monthly report: surveillance project sb4008 ifng tests for

Monthly Report:Surveillance ProjectSB4008 IFNg tests forbovine tuberculosis

(TC0651 and TC0751)

Number

Report Period

Issued 10 February 2019

Operational notes on terminology, definitions, re-test and re-samples testoutcomes:

• “Samples” refers to an individual tube of blood taken from an individual animal which issubsequently submitted for testing. It is generally assumed that the number of samples andthe number of animals are analogous as any duplicate sample blood tube from the sameanimal will not be tested. However, minor discrepancies may occur where the same animal istested more than once due to a request for a resample or the animal is tested twice underdifferent categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).

• “Submissions” refers to an individual batch or set of samples received for testing.Submissions may comprise 1 or more samples (for example, if an entire herd is being tested itis usually sent as 1 submission). It is generally assumed that the number of submissions andthe number of herds are analogous.

• Retests are samples where the first ELISA assay fails and the same sample is retested on anew ELISA plate. Each sample can be retested only once. A retest is not a reportable testoutcome.

• Resamples are where a sample has been retested and failed a second time, so that the labrequests a new sample. This is a reportable test outcome.

• Rejects are samples that are not tested by the lab for one of the following reasons: bloodcollected into wrong type of vacutainer, samples that have not been maintained at theappropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, bloodthat is extensively clotted (small clots are OK), samples received after 4pm on the day aftersample collection. Such samples are reported separately in the tables and in figure 5.

• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure ofthe quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) mayindicate compromised blood quality as a result of collection/transportation conditions or duethe animal having an unusually low/suppressed cellular immune response.

• NEG (no-antigen control) is a sample negative control which provides a measure of thebackground antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)may indicate a laboratory procedures problem (normally resolved during re-test) or that ananimal has unusually high background levels of IFN-g.

Submission Reason Explanation Mandatory?

Possible Herd Slaughter(PAR-HERD-S)

Parallel interferon-gamma blood testing of skintest negative cattle to inform whole or partialherd slaughters decisions

YES, if APHA arecontemplating a herdslaughter

Persistent TB breakdowns(OTF status withdrawn)(PAR-P-CONF) N.B.PAR-PERSIS from 1st April2017

Parallel blood testing of skin test-negative cattlein persistently infected herds that have failed toresolve by repeated short-interval skin testingand fulfill a minimum of biosecurity standards.

NO (APHA discretion)

Parallel - Low Incidence(PAR-LOW-IN) N.B.PAR-NEW-IN from 1st April2017

Parallel blood testing to maximise theprobability of removing all infected cattle in anew herd breakdown as soon as possible afterconfirmation of TB.

YES in Area of LowIncidence

Rapid Testing of twice IR's(PAR-RAPID)

Parallel blood testing of two-times IRs identifiedunder the severe interpretation of the skin testused in Wales.

YES (Wales only)

PAR-CUL-N

Parallel blood testing to maximise theprobability of removing all infected cattle in anew breakdown as soon as possible afterconfirmation of TB where herd is situated inbadger control areas of England that havecompleted at least 2 effective culls.

YES (in eligible badgercontrol areas of England)

PAR-CUL-P

Parallel blood testing to maximise theprobability of removing all infected cattle in apersistent confirmed breakdown where herd issituated within badger control areas of Englandthat have completed at least 2 effective culls


Parallel Other(PAR-OTHER)

Other parallel blood testing not covered in anyof the other scenarios NO (APHA discretion)

'NSR' Herds (SER-NSR)

Modified serial blood testing of individual skintest reactors and/or IRs in unconfirmed TBbreakdown herds to clarify their infection statuswhere there is evidence of non-specificsensitisation to bovine tuberculin (the“non-specific reactor” procedure)


Suspected Fraud(SER-FRAUD)

Modified serial blood test of suspectedfraudulent reactors to the skin test (animals withabnormal skin swellings), in confirmed orunconfirmed TB incidents.

NO (APHA discretion tofirm up or rule out anysuspicion of fraud andsupport anyinvestigations)

Serial Other(SER-OTHER) Serial test – other reasons NO (APHA Discretion)

SER-FLEXI

Extended blood test to provide flexible testreadout in a confirmed M. bovis-infected herdwhere Johne's (M. a. paratuberculosis) infectionor vaccination is suspected to be interferringwith M. bovis infection detection


Test Criteria

NumsamplesJanuary2019

% Total 2019 %

Submissions 289 289

Samples Total (%) 27513 100.00 % 27513 100.00 %

England 20585 74.82 % 20585 74.82 %

Wales 6928 25.18 % 6928 25.18 %

Scotland

Parallel Tests PAR-CUL-N 2848 10.35 % 2848 10.35 %

PAR-CUL-P 2527 9.18 % 2527 9.18 %

PAR-HERD-S 634 2.30 % 634 2.30 %

PAR-NEW-IN 13956 50.73 % 13956 50.73 %

PAR-OTHER 1605 5.83 % 1605 5.83 %

PAR-PERSIS 5313 19.31 % 5313 19.31 %

PAR-RAPID 173 0.63 % 173 0.63 %

Total(% of all samples) 27056 98.34 % 27056 98.34 %

Serial/Extended Tests SER-FLEXI 450 1.64 % 450 1.64 %

SER-FRAUD 0 0.00 %

SER-NSR 7 0.03 % 7 0.03 %

SER-OTHER 0 0.00 %

Total(% of all samples) 457 1.66 % 457 1.66 %

Total 27513 100.00 % 27513 100.00 %

Retests Total (% of all samples) 1937 7.04 % 1937 7.04 %

England 1526 5.55 % 1526 5.55 %

Wales 411 1.49 % 411 1.49 %

Scotland

Resamples Total (% of all samples) 1657 6.02 % 1657 6.02 %

England 1335 4.85 % 1335 4.85 %

Wales 322 1.17 % 322 1.17 %

Scotland

Rejects Total (% of all samples) 974 3.54 % 974 3.54 %

England 860 3.13 % 860 3.13 %

Wales 114 0.41 % 114 0.41 %

Scotland 0 0.00 %

Table 1. Monthly Totals for January 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother

PAR-CUL-N

PAR-CUL-PPAR-HERD-S

PAR-NEW-IN

PAR-OTHER

PAR-PERSIS

PAR-RAPIDSER-FLEXI

SER-NSR

Breakdown ofReason forsubmission

PAR-CUL-N 2848 10.4 %

PAR-CUL-P 2527 9.2 %

PAR-HERD-S 634 2.3 %

PAR-NEW-IN 13956 50.7 %

PAR-OTHER 1605 5.8 %

PAR-PERSIS 5313 19.3 %

PAR-RAPID 173 0.6 %

SER-FLEXI 450 1.6 %

SER-NSR 7 0.0 %

Total 27513 100.00 %

Figure 1. Number of samples submitted in each category during January 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother

0

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Gwynedd

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Wiltshire

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Shropshire

Oxon

Notts

Northants

Lincs

Leics & Rut

Lancashire

Heref

Hampshire

Gloucs

E Sussex

Dorset

Devon

Derbyshire

Cumbria

Corn & Scilly

Cheshire

Cambs

Bucks

Berks

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Wales

England

SER-NSR

SER-FLEXI

PAR-RAPID

PAR-PERSIS

PAR-OTHER

PAR-NEW-IN

PAR-HERD-S

PAR-CUL-P

PAR-CUL-N

Breakdown ofSamples

submitted byCounty

Figure 2. Number of samples submitted in each category by County during January 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallelother, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serialpotential fraud, SER-OTHER: serial other

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S Yorks

Somerset

Shropshire

Oxon

Notts

Northants

Lincs

Leics & Rut

Lancashire

Heref

Hampshire

Gloucs

E Sussex

Dorset

Devon

Derbyshire

Cumbria

Corn & Scilly

Cheshire

Cambs

Bucks

Berks

Avon

Wales

England

SER-NSR SER-FLEXI PAR-RAPID PAR-PERSIS PAR-OTHER PAR-NEW-IN PAR-HERD-S PAR-CUL-P

PAR-CUL-N

Breakdownof Samplessubmittedby County

Figure 3. Number of samples submitted in each category by County during 2019

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrentJohne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Table 2a. Summary by County for January 2019

Country County SubmissionReasons*

No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject

n % of total n % n % n %

England - HRA Avon PAR-PERSIS 2 200 0.73 % 12 6.00 % 169 84.50 % 20 10.00 % 17 8.50 % 2 1.00 %

Corn & Scilly PAR-CUL-N 7 697 2.53 % 27 3.87 % 638 91.54 % 41 5.88 % 32 4.59 % 0 0.00 %

PAR-CUL-P 2 989 3.59 % 17 1.72 % 873 88.27 % 119 12.03 % 97 9.81 % 2 0.20 %

PAR-PERSIS 2 106 0.39 % 1 0.94 % 9 8.49 % 1 0.94 % 1 0.94 % 95 89.62 %

SER-FLEXI 2 15 0.05 % 0 0.00 % 15 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Devon PAR-CUL-N 8 810 2.94 % 65 8.02 % 694 85.68 % 59 7.28 % 50 6.17 % 1 0.12 %

PAR-CUL-P 2 441 1.60 % 18 4.08 % 401 90.93 % 29 6.58 % 22 4.99 % 0 0.00 %

PAR-NEW-IN 1 344 1.25 % 67 19.48 % 242 70.35 % 42 12.21 % 35 10.17 % 0 0.00 %

PAR-PERSIS 3 396 1.44 % 25 6.31 % 330 83.33 % 42 10.61 % 41 10.35 % 0 0.00 %

SER-FLEXI 2 62 0.23 % 6 9.68 % 25 40.32 % 0 0.00 % 0 0.00 % 31 50.00 %

Dorset PAR-CUL-N 5 378 1.37 % 23 6.08 % 314 83.07 % 41 10.85 % 40 10.58 % 1 0.26 %

PAR-CUL-P 2 869 3.16 % 38 4.37 % 795 91.48 % 37 4.26 % 36 4.14 % 0 0.00 %

Gloucs PAR-CUL-N 6 620 2.25 % 30 4.84 % 446 71.94 % 53 8.55 % 44 7.10 % 100 16.13 %

Heref PAR-CUL-N 2 343 1.25 % 15 4.37 % 311 90.67 % 18 5.25 % 17 4.96 % 0 0.00 %

Shropshire PAR-OTHER 2 454 1.65 % 24 5.29 % 392 86.34 % 44 9.69 % 38 8.37 % 0 0.00 %

PAR-PERSIS 1 72 0.26 % 2 2.78 % 56 77.78 % 15 20.83 % 14 19.44 % 0 0.00 %

Somerset PAR-CUL-P 1 228 0.83 % 21 9.21 % 196 85.96 % 17 7.46 % 9 3.95 % 2 0.88 %

PAR-OTHER 2 238 0.87 % 34 14.29 % 155 65.13 % 8 3.36 % 8 3.36 % 41 17.23 %

Wiltshire PAR-OTHER 1 36 0.13 % 1 2.78 % 33 91.67 % 4 11.11 % 2 5.56 % 0 0.00 %

SER-FLEXI 1 2 0.01 % 0 0.00 % 1 50.00 % 1 50.00 % 1 50.00 % 0 0.00 %

England - Edge Berks PAR-NEW-IN 7 1,009 3.67 % 23 2.28 % 921 91.28 % 72 7.14 % 65 6.44 % 0 0.00 %

PAR-PERSIS 1 92 0.33 % 4 4.35 % 87 94.57 % 2 2.17 % 1 1.09 % 0 0.00 %

Bucks PAR-NEW-IN 1 153 0.56 % 12 7.84 % 138 90.20 % 7 4.58 % 3 1.96 % 0 0.00 %

Cheshire PAR-NEW-IN 23 4,679 17.01 % 108 2.31 % 4172 89.16 % 294 6.28 % 249 5.32 % 150 3.21 %

PAR-PERSIS 1 13 0.05 % 1 7.69 % 12 92.31 % 0 0.00 % 0 0.00 % 0 0.00 %

Derbyshire PAR-NEW-IN 18 2,797 10.17 % 85 3.04 % 2365 84.55 % 154 5.51 % 134 4.79 % 213 7.62 %

E Sussex PAR-NEW-IN 5 807 2.93 % 46 5.70 % 719 89.10 % 30 3.72 % 29 3.59 % 13 1.61 %

Hampshire PAR-NEW-IN 6 55 0.20 % 1 1.82 % 51 92.73 % 3 5.45 % 3 5.45 % 0 0.00 %

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)

Table 2a. Summary by County for January 2019 Cont.




SER-NSR 1 7 0.03 % 0 0.00 % 7 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Leics & Rut PAR-NEW-IN 5 475 1.73 % 22 4.63 % 316 66.53 % 54 11.37 % 50 10.53 % 87 18.32 %

Northants PAR-NEW-IN 1 6 0.02 % 0 0.00 % 5 83.33 % 2 33.33 % 1 16.67 % 0 0.00 %

Notts PAR-NEW-IN 3 377 1.37 % 15 3.98 % 259 68.70 % 44 11.67 % 40 10.61 % 63 16.71 %

Oxon PAR-NEW-IN 17 1,858 6.75 % 101 5.44 % 1570 84.50 % 198 10.66 % 187 10.06 % 0 0.00 %

Warks PAR-NEW-IN 10 546 1.98 % 24 4.40 % 428 78.39 % 38 6.96 % 35 6.41 % 59 10.81 %

PAR-PERSIS 2 157 0.57 % 9 5.73 % 137 87.26 % 12 7.64 % 11 7.01 % 0 0.00 %

England - LRA Cambs PAR-NEW-IN 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %

Cumbria PAR-NEW-IN 1 3 0.01 % 0 0.00 % 2 66.67 % 1 33.33 % 1 33.33 % 0 0.00 %

Lancashire PAR-NEW-IN 1 22 0.08 % 0 0.00 % 21 95.45 % 1 4.55 % 1 4.55 % 0 0.00 %

Lincs PAR-NEW-IN 3 60 0.22 % 8 13.33 % 41 68.33 % 11 18.33 % 11 18.33 % 0 0.00 %

S Yorks PAR-NEW-IN 1 162 0.59 % 4 2.47 % 150 92.59 % 10 6.17 % 8 4.94 % 0 0.00 %

England 162 20585 74.82 % 889 4.32 % 17501 85.02 % 1526 7.41 % 1335 6.49 % 860 4.18 %

Wales - High TB Carms PAR-HERD-S 5 455 1.65 % 38 8.35 % 401 88.13 % 24 5.27 % 15 3.30 % 1 0.22 %

PAR-PERSIS 17 2,952 10.73 % 133 4.51 % 2702 91.53 % 139 4.71 % 104 3.52 % 13 0.44 %

PAR-RAPID 11 63 0.23 % 25 39.68 % 34 53.97 % 8 12.70 % 4 6.35 % 0 0.00 %

SER-FLEXI 4 105 0.38 % 6 5.71 % 93 88.57 % 6 5.71 % 6 5.71 % 0 0.00 %

Ceredigion PAR-PERSIS 4 310 1.13 % 17 5.48 % 279 90.00 % 17 5.48 % 14 4.52 % 0 0.00 %

PAR-RAPID 4 5 0.02 % 4 80.00 % 1 20.00 % 1 20.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Gwent PAR-PERSIS 3 392 1.42 % 18 4.59 % 347 88.52 % 31 7.91 % 26 6.63 % 1 0.26 %

PAR-RAPID 8 36 0.13 % 10 27.78 % 22 61.11 % 4 11.11 % 3 8.33 % 1 2.78 %

Pembs PAR-HERD-S 3 49 0.18 % 3 6.12 % 45 91.84 % 1 2.04 % 1 2.04 % 0 0.00 %

PAR-OTHER 5 857 3.11 % 30 3.50 % 767 89.50 % 70 8.17 % 60 7.00 % 0 0.00 %

PAR-PERSIS 6 397 1.44 % 18 4.53 % 357 89.92 % 27 6.80 % 21 5.29 % 1 0.25 %

PAR-RAPID 13 33 0.12 % 7 21.21 % 25 75.76 % 2 6.06 % 1 3.03 % 0 0.00 %

SER-FLEXI 1 3 0.01 % 1 33.33 % 2 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %

Powys PAR-HERD-S 3 3 0.01 % 0 0.00 % 2 66.67 % 0 0.00 % 0 0.00 % 1 33.33 %




Table 2a. Summary by County for January 2019 Cont.




PAR-NEW-IN 2 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %

PAR-PERSIS 2 213 0.77 % 31 14.55 % 172 80.75 % 14 6.57 % 10 4.69 % 0 0.00 %

PAR-RAPID 4 34 0.12 % 4 11.76 % 11 32.35 % 2 5.88 % 2 5.88 % 17 50.00 %

SER-FLEXI 1 14 0.05 % 1 7.14 % 13 92.86 % 0 0.00 % 0 0.00 % 0 0.00 %

S Wales PAR-HERD-S 1 3 0.01 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

W Glamorgan PAR-HERD-S 2 117 0.43 % 7 5.98 % 108 92.31 % 5 4.27 % 2 1.71 % 0 0.00 %

PAR-PERSIS 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %

Wales - Intermediate TB Carms PAR-OTHER 1 20 0.07 % 2 10.00 % 12 60.00 % 6 30.00 % 6 30.00 % 0 0.00 %

Ceredigion PAR-HERD-S 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %

NE Wales PAR-NEW-IN 7 515 1.87 % 18 3.50 % 456 88.54 % 37 7.18 % 31 6.02 % 10 1.94 %

PAR-PERSIS 1 6 0.02 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-RAPID 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 8 248 0.90 % 19 7.66 % 166 66.94 % 10 4.03 % 10 4.03 % 53 21.37 %


Wales - Low TB Gwynedd PAR-NEW-IN 2 28 0.10 % 0 0.00 % 26 92.86 % 1 3.57 % 1 3.57 % 1 3.57 %


Wales 127 6928 25.18 % 394 5.69 % 6098 88.02 % 411 5.93 % 322 4.65 % 114 1.65 %

Grand Total Sum: 289 27513 1283 23599 1937 1657 974

*For test criteria please refer to Operational notes.




Table 2b. Summary by County for 2019




England - HRA Avon PAR-PERSIS 2 200 0.73 % 12 6.00 % 169 84.50 % 20 10.00 % 17 8.50 % 2 1.00 %

Corn & Scilly PAR-CUL-N 7 697 2.53 % 27 3.87 % 638 91.54 % 41 5.88 % 32 4.59 % 0 0.00 %

PAR-CUL-P 2 989 3.59 % 17 1.72 % 873 88.27 % 119 12.03 % 97 9.81 % 2 0.20 %

PAR-PERSIS 2 106 0.39 % 1 0.94 % 9 8.49 % 1 0.94 % 1 0.94 % 95 89.62 %

SER-FLEXI 2 15 0.05 % 0 0.00 % 15 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Devon PAR-CUL-N 8 810 2.94 % 65 8.02 % 694 85.68 % 59 7.28 % 50 6.17 % 1 0.12 %

PAR-CUL-P 2 441 1.60 % 18 4.08 % 401 90.93 % 29 6.58 % 22 4.99 % 0 0.00 %

PAR-NEW-IN 1 344 1.25 % 67 19.48 % 242 70.35 % 42 12.21 % 35 10.17 % 0 0.00 %

PAR-PERSIS 3 396 1.44 % 25 6.31 % 330 83.33 % 42 10.61 % 41 10.35 % 0 0.00 %

SER-FLEXI 2 62 0.23 % 6 9.68 % 25 40.32 % 0 0.00 % 0 0.00 % 31 50.00 %

Dorset PAR-CUL-N 5 378 1.37 % 23 6.08 % 314 83.07 % 41 10.85 % 40 10.58 % 1 0.26 %

PAR-CUL-P 2 869 3.16 % 38 4.37 % 795 91.48 % 37 4.26 % 36 4.14 % 0 0.00 %

Gloucs PAR-CUL-N 6 620 2.25 % 30 4.84 % 446 71.94 % 53 8.55 % 44 7.10 % 100 16.13 %

Heref PAR-CUL-N 2 343 1.25 % 15 4.37 % 311 90.67 % 18 5.25 % 17 4.96 % 0 0.00 %

Shropshire PAR-OTHER 2 454 1.65 % 24 5.29 % 392 86.34 % 44 9.69 % 38 8.37 % 0 0.00 %

PAR-PERSIS 1 72 0.26 % 2 2.78 % 56 77.78 % 15 20.83 % 14 19.44 % 0 0.00 %

Somerset PAR-CUL-P 1 228 0.83 % 21 9.21 % 196 85.96 % 17 7.46 % 9 3.95 % 2 0.88 %

PAR-OTHER 2 238 0.87 % 34 14.29 % 155 65.13 % 8 3.36 % 8 3.36 % 41 17.23 %

Wiltshire PAR-OTHER 1 36 0.13 % 1 2.78 % 33 91.67 % 4 11.11 % 2 5.56 % 0 0.00 %

SER-FLEXI 1 2 0.01 % 0 0.00 % 1 50.00 % 1 50.00 % 1 50.00 % 0 0.00 %

England - Edge Berks PAR-NEW-IN 7 1,009 3.67 % 23 2.28 % 921 91.28 % 72 7.14 % 65 6.44 % 0 0.00 %

PAR-PERSIS 1 92 0.33 % 4 4.35 % 87 94.57 % 2 2.17 % 1 1.09 % 0 0.00 %

Bucks PAR-NEW-IN 1 153 0.56 % 12 7.84 % 138 90.20 % 7 4.58 % 3 1.96 % 0 0.00 %

Cheshire PAR-NEW-IN 23 4,679 17.01 % 108 2.31 % 4172 89.16 % 294 6.28 % 249 5.32 % 150 3.21 %

PAR-PERSIS 1 13 0.05 % 1 7.69 % 12 92.31 % 0 0.00 % 0 0.00 % 0 0.00 %

Derbyshire PAR-NEW-IN 18 2,797 10.17 % 85 3.04 % 2365 84.55 % 154 5.51 % 134 4.79 % 213 7.62 %

E Sussex PAR-NEW-IN 5 807 2.93 % 46 5.70 % 719 89.10 % 30 3.72 % 29 3.59 % 13 1.61 %

Hampshire PAR-NEW-IN 6 55 0.20 % 1 1.82 % 51 92.73 % 3 5.45 % 3 5.45 % 0 0.00 %




Table 2b. Summary by County for 2019 Cont.




SER-NSR 1 7 0.03 % 0 0.00 % 7 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Leics & Rut PAR-NEW-IN 5 475 1.73 % 22 4.63 % 316 66.53 % 54 11.37 % 50 10.53 % 87 18.32 %

Northants PAR-NEW-IN 1 6 0.02 % 0 0.00 % 5 83.33 % 2 33.33 % 1 16.67 % 0 0.00 %

Notts PAR-NEW-IN 3 377 1.37 % 15 3.98 % 259 68.70 % 44 11.67 % 40 10.61 % 63 16.71 %

Oxon PAR-NEW-IN 17 1,858 6.75 % 101 5.44 % 1570 84.50 % 198 10.66 % 187 10.06 % 0 0.00 %

Warks PAR-NEW-IN 10 546 1.98 % 24 4.40 % 428 78.39 % 38 6.96 % 35 6.41 % 59 10.81 %

PAR-PERSIS 2 157 0.57 % 9 5.73 % 137 87.26 % 12 7.64 % 11 7.01 % 0 0.00 %

England - LRA Cambs PAR-NEW-IN 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %

Cumbria PAR-NEW-IN 1 3 0.01 % 0 0.00 % 2 66.67 % 1 33.33 % 1 33.33 % 0 0.00 %

Lancashire PAR-NEW-IN 1 22 0.08 % 0 0.00 % 21 95.45 % 1 4.55 % 1 4.55 % 0 0.00 %

Lincs PAR-NEW-IN 3 60 0.22 % 8 13.33 % 41 68.33 % 11 18.33 % 11 18.33 % 0 0.00 %

S Yorks PAR-NEW-IN 1 162 0.59 % 4 2.47 % 150 92.59 % 10 6.17 % 8 4.94 % 0 0.00 %

England 162 20585 74.82 % 889 4.32 % 17501 85.02 % 1526 7.41 % 1335 6.49 % 860 4.18 %

Wales - High TB Carms PAR-HERD-S 5 455 1.65 % 38 8.35 % 401 88.13 % 24 5.27 % 15 3.30 % 1 0.22 %

PAR-PERSIS 17 2,952 10.73 % 133 4.51 % 2702 91.53 % 139 4.71 % 104 3.52 % 13 0.44 %

PAR-RAPID 11 63 0.23 % 25 39.68 % 34 53.97 % 8 12.70 % 4 6.35 % 0 0.00 %

SER-FLEXI 4 105 0.38 % 6 5.71 % 93 88.57 % 6 5.71 % 6 5.71 % 0 0.00 %

Ceredigion PAR-PERSIS 4 310 1.13 % 17 5.48 % 279 90.00 % 17 5.48 % 14 4.52 % 0 0.00 %

PAR-RAPID 4 5 0.02 % 4 80.00 % 1 20.00 % 1 20.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

Gwent PAR-PERSIS 3 392 1.42 % 18 4.59 % 347 88.52 % 31 7.91 % 26 6.63 % 1 0.26 %

PAR-RAPID 8 36 0.13 % 10 27.78 % 22 61.11 % 4 11.11 % 3 8.33 % 1 2.78 %

Pembs PAR-HERD-S 3 49 0.18 % 3 6.12 % 45 91.84 % 1 2.04 % 1 2.04 % 0 0.00 %

PAR-OTHER 5 857 3.11 % 30 3.50 % 767 89.50 % 70 8.17 % 60 7.00 % 0 0.00 %

PAR-PERSIS 6 397 1.44 % 18 4.53 % 357 89.92 % 27 6.80 % 21 5.29 % 1 0.25 %

PAR-RAPID 13 33 0.12 % 7 21.21 % 25 75.76 % 2 6.06 % 1 3.03 % 0 0.00 %

SER-FLEXI 1 3 0.01 % 1 33.33 % 2 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %

Powys PAR-HERD-S 3 3 0.01 % 0 0.00 % 2 66.67 % 0 0.00 % 0 0.00 % 1 33.33 %




Table 2b. Summary by County for 2019 Cont.




PAR-NEW-IN 2 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %

PAR-PERSIS 2 213 0.77 % 31 14.55 % 172 80.75 % 14 6.57 % 10 4.69 % 0 0.00 %

PAR-RAPID 4 34 0.12 % 4 11.76 % 11 32.35 % 2 5.88 % 2 5.88 % 17 50.00 %

SER-FLEXI 1 14 0.05 % 1 7.14 % 13 92.86 % 0 0.00 % 0 0.00 % 0 0.00 %

S Wales PAR-HERD-S 1 3 0.01 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %


PAR-PERSIS 1 7 0.03 % 0 0.00 % 5 71.43 % 2 28.57 % 2 28.57 % 0 0.00 %

Wales - Intermediate TB Carms PAR-OTHER 1 20 0.07 % 2 10.00 % 12 60.00 % 6 30.00 % 6 30.00 % 0 0.00 %

Ceredigion PAR-HERD-S 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %


PAR-PERSIS 1 6 0.02 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

PAR-RAPID 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %

SER-FLEXI 8 248 0.90 % 19 7.66 % 166 66.94 % 10 4.03 % 10 4.03 % 53 21.37 %


Wales - Low TB Gwynedd PAR-NEW-IN 2 28 0.10 % 0 0.00 % 26 92.86 % 1 3.57 % 1 3.57 % 1 3.57 %


Wales 127 6928 25.18 % 394 5.69 % 6098 88.02 % 411 5.93 % 322 4.65 % 114 1.65 %

Grand Total Sum: 289 27513 1283 23599 1937 1657 974

*For test criteria please refer to Operational notes.




Table 3a. Summary of IFN Gamma results by country and protocol January 2019

Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject

n % of total n % n % n % n % n %

England Parallel 156 20499 99.6% 883 4.3% 17453 85.1% 1525 7.4% 1334 6.5% 829 4.0%

Serial/Extended 6 86 0.4% 6 7.0% 48 55.8% 1 1.2% 1 1.2% 31 36.0%

Total 162 20585 100.0% 889 4.3% 17501 85.0% 1526 7.4% 1335 6.5% 860 4.2%

Scotland Parallel 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Total 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Wales Parallel 112 6557 94.6% 367 5.6% 5823 88.8% 395 6.0% 306 4.7% 61 0.9%

Serial/Extended 15 371 5.4% 27 7.3% 275 74.1% 16 4.3% 16 4.3% 53 14.3%

Total 127 6928 100.0% 394 5.7% 6098 88.0% 411 5.9% 322 4.6% 114 1.6%

GB Parallel 268 27056 98.3% 1250 4.6% 23276 86.0% 1920 7.1% 1640 6.1% 890 3.3%

Serial/Extended 21 457 1.7% 33 7.2% 323 70.7% 17 3.7% 17 3.7% 84 18.4%

Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0% 1657 6.0% 974 3.5%

162.00

127.00

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.

Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)

Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject


England Parallel 156 20499 99.6% 883 4.3% 17453 85.1% 1525 7.4% 1334 6.5% 829 4.0%

Serial/Extended 6 86 0.4% 6 7.0% 48 55.8% 1 1.2% 1 1.2% 31 36.0%

Total 162 20585 100.0% 889 4.3% 17501 85.0% 1526 7.4% 1335 6.5% 860 4.2%

Scotland Parallel 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Total 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %

Wales Parallel 112 6557 94.6% 367 5.6% 5823 88.8% 395 6.0% 306 4.7% 61 0.9%

Serial/Extended 15 371 5.4% 27 7.3% 275 74.1% 16 4.3% 16 4.3% 53 14.3%

Total 127 6928 100.0% 394 5.7% 6098 88.0% 411 5.9% 322 4.6% 114 1.6%

GB Parallel 268 27056 98.3% 1250 4.6% 23276 86.0% 1920 7.1% 1640 6.1% 890 3.3%

Serial/Extended 21 457 1.7% 33 7.2% 323 70.7% 17 3.7% 17 3.7% 84 18.4%

Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0% 1657 6.0% 974 3.5%

162.00

127.00

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.

Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject


GB

Extended Flexible IFN-GAMMA test 20 450 1.6% 33 7.3% 316 70.2% 17 3.8 % 17 3.8 % 84 18.7 %

Parallel - Herd or Group Slaughter 16 634 2.3% 50 7.9% 563 88.8% 31 4.9 % 19 3.0 % 2 0.3 %

Parallel New Infection 119 13956 50.7% 534 3.8% 11923 85.4% 1004 7.2 % 888 6.4 % 611 4.4 %

Parallel - new infection cull area 28 2848 10.4% 160 5.6% 2403 84.4% 212 7.4 % 183 6.4 % 102 3.6 %

Parallel - Other 11 1605 5.8% 91 5.7% 1359 84.7% 132 8.2 % 114 7.1 % 41 2.6 %

Parallel Persistent Infection 46 5313 19.3% 271 5.1% 4668 87.9% 322 6.1 % 262 4.9 % 112 2.1 %

Parallel - persistent infection cull area 7 2527 9.2% 94 3.7% 2265 89.6% 202 8.0 % 164 6.5 % 4 0.2 %

Parallel - Rapid Testing Two-times IRs 41 173 0.6% 50 28.9% 95 54.9% 17 9.8 % 10 5.8 % 18 10.4 %

Serial - Suspected NSR Herd 1 7 0.0% 0 0.0 % 7 100.0% 0 0 0

Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0 % 1657 6.0 % 974 3.5 %

Table 4a. Summaryof IFN Gamma results by submission reason (January 2019)

England - HRA Extended Flexible IFN-GAMMA test 5 79 0.3% 6 7.6% 41 51.9% 1 1.3 % 1 1.3 % 31 39.2 %

Parallel New Infection 1 344 1.3% 67 19.5% 242 70.3% 42 12.2 % 35 10.2 % 0


Parallel - Other 5 728 2.6% 59 8.1% 580 79.7% 56 7.7 % 48 6.6 % 41 5.6 %



England - HRA 54 7300 26.5% 426 5.8% 6095 83.5% 591 8.1 % 504 6.9 % 275 3.8 %

England - Edge Parallel New Infection 96 12762 46.4% 437 3.4% 10944 85.8% 896 7.0 % 796 6.2 % 585 4.6 %

Parallel Persistent Infection 4 262 1.0% 14 5.3% 236 90.1% 14 5.3 % 12 4.6 % 0


England - Edge 101 13031 47.4% 451 3.5% 11187 85.8% 910 7.0 % 808 6.2 % 585 4.5 %

England - LRA Parallel New Infection 7 254 0.9% 12 4.7% 219 86.2% 25 9.8 % 23 9.1 % 0

England - LRA 7 254 0.9% 12 4.7% 219 86.2% 25 9.8 % 23 9.1 % 0

Wales - High TB Extended Flexible IFN-GAMMA test 7 123 0.4% 8 6.5% 109 88.6% 6 4.9 % 6 4.9 % 0


Parallel New Infection 2 7 0.0% 0 0.0 % 5 71.4% 2 28.6 % 2 28.6 % 0

Parallel - Other 5 857 3.1% 30 3.5% 767 89.5% 70 8.2 % 60 7.0 % 0



Wales - High TB 101 6056 22.0% 353 5.8% 5395 89.1% 355 5.9 % 273 4.5 % 35 0.6 %

Wales - Intermediate TB Extended Flexible IFN-GAMMA test 8 248 0.9% 19 7.7% 166 66.9% 10 4.0 % 10 4.0 % 53 21.4 %

Parallel - Herd or Group Slaughter 2 7 0.0% 2 28.6% 4 57.1% 1 14.3 % 1 14.3 % 0


Parallel - Other 1 20 0.1% 2 10.0% 12 60.0% 6 30.0 % 6 30.0 % 0

Parallel Persistent Infection 1 6 0.0% 0 0.0 % 6 100.0% 0 0 0

Parallel - Rapid Testing Two-times IRs 1 2 0.0% 0 0.0 % 2 100.0% 0 0 0

Wales - Intermediate TB 20 798 2.9% 41 5.1% 646 81.0% 54 6.8 % 48 6.0 % 63 7.9 %

Wales - Low TB Parallel New Infection 6 74 0.3% 0 0.0 % 57 77.0% 2 2.7 % 1 1.4 % 16 21.6 %

Wales - Low TB 6 74 0.3% 0 0.0 % 57 77.0% 2 2.7 % 1 1.4 % 16 21.6 %

Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject


GB

Extended Flexible IFN-GAMMA test 20 450 1.6% 33 7.3% 316 70.2% 17 3.8 % 17 3.8 % 84 18.7 %




Parallel - Other 11 1605 5.8% 91 5.7% 1359 84.7% 132 8.2 % 114 7.1 % 41 2.6 %





Total 289 27513 100.0% 1283 4.7% 23599 85.8% 1937 7.0 % 1657 6.0 % 974 3.5 %

Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)

England - HRA Extended Flexible IFN-GAMMA test 5 79 0.3% 6 7.6% 41 51.9% 1 1.3 % 1 1.3 % 31 39.2 %

Parallel New Infection 1 344 1.3% 67 19.5% 242 70.3% 42 12.2 % 35 10.2 % 0


Parallel - Other 5 728 2.6% 59 8.1% 580 79.7% 56 7.7 % 48 6.6 % 41 5.6 %



England - Edge Parallel New Infection 96 12762 46.4% 437 3.4% 10944 85.8% 896 7.0 % 796 6.2 % 585 4.6 %

Parallel Persistent Infection 4 262 1.0% 14 5.3% 236 90.1% 14 5.3 % 12 4.6 % 0


England - LRA Parallel New Infection 7 254 0.9% 12 4.7% 219 86.2% 25 9.8 % 23 9.1 % 0

England 162 20585 74.8% 889 4.3% 17501 85.0% 1526 7.4 % 1335 6.5 % 860 4.2 %

Wales - High TB Extended Flexible IFN-GAMMA test 7 123 0.4% 8 6.5% 109 88.6% 6 4.9 % 6 4.9 % 0


Parallel New Infection 2 7 0.0% 0 0.0 % 5 71.4% 2 28.6 % 2 28.6 % 0

Parallel - Other 5 857 3.1% 30 3.5% 767 89.5% 70 8.2 % 60 7.0 % 0


Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.


Wales - Intermediate TB Extended Flexible IFN-GAMMA test 8 248 0.9% 19 7.7% 166 66.9% 10 4.0 % 10 4.0 % 53 21.4 %

Parallel - Herd or Group Slaughter 2 7 0.0% 2 28.6% 4 57.1% 1 14.3 % 1 14.3 % 0


Parallel - Other 1 20 0.1% 2 10.0% 12 60.0% 6 30.0 % 6 30.0 % 0

Parallel Persistent Infection 1 6 0.0% 0 0.0 % 6 100.0% 0 0 0

Parallel - Rapid Testing Two-times IRs 1 2 0.0% 0 0.0 % 2 100.0% 0 0 0

Wales - Low TB Parallel New Infection 6 74 0.3% 0 0.0 % 57 77.0% 2 2.7 % 1 1.4 % 16 21.6 %

Wales 127 6928 25.2% 394 5.7% 6098 88.0% 411 5.9 % 322 4.6 % 114 1.6 %

Table 5a. Summary of PM and culture results for IFNg positive animals.

Year Month Herdssampled

Samplestested

Herdswithpositives

IFNg+samples

WrongEartags

No PMNoCult

No PMCultPend

No PMCultNeg

No PMCultMb

NoPMCultOther

VL NoCult

VLCultNeg

VLCultPend

VLCultMb

VLCultOther

NVLNoCult

NVLCultPend

NVLCultNeg

NVLCultMb

NVLCultOther

2018 February 206 20791 130 1229 3 22 0 0 0 0 72 7 1 8 0 1073 1 35 0 5

March 189 17292 131 1199 1 3 0 0 0 0 121 6 0 4 0 984 0 72 1 2

April 195 20517 118 953 4 16 0 0 0 0 116 1 0 0 0 748 0 66 1 1

May 250 21066 131 947 2 2 0 0 0 0 121 1 0 4 0 780 0 35 1 1

June 187 18891 110 701 2 2 0 0 0 0 78 2 0 12 0 579 0 25 0 0

July 207 24111 119 806 1 12 0 0 0 0 67 4 0 2 0 675 3 38 0 2

August 228 19599 120 682 4 3 0 0 0 0 64 2 0 8 1 569 0 31 0 0

September 216 21441 137 946 6 6 0 0 0 0 33 1 0 2 0 840 0 51 1 4

October 274 23976 152 1417 12 8 0 0 0 0 99 1 0 3 1 1231 5 40 0 1

November 282 24570 192 1580 2 5 0 0 0 0 42 0 4 7 4 1329 84 87 0 7

December 228 21554 147 1241 6 21 0 0 0 0 91 0 8 2 0 1025 87 0 0 0

2019 January 289 27513 179 1283 474 412 0 0 0 0 26 0 3 0 0 237 14 0 0 0

Totals (last12 months) 2751 261321 1666 12984 517 512 0 0 0 0 930 25 16 52 6 10070 194 480 4 23

Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

Country Herdssampled

Samplestested

Herdswithpositives

IFNg+samples

No PMNoCult

No PMCultPend

No PMCultNeg

No PMCultMb

NoPMCultOther

VL NoCult

VLCultNeg

VLCultPend

VLCultMb

VLCultOther

NVLNoCult

NVLCultPend

NVLCultNeg

NVLCultMb

NVLCultOther

England 1530 200003 1049 9852 271 0 0 0 0 775 15 7 34 4 8193 25 51 1 3

Scotland 26 1828 17 146 30 0 0 0 0 22 4 0 0 0 49 0 36 0 3

Wales 1195 59490 600 2986 211 0 0 0 0 133 6 9 18 2 1828 169 393 3 17

Totals 2751 261321 1666 12984 512 0 0 0 0 930 25 16 52 6 10070 194 480 4 23

Figure 4a. Number of herds sampled for IFNg and numbers of herds with anypositive IFNg results.

58

85

36

86

46

93

38

83

74

103

54

72

81

81

53

97

56

94

52

96

49

108

33

81

67

140

76

130

58

131

77

118

119

131

77

110

88

119

108

120

79

137

122

152

90

192

81

147

110

179

0

50

100

150

200

250

300

JanuaryFebruaryM

archAprilM

ayJuneJulyAugustSeptem

berO

ctoberN

ovember

Decem

berJanuaryFebruaryM

archAprilM

ayJuneJulyAugustSeptem

berO

ctoberN

ovember

Decem

berJanuary

2017

2018

2019

IFNg Positive herds IFNg Negative herds

9059

420

9793

442

10459

573

10179

906

13951

615

11775

419

11040

437

10097

560

10063

664

13635

971

9492

961

8778

66818401

969

19186

1229

15561

1199

18776

953

19795

947

17980

701

22955

806

18566

682

20202

946

22357

1416

22496

1580

20038

1241

25652

1283

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25000

30000

January

February

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April

May

June

July

August

September

October

Novem

ber

Decem

ber

January

2017

2018

2019

IFNg positive animals IFNg non positive animals*

Figure 4b. Number of animals sampled for IFNg and numbers of animals withpositive IFNg results.

*Includes all animals with IFNg test negative, resample and reject outcomes

0

1

2

3

4

5

6

7

8

Janu

ary

Febr

uary

Mar

ch

April

May

June July

Augu

st

Sept

embe

r

Oct

ober

Nov

embe

r

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embe

r

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embe

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2018

2019

%Rejected %Resampled % NEG Fail % POS Fail

% of totalsubmitted

Figure 5. Rates of resamples including the fail rates broken down for positive(POS) and negative (NEG) controls.

0 10 20 30 40 50 60 70

Wiltshire

Warks

S Wales

Shropshire

Pembs

Notts

NE Wales

Leics & Rut

Heref

Gwynedd

Gloucs

Dorset

Derbyshire

Corn & Scilly

Ceredigion

Cambs

Berks

%Retested %Resampled

Figure 6. Proportion of samples retested, or requiring a resample, during January 2019 (% submission perCounty) County %Resampled %Retested

Avon 8.50 % 10.00 %

Berks 5.99 % 6.72 %

Bucks 1.96 % 4.58 %

Cambs 28.57 % 28.57 %

Carms 3.76 % 5.09 %

Ceredigion 4.73 % 5.99 %

Cheshire 5.31 % 6.27 %

Corn & Scilly 7.19 % 8.91 %

Cumbria 33.33 % 33.33 %

Derbyshire 4.79 % 5.51 %

Devon 7.21 % 8.38 %

Dorset 6.09 % 6.26 %

E Sussex 3.59 % 3.72 %

Gloucs 7.10 % 8.55 %

Gwent 6.78 % 8.18 %

Gwynedd 3.57 % 3.57 %

Hampshire 4.84 % 4.84 %

Heref 4.96 % 5.25 %

Lancashire 4.55 % 4.55 %

Leics & Rut 10.53 % 11.37 %

Lincs 18.33 % 18.33 %

NE Wales 5.02 % 5.88 %

Northants 16.67 % 33.33 %

Notts 10.61 % 11.67 %

Oxon 10.06 % 10.66 %

Pembs 6.20 % 7.47 %

Powys 5.17 % 6.64 %

Shropshire 9.89 % 11.22 %

Somerset 3.65 % 5.36 %

S Wales

S Yorks 4.94 % 6.17 %

Warks 6.54 % 7.11 %

W Glamorgan 3.08 % 5.38 %

Wiltshire 7.89 % 13.16 %

Country No.animals

No of IFHg+animals

% Positiveanimals No. samples No of IFHg+

samples% Positivesamples

England 20087 889 4.4% 20585 889 4.3%

Wales 6848 394 5.8% 6928 394 5.7%

GB 26935 1283 4.8% 27513 1283 4.7%

Table 6a (i): Number of animals and submissions tested in January 2019

Country No.animals

No of IFHg+animals



England 20087 889 4.4% 20585 889 4.3%

Wales 6848 394 5.8% 6928 394 5.7%

GB 26935 1283 4.8% 27513 1283 4.7%

Table 6b (i): Number of animals and submissions tested in 2019 Up to end of January

Country No.animals

No of IFHg+animals



England 14147 657 4.6% 14220 657 4.6%

Wales 4813 277 5.8% 5045 277 5.5%

Scotland 410 35 8.5% 413 35 8.5%

GB 19370 969 5.0% 19678 969 4.9%

Table 6a (ii): Number of animals and submissions tested in January 2018

Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of January

Country No.animals

No of IFHg+animals



England 14147 657 4.6% 14220 657 4.6%

Wales 4813 277 5.8% 5045 277 5.5%

Scotland 410 35 8.5% 413 35 8.5%

GB 19370 969 5.0% 19678 969 4.9%

* Test type NumSubmissions Num Samples Num Positives

Private % Positive

Gloucestershire PRV-SP 1 4 0

Somerset excl North PRV-SE 1 4 1 25.00 %

Wiltshire PRV-SP 1 3 0

Total 3 11 1 9.09 %

*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;PRV-SP: Private High Specificity test – England

Table 7a Number of private samples January 2019

Table 7b Number of private samples 2019

* Test type NumSubmissions

NumSamples

NumPositivesPrivate

% Positive

Gloucestershire PRV-SP 1 4 0

Somerset excl North PRV-SE 1 4 1 25.00 %

Wiltshire PRV-SP 1 3 0

Total 3 11 1 9.09 %

Isle of Man SER-IOM 3 19 0

Isle of Man SER-IOM 3 19 0

Test County Num Submissions Num Samples Tested Num Positive Samples % Positive

TC0077 North East Wales 2 97 5 5.2 %TC0077 Sum: 97 5 5.2 %

Table 8a Number of Cattle Antibody Tests January 2019

Test County Num Submissions Num Samples Tested Num Positive Samples % Positive

TC0077 North East Wales 2 97 5 5.2 %TC0077 Sum: 97 5 5.2 %

Table 8b Number of Cattle Antibody Tests 2019

monthly report: surveillance project sb4008 ifng tests for

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