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PCR Methods and Applications A New Interdisciplinary Journal of Research, Methods, Reviews, and Comment [ Scientists have seized vigorously on the [ power and flexibility of the polymerase ~.~..~ ] chain reaction (PCR), and this enthusiasm ~ ~ , ° [ ;~4~:~:~. [ is generating a host of PCR-based and ~2.~. ~ ~ . . . . [ "~ . [ other amphficatlon techmques as well as [ G [ an extraordinary range of applications. Re- ~ [ ports of these advances are currently scat- [ tered throughout the literature. The new international journal PCR Methods and Appli- cations is the first peer-reviewed journal de- voted exclusively to amplification methods and their use, providing a central source of reliable, independent, up-to-date information that investigators in every discipline can use. PCR Methods and Applications publishes refereed research papers detailing improvements in PCR methodology and new amplification techniques, as well as papers describing the application of these methods to a wide variety of research problems. These papers are supplemented by commissioned review articles, commentaries, technical tips, and reader correspondence. Early issues of the journal will be widely distributed among PCR users. Review articles in these issues cover topics such as: • methods for sequencing PCR products • PCR and environmental pathogens • PCR-based HLA class II typing • the ligase chain reaction • sequence tagged sites and genomic mapping • PCR techniques in phylogenetic studies • ALU PCR • PCR and the molecular diagnosis of cancer • PCR analysis of ancient DNA • single-stranded conformation polymorphisms • PCR detection of mutations • HPV detection and analysis with PCR SUBSCRIPTION INFORMATION Volume 1, 1991/1992 - containing 4 quarterly issues (beginning August, 1991) Individual Price (U.S.)$40 Individual Price (R.O.W.airlift delivery) $50 Institutional Price (U.S.)$120 Institutional Price (R.O.W. airlift delivery) $150 TO ORDER Call: 1-800-843-4388 (continental U.S. & Canada) 516-349-1930 (all other locations) FAX: 516-349-1946 Write: Cold Spring Harbor Laboratory Press 10 Skyline Drive Plainview, NY 11803-9729 Orders for India: Panima Educational Book Agency, C-8, Safdarjang Development Area, Shopping Centre, Hauz Khas, New Delhi-110016 India. Orders for Japan: Maruzen Company LTD., 3-10, Nihonbashi 2- Chome, Chou-ku, Tokyo, 103 Japan. Orders for Hong Kong, Korea, Malaysia, Singapore, Taiwan, and Thailand: Info Access & Distribution Pte. Ltd., 14 Conway Grove, Singapore 1955. Reader Service No. 813 Cold Spring Harbor Laboratory Press G&D2

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Page 1: PCR Methods and Applications - Genes & Developmentgenesdev.cshlp.org/content/6/8/local/back-matter.pdfPCR Methods and Applications publishes refereed research papers detailing improvements

PCR Methods and Applications A New Interdisciplinary Journal of Research, Methods, Reviews, and Comment

[ Scientists have seized vigorously on the [ power and flexibility of the polymerase

~.~..~ ] chain reaction (PCR), and this enthusiasm ~ ~ , °

[ ;~4~:~:~. [ is generating a host of PCR-based and ~2.~. ~ ~ . . . . [ " ~ . [ other amphficatlon techmques as well as [ G [ an extraordinary range of applications. Re-

~ [ ports of these advances are currently scat- [ tered throughout the literature. The new

international journal PCR Methods and Appli- cations is the first peer-reviewed journal de-

voted exclusively to amplification methods and their use, providing a central source of reliable, independent, up-to-date information that investigators in every discipline can use.

PCR Methods and Applications publishes refereed research papers detailing improvements in PCR methodology and new amplification techniques, as well as papers describing the application of these methods to a wide variety of research problems. These papers are supplemented by commissioned review articles, commentaries, technical tips, and reader correspondence.

Early issues of the journal will be widely distributed among PCR users. Review articles in these issues cover topics such as:

• methods for sequencing PCR products

• PCR and environmental pathogens

• PCR-based HLA class II typing

• the ligase chain reaction

• sequence tagged sites and genomic mapping

• PCR techniques in phylogenetic studies

• ALU PCR

• PCR and the molecular diagnosis of cancer

• PCR analysis of ancient DNA

• single-stranded conformation polymorphisms

• PCR detection of mutations

• HPV detection and analysis with PCR

SUBSCRIPTION INFORMATION

Volume 1, 1991/1992 - containing 4 quarterly issues (beginning August, 1991)

Individual Price (U.S.) $40 Individual Price (R.O.W. airlift delivery) $50 Institutional Price (U.S.) $120 Institutional Price (R.O.W. airlift delivery) $150

TO ORDER

Call: 1-800-843-4388 (continental U.S. & Canada) 516-349-1930 (all other locations)

FAX: 516-349-1946 Write: Cold Spring Harbor

Laboratory Press 10 Skyline Drive Plainview, NY 11803-9729

Orders for India: Panima Educational Book Agency, C-8, Safdarjang Development Area, Shopping Centre, Hauz Khas, New Delhi-110016 India. Orders for Japan: Maruzen Company LTD., 3-10, Nihonbashi 2- Chome, Chou-ku, Tokyo, 103 Japan. Orders for Hong Kong, Korea, Malaysia, Singapore, Taiwan, and Thailand: Info Access & Distribution Pte. Ltd., 14 Conway Grove, Singapore 1955.

Reader Service No. 813

Cold Spring Harbor Laboratory Press

G&D2

Page 2: PCR Methods and Applications - Genes & Developmentgenesdev.cshlp.org/content/6/8/local/back-matter.pdfPCR Methods and Applications publishes refereed research papers detailing improvements

YES, QUALITY IS YOUR FIRST CHOICE

FOR RNase HI!

Purity: Single Band on PAGE

Easy to Use: 1 - 2 units / !~1

Convenient: 3 Package Size available 50, 100 and 400 units

1 2 3 4 5 6 phosphorylase B

bovine serum albumin

ovalbumin

carbonic anhydrase

soybean trypsin inhibitor

lysozyme

Lane 1. The protein standards for molecular weight markers 2. The total soluble proteins from E. coli clone 3. The proteins purified by the first ion exchange chromatography 4. The more purified proteins by the second ion exchange chromatography 5. The purified RNase H by the final purification of gel filtration

No other protein appeared on PAGE 6. Same as Lane I

R ibonuc lease H from E. coli is an endonuclease that specifically degrades the RNA strand of DNA-RNA hybrids. RNase H is a key enzyme in several procedures including:

- Amplif ication by self-sustained sequence replication (3SR) - cDNA synthesis and cloning where it is used to remove mRNA during second strand cDNA synthesis - Removal of poly (A) tails of mRNAs after hybridization with poly dT

"RESEARCH USE ONLY. NOT FOR DRUG OR OTHER USES"

S E I K A G A K U AMERICA, INC. 30 West Gude Drive, Suite 260 Rockville, Maryland 20850-1161, U.S.A. Tel (301) 424-0546 • 1-800-237-4512 Fax (301) 424-6961

S E I K A G A K U CORPORATION Tokyo Yakugyo Bldg. 1-5, Nihonbashi-Honcho 2-Chome Chuo-Ku, Tokyo, 103 Japan Tel (03) 3 245-1951 • FAX (03) 3 242-5335

Reader Service No. 814