new software features of the 7010 particle sizer.ppt · pdf file · 2016-08-30new...

Danielle ChamberlinMay 5 2009

New software features of the 7010 Particle Size May 5, 2009of the 7010 Particle Size

Analyzer 2.0.0

Introduction to the new 7010 software

•Instrument operation

•New look and feel – easy to operate document formatNew look and feel easy to operate document format

• Data display options

•Advanced features

• Restricting wavelength/particle size range - notch• Particle fit algorithm choices• Time SeriesTime Series• Stitch

•The materials library and editing RI

•Reporting

•Licensing

Installation

1. Find Agilent 82357B USB-GPIB cable

2 Plug in GPIB end to instrument2. Plug in GPIB end to instrument

3. Plug USB end into computer

Power on instrument

Instrument operation

The 7010 software now directly controls the 7010 instrument. There is no need to run Chemstation in the background. Three i l b tt ll th t’ i d t k tsimple buttons are all that’s required to make a measurement.

Turning the lamps on

Lamp icon

When the software is first started, the lightbulb icon turns on. The “warming” state will display. The lamps must warm for 10 g p y pminutes before measurement is enabled. This ensures the lamps are stable and measurements are valid.

Blank and sample measurementblankblank

sample

After the 10-minute warmup period, the lamp icon changes to a f ll lit t t Th “bl k” i ( tt ith li id b tfully lit state. The “blank” icon (cuvette with liquid but no particles) will now become enabled. After a blank is measured, the “sample” icon (cuvette with liquid and particles in it) will become enabled.

Making a blank measurement

1. Fill cuvette with water or suspending fluid

2. Insert into sample holder

3. Click “blank” icon in 7010 software

Blank measurement

Wh bl k i d i d ill di l i thWhen a blank is measured, a window will pop up displaying the difference between two successive blank measurements. If the lamps are stable, this “noise” measurement should be low p(<0.001 AU). This popup may be clicked away after review.

Making particle size measurements

When the “measure” icon is clicked, a spectrum is measured , pfrom the 7010 hardware. The particle type, fluid, and pathlength must be selected for the software to calculate particle size.

Signal saturation

The new 7010 software warns the user when they have taken a bad measurement that saturates the detector. The user is advised that they may wish to dilute or shorten the pathlength.advised that they may wish to dilute or shorten the pathlength.

Graphical User Interface

The 7010 PSA software uses a document format to betterThe 7010 PSA software uses a document format to better match the user’s workflow. Rather than “one sample per file” a logical set of measurements can be combined together in one file for easy reviewfile for easy review.

Editing measurement names

When a measurement is made, a time and date stamp will appear as the ID p ppfor that measurement in the spectrum list view. Clicking once will select that spectrum to be viewed in the spectrum view and particle size view. Clicking a second time on the time/date stamp will allow the user to edit the spectrum ID to a more descriptive name.

Measurement comments

Additional annotations about a particular measurement may beAdditional annotations about a particular measurement may be made by right clicking on the spectrum name in the spectrum list view and selecting “Properties.”

Comparing spectra and PSD measurements

Simply shift-click or control-click on multiple spectrum names to compare themcompare them.

Drag and drop

S t b d d d d d b t d t d d ithiSpectra can be dragged and dropped between documents, or dragged within a document to re-order. Spectra can also be copied and pasted within or between documents, and deleted, by menu commands or the familiar Ctrl-C, Ctrl V Ctrl X (or Del) key commandsCtrl-V, Ctrl-X (or Del) key commands.

Drag and Drop

Data display options

Data can be displayed in concentration vs size, cumulative concentration vs size, or both overlaid. It can be displayed as volume density or number density.

Mean particle size view

The particle size view can also display the mean particle size of all spectra in the document (or all selected spectra) as a bar chart This is especially useful in a time series measurementchart. This is especially useful in a time series measurement.

Restricting wavelength range

Due to absorbing molecules in the fluid, it is sometimes necessary to remove certain wavelengths from the particle size calculation. This can be done through the menu bar under “Data” or by right-clicking anywhere in the g y g g yspectrum view window.

Restricting wavelength range (2)

When the wavelength range is restricted sliders appear at theWhen the wavelength range is restricted, sliders appear at the top of the spectrum view which can be moved to further adjust the wavelength range. De-selected wavelengths appear grey on the screen.

Notch

If you would like to remove wavelengths in the middle of the spectrum, use the “notch” feature.

Choice of algorithms to fit data – UV Autocorrect

Often there are UV-absorbing molecules in the fluid phase which are not correctly modeled in the fit. By default, the 7010 algorithm ignores these wavelengths if doing so improves the fit. If there are small particles in your sample you may need to turn this feature offsample, you may need to turn this feature off.

Calculation options window

Deselect the checkbox marked “Automatically Adjust for Poor UV Fit” and click “OK.”

UV Autocorrect

Now the population of 100 nm particles in the sample shows up. p p p p pYou may wish to make a copy of the measurement before altering calculation options so you can easily see the effect.

Calculation options

The 7010 particle size fitting algorithms use a variety of proprietary approaches to fit the measured data. In default mode, the computer automatically reports the calculation with the best fit to the measured spectrum. If the user wishes to force the particle size distribution to be monodisperse, fit a Gaussian profile, or expects the distribution to be broad they may force the algorithm to use this fit in the “calculationdistribution to be broad, they may force the algorithm to use this fit in the calculation options” window.

Time Series

The fast data acquisition time of the 7010 Particle Size Analyzer allows measurements of fast-changing samples not possible with other particle size measurement techniques.

•To acquire a time series, first, measure a blank.

•Then insert your sample. Select Instrument -> “Time Series.”

•Type the total run time desired, the number of samples, and

linear or log spacing between measurements in time.

StitchFor most measurements the high dynamic range of the 7010 is sufficient.

For measurements which contain extremely high attenuations along with extremely low attenuations where it is important to accurately measure both, the “Stitch” feature allows you to take measurements with two different path length cuvettes andwith two different path length cuvettes and combine them.

Simply select two measurements of the same sample with different pathlengths,same sample with different pathlengths, and go to Data->Advanced->Stitch.

Click the cursor (vertical red line) at the wavelength where you wish to stitch thewavelength where you wish to stitch the two spectra together. The longer pathlength sample will be used to the right of the red line, the shorter pathlength to the leftleft.

The Materials Library

The 7010 Particle Size Analyzer has a large list of materials to choose from in its materials library. To see the real and imaginary refractive indices used in the particle sizeimaginary refractive indices used in the particle size calculations, go to Data -> Advanced -> Materials Library.

Adding and editing materials

The materials library allows you to edit existing refractive index files and add your own. y

For example, you may copy an existing RI file to one with a new name, then edit the new RI file.

Editing RI

You may edit the RI by simply clicking in the data spreadsheetYou may edit the RI by simply clicking in the data spreadsheet and typing in new values. Once the values have been changed, the floppy disk “Save” icon becomes orange and

ill hi hli h d il i l i dwill stay highlighted until your new material is saved.

Making a new material

You can enter an entirely new refractive index into your ymaterials library. To do this, click on the gold “plus” icon, and enter the new material’sand enter the new material s name.

The refractive index numbersThe refractive index numbers can be typed in directly, or simply be cut and pasted from three columns in an Excelthree columns in an Excel spreadsheet containing wavelength, n, and k.

Measuring k

If the particle phase of your sample can be made into a fluid, for example if your sample is an emulsion or particles in your dispersion can be dissolved, k may be measured directly by the absorbance measurement

th 7010on the 7010.

Deselect any particle or fluid selections, take a blank (either of water if your sample is a fluid, or of the solvent if your sample is dissolved). The resulting spectral attenuation measurement can be exported under “File-> Export Selected Spectrum Data.”

The imaginary RI for a pure fluid can be calculated from the absorptionThe imaginary RI for a pure fluid can be calculated from the absorption measurement as where A is absorption and l is the pathlength.

For a solution the k of the solute is where C is the volume

lAkπλ

4=

Ak λFor a solution the k of the solute is where Cv is the volume concentration. vlC

kπ4

=

Transparent materials

The refractive index of transparent materials is well-modeled by the Sellmeier equation. If no k data is entered, the user has a h i t h th ft d t fchoice to have the software use raw n data or perform a

Sellmeier fit to the data. If a Sellmeier fit is performed, the wavelength range will be extended to 190 nm and 1100 nm g geven if the entered data does not extend over this range.

Sharing custom RI files

If you make a custom RI file and wish yto share your particle analysis result with another customer, you will need to also share the refractive index filealso share the refractive index file. The import and export icons in the Materials Library allow you to share

f fthese .rfi files.

Reporting

The software can generate a report of the measured data. To e so a e ca ge e a e a epo o e easu ed da a oaccess reporting features, go to File-> Generate Report or simply press Ctrl-P.

Licensing

Your 7010 software may be copied onto as many computers as you wish without y p ycontrol of an instrument, simply for data analysis.

For instrument control, a valid license key must be present.

T bt i th li k ill dTo obtain the license key, you will need your license entitlement certificate, shipped with your 7010 instrument. It pp yshould look something like the picture at right.

Adding a licenseTo obtain the license go to the Agilent Licensing Website either by clickingTo obtain the license, go to the Agilent Licensing Website either by clicking

on Help-> License Redemption Website from within the 7010 software, or by navigating directly to http://www.agilent.com/find/softwarelicense

Follow the instructions on the website. You will need your Software Entitlement Certificate as well as your Host ID as reported by the Agilent License Notifier:

The Host ID will appear when you hover your mouse cursor over the blue key icon in your system tray.

After completing the steps on the website, you will receive a .lic file via email. Save the .lic file to the computer being licensed.

From within the 7010 software, select "Install License(s)..." under the HelpFrom within the 7010 software, select Install License(s)... under the Help menu.

Browse to your saved .lic file. You should see a bubble in the system tray that says that the license was installed successfullythat says that the license was installed successfully.

Support

The hardware and software manuals are provided as .pdf documents with your 7010 software CD.y

For additional support, in North America call (800)227-9770 x3.

Outside North America contact your local distributorOutside North America, contact your local distributor.

new software features of the 7010 particle sizer.ppt · pdf file · 2016-08-30new...

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