neon cell protocols - thermo fisher scientific · neon™ transfection system cell protocols for...
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Neon™transfection system
cell protocols
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PBMCThis protocol requires Bu�er T for Primary Cells.
Cell informationCell type/Description PBMC (peripheral blood mononuclear cell)/Primary cell
Characteristics/Species Suspension/Human
Tissue origin Blood
Media RPMI 1640 with L-glutamine and 10% FBS
Morphology Round single cell
Doubling time NA
Subculturing Medium renewal every 2 to 3 days
Culture conditions Temperature: 37°C; atmosphere: air 95%/carbon dioxide (CO2) 5%
Electroporation parametersPulse
voltage (v)Pulse
width (ms)Pulse
numberCell density
(cells/mL)Transfection
ViabilityTip
type
2,150 20 1 2 x 107 23% 95% 10 µL
Peripheral blood mononuclear cells (PBMCs) were transfected using the Neon™ transfection system and 1.0 µg of a plasmid encoding EGFP. 24 hours posttransfection, the cells were analyzed by light (A) and fluorescence microscopy (B).
A B