onsite hev rapid test. acute viral hepatitis transmitted via fecal-oral route jaundice in 50% cases...
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OnSite HEV Rapid Test
Acute viral hepatitis Transmitted via fecal-oral route Jaundice in 50% cases Clinical symptoms Supportive therapeutics
Hepatitis A and Hepatitis E
SimilaritiesBut they are different diseases!
Different Viral Family
ssRNA picornavirusSingle serotype worldwideVaccine is available
ssRNA hepevirus1 serotype, 4 genotypesVaccine is being developed
Hepatitis A Virus Hepatitis E Virus
Different Transmission Routes
Close personal contact household contact homosexual in men child day care centers
Contaminated food, water infected food handlers raw shellfish
Rarely by blood exposure intravenous drug use blood transfusion
Contaminated food and water
Rarely from person to person
Hepatitis A Hepatitis E
Possible zoonotic transmission
Different Incubation Times
15-60 days, average 50 days 15-45 days, average 30 days
Hepatitis A Hepatitis E
Different Population
Symptomatic infection is common
in young adults between 15-40 yrs
The most common acute viral hepatitis.
Affects all age groups, Particularly common in children & young adults
Found primarily in Asian, Africa and South America
Hepatitis A Hepatitis E
Asymptomatic or atypical infection seen mostly in children
Genotype 1: 15-35 yrs Genotype 3-4: Japanese >60 yrs Genotype 1-2: human Genotype 3-4: human & animal
Different Disease Course
1-2% fatality rate, but could go up to 75% with chronic liver disease 20% in the 3rd trimester pregnant women up to 33% of premature infants
Self limiting, no reports of chronic status
Complications: Fulminant hepatitis Cholestatic hepatitis Relapsing hepatitis
0.4% fatality rate
HAV and HEV co-infection in young children may lead to LIVER FAILURE
Complications: Fulminant hepatitis Hepatic cirrhosis in these with immuno- compromised conditions
Self limiting, chronic status in these with immuno-compromised conditions
Mild illness
Mild illness in most cases
Hepatitis A Hepatitis E
Different IgM Generation Time
Appears in the 1st week of infection Peaks at 4 weeks Detectable up to 2-3 months
Appears in the 2nd week of infection Peaks at 7 weeks Detectable up to 4 months
Hepatitis A Hepatitis E
IgM detection is the most common specific diagnostics
Current Clinical Status of HAV and HEV
HEV is often under diagnosed or misdiagnosed: HAV is the most common acute viral hepatitis Lack of awareness of the differences between HEV and HAV Lack of awareness = Diagnostic kits are unavailable, in particular,
HEV test kits are not well-known on the market
Differential diagnosis of HEV and HAV will save lives
HEV is more severe than HAV in high risk groups & requires prompt action to prevent disease progression
HAV and HEV co-infection in young children may lead to liver failure!
CTK Biotech Offers
Easy to use screening kits OnSite HAV IgM Rapid Test kit OnSite HEV IgM Rapid Test kit
Complimenting confirmation kits HAV IgM ELISA kit RecimbilisA HEV IgM ELISA kit
To aid in differential diagnosis of HEV and HAV
OnSite HAV IgM Rapid Test Procedure
1 drop ofspecimen
1 drop ofsample diluent
Resultin 15 minutes!
CE
As easy as “1-2-3” Minimal training required No equipment needed Fast and reliable result in 15 minutes Can be used in any setting
OnSite HAV IgM Rapid Test CE
Test Result Interpretation
NegativeNo HAV Infection
PositiveCurrent infection or late stage of infection
InvalidRepeat test
OnSite HAV IgM Rapid Test CE
CTK HAV IgM Rapid Test
Reference Positive Negative Total
Positive 21 1 22
Negative 0 178 178
Total 21 179 200
Performance
Only 15 minutes to get results…. Relative sensitivity: 95.5% Relative specificity: 100%
CTK HAV IgM ELISA Kit CE
Catalog: E0100ADAPTABLE TO AUTOMATED SYSTEMS
CTK HAV IgM ELISA kit CE
CTK HAV IgM ELISA
Reference Positive Negative Total
Positive 39 0 39
Negative 0 361 361
Total 39 361 400
Performance
Only 70 minutes to get results…. Relative sensitivity: 100% Relative specificity: 100%
OnSite HEV IgM Rapid Test CE
Catalog: R0095C
OnSite HEV IgM Rapid Test Procedure
1 drop ofspecimen
1 drop ofsample diluent
Resultin 15 minutes!
CE
As easy as “1-2-3” Minimal training required No equipment needed Fast and reliable results in 15 minutes Can be used in any setting
OnSite HEV IgM Rapid Test Result
Test Result Interpretation
NegativeNo HEV Infection
PositiveCurrent infection or late stage of infection
InvalidRepeat test
OnSite HEV IgM Rapid Test CE
CTK HEV IgM Rapid Test
Reference Positive Negative Total
Positive 314 6 320
Negative 6 734 740
Total 320 740 1060
Performance
Only 15 minutes to get results…. Relative sensitivity: 98.1% Relative specificity: 99.2%
RecombiLISA HEV IgM ELISA Kit CE
Catalog: E0105ADAPTABLE TO AUTOMATED SYSTEMS
RecombiLISA HEV IgM ELISA kit CE
CTK HEV IgM ELISA
Reference Positive Negative Total
Positive 25 0 25
Negative 0 375 375
Total 25 375 400
Performance
Only 75 minutes to get results…. Relative sensitivity: 100% Relative specificity: 100%
Final Messages to Doctors
HAV and HEV seem similar BUT are clinically different
HEV could be life threatening and requires prompt action to prevent disease progression
HEV is often under diagnosed or misdiagnosed
Differential diagnosis of HEV and HAV is clinically significant
Final Messages to Doctors
OnSite HAV IgM Rapid Test Cat: R0090C
For easy, on site screening & differentiation of HAV & HEV
Order CTK Biotech’sOnSite HEV IgM Rapid Test Cat: R0095C
Final Messages to Doctors
HAV IgM ELISA kit Cat: E0100C
For further confirmation & differentiation of HAV & HEV
Order CTK Biotech’s
HEV IgM ELISA kit Cat: E0105C
Together, we grow Athenese Dx & CTK
Early Diagnosis for a better life
Together, we grow Athenese Dx & CTK
Athenese DX contact information:
Module # 407 & 408, 4th Floor,Ticel Bio Park II, No. 5, CSIR Road,
Taramani, Chennai - 600113,TamilNadu, India.