BPHOne of the most common ailments men face as they age is BPH—Benign Prostatic Hyperplasia—enlargement of the prostate caused by a rise in 5α-DHT levels as testosterone decreases and estrogen increases. Increased 5α-DHT causes the prostate to enlarge and compress the urethra, making urination di�cult. Reducing the e�ect of 5α-DHT can halt or reverse the advance of BPH, but current prescription medications often have undesirable side e�ects (see “Equol vs. Rx Enzyme Blockers”).

Introducing Prostizine™ with EquolEquol is the only known molecule that directly and exclusively binds 5α-DHT. It is one of the most studied iso�avinoids in the world, with proven bene�ts for the prostate.Studies prove that Equol is more e�ective than any other option in reducing symptoms associated with prostate enlargement—with no observed side e�ects. Prostizine™ with Equol also includes ingredients that have shown some success traditionally such as beta sitosterol, lycopene, and saw palmetto. This combination of ingredients makes Prostizine™ with Equol the safest, most e�ective next-generation prostate supplement available today.

E�ects of Equol & 5α-DHT on PSA Secretionby LNCap Prostate Cancer Cells in Vitro

PSA

(ng/

ml)

250

220

190

160

130

100

70

40

5α-DHT10nm

5α-DHT1nM

5α-DHT0.1nM

Equol 100nM+DHT 10nM

Equol 10nM+DHT 1nM

Equol 1nM+DHT 0.1nM

Serum 5α-DHT Levels / Prostate Weightin Equol-Treated Male Rats

serum 5α-DHT (pg/ml) prostate weight (mg)

100

control Equol control Equol

52

535

429

Equol has been extensively studied and researched with over 1,500 articles published in 150 peer-reviewed journals. In addition to promoting prostate health in men, Equol has been shown in animal and human studies to reduce body weight, reduce Leptin levels, support the thyroid, and reduce anxiety and depression.

CLINICALLY PROVEN:

Lephart et al. 2004, Nutr Metab.

These statements have not been evaluated by the FDA. This product is not intended to diagnose, treat, cure or prevent any disease.

Equol vs. Rx Enzyme BlockersThe most common treatments for BPH are enzyme blockers such as Proscar™ and Avodart™. These medications work by blocking the action of 5α-Reductase, an enzyme that normally converts

testosterone to 5α-DHT. By blocking this conversion, enzyme blockers reduce levels of circulating 5α-DHT, thereby inhibiting prostate growth.But 5α-Reductase (the blocked enzyme) has other positive functions in the body. Enzyme blockers

inhibit both the positive and the negative e�ects of the enzyme. This disruption of the normal enzymatic process is one of the key factors in the unwanted side e�ects that often accompany these medications.Equol binds directly with 5α-DHT, inhibiting prostate growth without interfering with normal enzymatic actions. As a result, Equol supports prostate health with no known side e�ects.

HH

Testosterone DHT

5α Reductase (enzyme)IncreasedProstateGrowth

Normal Enzyme Action

HH

Testosterone

5α Reductase (enzyme)Inhibits

Prostate Growth—with Side E�ects

Enzyme Blockers

HH

Testosterone DHT

5α Reductase (enzyme)Inhibits

Prostate Growth—NO Side E�ects

Equol (Binds 5α-DHT Exclusively)

Proscar™, Avodart™, etc.

Prostizine™

Study: Equol’s Other Positive E�ects in the BodyBody Weight

Thyroid (T3 Levels)Body Heat Production

(BAT, UCP-1 mRNA Levels)Insulin Levels

Anxiety & DepressionProstate Weight

Feeding (Food & Water Intake)Brain NPY Levels

Leptin LevelsGlucose Levels nsc nsc

Phyto-600(Equol)

Phyto-Free(Control)

NEW! FIRST GENERATION OF UNIVERSITY-DEVELOPED PROSTATESUPPORT PROVES SUPERIOR TO ALL EXISTING OPTIONS

ProsTizinewith EQUOL™

Supplement FactsServing Size: 1 capsulesServings Per Container: 60

Amount Per Serving % DV(R,S) Equol (patented & patent-pending) 6mg *Beta Sitosterol (Phytosterols) 150mg *Green Tea Ext (Camellia sinensis) 100mg * (90% Polyphenols / 50% EGCG)d-alpha tocopheryl succinate 100 IU *Lycopene 3mg *Saw Palmetto (95% pure extract) 100mg ** Daily Value Not Established

ProsTizinewith EQUOL™

Scienti�c Review: A Sample of MajorPeer-Reviewed Published Studies of Equol

MECHANISM OF HOW EQUOL WORKS(PROSTATE, WEIGHT CONTROL, BRAIN HEALTH)

1. Lund TD et al., Equol is a novel anti-androgen that inhibits prostate growth and hormone feedback, Biol Reprod, 2004, 70: 1188-1195

Discovery of equol’s ability to bind 5α-DHT and equol’s positive in�uences on prostate health for relieving BPH

2. KD Setchell et al., S-equol, a potent ligand for estrogen receptor beta, is the exclusive enantiomeric form of the soy iso�avone metabolite produced by human intestinal bacterial �ora, Am J Clin Nutr, 2005, 81:1072-1097

Discovery of equol’s pharmacokinetic properties (metabolism and half-life) in humans and binding and activating estrogen receptor beta (ER β) in vitro

3. T.E. Hedlund et al., Soy iso�avonoid equol modulates the growth of benign and malignant prostatic epithelial cells in vitro, Prostate, 2003, 54: 68-78.

Equol, in vitro, inhibits growth of benign and malignant prostatic epithelial cells

4. P.J. Magee et al., Equol: a comparison on the e�ects of the racemic compound with that of the puri�ed S-enantiomer on the growth, invasion, and DNA integrity of breast and prostate cells in vitro, Nutr. Cancer, 2006, 54: 232-242

While equol has been shown to have positive bene�ts in human prostate health, there is evidence that R-equol has strong antigenotoxic activity in human breast and prostate cells.

5. Tanaka M et al., Iso�avone supplements stimulated the production of serum equol and decreased serum dihydrotestosterone levels in healthy male volunteers, Prostate Cancer Prostatic Diseases, 12: 247-252, 2009

Iso�avone supplementation increase circulating equol levels in the bloodstream and then signi�cantly decreased serum (5α-DHT) levels

6. Blake C et al., Prenatal exposure to equol decreases body weight and depressive-like behaviors in male and female o�spring, Curr Topics Nutr Res, 2010, 8: 69-77

Administration of equol at di�erent doses prenatally resulted in signi�cant reductions in body weights of the mother and o�spring and decreased depression in o�spring before puberty

7. Henry-Vitrac C et al., Soy Iso�avones as potential inhibitors of Alzheimer Beta-amyloid �bril aggrega-tion in vitro, Food Res International, 2010, 43: 2176-2178

Equol > genistein inhibits amyoids aggregation in vitro

8. Ma Y et al., Dietary genistein and equol (4’, 7 iso�avandiol) reduce oxidative stress and protect rats against focal cerebral ischemia, Am J Physiol Regul Integr Comp Physiol, 2010, 299: R871-R877

Equol > genistein inhibits oxidative stress and focal cerebral ischemia

SAFETY/TOXICOLOGY9. V Selavraj et al., Estrogenicity of the Iso�avone Metabolite Equol on Reproductive and Non-reproductive Organs in Mice, Biol Reprod, 2004, 71:966-972.

10. CE Wood et al., E�ect of High-Dose Soy Iso�avones and Equol on Reproductive Tissues in Female Cynomolgus Monkeys, Bio Reprod, 2006, 75:477-486

ANTIOXIDANT AND PROTECTIVE PROPERTIES11. JH Mitchell et al., Antioxidant E�cacy of Phytoestrogens in Chemical and Biological Model Systems, Arch Biochem Biophys, 360:142-148, 1998.

12. A Arora, MG Nair and GM Strasburg, Antioxidant Activities of Iso�avones and Their Biological Metabolites in a Liposomal System, Arch Biochem Biophys, 356:133-141, 1998.

13. CE Rufer and SE Kulling, Antioxidant Activity of Iso�avones and Their Major Metabolites Using Di�erent in Vitro Assays, J Agric Food Chemistry, 54:2926-2931, 2006.

14. JE Chung et al., Antioxidant E�ects of Equol on Bovine Aortic Endothelial Cells, Biochem Biophys Research Comm., 375:420-424, 2008.

15. M Kamyama et al., E�ects of Equol on Oxidized Low-Density Lipoprotein-Induced Apoptosis in Endothelial Cells, J Atherosclerosis Thrombosis, 16:239-249, 2009.

16. C Cheng et al., The Soybean Iso�avonoid Equol Blocks Ritonavir-induced Endothelial Dysfunction in Porcine Pulmonary Arties and Human Pulmonary Artery Endothelial Cells, Journal Nutrition, 140:12-17, 2010.

17. Munoz Y et al., Equol is more active than soy iso�avone itself to compete for binding to thrombox-ane A(2) receptor in human platelets, Thrombosis Res, 123: 740-744, 2009

18. Kamiyama M et al., E�ects of Equol on Oxidized low-density lipoprotein-induced apoptosis in endothelial cells, J Atherosclerosis Thrombosis, 16: 239-249, 2009

BACKGROUND AND SOURCES OF EQUOL 19. N Hounsome et al., Changes in Antioxidant Compounds in White Cabbage During Winter Storage, Postharvest Biology & Technology, 52:173-179, 2009.

Equol found in plants, white cabbage with storage.

20. K Setchell et al., The Clinical Importance of the Metabolite Equol- A Clue to the E�ectiveness of Soy and Its Iso�avones. J Nutr 2002, 132;3577-3584.

Comprehensive review of equol, background, synthesis, metabolism and actions

21. J-P Yuan et al., Metabolism of dietary soy iso�avones to equol by human intestinal micro�ora-implications for health, Mol Nutr Food Res, 2007, 51:765-781.

Comprehensive review of equol, background, synthesis, metabolism and actions

22. A Hoikkala et al., High levels of equol in organic skimmed Finnish cow milk. Mole Nutr Food Res 2007, 51: 782-786

Evidence of equol in cow’s milk via consumption of plant sources

23. Mustonen EA, Equol in milk of dairy cows is derived from forage legumes such as red clover, British J Nutr, 102: 1552-1556, 2009

Evidence of equol in cow’s milk via consumption of plant sources

24. Walsh KR, Transport and Metabolism of Equol by Caco-2 Human Intestional Cells, J Agr Food Chem, 57: 18:8297-8302

In vitro, study of transport of equol con�rming its half-half and metabolism

25. Setchell KDR and Clerici C, Equol: History, Chemistry and Formation, J Nutr, 140: 1355S-1362S

Comprehensive review of equol, background, synthesis, metabolism and actions

26. Brown NM, et al., The chemopreventive action of equol enantiomers in a chemically induced animal model of breast cancer, Carcinogenesis, 31: 886-893, 2010

Importance of R-equol (greater than S-equol) in chemoprevention in an animal breast cancer model

27. Ishimi Y, Dietary equol and bone metabolism in postmenopausal Japanese Women and osteoporotic mice, J Nutr, 2010, 140: 1373S-1376S

Equol protective e�ects against osteoprosis

28. Sehmisch S et al., E�ects of Iso�avones equol and genistein on bone quality in a rat osteopenia model, Phytotherapy Res, 24: S168-S-174

Equol protective e�ects against osteoprosis (equol > genistein)