multispecies cytograms
TRANSCRIPT
V2.2.06MS 9/1/00
Cat WBC
This area includes nonlysed MN’s that are included in the MN count. Samples should be run at room temp to minimize nonlysis.
Eos’s average 2%-12% but may be as high as 35%
Eos’s not counted here
Eos’s are counted in the retic channel (even without ordering a retic). Retic reagent must be available on the analyzer at all times when running cats.
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Cat with Large Platelets
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Cat with Platelet ClumpsPlatelet clumps appear here
Platelet clump count located here
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Cat HGB, Platelet and RBC
High MPV (cat MPV value can be up to 25)
Platelets can be larger than RBC’s
Platelet cytogram is very diffuse due to platelet activation.
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Cat Retic
Eos’s are counted in the retic channel.
Three times as much sample is pushed through the flowcell when running cat.
Throughput for CBC/Diff on cat is 74 samples/hour.
Low Retic count normal for cat
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Cattle WBC
Elongated MN population
Cattle can have 0-25% EOS’s
Mono
LUC
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Cattle HGB, Platelet, and RBC
Low MCV
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Cattle Retic
Cattle have a very low retic count or no retics at all therefore retics do not need to be run on cattle.
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Dog WBC
Mono’s about 1% higher on ADVIA 120
Baso positioned lower
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Dog HGB, RBC and Platelet
Platelets look very diffuse - activated
Large platelets common
MPV can be 15ƒL
Platelet activation may be a function of the draw technique
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Dog Platelet (with less platelet activation)
Platelets less diffuse
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Dog Retic
%Retic low on dogs (but can be 5%)
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Goat WBC
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Goat HGB, Platelet and RBC
Goats can have 1,000,000 platelets
RBC count very high and MCV very low
2D platelet analysis used to obtain RBC count
Lower threshold set at zero
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Goat Retic
Low retic results
No Retic parameters
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Guinea Pig WBC
Diffuse Baso
PMN population slants down
Lymph
LUC
Mono
Guinea Pigs have manyLUC’s
Lymphs contain Kurloff inclusions
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Guinea Pig HGB, Platelet and RBC
Small Platelets
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Guinea Pig Retic
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Horse WBC
Eos’s are located out to the side and are giant in size
Mono’s
LUC’s
Unlysed MN - can warm the blood but non-lysis may not be eliminated
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Horse HGB, Platelet and RBC
Low MCV
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Horse Retic
Horses do not have retics
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Monkey-Cyno WBC
Perox cytogram looks like a weak staining human
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Monkey-Cyno HGB, Platelet and RBC
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Monkey-Cyno Retic
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Monkey-Rhesus WBC
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Monkey-Rhesus HGB, Platelet and RBC
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Monkey-Rhesus Retic
<1% Retics normal
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Mouse WBC
Mice do not have many mono’s
Inverted Diff
Lipids common for mice
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Mouse with Platelet Clumps
Platelet clumps
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Mouse HGB, Platelet and RBC
RBC
MCV
Platelet
MPV
Platelet count about 5% higher (on samples with platelet clumps) than H1E due to difference in mixing between analyzers
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Mouse Retic
Common pattern
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Pig WBC
LUC’s
Mono’s
Neutrophils sometimes weak stainers
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Pig HGB, Platelet and RBC
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Pig Retic
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Rabbit WBCMany Baso’s
Very little noise
Lymph threshold is low due to small size of lymphs
Baso’s can equal 4-10%
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Rabbit HGB, Platelet and RBCSmall Platelets
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Rabbit Retic
Rabbits can have 5%-6% Retics
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Rat WBC
Inverted diff
Platelet clumps common (see next Run Screen)
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Rat-LE with Platelet Clumps
Platelet Clumps common due to draw technique
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Rat-LE HGB, Platelet and RBC
Platelet count usually higher on the ADVIA 120 (for samples with platelet clumps than H1E due to differences in mixing between analyzers
Platelet Clumps common
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Rat Retic
Rat Retic >2%
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Sheep WBC
LUC’s
Mono’s
Lymphs
Inverted Diff
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Sheep HGB, Platelet and RBC
Volume Threshold set to zero
RBC
MCV
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Sheep Retic
Low %Retic