mr. martinez. 1 onion root lab: modeling the phases of the cell cycle
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Mr. Martinez.
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Onion Root Lab: Modeling the Phases of the Cell Cycle
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The Cell Cycle
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The Cell Cycle is the series of events that take place in a cell leading to its division and duplication (replication) .
The Cell Cycle has 3 Stages: Interphase, Mitosis, and Cytokinesis.
Mitosis is a type of cell division that results in two daughter cells each having the same number and kind of chromosomes as the parent nucleus
Mitosis has 4 phases: Prophase, Metaphase, Anaphase, and Telophase
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Onion, a garden plant of the lily family, closely related to the leek, garlic, chive, and shallot. The bulb of the onion plant, also called onion, is widely used as a seasoning and is eaten as a vegetable. Although onions have little food value, they impart a desirable flavor to stews, soups, hamburgers, and other dishes.
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Mitosis in Root Tip
In a growing plant root, the cells at the tip of the root are constantly dividing to allow the root to grow.
Because each cell divides independently of the others, a root tip contains cells at different stages of the cell cycle.
This makes a root tip an excellent tissue to study the stages of cell division
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Stages of mitosis in onion root tip cells
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Experiment
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Materials
Prepared Onion Root Tip Slides Microscope
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Observe your preparation under the low power (X10) of a microscope
Search the slide to find cells in various stages of cell division, once you have located cells in division, change to high power (X40) & try to observe several stages of division.
Record the number of cells in each stage. Record your data in the table Calculate the percentage of cells in each phase and record
in the table
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Mitotic index
A measure for the proliferation status of a cell population.
It is defined as the ratio between the number of cells in mitosis and the total number of cells.
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Animation
Lab: http://www.youtube.com/watch?v=mUBlZ1VNReQ
Video http://www.youtube.com/watch?v=djXhEJUldNc
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Interphase Prophase
Metaphase Anaphase
Telophase