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Nghin cu thnh phn v tnh cht ca polysaccharide tch chit t nm Linh chi -Ganoderma lucidumnui trng ti tnh Tha Thin Hu( phn 1)

I. M uLinh chi l mt loi tho dc thuc chi Ganoderma, c s dng t xa xa trong y

hc c truyn Trung Quc v cc nc ng . Theo ho dc hin i thGanoderma

c kh nng ngn chn v cha mt s bnh nh ung th, cao huyt p, khng oxy ho,AIDS v cc bnh v tim mch,Cng vi triterpenoids, polysaccharide (PS) l mttrong cc thnh phn quan trng to nn hot tnh sinh hc k diu ca nm Linh chi. Ccnghin cu gn y cho thy: hot tnh sinh hc ca polysaccharide ph thuc rt nhiuvo thnh phn v cu trc. Trong phn ny, chng ti nghin cu thnh phn ca

polysaccharide tch chit t nm Linh chi Ganoderma lucidumtrng ti hp tc xPh Lng, huyn Ph Vang, tnh Tha Thin Hu.2. Thc nghim

2.1. i tng nghin cu

Mu nm Linh chi G. lucidum c trng ti hp tc x Ph Lng I, huyn PhVang, tnh Tha Thin Hu.2.2. Phng php tch chit PS

Sau khi thu hi, nm c sy kh 60 0C loi cc enzyme v xc nh mbng phng php khi lng. Tin hnh tch chit polysaccharidebng nc ct 1000C. Dch chit c lc qua giy lc, sau c quay gim th tch.Kt ta hon ton

polysaccharide bng EtOH nng 900

. Kt ta c tch ra bng cch ly tm (4000vng/pht trong 20 pht), lm kh 40 0C, thu c bt PS.

2.3. Phng php tinh ch PS

Thm tch bt PS nhiu ln bng nc ct loi b cc tp cht l cc phn t nhtan trong nc.S dng hn hp CHCl3 : n-C4H9OH = 1 : 4 (v/v) loi protein. DngEtOH 960 kt ta li PS.Tip tc ra bng acetone, sau l EtOH tuyt i nhiu ln loi b tp cht.

2.4. Cc phng php phn tch

2.4.1. nh tnh v nh lng polysaccharide

Qu trnh chit polysaccharide kt thc khi dch chit trong sut khng mu vi thucth phenol acid sulphuric.

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Hm lng polysaccharide c xc nh theo phng php Dubois, s dng D-glucose lm cht chun. Bt PS c ho tan bng nc ct, sau to mu vi thuc

th phenol acid sulphuric v tin hnh trc quang bc sng = 490 nm.2.4.2. Thnh phn ca polysaccharide

Hm lng protein c trong bt PS c xc nh bng phng php Lowry, s dngAlbumin protein ca huyt thanh lm cht chun. Acid

phosphomolypdatephosphotungstic trong thuc th Folin-Ciocalteu phenol s b khthnh heteropolymolybdeum nh qu trnh oxy ha s dng xc tc ng ca ccaminoacid cha vng thm trong protein. Sn phm to thnh c mu xanh hp th cci ti bc sng 750 nm.

Phng php khi lng xc nh m, tro c trong bt PS .3. Kt qu v tho lun

Hm

lng bt PS tch chit c t mu nguyn liu v tnh cht ca bt PS

Bt PS thu c c mu vng nht, mi thm du nh, v ngt; tan trong nc chodung dch mu nu, tan trong dung mi dimethylsulfoxide khi khuy v gia nhit chomu vng m; khng tan trong EtOH 960cho kt ta mu nu m. PS cho mu vng dacam khi tc dng vi phenol-acid sulphuric. Hm lng bt PS thu c so vi hm khca mu G. lucidum l 4,42 0,13% (P= 0,95; n= 6).

Vi phng trnh ng chun: Y = 0,0082 X0,0004 viR= 0,9999, trong khong

nng D-glucose t 0 n 125 g/mL, chng ti xc nh c hm lng PS trongmu G. lucidum trng ti x Ph Lng, huyn Ph Vang, tnh Tha Thin Hu l 3,93

0,03% (P= 0,95; n= 6). Kt qu x l s liu cho thy RSD < RSDH, nh vy cc thnghim c lp li tt. Vy, bt PS th thu c c hm lng PS tinh khit l 88,91 0,13% (P= 0,95; n= 6), hm lng protein 9,69 0,09% (P=0,95; n= 6), Hm lng trotng ng vi thnh phn khong: 6,81 0,21% (P=0,95; n= 3).