mouse nomenclature - washington university...
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Mouse NomenclatureAdvanced Genetics
2/28/19
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Wild type…• Not exactly wild in traditional sense• Inbred defined as 20 consecutive generations of sib mating• C57BL/6J• C57BL = parent strain• 6 = line• J = Jackson Lab code
• Lab codes designate where crossing occurred• J – Jackson• Mcw – Medical College of Wisconsin• Kyo – Kyoto University• Mit – MIT
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Crossing Strains
• Progeny of parental strains designated as F1Hybrid• Intercrossing two F1 hybrids gives rise
to F2 generation• D2B6F1
• DBA/2 mother and C57BL/6J father• D2B6F2
• Offspring of a D2B6F1 cross
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Genes and Loci
• First letter capitalized• Italicized• Example• Apc = adenomatosis polyposis coli
• Mutant allele name may be placeholder for unknown gene • Leprdb used to be Dbdb
• Loci: D8Mit17 = 17th locus on mouse chromosome 8 identified at MIT
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Alleles
• Named by phenotype• Superscripted • Italicized• Dominant = first letter capitalized• Recessive = all lowercase• Examples:• Apc Min = dominant, multiple intestinal neoplasia allele of adenomatosis
polyposis coli gene• Leprdb = recessive, diabetes allele of leptin receptor gene
https://www.genome.gov/10005831/2002-release-the-mouse-genome-and-the-measure-of-man/
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Genotypes
• Includes: genetic background – geneallele/ lab designation• Notation for spontaneous mutations: • MRL/MpJ-Faslpr/J
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Targeted Knock-out, Knock-in, or Floxedmutations• Modified genes superscripted with “tm” for targeted mutations
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Transgenic Strains
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Proteins
• All capitol letters• No italics• Examples• LEPR• APC
https://www.genengnews.com/news/crispr-without-the-chop-reverses-genetic-obesity-in-mice/
leprdb/dblepr+/+
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Review of nomenclature across models
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Resources
• https://www.jax.org/jax-mice-and-services• http://www.informatics.jax.org/mgihome/nomen/• http://www.informatics.jax.org/