monitoring of phytophthora spp on potato crops …...monitoring of phytophthora spp on potato crops...

Monitoring of Phytophthora spp on potato crops and Solanaceous host

in Latin America

17th EuroBlight 2019 Workshop, York, UK, 12-15 May 2019

M. Florencia Lucca1, Silvia Restrepo2, Giovanna Danies3, Ivette Acuña4, Samantha Zanotta5

1Potato Research Group, National Agricultural Technology Institute (INTA), Balcarce Argentina, 2Department of Biological Sciences, Universidad de Los Andes, Bogotá, Colombia, 3Department of Design, Universidad de los Andes, Bogotá, Colombia, 4Institute of Agricultural Research, Chile, 5Biological Institute, Sao Pablo, Brazil

Introduction

Late blight

• Most important disease in potato and tomato

• Large outbreaks on semi-domesticated plants such as tree tomato

(Solanum betaceum) and other Solanaceous plants

TizonLatino – Fontagro Project

• Genotyping based on a multiplex of 12 SSR markers (EuroBlight) to study

populations of P. infestans in Latin America Genetic changes in

populations

New Phytophthora species

• P. betacei, was described infecting tree tomato crops in Colombia


• P. infestans in potato in the central region of Colombia

• New species: P. betacei in tree tomato in Colombia

• P. infestans in tomato in Colombia

• P. infestans in Chile, Argentina and Brazil in potato

• Future actions of TizonLatino and Fontagro Project


Phytophthora spp. in Latin America


P. infestans in potato in the central region of Colombia

• 279 Phytophthora infestans isolates

• 11 different potato cultivars

• 11 different geographical localities


Phytophthora infestans in the central region of Colombia

Phureja Group

• Criolla Colombia (n=47)

• Criolla Dorada (n=22)

• Criolla Ocarina (n=9)

• Milagros (n=1)

• Paola (n=8)

Andigenum Group

• Diacol Capiro (n=47)

• Parda Pastusa (n=12)

• Superior (n=62)

• Pastusa Suprema (n=53)

• Tuquerrena(n=3)

• ICA Unica(n=15)


Host cultivars

North (N): Tausa (n=24), Cogua (n=3), and Zipaquira (n=18)

West (W): Subachoque (n=53), El Rosal (n=2), Bojaca (n=17),

and Mosquera (n=2)

Central (C): Bogota (n=53)

North East (NE): Villapinzon (n=55), Choconta (n=7), and

Sesquile (n=45)

Geographical localities


RG57 : EC-1

Frequency of MLGs in the 11 localities sampled


Genetic diversity of P. infestans isolates in central Colombia

Frequency of MLGs in the 11 potato cultivars

SSR Multiplex: 76 MLGs


Population genetic structure of the P. infestans isolates according to geographical origin


Population genetic structure of the P. infestans isolates according to potato cultivars

• Neither geographic location nor the potato genotype significantly shaped

the population structure of the P. infestans population.

• The populations are composed by a high number of genotypes but with a

high similarity among them.

• The absence of genetic structure according to the host cultivar suggests a

high level of migration or gene flow among different host cultivars.

• No evidence of sexual reproduction was detected for P. infestans isolates

collected from potato crops (A1 mating type).

• For the first time, the subclonal variation was thoroughly described in the

most important potato production region in Colombia


Conclusions of Phytophthora infestans in the central region of Colombia


P. betacei in tree tomato in Colombia


P. betacei in tree tomato in Colombia (Nariño State)

Pan_Nar_EC

P. infestans - S. tuberosum

Southern region

P. andina - Anarrichoenum complex - EC

P. andina - Naranjilla - EC

P. infestans - S. lycopersicum - US

P. infestans - S. tuberosum

Central region

P. betacei - S. betaceum

Southern region

P. betacei - S. betaceum

Central region

DA eigenvalues


P. betacei in tree tomato in the central region of Colombia (Cundinamarca State)

• A new Phytophthora species, P. betacei, was described infecting tree

tomato crops in Southern Colombia (Nariño State).

• The latest results also permitted to reinforce and support the description

of the new species and describe the expanded geographical range of P.

betacei in the central region of Colombia (Cundinamarca State).

Conclusions of P. betacei in tree tomato in Colombia


• Phenotypic characterization:

Mating type assay

Sensitivity to fungicides: mefenoxam, cymoxanil and fluopicolide.

Pathogenicity on tomato and potato

• Genotypic characterization:

Mitochondrial haplotype

Microsatellite marker analysis (SSRs).

Phenotypic and genotypic characterization of isolatesof Phytophthora infestans on commercial tomato(Solanum lycopersicum) crops in the Andean Region ofColombiaAndrea Olave1, 2 , Silvia Restrepo1, Florencia Lucca3, William E. Fry4, Kevin L. Myers4, Giovanna Danies5,*, and Mauricio Soto-Suárez2,


https://tizonlatino.github.io/


DNA

Panel of 12 microsatellite markers (Euroblight)


Genotyping of Phytophthora spp. populations in Latin America

CONICET INDEAR

INTA

FIL

Genomic Unit (Biotechnology Institute) & Balcarce Research Station

Argentine Consortium of Genomic Technology: CATG



ArgentinaTucumán (Pedemonte and Tafí del Valle)Córdoba (Córdoba and Villa Dolores)Southeast of Buenos Aires ProvinceAR_1 to AR 5 (‘90)

ChileCoquimboLos RíosLa AraucaníaLos LagosValparaísoUS-1 (until 2006)

BrasilParanáRio Grande so SulSao PabloMinas GeraisBR-1

EU 2_A1

EU 2_A1

EU 2_A1 US-1 (in Tomato)

Genotyping at Genomic Unit - INTA

POPULATION

Pin_Stub_ARPin_Stub_BRPin_Stub_CH

Samples/Node

1

25

33

0.012 0.054 0.096 0.138 0.18

DISTANCE

MLG.48

MLG.47

MLG.33

MLG.46MLG.39

MLG.42

MLG.36

MLG.37

MLG.43

MLG.40

MLG.44

MLG.45MLG.38

MLG.35

MLG.34

MLG.41

MLG.4

MLG.28

MLG.21

MLG.31

MLG.29MLG.11

MLG.27

MLG.18

MLG.17

MLG.19

MLG.24

MLG.26

MLG.15

MLG.20

MLG.25

MLG.23

MLG.22

MLG.16

MLG.10MLG.32

MLG.5

MLG.30

MLG.3

MLG.12

MLG.14

MLG.7MLG.2

MLG.8

MLG.1

MLG.13

MLG.9

MLG.6


Argentina, Brazil and Chile (2017-18)

Alerta temprana para el manejo del Tizón tardío de la papa

ATN/RF 16678-RG


Future actions of TizonLatino - Fontagro

• FontagroWorkpackage 4: Monitoring of casual agentTraining and outreach activitiesGenotyping / phenotyping (Ecuador, Chile, Argentina and Panamá)Map of P. infestans populations

• TizonLatinoGenotyping 50 samples from different countries of Latin America at INTA Argentina

(FTA cards was sent to each country)Next meeting of TizonLatino network in 2020 in León, México (ALAP 2020 Meeting)

• Global Blight Network Database to analyze the dynamic of P. infestans populations

Training and outreach activities


Alerta temprana para el manejo del Tizón tardío de la papa

ATN/RF 16678-RG


Future actions of TizonLatino - Fontagro

• FontagroWorkpackage 4: Monitoring of casual agentTraining and outreach activitiesGenotyping / phenotyping (Ecuador, Chile, Argentina and Panamá)Map of P. infestans populations

• TizonLatinoGenotyping 50 samples from different countries of Latin America at INTA Argentina

(FTA cards was sent to each country)Next meeting of TizonLatino network in 2020 in León, México (ALAP 2020 Meeting)

• Global Blight Network Database to analyze the dynamic of P. infestans populations

monitoring of phytophthora spp on potato crops …...monitoring of phytophthora spp on potato crops...

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