New Generation of Confocal

Microscope

OLYMPUS 李承駿 易德明

Confocal Image

Gallery

Confocal !

Light source Light source

LASER

Mercury

Halogen

※ Pin hole technique

※ High-precision spectral system

Linear spectrum

400

800

700

600600

500

Prism

400

800

700

600600

500

Grating

Slit with 2 nm resolution

陰極板

陽極板

※ Detector photomultiplier (PMT)

共軛焦顯微鏡 一般顯微鏡

標本厚度 約 50um 數微米

平面解析度 0.55 λ /NA 0.61 λ / NA

Z 軸解析度 最好約 0.5um 很差

光學切片 可 不可

三維重組 可 不可

Confocal VS Epi-FL.

Phone Evolution

Simple is Beautiful

Inside the FV10i

暗房 共軛焦掃瞄器

雷射光源防震桌

顯微鏡

Step 1: Set Dye

Set sample choose dye

Step 2: Selecting Target

Auto Focus Map Image

Step 3: Image Acquire

Observation mode selection

Oil type or Water type?

Water type:

Built-in Incubator

N.A. 1.2, 60X water objective

Stable time-lapse imaging

Oil type:

N.A. 1.35, 60X oil objective

High-quality imaging

Thank you for your attention!!

Let’s give it a try!!