nematode extraction methods: root extraction techniques
DESCRIPTION
Nematode Extraction Methods: Root Extraction Techniques. Kavitha Govindasamy Jimmy R.Rich Maria L .Mendes. A Plant-Parasitic Nematode. Sampling for Nematodes. Sampling and extracting for nematodes serves two purposes: Diagnosis a current problem Predict a future problem. - PowerPoint PPT PresentationTRANSCRIPT
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Nematode Extraction Methods:Root Extraction Techniques
Kavitha GovindasamyJimmy R.RichMaria L .Mendes
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A Plant-Parasitic Nematode
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Sampling for Nematodes
Sampling and extracting for nematodes serves two purposes:
Diagnosis a current problem Predict a future problem
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Objectives of sampling and extracting nematodes
Identify the nematode Population estimation Diagnose a nematode disease Make management decisions Regulatory purposes Research and surveys
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Some Common Nematode Extraction Methods
Root Samples Jar incubation and sieving Blending and sieving Soil samples Baermann funnel Modified Baermann funnel Foliar samples Punching and incubating Scissors technique
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Root Extraction Techniques:Jar Method
Principle
Nematodes are extracted based on their active migration from roots
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Materials required
Glass jars and caps 325 or finer mesh sieve Labels and rubber bands Dark chamber
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Sample ready for processing
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Wash the roots
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Damp dry the roots
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Weigh the damp-dried roots
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Place roots in jars, add water
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Place jars in tray
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Store in the dark for 72 hours
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Following incubation, remove from storage and add water
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Collect nematodes on sieve
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Rinse nematodes from sieve
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Nematode samples ready for observation
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Advantages and Limitations
Advantages Extracts migratory endoparasitic nematodes Few materials needed Simple procedure to follow
Limitations Not useful for ectoparasitic nematodes Lack of aeration can be a problem Sieves are needed to concentrate nematodes
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Root Extraction Techniques:Blending and Sieving
Principle
Nematodes are released from roots due to maceration.
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Materials required
Blender 40 and 325 or 500 mesh sieves Pan or bowl Wire mesh Filter paper Squeeze bottle Weighing balance Scissors
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Wash roots well
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Cut roots
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Damp dry the roots
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Weigh roots
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Place roots into blender, cover roots with water
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Blend at low speed for 10-20 seconds
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Pour the roots into 325 or finer mesh sieve
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Set up a Baermann tray
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Place filter paper on the Baermann tray
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Add water to Baermann tray
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Transfer the blended roots into the filter paper
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Incubate for 24 hours
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Remove roots from the pan
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Pour the water in the dish through a 325 or fine mesh sieve
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Concentrate nematodes for observation
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Advantages and Limitations
Advantages Fast method to extract nematodes from roots Foliar nematodes can also be easily extracted
Limitations Chemicals released from the roots can be injurious to nematodes Blending for long time may damage nematodes
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Acknowledgments
Janete Brito, Department of Plant Industry, Gainesville, Florida
Frank Woods, Nematode Assay Lab, University of Florida, Gainesville, Florida
Joey Orajay, Entomology and Nematology Department, University of Florida, Gainesville, FL
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