N-Process Improvement of N mineral nutrition

Grassland Agro Conference R&D TAI Roullier Group

JM Garcia-Mina (JGM v1 2014)

• N fertilization – Main Topics

– N root uptake

– N fertilizer metabolic efficiency within the plant

– N losses: Efficiency and environmental problems

– Soil N mobilization

N fertilization Problems – N-Process solutions

– N root uptake – NP1 PMA swc action

– N fertilizer metabolic efficiency within the plant – NP1 PMA swc syn. actions

– N losses: Efficiency and environmental problems – NP2 NSTA Action NAU nutrition

– Soil N immobilization – NP3 SEA 1 Action

N-Process action on soil-plant system

NS TA - Matrix

In granule

SOIL

NUTRIENTS

Root

PMA SWC

N Fert - units –NAU effect

Gradual N release

Soil Microbial Activity Soil Enzymatic activities (mainly

Soil Proteases and ureases )

Rhizosphere

N transformation N root uptake

SEA 1

A vailable fractions of nutrients

in soil, mainly inorganic nitrogen

N leaching

N water - soil contamination

-

NH3 volatilization

• NP1 (PMA SWC) is a phytoregulatory complex, composed of a specific family of N-heterocyclic molecules with auxin-CKs activity, selected and purified from several seaweed species.

This Phytoregulatory complex has the ability to:

• (i) Increase in NR // GS GOGAT Activities

• (ii) Increase in Z-Pool transformation of S and C

• This effect increases N transformation in proteins and aa (in shoot and root)

NP2. NS TA Process is an organo-mineral matrix formed by calcium sulphate X-hydrated and

calcium sulphate tetra-ureate, where calcium atoms are complexed by humic molecules.

Pre-granulator Reaction of N forms Matrix formation

Stabilization

Process

Specificity HA

Urée SA 21

Fusion

Gran Drum

Screen

Mill Dryer

Screen

Sécheur

Cooler

Conditioning-Coating

NS TA

N-PROCESS

Homogeneity

Resistance

No auto-explosion Risk

NS TA Matrix

Gradual release

Technical Requirements

(H2O)

XO

M

PCA

M

MPCA

M

MPCA

M

MPCA

(H2O)

XO

M

(H2O)

XO

M

(H2O)

XO

M

(H2O)

XO

M

(H2O)

XO

M

(H2O)

XO

M

ESTRUCTURA

CRISTALINA

MATRIZ ORGÁNICA

HIDROFÓBICA

Main Effects:

The presence of the NSTA Matrix in the fertilizer granule has two complementary effects:

1. A Physiological effect by improving N fertilization efficiency through the stabilization of the gradual

simultaneous presence in the soil solution of nitrate (coming from ammonium soil nitrification), ammonium

and urea: NAU effect

2. Environmental and nutritional effects derived from the control of both N soil leaching and ammonium

volatilization.

Publications

Wheat

Height

cm Tillering

DW Shoot

g/plant

DW Root

g/plant

DW

plant

NO3- 48 b 4.95 a 1.87 a 0.32 a 2.19 a

NA 44.4 a 5.27 a 1.98 a 0.44 b 2.41 ab

NAU 45.2 ab 6.16 b 2.22 b 0.35 a 2.57 b

Same N dose NAU contains less NO3- (1/3)

than NO3- treatment

NAU vs NA and NO3-

NAU effect on the plant hormonal balance.

NAU effect on the plant hormonal balance.

NAU effect on the plant hormonal balance.

• CYTOKININS

Z, tZR (MORE ACTIVE FORMS), IPR

cZR, (LESS ACTIVE or INACTIVE FORMS)

• IAA

• ABA

100µM

5mM

■ NO3-/ NH4

+ U

IAA BIOSYNTHESIS IN SHOOTS 100µM

5mM

100µM

5mM ABA BIOSYNTHESIS IN SHOOTS

Enhance SHOOTS development

CONCLUSIONS

NS TA

Gradual Release ---- Reduction Losses

San Francisco et al., 2012

1 2 3 4

5 6

7 8

9 10

11

0,00

10,00

20,00

30,00

40,00

50,00

60,00

70,00

80,00

90,00

100,00

% d

e N

itró

gen

o

Lavados

NProcess 29N-verde

NProcess 29N

NProcess 214-10N verde

NProcess 214-10N

Nitrato de amonio

Urea

Reduction in ammonium volatilization

-18

-16

-14

-12

-10

-8

-6

-4-2

0

7 8 9 11 14

N-PROCESS

urée

Organic sites in NSTA matrix-

urea Intéraction

R&D TAI and CERA

Study

NP3. SEA 1. This compound is a humate-auxin precursor complex with the ability to

activate soil-enzyme activities, and specifically the protease and urease soil

activities. This action permits the mineralization of nitrogen from proteins-residues and amidic-residues (in general,

organic nitrogen) in soil humic substances, thus increasing N soil availability for plants.

HHA-AxP

Soil Organic N Mobilization

Rhizosphere - Romola Soil

N-Process action on soil-plant system

NS TA - Matrix

In granule

SOIL

NUTRIENTS

Root

PMA SWC

N Fert - units –NAU effect

Gradual N release

Soil Microbial Activity Soil Enzymatic activities (mainly

Soil Proteases and ureases )

Rhizosphere

N transformation N root uptake

SEA 1

A vailable fractions of nutrients

in soil, mainly inorganic nitrogen

N leaching

N water - soil contamination

-

NH3 volatilization

MPPA Duo

Grassland Agro Conference R&D TAI Roullier Group

JM Garcia-Mina (JGM v1 2014)

PLANTA

FERTILIZANTE SUELO

D

MPPA Duo

A soil-plant-fertilizer catalyzer

D

Increase Yield and plant-soil Quality

MPPA-Nutrients

XCK Complex

Binding sites

Ca : Cation (Fe+++,Al+++ Acid Soils; Ca++ Calcareous Soils

II. D-N

- NH4+ - Urée

III. D-K

III. D-Oligo

Increase Yield and plant-soil Quality

MPPA-Nutrients

XCK Complex

Effect on plant development

Trat. Spad

height

(cm)

Nº of

leaves

Leaf d w

(g)

Root d w

(g)

Stem d w

(g) Plant d w

(g)

C 41,4 17 56,0 25,7 10,7 6,6 43.1

AIA -6 46,1 19,3 77,0* 37,0* 13,6 9,7* 60.2*

XCK 17 -3

43,0 14,4* 38,2* 13,2* 5,0* 3,2* 21.3*

XCK 17 -6

43,4 18,5 81,0* 34,2 13,6 8,8 56.5

XCK 17 -9

42,9 20,1* 79,7* 37,0* 13,0 10,2* 60.3*

* LSD for p< 0.05.