells, rat 50, 335, 381Spermatozoa 662, 698, 778Spermatozoa, boar 213Spermatozoa, bovine 1024Spermatozoa, hamster 1025Spermatozoa human 4, 161, 423, 448,

466, 526, 673, 745,846, 853, 859, 861,

876, 890, 1001, 1002,1003, 1004, 1005, 1006,1007, 1008, 1009, 1010,1011, 1012, 1013, 1014,1015, 1016, 1017, 1018,1019, 1020, 1021, 1022,

1023Spermatozoa, macaque 1026Spermatozoa, mammalian 839

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Spermatozoa, motility 662Spermatozoa, rabbit 25Spermatozoa, rat 847Spermatozoa, X-bearing 679Spermiophages, turkey 1073Spheroplasts, Saccharomyces cerevisiae 565Spherulous cells, marine sponge 835Spinach leaf chloroplast 76, 88Spleen cell blasts, mouse 141, 144, 345Spleen cell fractionation 704, 799Spleen cells, chicken 228Spleen cells, murine 21, 142, 265, 286,

292, 592, 799, 860Spleen cells, murine T and B 314Spleen cells, rat 266, 276, 307Spleen cells, resting 542Spores, endomycorrhizal 182Spores, myxosoma 828Spores, slime mold 194Sporocysts 752Sporozoites 752, 877Sporozoites, Theileria annulata 258Sputum 643Stern cells 386Stomach cells (brush cells), rat 1151Stoll epithelial cells, human 1158Streptococcus cell walls 788Streptococcus pyogenes adhesion 731Streptococcus subpopulations 810Striated muscle cell nuclei 832Stromal cells, rat uterus 1144Subcellular somponents, rat liver 361, 422Subcellular fraction 597, 1134Subcellular fraction, human 727, 1069, 1133Subcellular fractionation ofmurine fibroblasts 702Subcellular fractionation 844Subcellular fractionation, canine liver 403Subprotoplasts 195Sugar beet leaf mitochondria 1121Sulphur content of bacterial cells,density changes 646Sunflower seed mitochondria 1120Suppressor cells 579Suppressor lymphocytes, human 44, 192, 436Surfactant protein D (lectin) 1153Synaptic mitochondria 657Synaptosomes 552, 641, 754, 774Synaptosomes, rat 753, 786, 870, 887,

888Syncytiotrophoblast plasma membrane 590Syncytiotrophoblasts, monkey placenta 1156Synovial tissue cells 241Syntrophic bacteria 688

Syntrophomonas wolfei 688Syphilis 593

TT cell precursors, marrow 737T cell rosettes, human 127, 131, 331, 340T-cells, human 421T-lymphocyte colony-forming cells 540T-lymphocytes 529, 550T-lymphocytes, human 261, 390, 501, 503,

504, 507Teratocarinoma cells, mouse 248Testicle cells, calf 21Testicle cells, mouse 31Testicle cells rat 50, 335Testicular intertubular cells, rat 649, 879Tetrahymena thermophila 401Thecal-interstitial cells, rat 1159Theileria annulata sporozoites 258Theileria-infected erythrocytes 730Thrombocyte-depleted lymphocytes 531Thymic dendritic cells 826Thymocytes 659, 690, 716Thymocytes, guinea pig 330Thymocytes, human 231, 602Thymocytes, human infant 342Thymocytes, murine 62, 265, 819, 1063Thymocytes, rat 62, 211, 221, 1064,

1065, 1066Thymus cells, B cell depleted 563Thyrocytes 488Tobacco leaf chloroplast 1122Tobacco leaf mitochondria 1121Torpedo california lipid vesicles 392Toxoplasma cysts 269Treponema pallidum 321, 481, 628Treponema pallidum, Nichols strain 593Trichinella spiralis 855Trophoblast basal layer cells 773Trophoblastic cells, human 39Trophoblasts, rat placenta 1068Trophozoites, Pneumocystis carinii 1155Trout erythrocytes 947Trypanosoma cruzi 400, 691Trypanosoma, African 371Tryponosoma brucei 756Tryponosome fractionation 756Tubules, rabbit kidney 206Tumor cell heterogenity 720Tumor cell quantitation in blood 554Tumor cell subpopulations 720, 757, 782, 816Tumor cells 554, 574, 655, 757Tumor cells, human 225, 816, 1070

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Tumor cells, mouse 225Tumor cells, rat 1070Turkey sperm mitochondria 1111Turkey spermiophages 1073

UUnit gravity sedimentation 250Uterine cells (stromal cells), rat 1144Uterine cells (myocytes), rabbit 1152

VVacuole, spheroplast 565Vacuoles, plant 181Vaginal epithelium, mouse 652Vairimorpha necatrix, protozoa 1085Veiled cells, canine 818Vesicles, Electrophorus membrane 189Viable and non-viable cells 557, 1070Viral particles 693Virus purification 570, 693Virus, African swine fever 570Virus, rice transitory yellowingvirus (RTYV) 1086Virus, Rubivirus (rubella virus) 1087Virus, Herpes simplex virus 56, 156

WWastewater analysis 632Wheat leaf mitochondria 1121

XX and Y sperm separation 648X-bearing spermatozoa 744Xanotrophores, goldfish 379Xenosomes 689

YYeast (Saccharomyces cerevisiae),mutants 1149Yeast cells 874Yolk sac cells 552

ZZooxanthellae 582Zymogen granules 605, 619, 775

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List of references

1. Parenchymal cell mass determinations in human parathyroid glands. Åkerström, G.,Johansson, H. et al., Presented at Societé International de Chirurgie, San Francisco (August 1979).

2. Density determinations of human parathyroid glands by density gradients. Åkerström, G.,Pertoft, H., Grimelius, L. et al., Acta Path. Microbiol. Scand. Sect. A. 87, 91–96 (1979).

3. Estimation of parathyroid parenchymal cell mass using density gradients. Åkerström, G.,Grimelius, L., Johansson, H. et al., Am. J. Pathol. 99, 685–694 (1980).

4. Washing and concentration of human semen by Percoll density gradients and its applicationto AIH. Iizuka, R. et al., Arch. Androl. 20, 117–124 (1988).

5. Rapid isolation of pancreatic islets from collagenase digested pancreas by sedimentationthrough Percoll at unit gravity. Buitrago, A., Gylfe, E., Henriksson, C. et al., Biochem.Biophys. Res. Commun. 79, 823–828 (1977).

6. Isolation and characterization of cells from rat adipose tissue developing into adipocytes.Björntorp, P., Karlsson, M., Pertoft, H. et al., J. Lipid Res. 19, 316–324 (1978).

7. Presence of alloreactive Ia antigens on murine intestine epithelial cells. Curman, B., Kämpe,O., Rask, L. et al., Scand. J. Immunol. 10, 11–15 (1979).

8. Separation of lymphoid cells, mast cells and macrophages on Percoll density gradient.Courtoy, R., Simar, L.J. and Delrez, M. Presented at a Meeting of the Belgian Society ofImmunology. (December 1978).

9. Isolation of rat peritoneal mast cells by centrifugation on density gradients of Percoll.Enerbäck, L. and Svensson, I. J. Immunol. Methods. 39, 135–145 (1980).

10. Distribution of Ia-antigen-like molecules on non-lymphoid tissues. Forsum, U., Klareskog, L.and Peterson, P.A. Scand. J. Immunol. 9, 343–349 (1979).

11. Separation of human lymphocytes on the basis of volume and density. Hutchins, D. andSteel, C.M. In Separation of Cells and Subcellular Elements (Peeters, H., Ed.) PergamonPress, Oxford and New York (1979)

12. Separation of chloroplasts from mitochondria utilising silica sol gradient centrifugation.Jackson, C., Dench, J.E., Halliwell, B. et al., Presented at The Wolffson Conference, Universityof Surrey (July 1978).

13. Simple analytical subcellular fractionation of liver biopsies with Percoll. Jenkins, W.J.,Clarkson, E., Milson, J. et al., Clin. Sci. 57, 29P (1979).

14. Coloured beads as density markers in isopycnic centrifugation. Kågedal, L. and Pertoft, H.Abstracts, 12th FEBS Meeting, Dresden (1978).

15. Separation of blood cells from healthy and leukemic donors in Percoll gradients. Rogge, H.,Christ, H., Grosshans, E. et al., Abstracts, 12th FEBS Meeting, Dresden (1978).

16. Long term growth in vitro of human T cell blasts with maintenance of specificity andfunction. Kurnick, J.T., Grönvik, K.-O., Kimura, A.K. et al., J. Immunol 122, 1255–1260 (1979).

17. Uptake and degradation of mast cell granules by mouse peritoneal macrophages. Lindahl, U.,Pertoft, H. and Seljelid, R. Biochem. J. 182, 189–193(1979).

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18. Purification and steroidogenic responses of isolated rat luteal cells. McNamara, B.C.,Booth, R. and Stansfield, D.A. Presented at International Union of Biochemistry MeetingToronto (1979).

19. Separation of lactotrophs from hyperplastic rat adenohypophysis using Percoll densitygradients. Milligan, J.V. Abstracts, Canad. Fed. Biol. Sci. Meeting, Vancouver (1979).

20. Isolation and characterization of hepatocytes and Küpffer cells. Page, D.T. and Garvey, J.S.J. Immunol. Methods 27, 159–173 (1979).

21. Isopycnic separation of cells and cell organelles by centrifugation in modified colloidal silicagradients. Pertoft, H. and Laurent, T.C. In Methods of Cell Separation Vol. 1.(Catsimpoolas, N., Ed.) Plenum Press, New York 25–65 (1977).

22. Density gradients prepared from colloidal silica particles coated with polyvinylpyrrolidone(Percoll). Pertoft, H., Laurent, T.C., Låås, T. et al., Anal. Biochem. 88, 271–282 (1978).

23. The viability of cells grown or centrifuged in a new density gradient medium, Percoll.Pertoft, H., Rubin, K., Kjellén, L. et al., Exp. Cell Res. 110, 449–457 (1977).

24. Heterogeneity of lysosomes originating from rat liver parenchymal cells. Metabolic relation-ship of subpopulations separated by density gradient centrifugation. Pertoft, H.,Wärmegård, B. and Höök, M. Biochem. J. 174, 309–317 (1978).

25. Characterization of rabbit sperm by equilibrium sedimentation in Percoll during frequentejaculation. Oshio, S., Kaneko, S. and Mohri, H. Arch. Androl. 17, 189–194 (1986).

26. A two-step procedure for the purification of hepatitis B surface antigen (HbsAg). Einarsson, M.,Kaplan, L. and Pertoft, H. Vox Sanguinis 41, 91–97 (1981).

27. The use of density gradients of Percoll for the separation of biological particles. Pertoft, H.,Laurent, T.C., Seljelid, R. et al., In Separation of Cells and Subcellular Elements, (Peeters,H., Ed.) Pergamon Press Oxford and New York 67–72 (1979).

28. Collection of dinoflagellates and other marine microalgae by centrifugation in densitygradients of a modified silica sol. Price, C.A., Reardon, E.M. and Guillard, R.R.L. Limnol.Oceangr. 123, 548–553 (1978).

29. Adhesion of rat hepatocytes to collagen. Rubin, K., Oldberg, Å., Höök, M. et al., Exp. CellRes. 117, 165–177 (1978).

30. Macrophage functional heterogeneity: evidence for different antibody-dependent effectorcell activities and expression of Fc-receptors among macrophage subpopulations. Serio, C.,Gandour, D.M and Walker, W.S. J. Reticuloendothelial Soc. 25, 197–216 (1979).

31. Rapid isolation of mouse Leydig cells by centrifugation in Percoll density gradients withcomplete retention of morphological and biochemical integrity. Schumacher, M., Schäfer, G.,Holstein, A.F. et al., FEBS LETT. 91, 333–338 (1978).

32. Epithelial rat liver cells have cell surface receptors recognizing a phosphorylated carbohydrateon lysosomal enzymes. Ullrich, K., Mersmann, G., Fleischer, M. et al., Hoppe-Seyler's Z.Physiol. Chem. 359, 1591–1598 (1978).

33. Specific bindning of rat liver plasma membranes by rat liver cells. Öbrink, B., Wärmegård, B.and Pertoft, H. Biochem. Biophys. Res. Commun. 77, 665–670 (1977).

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34. Liberation of a fibrogenic factor from human blood monocytes, ascites cells, culturedhistiocytes and transformed mouse macrophages by treatment with SiO2. Aalto, M.,Kulonen, E., Rönnemaa, T. et al., Scand. J. Clin. Lab. Invest. 39, 205–214 (1979).

35. Parenchymal cell mass determinations in human parathyroid glands and its application in amaterial of hyperparathyroidism. Åkerström, G., Grimelius, L., Johansson, H. et al.,World. J. Surg. 5, 555–563 (1981).

36. In vitro induction of self reactive T lymphocyte memory in cultures of syngeneic peanutagglutinin-negative mouse thymocytes and spleen cells. Born, W. and Wekerle, H.Immunobiol. 156, 243–244 (1979).

37. Characteristics of cultured brain capillaires. Bowman, P.D., Betz, A.L. and Goldstein, G.W.J. Cell Biol. 83, 95a (1979).

38. Characterization of bone marrow osteoprogenitor cell lines. Budenz, R.W. and Bernard, G.W.J. Cell Biol. 83, 32a (1979).

39. Isolation, characterization and cultivation of human trophoblastic cells. Calaminus, J.M.,Brüggen, J. and Sorg, C. Immunobiol. 156, 287 (1979).

40. Characterization of procoagulant activity produced by cultures of human monocytes andlymphocytes separated in colloidal silica-polyvinylpyrrolidone gradients. Giddings, J.C.,Piovella, F., Ricetti, M. et al., Clin. Lab. Haematol. 2, 121–128 (1980).

41. Purification of human T and B cells by a discontinuous density gradient of Percoll.Gutierrez, C., Bernabe, R.R., Vega, J. et al., J. Immunol. Methods 29, 57–63 (1979).

42. Production of Ficoll, Percoll and albumin gradients by the freeze-thaw method. Haff, L.A.Prep. Biochem. 9, 149–156 (1979).

43. Isolation of intact higher-plant mitochondria. Jackson, C. and Moore, A.L. Plant Organelles,Methodological Surveys (B) Biochemistr, Vol. 9, (Reid, E., Ed.) Ellis Horwood Ltd, Chichester,West Sussex, UK 1–12 (1979).

44. Human MLC activated suppressor cells - enrichment on discontinuous density gradients.Kabelitz, D., Fink, U. and Reichert, A. Immunobiol. 156, 218 (1979).

45. Physical chemical characterization of Percoll. I. Particle weight of the colloid. Laurent, T.C.,Pertoft, H. and Nordli, O. J. Colloid Interface Sci. 76, 124–132 (1980).

46. Physical chemical characterization of Percoll. II. Size and interaction of colloidal particles.Laurent, T.C., Ogston, AJ.G., Pertoft, H. et al., J. Colloid Interface Sci. 76, 133–141 (1980).

47. Physical chemical characterization of Percoll. III. Sodium binding. Laurent, T.C. andPertoft, H. J. Colloidal Interface Sci. 76, 142–145 (1980).

48. Peanut agglutinin. IV. A tool for studying human mononuclear cell differentiation. London, J.,Perrot, J.Y., Berrih, S. et al., Scand. J. Immunol. 9, 451–459 (1979).

49. Conversion of 4-hydroxyphenylpyruvic acid into homogentisic acid at the thylakoidmembrane of Lemna gibba. Löffelhardt, W. and Kindl, H. FEBS LETT. 104, 332–334 (1979).

50. Further purification of rat spermatogenic cells by density centrifugation. Meistrich, M.L.,Longtin, J.L. and Brock, W.A. J. Cell Biol. 83, 226a (1979).

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51. Protozoan parasite-induced proliferative response of primed T lymphocytes. Moedder, E.,Engers, H. and Louis, J. Immunobiol. 156, 205 (1979).

52. In vitro evidence suggests a direct action of adjuvants on myeloid precursor cells in the bonemarrow. Monner, D.A.L. and Mühlradt, P.F. Immunobiol. 156, 189–190 (1979).

53. Regulation of peroxisomal fatty acid oxidation. Osmundsen, H. and Neat, C.E.FEBS LETT. 107, 81–85 (1979).

54. Asymmetric distribution of arachidonic acid in the plasma membrane of human platelets. Adetermination using purified phospholipases and a rapid method for membrane isolation.Perret, B., Chap, H.J. and Douste-Blazy, L. Biochim. Biophys. Acta 556, 434–446 (1979).

55. Cell separations in a new density medium, Percoll. Pertoft, H., Hirtenstein, M. and Kågedal, L.Cell Populations, Methodological Surveys (B) Biochemistry Vol. 9. (Reid, E., Ed.)Ellis Horwood Ltd, Chichester, West Sussex, UK 67–80 (1979).

56. Purification of herpes simplex virus using Pecoll. Amersham Biosciences Fine Chemicals, Pertoft, H.Separation News 3 (1980).

57. Separation of human monocytes on density gradients of Percoll. Pertoft, H., Johnsson, A.,Wärmegård, B. et al., J. Immunol. Methods 33, 221–230 (1980).

58. Isolation of chloroplasts in silica-sol gradients. Price, C.A., Bartolf, M., Ortiz, W. et al.,Plant Organelles, Methodological Surveys (B) Biochemistry Vol. 9. (Reid, E., Ed.)Ellis Horwood Ltd, Chichester, West Sussex, UK 25–33 (1979).

59. Fractionation of subcellular components by centrifugation in Percoll density gradients.Pertoft, H. and Hirtenstein, M. Presented at The Wolfson Conference, University of Surrey,Guildford, UK (July 1978).

60. Harvesting of marine microalgae by centrifugation in density gradients of Percoll.Reardon, E.M., Price, C.A. and Guillard, R.R.L. Cell Populations, Methodological Surveys(B) Biochemistry Vol. 9. (Reid, E., Ed.) Ellis Horwood Ltd, Chichester, West Sussex, UK (1979).

61. Morphological and functional characterization of isolated effector cells responsible forhuman natural killer activity to fetal fibroblasts and to cultured cell line targets. Saksela, E.,Timonen, T., Ranki, A. et al., Immunological Rev. 44, 71–123 (1979).

62. A rapid method for the separation of large and small thymocytes from rats and mice.Salisbury, J.G., Graham, J.M. and Pasternak, C.A. J. Biochem. Biophys. Methods 1,341–347 (1979).

63. Separation of lymphocytes, polymorphonuclear leucocytes and lysosomes by densitygradient centrifugation. Seale, T.W. In Manual of Procedures for the Seminar onBiochemical Hematology, (Sunderman, F.W., Ed.) The Institute for Clinical Science Inc.,Philadelphia, PA 355–367 (1979).

64. Disaggregation and separation of rat liver cells. Seglen, P.O. Cell Populations, MethodologicalSurveys (B) Biochemistry Vol. 9. (Reid, E., Ed.) Ellis Horwood Ltd, Chichester, West Sussex,UK 25–46 (1979).

65. Separation of cell populations from embryonic chick neural retina. Sheffield, J.B., Lynch, M.and Pressman, D. J. Cell Biol. 83, 34a (1979).

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66. The effect of erythrocyte aging on some vitamin and mineral dependent enzymes. Spooner, R.J.,Percy, R.A. and Rumley, A.G. Clin. Biochem. 12, 289–290 (1979).

67. Fractionation, morphological and functional characterization of effector cells responsiblefor human natural killer activity against cell-line targets. Timonen, T., Saksela., E. Ranki, A.et al., Cell. Immunol. 48, 133–148 (1979).

68. Recognition of human urine a-N-acetylglucosaminidase by rat hepatocytes. Involvement ofreceptors specific for galactose, mannose-6-phosphate and mannose. Ullrich, K., Basner, R.,Gieselmann, V. et al., Biochem. J. 180, 413–419 (1979).

69. Discontinuous density gradient separation of human mononuclear leucocytes using Percollas gradient medium. Ullmer, A.J. and Flad, H.-D. J. Immunol. Methods 30, 1–10 (1979).

70. Isolation and surface labeling of murine polymorphonuclear neutrophils. Watt, S.M.,Burgess, A.W. and Metcalf, D. J. Cell Physiol. 100, 1–22 (1979).

71. Primary cultures of rat hepatocytes synthesize fibronectin. Voss, B., Allam, S., Rauterberg, J.et al., Biochem. Biophys. Res. Commun. 90, 1348–1354 (1979).

72. In vitro characterization of anti-tumor effector mechanisms in rats bearing spontaneous tumors.Zöller, M. and Matzku, S. Immunobiol. 156, 276 (1979).

73. Percoll methodology. Amersham Biosciences. Separation News 1 (1979).

74. Fractionation of blood cells. In Centrifugation: A Practical Approach. Jurd, R.D. andRickwood, D. (Rickwood, D., Ed.) Information Retrival Ltd, 1 Falconberg Court,London W1V 5FG, UK 143–152 (1978).

75. Choice of media for centrifugal separations. In Centrifugation: A Practical Approach. Jurd, R.D.and Rickwood, D. (Rickwood, D., Ed.) Information Retrieval Ltd, 1 Falconberg Court,London W1V 5FG, UK 15–31 (1978).

76. Post-translational transport into intact chloroplasts of a precursor to the small subunit ofribulose-1,5-bisphosphate carboxylase. Chua, N.-H. and Schmidt, G.W. Proc. Nat. Acad. Sci.USA 75, 6110–6114 (1978).

77. Human erythrocyte fractionation in Percoll density gradients. Rennie, C., Thompson, S.,Parker, A. et al., Clin. Chim. Acta 98, 119–125 (1979).

78. Isolation of a protein from the plasma membrane of adrenal medulla which binds tosecretory vesicles. Meyer, D. and Burger, M. J. Biol. Chem. 254, 9854–9859 (1979).

79. Techniques of preparative, zonal, and continuous flow ultracentrifugation.Beckman® Instruments Inc. Spinco division, USA, Griffith, O.M.

80. Cytophysical studies on living normal and neoplastic cells. Mateyko, G.M. and Kopac, M.J.Ann. N.Y. Acad. Sci. 105, 185–218 (1963).

81. The use of gradients of colloidal silica for the separation of cells and subcellular particles.Pertoft, H. and Laurent, T.C. Modern Separation Methods of Macromolecules and Particles Vol. 2.(Gerritsen, T., Ed.) Wiley Interscience, John Wiley & Sons, New York and London 71–90 (1968).

82. The separation of cells and subcellular particles by colloidal-silica density centrifugation.Wolff, D.A. In Methods in Cell Biology Vol. 10. (Prescott D.M., Ed.) Academic Press New Yorkand London 85–104 (1975).

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83. A rapid method for the separation of functional lymphoid cell populations of human andanimal origin on PVP-silica (Percoll) density gradients. Kurnick, J.T., Österberg, L.,Stegagno, M. et al., Scand. J. Immunol. 10, 563–573 (1979).

84. A novel reagent for the fluorometric assay of primary amines. Weigele, M., DeBarnardo, S.L.,Tengi, J.D. et al., J. Am. Chem. Soc. 94, 5927–5928 (1972).

85. Application of the Weichselbaum biuret reagent to the determinations of spinal fluid protein.Ditterbrandt, M. Am. J. Clin. Pathol. 18, 439–441 (1948).

86. Isolation of highly purified rat liver mitochondria for the study of the biotransformation of drugs.Blume, H. (Article in German). Archiv der Pharmazie 312, 561–572 (1979).

87. Subcellular distribution of liver copper in normal subjects, patients with primary biliarycirrhosis and Wilson's disease. Jenkins, W.J., Evans, S. and Epstein, O. Clin. Sci. 58, 14 (1979).

88. Isolation of intact chloroplasts from spinach leaf by centrifugation in gradients of modifiedsilica Percoll. Tahabe, T., Nishimuru, M. and Akazawa, T. Agric. Biol. Chem. 43,2137–2142 (1979).

89. Isolation and characterization of noradrenalin storage granules of bovine adrenal medulla.Terland, O., Flatmark T. and Kryvi, T. Biochim. Biophys. Acta 553, 460–468 (1979).

90. A rapid and sensitive method for the quantitation of microgram quantities of proteinutilising the principle of protein-dye bindning. Braford, M. Anal. Biochem. 72, 248–254 (1976).

91. A parapoxvirus isolated from nasal secretion of a calf with respiratory disease.Moreno-Lopéz, J. and Lif, I. Vet. Microbiol. 4, 85–88 (1979).

92. Isolation of rat mast cell granules with intact membranes. Krüger, P.G., Lagunoff, D. andWan, H. Exp. Cell Res. 129, 82–93 (1980).

93. Long term maintenance of HLA-D restricted T cells specific for soluble antigens. Kurnick, J.,Altevogt, P., Lindblom, J. et al., Scand. J. Immunol. 11, 131–136 (1980).

94. A rapid centrifugation step method for the separation of erythrocytes, granulocytes andmononuclear cells on continuous density gradients of Percoll. Segal, A., Fortuno, A. andHerd, T. J. Immunol. Methods 32, 209–214 (1980).

95. Isolation of blood monocytes by use of Percoll. Seljelid, R. and Pertoft, H. In Methods forStudying Mononuclear Phagocytes. (Edelson, P., Koren, H. and Adams, D.O., Eds.)Academic Press, London and New York (1980).

96. Isolation of human NK cells by density gradient centrifugation. Timonen, T. and Saksela, E.J. Immunol. Methods 36, 285–291 (1980).

97. Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silicagel (Percoll). Gärtner, I. Immunology 40, 133–136 (1980).

98. Separation of human bone marrow cells in density gradients of polyvinylpyrrolidone-coatedsilica gel (Percoll). Olofsson, T., Gärtner, I. and Olsson, I. Scand. J. Haematol. 24, 254–262 (1980).

99. Improved separation of human peripheral T-cells using PVP-coated colloidal silica particles(Percoll). Feucht, H.E., Hadam, M.R., Frank, F. et al., In Separation of Cells and SubcellularElements. (Peeters, H., Ed.) Pergamon Press, Oxford and New York 73–76 (1979).

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100. Surface markers of a purified peritoneal eosinophil population from Mesocestoides corti-infected BALB/c male mice. Hogarth, P.M., Cruise, K.M., McKenzie, I.F.C. et al., J. Immunol.124, 406–411 (1980).

101. Separation of human blood monocytes and lymphocytes on a continuous Percoll gradient.Gmelig-Meyling, F. and Waldmann, T.A. J. Immunol. Methods, 33, 19 (1980).

102. Collagenase - Percoll isolation and intraportal transplantation of pancreatic islets in the mouse.Henriksson, C. and Soome, A. Presented at Societé Internationale de Chirurgie, San Fransisco(August 1979).

103. Calcium uptake and release by skeletal-muscle mitochondria. Mickelson, J.R. and Marsh, B.B.Cell Calcium 1, 119–128 (1980).

104. Rapid separation of rat peritoneal mast cells with Percoll. Németh, A. and Rölich, P. Eur. J.Cell Biol. 20, 272–275 (1980).

105. Maximal steroidogenic capacity of mouse Leydig cells. Kinetic analysis and dependence onprotein kinase activation and cAMP accumulation. Schumacher, M., Schäfer, G.,Lichtenberg, V. et al., FEBS LETT. 107, 398–402 (1979).

106. Separation of mitochondria from contaminating subcellular structures utilizing silica solgradient centrifugation. Jackson C., Dench, J.E.., Hall, D.O. et al., Plant Physiol. 64,150–153 (1979).

107. Human parathyroid cells in vitro - the occurrence of an autonomous cell population inadenomas and uremic hyperplasias. Ljunghall, S., Åkerström, G. And Rudberg, C. In 15thEuropean Symposium on Calcified Tissues, Helsinki (1980), (Kaitila, I. and Penttinen, O., Eds.)Springer International, supplement to Calcified Tissue International 31, 88 (1980).

108. A Percoll gradient for the separation of malaria infected eryhrocytes from low parasitizedblood. Biochemical studies of malaria parasites in a Plasmodium vinckei/ NMRI-mousesystem. Kientsch, R., Engel, W.D., Ziegler, A. et al., Submitted to Mol. Biochem. Parasitol (1980).

109. A rapid method for isolation of purified, physiologically active chloroplasts, used to studythe intracellular distribution of amino acids in pea leaves. Mills, W.R. and Joy, K.W.Planta 148, 75–83 (1980).

110. Use of a novel rapid preparation of fat-cell plasma membranes employing Percoll to investigate the effects of insulin and adrenaline on membrane protein phosphorylation withinintact fat cells. Belsham, G. J., Denton, R.M. and Tanner, M.J.A. Biochem. J. 192, 457–467 (1980).

111. A simple and fast method for the isolation of basolateral plasma membranes from rat smallintestinal epithelial cells. Scalera, V., Storelli, C., Storelli-Joss, C. et al., Biochem. J. 186,177–181 (1980).

112. Induction of peroxisomal b-oxidation in rat liver by high fat diets. Neat, C.E., Thomassen, M.S.and Osmundsen, H. Biochem. J. 186, 369–371 (1980).

113. Isolation of bacteria from yogurt on gradients of Percoll generated in situ. Standard courseexperiment. Amersham Biosciences Fine Chemicals.

114. Binding of immunoglobulin classes to subpopulations of human red blood cells separated bydensity-gradient centrifugation. Alderman, E.M., Fudenberg, H.H. and Lovins, R.E.Blood 55, 817–822 (1980).

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115. Selected populations of alloreactive T cells contain helper T cells but lack ThId, an antigen-specific helper T cell required for dominant production of the T15 idiotype. Bottomly, K.and Janeway, C.A. Eur. J. Immunol. 11, 270–274 (1981).

116. A rapid method for the preparation of sarcolemmal vesicles from rat aorta, and the stimulationof calcium uptake into the vesicles by cyclic AMP-dependent protein kinase. Brockbank, K.J.and England, P.J. FEBS LETT. 122, 67–71 (1980).

117. Isolated pollen cultures and pollen dimorphism.Heberle-Bors, E. and Reinert, J.Naturwissenschaften 67, 311–312 (1980).

118. Rapid isolation of functionally intact pancreatic islets from mice and rats by Percoll gradientcentrifugation. Brunstedt, J. Diabete & Metabolisme 6, 87–89 (1980).

119. Preparation and surface labeling of murine eosinophils. Burgess, A.W., Cruise, K.M.,Mitchell, G.F. et al., Exp. Hematol. 8, 108–119 (1980).

120. Actinin-containing branched microvilli isolated from an ascites adenocarcinoma.Carraway, K.L. Huggins, J.W., Cerra, R.F. et al., Nature 285, 508–510 (1980).

121. The isolation of intact adrenal chromaffin granules using isotonic Percoll density gradients.Carty, S.E., Johnson, R.G. and Scarpa, A. Anal. Biochem. 106, 438–445 (1980).

122. Human cutaneous leishmania in a mouse macrophage line: propagation and isolation ofintracellular parasites. Chang, K.P. Science 209, 1240–1242 (1980).

123. Resolution of cardiac lysosomes on Percoll gradients. Chua, B.H.L., Lins, J.A. andMorgans, H.E. Fed. Proc. 39, 1603 (1980).

124. A simple and rapid method for the isolation of human monocytes. Cochrum, K., Hanes, D.,Fagan, G. et al., Transplant. Proc. 10, 867–870 (1978).

125. Simultaneous isolation of brush border and basolateral membrane from rabbit enterocytes.Colas, B. and Maroux, S. Biochim. Biophys. Acta 600, 406–420 (1980).

126. The applicability of freeze-thaw Percoll gradients to whole-cell isopycnic fractionations:preliminary results. Cooper, A.J. and Perry, S. J. Immunol. Methodes 37, 353–361 (1980).

127. Histocompatibility requirements for T cell help in specific in vitro antibody responses toinfluenza virus by human blood lymphocytes. Callard, R.E. and Smith, C.M.Eur. J. Immunol. 11, 206–212 (1981).

128. Density gradient separation of differentiating red blood cells. Dorn, A.R. and Broyles, R.H.Fed. Proc. 39, 1601 (1980).

129. Synchronization of cell division in microorganisms by Percoll gradients. Dwek, R.D.,Kobrin, L.H., Grossman, N. et al., J. Bacteriol. 144, 17–21 (1980).

130. Mechanism of action of mouse macrophages as anti-tumor effector cells: role of arginase.Farram, E. and Nelson, D.S. Cell. Immunol. 55, 283–293 (1980).

131. Efficient separation of human T lymphocytes from venous blood using PVP-coated colloidalsilica particles (Percoll). Feucht, H.E., Hadam, M.R., Frank, F. et al., J. Immunol. Methods38, 43–51 (1980).

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132. In vitro uptake of particles by lysosomes by means of microautophagy. Glaumann, H.,Ahlberg, J. and Marzella, L. Eur. J. Cell Biol. 22, 200 (1980).

133. The presence of latent acetylcholinesterase in purified secretory vesicles of adrenal medulla.Gratzl, M., Krieger-Brauer, H. and Ekerdt, R. Eur. J. Cell Biol. 22, 186 (1980).

134. Isolation and characterization of secretory vesicles from bovine neurohypophyses. Gratzl, M.,Torp-Pedersen, C., Dartt, D. et al., Hoppe-Seyler's Z. Physiol. Chem. 361, 1615–1628 (1980).

135. Energy-dependent uptake of cytoplasmically synthesized polypeptides by choloroplasts.Grossman, A., Bartlett, S. and Chua, N.-H. Nature 285, 625–628 (1980).

136. Failure of rabbit neutrophils to secrete endogenous pyrogen when stimulated with staphylococci.Hansson, D.F., Murphy, P.A. and Windle, B.E. J. Exp. Med. 151, 1360–1371 (1980).

137. Isolation of human monocytes on re-orienting gradients of Percoll. Hardin, J.A. and Downs, J.T.J. Immunol. Methodes 40, 1–6 (1981).

138. Purification and characterization of human lysosomes from EB-virus transformedlymphoblasts. Harms, E., Kartenbeck, J., Darai, G. et al., Exp. Cell Res. 131, 251–266 (1981).

139. Bactericidal activity of eosinophil peroxidase. Jong, E.C., Henderson, W.R. and Klebanoff, S.J.J. Immunol. 124, 1378–1382 (1980).

140. Isolation of the phagocytosis-inducing IgG-binding antigen on senescent somatic cells.Kay, M.M.B. Nature 289, 491–494 (1981).

141. Carrageenan stimulates populations of mouse B cells mostly nonoverlapping with thosestimulated with LPS or dextran sulfate. Kolb, J.-P.B., Quan P.C., Poupon, M.-F. et al.,Cell. Immunol. 57, 348–360 (1981).

142. Plasminogen activation by normal B lymphocytes, a function associated with the cell membrane.Maillard, J.L. and Favreau, C. J. Immunol. 126, 1126–1130 (1981).

143. Separation of membrane-bound-glutamyl transpeptidase from brush border transport andenzyme activities. Mamelok, R.D., Groth, D.F. and Prusiner, S.B. Biochemistry 19,2367–2373 (1980).

144. Immunoglobulin C-gene expression. I. The commitment to IgG subclass of secretory cells isdetermined by the quality of the nonspecific stimuli. Martinez-Alonso, C., Coutinho, A. andAugustin, A.A. Eur. J. Immunol. 10, 698–702 (1980).

145. In vitro uptake of particles by lysosomes. Marzella, L., Ahlberg, J. and Glaumann, HExp. Cell Res. 129, 460–466 (1980).

146. The preparation and purification of isolated rat corpus-luteum cells and their use in studyingthe relationship between cholesterol biosynthesis and the lutropin-stimulated formation ofprogesterone. McNamara, B.C., Cranna, C.E.G., Booth, R. et al., Biochem. J. 192, 559–567 (1980).

147. Purification of skeletal-muscle mitochondria by density-gradient centrifugation with Percoll.Mickelson, J.R., Greaser, M.L. and Marsh, B.B. Anal. Biochem. 109, 255–260 (1980).

148. Isolation and partial characterization of the plasma membrane of purified bovine neutrophils.Mottola, C., Gennaro, R., Marzullo, A. et al., Eur. J. Biochem. 111, 341–346 (1980).

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149. Evidence that specific oligosaccharides block early events necessary for the expression ofantigen-specific proliferation by human lymphocytes. Muchmore, A.V., Decker, J.M. andBlaese, R.M. J. Immunol. 125, 1306–1311 (1980).

150. Separation of tumor-seeking small lymphocytes and tumor cells using Percoll velocity gradients.Nethanel, T., Kinsky, R., Moav, N. et al., J. Immunol. Methods 41, 43–56 (1981).

151. A simple method for separation of uninfected erythrocytes from those infected withPlasmodium berghei and for isolation of artificially released parasites. Nillni, E.A.,Londner, M.V. and Spira, D.T. Z. Parasitenkd. 64, 279–284 (1981).

152. Synthesis of polypeptides of the chlorophyll-protein complexes in isolated chloroplasts ofEuglena gracilis. Ortiz, W. and Stutz, E. FEBS LETT. 116, 298–302 (1980).

153. Effect of cyclosporin A on T-dependent and T-independent immunoglobulin synthesis in vitro.Paavonen, T. and Häyry, P. Nature 287, 542–544 (1980).

154. Cell division and giant cell formation in Küpffer cell cultures. Pulford, K. and Souhami, R.L.Clin. Exp. Immunol. 42, 67–76 (1980).

155. Human megakaryocytes. I. Characterization of the membrane and cytoplasmic componentsof isolated marrow megakaryocytes. Rabellino, E.M., Nachmann, R.L., Williams, N. et al.,J. Exp. Med. 149, 1273–1287 (1979).

156. Separation of herpes simplex virus virions and nucleocapsids on Percoll gradients.Svennerholm, B., Vahlne, A., Jeansson, S. et al., J. Virol. Methods 1, 303–309 (1980).

157. Studies on megakarayocytes: isolation from rat and guinea pig and incorporation of5-hydroxytryptamine. Seitz, R. and Wesemann, W. Eur. J. Cell Biol. 21, 183–187 (1980).

158. Cells isolated from the embryonic neural retina differ in behavior in vitro and membrane structure.Sheffield, J.B., Pressman, D. and Lynch, M. Science 209, 1043–1045 (1980).

159. Regulation of heparan sulphate metabolism by adenosine 3',5'-cyclic monophosphate inhepatocytes in culture. Sudhakaran, P.R., Sinn, W. and von Figura, K. Biochem, J. 192,395–402 (1980).

160. Clonal analysis of B lymphocyte subpopulations separated on the basis of Lyb surface antigens.Teale, J.M., Pike, B.L., Craig, J. et al., Cell. Immunol. 55, 272–282 (1980).

161. Isolation of spermatozoa in vaginal contents by centrifugation in colloidal silica gradient.Takatoria, T. and Sasaki, T. Forensic Science International 15, 61–65 (1980).

162. Natural killer cells are responsible for the interferon production induced in humanlymphocytes by tumor cell contact. Timonen, T., Saksela, E., Virtanen, I. et al.,Eur. J. Immunol. 10, 422–427 (1980).

163. Plasmodium yoelii and Plasmodium berghei: isolation of infected erythrocytes from bloodby colloidal silica gradient centrifugation. Tosta, C.E., Sedegah, M., Henderson, D.C. et al.,Exp. Parasitol. 50, 7–15 (1980).

164. A new density gradient system for the separation of human red blood cells. Vettore, L.,de Matteis, M.C. and Zampini, P. Amer. J. Hematol. 8, 291–297 (1980).

165. Selective enrichment of embryogenic microspore populations. Wernicke, W., Harms, C.T.,Lörz, H. et al., Naturwissenschaften 65, 540–541(1978).

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166. ADP-ribosylation of nuclear proteins on normal lymphocytes and in low grade malignantnon-Hodgkin lymphoma cells. Wielckens, K., Garbrecht, M., Kittler, M. et al., Eur. J. Biochem.104, 279–287 (1980).

167. Establishment of pure giunea pig epidermal melanocyte cultures utilizing density gradientcentrifugation. Wilkins, L.M., Wachs, S. and Szabo, G. In vitro 16, 229 (1980).

168. Isolation of hatching gland cells from the teleost, Oryzias latipes, by centrifugation throughPercoll. Yoshizaki, N., Sackers, R.J., Schoots, A.F.M et al., J. Exp. Zool. 213, 427–429 (1980).

169. Density gradient fractionation of effector cells in human natural cell-mediated cytotoxicity.Bloom, E.T. Cell. Immunol. 61, 231–244 (1981).

170. Primary culture of capillary endothelium from rat brain. Bowman, P.D., Betz, A.L., Ar, D et al.,In vitro 17, 353–362 (1981).

171. Isolation of purified rat Leydig cells using continuous Percoll gradients. Browning, J.Y.,D´Agata, R. and Grotjan, H.E. Endocrinology 109, 667–669 (1981).

172. Separation of newborn rat epidermal cells on discontinuous isokinetic gradients of Percoll.Brysk, M.M., Snider, J.M. and Smith, E.B. J. Invest. Dermatol. 77, 205–209 (1981).

173. Unusual phenotype and function of an expanded subpopulation of T cells in patients withhaemopoietic disorders. Callard, R.E, Smith, C.M., Worman, C. et al., Clin. Exp. Immunol.43, 497–505 (1981).

174. Morphology of human peripheral blood mononuclear cells isolated by isopycnic centrifugationon a Percoll gradient. Cambier, P.H. and Biemond, I. Ultramicroscopy 5, 99 (1980).

175. Human peripheral T cell subsets separated on discontinuous density gradients. Canonica, G.W.,Sjöberg, O. and Kabelitz, D. Immunobiology 157, 210–211 (1980).

176. Effect of lutropin and cycloheximide on lutropin receptors and cyclic AMP production inLeydig tumor cells in vitro. Dix, C.J. and Cooke, B.A. Biochem. J. 196, 713–719 (1981).

177. Isolation of mast cells from murine peritoneal cell populations by velocity sedimentation atunit gravity. Dullens, H.F.J., Van Basten, Ch., De Weger, R.A. et al., J. Immunol. Methods40, 367–372 (1981).

178. Isolated rat parietal cells: comparison of binding and functional response to cholinergic drugs.Ecknauer, R., Johnson, L.R. and Rosenfeld, G.C. Fresenius Z. Anal. Chem 301, 197 (1980).

179. Combination of techniques for concentration and identification of microfilariae fromperipheral blood. Feldmeier, H., Bienzle, U. and Schuh, D. Trans. Roy. Soc. Tropical Med. Hyg.75, 251–253 (1981).

180. Three-step isolation of human blood monocytes using discontinuous density gradients of Percoll.Fluks, A.J. J. Immunol. Methods 41, 225–233 (1981).

181. Intracellular localization of capsaicin and its analogues in Capsicum fruit. II. The vacoule asthe intracellular accumulation site of capsaicinoid in the protoplast of Capsicum fruit.Fujiwake, H., Suzuki, T. and Iwai, K. Plant cell Physiol. 21, 1023–1030 (1980).

182. Media for density gradient extraction of endomycorrhizal spores. Furlan, V., Bartschi, H.and Fortin, J.-A. Trans. Br. Mycol. Soc. 75, 336–338 (1980).

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183. Human B cell activation in vitro: augmentation and suppression by monocytes of theimmunoglobulin production induced by various B cell stimulants. Gmelig-Meyling, F. andWaldmann, T.A. J. Immunol. 126, 529–537 (1981).

184. Purification of human peripheral blood colony forming cells (CFUc). Goldberg, J., McGuire, L.A.,Dock, N.L. et al., Exp. Hematol. 8, 1086–1093 (1980).

185. Cyanide-insensitive and cyanide-sensitive O2 uptake in wheat. I. Gradient-purified mitochondria.Goldsterin, A.H., Anderson, J.O. and McDaniel, R.G. Plant Physiol. 66, 488–493 (1980).

186. Interaction of chromaffin granules with plasma membranes mediated by Ca2+ and Mg2+ -ATPusing self-generating gradients of Percoll. von Grafenstein, H. and Neumann, E. FEBS Lett.123, 238–240 (1981).

187. Separation of haemopoietic cells for biochemical investigation. Preparation of erythroid andmyeloid cells from human and laboratory-animal bone marrow and the separation oferythroblasts according to their state of maturation. Harrison, F.L., Beswick, T.M. andChesterton, C.J. Biochem. J. 194, 789–796 (1981).

188. Autophagy of metabolically inert substances injected into fibroblasts in culture. Hendil, K.B.Exp. Cell Res. 135, 157–166 (1981).

189. Specific reaction rate of acetylcholine receptor controlled ion translocation: a comparison ofmeasurements with membrane vesicles and with muscle cells. Hess, G.P., Aoshima, A.,Cash, D.J. et al., Proc. Natl. Acad. Sci. USA 78, 1361–1365 (1981).

190. A rapid method for purification of human granulocytes using Percoll. A comparison withdextran sedimentation. Hjort, R., Jonsson, A.-K. and Vretblad, P. J. Immunol. Methods 43,95–101 (1981).

191. Restricted concanavalin A redistribution on the branched microvilli of an ascites tumor subline.Huggins, J.W., Trenbeath, T.P., Yeltman, D.R. et al., Exp. Cells Res. 127, 31–46 (1980).

192. Enrichment of MLR-activated human suppressor cells by discontinuous density-gradientcentrifugation. Kabelitz, D., Fink, U., Aigrain, Y. et al., Immunobiology 158, 403–415 (1981).

193. Polarigrapich assay of the binding of certain "probe" molecules to illuminatedbacteriorhodopsin sheets. Kell, D.B. and Griffiths, A.M. Photobiochem. Photobiophys. 2,105–110 (1981).

194. Purification of Dictyostelium discoideum spores by centrifugation in Percoll density gradientswith retention of morpohological and biochemical integrity. Killick, K.A. Anal. Biochem.114, 46–52 (1981).

195. Isolation of subprotoplasts for genetic manipulation studies. In Advances in Protoplast Research.Szeged, Hungary. Lörz, H. and Potrykus, I. Proceedings of the 5th International ProtoplastSymposium, July 9-14 (1979).

196. Morphological characterization of a cell population responsible for natural killer activity.Luini, W., Boraschi, D., Alberti, S. et al., Immunology 43, 663–668 (1981).

197. Transcription in isolated wheat nuclei. I. Isolation of nuclei and elimination of endogeneousribonuclease activity. Luthe, D.S. and Quatrano, R.S. Plant Physol. 65, 305–308 (1980).

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198. Assessment of antibody mediated cytolysis of adult cardiocytes isolated by centrifugation ina continuous gradient of Percoll in patients with acute myocarditis. Maisch, B.,Trostel-Soeder, R., Berg, P.A. et al., J. Immunol. Methods 44, 159–169 (1981).

199. The preparation of isolated rat corpus-luetum cells and their use in identifying the site ofaction of lutropin. McNamara, B.C., Booth, R. and Stansfield, D.A. Biochem. Soc. Trans.8, 305 (1981).

200. Effects of high fat diets on hepatic fatty acid oxidation in the rat. Isolation of rat liverperoxisomes by vertical rotor centrifugation by using a self-generated, iso-osmotic, Percollgradient. Neat. C.E., Thomassen, M.S. and Osmundsen, H. Biochem. J. 196, 149–159 (1981).

201. Measurements of cell density of three freshwater phytoplankters by density gradientcentrifugation. Oliver, R.L., Kinnear, A.J. and Ganf, G.G. Limnol. Oceanogr. 26, 285–294 (1981).

202. A rapid method for the isolation of intact glycosomes from Trypanosoma brucei by Percollgradient centrifugation in a vertical rotor. Opperdoes, F.R. Mol. Biochem. Parasitol. 3,181–186 (1981).

203. Effect of cyclosporin A on in vitro proliferative activity and immunoglobulin synthesis ofisolated human lymphoid cell subpopulations. Paavonen, T., Järveläinen, H., Kontianinen, S.et al., Clin. Exp. Immunol. 43, 342–350 (1981).

204. Oligodendroglial glycerophospholipid synthesis: incorporation of radioactive precursorsinto ethanolamine glycerophospholipids by calf oligodendroglia prepared by a Percollprocedure and maintained in suspension culture. Pleasure, D., Hardy, M., Johnson, G. et al.,J. Neurochem. 37, 452–460 (1981).

205. A rapid one-step method for the isolation of bacteroids from root nodules of soybeanplants, utilizing self-generating Percoll gradients. Reibach, P.H., Mask, P.L. and Streeter, J.G.Can. J. Microbiol. 27, 491–495 (1981).

206. A rapid method for fractionation of nephron components using a non-toxin isosmoticgradient material (Percoll). Richardson, J.C.W. and Simmons, N.L. J. Physiol. 310, 1P–2P (1981).

207. T lymphocytes in colostrum and peripheral blood differ in their capacity to form thermostableE-rosettes. Richie, E.R., Steinmetz, K.D., Meistrich, M.L. et al., J. Immunol. 125,2344–2346 (1980).

208. Transglutaminase as a marker for subsets of murine macrophages. Schroff, G., Neumann, C.and Sorg, C. Eur. J. Immunol. 11, 637–642 (1981).

209. Isolation of photosynthetically active protoplasts and chloroplasts from Arabidopsis thaliana.Somerville, C.R., Somerville, S.C. and Ogren W.L. Plant Sci. Lett. 21, 89–96 (1981).

210. Separation of cytotoxin leukocyte populations of human peripheral blood and colostrum onPVP-silica (Percoll) density gradients. Steinmetz, K.D., Kohl, S. and Richie, E.R. J.Immunol. Methods 42, 157–170 (1981).

211. Glucocorticoid-induced cell-size changes and nuclear fragility in rat thymocytes. Thomas, N.and Bell, P.A. Mol. Cell. Endocrinol. 22, 71–84 (1981).

212. Functional properties of isopycnic fractions of human peripheral blood monocytes. Tice, D.G.,Goldberg, J. and Nelson, D.A. J. Reticuloendotel. Soc. 29, 459–464 (1981).

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213. A new separation method of subcellular fractions of boar spermatozoa. Töpfer-Petersen, E.and Schill, W.-B. Andrologia 13, 174–176 (1981).

214. Separation and properties of prestalk and prespore cells of Dictyostelium discoideum.Tsang, A. and Bradbury, J.M. Exp. Cell Res. 132, 433–441 (1981).

215. Initial characterization of monoclonal antibodies against human monocytes. Ugolini, V.,Nunez, G., Smith, R.G. et al., Proc. Natl. Acad. Sci. USA 77, 6764–6768 (1980).

216. Isolation of chromaffin cell plasma membranes on polycationic beads. Van der Meulen, J.A.,Emerson, D.M. and Grinstein, S. Biochim. Biophys. Acta 643, 601–615 (1981).

217. Comparaison entre deux gradients de density pour separer les CFC. (Article in French.)Vannier, J.P., Monconduit, M. and Piguet, H. Biomedicine 33, 236–239 (1980).

218. The generation of specific cytotoxin responses by human lymphocytes antigen and activitiesprovided by a lymphokine supernatant. Warren, H.S. Scand. J. Immunol. 14, 71–76 (1981).

219. Turnover of the surface proteins and the receptor for serum asialoglycoproteins in primarycultures of rat hepatocytes. Warren, R. and Doyle, D. J. Biol. Chem. 256, 1346–1355 (1981).

220. Primary culture of adrenal medullary chromaffin cells in a chemically defined medium.Wilson, S.P. and Viveros, O.H. Exp. Cell Res. 133, 159–169 (1981).

221. Separation of the dead cell fraction from X-irradiated rat thymocyte suspensions by densitygradient centrifugation. Yamada, T. and Ohyama, H. Int. J. Radiat. Biol. 37, 695–699 (1980).

222. Sustained growth in primary culture of normal mammary epithelial cells embedded incollagen gels. Yang, J., Richards, J., Guzman, R. et al., Proc. Natl. Acad. Sci. USA 77,2088–2092 (1980).

223. Isolation of peroxisomes from rat liver using sucrose and Percoll gradients. Appelkvist, E.L.,Brunk, U. and Dallner, G. J. Biochem. Biophys. Methods 5, 203–217 (1981).

224. Primary and secondary autologous mixed lymphocyte interactions in mice occur via antigensencoded by genes in the I region of the major histocompatibility complex. Bocchieri, M.H.,Wolos, J.A. and Smith, J.B. Cell. Immunol. 65, 142–151 (1981).

225. A rapid method for the isolation of metastasizing tumour cells from internal organs with thehelp of isopycnic density-gradient centrifugation in Percoll. Bosslet, K., Ruffmann, R.,Altevogt, P. et al., Br.J. Cancer 44, 356–362 (1981).

226. Separation of human peripheral blood monocytes on continuous density gradients ofpolyvinypyrrolidone coated silica gel (Percoll). Brandslund, I., Möler Rasmussen, J. Fisker, D.et al., J. Immunol. Methods 48, 199–211 (1982).

227. The structure and composition of protein bodies purified from barley endosperm by silicasol density gradients. Cameron-Mills, V. Carlsberg Res. Commun. 45, 557–576 (1980).

228. Cytotoxicity to allogeneic cells in the chicken. Specific cytotoxin T cells and macrophages inthe spleens of agammaglobulinemic and normal alloimmune chickens. Chi, D., Blyznak, N.,Kimura, A. et al., Cell. Immunol. 64, 246–257 (1981).

229. Human B-lymphocyte subpopulations. Dagg, M.K. and Levitt, D. Clin. Immunol. Immunopathol.21, 39–49 (1981).

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230. Large scale production of human monocytes by leukapheresis. Feige, U., Knop, J. and Sorg, C.Immunobiol. 157, 217 (1980).

231. Separation of human thymocytes at different stages of maturation by centrifugation on adiscontinuous gradient of colloidal silica gel. Goust, J.-M. and Perry, L.R. Thymus 3,25–33 (1981).

232. Latent acetylcholinesterase in secretory vesicles isolated from adrenal medulla. Gratzl, M.,Krieger-Brauer, H. and Ekerdt, R. Biochim. Biophys. Acta 649, 355–366 (1981).

233. Rates of protein synthesis through the cell cycle of Saccharomyces cerevisiae. Gulløv, K.,Friis, J. and Bonven, B. Exp. Cell Res. 136, 295–304 (1981).

234. Isolation of brush-border membrane from the rabbit descending colon epithelium.Partical characterization of a unique K+-activated ATPase. Gustin, M.C. and Goodman, D.B.P.J. Biol. Chem. 226, 10651–10656 (1981).

235. The densities of bacetial cell walls. Humphries, M., Wilkinson, A.E., Edwards, B. et al.,Biochem. Soc. Trans. 9, 436–437 (1981).

236. Separation and concentration of murine hematopoietic stem cells (CFUs) using a combinationof density gradient sedimentation and counterflow centrifugal elutriation. Inoue, T.,Carsten, A-L., Cronkite, E.P. et al., Exp. Hematol. 9, 563–572 (1981).

237. A simple method for the isolation of basolateral plasma membrane vesicles from rat kidney cortex.Inui, K.-I., Okano, T., Takano, M. et al., Biochim. Biophys. Acta 647, 150–154 (1981).

238. A rapid one-step method for isolation of human granulocytes from whole blood.Jepsen. L.V. and Skottun. T. Scand. J. Clin. Lab. Invest. 42, 235–238 (1982).

239. Percoll: An effective medium for cleaning microsporidian spores. Jouvenaz. D.P.J. Invertebrate Pathol. 37, 319 (1981).

240. A method for quantitation of protein in the presence of Percoll. Khan, M.N., Khan, R.J. andPosner, B.I. Anal. Biochem. 117, 108–112 (1981).

241. Appearance of anti-HLA-DR-reactive cells in normal and rheumatoid synovial tissue.Klareskog, L., Forsum, U., Malmnnäs Tjernlund, U. et al., Scand. J. Immunol. 14,183–192 (1981).

242. Fractionation of arginase-loaded erythrocyte ghosts with Percoll density gradients.Kruse, C.A. and Popjak, G. Biochem. Biophys. Res. Commun. 102, 258–264 (1981).

243. Human large granular lymphocytes: Spontaneous and interferon-boosted NK activityagainst adherent and nonadherent tumor cell lines. De Landazuri, M.O., Lopez-Botet, M.,Timonen, T. et al., J. Immunol. 127, 1380–1381 (1981).

244. Long term culture of bovine oligodendroglia isolated with a Percoll gradient. Lisak, R.P.,Pleasure, D.E., Silberberg, D.H. et al., Brain Res. 223, 107–122 (1981).

245. A subset of human natural killer cells isolated and characterized by monoclonal antibodies.Lohmeyer, J., Rieber, P. Feucht, H. et al., Eur. J. Immunol. 11, 997–1001 (1981).

246. Conidiobolus obscurus in arable soil: a method for extracting and counting azygospores.MacDonald, R.M. and Spokes, J.R. Soil. Biol. Biochem. 13, 551–554 (1981).

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247. Separation of non-filamentous microorganisms from soil by density gradient centrifugationin Percoll. Martin, N.J. and MacDonald, R.M. J. Appl. Bacteriol. 51, 243–251 (1981).

248. Reversible and irreversible stages in the transition of cell surface marker during thedifferentiation of pluripotent teratocarcinoma cells induced with retinoic acid. Ogiso, Y.,Kume, A., Nishimune, Y. et al., Exp. Cell Res. 137, 365–372 (1982).

249. Regulation of protein phosphorylation by inotropic agents in isolated rat myocardial cells.Onorato, J.J. and Rudolph, S.A. J. Biol. Chem. 256, 10697–10703 (1981).

250. Rapid large scale fractionation of lymphoid cells by velocity sedimentation at unit gravity,using Percoll as separation medium. Opstelten, D., Dennen, G.J., Bos, L. et al.,J. Immunol. Methods 48, 189–198 (1982).

251. Lymphocyte surface markers in sheep blood and lymph. Outteridge, P.M., Fahey, K.J. andLee, C.S. Austral. J. Exp. Biol. Med. 59, 143–155 (1981).

252. Natural killer (NK) cell acitivity in the rat. Reynolds, C.W., Timonen, T. and Herberman, R.B.J. Immunol. 127, 282–287 (1981).

253. Simultaneous isolation of bovine eosinophils and neutrophils on gradients of Percoll.Riding, G.A. and Willadsen, P. J. Immunol. Methods 46, 113–119 (1981).

254. Human autologous rosettes. Further characterization: markers and functions.Rucheton, M., Caraux., Rey, A. et al., Cell. Immunol. 64, 312–323 (1981).

255. Sodium gradient- and sodium plus potassium gradient dependent L-glutamate uptake inrenal basolateral membrane vesicles. Sacktor, B., Rosenbloom, I.L., Liang, C.T. et al.,J. Membrane Biol. 60, 63–71 (1981).

256. Purification of mature microsporidian spores by isodensity equilibrium centrifugation.Sato, R. and Watanabe, H. J. Sericult. Sci. Japan 49, 512–516 (1980).

257. Natural killer activity of gut mucosal lymphoid cells in mice. Tagliabue, A., Luini, W.,Soldateschi, D. et al., Eur. J. Immunol. 11, 919–922 (1981).

258. Preliminary observations on the density of sporozoites of Theileria annulata. Walker, A.R.and McKellar, S.B. In Advances in the Control of Theileriosis. (Irvin, A. et al., Eds.)Martinus Nijhoff Publishers, The Hague 125–126 (1981).

259. Micropinocytic ingestion of glycosylated albumin by isolated microvessels: possible role inpathogensis of diabetic microangiopathy. Williams, S.K., Devenny, J.J. and Bitensky, W.W.Proc. Natl. Acad. Sci. USA 78, 2393–2397 (1981).

260. Variation in Escherichia coli buoyant density measured in Percoll gradients. Woldringh C.L.,Binnerts, J.S. and Mans, A. J. Bacteriol. 148, 58–63 (1981).

261. A two-step procedure for obtaining normal peripheral blood T lymphocytes using continuousequilibrium density gradient centrifugation on Percoll. Ali, F.M.K., May, A., McLaren, G.D.et al., J. Immunol. Methods 49, 185–191 (1982).

262. Monoclonal antibodies reactive with human myeloid leukaemia cells. Ball. E.D. and Fanger, M.W.Clin. Exp. Immunol. 48, 655–665 (1982).

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263. Altered expression of Plasmodium knowlesi variant antigen on the erythrocyte membrane insplenectomized Rhesus monkeys. Barnwell, J.W, Howard, R.J. and Miller, L.H. J. Immunol.128, 224–226 (1982).

264. Homeostatic removal of senescent murine erythrocytes by splenic macrophages. Bennett, G.D.and Kay, M.M.B. Exp. Hematol. 9, 297–307 (1981).

265. Lympho-stromal interactions in the thymus: medullary thymocytes react with I-A determinantson autochthonous thymic stimulator cells. Born, W. and Wekerle, H. Eur. J. Immunol. 12,51–59 (1982).

266. Rat NK cells active against lymphoma and sarcoma tumor cells are probably identical.Brooks, C.G., Flannery, G.R., Cantrell, D.A. et al., J. Immunol. 128, 913–919 (1982).

267. Serotonin transport in isolated platelet granules. Coupling to the electrochemical proton gradient.Carty, S.E., Johnson, R.G. and Scarpa, A. J. Biol. Chem. 256, 11244–11250 (1981).

268. Monocyte responsiveness to chemotactic stimuli is a property of a subpopulation of cellsthat can respond to multiple chemoattractants. Cianciolo, G.J. and Snyderman, R.J. Clin. Invest. 67, 60–68 (1981).

269. Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissueby continuous density-gradient centrifugation. Cornelissen, A.W.C.A., Overdulve, J.P. andHoederboom, J.M. Parasitol. 83, 103–108 (1981).

270. Use of Percoll density gradient centrifugation for preparing isolated rat hepatocytes havinglong-term viability. Dalet, C., Fehlmann, M. and Debey, P. Anal. Biochem. 122, 119–123 (1982).

271. Preparation and purification of lymphocytes from the epithelium and lamina propria ofmurine small intestine. Davies, M.D.J. and Parrott, D.M.V. Gut 22, 481–488 (1981).

272. Enrichment of hematopoietic progenitor cells from human bone marrow on Percoll densitygradients. Frickhofen, N., Heit, W. and Heimpel, H. Blut 44, 101–105 (1982).

273. Separation of epidermal cells by density gradient centrifugation on a continuous colloidalsilica (Percoll) gradient. Goldenhersh, M.A., Good, R.A., Sarkar, N.H. et al.,Anal. Biochem. 119, 246–252 (1982).

274. Separation of murine mononuclear phagocytes by density gradients of Percoll. Hester, R.B.and Walker, W.S. In Methods for Studying Mononuclear Phagocytes. (Adams, D.O.,Edelson, P.J., Koren, H.S., Eds.) Academic Press, New York and London 195–200 (1981).

275. Removal of Percoll from microsomal vesicles by gel filtration on Sephacryl S-1000 Superfine.Hjorth, R. and Pertoft, H. Biochim. Biophys. Acta 688, 1–4 (1982).

276, Autoimmune effector cells, II. Transfer of experimental allergic encephalomyelitis with asubset of T lymphocytes. Holda, J.H. and Swanborg, R.H. Eur. J. Immunol. 12, 453–455 (1982).

277. Lymphocyte transformation induced by autologous cells. XII. Quantitative and qualitativedifferences between human autologous and allogeneic reactive T lymphocytes. Kozak, R.W.,Moody, C.E., Staiano-Coico, L. et al., J. Immunol. 128, 1723–1727 (1982).

278. The separation of cell populations using monoclonal antibodies attached to Sepharose.de Kretzer, T.A., Bodmer, J.G. and Bodmer, W.F. Tissue Antigens 16, 317–325 (1980).

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279. Schistosoma mansoni: rapid isolation and purification of schistosomula of different developmentalstages by centrifugation on discontinuous density gradients of Percoll. Lazdins, J.K., Stein, M.J.,David, J.R. et al., Exp. Parasitol. 53, 39–44 (1982).

280. Higher order metaphase chromosome structure: evidence for metalloprotein interactions.Lewis, C.D. and Laemmli, U.K. Cell 29, 171–181 (1982).

281. Enrichment of vital adult cardiac muscle cells by continuous silica sol gradient centrifugation.Maisch, B. Basic Res. Cardiol. 76, 622–629 (1981).

282. Adenylcyclase-stimulating activity of enzymatically modified choriogonadotropin using ratLeydig cells purified by Percoll density gradient centrifugation. Merz, W.E. and Sessler, M.Acta Endocrinol. 96 Suppl. 83–84 (1981).

283. Expression ability of Ia antigens on T cell subsets defined by monoclonal antibodies onpokeweed mitogen stimulation in early human life. Miyawaki, T., Yachie, A., Nagaoki, T.et al., J. Immunol. 128, 11–15 (1982).

284. An improved technique for the isolation of lymphocytes from small volumes of peripheralmouse blood. Mizobe, F., Martial, E., Colby-Germinario, S. et al., J. Immunol. Methods 48,269–279 (1982).

285. Deformability and osmotic fragility of phenyl-hydrazine-injected rat erythrocytes fractionatedby Percoll density gradients. Nagasawa, T. Jap. J. Physiol. 32, 161–170 (1982).

286. A general stem cell system and its use for isolating stem cells by Percoll gradient centrifugationand centrifugal elutriation. Nijhof, W. Wierenga, P.K. and Goldwasser, E. Exp. Hematol.9 Suppl. 9, 176 (1981).

287. Sulfation of MHPG and some other substrates by human platelet phenolsulfotransferase, PST.Pennings, E.J.M., Van Brussel, J.L., Zanen, J.G. et al., In Phenolsulfotransferase in MentalHealth Research, (Sandler, M. and Usdin, E., Eds.) Macmillan Publishers Ltd., London andBasingstoke 29–43 (1981).

288. Isopycnic separation of cells by centrifugation in Percoll gradients. A. Liss, Inc. Pertoft, H.and Laurent, T.C. Presented at Proceedings of the Special FEBS Meeting, Athens,25–29 April (1982).

289. Sedimentation of cells in colloidal silica (Percoll). Pertoft, H. and Laurent, T.C. In CellSeparation: Methods and Selected Application. (Pretlow, T.G. and Pretlow, T.P., Eds.) In press.

290. A method for the purification of single A, B and D cells and for the isolation of coupledcells from isolated rat islets. Pipeleers, D.G. and Pipeleers-Marichal, M.A. Diabetologia 20,654–663 (1981).

291. Isolation and characterization of plasma membranes from Krebs II ascite cells using Percollgradient. Record, M., Bes, J.-C., Chap, H. et al., Biochim. Biophys. Acta 688, 57–65 (1982).

292. T-T cell interactions during cytotoxic T cell responses. IV. Murine lymphoid dendritic cellsare powerful stimulators for helper T lymphocytes.Röllinghoff, M., Pfizenmaier, K. andWagner, H. Eur. J. Immunol. 12, 337–342 (1982).

293. The use of Percoll gradients, elutriator rotor elution, and mithramycin staining for the isolationand identification of intraerythrocytic stages of Plasmodium berghei. Rüssmann,L., Jung, A.and Heidrich, H.-G. Z. Parasitenkd. 66, 273–280 (1982).

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294. The separation of cell organelles using Percoll gradients (Article in German). Sautter, C.,Kollmannsberger, E. and Hock, B. GIT Fachz. Lab. 26, 550–555 (1982).

295. Extraction of living meiofauna from marine sediments by centrifugation in a silica sol-sorbitolmixture. Schwinghamer, P. Canad. J. Fish. Aquat. 38, 476–478 (1981).

296. Changes in density of mitochondria and glyoxysomes from Neurospora crassa: a re-evaluationutilizing silica sol gradient centrifugation. Schwitzguébel, J.P., Møller, I.M. and Palmer, J.M.J. Gen. Microbiol. 126, 289–295 (1981).

297. Human eosinophils and parasitic diseases. II. Characterization of two cell fractions isolatedat different densities. De Simone, C., Donelli, G., Meli, D. et al., Clin. Exp. Immunol. 48,249–255 (1982).

298. Peripheral and integral proteins of human blood platelet membranes: actinin is not identicalto glycoprotein III. Sixma, J.J., Schiphorst, M.E., Verhoeckx, C. et al., Biochim, Biophys.Acta 704, 333–344 (1982).

299. Purification of Rickettsia tsutsugamushi by Percoll density gradient centrigufation.Tamura, A., Urakami, H. and Tsuruhara, T. Microbiol. Immunol. 26, 321–328 (1982).

300. Umbilical cord blood-derived suppressor cells of the human natural killer cell activity areinhibited by interferon. Tarkkanen, J. and Saksela, E. Scand. J. Immunol. 15, 149–157 (1982).

301. Tissue and subcellular localization of enzymes of arginine metabolism in Pisum sativum.Taylor, A.A. and Stewart, G.R. Biochem. Biophys. Res. Commun. 101, 1281–1289 (1981).

302. Human tumour antigens defined by cytotoxicity and proliferative responses of culturedlymphoid cells. Vose, B.M. and Bonnard. Nature 296, 359–361 (1982).

303. Is Percoll innocuous to cells? Wakefield, J. St. J., Gale, J.S., Berridge, M.V. et al., Biochem. J.202, 795–797 (1982).

304. Human eosinophil lysophospholipase: the sole protein component of Charcot-Leyden crystals.Weller, P.F., Bach, D. and Austen, K.F. J. Immunol. 128, 1346–1349 (1982).

305. Subcellular localization of O2-generating enzyme in guinea pig polymorphonuclear leukocytes;Fractionation of subcellular particles by using a Percoll density gradient. Yamaguchi, T.,Sato, K., Shimada, K. et al., J. Biochem. 91, 31–40 (1982).

306. A rapid method for the separation of rat pancreatic islets from collagenase-digested pancreasusing Percoll. Yamamoto, T., Asano, T, Mori, A. et al., Endocrinol. Japon. 28, 563–567 (1981).

307. Natural killer cells do not belong to the recirculating lymphocyte population. Zöller, M.,Bellgrau, D., Axberg, I. et al., Scand. J. Immunol. 15, 159–167 (1982).

308. Separation and characterization of neoplastic cell subpopulations if a transplantable ratacinar carcinoma. Becich, M.J. and Reddy, J.K. Cancer Res. 42, 3729–3740 (1982).

309. Primary culture of microvascular endothelial cells from bovine retina: Selective growth usingfibronectin coated substrate and plasma derived serum. Bowman, P.D., Betz, A.L. andGoldstein, G.W. In vitro 18, 626–632 (1982).

310. The heterogeneity of Leydig cells from mouse and rat testes - evidence for a Leydig cell cycle?Cooke, B.A., Magee-Brown, R., Golding, M. et al., Int. J. Andrology 4, 355–366 (1981).

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311. Isolation of large number of fully viable human neutrophils: A preparative technique usingPercoll density gradient centrifugation. Dooley, D.C., Simpson, J.F. and Meryman, H.T.Exp. Haematol. 10, 591–599 (1982).

312. Separation of epidermal cells by density centrifugation: a new technique for studies onnormal and pathological differentiation. Fischer, S.M., Nelson, K.D.G., Reiners, Jr. J.J. et al.,J. Cutaneous Path. 9, 43, 49 (1982).

313. Isolation of rat Leydig cells density gradient centrifugation. Gale, J.S., Wakefield, St. J. andFord, H.C. J. Endocr. 92, 293–302 (1982).

314. Alkaline phosphatase activity as a membrane marker for activated B cells. Garcia-Rozas, C.,Plaza, A., Diaz-Espada, F. et al., J. Immunol. 129, 52–55 (1982).

315. A procedure for the purification of somatotrophs isolated from rat anterior pituitary glandsusing Percoll density gradients. Hall, M., Howell, S.L., Schulster. D. et al., J. Endocr. 94,257–266 (1982).

316. The isolation and functional activity of polymorphonuclear leukocytes and lymphocytesseparated from whole blood on a single Percoll density gradient. Harbeck, R.J., Hoffman, A.A.,Redecker, S. et al., Clin. Immunol. and Immunopath. 23, 682–690 (1982).

317. A simplified method for assessing PHA induced stimulation of rat peripheral blood lymphocytes.Keller, S.E., Schleifer, S. J., McKegney, F.P. et al., J. Immunol. Methods 51, 287–291 (1982).

318. Internalization of insulin into rat liver Golgi elements. Evidence for vesicle heterogeneityand path of intracellular processing. Khan, M.N., Posner, B.I., Khan, R.J. et al., J.Biol. Chem.257, 5969–5976 (1982).

319. The purification of gametocytes of Plasmodium falciparum and P. yoelii nigeriensis bycolloidal silica (Percoll) gradient centrifugation. Knight, A. and Sinden, R.E. Trans. RoyalSoc. Trop. Med. Hygiene 76, 503–509 (1982).

320. Concentration of Plasmodium falciparum-infected erythrocytes by density gradient centrifugationin Percoll. Kramer, K.J., Kan, S.C. and Siddiqui, W.A. J. Parasitol. 68, 336–337 (1982).

321. Identification of Treponema pallidum antigens: Comparison with a nonpathogenictreponeme. Lukehart, S.A., Baker-Zander, S.A. and Gubish, Jr., E.R. J. Immunol. 129,833–838 (1982).

322. Slow passive diffusion of orthophosphate between intact isolated chloroplasts and suspendingmedium. Mourioux, G. and Douce, R. Plant Physiol. 67, 470–473 (1981).

323. Human granular lymphocytes and natural killing: ultrastructural studies of strontium-induceddegranulation. Neighbour, P.A., Huberman, H.S. and Kress, Y. Eur. J. Immunol. 12,588–595 (1982).

324. Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll.Neuburger, M., Journet, E.-P., Bligny, R. et al., Arch. Biochem. Biophys. 217, 312–323 (1982).

325. Preparation of epithelial and stromal cell fractions from immature rat prostatic tissue usingPercoll gradients. Orlowski, J., Bird, C.E. and Clark, A.F. J. Androl. 3, 232–240 (1982).

326. Presence of cyclic nucleotide-Ca2+ independent protein kinase in bovine brain coated vesicles.Pauloin, A., Bernier, I. and Jollés, P. Nature 298, 574–576 (1982).

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327. A procedure for the rapid preparation of mitochondria from rat liver. Reinhart, P.H.,Taylor, W.M. and Bygrave, F.L. Biochem. J. 204, 731–735 (1982).

328. Natural killer cell activity in the rat. Analysis of effector cell morphology and effects ofinterferon on natural killer cell function in the athymic (nude) rat. Reynolds, C.W.,Timonen, T.T., Holden, H.T. et al., Eur. J. Immunol. 12, 577–582 (1982).

329. Oxygen intermediates are triggered early in the cytolytic pathway of human NK cells.Roder, J.C., Helfand, S.L., Werkmeister, J. et al., Nature 298, 569–572 (1982).

330. Studies on thymocyte subpopulations in guinea pigs. Differences in cell volume andprofilerative ability in vitro of two populations separated by density gradients centrifugationwith Percoll. Sandberg, G. and Kölare, S. Med. Biol. 60, 132–138 (1982).

331. Antigen specificity and frequency of autologous and allogeneic helper T cells in the in vitroproduction of antibody against influenza virus by human blood lymphocytes. Smith, C.M.and Callard, R.E. Eur. J. Immunol. 12, 558–563 (1982).

332. Measurement of edema in the nervous system. Use of Percoll density gradients for determinationof specific gravity in cerebral cortex and white matter under normal conditions and inexperimental cytotoxin brain edema. Tengvar, Ch., Forssén, D., Olsson, Y. et al.,Acta Neuropat. 57, 143–150 (1982).

333. Isolation of human and rat natural killer cells. Timonen, T., Reynolds, C.W., Ortaldo, J.R.et al., J. Immunol. Meth. 51, 269–277 (1982).

334. Prenatal diagnosis of X-linked diseases. Bernstein, R.E. Clin. Gen. 18, 147–150 (1980).

335. Distribution and synthesis of histone 1 subfractions during spermatogenesis in the rat.Bucci, L.R., Brock, W.A. and Meistrich, M.L. Exp. Cell Res. 140, 111–118 (1982).

336. Percoll density gradient centrifugation. Fumarola, D., Antonaci, S., Jirillo, E. et al.,La Ricerca in Clin. E. in Lab. 12, 485–492 (1982).

337. Antigen presentation by human B cells: T cell proliferation induced by Epstein Barr virus Blymphoblastoid cells. Issekutz, T., Chu, E. and Geha. R. J. Immunol. 129, 1446–1450 (1982).

338. Inhibition of autologous mixed lymphocyte reaction by aggregated IgG molecules. Kabelitz, D.,Canonica, G.W., Sjöberg, O. et al., Eur. J. Immunol. 12, 687–691 (1982).

339. Trehalase from dormant spores of Dictyostelium discoideum isolated by Percoll densitygradient centrifugation. Killick, K.A. 8th Int. Spore Conf., Sporulation Germination.(Levison, H., Ed.) Woods Hole, MA 294–297 (1981).

340. In vitro influenza virus-specific antibody production in man: antigen-specific and HLA-restrictedinduction of helper activity mediated by cloned human T lymphocytes. Lamb, J.R., Woody, J.N.,Hartzman, R.J. et al., J. Immunol. 129, 1465–1470 (1982).

341. Regulation of human natural killing. II. Protective effect of interferon on NK cells fromsuppressing by PGE. Leung, K.H. and Koren, H.S. J. Immunol. 129, 1742–1747 (1982).

342. Distribution of terminal deoxynucleotidyl transferase and purine degradative and syntheticenzymes in subpopulations of human thymocytes. Ma, D.D.F., Sylwestrowicz, T.A. et al.,J. Immunol. 129, 1430–1435 (1982).

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343. Factors, which can influence b-oxidation by peroxisomes isolated from livers of clofibratetreated rats. Some properties of peroxisomal fractions isolated in a self-generated Percollgradient by vertical rotor centrifugation. Osmundsen, H. Int. J. Biochem. 14, 905–914 (1982).

344. Influence of a lymphokine fraction containing eosinophil stimulation promoter (ESP) onoxidative and degranulation responses of murine eosinophils. Rand, T.H. and Colley, D.G.Cell. Immunol. 71, 334–345 (1982).

345. H-2-restricted T-B cell interactions involved in polyspecific B cell response mediated bysoluble antigen. Ratcliffe, M.J.H. and Julius, M.H. Eur. J. Immunol. 12, 634–641 (1982).

346. A monoclonal antibody against a surface antigen shared by human large granularlymphocytes and granulocytes. Rumpold, H., Kraft, D., Obexer, G. et al., J. Immunol. 129,1458–1464 (1982).

347. Inhibition of basophil histamine release by anti-inflammatory steroids. II. Studies on themechanism of action. Schleimer, R.P., MacGlashan, Jr., D.W., Gillespie, E. et al.,J. Immunol. 129, 1632–1636 (1982).

348. Discrepancy in expression ability of Tac antigen and Ia determinants defined by monoclonalantibodies on activated or cultured cord blood T Lymphocytes. Yokoi, T., Miyawaki, T.,Yachie, A. et al., J. Immunol. 129, 1441–1445 (1982).

349. Erythrocyte differentiation during the metamorphic hemaglobin switch of Rana catesbeiana.Dorn, A.R. and Broyles, R.H. Proc. Natl. Acad. Sci. USA 79, 5592–5596 (1982).

350. Human erythrocyte separation according to age on a discontinuous Percoll density gradient.Salvo, G., Caprari, P., Samoggia, P. et al., Clin. Chim. Acta. 122, 293–300 (1982).

351. Characteristics of human large granular lymphocytes and relationship to natural killer andK cells. Timonen, T., Ortaldo, J.R. and Herberman, R.B. J. Exp. 153, 569–582 (1981).

352. Increased number of IgG Fc receptors on monocyte-enriched peripheral blood leucocytesfrom patients with rheumatoid arthritis. Rasmussen, J.M., Brandslund, I., Rasmussen, G.G.et al., Scand. J. Immunol. 16, 279–285 (1982).

353. The deleterious effect of mechanical dissociation of rat testes on the functional activity andpurification of Leydig cells using Percoll gradients. Aldred, L.F. and Cooke, B.A.Int. J. Androl. 5, 191–195 (1982).

354. Recycling of the hepatocyte asialoglycoprotein receptor does not require delivery of ligandto lysosomes. Baenziger, J.U. and Fiete, D. J. Biol. Chem. 257, 6007–6009 (1982).

355. Surface ultrastructural changes of Percoll separated splenic lymphocytes from syngeneic andallogeneic mice with transplanted ascites fibrosarcoma tumour. Basu, S., Chaudhuri, S. andRoy, B. Ind. J. Exp. Biol. 20, 277–281 (1982).

356. Both DR and MT class II HLA molecules may restrict proliferative T-lymphocyte responsesto antigen. Berle, E.J. and Thorsby, E. Scand. J. Immunol. 16, 543–547 (1982).

357. HLA-D/DR restriction of Langerhans cell-dependent antigen activation of T-lymphocytes.Berle, E.J., Braathen, L.R. and Thorsby. E. Scand. J. Immunol. 16, 549–552 (1982).

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358. Interferon production and natural cytotoxicity by human lymphocytes fractionated byPercoll density gradient centrifugation. Bloom, E.T. and Takasugi, M. In NK Cells andOther Natural Effector Cells. (Herberman, R.B., Ed.) Academic Press 675–681 (1982).

359. Mechanism of cell-mediated cytotoxicity at the single cell level. Bradley, T.P. and Bonavida, B.J. Immunol. 129, 2260–2265 (1982).

360. Analysis of buoyant density of canine peripheral blood leukocytes with PVP-silica (Percoll)density gradients. Buurman, W.A., Vegt, P.A., Groenwegen, G. et al., Vet. Immunol.Immunopath. 3, 547–556 (1982).

361. Separation of subcellular components from rat liver in Percoll density gradient. Chandoga, J.,Krizko. J. and Taragelova, E. Biologia 37, 815–823 (1982).

362. Separation of amniotic fluid cell types in primary culture by Percoll density gradient centrifugation.Cousineau, J., Potier, M., Dallaire, L. et al., Prenatal Diagnosis 2, 241–249 (1982).

363. The differential effects of human leukocytic pyrogen/lymphocyte-activating factor, T cellgrowth factor, and interferon on human natural killer activity. Dempsey. R.A., Dinarello, C.A.,Mier, J.W. et al., J. Immunol. 129, 2504–2510 (1982).

364. Schistosoma mansoni surface antigen defined by a rat monoclonal IgG2a. Dissous, C,Grzych, J.-M. and Capron, A. J. Immunol. 129, 2232–2234 (1982).

365. Remarks on the differentiation of lysosomes from cultured human fibroblasts by silicagradient centrifugation. Domsch, C. and Mersmann, G. Exp. Cell Res. 142, 482–485 (1982).

366. Purification of human blood monocytes by hypotonic density gradient centrifugation in Percoll.Feige, U., Overwien, B. and Sorg, C. J. Immunol. Methods 54, 309–315 (1982).

367. Isolation of mouse primordial germ cells. de Felici, M and McLaren, A. Exp. Cell Res. 142,476–482 (1982).

368. Isopycnic separation of Escherichia coli cultures possessing colonization factor antigen I.Giesa, F.R., Zajac, I. Bartus, H.F. et al., J. Clin. Microbiol. 15, 1074–1076 (1982).

369. A single-step centrifugation method for separation of granulocytes and mononuclear cellsfrom blood using discontinuous density gradient of Percoll. Giudicelli, J., Philip, P.J.M.,Delque, P. et al., J. Immunol. Methods 54, 43–46 (1982).

370. Enriched fraction of viable neurons from rabbit dorsal root ganglia (DRG), obtained byPercoll density gradient centrifugation. Goldenberg, S.S.S. and de Boni, U. In vitro 18, 313 (1982).

371. Isopycnic isolation of African trypanosomes on Percoll gradients formed in situ. Grab, D.J.and Bwayo, J.J. Acta Tropica 39, 363–366 (1982).

372. Inhibition of human natural killing by heterologous and monoclonal antibodies. Hiserodt, J.C.,Britvan, L.J. and Targan, S.R. J. Immunol. 129, 2248–2254 (1982).

373. Anti-Ia-reactive cells in the urinary tract of man, guinea pig, rat and mouse. Hjelm, E.,Forsum, U. and Klareskog, L. Scand. J. Immunol. 16, 531–538 (1982).

374. Isolation of the intracellular symbionts and partial characterizations of their RNA species ofthe elder aphid. Acrythosiphon magnoliae. Ishikawa, H. Comp. Biochem. Physiol. 72,239–247 (1982).

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375. Mechanisms of human cell-mediated cytotoxicity. III. Dependence of natural killing onmicrotubule and microfilament integrity. Katz, P., Zaytoun, A.M. and Lee, Jr, J.H.J. Immunol. 129, 2816–2825 (1982).

376. Human B cell differentiation. III. Enhancing effect of monoclonal anti-immunoglobulin Dantibody on pokeweed mitogen-induced plasma cell differentiation. Kuritani, T. andCooper, M.D. J. Immunol. 129, 2490–2495 (1982).

377. Characterization of porcine granulosa cells by isopycnic gradient centrifugation.Lahteenmaki, P., Lobo, R., Marrs, R.P. et al., Biol. Reprod. 27, 633–640 (1982).

378. Isolation of spinach leaf peroxisomes in 0.25 molar sucrose solution by Percoll density gradientcentrifugation. Liang, Z., Yu, C. and Huang, A.H.C. Plant Physiol. 70, 1210–1212 (1982).

379. Isolation of xanthophores from the goldfish (Carassius auratus L.). Lo, S.J., Grabowski, S.M.,Lynch, T.J. et al., In vitro 18, 356–360 (1982).

380. Purification of human melanocytes by monoclonal antibody combined with Percoll gradients.Marko, O., Houghton, A.N. and Eisinger, M. Exp. Cell. Res. 142, 309–315 (1982).

381. Purification of rat spermatogenic cells and preliminary biochemical analysis of these cells.Meistrich, M.L., Longtin, J., Brock, W.A. et al., Biol. Reprod. 25, 1065–1077 (1981).

382. Some functional and morphological characteristics of an acutely dispersed purified cellsuspension of rat lactotrophs prepared with Percoll. Milligan, J.V., McComb, D.J., Ryan, N.et al., Can. J. Physiol. Pharmacol. 60, 1450–1458 (1982).

383. Preparation of avocado mitochondria using self generated Percoll density gradients andchanges in buoyant density during ripening. Moreau, F. and Romani, R. Plant Physiol. 70,1380–1384 (1982).

384. Malate oxidation and cyanide-insensitive respiration in avocado mitochondria during theclimacteric cycle. Moreau, F. and Romani, R. Plant Physiol. 70, 1385–1390 (1982).

385. Immunomodulatory effects of amphotericin B on cellular cytotoxicity of normal humanlymphocytes. Nair, M.P.N. and Schwartz, S.A. Cell. Immunol. 70, 287–300 (1982).

386. A regenerating stem cell system and its use for isolation stem cells by centrifugal elutriationand Percoll-gradient centrifugation: The isolation of CFU-E. Nijhof, W., Wierenga, H.P.K.and Goldwasser, E. Int. Soc. Exp. Haem. 39–47 (1982).

387. Receptor-mediated endocytosis of transferrin and the uptake of Fe in K562 cells: Identificationof a non-lysosomal acidic compartment. van Renswoude, J., Bridges, K.R., Harford, J.B. et al.,Proc. Natl. Acad. Sci. 79, 6186–6190 (1982).

388. Cytotoxic antibodies to natural killer cells in systemic lupus erythematosus. Rook, A.H.,Tsokos, G.C., Quinnan, Jr., G.V. et al., Clin. Immunol. Immunopath. 24, 179–185 (1982).

389. Cyclosporin receptors on human lymphocytes. Ryffel, B., Götz, U. and Heuberger, B.J. Immunol. 129, 1978–1982 (1982).

390. Human T cells clones specific for tetanus toxoid: characterization of antigen specificity and HLArestriction. Schmitt, C., Ballet, J.-J., Agrapart, M. et al., Eur. J. Immunol. 12, 849–854 (1982).

391. Corticosteroids inhibit murine macrophage Ia expression and interleukin 1 production.Snyder, D.S. and Unanue, E.R. J. Immunol. 129, 1803–1805 (1982).

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392. Investigation of liposomes and vesicles reconstituted with acetylcholine receptor employingPercoll density gradient centrifugation. Spillecke, F. and Neumann, E. Symposium onNeuroreceptors, Berlin, Neuroreceptors 243–252 (1981).

393. Defective natural cytotoxicity in patients with cancer; normal number of effector cells butdecreased recycling capacity in patients with advanced disease. Steinhauer, E.H., Doyle, A.T.,Reed, J. et al., J. Immunol. 129, 2255–2259 (1982).

394. Nuclear buoyant density determination and the purification and characterization of wild-typeNeurospora nuclei using Percoll density gradients. Talbot, K.J. and Russell, P.J.Plant Physiol. 70, 704–708 (1982).

395. Central role of T lymphocytes in specific recognition of tumor antigens in 51Cr-leukocyteadherence inhibition. Tsang, P.H., Holland, J.F. and Bekesi, J.G. Cell. Immunol. 73,365–375 (1982).

396. Use of Percoll in the isolation and purification of rabbit small intestinal brush border membranes.Yakymyshyn, L.M., Walker, K. and Thomson, A.B.R. Biochim. Biophys. Acta 690,269–281 (1982).

397. Natural and antibody-dependent killer cells in the thymus. Zöller, M., Andrighetto, G. andHeyman, B. Eur. J. Immunol. 12, 914–921 (1982).

398. Normally occurring inhibitory cells for natural killer cell activity. Zöller, M. and Wigzell, H.Cell. Immunol. 74, 14–26 (1982).

399. Characterization of effector cells mediation IgG and IgM antibody-dependent cellular cytotoxicity.Zöller, M., Andrighetto, G.C., Heyman, B. et al., Scand. J. Immunol. 17, 19–27 (1983).

400. A method for isolating Trypanosoma cruzi amastigotes from spleen and liver using two-stepdiscontinuous gradient centrifugation. Abrahamsohn, I.A., Katzin, A.M. and Milder, R.V.J. Parasitol. 69, 437–439 (1983).

401. Highly purified micro- and macronuclei from Tetrahymena thermophila isolated by Percollgradients. Allen, S.L., White, T.C., Langmore, J.P. et al., J. Protozool. 30, 21–30 (1983).

402. Monitoring microbial numbers in food by density centrifugation. Basel, R.M., Richter, E.R.and Banwart, G.J. Appl. Environmental Microbiol. 45, 1156–1159 (1983).

403. Evaluation of preformed Percoll and reorientating sucrose density gradient centrifugationfor the analytical subcellular fractionation of dog liver. Batt, R.M. and Mann, L.C. Res. Vet. Sci.34, 272–279 (1983).

404. Increase in NK cell number and turnover rate during acute viral infection. Biron, C.A.,Turgiss, L.R. and Welsh, R.M. J. Immunol. 131, 1539–1545 (1983).

405. Increase cell buoyant densities of protein overproducing Escherichia coli cells. Cheng, Y.-S.E.Biochem, Biophys. Res. Comm. 111, 104–111 (1983).

406. Subfractionation of rat liver Golgi apparatus: Separation of enzyme activities involved in thebiosynthesis of the phosphomannosyl recognition marker in lysosomal enzymes. Deutscher, S.L.,Creek, K.E., Merion, M. et al., Proc. Natl. Acad. Sci. 80, 3938–3942 (1983).

407. A new density gradient technique for age-separation of human erythrocytes and reticulocytes.Ellory, J.C. and Wolowyk, M.W. J. Physiol. 295, 9–10 (1979).

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408. Endothelial cells are a site of uptake and degradation of hyaluronic acid in the liver.Eriksson, S., Fraser, J. and Laurent, T.C. et al., Exp. Cell Res. 144, 223–228 (1983).

409. Separation and purification of protoplast types from Commelina communis L. leaf epidermis.Fitzsimons, P.J. and Weyers, J.D.B. J. Exp. Bot. 34, 55–66 (1983).

410. Specific lysis of Plasmodium yoelii infected mouse erythrocytes with antibody and complement.Gabriel, J. and Berzins, K. Clin. Exp. Immunol. 52, 129–134 (1983).

411. Separation on Percoll density gradients of cells derived from malignant ascites of mice.Hamburger, A.W., Dunn, F.E. and Tencer, K.L. J.N.C.I. 70, 157–160 (1983).

412. Natural killer (NK) cells as a responder to interleukin 2 (IL 2). II. IL 2-induced interferonproduction. Handa, K., Suzuki, R., Matsui, H. et al., J. Immunol. 130, 988–992 (1983).

413. Long-term culture of oligodendrocytes isolated from rat corpus callosum by Percoll densitygradient. Hirayama, M., Silberberg, D.H. Lisak, R.P. et al., J. Neuropath. Exp. Neurol. 42,16–28 (1983).

414. Direct transfer of coliphage labmda DNA from Escherichia coli to cellular slime moldDictyostelium discoideum. Ishikawa, A. and Ikeda, H. Gene 21, 211–216 (1983).

415. Mechanisms of citrate oxidation by Percoll-purified mitochondria from potato tuber.Journet, E.-P. and Douce, R. Plant Physiol. 72, 802–808 (1983).

416. B cells as antigen-presenting cells: the requirement for B cell activation. Kakiuchi, T.,Chesnut, W. and Grey, H.M. J. Immunol. 131, 109–114 (1983).

417. A single centrifugation step method for the simultaneous separation of different leukocyteswith special reference to basophilic leukocytes. Kauffman, H.F., Levering, P.R. and de Vries, K.J. Immunol. Methods 57, 1–7 (1983).

418. Purification of porcine granulosa cells by continuous Percoll gradient. Kolena, J., Kiss, A.and Channing, C.P. Experientia 39, 908–909 (1983).

419. Gangliotriaosylceramide (asialo GM2), a glycosphingolipid marker for cell lines derivedfrom patients with Hodgkin's disease. Kniep, B., Mooner, D.A., Burrichter, H. et al.,J. Immunol. 131, 1591–1594 (1983).

420. Human B cell differentiation. IV. Effect of monoclonal anti-immunoglobulin M and Dantibodies on B cell proliferation and differentiation induced by T cell factors. Kuritani, T.and Cooper, M.D. J. Immunol. 131, 1306–1311 (1983).

421. In vitro selective expansion of allergen specific T cells from atopic patients. Lanzavecchia, A.,Santini, P., Maggi, E. et al., Clin. Exp. Immunol. 52, 21–28 (1983).

422. Antigenic and immunogenic components in rat liver. Lautenschlager, I., Nyman, N.,Vänänen, H. et al., Scand. J. Immunol. 17, 61–68 (1983).

423. Isolation of motile spermatozoa by density gradient centrifugation in Percoll. Lessley, B.A.and Garner, D.L. Gamete Res. 7, 49–61 (1983).

424. Enzyme activities in rabbit, guinea pig, rat and mouse blood cells after separation on adiscontinuous Percoll gradient. Lindena, J., Sommerfeld, U., Höpfel, C. et al., Enzyme 29,229–238 (1983).

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425. Enzyme activities blood cells of man and dogs after separation of a discontinuous Percollgradient. Lindena, J., Sommerfeld, U., Höpfel, C. and et al., Enzyme 29, 100–108 (1983).

426. Evidence that peroxisomal acyl-CoA synthetase is located at the cytoplasmic side of theperoxisomal membrane. Mannaerts, G.P., van Veldhoven, P., van Broekhoven, A. et al.,Biochem. J. 204, 17–23 (1983).

427. Antibody-secreting cell precursor frequencies among the sheep erythrocyte-binding cells ofnon-immune mice. Martinez-Maza, O., Fehniger, T.E. and Ashman, R.F. Scand. J. Immunol.17, 251–261 (1983).

428. Uptake by enterocytes and subsequent translocation to internal organs, e.g., the thymus, ofPercoll microspheres administered per os to suckling mice. Matsuno, K., Schaffner, T.,Gerber, H.A. et al., J. Reticuloendothel. Soc. 33, 263–273 (1983).

429. Characterization of the lysis of fresh human solid tumors by autologous lymphocytesactivated in vitro with phytohemagglutinin. Mazumder, A., Grimm, E.A. and Rosenberg, S.A.J. Immunol. 130, 958–964 (1983).

430. Analysis of natural killer effector and suppressor activity by intraepithelial lymphocytesfrom mouse small intestine. Mowat, A.McI., Tait, R.C., Mackenzie, S. et al., Clin. Exp.Immunol. 52, 191–198 (1983).

431. Enrichment of malignant cells from pleural effusions by Percoll density gradients.Nagasawa, T. and Nagasawa, S. Acta Cytol. 27, 119–123 (1983).

432. Suppression of human natural and antibody-dependent cytotoxicity by soluble factors fromunstimulated normal lymphocytes. Nair, M.P.N. and Schwartz, S.A. J. Immunol. 129,2511–2518 (1982).

433. Natural and antibody-dependent cell-mediated activity against Salmonella typhimurium byperipheral and intestinal lymphoid cells in mice. Nencioni, L., Villa, L., Boraschi, D. et al.,J. Immunol. 130, 903–907 (1983).

434. Analysis of human serological response to proteins of Chlamydia trachomatis. Newhall, W.J.,Batteiger, B. and Jones, R.B. Infect. Immun. 38, 1181–1189 (1982).

435. Monoclonal antibodies against human monocytes. II. Recognition of two distinct cellsurface molecules. Nunez, G., Ugolini, V., Capra, J.D. et al., Scand. J. Immunol. 16,515–523 (1982).

436. Isolation and identification of the naturally occurring, newborn spleen-associated suppressorcells. Peeler, K., Wigzell, H. and Peck, A.B. Scand. J. Immunol. 17, 443–453 (1983).

437. Isolation of chloroplasts for protein synthesis from spinach and Euglena gracilis by centrifugationin silica sols. Price, C.A. and Reardon, E.M. Methods Chloroplast Mol. Biol. 189–209 (1982).

438. A rapid simple method of basophil purification by density centrifugation on Percoll.Raghuprasad, P.K. J. Immunol. 129, 2128–2133 (1982).

439. Comparison of differentiating Dictyostelium discoideum cell types separated by an improvedmethod of density gradient centrifugation. Ratner, D. and Borth. W. Exp. Cell Res. 143,1–13 (1983).

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440. Separation and concentration of schizonts of Plasmodium falciparum by Percoll gradients.Rivadeneira, E.M., Wasserman, M. and Espinal. C.T. J. Protozool. 30, 367–370 (1983).

441. The Chediak-Higashi gene in humans. III. Studies on the mechanisms of NK impairment.Roder, J.C., Todd, R.F., Rubin, P. et al., Clin. Exp. Immunol. 51, 359–368 (1983).

442. Purification of mature schizonts of Plasmodium falciparum on colloidal silica gradients.Saul, A., Myler, P., Elliott, T. et al., Bulletin of the World Health Org. 60, 755–759 (1982).

443. Epidermal Langerhans cells as accessory cells in Con A stimulation of T lymphocytes in theguinea pig. Scheynius, A., Grönvik, K.-O. and Andersson, J. Scand. J. Immunol. 17,283–290 (1983).

444. Separation of different cell populations of rat liver by density gradient centrifugation in avertical rotor with self-generated Percoll gradients.Singh, B., Borrebaek, B. and Osmundsen, H.Acta Physiol. Scand. 117, 497–505 (1983).

445. Induction of degranulation and lysis of haemocytes in the freshwater crayfish. Astacusastacus by components of the prophenoloxidase activating system in vitro. Smith, V.J. andSöderhäll, K. Cell Tissue Res. 233, 295–303 (1983).

446. Separation of the haemocyte populations of Carcinus maenas and other marine decapods,and prophenoloxidase distribution. Söderhäll, K., Smith, V. Dev. Comp. Immunol. 7,229-239 (1983).

447. Plasmodium falciparum merozoites: isolation by density gradient centrifugation usingPercoll and antigenic analysis. Stanley, H.A., Langreth, S.G., Reese, R.T. et al., J. Parasitol.68, 1059–1067 (1982).

448. Gene expression during mammalian spermatogenesis.Stern, L., Kleene, K.C., Gold, B. et al.,Exp. Cell Res. 143, 247–255 (1983).

449. Delayed-type hypersensitivity induced in immunodeficient mice with syngeneic modified selfantigens: a suggestive model of autoimmune response. Tarcic, N. and Naor, D. Eur. J. Immunol.12, 961–966 (1982).

450. Interferon-induced autologous reactivity of human natural killer cells. Tarkkanen, J.Scand. J. Immunol. 17, 513–520 (1983).

451. Simultaneous preparation of merozoites and concentrated schizonts of Plasmodium falciparum.Southeast Asian J. Trop. Tharavanij, S. and Prasertsiriroj, V. Med. Pub. Hlth. 12, 518–523 (1981).

452. Regulation of the immune response in Plasmodium falciparum malaria. II. Antigen specificproliferative responses in vitro. Troye-Blomberg, M., Perlmann, H., Patarroyo, M.E. et al.,Clin. Exp. Immunol. 53, 345–353 (1983).

453. Functional relationship of human T lymphocytes, monocytes and interleukins. II Helperrequirements for phytohaemagglutinin-induced proliferation of human T lymphocytecolony-forming cells. Ulmer, A.J. and Flad, H.-D. Scand. J. Immunol. 18, 137–143 (1983).

454. Immunoregulatory activity of culture-induced suppressor macrophages. Veit, B.C. Cell. Immunol.72, 14–24 (1982).

455. Water- and solute-accessible spaces of purified peroxisomes. van Veldhoven, P., Debeer, L.J.and Mannaerts, G.P. Biochem. J. 210, 685–693 (1983).

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456. Inhibition of human natural killer activity by lysosomotropic agents. Verhoef, J. andSharma, S.D. J. Immunol. 131, 125–131 (1983).

457. Limiting dilution analysis of the frequency of human T cells and large granular lymphocytesproliferating in response to interleukin 2. I. The effect of lectin on the proliferative frequencyand cytotoxic activity of cultured lymphoid cells. Vose, B.M. and Bonnard, G.D.J. Immunol. 130, 687–693 (1983).

458. Limiting dilution analysis of the frequency of human T cells and large granular lymphocytesproliferating in response to interleukin 2. II. Regulatory role of interferon on proliferativeand cytotoxic precursors. Vose, B.M., Riccardi, C., Bonnard, D. et al., J. Immunol. 130,768–772 (1983).

459. 32Pi- and 45Ca-metabolism by matrix vesicle-enriched microsomes prepared from chickenepiphyseal cartilage by isosmotic Percoll density gradient fractionation. Warner, G.P.,Hubbard, H.L., Lloyd, G.C. et al., Calcif. Tissue Int. 35, 327–338 (1983).

460. An improved method of unit gravity sedimentation separation for murine peritonealmacrophage populations. de Weger, R.A., Verbakel, J.M.A., Oskam; R. et al., J. Immunol.Methods 61, 117–123 (1983).

461. Cell separation techniques. Wolowyk, M.W. In Red Cell Membranes - A MethodologicalApproach. (Ellory, J.C. and Young, J.D., Eds.) Academic Press 1–11 (1982).

462. Sodium-dependent cysteine transport in human red blood cells. Young, J.D., Wolowyk, M.W.,Jones, S.E.M. et al., Nature 279, 800–802 (1979).

463. Characterization of mast cell precursors by physical means: dissociation from T cells and T cellprecursors. Yung, Y.-P., Wang, S.-Y. and Moore, M.A.S. J. Immunol. 130, 2843–2848 (1983).

464. The handling of Listeria monocytogenes by macrophages: the search for an immunogenicmolecule in antigen presentation. Allen, P.M., Beller, D.I., Braun, J. et al., J. Immunol. 132,323–331 (1984).

465. Suppressive effect of human natural killer cells on pokeweed mitogen-induced B celldifferentiation. Arai, S., Yamamoto, H., Itoh, K. et al., J. Immunol. 131, 651–657 (1983).

466. The use of Percoll gradients for the preparation of subpopulations of human spermatozoa.Arcidiacono, A., Walt, H., Campana, A. et al., Int. J. Androl. 6, 433–445 (1983).

467. Entamoeba invadens and E. histolytica: Separation and purification of precysts and cysts bycentrifugation on discontinuous density gradients of Percoll. Avron, B., Bracha, R., Deutsch, M.R.et al., Exp. Parasit. 55, 265–269 (1983).

468. In vivo role of natural killer cells: involvement of large granular lymphocytes in the clearanceof tumor cells in anti-asialo GM1-treated rats. Barlozzari, T., Reynolds, C.W. andHerberman, R.B. J. Immunol. 131, 1024–1027 (1983).

469. Isolation of basolateral and brush-border membranes from rabbit kidney cortex. Vesicleintegrity and membrane sidedness of the basolateral fraction. Boumendil-Podevin, E.F. andPodevin, R.A. Biochem. Biophys. Acta 735, 86–94 (1983).

470. Erythrocyte age-fractionation using a Percoll-Renografin® density gradient: Application toauto-logous red cell antigen determinations in recently transfused patients. Branch, D.R.,Sy Siok Hian, A.L., Carlson, F. et al., Am. J. Clin. Path. 80, 453–458 (1983).

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471. Identification of three major synovial lining cell populations by monoclonal antibodiesdirected to Ia antigens and antigens associated with monocytes/macrophages and fibroblasts.Burmester, G.R., Dimitriu-Bona, A., Waters, S.J. et al., Scand. J. Immunol. 17, 69–82 (1983).

472. Polarization of NK cell cytoskeleton upon conjugation with sensitive target cells.Carpén, O., Virtanen, I., Lehto, V.-P. et al., J. Immunol. 131, 2695–2698 (1983).

473. Separation of bronchoalveolar cells from the guinea pig on continuous density gradients ofPercoll: Morphology and cytochemical properties of fractionated lung macrophages.Dauber, J.H., Holian, A., Rosemiller, M.E. et al., J. Reticulendothelial Soc. 33, 119–126 (1983).

474. Separate pools of cofactors for fatty acid activation and oxidation in peroxisomes.Debeer, L.J., Van Veldhoven, P. and Mannaerts, G.P. Ann. N.Y. Acad. Sci. 386, 401–403 (1982).

475. Human eosinophils and parasitic diseases. II. Characterization of two cell fractions isolatedat different densities. De Simone, C., Donelli, G., Meli, D. et al., Clin. Exp. Immunol. 48,249–255 (1982).

476. T cell subpopulations in CLL: Methods of T cell enrichment artificially alter proportions ofOKT4 and OKT8 positive cells. Dickinson, A.M., George, S. and Proctor, S.J. Clin. Exp.Immunol. 54, 525–531 (1983).

477. Lipid bodies: cytoplasmic organelles important to arachidonate metabolism in macrophages andmast cells. Dvorak, A.M., Dvorak, H.F., Peters, S.P. et al., J. Immunol. 131, 2965–2976 (1983).

478. Membranlipid-biosynthese in chloroplasten. (Article in German). Frentzen, M. and Heinz, E.Biologie in unserer Zeit 13, 178–187 (1983).

479. Established cell lines of mouse marrow adherent cells producing differentiation factor(s) forthe granulocyte-macrophage lineage. Godard, C.M., Augery, Y.L., Ginsbourg, M. et al.,In vitro 19, 897–902 (1983).

480. Pure population of viable neurons from rabbit dorsal root ganglia, using gradients of Percoll.Goldenberg, S.S.S. and DeBoni, U. J. Neurobiol. 14, 195–206 (1983).

481. Humoral immune response in experimental syphilis to polypeptides of Treponema pallidum.Hanff, P.A., Bishop, N.H., Miller, J.N. et al., J. Immunol. 131, 1973–1977 (1983).

482. Characterization and functional studies of the murine T lymphocyte response to Mycobacteriumleprae antigen. Haregewoin, A. and Louise, J. Scand. J. Immunol. 18, 225–233 (1983).

483. Inhibition of human natural killer (NK) activity and antibody dependent cellular cytotoxicity(ADCC) by lipomodulin, a phospholipase inhibitory protein.Hattori, T., Hiarta, F.,Hoffman, T. et al., J. Immunol. 131, 662–665 (1983).

484. Separation of bronchoalveolar cells from the guinea pig on continuous gradients of Percoll:Functional properties of fractionated lung macrophages. Holian, A., Dauber, J.H.,Diamond, M.S. et al., J. Reticuloendothelial Soc. 33, 157–164 (1983).

485. The morphological studies on characteristics of isolated parenchymal and non-parenchymalcells of the rat liver. Homma, S., Nagamori, S., Hasumura, S. et al., J. Electron Microsc. 31,389–396 (1982).

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486. High resolution comparison of Plasmodium knowlesi clones of different variant antigenphenotypes by two dimensional gel electrophoresis and computer analysis. Howard, R.J.,Aley, S.B. and Lemkin, P.F. Electrophoresis 4, 420–427 (1983).

487. Presence of an antigenic determinant common to rat IgE-potentiating factor, IgE-suppressivefactor, and Fc receptors on T and B lymphocytes. Huff, T.F., Yodoi, J., Uede, T. et al.,J. Immunol. 132, 406–412 (1984).

488. Surface labeling of thyrocytes isolated by a new method combining enzymatic digestion andPercoll gradient centrifugation. Islam, M.N., Briones-Urbina, R., Bako, G. et al., Anal.Biochem. 130, 260–265 (1983).

489. Monoclonal antibodies specific for Leishmania tropica. I. Characterization of antigensassociated with stage- and species-specific determinants. Jaffe, C.L. and McMahon-Pratt, D.J. Immunol. 131, 1987–1993 (1983).

490. Evidence for a nonoxidative mechanism of human natural killer (NK) cell cytotoxicity byusing mononuclear effector cells from healthy donors and from patients with chronicgranulomatous disease. Kay, H.D., Smith, D.L., Sullivan, G. et al., J. Immunol. 131,1784–1788 (1983).

491. Regulation of natural and antibody-dependent cellular cytotoxicity by staphylococcalenterotoxin A. Klimber, I., Bakacs, T. and Moore, M. Clin. Exp. Immunol. 54, 39–48 (1983).

492. Naturally cytotoxic tonsillar lymphocytes: A manifestation of heterogeneity among humanNK cells. Kimber, I. and Moore, M. Scand. J. Immunol. 17, 29–36 (1983).

493. Buoyant density constancy during the cell cycle of Escherichia coli. Kubitschek, H.E.,Baldwin, W.W. and Graetzer, R. J. Bacteriol. 155, 1027–1032 (1983).

494. Monoclonal anti-parasite and anti-RBC antibodies produced by stable EBV-transformed B celllines from malaria patients. Lundgren, K., Wahlgren, M., Troye-Blomgren, M. et al.,J. Immunol. 131, 2000–2003 (1983).

495. A routine source of human peritoneal macrophages. Maddox, Y., Foegh, M., Zeligs, B. et al.,Scand. J. Immunol. 19, 23–29 (1984).

496. Enhancement of susceptibility of HSV-1-infected cells to natural killer lysis by interferon.Munoz, A., Carrasco, L. and Fresno, M. J. Immunol. 131, 783–787 (1983).

497. Blockade of NK cells lysis is a property of monoclonal antibodies that bind to distinct regionsof T-200. Newman, W., Fast, L.D. and Rose, L.M. J. Immunol. 131, 1742–1747 (1983).

498. Isolation and characterization of the erythroid progenitor cell: CFU-E. Nijhof, W. andWierenga, P.K. J. Cell Biol. 96, 386–392 (1983).

499. Complement receptor distinguishes between two subsets of large granular lymphocytes withdifferent natural killer activity and cytochemical and ultrastructural features. Nocera, A.,Montesoro, E., Balbo, P. et al., Scand. J. Immunol. 18, 345–354 (1983).

500. Effect of ammonium chloride treatment on human polymorphonuclear leucocyte iodination.Philips, W.A., Hosking, C.S. and Shelton, M.J. J. Clin. Pathol. 36, 808–810 (1983).

501. Activation of helper T cells for B lymphocytes in primary mixed lymphocyte cultures.Pobor, G., Pettersson, S., Bandeira, A. et al., Scand. J. Immunol. 18, 207–215 (1983).

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502. Leu 7+ (HNK-1+) cells. II. Characterization of blood Leu 7+ cells with respect toimmunophenotype and cell density. Porwit-Ksiazek, A., Åman, P., Ksiazek, T. et al.,Scand. J. Immunol. 18, 495–499 (1983).

503. Antigen-specific proliferative human T lymphocyte clones with specificity for Chlamydiatrachomatis. Qvigstad, E., Digranes, S. and Thorsby, E. Scand. J. Immunol. 18, 291–297 (1983).

504. Class-II HLA restriction of antigen-specific human T lymphocyte clones. Qvigstad, E. andThorsby, E. Scand. J. Immunol. 18, 299–306 (1983).

505. Fractionation of untreated and inflammatory murine peritoneal macrophages on discontinuousPercoll density gradients. Rasmussen, S.-E, Rhodes, J.M. and Bennedsen, J. Acta Path.Microbiol. Immunol. Scand. Sect. C, 91, 299–304 (1983).

506. Two classes of bystander B cell response: activation requirements reflect those B cells in general.Ratcliffe, M.J.H. and Julius, M.H. J. Immunol. 131, 581–586 (1983).

507. The cytolytic T lymphocyte response to the murine cytomegalovirus I. Distinct maturationstages of cytolytic T lymphocytes constitute the cellular immune response during acuteinfection of mice with the murine cytomegalovirus. Reddehase, M.J., Keil, G.M. andKoszinowski, U.H. J. Immunol. 132, 482–489 (1984).

508. Separation of mouse lymphoblasts by discontinuous density centrifugation on Percoll gradients.Shields, J.M., Haston, W.S. and Wilkinson, P.C. J. Immunol. Methods 59, 159–165 (1983).

509. B cell maturation factor (BMF): a lymphokine or family of lymphokines promoting thematuration of B lymphocytes. Sidman, C.L., Paige, C.J. and Schreier, M.H. J. Immunol.132, 209–222 (1984).

510. Evidence that schistosome MHC antigens are not synthesized by the parasite but areacquired from the host as intact glycolproteins. Simpson, A.J.G., Singer, D., McCutchan, T.F.et al., J. Immunol. 131, 962–965 (1983).

511. Human granulocyte surface molecules identified by murine monoclonal antibodies.Skubitz, K.M., Pessano, S., Bottero, L. et al., J. Immunol. 131, 1882–1888 (1983).

512. Activation of antigen-enriched B cells. I. Purification and response to thymus-independentantigens. Snow, E.C., Vitetta, E.S. and Uhr, J.W. J. Immunol. 130, 607–613 (1983).

513. Activation of antigen-enriched B cells. II. Role of linked recognition in B cell proliferation tothymus-dependent antigens. Snow, E.C, Noelle, R.J., Uhr, J.W. et al., J. Immunol. 130,614–618 (1983).

514. Prostaglandin-mediated immunoregulation: reduced sensitivity of in vitro immunoglobulinproduction to indomethacin in rheumatoid arthritis. Staite, N.D., Ganczakowski, M.,Panayi, G.S. et al., Clin. Exp. Immunol. 52, 535–542 (1983).

515. Rapid, quantitative isolation of mitochondria from rat liver using Ficoll gradients in vertical rotors.Stocco, D.M. Anal. Biochem. 131, 453–457 (1983).

516. Natural killer (NK) cells as a responder to interleukin 2 (IL 2). I. Proliferative response andestablishment of cloned cells. Suzuki, R., Handa, K., Itoh, K. et al., J. Immunol. 130,981–987 (1983).

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517. An improved Percoll density gradient for measurements of experimental brain edema.Tengvar, C., Hultström, D. and Olsson, Y. Acta Neuropathol. 61, 201–206 (1983).

518. A micromethod for the quantitation of cellular proteins in Percoll with the Coomassiebrilliant blue dye-binding assay. Vincent, R. and Nadeau, D. Anal. Biochem. 135, 355–362 (1983).

519. Characterization of the humoral immune response in Plasmodium falciparum malaria. I.Estimation of antibodies to P. falciparum or human erythrocytes by means of microELISA.Wahlgren, M., Berzins, K., Perlmann, P. et al., Clin. Exp. Immunol. 53, 1771–1775 (1983).

520. Human bone marrow allograft recipients: production of, and responsiveness to, interleukin 2.Warren, H.S., Atkinson, K., Pembrey, R.G. et al., J. Immunol. 131, 1771–1775 (1983).

521. Schnelle dynamische H2O/Percoll-dichtegradienten fur mikropartikel in der analytischenulstrazentrifuge. Machtle, W. Colloid Polymer Sci. 262, 270–282 (1984).

522. Rapid preparation of synaptosomes from mammalian brain using nontoxic iso-osmoticgradient material (Percoll). Nagy, A. and Delgado-Escueta, A. J. Neurochem. 43,1114–1123 (1984).

523. Density separation of spleen cells increases fusion frequency and yield of Ig-producing hybridomas.Van Mourik, P., Rivero, R.A., Van der Kwast, Th.H. et al., J. Immunol. Metods 68, 45–53 (1984).

524. Isolation and purification of protoplasts from yeast like cells, hyphae and chlamydospores ofAureobasidium pullulans. White, C. and Gadd, G.M. J. Gen. Microbiol. 130, 1031–1034 (1984).

525. Abnormal pattern of erythrocyte aging in hereditary spherocytosis as shown by Percolldensity gradient centrifugation. Bell, C.M., Parker, A.C. and Maddy, A.H. Clin. Chim. Acta.142, 91–102 (1984).

526. Effect of spermatozoa selection on a simplified Percoll gradient in case of asthenozoospermia.Mathieu, C. et al., Andrologia 22, 467–471 (1990).

527. The use of discontinuous Percoll gradient to separate populations of cells from human bonemarrow and peripheral blood. Ellis, W.M., Georgious, G.M., Roberton, D.M. et al.,J. Immunol. Methods 66, 9–16 (1984).

528. Purification and spectral properties of Kalanchoe blossfeldiana leaf mitochondria.Rustin, P. and Alin, M.-F. Physiol. Veg. 22, 93–101 (1984).

529. Isolation and subfractionation of human peripheral blood mononuclear cells (PBMC) bydensity gradient centrifugation on Percoll. Ulmer, A.J., Scholz, W., Ernst, M., et al.,Immunobiol. 166, 238–250 (1984).

530. Selective inhibition of natural killer activity by the monoclonal antibodies OKT10 andVEP10 at the single cell level. Golightly, M.G., Koren, H.S., Travis, W.W. et al.,Cell. Immunol. 88, 382–392 (1984).

531. Staphylococcal protein A (SpA) does not induce production of interferon-gamma in humanmononuclear blood cells. Carlsson, R., Dohlstein, M., Boketoft, A. et al., Cell. Immunol.86, 136–144 (1984).

532. A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibitingCa++ transport and Ca++ ATPase activities. Epping, R.J. and Bygrave, F.L. Biochem. J. 223,733–745 (1984).

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533. Isolation of Kurloff cells by Percoll density gradient centrifugation. Protein labelling with35S-methionine of these cells. Landemore, G., Debout, C., Quillec, M. et al., Biol. Cell. 50,121–126 (1984).

534. Isoferritins in normal leucocytes. Jones, B.M., Worwood, M. and Jacobs, A. Br. J. Haematol.55, 73–81 (1983).

535. Mechanisms for eosinophil degranulation; release of the eosinophil cationic protein.Winqvist, I., Olofsson, T. and Olsson, I. Immunology 51, 1–8 (1984).

536. Lipid bodies; cytoplasmic organelles important to arachidonate metabolism in macrophages andmast cells. Dvorak, A.M., Dvorak, H.F., Peters, S.P. et al., J. Immunol. 131, 2965–2976 (1984).

537. Invasion of collagen gels by mouse lymphoid cells. Shields, J.M., Haston, W. and Wilkinson, P.C.Immunology 51, 259–268 (1984).

538. Epstein-Barr virus susceptibility of normal human B lymphocyte populations. Åman, P.,Ehlin-Henriksson, B. and Klein, G. J. Exp. Med. 159, 208–220 (1984).

539. Mechanism of cell-mediated cytotoxicity at the single cell level. Bradley, T.P., and Bonavida, B.Cell Immunol. 83, 199–207 (1984).

540. Separation of human T lymphocyte colony-forming cells on Percoll gradients. Tice, D.G.and Davey, F.R. Prep. Biochem. 13, 461–474 (1983).

541. Polarization of NK cell cytoskeleton upon conjugation with sensitive target cells.Carpen, O., Virtanen, I., Lehto, V.-P. et al., J. Immunol. 131, 2695–2698 (1983).

542. B cell maturation factor (BMF): a lymphokine or family of lymphokines promoting thematuration of B lymphocytes. Sidman, C.L., Paige, C.J. and Schreier, M.H. J. Immunol.132, 209–222 (1984).

543. Phytohemagglutinin-induced acquisition of T cell surface markers by rat bone marrow precursorcells in the absence of the thymic microenvironment. Pfeifer, R.W., Stillman, W.S. and Irons, R.D.Cell. Immunol. 82, 258–268 (1983).

544. Cell-mediated immunity in rats injected with an anti-malarial T cell line. Gross, A.,Frankenburg, S. and Londner, M.V. Cell. Immunol. 84, 14–21 (1984).

545. Interleukin-2 enhances natural killing of normal lymphocytes. Miyasaka, N., Darnell, B.,Baron, S. et al., Cell. Immunol. 84, 154–162 (1984).

546. Dichotomy in the tissue origin of schistosome acquired class I and class II major histocompatibilitycomplex antigens. Sher, A., Sacks, D.L., Simpson, A.J.G. et al., J. Exp. Med. 159, 952–957 (1984).

547. Lymphocyte transformation test in diagnosis of nickel allergy. Nordlind, K. Int. Archs.Allergy appl. Immunol. 73, 151–154 (1984).

548. Isolation of guinea pig basophils using anti-leukocyte antibody and density gradient centrifugationon Percoll. Graziano, F.M., Lipham, W., Swaminathan, N. et al., J. Immunol. Methods. 67,157–165 (1984).

549. Demonstration of transcriptional regulation of specific genes by phytochrome action.Silverthorne, J. and Tobin, E.M. Proc. Natl. Acad. Sci. USA 81, 1112–1116 (1984).

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550. Accessory cell function of human B cells. Scala, G., Kuang, Y.D., Hall, R.E. et al.,J. Exp. Med. 159, 1637–1662 (1984).

551. Identification of a strain-specific malarial antigen exposed on the surface of Plasmodiumfalciparum-infected erythrocytes. Leech, J.H., Barnwell, J.W., Miller, L.H. et al.,J. Exp. Med. 159, 1567–1575 (1984).

552. Target cells for avian myeloblastosis virus in embryonic yolk sac and relationship of celldifferentiation to cell transformation. Boettiger, D. and Durban, E. J. Virol. 49, 841–847 (1984).

553. Density gradient prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).II. Radioactive labelling of Percoll. Kjellen, L. and Pertoft, H. Anal. Biochem. 88, 283–284 (1978).

554. Assay for the determination of human carcinoma cells in circulating blood. Schwartz, R.,Walk, A. et al., J. Cancer Res. Clin. Oncol. 109, 122–129 (1985).

555. Adjustment of the osmolality of Percoll for the isopycnic separation of cells and cell organelles.Vincent, R. and Nadeau, D. Anal. Biochem. 141, 322–328 (1984).

556. Comparative studies on the lysosomal association of monomeric 239Pu and 241Am in ratand Chinese hamster liver: analysis with sucrose, metrizamide and Percoll density gradientsof subcellular binding as dependent on time. Sutterlin, U., Thies, W-G., Haffner, H. et al.,Radiation Res. 98, 293–306 (1984).

557. Irradiation induced cell death as related to cell cycle. Skog, S. and Tribukait, B.Acta Radiol. Oncol. 24, 87–94 (1985).

558. A rapid Percoll gradient procedure for the preparation of acetylcholine receptor-rich vesicles fromTorpedo marmorata electric organ. Lanz, O., Burgun, C. et al., Neurochem. Int. 7,331–340 (1985).

559. Preliminary investigation of the characteristics of fish lymphocytes separated on a Percolldiscontinuous gradient. Blaxhall, P.C. and Sheard, P.R. J. Fish Biol. 26, 209–216 (1985).

560. The cytotoxic reaction of hemocytes from the freshwater crayfish. Astacus astacus.Söderhäll, K., Wingren, A. et al., Cell. Immunol. 94, 326–332 (1985).

561. Subcellular fractionation of amoebapore and plasma membrane components of Entamoebahistolytica using self-generating Percoll gradients. Rosenberg, I. and Gitler, C.Mol. Biochem. Parasitol. 14, 231–248 (1985).

562. Association of decreased natural and antibody-dependent cellular cytotoxicity and productionof natural killer cytotoxic factor and interferon in neonates. Nair, M.P.N. et al.,Cell. Immunol. 94, 159–171 (1985).

563. Ontogenical studies on kinetics of lipopolysaccharide-induced response to bromelain-treatedmouse erythrocytes in mouse spleen cells. Kawaguchi, S. Cell. Immunol. 94, 60–72 (1985).

564. Nuclear mutation leads to an accelerated turnover of chloroplast-encoded 48 kd and 34,5 kdpolypeptides in thylakoids lacking photosystem II. Leto, K.L. et al., EMBO J. 4,1645–1653 (1985).

565. Endocytosis in Saccharomyces cerevisiae: internalization of enveloped viruses into spheroplasts.Makarow, M. EMBO J. 7, 1855–1860 (1985).

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566. Density gradient centrifugation (DGC) in Percoll for isolation and characterization ofOscillatoria spp. from Tjeukemeer. Bloem, J. and Moed, J.R. Freshwater Biol. 15,197–205 (1985).

567. Dissociation of stimulatory capacity in mixed lymphocyte reaction and proliferative responsesto pokeweed mitogen among peripheral blood non-T cells. Ko, H.S. and Ohene-Fianko, D.Immunol. Commun. 13, 563–569 (1984).

568. Morphological and functional characterization of interstitial cells from mouse testesfractionated on Percoll density gradients. Kerr, J.B. et al., Endocrinology 116, 1030–1043 (1985).

569. Preparation of cells from pig gastric mucosa. Isolation of parietal cells by isopycnic centrifugationon linear density gradients of Percoll. Mardh, S. et al., Acta Physiol. Scand. 122, 607–613 (1984).

570. Purification and properties of African swine fever virus. Carrascosa, A.L. et al., J. Virol. 54,337–344 (1985).

571. Percoll and Ficoll self-generated gradients by low-speed osmocentrifugation. Nunes, S.P. andGalembeck. F. Anal. Biochem. 146, 48-51 (1985).

572. Antigen presenting capacity of Percoll fractionated splenic B cells. Krieger, J. et al.,Fed. Proc. 44, 943 abstr. 3109 (1985).

573. Rapid method for isolation of normal human peripheral blood eosinophils on discontinuousPercoll gradients and comparison with neutrophils. Roberts, R. and Gallin, J.I. Blood 65,433–440 (1985).

574. Percoll density gradient separation of cells from human malignant effusions.Hamburger, A.W. et al., Br. J. Cancer 51, 253–258 (1985).

575. Use of a self generating Percoll gradient and single cell cytotoxicity assay to identify tumour-lyticproperties of inflammatory neutrophils.Lichtenstein, A. et al., J. Immunol. Methods 81,95–106 (1985).

576. Isolation of parietal cells from guinea pig gastric mucosa by a Percoll density gradient.Tani, N. et al., Tokai J. Exp. Clin. Med. 9, 395–401 (1984).

577. Role of brain lysosomes in the development of manganese toxicity in mice. Suzuki, H. et al.,Toxicol. Appl. Pharmacol. 71, 422–429 (1983).

578. Kinetics of tachyphylaxis to mediators of acute inflammation. Colditz, I.G. Immunol. 55,149–156 (1985).

579. Cyclophosphamide-induced suppressor cells in mice: suppression of the antibody responsein vitro and characterization of the effector cells. Segre, M. et al., Cell. Immunol. 91,443–454 (1985).

580. Leishmania tropica: differences in the antigenicity of promastigotes and amastigotes.Sadick, M.D. and Raff, H.V. Cell. Immunol. 91, 404–414 (1985).

581. Human erythrocytes adhering to schistosomula of Schistosoma mansoni lyse and fail to transfermembrane components to the parasite. Caulfield, J.P. and Ciani, C.M.L. J. Cell Biol. 101,158–166 (1985).

582. A method of isolating clean and viable zooxanthellae by density gradient centrifugation.Tytler, E.M. and Spencer Davies, P. Limnol. Oceanogr. 28, 1266–1268 (1983).

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583. The isolation by self forming gradients of Percoll plasma membrane enriched fractions frombovine retinal pigment epithelium. Otonello, S. and Maraini, G. Curr. Eye Res. 3,1085–1095 (1984).

584. Total sialic acid content of glycophorins during senescence of human red blood cells.Lutz, H.U. and Fehr, J. J. Biol. Chem. 254, 11177–11180 (1979).

585. Identification of osteoclast-specific monoclonal antibodies. Oursler, M.J., Bell, L.V. et al., J. Cell. Biol. 100, 1592–1600 (1985).

586. Differences in the volume distributions of human lung mast cell granules and lipid bodies:evidence that the size of these organelles is regulated by distinct mechanisms. Hammel, I.,Dvorak, A.m. et al., J. Cell Biol. 100, 1488–1492 (1985).

587. Preparation and fractionation of dispersed lung cells. (Article in Japanese). Urade, Y. andHayaishi, O. Toxicology Forum. 8, 209–216 (1985).

588. Subfractionation of human blood monocyte subsets with Percoll. Weiner, R.S. and Mason, R.R.Exp. Hematol. 12, 800–804 (1984).

589. Appearance of delayed-type hypersensitivity effector cells in murine gut mucosa.Shields, J.G. and Parrott, D.M.V. Immunol. 54, 771–776 (1985).

590. Isolation and characterization of syncytiotrophoblast plasma membrane human placenta.Khalfoun, D., Degenne, D. et al., FEBS LETT. 181, 33–38 (1985).

591. A comparison of the polypeptide composition of plasma membranes prepared from thewhite adipose tissue and adipocytes of the mouse, rat, rabbit, ox and chicken by a Percollself-forming gradient procedure. Tume, R.K., Lee, S.R. et al., Comp. Biochem. Physiol. 80B,127–134 (1985).

592. Ca2+, Mg2+-dependent endonuclease activity in different subpopulations of spleen cellsfrom normal and erythroleukemic mice. McMahon, G., Alsina, J. et al., Exp. Cell. Res. 157,462–474 (1985).

593. Purification of Treponema pallidum, Nichols strain, by Percoll density gradient centrifugation.Hanff, P.A., Norris, S.J. et al., Sex. Trans. Diseases 11, 275–286 (1984).

594. Specific leukotriene formation by purified human eosinophils and neutrophils.Verhagen, J., Bruynzeel, P.L.B. et al., FEBS LETT. 168, 23–28 (1984).

595. Human megakaryocytes. V. Changes in the phenotypic profile of differentiating megakaryocytes.Levene, R.B., Lamaziere, J.-M.D. et al., J. Exp. Med. 161, 457–474 (1985).

596. Rapid isolation of neuroblastoma membranes on Percoll gradients. Characterization andlipid composition. Chakravarthy, B.R., Spence, M.W. et al., Biochim. Biophys. Acta 812,223–233 (1985).

597. Processing and lysosomal localization of a glycoprotein whose secretion is transformationstimulated. Gal, S., Willingham, M.C. et al., J. Cell. Biol. 100, 535–544 (1985).

598. Neuronal regulation of astroglial morphology and proliferation in vitro. Hatten, M.E.J. Cell Biol. 100, 384–396 (1985).

599. Apparatus for Percoll microgravimetry determinations in experimental brain edema.Karlik, S.J. and Noseworthy, J.H. Stroke. 18, 661–664 (1987).

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600. High blood eosinophilia induced by oxygenation therapy. (English transl.).Regelsberger, H.S. Ehrfahrungs heilkunde 33, 766–770 (1984).

601. Vascular occlusion and disseminated intravascular coagulation in falciparum malaria.Mohanty, D., Marwaha, N. et al., Brit. Med. J. 290, 115–116 (1985).

602. Fractionation of human thymocytes and peripheral blood lymphocytes on Percoll densitygradients and DNA synthesis stimulation effect of mercuric chloride. Nordlind, K.Int. Arch. Allergy Appl. Immun. 75, 16–19 (1984).

603. The isolation of rat alveolar type II cells: a simplified approach using Percoll density centrifugation.Skillrud, D.M. and Martin, W.J. Lung 162, 245–252 (1984).

604. Subcellular localization and some properties of the enzymes hydrolysing inositolpolyphosphates in rat liver. Joseph, S.K. and Williams, R.J. FEBS LETT. 180, 150–154 (1985).

605. Effects of a cytosolic protein on the interaction of rat pancreatic zymogen granules in vitro.Rogers, J., Matthews, E.K. et al., Biochim. Biophys. Acta 897, 217–228 (1987).

606. Isolation of apical plasma membrane in rabbit gallbladder epithelium by Percoll densitygradient centrifugation. Botta, G., Meyer, G. et al., Biochim. Biophys, Acta 897, 315–323 (1987).

607. Preparation of cytoplasmic bodies (cytospheres) from isolated hepatocytes and their biochemicalproperties. Grivell, A.R., Berry, M.N. et al., Exp. Cell Res. 165, 11–28 (1987).

608. Monoclonal antibodies to LFA-1 and to CD4 inhibit the mixed leukocyte reaction after theantigen-dependent clustering of dendritic cells and T lymphocytes. Inaba, K. and Steinman, R.M.J. Exp. Med. 165, 1403–1417 (1987).

609. Dissociation of membrane binding and lytic activities of the lymphocyte pore-formingprotein (perforin). Young, J.D.-E., Damiano, A. et al., J. Exp. Med. 165, 1371–1382 (1987).

610. Rapid isolation of neuroblastoma plasma membranes on Percoll gradients. Characterizationand lipid composition. Chakravarthy, B.R., Spence, M.W. et al., Biochim. Biophys. Acta812, 223–233 (1985).

611. Platelets augment granulocyte aggregation and cytotoxicity: uncovering of their effects byimproved cell separation techniques using Percoll gradients. Boogaerts, M.A., Vercelotti, G.et al., Scand. J. Haematol. 37, 229–236 (1986).

612. Adhesion, spreading and fragmentation of human megakaryocytes exposed to subendothelialextracellular matrix: a scanning microscopy study. Caine, Y., Vlodavsky, I. et al.,Scanning Electron Microsopy 3, 1087–1094 (1986).

613. Isolation of spinach leaf peroxisomes and mitochondria by discontinuous Percoll densitygradient centrifugation. Liang, Z., Zon, Y. et al., Kexue Tongbao 31, 847–851 (1986).

614. Relationship between cell age, glutathione and cation concentrations in sheep erythrocyteswith a normal and a defective transport system for amino acids. Fisher, T.J., Tucker, E.M. et al.,Biochim. Biophys. Acta 884, 211–214 (1986).

615. Adenosine triphosphate content of Mycobacterium leprae isolated from armadillo tissue byPercoll buoyant density centrifugation. Kvach, J.T., Neubert, T.A. et al., Int. J. Leprosy 54,1–10 (1986).

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616. Renin granules isolated from rat kidney cortex by continuous silica (Percoll) density gradientcentrifugation. Matsumura, Y., Takeshita, M. et al., Renal Physiology 9, 241–248 (1986).

617. Single step purification procedure for the rapid separation of equine leucocytes.Sedgwick, A.D., Morris, T. et al., Vet. Res. Commun. 10, 445–452 (1986).

618. The effect of chloroquine on equilibrium density in a Percoll gradient of liver lysosomalpopulations. Colombo, M.I. and Bertini, F. Microsc. Electron. Biol. Cel. 9, 205–211 (1985).

619. Localization of pancreatic enzyme secretion-stimulation activity in zymogen granule of therat pancreas. Fukuoka, S.-I., Hawajiri, H. et al., Biochim. Biophys. Acta 884, 18–24 (1986).

620. Measurement of freshwater micro-algal cell density with Percoll density gradients.Lavoie, A., Mouget, J.-L. et al., J. Microbiol. Methods 4, 251–259 (1986).

621. Isolation of highly purified and more native nuclei of Physarum polycephalum utilizingSurfynol, hexylene glycol and Percoll. Nothacker, K.-D. and Hildebrandt, A. Eur. J. Cell Biol.39, 278–282 (1985).

622. The separation of insect haemocyte types on Percoll gradients; methodology and problems.Mead, G.P., Ratcliffe, N.A. et al., J. Insect Physiol. 32, 167–177 (1986).

623. Concentration of Plasmodium ovale and Plasmodium vivax-infected erythrocytes from non-humanprimate blood using Percoll gradients.Andrysiak, P.M., Collins, W.E. et al., Am. J. Trop.Med. Hyg. 35, 251–254 (1986).

624. Cytochemical enzyme staining of fish lymphocytes separated on a Percoll gradient.Blaxhall, P.C. and Hood, K. J. Fish. Biol. 27, 749–755 (1985).

625. Separation of hexose-transporting from non-transporting LLC-PK1 cells on density gradients.Weiss, E.R. and Cook, J.S. Am. J. Physiol. 250, 199–206 (1986).

626. Separation of human megakaryocytes by state of differentiation on continuous gradients ofPercoll: size and ploidy analysis of cells identified by monoclonal antibody to glycoprotein IIbIIIa.Ishibashi, T., Ruggeri, Z.M. et al., Blood 67, 1286–1292 (1986).

627. Biochemical characterization of plasma membranes and intracellular membranes isolatedfrom human platelets using Percoll gradients. Fauvel, J., Chap, H. et al., Biochem. Biophys. Acta856, 155–164 (1986).

628. Percoll-purified Treponema pallidum, an improved fluorescent treponemal antibody-adsorbedantigen. Hanff, P.A., Fernandez, C. et al., J. Clin. Microbiol. 23, 980–982 (1986).

629. Cell fractions from rat rib growth cartilage. Pawlowski, A., Makower, A.-M. et al.,Exp. Cell Res. 164, 211–222 (1986).

630. Steroid metabolism by cells from human chorion laeve isolated on Percoll gradients.Gibb, W. and Lavoie, J.-C. Can. J. Physiol. Pharmacol. 64, 213–217 (1986).

631. Renin granules isolated from rat kidney cortex by continuous colloidal silica (Percoll)density gradient centrifugation.Matsumura, Y., Takeshita, M. et al., Renal Physiol. 9,241–248 (1986).

632. Separation of bacteria from a methanogenic waste-water population by utilizing a self-generatingPercoll gradient. Scherer, P. J. Appl. Bacteriol. 55, 481–486 (1983).

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633. A rapid isolation procedure of plasma membranes from human neutrophils using self-generating Percoll gradients. Importance of pH in avoiding contamination by intracellularmembranes. Record, M., Laharrague, P. et al., Biochem. Biophys. Acta 819, 1–9 (1985).

634. Preparation of rat gastric heavy and light microsomal membranes enriched in (H+-K+)-ATPaseusing 2H2O and Percoll gradients. Wha, B.I., Davis, J.P. et al., Biochem. Biophys. Res.Commun. 131, 905–911 (1985).

635. Optimized density gradient separation of leukocyte fractions from whole blood by adjustmentof osmolarity. Braide, M. and Bjursten, L.M. J. Immunol. Methods 93, 183–191 (1986).

636. Effect of human recombinant interleukin 2 on natural killing of low density Percoll fraction cells.Lange, A., Flad, H.-D. et al., Immunol. Lett. 12, 243–250 (1986).

637. Purification of dispersed rat adrenal zona glomerulosa cells by Percoll density gradientcentrifugation and the isolation of a population of cells highly responsive to adrenocorticotrophin.Chu, F.W. and Hyatt, P.J. J. Endocr. 109, 351–358 (1986).

638. Is cytochrome b558 translocated into the plasma membrane from granules during theactivation of neutrophils? Yamaguchi, T., Kaneda, M. et al., J.Biochem. 99, 953–959 (1986).

639. The role of superoxide anion and lysosomal enzymes in anti-listerial activity of elicitedperitoneal macrophages. Hashimoto, S., Nomoto, K. et al., Scand. J. Immunol. 24,429–436 (1986).

640. Rapid separation of rat peritoneal mast cells with Percoll. Nemeth, A. and Roehlich, P.Eur. J. Cell Biol. 20, 272–275 (1980).

641. A rapid method for isolation of synaptosomes on Percoll gradients. Dunkley, P.R., Jarvie. P.E.et al., Brain Res. 372, 115–129 (1986).

642. A survey of buoyant density of microorganisms in pure cultures and natural samples.Guerrero, R., Pedros-Alio, C. et al., Microbiologia 1, 53–65 (1985).

643. Preparation of nasopharyngeal secretions for immunofluorescence by one-step centrifugationthrough Percoll. Ukkonen, P. and Julkunen, I. J. Virol. Methods 15, 291–301 (1987).

644. On the use of Percoll as a marker for liposomes in electron microscopy of mouse spleen tissue.Cudd, A. and Nicolau, C. J. Microencapsulation 3, 275–282 (1986).

645. Mathematical model for determining the effect of intra-cytoplasmic inclusions on volumeand density of microorganisms. Mas, J., Pedros-Alio, C. et al., J. Bacteriol. 164, 749–756 (1985).

646. Buoyant density changed due to intracellular content of sulfur in Chromatium warmingiiand Chromatium vinosum. Guerrero, R., Mas, J. et al., Arch. Microbiol. 137, 350–356 (1984).

647. The influence of poly-beta-hydroxybutyrate accumulation on cell volume and buoyant densityin Alcaligenes eutrophus. Pedros-Alio, C., Mas, J. et al., Arch. Microbiol. 143, 178–184 (1985).

648. Separation of human X- and Y-bearing sperm using Percoll density gradient centrifugation.Kaneko, S., Yamaguchi, J. et al., Fertility and Sterility 40, 661–665 (1983).

649. Morphological and functional characteristics of rat Leydig cells isolated on Percoll gradients:is Leydig cell heterogeneity in vitro an artifact? Lasw, A.O., Wreford, N.G.M. et al.,Mol. Cell. Endocr. 42, 73–90 (1985).

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650. Preparation of pure hepatocytes and reticuloendothelial cells in high yield from a single ratliver by means of Percoll centrifugation and selective adherence. Smedsrod, B. and Pertoft, H.J. Leukocyte. Biol. 38, 213–230 (1985).

651. Transport of NAD+ in Percoll-purified potato tuber mitochondria. Neuburger, D., Day, D.A.et al., Plant Physiol 78, 405–410 (1985).

652. Estrogen and progestin receptors in mouse vaginal epithelium and fibromuscular wall.Uchima, F.-D.A,. Edery, M. et al., Biochim, Biophys. Acta 841, 135–138 (1985).

653. Isolation of proteolipid from subpopulations of membranes from a calcifiable bacterium.Dereszewski, G., Tingle, J. et al., J. Dent. Res. 63, 242 (abstract 644) (1984).

654. Intactness and uptake properties of leaf peroxisomes purified in Percoll using different solutes:sedimentation behavior. Betsche, T. and Mueller, G. Plant Physiol. 77, 88 (abstract 481) (1985).

655. The binding of con A and other lectins to surface glycoproteins: A comparison of a subcutaneoustumour and its liver metastases. Chan, W-S., Jackson, A. et al., Invasion Metastasis 5,233–244 (1985).

656. Trophic influences of luteinizing hormone on steroidogenesis by Percoll-separated rat Leydigcells in culture. Bord, M.J., Shaper, J.H. et al., Ann. N.Y. Acad. Sci. 438, 329–345 (1984).

657. Purification of non-synaptic and synaptic mitochondria and plasma membranes from ratbrain by a rapid Percoll gradient procedure. Rendon, A. and Masmoudi, A.J. Neurosci. Methods 14, 41–51 (1985).

658. Morphological and functional characterization of interstitial cells from mouse testesfractionated on Percoll density gradients. Kerr, J.B., Robertson, D.M. et al., Endocrinology116, 1030–1043 (1985).

659. Degree of chromatin fragmentation and frequency of nuclear pyknosis in Percoll- fractionatedthymocytes of irradiated rats. Borisova, E.A., Chukhlovin, A.B. et al., Int. J. Radiat. Biol.51, 421–428 (1987).

660. Purification of human sperm by a discontinuous Percoll density gradient with an inner column.Kaneko, S., Oshio, S. et al., Biol. Reprod. 35, 1059–1063 (1986).

661. Marrow function reconstitution by fraction 3 of Percoll density gradient separated cells.Ekert, H., Ellis, W.M. et al., Transplantation 42, 58–60 (1986).

662. Increase in progressive motility and improved morphology of human spermatozoa followingtheir migration through Percoll gradients. Pousette, A., Akerlof, E. et al., Int. J. Androl. 9,1–13 (1986).

663. Minicell isolation by isopycnic sedimentation in gradients of silica: an updated protocol.Bellemare, G., Estable, M.C. et al., Analects 14, 1–5 (1986).

664. Separation of CFU-GM from normal human bone marrow mononuclear cells on densitygradients of polyvinyl pyrrolidone coated silica gel (Percoll).Kothari, V.C., Advani, S. et al.,Haematologica 70, 477–481 (1985).

665. Differential expression of lymphokine-activated killer cells and natural killer cells in adoptivetransfer experiments utilizing fractionated bone marrow. Merluzzi, V.J., Trail, P.A. et al.,J. Immunol. 137, 2425–2427 (1986).

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666. CFU-c enrichment from human bone marrow using a discontinuous Percoll gradient andsoybean agglutinin in comparison with Ficoll-Paque.Georgiou, G.M., Roberton, D.M. et al.,Clin. Exp. Immunol. 53, 491–496 (1983).

667. Purification of haemopoietic progenitor cells from patients with chronic granulocyticleukaemia using Percoll density and elutriation. Martin, H., Hibbin, J.A. et al., Br. J. Haematol.63, 187–198 (1986).

668. Resistance of cytotoxic T lymphocytes to the lytic effects of their toxic granules. Verret, C.R.,Firmenich, A.A. et al., J. Exp. Med. 166, 1536–1547 (1987).

669. A technique for separating high- and low-quality embryos of the scallop, Pecten maximus L.Devauchelle, N., Dorange, G. and Fuare, C. Aqua-culture. 120, 341–346 (1994).

670. A strategy for an improved separation of mammalian spermatids. Loir, M. and Lanneau, M.Gamete Res. 6, 179–188 (1982).

671. Size and density characterization of human committed and multipotent hematopoietic progenitors.Lasky, L.C. and Zanjani, E.D. Exp. Hematol. 13, 680–684 (1985).

672. Isolation of malignant cells from human bone marrow using a discontinuous Percoll gradient.Macfarlane, S.D., Tauro, G.P. et al., Am. J. Hematol. 22, 403–407 (1986).

673. Positive correlation between the level of protein-carboxyl methylase in spermatozoa andsperm motility. Gagon, C., de Lamirande, E. et al., Fertility Sterility 45, 847–853 (1986).

674. Interstitial cell heterogeneity in rat testes. Bhalla, V.K., Flasch, M.V. et al., J. Biol. Chem.262, 5322–5332 (1987).

675. The use of discontinuous Percoll gradients to separate populations of cells from humanbone marrow and peripheral blood. Ellis, W.M., Georgiou, G.M. et al., J. Immunol. Methods66, 9–16 (1984).

676. Scanning electron microscope study of platelet release by canine megakaryocytes in vitro.Handagama, P.J., Jain, N.C. et al., Am. J. Vet. Res. 48, 1003–1006 (1987).

677. Improved methods for the isolation of individual and clustered mitotic chromosomes.Gasser, S.M. and Laemmli, U.K. Exp. Cell Res. 173, 85–98 (1987).

678. Circulating proplatelets: Isolation and quantitation in healthy rats and in rats with inducedacute blood loss. Handagama, P.J., Feldman, B.F. et al., Am. J. Vet. Res. 48, 962–965 (1987).

679. Selective isolation of human X-bearing sperm by differential velocity sedimentation inPercoll density gradients. Kaneko, S., Oshio, S. et al., Biomed. Res. 5, 187–194 (1984).

680. Skeletal muscle cell populations. Yablonka-Reuveni, Z. and Nameroff, M. Histochem. 87,27–38 (1987).

681. Percoll separation of leukemic leukocytes for MLC matching prior to bone marrowtransplantation. Hakos, G., Rayment, C. et al., Transplantation. 39, 323–326 (1985).

682. Human leukocyte interferon inhibits human chorionic gonadotropin stimulated testosteroneproduction by porcine Leydig cells in culture. Orava, M., Cantell, K. et al., Biochem.Biophys. Res. Commun. 127, 809–815 (1985).

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683. Isolation of apical plasma membrane in rabbit gallbladder epithelium by Percoll density gradientcentrifugation. Botta, G., Meyer, G. et al., Biochimica et Biophysica Acta. 897, 315–323 (1987).

684. Problems in separation of small numbers of platelets from unbound ligands. George, J.N.Thrombosis Res. 44, 561–564 (1986).

685. Isolation and characterization of parasites and host ghosts from erythrocytes infected withPlasmodium chabaudi. Wunderlich, F., Helwig, M. et al., Mol. Biochem. Parasitol. 23,103–115 (1987).

686. Use of Percoll gradients and centrifugal elutriation for separation of oyster haemocytes.Bachare, E. et al., Dev. Comp. Immunol. 10, 639–640 (1986).

687. Characterization of granzymes A and B isolated from granules of cloned human cytotoxic Tlymphocytes. Krähenbühl, O., Rey, C. et al., J. Immunology 141, 3471–3477 (1988).

688. Separation of Syntrophomonas wolfei from Methanospirillum hungatii in syntrophic co-culturesby using Percoll gradients. Beaty, P.S. et al., Appl. Environ. Microbiol. 53, 1183–1185 (1987).

689. Inclusion bodies in xenosomes purified on Percoll gradients as revealed by electron microscopy.Soldo, A.T. et al., J Protozool. 34, 6–9 (1987).

690. Degree of chromatin fragmentation and frequency of nuclear pyknosis in Percoll-fractionatedthymocytes of irradiated rats. Borisova, E.A. et al., Int. J. Radiat. Biol. Relat. Stud. Phys.Chem. Med. 5, 421–428 (1987).

691. Isolation by Percoll gradient centrifugation and radiolabeling of bloodstream forms ofTrypanosoma cruzi. Rimoldi, M.T. et al., Revista Argentina de Microbiologia. 18, 41–44 (1986).

692. Fractionation of Plasmodium chabaudi-infected erythrocytes into parasites and ghosts.Wunderlich, F. et al., Z. Parasitenkd. 71, 545–551 (1985).

693. Pathogenesis of arteritis of SL/Ni mice. Possible lytic effect of anti-gp70 antibodies onvascular smooth muscle cells. Miyazawa, M. et al., J. Exp. Med. 166, 890–908 (1987).

694. Isolation, characterization, and in vitro cultivation of keratinocyte subfractions from adultNMRI mouse epidermis: epidermal target cells for phorbol esters. Gross, M. et al.,Experimental Cell Research. 171, 460–474 (1987).

695. The isolation by self forming gradients of Percoll of plasma membrane enriched fractionsfrom bovine retinal pigment epithelium. Ottonello, S. et al., Curr. Eye Res. 3, 1085–1096 (1984).

696. Separation of Cryptosporidium species oocysts from feces by using a Percoll discontinuousdensity gradient. Waldman, E. et al., J. Clin. Microbiol. 23, 199–200 (1982).

697. Separation of cells and other particles by centrifugation in colloidal silica solutions. Pertoft, H.et al., Phys. Colloids Macromol. Proc. Inf. Symp. (Ranby, Ed.) Blackwell 75–80 (1987).

698. Selective isolation of mature hamster sperm using Percoll density gradient centrifugation.Oshio, S. et al., Zoological Science (Tokyo) 1, 940 (1986).

699. Percoll fractionation of normal human and macular corneal dystrophy keratocytes: subcellularorganelle analysis and comparision. Wyszynski, R.E. et al., Invest. Opthalmol. Visual. Sci.27, 65 (1986).

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700. Fraccionamiento de células hemopoyéticas medulares y circulantes humanas en gradientediscontinuo de Percoll. Determinación de CFU GM de cada fracción (Article in Spanish).Bianchi de di Risio, C. et al., Acta Bioquimica Clinica Latinoamericana 21, 11–20 (1987).

701. Erythrocyte density separation on discontinuous Percoll gradients. Mackie, L.H. et al.,Biotechnology. 24, 227–230 (1987).

702. Resolution of multiple endosomal compartments associated with the internalization of epidermalgrowth factor and transferrin. Gorman, R.M. et al., J. Cell. Physiol. 131, 158–166 (1987).

703. Isolation of monkey aortic lysosomes using Percoll density gradient. Dhaunsi, G.S. et al.,Biochem. Int. 16, 787–796 (1987).

704. Polyclonal stimulation of resting B lymphocytes by antigen-specific T lymphocytes.DeFranco, A.L. et al., J. Exp. Med. 159, 861–880 (1984).

705. Isolation of Cryptosporidium oocysts and sporozoites using dicontinuous sucrose andisopycnic Percoll gradients. Arrowood, M.J. et al., J. Parasitol, 73, 314–319 (1987).

706. Rapid, single-step isolation of equine neutrophils on a discontinuous Percoll density gradient.Pycock, J.F. et al., Res. Vet. Sci. 42, 411–412 (1987).

707. Linear Percoll gradient centrifugation of rat anterior pituitary cells. A simple method forprolactin cell enrichment. Velkeniers, B. et al., J. Endocrinol. Invest. 10, 247–253 (1987).

708. O2 consumption and superoxide dismutase content in purified mitochondria from soybeanroot nodules. Puppo, A. et al., Plant Science. 50, 3–12 (1987).

709. Isolation of highly purified rat liver lysosomal membranes using two Percoll gradients.Symons, L.J. et al., Anal. Biochem. 164, 382–390 (1987).

710. The preparative isolation of mitochondria from Chinese hamster ovary cells.Madden, E.A. et al., Anal. Biochem. 163, 350–357 (1987).

711. A methodological study of discontinuous Percoll-gradient separation of pancreatic islet from rats.Vonen, B. et al., Scand. J. Clin. Lab. Invest. 47, 415–420 (1987).

712. Isolation of murine pluripotent hemopoietic stem cells in the G0 phase. Miyama-Inaba, M.et al., Biochem. Biophys. Res. Commun. 147, 687–694 (1987).

713. Use of Percoll gradients for isolation of human placenta mitochondria suitable for investigatingouter membrane proteins. Gasnier, F. et al., Anal. Biochem. 212, 173–178 (1993).

714. In vitro expression of chloroplast genes in lysates of higher plant chloroplasts. Bard, J. et al.,Proc. Natl. Acad. Sci. 82, 3983–3987 (1985).

715. Separation of newborn rat epidermal cells on discontinuous isokinetic gradients of Percoll.Brysk, M. et al., J. Invest. Dermatol. 77, 205–209 (1981).

716. Characteristics of mouse thymocyte-derived, interleukin 2-activated killer cells and theirprecursors. Yagi, H. et al., Japanese. J. Cancer. Res. (Gann). 78, 721–728 (1987).

717. Development of the phagaocytic and cidal capacity during maturation of myeloid cells:studies on cells from patients with chronic myelogenous leukaemia. Segel, E.K. et al.,British J. Haematol. 67, 3–10 (1987).

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718. Specific antibody responses by high and low-density human peripheral blood B cells: T-helpercells and T-cell replacing factor (TFR) act on different B-cell subpopulations. Callard, R.E.et al., Immunology. 62, 451–456 (1987).

719. A rapid Percoll technique for the purification of human basophils. Warner, J.A. et al.,J. Immunol. Methods. 105, 107–110 (1987).

720. Subpopulations of MCF7 cells separated by Percoll gradient centrifugation: A model toanalyze the heterogeneity breast cancer. Resnicoff, M. et al., Proc. Natl. Acad. Sci. USA. 84,7295–7299 (1987).

721. Immunocytochemical localization of glucocorticoid receptors in cells, cytoplast, and nucleoplasts.LaFond, R.E. et al., Exp. Cell. Research. 175, 52–62 (1988).

722. Distribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes insucrose and Percoll gradients. Gratzl, M. Analytical Biocheme. 142, 148–154 (1984).

723. Separation of human endothelial cells from fibroblasts by centrifugation in Percoll gradients.Sbarbati, R. Bioscience Reports 5, 469–472 (1985).

724. Isolation of mitochondria from soybean leaves on discontinuous Percoll gradients.Hrubec, T.C. et al., Plant. Physiol. 77, 1010–1012 (1985).

725. Distribution of decay-accelerating factor in the peripheral blood of normal individuals andpatients with paroxysmal nocturnal hemoglobinuria. Kinoshita, T. et al., J. Exp. Med. 162,75–92 (1985).

726. Low-temperature culture of human islets isolated by the distention method and purifiedwith Ficoll or Percoll gradients. Scharp, D.W. et al., Surgery 102, 869–879 (1987).

727. Biochemical and ultrastructural effects of monensin on the processing, intracellular transport,and packaging of myeloperoxidase into low and high density compartments of humanleukemia (HL-60) cells. Akin, D.T. et al., Arch. Biochem. Biophys. 257, 451–463 (1987).

728. Very long chain fatty acid b-oxidation by subcellular fractions of normal and Zellwegersyndrome skin fibroblasts. Singh, H. et al., Arch. Biochem. Biophys. 257, 302–314 (1987).

729. Separation of human epidermal Langerhans cells by density gradient centrifugation on acolloidal silica (Percoll) gradient method and autologous, allogeneic mixed skin cell leukocyteculture reactions. (Article in Korean). Bae, Y.J. et al., Korean J. Dermatol. 25, 299–308 (1987).

730. Separation of bovine erythrocytes infected with Theileria sergenti by "Percoll-Conray"density gradient. Yagi, Y. et al., Jpn. J. Vet. Sci. 49, 745–750 (1987).

731. Application of Percoll density gradients in studies of the adhesion of Streptococcus pyogenesto human epithelial cells. Tylewska, S.K. et al., Curr. Microbiol. 16, 129–136 (1987).

732. The essential role of B cell stimulatory factor 3 (BSF-2/IL-6) for the terminal differentiationof B cells. Muraguchi, A. et al., J. Exp. Med. 167, 332–344 (1988).

733. Variation in plants regenerated from vacuolate and evacuolate protoplasts. Lawrence, W.A.et al., Plant. Science. 50, 125–132 (1987).

734. MCH class II antigen-bearing dendritic cells in pulmonary tissues of the rat. Regulation ofantigen presentation activity by endogenous macrophage populations. Holt, P.G. et al.,J. Exp. Med. 167, 262–274 (1988).

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735. Subcellular localization of the common shikimate pathway enzymes in Pisum sativum L.Mousdale, D.M et al., Planta 163, 241–249 (1985).

736. In vivo obtained antigen presented by germinal center B cells to T cells in vitro. Kosco, M.H.et al., J. Immunol. 140, 354–360 (1988).

737. Enrichment of human marrow T-cell precursors by complement-dependent cytotoxicity withmonoclonal antibodies and Percoll gradient centrifugation.Ahmad, M. et al., Exp. Hematol.15, 1035–1060 (1987).

738. Isolation of high molecular weight plant nuclear DNA suitable for use in recombinant DNAtechnology. Kiss, T. et al., Acta. Biochim. Biophys. Acad. Sci. Hung. 22, 1–5 (1987).

739. Cytochrome b-560-deficient mitochondria from Daucus carota roots. Vaz-Pinto, V. et al.,Plant Physiol. Biochem. 25, 415–422 (1987).

740. Fractionation of mouse malarious blood according to parasite developmental stage, usingPercoll-sorbitol gradient. Ginsburg, H. et al., Ann. Parasitol. Hum. Comp. 62, 418–425 (1987).

741. The mechanism of iron uptake by primary cultured rat hepatocytes. (Article in Japanese).Tsuyuki, H. J. Nippon. Med. Sch. 54, 551–562 (1987).

742. Myogenicity in vitro and in vivo of mouse muscle cells separated on discontinuous Percollgradients J. Morgan, J.E. J. Neurol. Sci. 85, 197–207 (1988).

743. The heterogeneity of isolated adult rat Leydig cells separated on Percoll density gradients:an immunological, cytochemical, and functional analysis.Hedger, M.P. et al., Endocrinology121, 1824–1838 (1987).

744. Sexing of human sperm by discontinuous Percoll density gradient and its clinical application.Iizuka, R. et al., Human Reproduction 2, 573–575 (1987).

745. The use of Percoll gradients for the preparation of subpopulations of human spermatozoa.Arcidiacono, A. et al., Int. J. Andrology 6, 433–445 (1983).

746. Properties of luminal and contraluminal vesicles isolated from rat kidney cortex by Percollgradients. Leal-Pinto, E. et al., Acta. Cient. Venez. 38, 157–163 (1987).

747. Comparative study of eosinophil purification on nycondenz, metrizamide and Percolldensity gradients. Brattig, N.W. et al., Eur. J. Haematol. 39, 148–153 (1987).

748. Enrichment and selection of hybrid hybridomas by Percoll density gradient centrifugationand flourescent-activated cell sorting. Koolwijk, P. et al., Hybridoma 7, 217–225 (1988).

749. T cell-dependent hapten-specific and polyclonal B cell responses require release ofinterleukin 5. Rasmussen, R. et al., J. Immunology 140, 705–712 (1988).

750. Mechanism of spontaneous activation of B cells in patients with systemic lupus erythematosus.Tanaka, Y. et al., J. Immunology 140, 761–767 (1988).

751. Characterization of Caedibacter endonucleobionts from the macronucleus of Parameciumcaudatum and the identification of a mutant with blocked R-body synthesis. Schmidt, H.J.et al., Exp. Cell. Research 174, 49–57 (1988).

752. The purification of sporocysts and sporozoites from Eimeria tanella oocysts using Percolldensity gradients. Dulski, P. and Turner, M. Avian Diseases 32, 235–239 (1988).

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753. A rapid Percoll gradient procedure for isolation of synaptosomes directly from an S1 fraction:viability of subcellular fractions. Harrison, S.M. et al., Brain. Res. 441, 72–80 (1988).

754. A rapid Percoll gradient procedure for isolation of synaptosomes directly from an S1 fraction:homogeneity and morphology of subcellular fracions. Dunkley, P.R. et al., Brain. Res. 441,59–71 (1988).

755. Purification of plant mitochondria on silica sol gradients. Moore, A.L. et al., Methods Enzymol.148, 415–420 (1987).

756. Subcellular localization of a variable surface glycoprotein phosphatidylinositol-specificphospholipase-C in African trypanosomes. Grab, D.J. et al., J. Cell. Biol. 105, 737–746 (1987).

757. Differential expression of fibronectin in a rat histiocytoma: possible role of fibronectin intumor cell adhesion. Pande, G. et al., Exp. Cell. Research 174, 41–48 (1988).

758. Fractionation of subcellular membrane organelles of the yeast Candida maltosa in Percollgradients. (Article in Russian). Sharyshev, A.A. et al., Biologicheskie Membrany (BiologicalMembranes) 5, 187–197 (1988).

759. Enrichment of heterokaryocytes between mesophyll and epidermis protoplasts by densitygradient centrifugation after electric fusion. Kamata, Y. and Nagata, T. Theor. Appl. Genet.75, 26–29 (1987).

760. Total sialic acid content of glycophorins during senescence of human red blood cells.Lutz, H.U. and Fehr, J. J. Biol. Chem. 254, 11177–11180 (1979).

761. Naturally occurring autoantibodies to exoplasmic and cryptic regions of band 3 protein, themajor integral membrane protein of human red blood cells.Lutz, H.U. et al., J. Immunol.133, 2610–2618 (1984).

762. Naturally occurring anti-band-3 antibodies and complement together mediate phagocytosisof oxidatively stressed human erythrocytes. Lutz, H.U. et al., Proc. Natl. Scad. Sci. USA 84,7368–7372 (1987).

763. Re-evaluation of the structural integrity of red-cell glycoproteins during aging in vivonutrient deprivation. Brovelli, A. et al., Biochem. J. 242, 115–121 (1987).

764. Red cell clearance (a review). Lutz, H.U. Biomed. Biochim. Acta. 46, 65–71 (1987).

765. Red cell density and red cell age. Lutz, H.U. Blood Cells 14, (1988).

766. A rapid method for purification of organelles for DNA isolation: self-generated Percoll gradients.Pay, A. et al., Plant Cell Rep. 7, 96–98 (1988).

767. Use of Percoll density gradients for studying the attachment of bacteria to oral epithelial cells.Childs, W.C. et al., J. Dent. Res. 67, 826–830 (1988).

768. In vivo import of plastocyanin and a fusion protein into developmentally different plastidsof transgenic plants. de Boer, D. et al., EMBO J. 7, 2631–2635 (1988).

769. Studies on the capacity of intact cells and purified Ia from different B cell sources to functionin antigen presentation to T cells. Krieger, J. et al., J. Immunol. 140, 388–394 (1988).

770. Purification of brush border membrane vesicles from horse kidney cortex using Percoll.Boudouard, M. et al., Prep. Biochem. 18, 1–16 (1988).

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771. A rapid method for purification of organelles for DNA isolation: self-generated Percoll gradients.Pay, A. et al., Plant Cell Rep. 7, 96–99 (1988).

772. Harvesting and separation of two populations of lysosomes from porcine endometrium.Adamski, J. et al., Eur. J. Cell. Biol. 45, 238–245 (1987).

773. Isolation and characterization of rat trophoblast cells. Wang, K.-I. et al., Am. J. Reprod.Immunol. Microbiol. 16, 8–14 (1988).

774. Dopamine and serotonin in two populations of synaptosomes isolated by Percoll gradientcentrifugation. Robinson, P.J. and Lovenberg, W. Nevrochem. Int. 9, 455–458 (1986).

775. Separation of cell organelles in density gradients based on their permeability characteristics.Gasser, K.W. et al., Anal. Biochem. 171, 41–46 (1988).

776. Characterization of plasma membranes from A431 cells, isolated by self-generating Percollgradient: a rapid isolation procedure to obtain plasma membranes with functional epidermalgrowth factor receptors. Payrastre, B. et al., Biochim. Biophys. Acta. 939, 355–365 (1988).

777. Enrichment and selection of hybrid hybridomas by Percoll density gradient centrifugationand fluorescent activated cell sorting. Koolwijk, P. et al., Hybridoma 7, 217–225 (1988).

778. Attempted sexing of bovine spermatozoa by fractionation on a Percoll density gradient.Upreti, G.C. et al., Gamete Res. 20, 83–92 (1988).

779. Self-generating density gradients of Percoll provide a simple and rapid method that consistentlyenriches natural killer cells. Ravnik. S.E. et al., J. Immunol. Methods. 110, 161–168 (1988).

780. Cellular influx and activation increase macrophage cytotoxicity and interleukin-1 elaboratingduring pulmonary inflammation in rats. Fuchs, H.J., Sniezek, M. and Shellito, J.E. Am. Rev.Respir. Dis. 138, 572–577 (1988).

781. Stalk cell formation in monolayers from isolated prestalk prespore cells of Dictyosteliumdiscoideum: evidence for two populations of prestalk cells.Kwong, L. et al., Development104, 121–127 (1988).

782. Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulationsderived from MCF-7 breast carcinoma cells: cytogenetic analysis of the stem cell subpopulation.Podhajcer, O.L. et al., Exp. Cell Res. 179, 58–64 (1988).

783. Bovine peripheral blood leukocyte subpopulation dynamics following a primary bovineherpes virus-1 infection. Griebel, P.J. et al., Viral Immunol. 1, 267–286 (1987–1988).

784. Approaches to characterize by density-fractionation age-dependent properties of reticulocytemitochondria. Belkner, J. and Rapoport, S.M. Biomed. Biochim. Acta. 48, 3–11 (1989).

785. Characterization and reassembly of a regular array in the cell wall of Clostridium difficileGAI 4131. Masuda, K., Itoh, M. and Kawata, T. Microbiol. Immunol. 33, 287–298 (1989).

786. Rat brain synaptosomal ATP:AMP-phosphotransferase activity. Nagy, A.K., Shuster, T.A.and Delgado-Escueta, A.V. J. Neurochem. 53, 1166–1172 (1989).

787. Defective lysosomal egress of free sialic acid (N-acetylneuraminic acid) in fibroblasts ofpatients with infantile sialic acid storage disease. Tietze, F. et al., J. Biol. Chem. 264,15316–15322 (1989).

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788. Variation in buoyant density of whole cells and isolated cell wall of Streptococcus faecium(ATCC 9790). Glaser, D. et al., J. Bacteriol. 171, 4992–4995 (1989).

789. Phenotypic characterization of cynomolgus monkey natural killer cells. Yamada, Y.K., Yabe, M.and Tatsumi, M. Cell Immunol. 122, 524–533 (1989).

790. Correlation of membrane anisotropy with function in subpopulations of human blood platelets.Raha, S., Opper, C. and Wesemann, W. Br. J. Haematol. 72, 397–401 (1989).

791. Subcellular composition of platelet density subpopulations prepared using continuousPercoll gradients. Chamberlain, K.G. et al., Br. J. Haematology, 72, 199–207 (1989).

792. Occurrence in brain lysosomes of a sialidase active on ganglioside. Fiorilli, A. et al.,J. Neurochem. 53, 672–680 (1989).

793. Differential responsiveness of human B lymphocytes to phorbol ester and calcium ionophorebased on their state of activation. Inglés, J. et al., Immunology 67, 359–364 (1989).

794. Purification and culture of adult rat dorsal root ganglia neurons. Delree, P. et al.,J. Neurosci. Res. 23, 198–206 (1989).

795. Cytoplasmic bar-like structures of alveolar type II cells: an ultrastructural study in freshlyisolated cells from rat lungs. Vincent, R. and Nadeau, D. Am. J. Anat. 179, 70–78 (1987).

796. Small and large B cells respond differently to T cell-derived B cell growth and differentiationfactors. Layton, J.E. et al., J. Mol. Cell Immunol. 2, 155–167 (1985).

797. Lung cell reactions in guinea pigs after inhalation of asbestos (amosite). Sjöstrand, M.,Rylander, R. and Bergström, R. Toxicology, 57, 1–14 (1989).

798. The automated bone marrow micronucleus test. Romagna, F. and Staniforth, C.D.Mutat. Res. 213, 91–104 (1989).

799. Molecular mechanism for the inhibitory action of interferon on hematopoiesis. Orlic, D.et al., Ann. N.Y Acad. Sci. 554, 36–48 (1989).

800. Purification of mammalian cochlear hair cells using small volume Percoll density gradients.Holley, M.C. J. Neurosci. Methods 27, 219–224 (1988).

801. Functional differences of human basophils with different densities. Morita, Y. et al.,Int. Arch. Appl. Immunol. 88, 332–336 (1989).

802. Localization of thyroid hormone in subpopulations of rat alveolar macrophages. Liu, W.K.,Wong, C.C. and Mak, N.K. Histochem. J. 21, 115–121 (1989).

803. Characterization of g-glutamyl transpeptidase activity of cultured endothelial cells from porcinebrain capillaries. Mischeck, U., Meyer, J. and Galla, H.J. Cell Tissue Res. 256, 221–226 (1989).

804. The quantification of steroidogenesis-stimulating activity in testicular interstitial fluid by anin vitro bioassay employing adult rat Leydig cells. Hedger, M.P. et al., Endocrinology 127,1967–1977 (1990).

805. Characterization and analysis of soluble suppressor factor from early human decidual cells.Matsui, S., Yoshimura, N. and Oka, T. Transplantation 47, 678–683 (1989).

806. Isolation of plasma membranes from cultured glioma cells and application to evaluation ofmembrane sphingomyelin turnover. Cook, H.W. et al., Anal. Biochem. 174, 552–560 (1988).

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807. Biochemical analysis of the activation of adherent neutrophils in vitro. Cerasoli, Jr, F. et al.,Tissue and Cell 20, 505–517 (1988).

808. Synthesis of Factor VIII in human hepatocytes in culture. Ingerslev, J. et al., Thromb. Haemost.60, 387–391 (1988).

809. Prostaglandin E2 output by isolated rat gastric parietal cells and non-parietal epithelial cells.Ota, S. et al., Prostaglandins 36, 589–600 (1988).

810. Properties of high and low density subpopulations of group B streptococci: enhanced virulenceof the low density variant. Håkansson, S. et al., Microb. Pathogenesis 5, 345–355 (1988).

811. Subpopulations of human lung alveolar macrophages: ultrastructural features. Nakstad, B.et al., Ultrastruct. Pathol. 13, 1–13 (1989).

812. Hypodense eosinophilic granulocytes in normal individuals and patients with asthma:generation of hypodense cell populations in vitro. Kloprogge, E. et al., J. Allergy Clin.Immunol. 83, 393–400 (1989).

813. A modification of Reiber's laser-nephelometric protein determination method. Interferencesof selected substances. Himmel, H.M. and Heller, W. J. Clin. Chem. Clin. Biochem. 26,521–522 (1988).

814. Cytogenetic analysis of different cellular populations in chronic myelomonocytic leukemia.Cuneo, A. et al., Cancer. Genet. Cytogenet. 37, 29–37 (1989).

815. Rapid assay and identification of human haemopoietic growth factors. Pullman, W.E. et al.,J. Immunol. Methods 117, 153–161 (1989).

816. Heterogeneity of pituitary adenoma cell subpopulations from acromegalic patients obtainedby Percoll density gradient centrifugation.Hofland, L.J. et al., Acta Endocrinol. 121,270–278 (1989).

817. Determination of neutrophil concentration in semen by measurement of superoxide radicalformation. Kovalski, F. deLamirande, E. and Gagnon, C. J. Urol. 145, Suppl. (1991).

818. A single step centrifugation method for the enrichment of veiled cells from canine afferent lymph.Galkowska, H., Dabrowski, M. and Olszewski, W.L. J. Immunol. Methods 116, 207–212 (1989).

819. Macrophages phagocytose thymic lymphocytes with productively rearranged T cell receptora and b genes. Inaba, K. et al., J. Exp. Med. 168, 2279–2294 (1988).

820. Identification of a light density murine megakaryocyte progenitor (LD-CFU-M).Chatelain, C., DeBast, M. and Symann, M. Blood 72, 1187–1192 (1988).

821. Separation of rabbit epiphyseal chondrocytes in various stages of differentiation.Ralphs, J.R., Evans, L. and Ali, S.Y. Cell Tissue. Res. 254, 393–398 (1988).

822. Improved methods for separating hydrolytic granules of neutrophil leucocytes. Takano, Y.et al., Cell Biochem. Func. 6, 263–269 (1988).

823. Purification of plasma membrane from Acanthamoeba castellanii. Clarke, B.J., Hohman, T.C.and Bowers, B. J. Protozool. 35, 408–413 (1988).

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824. Isolation and culture of two cell populations from chick calvaria by density gradient:prostaglandin synthesis by the cells in culture and co-culture. Yang, C.Y. et al.,Endocrinology 123, 2549–2556 (1988).

825. Proline incorporation into protein by Rickettsia prowazekii during growth in Chinese hamsterovary (CHO-K1) cells. Austin, F.E., Winkler, H.H. Infect. Immun. 56, 3167–6172 (1988).

826. Human thymic dendritic cells. Characterization, isolation and functional assays. Landry, D.et al., Immunology 65, 135–142 (1988).

827. Identification of two subpopulations of thyroid lysosomes: relation to the thyroglobulinproteolytic pathway. Selmi, S. and Rousset, B. Biochem. J. 253, 523–532 (1988).

828. The production of mouse anti-Myxosoma cerebralis antiserum from Percoll-purified sporesand its use in immunoflourescent labelling of Historesin®-embedded cartilage derived frominfected rainbow trout, Salmo gairdneri. Richardson, J. Hamilton, A.J. and Canning, E.U.J. Fish Dis. 11, 185–190 (1988).

829. Comparison of different methods for the purification of eosinophils from human peripheral blood.Pretlow, T.P. et al., Anal. Bochem. 175, 334–341 (1988).

830. Improved separation of Rhesus monkey lymphocytes with Percoll. Budzko, D. et al.,Lab. Invest. 53, 586–588 (1985).

831. A study of adipocyte precursor cells derived from brown adipose tissue; the expression ofspecific cell surface antigens during their differentiation in culture. Lee, S.R. and Cryer, A. J. Dev. Phys. 13, 105–113 (1990).

832. Isolation of transcriptionally active nuclei from striated muscle using Percoll density gradients.Hahn, C, C. and Covault, J. Anal. Biochem. 190, 193–197 (1990).

833. Reticulocytes I. Isolation and in vitro maturation of synchronized populations. Noble, N.A.,Xu, Q. and Ward, J.H. Blood 74, 475–481(1989).

834. Heterogeneity of fractionated alveolar macrophages in starved rats. Sakai, K. et al.,Nutr. Res. 10, 1141–1149 (1990).

835. Localization of long-chain fatty acids and unconventional sterols in spherulous cells of a marinesponge. Lawson, M.P., Thompson, J.E. and Djerassi, C. Lipids 23, 1037–1048 (1988).

836. Monocyte-to-macrophage transition in vitro. A systematic study using human cells isolatedby fractionation on Percoll. Davies, D.E. and Lloyd, J.B. J. Immunol. Methods 118, 9–16 (1989).

837. Plasma membrane of cultured oligodendrocytes: I. isolation, purification and initialcharacterization. Polak, P.E. and Szuchet, S. GLIA 1, 39–53 (1988).

838. Trout Sertoli and Leydig cells: isolation, separation and culture. Loir, M. Gamete Res. 20,437–458 (1988).

839. Apparent densities of spermatozoa of various mammalian species. Oshio, S. Gamete Res. 20,159–164 (1988).

840. Properties of rabbit pepsinogen granules. Sharma, B.K., Gasser, K.W. and Hopfer, U.Gastroenterology 96, 1049–1057 (1989).

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841. Purification and restriction endonuclease analysis of plant nuclear DNA. Watson, J.C. andThomson, W.F. Methods Enzymol. 118, 57–75 (1986).

842. A method for isolation of nuclei containing undegraded RNA from RNAase-rich plasmodiaof Physarum polycephalum. Nothacker, K.D. and Hildebrandt, A. Biochim. Biophys. Acta950, 403–410 (1988).

843. Altered chromatin conformation of the higher plant gene phaseolin. Murray, M.G. andKennard, W.C. Biochemistry 23, 4225–4232 (1984).

844. A one-step technique for the subcellular fractionation of total cell homogenates. Morand, J.C.and Kent, C. Anal. Biochem. 159, 157–162 (1986).

845. Percoll centrifugation eliminates mold contaminants from cell culture. Kruk, P.A. andAuersperg, N. In vitro Cell. Dev. Biol. 27A, 273–276 (1991).

846. Semen manipulation: improved sperm recovery and function with a two-layer Percoll gradient.McClure, R.D., Nunes, L. and Tom, R. Fertil. Steril. 51, 874–877 (1989).

847. Motility and fertilizing ability of rat epididymal spermatozoa washed by a continuousgradient of Percoll. Miyake, M et al., Gamete Res. 24, 49–57 (1989).

848. Isolation of Cowdria ruminantium by means of Percoll density gradient centrifugation anddetection by an enzyme-linked immunosorbent assay. Neitz, A.W.H. et al., Onderstepoort J.Vet. Res. 53, 63–67 (1986).

849. Rapid isolation of bacteroids from soybean root nodules by Percoll discontinuous gradientcentrifugation. Kouchi, H. and Fukai, K. Soil Sci. Plant Nutr. 35, 301–305 (1989).

850. Use of a discontinuous Percoll gradient technique for the separation of channel catfish,Ictalurus punctatus (Rafinesque), peripheral blood leucocytes. Waterstrat, P.R., Ainsworth, A.J.and Capley, G. J. Fish Dis. 11, 289–294 (1988).

851. Separation of cytotoxin lymphocytes against Marek´s disease lymphoblastoid cell lines onPercoll gradients. Schat, K.A. and Shek. W.R. Avian Pathol. 13, 469–478 (1984).

852. Properties of Percoll-purified mitochondria from tubers of Manihot esculenta, a cyanogenic plant.Mbina, I., Rustin, P, and Lance, C. Plant Physol. Biochem. 29, 57–66 (1991).

853. The accuracy of sperm concentration determined by the automated CellSoft semen analyzerbefore and after discontinuous Percoll gradient centrifugation. Chan, S.Y.W. et al.,Andrologia 22, 55–61 (1989).

854. Plasmodium vivax: isolation of mature asexual stages and gametocytes from infected humanblood by colloidal silica (Percoll) gradient centrifugation. Ihalamulla, R.L. and Medis K.N.Trans. Roy. Soc. Trop. Med. Hyg. 81, 25–28 (1987).

855. A simple and reliable method for purification of Trichinella spiralis larvae using Percoll.Candolfi, E., France, P. and Kien, T. Trans. Roy. Soc. Trop. Med. Hyg. 82, 288 (1988).

856. Isolation of pure fractions of viable dorsal root ganglionic neurons from rabbit or mouseusing Percoll gradients. DeBoni, U. and Goldenberg, S.S.S. A Dissection and Tissue CultureManual of the Nervous System (Shahar, A. et al., Eds.) Alan R. Liss, Inc., pagen 230–232 (1989).

857. Comparison between a swim-up and a Percoll gradient technique of human spermatozoa.Åkerlof, E. et al., Int. J. Androl. 10, 663–669 (1987).

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858. Isolation of equine peripheral blood mononuclear cells using Percoll. May, S.A., Hooke, R.E.and Lees, P. Res. Vet. Sci. 50, 116–117 (1991).

859. Analysis of the relationship reactive oxygen species production and leucocyte infiltration infractions of human semen separated on Percoll gradients. Aitken, R.J. and West K.M.Int. J. Androl. 13, 433–451 (1990).

860. Self-generating density gradients of Percoll provide a simple and rapid method that consistentlyenriches natural killer cells. Ravik, S.E., and Pollack, S.B. J.Immunol. Meth. 110, 161–168 (1988).

861. Are human spermatozoa separated on a Percoll density gradient safe for therapeutic use?Pickering, S.J. et al., Fertil. Steril. 51, 1024–1029 (1989).

862. Evidence that Leydig precursers localize in immature band two cells isolated on Percoll gradients.Murono, E.P. and Washburn, A.L. J. Steroid Biochem. Molec. Biol. 37, 675–680 (1990).

863. Egg-penetration ability and structural properties of human sperm prepared by Percoll-gradient centrifugation. Tanphaichitr, N. et al., Gamete Res. 20, 67–81 (1988).

864. Isolation of pancreatic autophagic vaculoes induced by vinblastine or neutral red. Separationof autophagosomes and autolysosomes by Percoll density gradient centrifugation. Fellinger, E.and Réz, G. Eur. J. Cell Biol. 51, 220–228 (1990).

865. Isolation and purification of glandular secretory cells from Artemisia tridentata (ssp. vaseyana)by Percoll density gradient centrifugation. Slone, J.H. and Kelsey R.G. Amer. J. Bot. 72,1445–1451 (1985).

866. Prolactin cell subpopulations separated on discontinuous Percoll gradient: an immunocyto-chemical, biochemical, and physiological characterization. Velkeniers, B. et al.,Endocrinology 123, 1619–1630 (1988).

867. Bovine alveolar macrophages: phenotypic and functional properties of subpopulations obtainedby Percoll density gradient centrifugation. Ohmann, H.B. and Babiuk, L.A. J. Leuk. Biol.39, 167–181 (1986).

868. Separation of murine megakaryocytes and their progenitors on continuous gradients of Percoll.Ishibashi, T. and Burstein, S.A. J. Cell. Physiol. 125, 559–566 (1985).

869. Studies on conditions for cell division and embryogenesis in isolated pollen culture ofNicotiana rustica. Kyo, M. and Harada, H. Plant Physiol. 79, 90–84 (1985).

870. Isolation of hippocampal synaptosomes on Percoll gradients: cholinergic markers and ligandbinding sites. Thorne, B., Wonnacott, S. and Dunkley, P.R. J. Neurochem. 56, 479–484 (1991).

871. Rapid preparation of a distinct lysosomal population from myelinating mouse brain usingPercoll gradients. Caimi, L. et al., J. Neurochem. 52, 1722–1728 (1989).

872. Rapid isolation of metabolically active mitochondria from rat brain and subregions usingPercoll density gradient centrifugation. Sims, N.R. J. Neurochem. 55, 698–707 (1990).

873. Separation of human neutrophils in self-generated continuous density gradients of Percoll.Uyesaka, N., Tajiri, A. and Arai, K. Jap. J. Phys. 39, 629–634 (1989).

874. Removal of yeast contamination from lymphoblast cultures. Overhauser, J., Chakraborty, S.and Kelley-Card, L. Bio Techniques 8, 177 (1990).

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875. Separation of pre- and post-synaptic receptors on Percoll gradient. Wonnacott, S. andThorne, B. Biochem. Society Trans. 18, 885–886 (1990).

876. Nuclear maturity and morphology of human spermatozoa selected by Percoll density gradientcentrifugation or swim-up procedure. Le Lannou, D, and Blanchard, Y. J. Reprod. Fert. 84,551–556 (1988).

877. Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients.Kilani, R.T. and Sekla, L. Am. J. Trop. Med. Hyg. 36, 505–508 (1987).

878. Oxytocin in Leydig cells: an immunocytochemical study of Percoll-purified cells from rat testes.Yeung, W.S. et al., Cell Tissue Res. 253, 463–468 (1988).

879. Autoradiographical localization of luteinizing hormone releasing hormone (LHRH) receptorson rat testicular intertubular cells fractionated on Percoll density gradients.Aust. J. Biol. Sci. 38, 435-444 (1985).

880. Functional and morphological characterization of cultures of Küpffer cells and liver endothelialcells prepared by means of density separation in Percoll, and selective substrate adherence.Smedsrød, B. et al., Cell Tissue Res. 241, 639–649 (1985).

881. Sampling soil microfloras: optimization of density gradient centrifugation in Percoll to separatemicroorganisms from soil suspensions.MacDonald, R.M. Soil Biol. Biochem. 18, 407–410 (1986).

882. Multiple forms of rat prolactin and growth hormone in pituitary cell subpopulations separatedusing a Percoll gradient system: disulphide-bridged dimers and glycosylated variants.Bollengier, F. et al., J. Endocrinol. 120, 201-206 (1989).

883. An ultrastructural and functional characterization of rat somatotrophs highly enriched on acontinuous Percoll density gradient. Ohlsson, L., Lindström, P. and Norlund, R. Mol. Cell.Endocrinol. 59, 47–55 (1988).

884. Human peripheral blood and bone marrow cell separation using density gradient centrifugationon LymphoprepTM and Percoll in haematological diseases. Koistinen, P. Scand. J. Clin.Lab. Invest. 47, 709–714 (1987).

885. Natural killer cells in murine muscular dystrophy. Semple, J.W., Szewczuk, M.R.Clin. Immunol. Immunopath. 41, 116–129 (1986).

886. Long-term culture of rat mammotrope and somatotrope subpopulations separated oncontinuous Percoll density gradients: effect of dopamine, TRH, GHRH and somatostatin.Hofland, L.J. et al., Acta. Endocrinol. 122, 127–136 (1990).

887. Two fractions enriched for striatal synaptosomes isolated by Percoll gradient centrifugation:synaptosome morphology, dopamine and serotonin receptor distribution, and adenylatecyclase activity. Robinson, P.J. et al., Neurochem. Int. 15, 339–348 (1989).

888. Isolation of metabolically distinct synaptosomes on Percoll gradients. Sherman, A.D.Neurochem. Res. 14, 97–101 (1989).

889. Percoll-gradient separation of Leydig cells from postnatal rat testes. Risbridger, G.P. andde Kretser, D.M. J. Reprod. Fert. 76, 331–338 (1986).

890. Spermatozoa selection in discontinuous Percoll gradients for use in artificial insemination.Pardo, M. et al., Fertil. Steril. 49, 505–509 (1988).

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891. Down-regulation of L-selectin surface expression by various leukocyte isolation procedures.Stibenz, D. and Buhrer, C. Scand. J. Immunol. 39, 59–63 (1994).

892. Production of hematopoietic growth factors by human B lymphocytes: mechanisms andpossible implications. Pistoia, V., Corcione, A., Baldi, L. et al., Stem Cells 11, (suppl.2)150–155 (1993).

893. Differential expression of IL-4 receptors in human T and B lymphocytes. Mozo, L., Rivas, D.,Zamorano, J. et al., J. Immunol. 150, 4261–4269 (1993).

894. Isolation of functionally active intraepithelial lymphocytes and enterocytes from human smalland large intestine. Lundqvist, C., Hammarstrom, M.-L., Athlin, L. et al., J. Immunol.Methods 152, 253–263 (1992).

895. The presence of CD5LOW+NK cells in normal controls and patients with pulmonary tuberculosis.Ishiyama, T., Watanabe, K., Fukuchi, K. et al., Immunol. Lett. 37, 139–144 (1993).

896. Spontaneous production of granulocyte colony stimulating factor in vitro by human B-lineagelymphocytes is a distinctive marker of germinal center cells. Corcione, A., Baldi, L., Zupo, S.et al., J. Immunol. 153, 2868–2877 (1994).

897. Intestinal mucosal lymphocytes have H1 receptors: H1 antagonists reduce their proliferationand cytotoxicity. Roberts, A.I., Leone, V.M. and Ebert, E.C. Cell. Immunol. 156, 212–219 (1994).

898. The number of CD1a+ large low-density cells with dendritic cell features is increased in theperipheral blood of HIV+-patients. Ree, H.J., Liau, S., Yancovitz, S.R. et al., Clin. Immunol.Immunopathol. 70, 190–197 (1994).

899. Cell-to-cell mediated inhibition of natural killer cell proliferation by monocytes and itsregulation by histamine H2-receptors. Hellstrand, K. and Hermodsson, S. Scand. J. Immunol.34, 741–752 (1991).

900. Follicular dendritic cells inhibit human B lymphocyte proliferation. Freedman, A.S., Munro, J.M.,Rhynhart, K. et al., Blood 80, 1284–1288 (1992).

901. Constitutive expression and role in growth regulation of interleukin-1 and multiple cytokinereceptors in a biphenotypic leukemic cell line. Cohen, A., Grunberger, T., Vanek, W. et al.,Blood 78, 94–102 (1991).

902. Monoclonal anti-CD23 antibodies induce a rise in [Ca2+]i and polyphosphoinositidehydrolysis in human activated B cells. Kolb, J.-P., Renard, D., Dugas, B. et al., J. Immunol.145, 429–437 (1990).

903. Interleukin-2-inducible killer activity and its regulation by blood monocytes from autologouslymphocytes of lung cancer patients. Sone, S., Kunishige, E., Fawzy, F. et al., Jpn. J. Cancer Res.82, 716–723 (1991).

904. Lymphokine-activated killer cell regulation of T-cell mediated immunity to Candida albicans.Wei, S., Blanchard, D.K., McMillen, S. et al., Infect. Immun. 60, 3586–3595 (1992).

905. Coregulation of the APO-1 antigen with intercellular adhesion molecule-1 (CD54) intonsillar B cells and coordinate expression in follicular center B cells and in follicle centerand mediastinal B-cell lymphomas. Moller, P., Henne, C., Leithauser, F. et al., Blood 81,2067–2075 (1993).

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906. Interleukin-2 activated T cells (T-LAK) express CD16 antigen and are triggered to target cell lysisby bispecific antibody. Nitta, T., Nakata, M., Yagita, H. et al., Immunol. Lett. 28, 31–38 (1991).

907. Antitumor activity, growth, and phenotype of longterm IL-2 cultures of human NK and Tlymphocytes. Fuchshuber, P.R., Lotzová, E., Pollock, R.E. Lymphokine and Cytokine Res.10, 51–59 (1991).

908. Functional characterization of canine lymphocyte subsets. Hotzl, C., Kolb, H.J., Holler, E.et al., Ann. Hematol. 63, 49–53 (1991).

909. Measurement of NK activity in effector cells purified from canine peripheral lymphocytes.Knapp, D.W., Leibnitz, R.R., DeNicola, D.B. et al., Vet. Immunol. Immunopathol. 35,239–251 (1993).

910. A rapid method for isolating murine intestine intraepithelial lymphocytes with high yieldand purity. Mosley, R.L. and Klein, J.R. J. Immunol. Meth. 156, 19–26 (1992).

911. Differential sensitivity of virgin and memory T lymphocytes to calcium ionophores suggestsa buoyant density separation method and a model for memory cell hyporesponsiveness to Con A.Miller, R.A., Flurkey, K., Molloy, M. et al., J. Immunol. 147, 3080–3086 (1991).

912. Characterization of protein phosphorylation by 2,3,7,8,-tetrachlorodibenzo-para-dioxin inmurine lymphocytes: indirect evidence for a role in the suppression of humoral immunity.Snyder, N.K., Kramer, C.M., Dooley, R.K. et al., Drug and Chem. Toxicol. 16, 135-163 (1993).

913. Cytokine synthesis by intestinal intraepithelial lymphocytes. Yamamoto, M., Fujihashi, K.,Beagley, K.W. et al., J. Immunol. 150, 106–114 (1993).

914. The heavy metal lead exhibits B cell-stimulatory factor activity by enhancing B cell Ia expressionand differentiation. McCabe, Jr., M.J. and Lawrence, D.A. J. Immunol. 145, 671–677 (1990).

915. Antibacterial activity of bovine mammary grand lymphocytes following treatment withinterleukin-2 J. Sordillo, L.M., Campos, M. and Babiuk, L.A. Dairy Sci. 74, 3370–3375 (1991).

916. Use of probe to repeat sequence of the Y chromosome for detection of host cells in peripheralblood of bone marrow transplant recipients. Przepiorka, D., Thomas, E.D., Durnamn, D.M.et al., Am. J. Clin. Pathol. 95, 201–206 (1991).

917. Differences in "antioncogene" p53 expression in human monocytes and lymphocytes in vitro.Osipovich, O.A., Sudarikov, A.B., Kolesnikova, T.S. et al., Biull. Eksp. Biol. Med. 113,638–640 (1992).

918. Regulation of tumor necrosis factor-alpha production and gene expression in monocytes.Kohn, F.R., Phillips, G.L. and Klingemann, H.-G. Bone Marrow Transplantation 9,369–376 (1992).

919. Killing of Coccidioides immitis by human peripheral blood mononuclear cells. Ampel, N.M.,Bejarano, G.C. and Galgiani, J.N. Infect. Immun. 60, 4200–4204 (1992).

920. Monocyte dysfunction in patients with Gaucher disease: evidence for interference ofglucocerebroside with superoxide generation. Liel, Y., Rudich, A., Nagauker-Shriker, O. et al.,Blood 83, 2646–2653 (1994).

921. Effect of human immunodeficiency virus-1 envelope glycoprotein on in vitro hematopoiesisof umbilical cord blood. Sugiura, K., Oyaizu, N., Pahwa, R. et al., Blood 80, 1463–1469 (1992).

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922. Point mutations in the beta-subunit of cytochrome b558 leading to X-linked chronicgranulomatous disease. Bolscher, B.G.J.M., de Boer, M., de Klein, A. et al., Blood 77,2482–2487 (1991).

923. Acceleration of chronic myeloid leukemia correlates with calcitonin gene hypermethylation.Malinen, T., Palotie, A., Pakkala, S. et al., Blood 77, 2435–2440 (1991).

924. Expression of c-jun protooncogene in human myelomonocytic cells. Bertani, A.,Polentarutti, N., Sica, A. et al., Blood 74, 1811–1816 (1989).

925. Expression of a heat-inducible gene of the HSP70 family in human myelomonocytic cells:regulation by bacterial products and cytokines. Fincato, G., Polentarutti, N., Sica, A. et al.,Blood 77, 579–586 (1991).

926. Minimal residual disease is more common in patients who have mixed T-cell chimerismafter bone marrow transplantation for chronic myelogenous leukemia. MacKinnon, S.,Barnett, L., Heller, G. et al., Blood 83, 3409–3416 (1994).

927. Expression of C-myb and B-myb, but not A-myb, correlates with proliferation in humanhematopoietic cells. Golay, J., Capucci, A., Castellano, M. et al., Blood 77, 149–158 (1991).

928. Interactions between the monocyte/macrophage and the vascular smooth muscle cell. Zhang, H.,Downs, E.C., Lindsey, J.A. et al., Arteriosclerosis and Thrombosis 13, 220–230 (1993).

929. Splice site mutations are common cause of X-linked chronic granulomatous disease. de Boer, M.,Bolscher, B.G.J.M., Dinauer, M.C. et al., Blood 80, 1553–1558 (1992).

930. Isolation of equine peripheral blood mononuclear cells using Percoll. May, S.A., Hooke, R.E.and Lees, P. Res. Vet. Sci. 50, 116–117 (1991).

931. Proteolytic cleavage of CR1 on human erythrocytes in vivo: evidence for enhanced cleavagein AIDS. Pascual, M., Danielsson, C., Steiger, G. et al., Eur. J. Immunol. 24, 702–708 (1994).

932. Lipid peroxidation in Plasmodium falciparum-parasitized human erythrocytes. Simoes, A.P.C.F.,van den Berg, J.J.M., Roelofsen, B. et al., Arch. Biochem. Biophys. 298, 651–657 (1992).

933. Clinical utility of fractionating erythrocytes into Percoll density gradients. Mosca, A.,Paleari, R., Modenese, A. et al., Red Blood Cell Aging 227–238 (1991).

934. A monoclonal antibody monitoring band 3 modifications in human red blood cells.Giuliani, A., Marini, S., Ferroni, L. et al., Molec. Cell. Biochem. 117, 43-51 (1992).

935. Deoxygenation-induced changes in sickle cell-sickle cell adhesion. Morris, C.L., Rucknagel, D.L.and Joiner, C.H. Blood 81, 3138–3145 (1993).

936. Density-associated changes in platelet-activating factor acetylhydrolase activity and membranefluidity of human erythrocytes. Yoshida, H., Satoh, K., Ishida, H. et al.,Ann. Hematol. 69, 139–146 (1994).

937. Density gradients separation of L-asparaginase-loaded human erythrocytes. Garin, M.I.,Kravtzoff, R., Chestier, N. et al., Biochem. Mol. Biol. International 33, 807–814 (1994).

938. Determinations of red blood cell deformability in relation to cell age. Bosch, F.H., Werre, J.M.,Schipper, L. et al., Eur. J. Haematol. 52, 35–41 (1994).

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939. Density gradient separation of inositol hexaphosphate loaded in red blood cells in variouspreparation conditions. Bourget, G., Boucher, L. and Ropars, C, In The Use of ResealedErythrocytes as Carriers and Bioreactors. (Magnani, M. and DeLoach, J.R., Eds.)Plenum Press, NY 27–33 (1992).

940. IHP entrapment into human erythrocytes: comparison between hypotonic dialysis andDMSO osmotic pulse. Mosca, A., Paleari, R., Russo, V. et al., In The Use of ResealedErythrocytes as Carriers and Bioreactors, (Magnani, M. and DeLoach, J.R., Eds.)Plenum Press, NY 19–26 (1992).

941. Cyclic, AMP level in red blood cells of Plasmodium berghei-infected Mastomys natalensis.Khare, S. and Ghatak, S. Experientia 47, 236-238 (1991).

942. Exposure of phosphatidylserine in the outer leaflet of human red blood cells. Connor, J.,Pak, C.C. and Schroit, A.J. J. Biol. Chem. 269, 2399–2404 (1994).

943. Flow cytometric detection of micronuclei induced by chemicals in poly- and normochromaticerythrocytes of mouse peripheral blood. Cao, J., Beisker, W., Nusse, M. et al., Mutagenesis 8,533–541 (1993).

944. Temporal replacement of donor erythrocytes and leukocytes in nonanemic W44J/W44J andseverely anemic W/W+ mice. Barker, J.E., Greer, J., Bacon, S. et al., Blood. 78, 1432–1437 (1991).

945. Oxygen transport properties in malaria-infected rodents - a comparison between infected andnoninfected erythrocytes. Schmidt, W., Correa, R., Boning, D. et al., Blood 83, 3746–3752 (1994).

946. Multicatalytic and 26S ubiquitin/ATP-stimulated proteases in maturing rabbit red blood cells.Di Cola, D., Pratt, G. and Rechsteiner, M. FEBS 280, 137–140 (1991).

947. Biochemical characterization of density-separated trout erythrocytes. Falcioni, G., Grelloni, F.,Bonfigli, A.R. et al., Biochem. International 28, 379–384 (1992).

948. Loss of activation-induced CD45RO with maintenance of CD45RA expression during prolongedculture of T cells and NK cells. Warren, H.S. and Skipsey, L.J. Immunol. 74, 78–85 (1991).

949. Negative modulation of human NK cell activity by purinoreceptors: Effect on exogenousadenosine triphosphate. Krishnaraj, R. Cell. Immunol. 141, 306–322 (1992).

950. Lysis of neuroblastoma cell lines by human natural killer cells activated by interleukin-2 andinterleukin-12. Rossi, A.R., Pericle, F., Rashleigh, S. et al., Blood 83, 1323–1328 (1994).

951. Natural killer-stimulatory effect of combined low-dose interleukin-2 and interferon beta inhairy-cell leukemia patients. Liberati, A.M., De Angelis, V., Fizzotti, M. et al., CancerImmunol. Immunother. 38, 323–331 (1994).

952. Mouse hypersensitivity pneumonitis: depletion of NK cells abrogates the spontaneous regressionphase and leads to massive fibrosis. Denis, M. Exp. Lung Res. 18, 761–773 (1992).

953. Natural cytotoxic T cells responsible for anti-CD3-induced cytotoxicity in mice. Yanagita, Y.,Nishimura, T., Goa, X. et al., Immunol. Lett. 31, 137–142 (1992).

954. Generation of natural killer cells from both FcgRII/III+ and FcgRII/III- murine fetal liverprogenitors. Moingeon, P., Rodewald, H.-R., McConkey, D. et al., Blood 82, 1453–1462 (1993).

955. Hypoxic human umbilical vein endothelial cells induce activation of adherent polymorphonuclearleukocytes. Arnould, T., Michiels, C. and Remacle, J. Blood 83, 3705–3716 (1994).

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956. Platelet-activating factor-induced polymorphonuclear neutrophil priming independent ofCD11B adhesion. Read, R.A., Moore, E.E., Moore, F.A. et al., Surgery 114, 308–313 (1993).

957. Human neutrophils synthesize thrombomodulin that does not promote thrombin-dependentprotein C activation. Conway, E.M., Nowakowski, B. and Steiner-Mosonyi, M. Blood 80,1254–1263 (1992).

958. Induction of low density an up-regulation of CD11b expression of neutrophils and eosinophilsby dextran sedimentation and centrifugation. Berends, C., Dijkhuizen, B., de Monchy, J.G.R.et al., J. Immunol. Meth. 167, 183–193 (1994).

959. Intracellular localization of glycosyl-phosphatidyl-inositol-anchored CD67 and FcRIII(CD16) in affected neutrophil granulocytes of patients with paroxysmal nocturnalhemoglobinuria. Jost, C.R., Gaillard, M.L., Fransen, J.A.M. et al., Blood 78, 3030–3036 (1991).

960. Membrane surface antigen expression on neutrophils: a reappraisal of the use of surfacemarkers for neutrophil activation. Kuijpers, T.W., Tool, A.T.J., van der Schoot, C.E. et al.,Blood 78, 1105–1111 (1991).

961. Identification of neutrophil gelatinase-associated lipocalin as a novel matrix protein ofspecific granules in human neutrophils. Kjeldsen, L., Bainton, D.F., Sengelov, H. et al.,Blood 83, 799–807 (1994).

962. RANTES- and interleukin-8-induced responses in normal human eosinophils: effects ofpriming with interleukin-5. Schweizer, R.C., Welmers, B.A.C., Raaijmakers, J.A.M. et al.,Blood 83, 3697–3704 (1994).

963. Human platelets secrete chemotactic activity for eosinophils.Burgers, J.A., Schweizer, R.C.,Koenderman, L. et al., Blood. 81, 49–55 (1993).

964. Granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), and IL-5 greatlyenhance the interaction of human eosinophils with opsonized particles by changing theaffinity of complement receptor type-3. Blom, M., Tool, A.T.J., Kok, P.T.M. et al., Blood 83,2978–2984 (1994).

965. Modulation and induction of eosinophil chemotaxis by granulocyte-macrophage colony-stimulating factor and interleukin-3. Warrings, R.A.J., Koenderman, L., Kok, P.T.M. et al.,Blood 77, 2694–2700 (1991).

966. Migration of primed human eosinophils across cytokine-activated endothelial cell monolayers.Moser, R., Fehr, J., Olgiati, L. et al., Blood 79, 2937–2945 (1992).

967. Purification of human basophils by density and size alone. Kepley, C., Craig, S. andSchwartz, L. J. Immunol. Meth. 175, 1–9 (1994).

968. Purification of normal human bone-marrow-derived basophils. Arock, M., Mossalayi, M.D.,Le Goff, L. et al., Int. Arch. Allergy Immunol. 102, 107–111 (1993).

969. Effects of cytokines on human basophils chemotaxis. Tanimoto, Y., Takahashi, K. andKimura, I. Clin. Exper. Allergy 22,1020–1025 (1992).

970. Purification of human blood basophils by negative selection using immunomagnetic beads.Bjerke, T., Nielsen, S., Helgestad, J. et al., J. Immunol. Meth. 157, 49–56 (1993).

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971. Purification of human blood basophils using negative selection by flow cytometry.Tanimoto, Y., Takahashi, K., Takata, M. et al., Clin. Exper. Allergy 22, 1015–1019 (1992).

972. Purification of human blood basophils and leukotriene C4 generation following calciumionophore stimulation. Mita, K., Akiyama, K., Hayakawa, T. et al., Prostaglandins,Leukotrienes and Essential Fatty Acids 49, 783–788 (1993).

973. Effect of nerve growth factor on the release of inflammatory mediators by mature humanbasophils. Bischoff, S. and Dahinden, C.A. Blood 79, 2662–2669 (1992).

974. Infection of Nippostrongylus brasiliensis induces normal increase of basophils in mast cell-deficientWs/Ws rats with a small deletion of the kinase domain of c-kit. Kasugai, T., Okada, M.,Morimoto, M. et al., Blood 81, 2521–2529 (1993).

975. Thawed human hepatocytes in primary culture. Dou, M., de Sousa, G., Lacarelle, B. et al.,Cryobiol. 29, 454–469 (1992).

976. The distribution of non-specific carboxylesterases and glutathione S-transferases in differentrat liver cells. Gad, M.Z. Biochem. Pharmacol. 48, 139–144 (1994).

977. Methods in laboratory investigation: assessment of a method of isolation, purification, andcultivation of rat liver sinusoidal endothelial cells. Braet, F., De Zanger, R., Sasaoki, T. et al.,Lab. Invest. 70, 944–952 (1994).

978. Differential expression of platelet-derived growth factor a- and b-receptors on fat-storingcells and endothelial cells of rat liver. Heldin, P., Pertoft, H., Nordlinger, H. et al., Exper.Cell Res. 193, 364–369 (1991).

979. Preservation of the rate and profile of xenobiotic metabolism in rat hepatocytes stored inliquid nitrogen. Zaleski, J., Richburg, J. and Kauffman, F.C. Biochem. Pharmacol. 46,111-116 (1993).

980. Inhibition of biotransformation of xenobiotic p-nitroanisole after cryopreservation ofisolated rat hepatocytes. Petrenko, A.Y. and Mazur, S.P. Cryobiol. 30, 158-163 (1993).

981. A method for the cryopreserved rat liver parenchymal cells for studies of xenobiotics.Diener, B., Utesch, D., Beer, N. et al., Cryobiol. 30, 116–127 (1993).

982. Characterization of cryopreserved rat liver parenchymal cells by metabolism of diagnosticsubstrates and activities of related enzymes. Utesch, D., Diener, B., Molitor, E. et al.,Biochem. Pharmacol. 44, 309–315 (1992).

983. Salmonella choleraesuis and Salmonella typhimurium associated with liver cells after intravenousinoculation of rats are localized mainly in Küpffer cells and multiply intracellularly.Nnalue, N.A., Shnyra, A., Hultenby, K. et al., Infect. Immun. 60, 2758–2768 (1992).

984. Erythroid colony formation by fetal rat liver and spleen cells in vitro: inhibition by a lowrelative molecular mass component of fetal spleen. Nagel, M.-D. and Nagel, J. Development114, 213–219 (1992).

985. Fate of DNA targeted to the liver by asialoglycoproein receptor-mediated endocytosis in vivo.Chowdhury, N.R., Wu, C.H., Wu, G.Y. et al., J. Biol. Chem. 268, 11265–11271 (1993).

986. Epidermal growth factor stimulates testosterone production of human Leydig cells in vitro.Syed, V., Khan, S.A. and Nieschlag, E. J. Endocrinol. Invest. 14, 93–97 (1991).

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987. Immunocytochemical localization and endogenous synthesis of apolipoprotein E in testicularLeydig cells. Schleicher, R.L., Zheng, M. and Zhang, M. Biol. Reprod. 48, 313–324 (1993).

988. Isolation of human Leydig cell mesenchymal precursors from patients with the androgeninsensitivity syndrome: testosterone production and response to human chorionic gonadotropinstimulation in culture. Chemes, H., Cigorraga, S., Bergada, C. et al., Biol. Reprod. 46,793–801 (1992).

989. Enhancement of testosterone secretion by normal adult human Leydig cells by co-culturewith enriched preparations of normal adult human Sertoli cells. Lejeune, H., Skalli, M.,Sanchez, P. et al., International. J. Androl. vol 4, 27–34 (1993).

990. Heterogeneity of adult mouse Leydig cells with different buoyant densities.Chamindrani Mendis-Handagama, S.M.L. and De Kretser, D.M. J. Androl. 13, 274–282 (1992).

991. Effect of cortisol on testosterone production by immature pig Leydig cells. Li, P.S. J. SteroidBiochem. Molec. Biol. 38, 205–212 (1991).

992. Insulin-like growth factor-binding protein-2: the effect of human chorionic gonadotropin onits gene regulation and protein secretion and its biological effect in rat Leydig cells.Wang, D., Nagpal, M.L., Lin, T. et al., Mol. Endocrinol. 8, 69–76 (1994).

993. Germ cell localization of a testicular growth hormone-releasing hormone-like factor.Srivastava, C.H., Collard, M.W., Rothrock, J.K. et al., Endocrinol. 133, 83–89 (1993).

994. Separation and characterization of Leydig cells and macrophages from rat tests. Dirami, G.,Poulter, L.W. and Cooke, B.A. J. Endocrinol. 130, 357–365 (1991).

995. D5-3b-hydroxysteroid dehydrogenase-isomerse activity in two distinct density Leydig cellsfrom immature rats. Differences in responsiveness to human chorionic gonadotropin or8-bromoadensoine 3',5'-monophosphate. Murono, E.P. and Washburn, A.L. Biochem.Biophys. Acta 1091, 55–62 (1991).

996. A possible role for a low molecular weight peptide in regulation of testosterone productionby rat Leydig cells. Ramaraj, P., Subbarayan, V.S.R. and Jagannadha, R.A. Indian J. Biochem.Biophys. 28, 536–540 (1991).

997. Interleukin-1a-induced changes in androgen and cyclic adenosine 3',5'- monophosphate releasein adult rat Leydig cells in culture. Moore, C. and Moger, W.H. J. Endocrinol. 129,381–390 (1991).

998. Enhanced stimulation of 5a-reductase activity in cultured Leydig cell precursors by humanchorionic gonadotropin. Murono, E.P., Washburn, A.L. and Goforth, D.P. J. Steroid Biochem.Molec. Biol. 48, 377–384 (1994).

999. Rat seminiferous tubular culture medium contains a biological factor that inhibits Leydigcells steroidogenesis; its purification and mechanism of action. Zwain, I.H. and Cheng, C.Y.Mol. Cell. Endocrinol. 104, 213–227 (1994).

1000. In vitro DNA synthesis in Leydig and other interstitial cells of the rat testis. Moore, A.,Findlay, K. and Morris, I.D. J. Endocrinol. 124, 247–255 (1992).

1001. Flow cytometric comparison between swim-up and Percoll gradient techniques for theseparation of frozen-thawed human spermatozoa. Chen, Y., Obhrai, M.S., Chapman, J. et al.,Int. J. Fertil. 37, 315–319 (1992).

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1002. Comparative evaluation of three sperm-washing methods to improve sperm concentrationand motility in frozen-thawed oligozoospermic and normozoospermic samples. Bongso, A.,Jarina, A.K., Ho, J. et al., Archiv. Androl. 31, 223–230 (1993).

1003. Human spermatozoa selected by Percoll gradient or swim-up are equally capable of bindingto the human zona pellucida and undergoing the acrosome reaction. Morales, P., Vantman, D.,Barros, C. et al., Human Reprod. 6, 401–404 (1991).

1004. Isolation of motile spermatozoa: comparison of Percoll centrifugation, SpermPrep filtration,and swim-up techniques. Ziebe, S. and Andersen, C.Y. J. Assist. Repro. Genet. 10,485–587 (1993).

1005. Adenosine triphosphate and motility characteristics of fresh and cryopreserved humanspermatozoa. McLaughlin, E.A., Ford, W.C.L. and Hull, M.G.R. International. J. Androl.17, 19–23 (1994).

1006. Association of human sperm nuclear condensation and in vitro penetration ability.Chan, P.J. and Tredway, D.R. Andrologia. 24, 77–81 (1992).

1007. Differential sperm performance as judged by the zonafree hamster egg penetration testrelative to differing sperm penetration test relative to differing sperm penetration techniques.Chan, S.Y.W. and Tucker, M.J. Human Reprod. 7, 255–260 (1992).

1008. Effect on swim-up, Percoll and Sephadex sperm separation methods on the hypo-osomoticswelling test. Check, J.H., Katsoff, D., Kozak, J. et al., Human Reprod. 7, 109–111 (1992).

1009. Comparison of characteristics of human spermatozoa selected by the multiple-tube swim-upand simple discontinuous Percoll gradient centrifugation. Chan, S.Y.W, Chan. Y.M. andTucker, M.J. Andrologia 23, 213–218 (1991).

1010. Inhibition of the acrosome reaction by trypsin inhibitors and prevention of penetration ofspermatozoa through the human zona pellucida. Llanos, M., Vigil, P., Salgado, A.M. et al.,J. Reprod. Fertil. 97, 173–178 (1993).

1011. Sperm recovery techniques to maximize fertilizing capacity. Mortimer, D. Reprod. Fertil. Dev.6, 25–31 (1994).

1012. Human zona pellucida recognition associated with removal of sialic acid human sperm surface.Lassalle, B. and Testart, J. J. Reprod. Fertil. 101, 703–711 (1994).

1013. Relationship between iron-catalysed lipid peroxidation potential and human sperm function.Aitken, R.J., Harkiss, D. and Buckingham, D. J. Reprod. Fertil. 98, 257–265 (1993).

1014. Leukocytic infiltration into the human ejaculate and its association with semen quality,oxidative stress, and sperm function. Aitken, R.J., West, K. and Buckingham, D. J. Androl.15, 343–352 (1994).

1015. Simultaneous measurement of sperm LDH, LDH-X, CPK activities and ATP content innormospermic and oligozoospermic men. Orlando, C., Krausz, C., Forti, G. et al.,International. J. Androl. 17, 13–18 (1994).

1016. Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygenspecies in the ejaculate of oligozoospermic patients and fertile donors. Aitken, R.J.,Buckingham, D., West, K. et al., J. Reprod. Fert. 94, 451–462 (1992).

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1017. Analysis of the extent to which sperm movement can predict the results of ionophore-enhancedfunctional assays of the acrosome reaction and sperm-oocyte fusion. Aitken, J., Buckingham, D.and Harkiss, D. Human Reprod. 9, 1867–1874 (1994).

1018. Density differences between spermatozoa with antisperm autoantibodies and spermatozoacovered with antisperm antibodies from serum. Almagor, M., Margalioth, E.J. and Yaffe, H.Human Reprod. 7, 959–961 (1992).

1019. Movement characteristics of human spermatozoa collected from different layers of adiscontinuous Percoll gradient. Saad, A. and Guerin, J.E. Andrologia 24, 149–153 (1992).

1020. Comparison of the chromatin stainability of human spermatozoa separated by discontinuousPercoll gradient centrifugation: a flow cytometric contribution. Pasteur, X., Maubon, I.,Sabido, O. et al., Anal. Quant. Cytol. Histol. 14, 96–104 (1992).

1021. Selection of human spermatozoa by a hyperosmotic two-layer Percoll gradient. Chan, Y.-M.Abuzeid, M.I., Malcomnson, J.H. et al., Fertil. Steril. 61, 1097–1102 (1994).

1022. Ultrastructural comparison of human spermatozoa along a Percoll density gradient.Barthelemy, C., Fricot, G., Hamamah, S. et al., Int. J. Fertil. 37, 362–367 (1992).

1023. Flow cytometric sorting of living, highly motile human spermatozoa based on evaluation oftheir mitochondrial. Auger, J., Leonce, S., Jouannet, P. et al., J. Histochem. Cytochem. 41,1247–1251 (1993).

1024. Evaluating acrosome reaction steps with brightfield and differential interference contrastmicroscopy techniques. Steinholt, H.C., Chandler, J.E. and Tirado, V. J. Dairy Sci. 74,3822–3826 (1991).

1025. Lipid composition of hamster epididymal spermatozoa. Awano, M., Kawaguchi, A. andMohri, H. J. Reprod. Fertil. 99, 375–383 (1993).

1026. Separate effects of caffeine and dbcAMP on macaque sperm motility and interaction withthe zona pellucida. Vandevoort, C.A., Tollner, T.L. and Overstreet, J.W. Mol. Reprod. Dev.37, 299–304 (1994).

1027. Effects of the stem cell factor, c-kit ligand, on human megakaryocytic cells. Avraham, H.,Vannier, E., Cowley, S. et al., Blood 79, 365–371 (1992).

1028. Interferon-a downregulates the abnormal intracytoplasmic free calcium concentration oftumor cells in hairy cell leukemia. Genot, E., Bismuth, G., Degos, L. et al., Blood 80,2060–2065 (1992).

1029. Infection of megakaryocytes by human immunodeficiency virus in seropositive patients withimmune thrombocytopenic purpura. Louache, F., Bettaieb, A., Henri, A. et al., Blood. 78,1697–1705 (1991).

1030. Fibrinogen g-chain mRNA is not detected in human megakaryocytes. Lange, W., Luig, A.,Dolken, G. et al., Blood 78, 20–25 (1991).

1031. Growth and detection of human bone marrow B lineage colonies. McGinnes, K., Keystone, E.,Bogoch, E. et al., Blood 76, 896–905 (1990).

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1032. Myeloid and lymphoid chimerism after T cell-depleted bone marrow transplantation:evaluation of conditioning regiments using the polymerase chain reaction to amplify humanminisatellite regions of genomic DNA. MacKinnon, S., Barnett, L., Bourhis, J.H. et al.,Blood 80, 3235–3241 (1992).

1033. T-cell subsets and suppressor cells in human bone marrow. Schmidt-Wolf, I.G.H.,Dejbakhsh-Jones, S., Ginzton, N. et al., Blood 80, 3242–3250 (1992).

1034. The heparin binding PECAM-1 adhesion molecule is expressed by CD34+ hematopoieticprecursor cells with early myeloid and B-lymphoid cell phenotypes.Watt, S.M., Williamson, J.,Genevier, H. et al., Blood 82, 2649–2663 (1993).

1035. Long-term interleukin-6 administration stimulates sustained thrombopoiesis and acute-phaseprotein synthesis in a small primate - the marmoset. Ryffel, B., Car, B.D., Woerly, G. et al.,Blood 83, 2093–2102 (1994).

1036. Effect of human interleukin-6 on megakaryocyte development and thrombocytopoiesis inprimates. Stahl, C.P., Zucker-Franklin, D., Evatt, B.L. et al., Blood 78, 1467–1475 (1991).

1037. Studies of an improved Rhesus hematopoietic progenitor cell assay. Winton, E.F., Jacobs, P.C.,Rozmiarek, S.K. et al., Exp. Hematol. 20, 401–404 (1992).

1038. Interleukin-10 inhibits the osteogenic activity of mouse bone marrow. Van Vlasselaer, P.,Borremans, B., Van Den Heuvel, R. et al., Blood 82, 2361–2370 (1993).

1039. Interleukin-3 and lipopolysaccharide interact to inhibit proliferation to mouse bone marrow cells.Silva, Z.Z.A., Furlanetto, M.P., Marques, E.K. et al., Immunol. Lett. 40, 55–58 (1993).

1040. Separation of hematopoietic stem cells into two populations and their characterization.Ogata, H., Taniguchi, S., Inaba, M. et al., Blood 80, 91–95 (1992).

1041. Production of hematopoietic stem cell-chemotactic factor by bone marrow stromal cells.Cherry, B., Yasumizu, R., Toki, J. et al., Blood 83, 964–971 (1994).

1042. Characterization of bone cells isolated on discontinuous Percoll gradients: distribution insequentially derived populations. Wong, G.L., Ng, M.C., Calabrese, D.W. et al., J. Bone andMineral Res. 6, 969–976 (1991).

1043. LPS activation of bone marrow natural suppressor cells. Holda, J.H. Cell. Immunol. 141,518–527 (1992).

1044. Enrichment and functional characterization of Sca-1+WGA+, Lin-WGA+, Lin-Sca-1+, andLin-Sca-1+WGA+ bone marrow cells from mice with an Ly-6a haplotype. Jurecic, R., Van, N.T.and Belmont, J.W. Blood 82, 2673–2683 (1993).

1045. Different subsets of T cells in the adult mouse bone marrow and spleen induce or suppressacute graft-versus-host disease. Palathumpat, V., Dejbakhsh-Jones, S., Holm, B. et al.,J. Immunol. 149, 808–817 (1992).

1046. Purification of rat megakaryocyte colony-forming cells using a monoclonal antibody againstrat platelet glycoprotein IIb/IIIa. Miyazaki, H., Inoue, H., Yanagida, M. et al., Exp. Hematol.20, 855–861 (1992).

1047. Hematopoiesis in asymptomatic cats infected with feline immunodeficiency virus.Linenberger, M.L., Shelton, G.H., Persik, M.T. et al., Blood 78, 1963–1968 (1991).

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1048. Enhanced reactive oxygen species metabolism of air space cells in hypersensitivity pneumonitis.Calhoun, W.J. J. Lab. Clin. Med. 117, 443–52 (1991).

1049. Alveolar macrophage subpopulations in patients with active pulmonary tuberculosis.Kuo, H.-P. and Yu, C.-T. Chest 103, 1773–1778 (1993).

1050. Enhanced superoxide production by alveolar macrophages and air-space cells, airwayinflammation, and alveolar macrophage density changes after segmental antigenbronchoprovocation in allergic subjects. Calhoun, W.J., Reed, H.E., Moest, D.R. et al.,Am. Rev. Respir. Dis. 145, 317–325 (1992).

1051. Secretion of platelet-activating factor acetylhydrolase by human decidual macrophages.Narahara, H., Nishioka, Y. and Johnston, J.M. J. Clin. Endocrinol. Metab. 77,1258–1262 (1993).

1052. Airway macrophages from patients with asthma do not proliferate. Chanez, P., Vago, P.,Demoly, P. et al., J. Allergy Clin. Immunol. 92, 869–877 (1993).

1053. Increased numbers of hypodense alveolar macrophages in patients with bronchial asthma.Chanez, P., Bousquet, J., Couret, I. et al., Am. Rev. Respir. Dis. 144, 923–930 (1991).

1054. Mouse peritoneal macrophages: characterization of functional subsets following Percolldensity gradients. Plasman, N. and Vray, B. Res. Immunol. 14, 151–163 (1993).

1055. Separation of murine peritoneal macrophages using Percoll density gradients. Vray, B. andPlasman, N. J. Immunol. Meth. 174, 53–59 (1994).

1056. Fluorescence demonstration of cathepsin B activity in fractionated alveolar macrophages.Sakai, K., Nii, Y., Ueyama, A. et al., Cell. Molec. Biol. 37, 353–358 (1991).

1057. Effects of pulmonary surfactant and surfactant protein A on phagocytosis of fractionatedalveolar macrophages: relationship to starvation. Sakai, K., Kweon, M.N., Kohri, T. et al.,Cell. Molec. Biol. 38, 123–130 (1992).

1058. The effects of asbestos inhalation on the distribution and enhancement of immunoassociatedantigen expression of alveolar macrophage subpopulation. Inamoto, T., Georgian, M.M.,Kagan, E. et al., J. Vet. Med. Sci. 54, 829–836 (1992).

1059. Immunoglobulin E plus antigen challenge induces a novel intercrine/chemokine in mousemast cells. Kulmburg, P.A., Huber, N.E., Scheer, B.J. et al., J. Exp. Med. 176, 1773–1778 (1992).

1060. Potentiation of antigen-induced histamine release from rat peritoneal mast cells through adirect interaction between mast cells and non-mast cells. Inagaki, N., Kawasaki, H., Ueno, M.et al., Life Sci. 54, 1403–1409 (1994).

1061. Effect of phospholipase A2 inhibitor ONA-RS-082, on substance P-induced histamine releasefrom rat peritoneal mast cells. Kurosawa, M., Hisada, T. and Ishizuka, T. Int. Arch. AllergyImmunol. 97, 226–228 (1992).

1062. Eosinophil peroxidase accounts for most if not all of the peroxidase activity associated withisolated rat peritoneal mast cells. Rickard, A. and Lagunoff, D. Int. Arch. Allergy Immunol.103, 365–369 (1994).

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1063. Sensitivity in vitro of mature and immature mouse thymocytes to dexamethasone cytotoxicityand its correlation to poly ADP-ribosylation. Hoshino, J., Beckmann, G. and Kroger, H.Biochem. International 27, 105–116 (1992).

1064. Dexamethasone and etoposide induce apoptosis in rat thymocytes from different phases tothe cell cycle. Fearnhead, H.O., Chwalinski, M., Snowden, R.T. et al., Biochem. Pharmacol.48, 1073–1079 (1994).

1065. Characterization of apoptosis in thymocytes isolated from dexamethasone-treated rats.Sun, X.-M., Dinsdale, D., Snowden, R.T. et al., Biochem. Pharmacol. 44, 2131–2137 (1992).

1066. Formation of large molecular weight fragments of DNA is a key committed step to apoptosisin thymocytes. Cohen, G.M., Sun, X.-M, Fearnhead, H. et al., J. Immunol. 153, 507–516 (1994).

1067. Immunologic detection of endothelial cells in human whole blood. Sbarbati, R., de Boer, M.,Marzilli, M. et al., Blood 77, 764–769 (1991).

1068. Stimulation of rat placental lactogen-II (rPL-II) secretion by cultured trophoblasts by insulin:development of a rat placental cell culture system and effects of peptide hormones on rPL-TTsecretion in vitro. Kishi, K., Itoh, M., Kanemori, S. et al., J. Reprod. Fertil. 99, 519–527 (1993).

1069. Sedimentation for the separation of cells. Pretlow, T.G. and Pretlow, T.P. Methods: A Companionto Methods in Enzymology 2, 183–191 (1991).

1070. Nonenzymatic extraction of cells from clinical tumor material for analysis of gene expressionby twodimensional polyacrylamide gel electrophoresis. Franzen, B., Linder, S., Okuzawa, K.et al., Electrophoresis 14, 1045–1053 (1993).

1071. HL-60 cells induced to differentiate towards neutrophils subsequently die via apoptosis.Martin, S.J., Bradley, J.G. and Cotter, T.G. Clin. Exp. Immunol. 79, 448–453 (1990).

1072. Cytoplasmic transfer of the mtDNA nt 8993 T-G(ATP6) point mutation associated with theLeigh syndrome into mtDNA-less cells demonstrates cosegregation with a decrease in stateIII respiration and ADP/O ratio. Trounce, I., Neill, S: and Wallace, D.C. Proc. Natl. Acad.Sci. USA 91, 8334–8338 (1994).

1073. Morphological obervations of turkey (Meleagris galopavo) spermiophages maintained intissue culture. Perez, B.S., Derrick, Jr, F.C., Korn, N. and Thurston, R.J. Poultry Sci. 73,1597–1606 (1994).

1074. The influence of growth medium on serum sensitivity of Bacteroids species. Allan, E. andPoxton, I.R. J. Med. Microbiol. 41, 45–50 (1994).

1075. Inhibition of binding, entry, or intracellular proliferation of Ehrlichia risticii in P388D1 cellsby anti-E. risticii serum, immunoglobulin G, or Fab fragment. Messick, J.B. and Rikihisa, Y.Infect. Immun. 62, 3156–3161 (1994).

1076. Inhibition of infection of macrophages with Ehrlichia risticii by cytochalasins,monodansylcadaverine, and taxol. Rikihisa, Y., Zhang, Y. and Park. J. Infect. Immun.62, 5126–5132 (1994).

1077. Synthetic peptides analogous to the fimbrillin sequence inhibit adherence of Porphyromonasgingivalis. Lee, J.-Y., Sojar, H.T., Bedi, G.S. et al., Infect. Immun. 60, 1662–1670 (1992).

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1078. The influence of different sera on the in vitro immobilisation of Percoll purified. Treponemapallidum, Nichols strain. Engelkens, H.J.H., Kant, M., Onvlee, P.C. et al., Genitourin. Med.68, 20–25 (1992).

1079. Preliminary biochemical characterization of veil structure purified from Theileria sergenti-T. buffeliand T. orientalis-infected bovine erythrocytes. Sugimoto, C., Kawazu, S., Sato, M. et al.,Parasitol 104, 207–213 (1992).

1080. Concentration and enzyme content of in vitro-cultured Babesia bigemina-infected erythrocytes.Vega, C.A., Buening, G.M.., Rodriguez, S.D. et al., J. Protozool. 33, 514–518 (1986).

1081. Protein characterization of Babesia equia piroplasms isolated from infected horse erythrocytes.Ali, S., Sugimoto, C., Matsuda, M. et al., Parasitol. Res. 79, 639–643 (1993).

1082. Rapid transport of the acidic phosphoproteins of Plasmodium berghei and P. chabaudi fromthe intra-erythrocytic parasite to the host membrane using a miniaturized fractionation procedure.Wiser, M.F. and Lanners, H.N. Parasitol. Res. 78, 193–200 (1992).

1083. Babesia bovis: purification and concentration of merozoites and infected bovine erythrocytes.Rodriquez, S.D., Buening, G.M., Vega, C.A. et al., Exp. Parasitol. 61, 236–243 (1986).

1084. Improved method for the concentration and purification of faecal cysts of Entamoebahistolytica for use as antigen. Jyothi, Foerster, B., Hamelmann, C. et al., J. Trop. Med. andHygiene 96, 249–250 (1993).

1085. Vairimorpha necatrix: infectivity for and development in a lepidopteran cell line. Kurtti, T.J.,Munderloh, U.G. and Noda, H. J. Inv. Pathol. 55, 61–68 (1990).

1086. Purification and partial characterization of rice transitory yellowing virus. Chiu, R.-J.,Yau-Heiu, H., Chen, M.-J. et al., Am: Phytopathol. Soc. 80, 777–783 (1990).

1087. Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuoussucrose. Bustos, J., Zamora, P., Mejia, E. et al., Arch. Virol. 118, 285–288 (1991).

1088. Purification of mycoplasma-like organisms from lettuce with aster yellows disease. Jiang, Y.P.and Chen, D.A. Am. Phytopathol. Soc. 77, 949–953 (1987).

1089. Growth hormone regulates amino acid transport in human and rat liver. Pacitti, A.J., Inoue, Y.and Plumley, D.A. Ann. Surg. 216, 353–362 (1992).

1090. Oxytocin pretreatment of pregnant rat uterus inhibits Ca2+ uptake in plasma membrane andsarcoplasmic reticulum. Magocsi, M. and Penniston, J.T. Biochem. Biophys. Acta 1063,7–14 (1991).

1091. Lysophosphoinostide-specific phospholipase C in rat brain synaptic plasma membranes.Tsutsumi, T., Kobayashi, T., Ueda, H. et al., Neurochem. Res. 19, 399–406 (1994).

1092. Pharmacological characterization of inositol 1,4,5-triphosphate binding sites: relation toCa2+ release. Mouillace, B., Devilliers, G. and Jard. S. Eur. J. Pharmacol. 225, 179–193 (1992).

1093. Influence of fasting on glutamine transport in rat liver. Espat, N.J., Copeland, E.M. andSouba, W.W. J. Parenter. Enter. Nutr. 17, 493–500 (1993).

1094. Inositol 1,4,5-triphosphate binding sites copurify with the putative Ca-storage protein calreticulinin liver. Enyed, P., Szabadkai, G., Krause, K.-H. et al., Cell. Calcium 14, 485–492 (1993).

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1095. Stimulation of hepatocyte system y+-mediated L-arginine transport by an inflammatory agent.Pacitti, A.J., Copeland, E.M. and Souba, W.W. Surgery 11, 403–411 (1992).

1096. Cellular localization and characterization of proteins that bind high density lipoprotein.Hokland, B., Mendez, A.J. and Oram, J.F. J. Lipid Res. 33, 1335–1342 (1992).

1097. Dietary soybean oil changes lipolytic rate and composition of fatty acids in plasma membranesof ovine adipocytes. Jenkins, T.C, Thies, E.J. and Fotouhi, N. J. Nutr. 124, 566–570 (1994).

1098. Isolation of the plasma membrane and organelles from Chinese hamster ovary cells.Cezanne, L., Navarro, L. and Tocanne, J.-F. Biochim. Biophys. Acta 1112, 205–214 (1992).

1099. Isolation of Raja erinacea basolateral liver plasma membranes: characterization of lipidcomposition and fluidity. Smith, D.J. and Ploch, S.A. J. Exp. Zool. 258, 189–195 (1991).

1100. Structural and functional properties of plasma membranes from the filamentous fungusPenicillium chrysogenum. Hillenga, D.J., Versantvoort, H.J.M., Driessen, A.J.M. et al.,Eur. J. Biochem. 224, 581–587 (1994).

1101. Preparation of right-side-out plasma membrane vesicles from Penicillium cyclopium: a criticalassessment of markers. Ugalde, U.O., Hernandez, A., Galindo I. et al., J. Gen. Microbiol.138, 2205–2212 (1992).

1102. Demonstration of adenosine deaminase activity in human fibroblast lysosomes. Lindley, E.R.and Pisoni, R.L. Biochem. J. 290, 457–462 (1993).

1103. Specific storage of subunit c of mitochondrial ATP synthase in lysosomes of neuronal ceroidlipofuscinosis (Batten's disease). Kominami, E., Ezaki, J., Muno, D. et al., J. Biochem. 111,278–282 (1992).

1104. Effect of pH and ATP on the equilibrium density of lysosomes. Mayorga, L.S., De Veca, M.G.,Colombo, M.I. et al., J. Cell. Physiol. 156, 303–310 (1993).

1105. Cycling of an 85-kDa lysosomes membrane glycoprotein between the cell surface and lysosomesin cultured rat hepatocytes. Akasaki, K., Michihara, A., Fukuzawa, M. et al., J. Biochem.116, 670–676 (1994).

1106. Preparation of membrane vesicles from kidney cortex lysosomes using amino acid methyl ester.Harikumar, P. Biochem. Molecul. Biol. International 30, 1005–1011 (1993).

1107. Distribution and structure of the vacuolar H+ ATPase in endosomes and lysosomes fromLLC-PK1 cells. Somsel Rodman, J., Stahl, P.D. and Gluck, S. Exp. Cell Res. 192, 445–452 (1991).

1108. Rapid preparation of subsarcolemmal and interfibrillar mitochondrial subpopulation from cardiacmuscle. Chemnitius, J,-M., Manglitz, T., Kloeppel, M. et al., Int. J. Biochem. 4, 589–596 (1993).

1109. Developmental changes rat liver mitochondrial populations analyzed by flow cytometry.Lopez-Mediavilla, C., Orfao, A., San Miguel, J. et al., Exp. Cell Res. 203, 134–140 (1992).

1110. Plasmodium berghei: partial purification and characterization of the mitochondrial cytochromec oxidase. Krungkrai, J., Krungkrai, S.R. and Bhumiratana, A. Exp. Parasitol. 77,136–146 (1993).

1111. Isolation and characterization of mitochondria from turkey spermatozoa. McLean, D.J.,Korn, N., Perez, B.S. et al., J. Androl. 14, 433–438 (1993).

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1112. Isolation and characterization of gelatinase granules from human neutrophils. Kjeldsen, L.,Sengelov, H., Lollike, K. et al., Blood 83, 1640–1649 (1994).

1113. Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils. Sengelov, H., Boulay, F., Kjeldsen, L. et al., Biochem. J.299, 473–479 (1994).

1114. Subcellular localization and dynamics of Mac-1 (a[m]b[2]) in human neutrophils. Sengelov, H.,Kjeldsen, L., Diamond, M.S. et al., J. Clin. Invest. 92, 1467–1476 (1993).

1115. Subcellular localization of heparanase in human neutrophils. Matzner, Y., Vlodavsky, L.,Bar-Ner, M. et al., J. Leuk. Biol. 51, 519–524 (1992).

1116. Granules of human CD3+ large granular lymphocytes contain a macrophage regulation factor(s)that induces macrophage H2O2 production and tumoricidal activity but decreases cell surfaceIa antigen expression. Roussel, E. and Greenberg, A.H. Cell. Immunol. 134, 31–41 (1991).

1117. Properties of plasma membrane-induced amylase release from rat parotid secretory granules:effects of Ca2+ and Mg-ATP. Mizuno, M., Kameyama, Y., Yashiro, K. et al., Biochem.Biophys. Acta 1116, 104–111 (1992).

1118. Isolation of cytolytic granules from sea urchin amoebocytes. Pagliara, P. and Canicatti, C.Eur. J. Cell. Biol. 60, 179–184 (1993).

1119. Phosphatidylethanolamine synthesis by castor bean endosperm. Wang, X. and Moore, Jr, T.S.J. Biol. Chem. 266, 19981-19987 (1991).

1120. Characterization of a mitochondrial NADP-dependent isocitrate dehydrogenase in axes ofgermination sunflower seeds. Attucci, S., Rivoal, J., Brouquisse, R. et al., Plant Science 102,49–59 (1994).

1121. Microanalysis of plant mitochondrial protein synthesis products: detection of variantpolypeptides associated with cytoplasmic male sterility. Boutry, M., Faber, A.-M.,Charbonnier, M. et al., Plant Molec. Biol. 3, 445–452 (1984).

1122. Association of TMV coat protein with chloroplast membranes in virus-infected leaves.Reinero, A. and Beachy, R.N. Plant Molec. Biol. 6, 291–301 (1986).

1123. The methods for isolation of cytoplasts in several crop plants. Watanabe, M. andYamaguchi, H. Japan. J. Breed. 38, 43–52 (1988).

1124. Barley aleurone layer cell protoplasts as a transient expression system. Gopalakrishnan, B.,Sonthayanon, B., Rahmatullah, R. et al., Plant Molec. Biol. 16, 463–467 (1991).

1125. Rapid isolation of nuclei from carrot suspension culture cells using a BioNebulizer.Okpodu, C.M., Robertson, D., Boss, W.F. et al., BioTechniques 16, 154–158 (1994).

1126. Purification of highly intact plastids from various heterotrophic plant tissues: analysis ofenzymatic equipment and precursor dependency for starch biosynthesis. Neuhaus, H.E.,Batz, O., Thom, E. et al., Biochem. J. 296, 395–401 (1993).

1127. Nuclear buoyant density determination and the purification and characterization of wild-typeNeurospora nuclei using Percoll density gradients. Talbot, K.J. and Russell, P.J. Plan Physiol.70, 704–708 (1982).

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1128. Outer dense fibres of human spermatozoa: partial characterization and possible physiologicalfunctions. Henkel, R., Stale, T., Mertens, N. et al., International J. Androl. 17, 68–73 (1994).

1129. Isolation and partial characterization of the outer dense fiber proteins from human spermatozoa.Henkel, R., Stalf, T. and Miska, W. Biol. Chem. Hoppe-Seyler 373, 685–689 (1992).

1130. Endocytosis and lysosomal delivery of tissue plasminogen activator-inhibitor 1 complexes inHep G2 cells. Underhill, D.M., Owensby, D.A., Morton, P.A. et al., Blood 80, 2746–2754 (1992).

1131. Characterization of peptides bound to extracellular and intracellular HLA-DR1 molecules.Max, H., Halder, T., Kropshofer, H. et al., Human Immunol. 38, 193–200 (1993).

1132. Subcellular fractionation of cultured normal human melanocytes: new insights into therelationship of melanosomes with lysosomes and peroxisomes. Smit, N.P.M.,van Roermund, C.W.T., Aerts, H.M.F.G. et al., Biochim. Biophys. Acta 1181, 1–6 (1993).

1133. Changes in the localization of catalase during differentiation of neutrophilic granulocytes.Ballinger, C.A., Chamindrani Mendis-Handagama, S.M.L., Kalmar, J.R. et al., Blood 83,2654–2668 (1994).

1134. Biosynthesis and processing of cathepsin G and neutrophil elastase in the leukemic myeloidcell line U-937. Lindmark, A., Persson, A.-M. and Olsson, I. Blood 76, 2374–2380 (1990).

1135. Spheroplast formation and partial purificaiton of microbodies from hydrocarbon-grown cellsof Cladosporium resinae J. Carson, D.B. and Cooney, J.J. Ind. Microbiol. 3, 111–117 (1988).

1136. Fractionation of carcinoembryonic antigen and related antigens in normal adult feces usinga gradient medium of Percoll. Kuroki, M., Kuwahara, M., Murakami, M. et al., Prep. Biochem.24, 25–40 (1994).

1137. Separation of inner and outer membrane vesicles from Escherichia coli in self-generatingPercoll gradients. Morein, S., Henricson, D. and Rilfors, L. Anal. Biochem. 216, 47–51 (1994).

1138. Protein content and cAMP-dependent phosphorylation of fractionated white perch retina.McMahon, D.G., Rischert, J.C. and Dowling, J.E. Brain Res. 659, 110–116 (1994).

1139. C-type natriuretic peptide increases cyclic GMP in rat cerebral microvessels in primary culture.Kobayashi, H., Ueno, S., Tsutsui, M. et al., Brain Res. 648, 324–326 (1994).

1140. Putrescine and paraquat uptake in human lung slices and isolated type II pneumocytes.Hoet, P.H.M., Lewis, C.P.L., Demedts, M. et al., Biochem. Pharmacol. 48, 517–524 (1994).

1141. Separation and behavior in vitro of hemocytes from the moth. Pseudoplusia includens.Pech, L.L, Trudeau, D. and Strand, M.R. Cell Tissue Res. 277, 159–167 (1994).

1142. Morphological and functional heterogeneity of frog melanotrope cells. de Aguilar, J.L.,Tonon, M.C., Ruiz-Navarro, A. et al., Neuroendocrinology 59, 176–182 (1994).

1143. Hormonal storage patterns and morphological heterogeneity of porcine gonadotrope cellsduring postnatal development. Torronteras, R., Castaño, J.P., Almaden, Y. et al., Molec.Cell. Endocrinol. 97, 51–59 (1993).

1144. Normal rat uterine stromal cells in continuous culture: characterization and progestin regulationof growth. Cohen, H., Pageaux, J.-F., Melinand, C. et al., Eur. J. Cell Biology. 61, 116–125 (1993).

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1145. Characteristics of endothelin receptors and intracellular signaling in porcine inner medullarycollecting duct cells. Migas, I., Bäcker, A., Meyer-Lehnert, H. et al., Amer. J. Hypertension6, 611–618 (1993).

1146. Metabolism of arachidonic acid by guinea pig clara cells. Laporte, J., Hallee, A., Maghni, K.et al., Prostaglandins 41, 263–281 (1991).

1147. Cortical alveoli of Paramecium: a vast submembranous calcium storage compartment.Stelly, N., Mauger, J.-P., Claret, M. et al., J. Cell. Biology 113, 103–112 (1991).

1148. Morphological, biochemical, and functional characterization of bulk isolated glial progenitorcells. Lubetzki, C., Goujet-Zale, C., Gansmüller, A. et al., J. Neurochem. 56, 671–680 (1991).

1149. Density fluctuation during the cell cycle in the defective vacuolar morphology mutants ofSaccharomyces cerevisiae. Ohsumi, M., Uchiyama, K. and Ohsumi, Y. J. Bacteriol. 175,5714–5716 (1993).

1150. Toward cord blood banking: density-separation and cryopreservation of cord blood progenitors.Newton, I., Charbord, P., Schaal, J.P. et al., Exper. Hematology 21, 671–674 (1993).

1151. Isolated brush cells of the rat stomach retain their structural polarity. Luciano, L.,Armbruckner, L., Sewing, K.-F. et al., Cell Tissue Res. 271, 47–57 (1993).

1152. The concentration of estrogen receptors in rabbit uterine myocytes decreases in culture.Sadovsky, Y., Riemer, K. and Roberts, J.M. Amer. J. Obstet. Gyn. 167, 1631–1635 (1992).

1153. The major glycolipid recognized by SP-D in surfactant is phosphatidylinositol. Persson, A.V.,Gibbons, B.J., Shoemaker, J.D. et al., Biochemistry 31, 12183–12189 (1992).

1154. Micronuclear DNA from Paramecium tetraurelia: serotype 51 A gene has internally eliminatedsequences. Preer, L.B., Hamilton, G. and Preer, Jr., J. J. Protozool. 39, 678–682 (1992).

1155. Purification of Pneumocytis carinii trophozoites and identification of their circulating antigens.Ishimaru, T., Shimono, N., Sawae, Y. et al., J. Clin. Microbiol. 30, 3263–3267 (1992).

1156. Regulation if chorionic gonadotropin-a and chorionic somatomammotropin messengerribonucleic acid expression by 8-bromo-adenosine 3' ,5'-monophosphate and dexamethasonein cultured rhesus monkey syncytiotrophoblasts. Golos, T.G., Handrow, R.R., Durning, M. et al.,Endocrinology 131, 89–100 (1992).

1157. Cryptosporidium merozoite isolation and purification using differential centrifugation techniques.Regan, S., Cama, C. and Sterling, C.R. J. Protozool. 38, 202s–204s (1991).

1158. Human stools as a source of viable colonic epithelial cells. Iyengar, V., Albaugh, G.P.,Lohani, A. et al., FASEB J. 5, 2856–2859 (1991).

1159. An autoregulatory process for androgen production in rat thecal-interstitial cells. Simone, D.A.and Mahesh, V.B. Biol. Reproduc. 48, 46–56 (1993).

1160. Effects of aging on the human ovary: the secretion of immunoreactive a-inhibin and progesterone.Pellicer, A., Simón, C., Marí, M. et al., Fertil. Steril. 61, 663–668 (1994).

1161. Functional and ultrastructural characteristics of two types of rat granulosa cell cultured inthe presence of FSH or transforming growth factor a (TGF-a). Sanbuissbo, A., Lee, G.Y.and Anderson, E. J. Reprod. Fertil. 98, 367–376 (1993).

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1162. The influence of acetazolamide and amlodipine on the intracellular sodium content of ratproximal tubular cells. Wong, P.S.K., Barclay, P.L., Newman, M.J. et al., British J. Pharmacol.112, 881–886 (1994).

1163. Cellular and subcellular localization of enzymes of arginine metabolism in rat kidney.Dhanakoti, S.N., Brosnan, M.E., Herzberg, G.R. et al., Biochem. J. 282, 369–375 (1992).

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Author index

AAalto, M. 34Abrahamsohn, I.A. 400Abuzeid, M.I. 1021Adamski, J. 772Advani, S. 664Aerts, H.M.F.G. 1132Agrapart, M. 390Ahlberg, J. 132, 145Ahmad, M. 737Aigrain, Y. 192Ainsworth, A.J. 850Aitken, J. 1017Aitken, R.J. 859, 1013, 1014, 1016Akasaki, K. 1105Akazawa, T. 88Akerlof, E. 662, 857Akin, D.T. 727Akiyama, K. 972Alberti, S. 196Albaugh, G.P. 1158Alderman, E.M. 114Aldred, L.F. 353Aleotti, A. 196Aley, S.B. 486Ali, F.M.K. 261Ali, S. 1081Ali, S.Y. 821Allam, S. 71Allan, E. 1074Allen, P.M. 464Allen, S.L. 401Almaden, Y. 1143Almagor, M. 1081Alsina, J. 592Altevogt, P. 93, 225Ampel, N.M. 919Andersen, C.Y. 1004Anderson, E. 1161Anderson, J.O. 185Andersson, J. 443Andrighetto, G. 397, 399Andrysiak, P.M. 623Antonaci, S. 336Aoshima, H. 189Appelkvist, E.L. 223Ar, D. 170Arai, K. 873Arai, S. 465Arcidiacono, A. 466, 745

Armbruckner, L. 1151Arnould, T. 955Arock, M. 968Arrowood, M.J. 705Asano, T. 306Ashman, R.F. 427Athlin, L. 894Atkinson, K. 520Attucci, S. 1120Auersperg, N. 845Auger, J. 1023Augery, Y.L. 479Augustin, A.A. 144Austen, K.F. 304Austin, F.E. 825Avraham, H. 1027Avron, B. 467Awano, M. 1025Axberg, I. 307

BBabiuk, L.A. 783, 867, 915Bach, D. 304Bachere, E. 686Bacon, S. 944Bae, Y.J. 729Baenziger, J.U. 35Bainton, D.F. 961Bakac, T. 491Baker-Zander, S.A. 321Bako, G. 488Balbo, P. 499Baldi, L. 892, 896Baldwin, R.W. 266Baldwin, W.W. 493Ball, E.D. 262Ballet, J.-J. 390Ballinger, C.A. 1133Bandeira, A. 501Banwart, G.J. 402Bar-Ner, M. 1115Barclay, P.L. 1162Bard, J. 714Barker, J.E. 944Barlozzari, T. 468Barnett, L. 926, 1032Barnwell, J.W. 263, 551Baron, S. 545Barros, C. 1003Barthelemy, C. 1022

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Bartlett, S. 135Bartolf, M. 58Bartschi, H. 182Bartus, H.F. 368Basel, R.M. 402Basner, R. 68Basu, S. 355Batt, R.M. 403Batteiger, B. 434Batz, O. 1126Bayer, B. 108Beachy, R.N. 1122Beagley, K.W. 913Beaty, P.S. 688Beaumont, T.J. 329Becich, M.J. 308Beckmann, G. 1063Bedi, G.S. 1077Beer, N. 981Beisker, W. 943Bejarano, G.C. 919Bekesi, J.G 395Belkner, J. 784Bell, C.M. 525Bell, L.V. 585Bell, P.A. 211Bellemare, G. 663Beller, D.I. 464Bellgrau, D. 307Belmont, J.W. 1044Belsham, G.J. 110Bennedsen, J. 505Bennett, G.D. 264Berends, C. 958Berg, P.A. 198Bergada, C. 988Bergeron, J.J.M. 318Bergström, R. 797Berle, E.J. 356Bernabe, R.R. 41Bernard, G.W. 38Bernier, I. 326Bernstein, R.E. 334Berridge, M.V. 303Berrih, S. 48Berry, M.N. 607Bertani, A. 924Bertini, F. 618Berzins, K. 410Bes, J.-C. 291Beswick, T.M. 187Betsche, T. 654Bettaieb, A. 1029

Betz, A.L. 37, 170, 309Bhalla, V.K. 674Bhumiratana, A. 1110Bianchi de di Risio, C. 700Biemond, I. 174Bienzle, U. 179Binnerts, J.S. 260Bird, C.E. 325Biron, C.A. 404Bischoff, S. 973Bishop, N.H. 481Bismuth, G. 1028Bitensky, M.W. 259Biundo, T. 316Bjerke, T. 970Bjursten, L.M. 635Björntorp, P. 6Blaese, R.M. 149Blanchard, D.K. 904Blanchard, Y. 876Blaxhall, P.C. 559, 624Bligny, R. 324Bloem, J. 566Blom, M. 964Bloom, A.L. 40Bloom, E.T. 169, 358Blume, H. 86Blyznak, N. 228Bocchieri, M.H. 224Bodmer, J.G. 278Boettiger, D. 552Bogoch, E 1031Boketoft, A. 531Bollengier, F. 882Bolscher, B.G.J.M. 922, 929Bonavida, B. 359, 539Bonfigli, A.R. 947Bongso, A. 1002Boni, U. 370Boning, D. 945Bonnard, G.D. 302, 457, 458Bonomo, L. 336Bonven, B. 233Boogaerts, M.A. 611Booth, R. 18, 146, 199Boraschi, D. 196, 257, 433Bord, M.J. 656Borisova, E.A. 659, 690Born, W. 36, 265Borrebaek, B. 444Borremans, B. 1038Borth, W. 439Bos, L. 250

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Bosch, F.H. 938Boss, W.F. 1125Bosslet, K. 225Botta, G. 606, 683Bottero, L. 511Bottomly, K. 115Boucher, L. 939Boudouard, M. 770Boulay, F. 1113Boumendil-Podevin, E.F. 469Bourget, G. 939Bourhis, J.H. 1032Bousquet, J. 1053Boutry, M. 1121Bowers, B. 823Bowman, P.D. 37, 170, 309Boye, N.P. 811Braathen, L.R. 357Bracha, R. 467Bradbury, J.M. 214Bradford, M.M. 90Bradley, J.G. 1071Bradley, T.P. 359Braet, F. 977Braide, M. 635Branch, D.R. 470Brandslund, I. 226, 352Brattig, N.W. 747Braun, J. 464Bridges, K.R. 387Briones-Urbina, R. 488Britvan, L.J. 372Brock, W.A. 50, 335, 381Brockbank, K.J. 116Brooks, C.G. 266Brosnan, M.E. 1163Brouquisse, R. 1120Brovelli, A. 763Browning, J.Y. 171Broyles, R.H. 128, 349Brunk, U. 223Brunstedt, J. 118Bruynzeel, P.L.B. 594Brysk, M.M. 172, 715Brüggen, J. 39Bucci, L.R. 335Buckinham, D. 1013, 1014, 1016, 1017Budenz, R.W. 38Budzko, D. 830Buening, G.M. 1080, 1083Buhrer, C. 891Buitrago, A. 5Burger, M. 78

Burgers, J.A. 963Burgess, A.W. 70, 119Burgun, C. 558Burmester, G.R. 471Burrichter, H. 419Burstein, S.A. 868Bustos, J. 1087Buurman, W.A. 360Bwayo, J.J. 371Bygrave, F.L. 327, 532Bäcker, A. 1145Böck, G. 346

CCaime, L. 871Caine, Y. 612Calabrese, D.W. 1042Calaminus, J.M. 39Calhoun, W.J. 1048, 1050Callard, R.E. 127, 173, 331, 718Cama, V. 1157Cambier, PÅ.H. 174Camerion, M. 317Cameron-Mills, V. 227Campana, A. 466Campos, M. 915Candolfi, E. 855Canicatti, C. 1118Canning, E.U. 828Canonica, G.W. 175, 338Cantell, K. 162, 682Cantrell, D.A. 266Cao, J. 943Capley, G. 850Capra, J.D. 435Caprari, P. 350Capron, A. 364Capucci, A. 927Car, B.D. 1035Caraux, J. 254Carde, J.-P. 324Carlson, F. 470Carlsson, B. 46Carlsson, R. 531Carpen, O. 472, 541Carrasco, L. 496Carrascosa, A.L. 570Carraway, K.L. 120, 191Carson, D.B. 1135Carsten, A.L. 236Carty, S.E. 121, 267Cash, D.J. 189Castaño, J.P. 1143

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Castellano, M. 927Castoldi, G. 814Caulfield, J.P. 581Cawley, J.C. 173Cerasoli, Jr., F. 807Cerra, Jr., F. 120Cezanne, L. 1098Chakraborty, S. 874Chakravarthy, B.R. 596, 610Chamberline, K.G. 791Chamindrani Mendis-Handagama, S.M.L. 990, 1133Chan, P.J. 1006Chan, S.Y.W. 853, 1007, 1009Chan, W.-S. 655Chan, Y.M. 1009Chandler, J.E. 1024Chandoga, I. 361Chanez, P. 1052, 1053Channing, C.P. 418Chap, H.J. 54, 291Chapman, J. 1001Charbonnier, M. 1121Charbord, P. 1150Chartrand, C. 826Chatelain, C. 820Chaudhuri, S. 355Check, J.H. 1088Chemes, H. 988Chemnitius, J.-M. 1108Chen, M.-J. 1086Chen, T.A. 1088Chen, Y. 1001Cheng, C.Y. 999Cheng, L. 255Cheng, Y.-S. E. 405Cherry, B. 1041Chestnut, W. 416Chesterton, C.J. 187Chestier, N. 937Chi, D.S. 288Childs, W.C. 767Chiu, R.-J. 1086Chowdhury, N.R. 985Christ, H. 15Chu, E. 337Chu, F.W. 637Chua, B.H.L. 123Chua, N.-H. 76, 135Chukhlovin, A.B. 659Chwalinski, M. 1064Cianciolo, G.J. 268Ciani, C.M.L. 581

Cigorraga, S. 988Claret, M. 1147Clark, A.F. 325Clarke, B.J. 823Clarkson, A.F. 13Cochrum, K. 124Cohen, A. 901Cohen, G.M. 1066Cohen, H. 1144Colas, B. 125Colby-Germinario, S. 284Colditz, I.G. 578Collard, M.W. 993Colley, D.G. 344Collins, W.E. 623Colombo, M.I. 618, 1104Connor, J. 942Conway, E.M. 957Cook, H.W. 806Cook, J.S. 625Cooke, B.A. 353, 176, 310, 994Cooney, J.J. 1135Cooper, A.J. 126Cooper, M.D. 376, 420Copeland, E.M. 1093, 1095Corcione, A. 892, 896Cornelissen, A.W.C.A. 269Correa, R. 945Cotter, T.G. 1071Couret, I. 1053Courtoy, R. 8Cousineau, J. 362Coutinho, A. 144Covault, J. 832Cowley, s. 1027Craig, J. 160Craig, S. 967Cranna, C.E.G. 146Creek, K.E. 406Cronkite, E.P. 236Cruise, K.M. 100, 119Cryer, A. 831Cudd, A. 644Cuneo, A. 814Curman, B. 7

DD´Agata, R. 171Dabrowski, M. 818Dagg, M.K. 299Dahinden, C.A.. 973Dalet, C. 270Dallaire, L. 362

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Dallner, G. 223Damiano, A. 609Danielsson, C. 931Danneus, A. 93Darai, G. 138Darnell, B. 545Dartt, D. 134Daubert, J.H. 473, 484Davey, F.R. 184, 540David, J.R. 279Davies, D.E. 836Davies, M.D.J. 271Davis, J.P. 634Day, D.A. 651De Aguilar, J.L. 1142De Angelis, V. 951De Bast, M. 820De Boer, D. 768De Boer, M. 922, 929, 1067De Klein, A. 922De Kretser, D.M. 990De Kretser, T.A. 278, 879, 889De Lamirande, E. 673, 817De Landazuri, M.O. 243De Matteis, M.C. 164De Monchy, J.G.R. 958De Simone, C. 297, 475De Sousa, G. 975De Veca, M.G. 1104De Vries, K. 417De Weger, R.A. 177, 460De Zanger, R. 977Debeer, L.J. 455, 474DeBernardo, S.L. 84Debey, P. 270DeBoni, U. 480, 856Debout, C. 533Decker, J.M. 149DeFranco, A.L. 704Degenne, D. 590Degos, L. 1028Dejbakhsh-Jones, S. 1033, 1045Delgado-Escueta, A. 522, 786Delque, P. 369Delree, P. 794Delrez, M. 8Demedts, M. 1140Demoly, P. 1052Dempsey, R.A. 363Den Otter, W. 177Dench, J.E. 12, 106DeNicola, D.B. 909Denis, M. 952

Dennenm, G.J. 250Denton, R.M. 110Denucé, J.M. 168Dereszewski, G. 653Derrick, Jr., F.C. 1073Desaymard, C. 141Desplaces, A. 254Deutsch, M.R. 467Deutscher, S.L. 406Devauchelle, N. 669Devenny, J.J. 259Devilliers, G. 1092Dhanakoti, S.N. 1163Dhaunsi, G.S. 703Di Cola, D. 946Diamond, M.S. 484, 1114Diaz-Espada, F. 314Dickinson, A.M. 476Diener, B. 958Dimitriu-Bona, A. 471Dinarello, C.A. 363Dinauer, M.C. 929Dinsdale, D. 1065Dirami, G. 994Dissous, C. 364Ditterbrandt, M. 85Dix, C.J. 176, 310Djerassi, C. 835Dock, N.L. 184Dohlstein, M. 531Dolken, G. 1030Domsch, C. 365Donelli, G. 297, 475Dooley, D.C. 311Dooley, R.K. 912Dorange, G. 669Dorn, A.R. 128, 349Dou, M. 975Douce, R. 322, 324, 415Douste-Blazy, L. 54, 291Dowling, J.E. 1138Downs, E.C. 928Downs, J.T. 137Doyle, A.T. 393Doyle, D. 219Driessen, A.J.M. 1100Dufer, J. 254Dugas, B. 902Dullens, H.F.J. 177Dulski, P. 752Dunkley, P.R. 641, 754, 870Dunn, F.E. 411Durban, E. 552

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Durnam, D.M. 916Durning, M. 1156Duwe, A. 329Dvorak, A.M. 477, 536, 586Dvorak, H.F. 536Dwek, R.D. 129

EEbert, E.C. 897Eckels, D.D. 340Ecknauer, R. 178Edery, M. 652Edwards, B. 235Ehlin-Henriksson, B. 538Einarsson, M. 26Eisinger, M. 380Ekerdt, R. 133, 232Ekert, H. 661Elliott, T. 442Ellis, W.M. 527, 661, 675Ellory, J.C. 407, 461Emerson, D.M. 216Enerbäck, L. 9Engel, W.D. 108Engelkens, H.J.H. 1078Engers, H. 51England, P.J. 116Enyed, P. 1094Epping, R.J. 532Epstein, O. 87Eriksson, S. 408Ernst, M. 529Espat, N.J. 1093Espinal, C.T. 440Estable, M.C. 663Esteve, C. 254Evans, J. 87Evans, L. 821Evatt, B.L. 1036Ezaki, J. 1103

FFaber, A.-M. 1121Fagan, G. 124Fahey, J.J. 251Falcioni, G. 947Fanger, M.W. 262Farram, E. 130Fast, L.D. 497Faure, C. 669Fauvel, J. 627Favreau, C. 142

Fawzy, F. 903Fearnhead, H.O. 1064, 1066Fehlmann, M. 270Fehniger, T.E. 427Fehr, J. 584, 760, 966Feige, U. 230, 366Feldman, B.F. 678Feldmeier, H. 170Felici, M. 367Fernandez, C. 628Ferroni, L. 934Feucht, H.E. 99, 131, 245Fiete, D. 354Fincato, G. 925Findlay, K. 1000Fink, U. 44, 192Fiorilli, A. 792Firmenich, A.A. 668Fischer, S.M. 312Fischer, T.J. 614Fisker, D. 226Fitzgerald, L.M. 863Fitzsimons, P.J. 409Fizzotti, M. 951Flad, H.-D. 69, 453, 636Flannery, G.R. 266Flasch, M.V. 674Flatmark, T. 89Fleischer, M. 32Fluks, A.J. 180Flurkey, K. 911Foegh, M. 495Foerster, B. 1084Ford, H.C. 303Ford, W.C.L. 1005Forssén, M. 332Forsum, U. 10, 241, 373Forsyth, J.R.L. 696Forti, G. 1051Fortin, J.-A. 182Fortunato, A. 94Fotouhi, N. 1097Frache, P. 855Frank, R. 99, 131Frankenburg, S. 544Franks, R., 342Fransen, J.A.M. 959Franzen, B. 1070Fraser, J. 408Freedman, A.S. 900Frentzen, M. 478Fresno, M. 496Frickhofen, N. 272

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Fricot, G. 1022Friis, J. 233Fuare, C. 669Fuchs, H.J. 780Fuchshuber, P.R. 907Fudenberg, H.H. 114Fujihashi, K. 913Fujiwake, H. 181Fukai, K. 849Fukuchi, K. 895Fukuoka, S.-I. 619Fukuzawa, M. 1105Fumarola, E. 336Furlan, V. 182Furlanetto, M.P. 1039

GGabriel, J. 410Gad, M.Z. 976Gadd, G.M. 524Gage, S. 860Gagnon, C. 673, 817Gahl, W.A. 787Gaillard, M.L. 959Gal, S. 597Gale, J.S. 303, 313Galembeck, F. 571Galgiani, J.N. 919Galindo, I. 1101Galkowski, H. 803Galla, H.J. 803Gallart, T. 793Gallin, J.I. 573Ganczakowski, M. 514Gandour, M. 30Ganf, G. 201Gansmüller, A. 1148Gao, X. 953Garbrecht, M. 30Garcia-Rozas, C. 314Garin, M.I. 937Garner, D.L. 423Garvey, J.S. 20Gasnier, F. 713Gasser, K.W. 775, 840Gasser, S.M. 677Gebhart, W. 346Gehan, R.S. 337Genevier, H. 1034Gennaro, R. 148Genot, E. 1028George, J.N. 684Georgian, M.M. 1058

Georgiou, G.M. 527, 666, 675Gerber, H.A. 428Ghatak, S. 941Gibbons, B.J. 1153Giddings, J.C. 40Giesa, F.R. 368Gieselmann, V. 68, 71Gillespie, E. 347Ginsburg, H. 740Ginzton, N. 1033Gitler, C. 561Giudicelli, J. 369Giuliani, A. 934Glaser, D. 788Gluck, S. 1107Glaumann, H. 132, 145Gmelig-Meyling, F. 101, 183Godberg, J. 184, 212Goforth, D.P. 998Golay, J. 927Gold, B. 448Goldenberg, S.S.S. 370, 856Goldenhersch, M.A. 273Golding, M. 310Goldstein, A.H. 185Goldstein, G.W. 37, 170, 309Goldwasser, E. 286, 386Golightly, M.G. 530Golos, T.G. 1156Good, R.A. 273Goodman, D.B.P. 234Gopalakrishnan, B. 1124Gorman, R.M. 702Gorssman, N. 129Gortjan, Jr., H.E. 171Goujet-Zale, C. 1148Goust, J.-M. 62Grab, D.J. 371, 756Grabowski, S.M. 379Graetzer, R. 493Graham, J.M. 62Granger, S. 342Gratzl, M. 133, 134, 232, 722Gray, J.D. 266Graziano, F.M. 548Greaser, M.L. 147Greenberg, A.H. 1116Greer, J. 944Grelloni, F. 947Gresham, G. 861Grey, H.M. 416Griebel, P.J. 783Griffiths, A.M. 193

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Grimelius, L. 1, 2, 3, 35, 107Grimm, E.A. 429Grinstein, S. 216Grivell, A.R. 607Groenwegen, G. 360Gross, A. 544Gross, M. 694Grosshans, E. 15Grossman, A. 135Groth, D.F. 143Grunberger, T. 901Grzych, J.-M. 364Grönvik, K.O. 16, 443Gubish, E.R. 321Guerin, J.F. 526, 1019Guerrero, R. 642, 646Guillard, R.R. 28, 60Gulløv, K. 233Gustin, M.C. 234Gutierrez, C. 41Guzman, R. 222Gylfe, E. 5Gärtner, I. 97, 98Götz, U. 389

HHaase, W. 111Hadam, M.R. 99, 131, 245Haff, L.A. 42Haffner, H. 556Hahn, C. 832Hakos, G. 681Halder, T. 1131Hall, D.O. 12, 106Hall, M. 315Hall, R.E. 550Hallee, A. 1146Halliwell, B. 12Hamamah, s. 1022Hamburger, A.W. 411, 574Hamelmann,, C. 1084Hamilton, A.J. 828Hamilton, G. 1154Hammarstrom, M.-L. 894Hammel, I. 586Hanbauer, I. 887Handa, K. 412, 516Handagama, P.J. 676, 678Handrow, R.R. 1156Hanes, D. 124Hanff, P.A. 481, 593Hansen, C.T. 328Hanson, D.F. 136

Hapel, A.J. 815Harada, H. 869Harbeck, R.J. 316Hardin, J.A. 137Hardy, M. 204Haregewoin, A. 482Harford, J.B. 387Harikumar, P. 1106Harkiss, D, 1013, 1017Harms, C.T. 165Harms, E. 138Harrision, S.M. 753Harrison, F.L, 187Hartzman, R.J. 340Haston, W.S. 508, 537Hasumura, S. 485Hatten, M.E. 598Hattori, T. 483Hawajiri, H. 619Hayaishi, O. 587Hayakawa, T. 972Hedger, M.P. 743, 804, 879Heidrich, H-G. 293Heimpel, H. 272Heinz, E. 478Heit, W, 272Heldin, P. 978Helfand, S.L. 329Helgestad, J. 970Heller, G. 926Heller, W. 813Hellstrand, K. 899Helwig, M. 685Henderson, D.C. 163Henderson, W.R. 139Hendil, K.B. 188Henkel, R. 1128, 1129Henne, C. 905Henri, A. 1029Henricson, D. 1137Henriksson, C. 5, 102Herberle-Bors, E. 117Herberman, R.B. 243, 252, 328, 333, 351Herd, T. 94Hermodsson, S. 899Hermse, A. 250Hernandez, A. 1101Herzberg, G.R. 1163Hess, G.P. 189Hester, R.B. 274Heuberger, B. 389Heyman, B. 397, 399Hibbin, J.A. 667

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Higgins, M. 788Hildebrandt, A. 621, 842Hillenga, D.J. 1100Hilliard, J.K. 207Hilz, H. 31, 105, 166Himmel, H.M. 813Hirata, F. 483Hirayama, M. 413Hirtenstein, M. 27, 55, 59Hisada, T. 1061Hiserodt, J.C. 372Hjelm, E. 373Hjorth, R. 190, 275Ho, J. 1002Hock, B. 294Hoenderboom, J.M. 269Hoet, P.H.M. 1140Hoffman, A.A. 316Hoffman, T. 483Hofland, L.J. 816, 886Hogarth, P.M. 100Hohman, T.C. 823Hokland, B. 13, 1096Holda, J.H. 276, 1043Holden, H.T. 328Holian, A. 473, 484Holland, J.F. 395Holler, E. 908Holley, M.C. 800Holm, B. 1045Holstein, A.F. 31Holt, P.G. 734Homma, S. 485Hooke, R.E. 858, 930Hopfer, U. 840Hori, R. 237Hoshino, J. 1063Hosking, C.S. 500Hotzl, C. 908Houghton, A.N. 380Howard, R.J. 263, 486Howell, S.L. 315Hrubec, T.C. 724Huang, A.H.C. 378Hubbard, H.L. 459Huber, N.E. 1059Huberman, H.S. 323Huff, T.F. 487Huggins, J.W. 120, 191Hull, M.G.R. 1005Hultenby, K. 983Hultström, D. 332, 518Humphries, M. 235

Hutchins, D. 11Håkansson, S. 810Häyry, P. 61, 67, 153, 203Höpfel, C. 424Höök, M. 24, 29

IIhalamulla, R.L. 854Iizuka, R. 4, 744Ikeda, H. 414Imagawa, W. 222Inaba, K. 608, 819Inaba, M. 1040Inagaki, N. 1060Inamoto, T. 1058Ingerslev, J. 808Inglés, J. 793Inoue, H. 1046Inoue, T. 236Inoue, Y. 1089Inui, K.-I. 237Irons, R.D. 543Ishibashi, T. 626, 868Ishida, H. 936Ishikawa, A. 414Ishikawa, H. 374Ishimaru, T. 1155Ishiyama, T. 895Ishizuka, T. 1061Islam, M.N. 488Issekutz. T. 337Itoh, M. 465, 516Itoh, M. 785, 1068Ivey, K.J. 809Iwai, K. 181Iyengar, V. 1158

JJackson, A. 655Jackson, C. 12, 43, 106, 241, 338Jacobs, A. 261, 534Jacobs, P.C. 1037Jaffe, C.L. 489Jagannadha, R.A. 996Jain, N.C. 676Janeway, C.A. 115Janossy, G. 342Jard, S. 1092Jarina, A.K. 1002Jarvie, P.E. 641Jeansson, J.S. 156Jenkins, T.C. 1097

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Jenkins, W.J. 13, 87Jepsen, L.V. 238Jiang, Y.P. 1088Jirillo, E. 336Jockusch, B.M. 298Johansson, H. 1, 2, 3, 35Johnson, G. 204Johnson, J. 245Johnson, L.R. 178Johnson, R.G. 121, 267Johnsson, A. 57Johnston, J.M. 1051Joiner, C.H. 935Jollés, P. 326Jones, B.M. 534Jones, R.B. 434Jones, S.E.M. 462Jong, E.C. 139Jonsson, A. -K. 190Jonsson, A. 107Jordan, T.W. 303Joseph, S.K. 604Jost, C.R. 959Jouannet, P. 1023Journet, E.-P. 324, 415Jouvenaz, D.P. 239Joy, K.W. 109Julius, M.H. 345, 506Julkunen, I. 643Jung, A. 108, 293Jurd, R.D. 74Jurecic, R. 1044Jyothi, R. 1084Järveläinen, H. 203

KKabelitz, D. 44, 175, 192Kagan, E. 1058Kakinuma, K. 305Kakiuchi, T. 416Kalmar, J.R. 1133Kamata, Y. 759Kameyama, Y. 1117Kan, S.C. 320Kanamori, S. 1068Kaneda, M. 638Kaneko, S. 25, 648, 660, 679Kant, M, 1078Kaplan, L. 26Karlik, S.J. 599Karlsson, M. 6Kartenbeck, J. 138Kasugai, T. 974

Katsoff, D. 1008Katz, P. 375Katzin, A.M. 400Kauffman, F.C. 979Kauffman, H.F. 417, 812Kawaguchi, A. 1025Kawaguchi, S. 563Kawasaki, H. 1060Kawata, T. 785Kawazu, S. 1079Kay, H.D. 490Kay, M.M. B. 140, 264Keil, G.M. 507Kell, D.B. 193Keller, S.E. 317Kelley, J.E.T. 236Kelley-Card, L. 874Kelsey, R.G. 865Kennard, W.C. 843Kent, C. 844Kepley, C. 967Kerr, J.B. 568, 658Keystone, E. 1031Khalfoun, B. 590Khan, M.N. 240, 318Khan, R.J. 240, 318Khan, S.A. 986Khare, S. 941Kien, T. 855Kientsch, R. 108Kilani, R.T. 877Killick, K.A. 194, 339Kimber, I. 491, 492Kimura, A.K. 16, 83, 228Kimura, I. 969Kindl, H. 49Kinnear, A.J. 201Kinoshita, T. 725Kinsky, R. 150Kishi, K. 1068Kishino, Y. 834Kiss, A. 418Kiss, T. 738Kitazawa, S. 237Kittler, M. 166Kjeldsen, L. 961, 1112, 1113, 1114Kjellén, L. 23, 553Klareskog, L. 10, 241, 338, 373Klebanoff, S.J. 139Kleene, K.C. 448Klein, G. 538Klein, J.R. 910Kling, O.R. 847

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LLacarelle, B. 975Laemmli, U.K. 280, 677LaFond, R.E. 721Lagunoff, D. 92, 1062Laharrague, P. 633Lahteenmaki, P. 377Lamaziere, J.-M.D. 595Lamb, J.R. 340Lamberts, S.W.J. 816, 886Lance, C. 852Landemore, G. 533Landry, D. 826Lange, A. 636Lange, W. 1030Langmoore, J.P. 401Langreth, S.G. 447Lanneau, M. 670Lanners, H.N. 1082Lanz, O. 558Lanzavecchia, A. 421Laporte, J. 1146Laroche, L. 48Lasky, L.C. 671

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47, 81, 228, 289, 408Lavoie, A. 620Lavoie, J.C. 630Lawrence, D.A. 914Lawrence, W.A. 733Laws, A.O. 649Lawson, M.P. 835Layton, J.E. 796Lazdins, J.K. 279Le Goff, L. 968Le Lannou, D. 876Leal-Pinto, E. 746Lee, C.S. 251Lee, G.Y. 1161Lee, J.H. 375Lee, J.-Y. 1077Lee, S.R. 591, 831Leech, J.H. 551Lees, P. 858, 930Lehto, V.-P. 472, 541Leibnitz, R.R. 909Leimgruber, W. 84Leithauser, F. 905Lejeune, H. 989Lemkin, P.F. 486Lenchitz, B. 189Leonce, S. 1023Leone, V.M. 897Lessley, B.A. 423Leto, K.L. 564Leung, K.H. 341Levene, R.B. 595Levering, P.R. 417Levitt, D. 229Lewis, C.D. 280Lewis, C.P.L 1140Li, P.S. 991Liang, C.T. 255Liang, Z. 378, 613Liau, S. 898Liberati, A.M. 951Lichtenberg, V. 105Lichtenstein, A. 575Lichtenstein, L.M. 347Liel, Y. 920Lif, I. 91Lin, T. 992Linch, D. 173Lindahl, U. 17Lindblom, J.B. 16, 93Lindena, S. 1070

Linder, S. 1070Lindley, E.R. 1102Lindmark, A. 1134Lindsey, J.A 928Lindström, P. 883Linenberger, M.L. 1047Lins, J.A. 123Lipham, W. 548Lisak, R.P. 204, 244, 413Liu, W.K. 802Livett, B.G. 284Ljunghall, S. 107Llanos, M. 1010Lloyd, G.C. 459Lloyd, J.B. 836Lo, S.J. 379Lobo, R. 377Lohani, A. 1158Lohmeyer, J. 245Loir, M. 670, 838Lollike, K. 1112Londner, M.V. 151, 544London, J. 48Longtin, J.L. 50, 381Lopez-Botet, M. 243Lopez-Mediavilla, C. 1109Lotzova´, E. 907Louache, F. 1029Louis, J. 51, 482Lovenberg, W. 774Lovins, R.E. 114Lubetzki, C. 1148Luciano, L. 1151Lucivero, G. 336Luig, A. 1030Luini, W. 196, 257Lukehart, S.A. 321Lundén, R. 156Lundgren, K. 494Lundqvist, C. 894Lundqvist, H. 1, 3, 35,Luthe, D.S. 197Lutz, H.U. 584, 760, 761, 762, 764, 765Lycke, E. 156Lynch, M. 65, 158Lynch, T.J. 379Låås, T. 22Löfelhardt, W. 49Lörz, H. 165, 195

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MMa, D.D.F. 342MacDonald, R.M. 246, 247, 881Macfarlane, S.D. 672MacGlashan, Jr., D.W. 347Mackie, L.H. 701MacKinnon, S. 926, 1032Madden, E.A. 710Maddox, Y. 495Maddy, A.H. 77, 525Magee-Brown, R. 310Maggi, E. 421Maghni, K. 1146Magocsi, M. 1090Mahesh, V.B. 1159Maillard, J.L. 142Maisch, B. 198, 281Mak, N.K. 802Makarow, M. 565Makower, A.-M. 629Malcomnson, J.H. 1021Malinen, T. 923Mamelok, R.D. 143Manglitz, T. 1108Mann, L.C. 403Mannaerts, G.P. 426, 455, 474Manning, M.C. 244Mans, A. 260Maraini, G. 583Mardh, S. 569Margalioth, E.J. 1018Marí, M. 1160Marini, S. 934Marko, O. 380Maroux, S. 125Marques, E.K. 1039Marrs, R.P. 377Marsh, B.B. 103, 147Martial, E. 284Martin, H. 667Martin, N.J. 247Martin, S.J. 1071Martin, W.J. 603Marinez-Alonso, C. 144, 314Marinez-Masa, O. 427Marwaha, N. 601Marzella, L. 132, 145Marzilli, M. 1067Marzullo, A. 148Mas, J. 645, 646, 647Mask, P.L. 205Masmoudi, A. 657

Mason, R.R. 588Massaia, M. 342Masuda, K. 785Mateyko, G.M. 80Mathieu, C. 526Matsuda, M. 1081Matsui, H. 412Matsui, S. 805Matsumura, Y. 616, 631Matsuno, K. 428Matsushiro, A. 248Matthews, E.K. 605Matzku, S. 72Matzner, Y. 1115Maubon, I. 1020Mauger, J.-P. 1147Max, H. 1131May, A. 261May, S.A. 858, 930Mayorga, L.S. 1104Mazumoer, A. 429Mazur, S.P. 980Mbina, I. 852McCabe, Jr., M.J. 914McClure, R.D. 846McComb, D.J. 382McConkey, D. 954McCutchan, T.F. 510McDaniel, R.G. 185McGarry, R. 329McGinnes, K. 1031McGuire, L.A. 184McKegney, F.P. 317McKellar, S.B. 258McKenzie, I.F.C. 100, 160, 430McLaren, A. 367McLaren, G.D. 261McLaughlin, E.A. 1005McLean, D.J. 1111McMahon, D.G. 1138McMahon, G. 592McMahon-Pratt, D. 489McMillen, S. 904McNamara, B.C. 18, 146, 199Mead, G.P. 622Meistrich, M.L. 50, 207, 335, 381Mejia, E. 1087Meli, D. 297, 475Melinand, C. 1144Mendez, A.J. 1096Mendis, K.N. 854Merion, M. 406Merluzzi, V.J. 665

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Mersmann, G. 32, 365Mertens, N. 1128Meryman, H.T. 311Merz, W.E. 282Messick, J.B. 1075Metcalf, D. 70Meyer, D.I. 78Meyer, G. 606, 683Meyer, J. 803Meyer-Lehnert, H. 1145Michiels, C. 955Michihara, A. 1105Mickelsen, J.R. 103, 147Mier, J.W. 363Migas, I. 1145Milder, R.V. 400Miller, J.N. 481Miller, L.H. 263, 551Miller, R.A. 911Milligan, J.V. 19, 382Mills, W.R. 109Milsom, J. 13Mischeck, U. 803Miska, W. 1129Mita, K. 972Mitchell, G.F. 100, 119Miyake, M. 847Miyama-Inaba, M. 712Miyamoto, T. 801Miyasaka, N. 545Miyawaki, T. 283, 348Miyazaki, H. 1046Miyazawa, M. 693Mizobe, F. 284Mizuno, M. 1117Moav, N. 150Modenese, A. 933Moed, J.R. 566Moedder, E. 51Moest, D.R. 1050Moger, W.H. 997Mohanty, D. 601Mohri, H. 25, 1025Moingeon, P. 954Molitor, E. 982Moller, P. 905Molloy, M. 911Monconduit, M. 217Monner, D.A. 52, 419Montesoro, E. 499Moody, C.E. 277Moonen, G. 794Moore, A. 1000

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NNachman, R.L. 155Nadeau, D. 518, 555, 795Nagamori, S. 485Nagaoki, T. 283Nagasawa, S. 431Nagasawa, T. 285, 431Nagata, T. 795Nagauker-Shriker, O. 920Nagel, J. 984Nagel, M.-D. 984Nagpal, M.L. 992Nagy, A.K. 522, 786Nair, M.P.N. 385, 432, 562Nakata, M. 906Nakstad, B. 811Nameroff, M. 680Nandi, S. 222Naor, D. 449Narahara, H. 1051Navarro, L. 1098Neat, C.E. 53, 112, 200Neighbour, P.A. 323Neill, S. 1072Neitz, A.W.H. 848Nelson, D.A. 212Nelson, D.S. 130Nelson, K.D.G. 312Nemeth, A. 104, 640Nencioni, L. 433Nethanel, T. 150Neubert, T.A. 615Neuburger, M. 324, 651Neuhaus, H.E. 1126Neumann, C. 208Neumann, E. 186, 392Newhall, W.J. 434Newman, M.J. 1162Newman, W. 497, 430Newton, I. 1150Ng, M.C. 1042Niaudet, P. 48Nicolau, C. 644Nielsen, S. 970Nieschlag, E. 986Nieuwenhuis, P. 250Nii, Y. 1056Nijhof, W. 286, 386, 498Nillni, E.A. 151Nishimune, Y. 248Nishimura, M. 88Nishimura, T. 953

Nishioka, Y. 1051Nitta, T. 906Nnalue, N.A. 983Noble, N.A. 983Nocera, A. 499Noda, H. 1085Noelle, R.J. 513Nomoto, K. 639Nordli, O. 45Nordlind, K. 547, 602Nordlinder, H. 978Norlund, R. 883Norris, S.J. 593Noseworthy, J.H. 599Nossal, G.J.V. 160Nothacker, K.-D. 621, 842Nowakowski, B. 957Nunes, L. 846Nunes, S.P. 571Nunez, G. 215, 435Nusse, M. 943

OObexer, G. 346Obhrai, M.S. 1001Ogata, H. 1040Ogiso, Y. 248Ogren, W.L. 209Ogston, A.G. 46Ohene-Fianko, D. 567Ohlsson, L. 883Ohmann, H.B. 867Ohsumi, M. 1149Ohsumi, Y. 1149Ohyama, H. 221Ohzeki, S. 348Oka, T. 805Okada, M. 974Okano, T. 237Okpodu, C.M. 1125Okumura, M. 306Okuzawa, K. 1070Oldberg, Å, 29Oligati, L. 966Oliver, R.L. 201Olofsson, S. 156Olofsson, I. 98, 535, 1134Olsson, Y. 332, 517Olszewski, W.L. 818Onorato, J.J. 249

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Onvlee, P.C. 1078Opper, C. 790Opperdoes, F.R. 202Opstelten, D. 250Oram, J.F. 1096Orava, M. 682Orfao, A. 1109Orlando, C. 1015Orlic, D. 799Orlowski, J. 325Ortaldo, J.R. 243, 333, 351Ortiz, W. 58, 152Ortmann, R.A. 829Oshio, S. 25, 660, 679, 698, 839Osipovich, O.A. 917Oskam, R. 460Osmundsen, H. 53, 112, 200, 343, 444Ota, S. 809Ottonello, S. 583, 695Oursler, M.J. 585Outteridge, F.P.M. 251Overdulve, J.P. 269Overhauser, J. 874Overstreet, J.W. 1026Overwien, B. 366Owensby, D.A. 1130Oyaizu, N. 921

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Pauloin, A. 326Pawlowski, A. 629Pay, A. 766, 771Payrastre, B. 776Peake, I.R. 40Pearlmann, H. 452Pech, L.L. 1141Peck, A.B. 436Pedros-Alio, C. 642, 645, 647Peeler, K. 436Pelletier, M. 826Pellicer, A. 1160Pelling, J.C. 312Pembrey, R.G. 520Penington, D.G. 791Penney, J.B. 170Pennings, E.J.M. 287Penniston, J.T. 1090Percy, R.A. 66Perez, B.S. 1073, 1111Pericle, F. 950Perret, B. 54Perrot, J.Y. 48Perry, Jr., L.R. 231Perry, S. 126Persik, M.T. 1047Persson, A.-M. 1134Persson, A.V. 1153Pertoft, H. 1, 2, 3, 5, 6, 14, 17, 21,

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Philip, P.J.M. 369Phillips, G.L. 918Phillips, W.A. 500Pickering, B.T. 878Pickering, S.J. 861Piguet, H. 217Pike, B.L. 160Piovella, F. 40Pipeleers, D.G. 290Pipeleers-Marichal, M.A. 290

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Pisoni, R.L. 1102Pistoia, V. 892Plasman, N. 1054, 1055Plaza, A. 314Pleasure, D. 204, 244Ploch, S.A. 1099Plumley, D.A. 1089Pobor, G. 501Podevin, R.A. 469Podhajcer, O.L. 782Polak, P.E. 837Polentarutti, N. 924, 925Polgar, P.R. 824Pollack, S.B. 860Pollock, R.E. 907Popjak, J.G. 242Porwit-Ksiazek, A. 502Posner, B.I. 240, 318Potier, M. 362Potrykus, I. 195Poulter, L.W. 994Poupon, M.-F. 141Pousette, A. 662, 857Poxton, I.R. 1074Prasertsiriroj, V. 451Pratt, G. 946Preer, L.B. 1154Preer, Jr., J.R. 1154Pressman, D. 65, 158Preti, A. 871Pretlow, T.G. 1069Pretlow, T.P. 829, 1069Price, C.A. 28, 58, 60, 437Proctor, S.J. 476Prusiner, S.B. 143Przepiorka, D. 916Pulford, K. 154Pullman, W.E. 815Puppo, A. 708Pycock, J.F. 706

QQuan, P.C. 141Quatrano, R.S. 197Quillec, M. 533Qvigstad, E. 503, 504Qvinnan, G.V. 388

RRaaijmakers, J.A.M. 962Rabellino, E.M. 155Raff, H.V. 580

Raghuprasad, P.K. 438Raha, S. 790Rahmatullah, R. 1124Ralphs, J.R. 821Ramaraj, P. 996Ramirez, I. 207Ran, M. 150Rand, T.H. 344Ranki, A. 61, 67Rapoport, S.M. 784Rashleigh, S. 950Rask, L. 7Rasmussen, G.G. 352Rasmussen, J.M. 226, 352Rasmussen, R. 749Rasmussen, S.-E. 505Ratcliffe, M.J.H. 345, 506Ratcliffe, N.A. 622Ratner, D. 439Rauterberg, J. 71Ravnik, S. E. 860Rayment, C. 681Read, R.A. 956Reardon, E.M. 28, 58, 60, 437Rechsteiner, M. 946Record, M. 291, 633Reddehase, M.J. 507Reddy, J.K. 308Redecker, S. 316Ree, H.J. 898Reed, J. 393Reese, R.T. 447Regan, S. 1157Regelsberger, H.S. 600Rehm, H. 108Reibach, P.H. 205Reichert, A. 44, 192Reinero, A. 1122Reiners, J.J. 312Reinert, H. 117Reinhart, P.H. 327Remacle, J. 955Renard, D. 902Rendon, A. 657Rennie, C.M. 77Resnicoff, M. 720Rey, A. 254Rey, C. 687Reynolds, C.W. 252, 328, 333, 468Réz, G. 864Rhodes, J.M. 505Rhynhart, K. 900Riccardi, C. 458

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Ricetti, M. 40Richards, J. 222Richardson, J.C.W. 206Richburg, J. 979Richie, E.R. 207, 210Richter, E.R. 402Rickard, A. 1062Rickwook, D. 74, 75Riding, G.A. 253Rieber, P. 245Riemer, R.K. 1152Riethmüller, G. 99, 131Rikihisa, Y. 1075, 1076Rilfors, L. 1137Rimoldi, M.T. 691Risbridger, G.P. 804, 879, 889Rischert, J.C. 1138Rivadeneira, E. M. 440Rivas, D. 893Riveri, R.A. 554Rivoal, J. 1120Roberton, D.M. 527, 658, 666Roberts, A.I. 897Roberts, J. 1152Roberts, R. 573Robertson, D. 1125Robins, R.A. 266Robinson, P.J. 774, 887Roder, J.C. 329, 441Rodewald, H.-R. 954Rodriquez, S.D. 1080, 1083Roehlich, P. 640Roelofsen, B. 932Rogers, J. 605Rogge, H. 15Röhlich, P. 104Röllinghoff, M. 292Romagna, F. 798Romani, R. 383, 384Romeo, D. 148Ron, E.Z. 129Rönnemaa, T. 34Rook, A.H. 88Ropars, C. 939Rosati, F. 297Rose, L.M. 497Rosemiller, M.E. 473Rosenberg, I. 561Rosenberg, S.A. 429Rosenbloom, I.L. 255Rosenfeld, G.C. 178Ross, G.D. 155Ross, A.R. 950

Rothrock, J.K. 993Roussel, E. 1116Rousset, B. 827Roy, B. 355Rozmiarek, S.K. 1037Rubin, K. 23, 29Rubin, P. 441Rucheton, M. 254Rucknagel, D.L 935Rudberg, C. 107Rudich, A. 920Rudolph, S.A. 249Ruffmann, R. 225Ruggeri, Z.M. 626Ruiz-Navarro, A. 1142Rumley, A.G. 66Rumpold, H. 346Russel, P.J. 394, 1127Rüssmann, L. 293Russo, V. 940Rustin, P. 528, 852Ryan, N. 382Ryffel, B. 389, 1035Rylander, R. 797Röhlick, P. 104Röllinghoff, M. 292Rönnemaa, T. 34Rüssmann, L. 293

SSaad, A. 1019Sabater, J. 890Sabido, O. 1020Sackers, R.J. 168Sacks, D.L. 546Sacktor, B. 255Sadick, M.D. 580Sadovsky, Y. 1152Safai, B. 273Saida, T. 244Sakai, K. 834, 1056, 1057Saksela, E. 61, 67, 96, 162, 300Salgado, A.M. 1010Salisbury, J.G. 62Salvati, A.M. 350Salvo, G. 350Samoggia, P. 350San Miguel, J. 1109Sanbuissho, A. 1161Sanchez, P. 989Sandberg, G. 330Sandoval, C. 799Santini, P. 421

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Sarkar, N.H. 273Sasaki, T. 977Sato, K. 305Sato, M. 1079Sato, R. 256Satoh, K. 936Saul, A. 442Sautter, C. 294Sawae, Y. 1155Sbarbati, R. 723, 1067Scala, G. 550Scalera, V. 111Scarpa, A. 121, 267Schaal, J.P. 1150Schaffner, T. 428Scharp, D.W. 726Schat, K.A. 851Scheer, B.J. 1059Scherer, P. 632Scherlock, S. 13Schevnius, A. 443Scheynius, A. 443Schill, W.-B. 213Schiphorts, M.E. 298Schipper, L. 938Schirrmacher, V. 225Schleicher, R.L. 987Schleifer, S.J. 317Schleimer, R.P. 347Schmidt, G.W. 76Schmidt, H.J. 751Schmidt, W. 945Schmidt-Wolf, I.G.H. 1033Schmitt, C. 390Schneider, J. 138Scholz, W. 529Schoots, A.F.M. 168Schreier, M.H. 509, 542Schroff, G. 208Schroit, A.J. 942Schuh, D. 179Schulster, D. 315Schumacher, M. 31, 105Schwartz, L. 967Schwartz, R. 554Schwartz, S.A. 385, 432Schweizer, R.C. 962, 963Schwinghamer, P. 295Schwitzguébel, J.P. 296Schäfer, G. 31, 105Seale, T.W. 63Sedegah, M. 163Sedgwick, A.D. 617

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Singh, H. 728Sinn, W. 159Sixma, J.J. 298Sjöberg, O. 16, 83, 93, 175, 338Sjöstrand, M. 797Sjöström, L. 6Skalli, M. 989Skillrud, D.M. 603Skipsey, L.J. 948Skog, S. 557Skoog, V.T. 16Skottun, T. 238Skubitz, K.M. 511Slaga, T.J. 312Slone, J.H. 865Smedsrød, B. 650, 880Smit, N.P.M. 1132Smith, C.M. 127, 172, 173, 331Smith, D.J. 1099Smith, D.L. 490Smith, E.B. 172Smith, R.G. 215Smith, U. 6Smith, V.J. 445, 446Snider, J.M. 172Sniezek, M. 780Snow, E.C. 512, 513Snowden, R.T. 1064, 1065Snyder, D.S. 391Snyder, N.K. 912Snyderman, R. 268Sojar, H.T. 1077Soji, T. 306Soldateschi, D. 257Soldo, A.T. 689Somerville, C.R. 209Somerville, S.C. 209Sommerfeld, U. 425Somsel Rodman, J. 1107Sone, S. 903Sonthayanon, B. 1124Soome, A. 102Sordillo, L.M. 915Sorg, C. 39, 208, 230, 366Sorice, F. 297Souba, W.W. 1093, 1095Souhami, R.L. 154Spence, M.W. 596, 610, 806Spencer, Davies, P. 582Spillecke, F. 392Srivastava, C.H. 993Stahl, C.P. 1036, 1107Staite, N.D. 514

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Taniguchi, N. 283, 348Taniguchi, S. 1040Tanimoto, Y. 969, 971Tanner, M.J.A. 110Tanphaichitr, N. 863Taragelova, E. 361Tarcic, N. 449Targan, S.R. 372Tarkkanen, J. 300, 450Tarvis, A. 40Tatsumi, M. 789Tauro, G.P. 672Taylor, A.A. 301Taylor, W.M. 327Teale, J.M. 160Tencer, K.L. 411Tengi, J.D. 84Tengvar, C. 332, 517Terland, O. 89Testart, J. 1012Tettamanti, G. 792Tharavanij, S. 451Thierry, C. 254Thies, E.J. 1097Thies, W.-G. 556, 549Thom, E. 1126Thomas, E. 165Thomas, E.D. 916Thomas, N. 211Thomassen, M.S. 112, 200Thompson, J.E. 835Thompson, J.S. 77, 235Thompson, W.F. 841Thomson, A.B.R. 396Thorbecke, G.J. 228Thorn, N.A. 134Thorne, B. 870, 875Thorsby, E. 356, 357, 503, 504Thurston, R.J. 1073Tice, D.G. 212, 540Tietze, F. 787Timonen, T. 61, 67, 96, 162, 243, 252,

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Werkmeister, J. 329Wernicke, W. 165Werre, J.M. 938Wesemann, W. 157, 790West, K. 1014, 1016West, K.M. 859Weyers, J.D.B. 409Wha, B.I. 634White, C. 524White, T.C. 401Wide, L. 107Wielckens, K. 166Wierenga, H.P.K. 286, 386, 498Wierenga, P.K. 286Wigren, A. 241Wigzell, H. 16, 93, 175, 307, 338, 398, 436Wilkins, L.M. 167Wilkinson, A.E. 235Wilkinson, P.C. 508, 537Willadsen, P. 253Williams, N. 155Williams, R.J. 604Williams, S.K. 259Williamson, J. 1034Williamson, W.J. 184Willingham, M.C. 597Wilson, S.P. 220Winchester, R.J. 155Windle, B.E. 136Wingren, A. 560Winkler, H.H. 825Winqvist, I. 535Winton, E.F. 1037Wiser, M.F. 1082Witz, I.P. 150Woerly, G. 1035Woldringh, C.L. 260Wolff, D.A. 82Wolinsky, J.S. 170Wolos, J.A. 224Wolowyk, M.W. 407, 462Wong, C.C. 802Wong, G.L. 1042Wong, P.S.K. 1162Wonnacott, S. 870, 875Woody, J.N. 340Worman, C. 173Worwood, M. 534Wreford, N.G.M. 649Wu, C.H. 985Wu, G.Y. 985Wunderlich, F. 685, 692Wyszynski, R.E. 699

Wärmegård, B. 24, 25, 33, 57

XXu, Q.P. 833

YYabe, M. 789Yablonka-Reuveni, Z. 680Yachie, A. 283, 348Yaffe, H. 1018Yagi, H. 716Yagi, Y. 730Yagita, H. 906Yakymyshyn, L.M: 396Yamada, T. 221Yamada, Y.K. 789Yamaguchi, H. 1123Yamaguchi, J. 648Yamaguchi, T. 305, 638Yamamoto, H. 465Yamamoto, K. 822Yamamoto, M. 913Yamamoto, T. 306Yanagida, M. 1046Yanagita, Y. 953Yancovitz, S.R. 898Yang, C.Y. 824Yang, J. 222Yashiro, K. 1117Yasumizu, R. 1041Yau-Heiu, H. 1086Yeltman, D.R. 120, 191Yeung, W.S. 878Yodoi, J. 487Yokoi, T. 283, 348Yoshida, H. 936Yoshimura, N. 805Yoshizaki, N. 168Young, J.D. 461, 462, 609Yu, C. 378Yu, C.-T. 1049Yung, Y.P. 463

ZZagury, D. 254Zajac, I, 368Zaleski, J. 979Zamora, P. 1087Zamorano, J. 893Zampini, P. 164Zanen, J.G. 287Zanjani, E.D. 671

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Zaytoun, A.M. 375Zeligs, B. 495Zhang, H. 928Zhang, M. 987Zhang, Y. 1076Zheng, M. 987Ziebe, S. 1004Ziegler, A. 108Zon, Y. 613Zucker-Franklin, D. 1036Zupo, S. 896Zwain, I.H. 999Zöller, M. 72, 307, 397, 398, 399

ÅÅkerström, G. 1, 2, 3, 27, 35, 107Åman, P. 502, 538

ÖÖbrink, G. 29, 33Örn, A. 83Östberg, L. 83

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Ordering Information

Percoll is a gradient medium specially designed to separate cells, subcellular particles and largerviruses under gentle conditions. It is sterile, non-toxic to cells and can be used to prepare gradientscovering a range of densities up to 1.3 g/ml. Density gradients can either be pre-formed usingstandard mixing techniques or formed spontaneously by centrifuging Percoll at moderate centrifugalforces in an angle-head rotor. Percoll is easily adjusted to be iso-osmotic with the cells or particles tobe separated, and its low viscosity means that cell separations on pre-formed gradients can becompleted in only a few minutes using low centrifugal forces (200-1000 x gav). Even relativelyfragile cells retain viability after separation on gradients of Percoll due to the gentle conditions used.

Percoll can be used to separate intact cells, subcelular particles and viruses down to the size ofappoximately 70S.

Panel A. Separation of humanblood cells in a gradient ofPercoll. Bottom layer containsred blood cells, the middle bandis polymorphonuclear cells(e.g. granulocytes) and the topband is mononuclear cells.

Panel B: Percoll, our exceptionaldensity gradient medium, isnow available in easy to open,resealable bottles.

Products for Cell Separation and Culture

PrPrPrPrProductoductoductoductoduct QuantityQuantityQuantityQuantityQuantity Code No.Code No.Code No.Code No.Code No.

Percoll™ 1 l, sterile 17-0891-01

250 ml, sterile 17-0891-02

Density Marker Beads 10 vials 17-0459-01

Ficoll-Paque™ PLUS 6 x 100 ml 17-1440-02

6 x 500 ml 17-1440-03

Ficoll™ 400 100 g 17-0400-01

500 g 17-0400-02

Cytodex™ 1 25 g 17-0448-01

Cytodex™ 3 10 g 17-0485-01

Dextran Sulphate 100 g 17-0340-01

500 g 17-0340-02

Density Marker Beads are small coloredbeads with predetermined densities, usedfor calibrating gradients of Percoll. Theyare supplied as a kit containing 10 differenttypes of beads covering the normalworking range of densities obtainablewith Percoll. The beads are extremelysimple to use and permit rapid andprecise determination of the buoyantdensities of cells separated in densitygradients of Percoll.

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Products for Purification of DNA

PrPrPrPrProductoductoductoductoduct QuantityQuantityQuantityQuantityQuantity Code No.Code No.Code No.Code No.Code No.

RapidPrep™ Micro Genomic 1 kit† 27-5225-01

(DNA Isolation Kit for Cells and Tissue)

RapidPrep™ Macro Genomic 1 kit† 27-5230-01

(DNA Isolation Kit for Cells and Tissue)

FlexiPrep™ Kit 1 kit† 27-9281-01

Kits for cDNA Synthesis

PrPrPrPrProductoductoductoductoduct QuantityQuantityQuantityQuantityQuantity Code No.Code No.Code No.Code No.Code No.

TimeSaver™ cDNA Synthesis Kit (5 reactions) 1 kit† 27-9262-01

cDNA Synthesis Kit (5 reactions) 1 kit† 27-9260-01

First-Strand cDNA Synthesis Kit (55 reactions) 1 kit† 27-9261-01

Ready-To-Go™ T-Primed First-Strand Kit 1 kit† 27-9263-01

(50 reactions)† Product must be shipped cold. There is an extra charge for insulated container and refrigerant.

Products for Purification of RNA

PrPrPrPrProductionoductionoductionoductionoduction QuantityQuantityQuantityQuantityQuantity Code No.Code No.Code No.Code No.Code No.

QuickPrep™ Micro mRNA Purification Kit 1 kit† 27-9255-01

(24 purifications)

QuickPrep™ mRNA Purification Kit 1 kit† 27-9254-01

(4 purifications)

QuickPrep™ Total RNA Extraction Kit 1 kit† 27-9271-01

RNA Extraction Kit 1 kit† 27-9270-01

(extracts RNA fromup to 4 g of tissue)

mRNA Purification Kit

(2 purifications) 1 kit† 27-9258-01

(4 purifications) 1 kit† 27-9258-02

CsTFA (Solution) 100 ml 17-0847-02

Oligo(dT)-Cellulose 500 mg 27-5543-01

Type 7 1 g 27-5543-02

5 g 27-5543-03

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Percoll, Sephadex, Sephacyl, Ficoll, Ficoll-Paque, FlexiPrep, Cytodex, QuickPrep, TimeSaver, RapidPrep and Ready-To-Goare trademarks of Amersham Biosciences Limited.

Amersham is a trademark of Amersham plc.

The data presented herein have been carefully complied from our records, which we believe to be accurate and reliable.We make, however, no warranties or representations with respect hereto, nor is freedom from any patent to be inferred.Before any part of this manual is reproduced, please request permission from Amersham Biosciences.

The products described in this literature are intended for in vitro use only. Nothing in this literature should be construed aseither a recommendation or an authorization to use these products for in vivo applications.All goods and services are sold subject to the terms and conditions of sale of the company withinthe Amersham Biosciences group that supplies them.

A copy of these terms and conditions is available on request.© Amersham Biosciences AB 2001 – All rights reserved.

Amersham Biosciences AB Björkgatan 30, SE-751 84 Uppsala, Sweden

Amersham Biosciences Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA, EnglandAmersham Biosciences Inc 800 Centennial Avenue, PO Box 1327, Piscataway, NJ 08855 USA

Amersham Biosciences Europe GmbH Munzinger Strasse 9, D-79111 Freiburg, Germany

Amersham Biosciences KK, Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073 Japan

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