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Molecular BiologyCatalogue 2016

Outline

p. 1-2

p. 3-9p. 3p. 7

p. 10-11p. 11p. 11

p. 12-21

p. 13

p. 14p. 13

p. 14p. 15p. 15p. 16p. 16p. 17p. 18p. 19p. 19

p. 22-35

p. 22

p. 32p. 28

p. 34

p. 36-38

p. 36p. 37p. 38

2. PCR Applications

1. Nucleic Acids Preparation

DNA PolymerasesOther PCR Reagents (Ladder, dNTPs, Enhancers)

3. RT-PCR ApplicationsRT-PCR ReagentscDNA Synthesis Kits

4. qPCR Applications

Be BrilliantSybrGreen ReagentsProbes-based ReagentsHRM ReagentsqPCR-Multiplex ReagentsmiRNA SolutionsAlien qPCR Inhibitor AlertRT-qPCR ReagentsSideStep TechnologyqPCR plastics and consumablesMycoplasma Detection KitsReference RNA and DNA

5. Molecular Biology

Competent CellsCloningTransfectionMutagenesis

6. Gene Editing and Synthetic Biology

High-Throughput Mutagenesis QuikChange HTCRISPR-Cas9 System SureVector System

p. 39-47

p. 39p. 40p. 41p. 42p. 43p. 44p. 44p. 45p. 45p. 47

p. 48-49

p. 52p. 54p. 55

p. 50-51

p. 52-55

p. 56

7. Expression Systems

Viral vectorsBacterial vectorsUnicellular Eukaryotic Vectors (Yeast)Mammalian vectorsControl SystemsDual ExpressionFluorescenceLuminescenceIntracellular Signaling PathwaysProtein / Antibody Interactions

8. Proteins Applications - MARS Columns

9. Microarrays and NGS

10. Instruments

Samples AnalysisSamples Quality ControlNew TapeStation 4200 System

11. Contact us

NEW Sample Program ONLINE!www.agilent.com/genomics/SampleRequest

Nucleic Acid Preparation

DNA Extraction KitsDNA Extraction Kit - Ref #200600→ High Yields and FAST For all sample types : entire tissue, blood, cell culture…→ Non-toxic: NO phenol, NO chloroform→ Applications Southern Blott, PCR assays Cloning, Mutation

SourceBloodTissueCells

Quantity5-10mL

250mg -1g~10^8Cells

RecoverEase gDNA Isolation Kit

→ gDNA analysis from many tissues: liver, kidney, heart, bone marrow…→ Non-toxic→ gDNA from 100 to 500kb

720203720202

RecoverEase gDNA Isolation Kit, 15prepKit, RecoverEase gDNA Isolation, 30prep

Other DNA Kits

2030035500-0051

200391200392

DNA Ligation Kit, 150prepAgilent DNA Isolation Kit, 50prepKit Lambda DNA Purification, 25prepKit Lambda DNA Purification, 50prep

StrataPreps→ Purification of PCR products, gel and plasmids→ Pure DNA isolation with performance > 85%→ FAST→ DNA from 100pb to 23kb

400761400763400766400768400771400773400775900775

StrataPrep Plasmid Miniprep Kit, 50prepStrataPrep Plasmid Miniprep Kit, 250prepStrataPrep DNA Gel Extraction, 50prepStrataPrep DNA Gel Extraction, 250prepStrataPrep PCR Purification Kit, 50prepStrataPrep PCR Purification Kit, 250prepStrataPrep 96 PCR Purification, 2 platesStrataPrep 96 PCR Purification, 50 plates

RecoverEase gNA Isolation Kit, 15prepKit, RecoverEase gDNA Isolation, 30prep

1For Research Use Only. Not for use in diagnostic procedures.

DNA’s Yield> 30-100 μg

> 250-1100 μg> 600-1000 μg

RNA Extraction KitsAbsolutely RNA extraction Kits→ FAST ≈ 30 min→ Simple method for purifying RNA from tissues and cells→ Ready to use in various formats Columns, magnetic beads Minipreps, micropreps, nanopreps FFPE preps and High-Through put 96 plates→ Applications (q)RT-PCR, miRNA detection and analysis Northern, in vitro transcribed RNA Micro-dissection laser, microarray target generation,…

400753400754400792400793400800400805400806400809400811400814200344200345

5185-60005188-2780

Absolutely RNA Nanoprep Kit, 50prepAbsolutely RNA Nanoprep Spin Cups, x12Absolutely RNA Lysis Buffer, 125 mLAbsolutely RNA 96 Microprep Kit, 2 platesAbsolutely RNA Miniprep Kit, 50prepAbsolutely RNA Microprep Kit, 50prepAbsolutely mRNA purification kit, 10prepAbsolutely RNA FFPE Kit, 50prepAbsolutely RNA FFPE Kt(without deparaffinization reagents), 50prepAbsolutely miRNA Kit, 50prepmiRNA Extraction KitRNA Isolation KitMini kit of total RNA isolation, 50prepMini kit Plants RNA isolation, 50prep

Absolutely RNA® Miniprep

Absolutely RNA®

Microprep

Absolutely RNA® 96

Microprep

Absolutely RNA®

Nanoprep

Absolutely RNA® FFPE

Absolutely RNA miRNA

Absolutely mRNA

Sample Size

105 to 107 cells5 -40 mg tissue

Up to 5 x 105

cellsUp to 5 x 105

cells

1 x 104 cells down to a single cell

FFPE tis-sue

1-5x106 cells1-25 mg tissue

Up to 1 mg of total RNA

Format Spin cup +pre-filter Spin cup Spin cup Spin cup Spin cup Spin cup +

pre-filter

Customized oligo-dT magnetic particles

Capacity > 200 μg RNA 50 μg RNA 50 μg RNAApprox. 200 ng RNA

50 μg ~50 μg

1 mg of total RNA/ 1mg of magnetic

particlesElution Volume 50 μl 30 μl 30 μl 10 μl 30 μl 50 μl 10-50 μl



PCR Applications

Polymerase Decision Tree



High Fidelity PolymerasesPfu Ultra II Fusion DNA Polymerase

→ FIDELITY higher of the market→ 15 sec/kb from target up to 23kb→ 1 error per 2.5 million/bp→ Available MasterMix and HotStart format

600670600672600674600850

PfuUltra II Fusion HotStart DNA Polymerase, 40 rxnPfuUltra II Fusion HS DNA Polymerase, 200 rxn PfuUltra II Fusion HS DNA Polymerase, 400 rxnPfuUltra II Fusion HS 2X MasterMix

0.00

5.00

10.00

15.00

20.00

25.00

PfuUltra™ IIEnzyme

Herculase® IIEnzyme

Phusion™/iProof™

DeepVent® Vent® Pfx/KOD Pfx50™ Taq

Enzy

me

Accu

racy

(x10

5 )


PCR Enzyme Fidelity Speed Yield Target length Sensitivity Page

PfuUltra II Fusion DNA Polymerase****

1 error / 2,5 million pb

**** 15 sec / kb *** ****

0-19 kb *** 4

PfuTurbo DNA Polymerase***

1 error / 770,000 pb

** 1 min / kb ***

*** 0-19 kb

(optimized)** 5

Herculase II Fusion DNA Polymerase***

1 error / 770,000 pb

**** 15 sec / kb ****

**** 0-12 >22 kb optimized

*** 7

PfuTurbo Cx Hotstart Polymerase***

1 error / 770,000 pb

** 1 min / kb *** ***

0-10 kb ** 5

PicoMaxx High-Fidelity Polymerase ** 2X Taq

** 1 min / kb **** ***

0-10 kb **** 8

Easy-A-High-Fidelity Polymerase***

1 error / 770,000 pb

** 1 min / kb *** **

0-6 kb ** 5

Paq5000 DNA Polymerase * ** 30 sec / kb ** **

0-6 kb ** 7


High Fidelity PolymerasesPfu Turbo DNA Polymerase

→ 1 min/kb from target up to 19kb (optimized)→ 1 error every 770,000 bp

600250600252600254600256

600600600602600255600257600259

PfuTurbo DNA Polymerase, 100UPfuTurbo DNA Polymerase, 500UPfuTurbo DNA Polymerase, 1000UPfuTurbo DNA Polymerase, 5000U

PfuTurbo Hotstart PCR MasterMix, 100rxnPfuTurbo Hotstart PCR MasterMix, 400rxnPfuTurbo Alternative Detergent, 100UPfuTurbo Alternative Detergent, 500UPfuTurbo Alternative Detergent, 1000U

Pfu Turbo Cx

→ Methylation Pfu Mutant that incorporates dUTPs

600410600412600414

Easy A High-Fidelity Polymerase

→ TA/UA CLONING→ Add poly-A 3’-A tails→ Proof reading polymerase activity facilitating cloning UA/TA broad band→ Available in HS and MasterMix format

600400600402600404600640600642

Easy-A High-Fidelity PCR Cloning Enzyme, 100UEasy-A High-Fidelity PCR Cloning Enzyme, 500UEasy-A High-Fidelity PCR Cloning Enzyme, 1000UEasy-A High-Fidelity PCR MasterMix, 100rxnEasy-A High-Fidelity PCR MasterMix, 500rxn

5

PfuTurbo Cx Hotstart DNA Polymerase, 100UPfuTurbo Cx Hotstart DNA Polymerase, 500UPfuTurbo Cx Hotstart DNA Polymerase, 1000U

For Research Use Only. Not for use in diagnostic procedures.


First Generation

600380600382600384600385600387

600389600390600392600394600396

600630600632600135600136600140

600153600154600159600160600163

600320600322600324600353600355600357

PfuUltra High-fidelity DNA Plymrase, 100U PfuUltra High-fidelity DNA Plymrase, 500U PfuUltra High-fidelity DNA Plymrase, 1000UPfuUltra Alternative Detergent, 100UPfuUltra Alternative Detergent, 500U

PfuUltra Alternative Detergent, 1000UPfuUltra Hotstart DNA Polymerase, 100UPfuUltra Hotstart DNA Polymerase, 500UPfuUltra Hotstart DNA Polymerase, 1000UPfuUltra Hotstart DNA Poly AD, 1000U

PfuUltra Hotstart PCR Master Mix, 100rxnPfuUltra Hotstart PCR Master Mix, 400rxnPfu DNA Polymerase (native), 100UPfu DNA Polymerase (native), 500UPfu DNA Polymerase (native), 1000U

Cloned Pfu DNA Polymerase, 100UCloned Pfu DNA Polymerase, 500UCloned Pfu DNA Polymerase, 1000UKit, Cloned Pfu DNA Polymerase, 5000UExo - Pfu DNA Polymerase, 250U

PfuTurbo Hotstart DNA Polymerase, 100UPfuTurbo Hotstart DNA Polymerase, 500UPfuTurbo Hotstart DNA Polymerase, 1000UCloned Pfu DNA Polymerase AD, 100UCloned Pfu DNA Polymerase AD, 500UCloned Pfu DNA Polymerase, 1000U



High Yields Polymerases

Herculase II Fusion DNA Polymerase

→ ROBUST for difficult samples→ Recommended in NGS protocols→ Suitable for GC-rich samples: 84%→ 15-30 sec/kb→ Target up to 12kb and 23kb if optimized

600675600677600679

Herculase II Fusion DNA Polymerase, 40rxnHerculase II Fusion DNA Polymerase, 200rxn - dNTPS includedHerculase II Fusion DNA Polymerase, 400rxn - dNTPS included

GC-Rich content

Routine PolymerasesPaq5000 DNA polymerase→ Alternative to Taq polymerase PCRs for ROUTINE→ Available in HotStart and MasterMix format→ < 5cts/U !

600680600680600684600860600862600864600870600872

Paq5000 DNA Polymerase, 500UPaq5000 DNA Polymerase, 1000UPaq5000 DNA Polymerase, 5000UPaq5000 Hotstart DNA Polymerase, 500UPaq5000 Hotstart DNA Polymerase, 1000UPaq5000 Hotstart DNA Polymerase, 5000UPaq5000 2X MasterMix, 100rxnPaq5000 2X MasterMix, 400rxn

7

Seconds / kb 15 30 45 60 15 30 45 60 15 30 45 60 15 30 45 60

Herculase IIPolymerase Phusion™/ iProof™ Pfx50™ Pwo SuperYield

6 kb

1.7 kb

Seconds / kb 15 30 45 60 15 30 45 60 15 30 45 60 15 30 45 60

Herculase IIPolymerase

Phusion™/ iProof™ Pfx50™ Pwo SuperYield

A

B



First Generation Polymerases

600260600262600264600266600310600312600314600195600196600197600198600210600211600212600280600282600284

Herculase Enhanced DNA polymerase, 100UHerculase Enhanced DNA polymerase, 500UHerculase Enhanced DNA polymerase, 1000UHerculase Enhanced DNA polymerase, 5000UHerculase Hotstart DNA polymerase, 100UHerculase Hotstart DNA polymerase, 500UHerculase Hotstart DNA polymerase, 1000UTaq2000 DNA Polymerase, 100UTaq2000 DNA Polymerase, 500UTaq2000 DNA Polymerase, 1000UTaq2000 DNA Polymerase, 5000UTaqPlus Precision PCR System, 100UTaqPlus Precision PCR System, 500UTaqPlus Precision PCR System, 1000USureStart Taq DNA Polymerase, 100USureStart Taq DNA Polymerase, 500USureStart TAq DNA Polymerase, 1000U

Herculase and Taq Polymerases

dNTPs, PCR Enhancers and Buffers200415

200435200532600129600130600147600201600420600422600424600650200339200409

Deoxynucleotides Mix PCR Grade400 μL of 100 mm dNTP mix (25 mm of each dNTP)10x Taq DNA polymerase Buffer, 10mL10x Cloned Pfu DNA polymerase Buffer, 10mLPerfect Match PCR Enhancer, 100UPerfect Match PCR Enhancer, 200UTaq Extender PCR ADditiveSingle-Stranded DNA Binding Protein, 100μgPicoMaxx High Fidelity PCR System, 100UPicoMaxx High Fidelity PCR System, 500UPicoMaxx High Fidelity PCR System, 1000UPicoMaxx High Fidelity PCR MasterMix, 100rxnRNAMaxx High Yield Transcription KitPCR Polishing Kit



Modification EnzymesRNace-it Ribonuclease Cocktail

→ RNA Degradation in oligoribonucleotides→ Mix of RNaseA and non specific RNase from Aspergillus oryzae

300140300151300152500402600011600031600032600111200410

Proteinase K, 100 mgRNase Block, 4,000 URNase Block, 16,000 UDpn I Restriction Enzyme, 200 UT4 DNA Ligase, 300 U RNase Free DNase I, 1000 URNase Free DNase I, 5000 U T3 RNA polymerase, 5,000UKlenow Fill-in Kit, 25 rxn

SureDirect Blood PCR

Ladders and luminescent stickers

400720 RNace-It Ribonuclease Cocktail, 600 μL

300140300151300152500402600011600031600032600111200410

Proteinase K, 100 mgRNase Block, 4,000 URNase Block, 16,000 UDpn I Restriction Enzyme, 200 UT4 DNA Ligase, 300 U RNase Free DNase I, 1000 URNase Free DNase I, 5000 U T3 RNA polymerase, 5,000UKlenow Fill-in Kit, 25 rxn

201100201115

PhiX174/Hae III Marker, 20 μgKb DNA Ladder (250 pb - 12k), 20μg

Direct PCR from Blood without extraction step

→ Fresh blood, dried, human or animal→ NO extraction steps even in presence of anticoagulants→ GC-rich content up to 77%→ Multiplexing and controls included

600920600930

SureDirect Blood PCR kit, 100 rxnSureDirect Blood PCR kit, 5 x 100 rxn



RT-PCR Applications

Reverse-Transcriptase Enzymes Decision Tree

RT-PCR Enzyme Fidelity Yield cDNA Length Sensitivity Page

AccuScript Reverse Transcriptase

**** 1 error / 1500 to

30,000 nt during cDNA synthesis

*** ****0-20kb *** 13

AffinityScript Multi-Temperature RT

(37-55ºC)

***1 error / 1500 nt **** ****

0-20 kb

**** (up to 1ng of total RNA)

13

10

RNA

Accuracy

AccuScript High-Fidelity RTAccuScript/Pfu Ultra II RT-PCR Kit

RobustnessYield

AffinityScript Multi-Temperature RTAffinityScript OneStep RT-PCR Kit

Lower Sample

cDNA Synthesis KitqPCR cDNA Synthesis Kit



AccuScript RTHigh Fidelity RT

→ High Fidelity and Sensitivity: Detection from 0,1ng of RNA→ Proofreading Activity→ Amplification from 1 to 20kb

600089600090

AccuScript Reverse Transcriptase, 50rxnAccuScript Reverse Transcriptase, 200rxn

AffinityScript RTRobust RT

→ Yield and Sensitivity→ Robust, GC-rich samples→ Multi-temperaturefrom 37ºC to 55ºC→ Linear Detection from 1ng to 3mg of total RNA

Add Poly-A Tails

600105600107600109

AffinityScript Multi-Temperature Reverse Transcriptase, 10rxnAffinityScript Multi-Temperature Reverse Transcriptase, 50rxnAffinityScript Multi-Temperature Reverse Transcriptase, 200rxn

600182 EASY A 1-tube RT-PCR Kit

RT and cDNA Synthesis Kits

600180600184600188

AccuScript High Fidelity RT-PCR Kit, 50rxnAccuScript PFuUltra II Rt-PCR kit, 50rxnAffinityScript Herculase II RT-PCR Kit One-Step, 100rxn

200436200820600559

AffinityScript Multi-Temperature cDNA Synthesis Kit, 50rxnAccuScript High Fidelity 1st Strand cDNA Synthesis Kit, 50rxnAffinityScript qPCR cDNA Synthesis Kit, 50rxn

600084 MMLV RT 10000U



qPCR Applications

qPCR Decision Tree



Brilliant III: Accelerate your results!Be Brilliant

→ Robust and sensitive Mastermix→ Mode FAST on all instruments: 40 cycles in less than 40 min!→ Mutant enzyme FAST-Taq DNA polymerase®: speed, strength and performance→ Hotstart Technology: Avoid primer-dimer formation→ Available in format Probe SYBR Green With or Without Rox: Low Rox, High Rox q-RT-PCR One step / Two steps

→ Flexible: Compatible with most instruments on the market ABi Real-Time PCR Systems: 7500 (FAST) ;7900 (HT) and StepOne (Plus) Viia7 Real-Time PCR System BioRad Cfx-96 System Rotor-Gene System Roche Light Cycler 480 and LC-96 Systems Agilent Mx 300(5)P System and Agilent AriaMx qPCR System

Brilliant III Ultra-Fast SybrGreen MasterMixSybrGreen Reagents

→ SybrGreen Rox separated→ SybrGreen High Rox→ SybrGreen Low Rox

600882600883

Brilliant III Ultra-Fast SYBR-Green QPCR MasterMix, 400rxnBrilliant III Ultra-Fast SYBR-Green QPCR MasterMix , -10pk, 10x400rxn

600889600904

Brilliant III Ultra-Fast SYBR-Green QPCR High ROX MasterMix, 400rxnBrilliant III Ultra-Fast SYBR-Green QPCR High ROX MasterMix, -10pk 10x400rxn

600892600903

Brilliant III Ultra-Fast SYBR-Green QPCR Low ROX MasterMix, 400rxnBrilliant III Ultra-Fast SYBR-Green QPCR Low ROX MasterMix, -10pk 10x400rxn



Brilliant III Ultra-Fast MasterMixesProbes-based Reagents

→ Probes Rox separated→ Probes High Rox→ Probes Low Rox

600880600881

Brilliant III Ultra-Fast QPCR MasterMix, 400rxnBrilliant III Ultra-Fast QPCR MasterMix , -10pk, 10x400rxn

600888600904

Brilliant III Ultra-Fast QPCR High ROX MasterMix, 400rxnBrilliant III Ultra-Fast QPCR High ROX MasterMix, -10pk 10x400rxn

600892600903

Brilliant III Ultra-Fast QPCR Low ROX MasterMix, 400rxnBrilliant III Ultra-Fast QPCR Low ROX MasterMix, -10pk 10x400rxn

Brilliant HRM Ultra-Fast LociHigh Resolution Melt Reagents

→ Stratagene Technology Mix and Go in ONE tube : no cross-contamination→ Robust and stable: multiple thawing→ Reproducible and standardized: detection of Tm changing <0,2°C→ Samples directly usable (gels, sequencing,…)→ Non toxic Dye EvaGreen→ FAST: Results in 30 to 100 min depending on instruments→ Applications Mutation screening : repeated bases, micro-micro-deletion or insertion Species identification Haplotypes characterization Fingerprints and DNA profiles Methylated DNA analysis HLA typing Compatibility Allelic differentiation: Type III and IV SNP

5190-78275190-79095190-7702

Master Mix Brilliant HRM Ultra-fast loci, 200 rxnMaster Mix Brilliant HRM Ultra-fast loci, 15 rxnAriaMx HRM Calibration Kit

SNP Class Base Change Typical change in Tm Occurrence in

human genomeI C/T and G/A Large (<0.5 ºC) 64%

II C/A and G/T Large (<0.5 ºC) 20%

III C/G Small (0.2 - 0.5 ºC) 9%

IV A/T Very Small (<0.2 ºC) 7%



qPCR Multiplex Reagents→ Multiple targets detection: amplification up to 5 in ONE reaction→ Accurate quantification in high and low abundance in a single tube→ Economic: less samples, less reagents for 5 tests 16

One step: Extraction and DetectionmiRNA Solutions

→ miRNA Extraction Kit <30 min→ miRNA q-RT-PCR Kit Detection from 15ng Sensitive : 100 copies

600036400814200344600545600580600583600564600750600037

miRNA 1st Strand Synthesis Kit, 50rxnAbsolutely RNA miRNA Kit, 50prepsmiRNA Extraction Kit, 100 à 500prepsHigh-Specificity miRNA QPCR Kit, 200rxnmiRNA Q-RT-PCR Kit, 200rxnmiRNA QPCR MasterMix, 200rxnmiRNA Q-RT-PCR MasterMix Kit, 200rxnmiRNA Forward Primer Human U6Universal Reverse Primer

600553 Brilliant Multiplex QPCR MasterMix, 200rxn

Equivalent performance with 4 target-multiplex

Q-RT-PCR Method to Detect miRNA



Alient q-RT-PCR Inhibitor Alert→ RNA external control→ Detection of PCR inhibitorsCan be amplified both and alone in presence of the interest RNA→ Available in GenBank→ An increase of Ct indicates presence of inhibitors

300600300602300604

Alien Q-RT-PCR Inhibitor AlertAlien QPCR Reference Standard (FAM) kitAlien QPCR Reference Standard (VIC) kit

Old Generation of MasterMix Brilliant II600825600826600828600829600830600831

Brilliant II SYBR Green QRT-PCR MasterMix 1-Stp10pkBrilliant II SYBR Green QRT-PCR MasetMix 1-Stp10pkBrilliant II SYBR Green QPCR MasterMixBrilliant II SYBR QPCR High ROX MasterMixBrilliant II SYBR QPCR Low ROX MasterMixBrilliant II SYBR Green QPCR Master Mix 10pk

600804600805600806600815

Brilliant II QPCR Master Mic, 400rxnBrilliant II QPCR High ROX Master MixBrilliant II QPCR Low ROX Master MixBrilliant II QPCR Master Mix 10-pk, 10x400rxn

Brilliant IIIqRT-PCR Reagents

→ SybrGreen qRT-PCR→ Probe qRT-PCR

Rox separated ONLY

600886600887

600884600885

Brilliant III Ultra-Fast SYBR-Green Q-RT-PCR MasterMix, 400rxnBrilliant III Ultra-Fast SYBR-Green Q-RT-PCR MasterMix, -10-pk, 10 x 400rxn

Brilliant III Ultra-Fast Q-RT-PCR MasterMix, 400rxnBrilliant III Ultra-Fast Q-RT-PCR MasterMix, -10-pk, 10 x 400rxn



Side Step Technology→ FAST before q(RT)PCR stepsNo extraction stepsArchiving cell cultures and RNA banks→ Lysis and stabilization Buffer Stable cell lysate > 20 months at -80°C RNA > 6 months at -20°C

Brilliant IISybrGreen→ Rox separated→ Rox integrated

600825600826600834600835600836

Brilliant II SYBR-Green Q-RT-PCR MasterMix - 1-step, 400rxnBrilliant II SYBR-Green Q-RT-PCR MasterMix - 1-step, 10-pk, 10 x 400rxnBrilliant II SYBR-Green Q-RT-PCR MasterMix AffinityScript - 3-steps, 400rxnBrilliant II SYBR-Green Q-RT-PCR MasterMix - Low Rox, 400rxnBrilliant II SYBR-Green Q-RT-PCR MasterMix - High Rox, 400rxn

Probes-based→ Rox separated→ Rox integrated

600809600818600827600810600819600837600838

Brilliant II Q-RT-PCR MasterMix - 1-step, 400rxnBrilliant II Q-RT-PCR MasterMix - 1-step, 10-pk, 10 x 400rxnBrilliant II Q-RT-PCR MasterMix AffinityScript - 3-steps, 400rxnBrilliant II Q-RT-PCR Core Kit - 1-step, 400rxnBrilliant II Q-RT-PCR Core Kit - 1-step, 10-pk, 10 x 400rxnBrilliant II Q-RT-PCR MasterMix - 1-step Low Rox, 400rxnBrilliant II Q-RT-PCR MasterMix - 1-step High Rox, 400rxn

400902400916400917400900

Kit SideStep mRNA Enrichment, 10prepsKit SideStep II Cell Lysis AnalysisSideStep II Q-RT-PCR Master Mix, 1-stepBuffer SideStep Lysis and Stabilization, 10mL



AriaMxqPCR plastics and consumables

Mx300(5)P

401266401333401334401411401421401425401427401428401447410082401490401491401492401493401494410086410088410090410091410092410094410096410098410099410152410186410187410188600536600567

1590-77025190-7708

Light blocking pad for 96-well plates96-well plates QPCR, non-skirted, pqt/2596-well plates QPCR, semi-skirted, pqt/25Mx3000/5P qPCR System Bulb Replacement AssemblyMxP Perfect Fit FrameMx3000P Optical Strip Caps (120 count)Mx3000P Optical Strip Caps (60 count)Mx3000P Strip Tubes (120 count)Mx3000P, OPTICS BOX ASSAYTube Strips, 200 μl, 80 strips of 1296-well plates AriaMx, skirt, Low Profile96-well plates AriaMx, rigid, skirtAdhesive Seal AriaMxAriaMx low profile strip tubes for PCR and qPCR applicationsOptical 96-well plates AriaMxTube Cap Strips, 80 strips of 12Polypropylene 96-Well Tube Plates,25Thin-Wall Tubes, 600 μl, 1,000 tubesThin-Wall Tubes, 200 μl, 1000 tubesTube Strips, 200 ul, 120 strips of 8Benchtop Rack for 200μl Tubes/V Bottom PlatesTube Cap Strips, 120 strips of 840-Well Working RackTube-Strip Capping Tool96-Well Plate Sealing Film, 100 sealsAdhesive Seal, 100 sealsCompression Mats, 10 mats384-well plates, 50 platesPassive Reference Dye, 1mm (10x100μl)Validation plate for Mx3000/5P, β-actinHRM Calibration plate for AriaMxSYBR qualification plate for AriaMx



Mycoplasmas detection kits→ FAST : < 30 min→ PCR or qPCR detection→ Detection of the 8 most common species

302008302106302107302108302109

Mycoplasma Plus PCR Primer Set, 50rxnMycoSensor QPCR Assay Kit, 100rxnMycoSensor QPCR Assay Kit, 50rxnMycoSensorPCR Assay Kit, 100rxnMycoSensor PCR Assay Kit, 50rxn

Universal reference RNAReference RNA

740000740100740200750500750600750700

Universal Human Reference RNA 2x200 μgUniversal Mouse Reference RNAUniversal Rat Reference RNAQuantitative PCR Human REference Total RNAStratagene QPCR Mouse Reference Total RNAUniversal miRNA Reference Kit

Total RNA

736001736005736007736009736011736019736023736501736507736509736511736513736519736521737001737005737007737009737011737013737023737101

Total RNA, Brain, Mouse, BALB/c, MaleTotal RNA, Heart, Mouse, BALB/c, MaleTotal RNA, Kidney, Mouse, BALB/c, MaleTotal RNA, Liver, Mouse, BALB/c, MaleTotal RNA, Lung, Mouse, BALB/c, MaleTotal RNA, Spleen, Mouse, BALB/c, MaleTotal RNA, Testes, Mouse, BALB/c, MaleTotal RNA, Brain, C57BL, MaleTotal RNA, Kidney, C57BL, MaleTotal RNA, Liver, C57BL, MaleTotal RNA, Lung, C57BL, MaleTotal RNA, Skeleton Muscle, C57BL, MaleTotal RNA, Spleen, C57BL, MaleTotal RNA, Testes, C57BL, MaleTotal RNA, Brain, Sprague-Dawley Rat, MaleTotal RNA, Heart, Sprague-Dawley Rat, MaleTotal RNA, Kidney, Sprague-Dawley Rat, MaleTotal RNA, Liver, Sprague-Dawley Rat, MaleTotal RNA, Lung, Sprague-Dawley Rat, MaleTotal Skltl Muscle, Sprague-Dawley Rat, MaleTotal RNA, Testes, Sprague-Dawley Rat, MaleTotal RNA, Brain, Cerblm, Fischr344 Rat, Male, 50μg



Reference RNA

540013540017540019540021540023540025540029540031540035540037540039540041540043540045540047540049540051540053540055540057540059540061540063540065540067540069540071540073540075540077540078540079540081540083540084540085540087540089540091540093540095

Total RNA, Kidney, Human, 25μgTotal RNA, Liver, Human, 25μgTotal RNA, Lung, Human, 25μgTotal RNA, Lymph Node, Human, 25μgTotal RNA, Pancreas, Human, 25μgTotal RNA, Placenta, Human, 25μgTotal RNA, Skeletal Muscle, Human, 25 μgTotal RNA, Skin, Human, 25μgTotal RNA, Spleen, Human, 25μgTotal RNA, Stomach, Human, 25μgTotal RNA, Thyroid, Human, 25μgTotal RNA, Stomach, Diseased, Human, 25μgTotal RNA, Uterus, Human, 25μgTotal RNA, Breast, Human, Female, 25μgTotal RNA, Larynx, Human, 25μgTotal RNA, Testes, Human, 25μgTotal RNA, Vulva, Human, 25μgTotal RNA, Brain Stem, Human, 25μgTotal RNA, Omentum, Human, 25μgTotal RNA, Fallopian Tube, Human, 25μgTotal, Larynx, Squamous Cell Crcnma, Human, 25μgTotal RNA, Stomach, Matched Set (Normal + Adenocarcinoma), Human, 25µgTotal RNA, Prostate, Hyperplsia, Hmn, 25μgTotal, Ileum, Non Hodgkins Lymphma, Human, 25μgTotal, Ileum, Matched Set (Normal + Non Hodgkins), Human, 25μgTotal RNA, Rectum, Human, 25μgTotal RNA, Ovary, Human, 25μgTotal RNA, Cervix, Human, 25μgTotal RNA, Larynx, Matched Set (Normal + Squamous Cell Carcinoma), Human, 25 µgTotal, Colon, Matched Set (Normal + Adenocarcinoma), Human, 25μgTotal RNA Colon Matched SetTotal RNA, Colon, Adenocarcinoma, Human, 25μgTotal RNA, Kidney, Renal Cell Carcinoma, Human, 25μgTotal Kidney, Matched Set (Normal + Renal Cell Carcinoma) Human, 25μgTotal RNA Kidney Matched SetTotal RNA, Bladder, Carcinoma,Hmn, 25μgTotal RNA, HeLa-S3 Cells, Human, 25μgTotal RNA, HL-60 Cells, 25μgTotal RNA, RAJI Cells, 25μgTotal RNA, MDA-MB-453 Cells, 25 µgTotal RNA, PC-3 Cells, 25μg



540097540099540101540103540105540107540109540111540113540115540117540121540123540125540127540131540133540135540137540141540143540145540147540149540153540157540161540165540169540173540177540181540185540187540191540193540195540197540199540201

Total RNA, HeLaS3 Cells/PMA Treated, 25μgTotal RNA, K-562 Cells/PMA Treated,25μgTotal RNA, RajiCells/PMA Treated,25μgTotal RNA, K-562 Cells, 25μgTotal RNA, U937 Cells, 25μgTotal RNA, Jurkat Cells, 25μgTotal RNA, Jurkat, Human, Ionomycin Treatd Cell, 25 µgTotal RNA, Jurkat, Human, Ionomycin/PMA treated cell, 25μgTotal RNA, HeLa S3 +/-PMA, 25μgTotal RNA, K562 +/-PMA, 25μgTotal RNA, Brain, Frontal Cortex,Hmn,25μgTotal RNA, Caval Vein, Human, 25μgTotal RNA, Colon, Ascending, Human, 25μgTotal RNA, Colon, Descending, Human, 25μgTotal RNA Parotid, Human, 25μgTotal RNA, Duodenum, Human, 25μgTotal RNA, Adrenal Gland, Human, 25μgTotal RNA, Brain, Striatum, Human, 25μgTotal RNA, Brain, Occipital Cortex, Hmn, 25μgTotal RNA, Thymus, Human, 25μgTotal RNA, Brain Parietal Cortx,Hmn, 25μgTotal RNA, Trachea, Human, 25μgTotal RNA, Penis, Human, 25μgTotal RNA, Tongue, Human, 25μgTotal RNA, Fetal Bladder, Human, 25μgTotal RNA, Fetal Brain, Human, 25μgTotal RNA, Fetal Colon, Human, 25μgTotal RNA, Fetal Heart, Human, 25μgTotal RNA, Fetal Kidney, Human, 25μgTotal RNA, Fetal Liver, Human, 25μgTotal RNA, Fetal Lung, Human, 25μgTotal RNA, Fetal Skeletal Muscle,Hmn,25μgTotal RNA, Fetal Skin, Human, 25μgTotal RNA, Fetal Spleen, Human, 25μgTotal RNA, Fetal Stomach, Human, 25μgTotal RNA, Fetal Aorta, Human, 25μgTotal RNA, Fetal Thymus, Human, 25μgTotal RNA, RAJI +/-PMA, 25μgTotal RNA, Carotid AteryHuman 25μgTotal RNA, MVP Umbilical Cord, Human, 25μg

Reference DNA201190 Salmon Sperm DNA, 10 x 1 mL



Molecular Biology Competent Cells

22

Cells Decision Tree

What is your transformation goal?



XL1 and XL2 - Blue StainsRoutine Cells

→ Blue/White colonies→ Ideal for routine cloning 5.106 to 5.109 cells→ Plasmid and vector optimal propagation→ Available formats: efficiencies of transformation, chemo-competent or electro-competent

200150

200130

200228

200236

200249

230248

XL2-Blue Ultra-competent Cells, 10 x 0,1mLHighest cloning efficiency, > 5 x 109 transformants/μgXL1-Blue Subcloning Grade Competent Cell, 8 x 0,5mLFor cloning where DNA is not limited, ≥ 1 x106 transformations/μgXL1-Blue Electro-Comp Cells, 5 x 0,1mLFor cloning unmethylatedDNA, ≥1 x 1010transformants/μgXL1-Blue Super competent Cells, 5 x 0,2mLFor high-efficiency cloning, ≥1 x 109 transformants/μgXL1-Blue CompetentCells 5 x 0,2mLFor routine cloning, ≥ 1 x 108 transformants/μgRoutine Cloning Competent Cell Pack

Classical Strains→ E-Coli Strains

200231200232200233200234200235900800

SCSI Super Competent Cells 5 x 0,2mL-1 x 109 transformants/μgAG1 Competent Cells 5 x 0,2mL-1 x 108 transformants/μgNM522 Competent Cells 5 x 0,2mL-1 x 108 transformants/μgJM101 Competent Cells 5 x 0,2mL-1 x 108 transformants/μgJM109 Competent Cells 5 x 0,2mL-1 x 108 transformants/μgW3110 Electroporation Competent Cells

XL10-Gold and ElectroTen BlueUltra-competent cells

→ High transformation efficiency: 5.109 cells→ For difficult cloning, large plasmid, ligated DNA and methylated products→ Plasmids Libraries

200314

200315200317230315230247

200159

XL10-Gold UltraCompetent Cells, 5 x 0,1mLEfficiency ≥ 5 x 109 transformants/μgXL10-Gold UltraCompetent Cells, 10 x 0,1mLXL10-Gold KanR UltraCompetent Cells,10 x 0,1mLXL10-Gold BulkPack - BaylorDifficult Cloning Competent Cell PackElectro Ten-Blue Electroporation Competent CellsHighest electroporation efficiency (Hee) phenotypeUse for extremely demanding cloning≥ 3x1010transformants/μg



gDNA or methylated cDNA→ Deficiency for cloning methylated DNA Blue MRF’cells: endA- et recA- Blue MR cells: McrA-, McrCB-, McrF-, Mrr-, HsdR-

200151200138

200158

200229

200230

200248

XL2-Blue MRF’ UltraCompetent Cells, 10 x 0,1mL> 5 x 109 transformants/μgXL1-Blue MRF’ Kan Library Pack Competent Cells, 10μgXL1-Blue MRF’ Electroporation Competent Cells, 5 x 0,1mL≥ 1 x 1010 transformants/μgXL1-Blue MR SuperCompetent Cells, 5 x 0,2mL≥ 1 x 109 transformants/μgXL1-Blue MRF’ SuperCompetent Cells 5 x 0,2mL≥ 1 x 109 transformants/μgXL1-Blue MRF’ Kan SuperComp Cell 5 x 0,2mL≥ 1 x 109 transformants/μg

Non methylated DNA

200239

200247

JM110 Competent Cells, 5 x 0,2mL≥ 5 x 106 transformants/μgSCS110 Competent Cells 5 x 0,2mL≥ 5 x 1010 transformants/μg

Convenience→ High efficiency competent cells in convenient package designed to meet with high speed requirements→ Custom solutions available

200324

230325

230350

96Pack Gold Competent Cells> 1 x 108 transformants/μgSoloPack Gold Competent Cells> 1 x 108 transformants/μg SoloPack Gold Super-Competent Cells≥ 1 x 106 transformants/μg

Random Mutagenesis200129

XL1-Red Competent Cells 5 x 0,2mLFor random mutagenesis, 1 x 106 transformants/μg



SURE Competent CellsUnstable clones

→ Deficiency in genes involved in rearrangements and deletions McrA-, McrCB-, McrF-, Mrr-, HsdR- Endonuclease endA- Recombinase recB et recJ→ Best efficiency of cloning in prokaryotes cells with irregular structures

Stop Unwanted Rearrangement Events

200152

200238

200227230247

SURE 2 SuperCompetent Cells, 10 x 0,1mL> 1 x 109 transformants/μgSURE Competent Cells, 5 x 0,2mL> 1 x 108 transformants/μgSURE Electro-Competent Cells, 5 x 0,1mL> 1 x 1010 transformants/μgDifficult Cloning Competent Cell Pack

ABLE Competent CellsToxic clones

→ ABLE C reduced ≈ 4x the number of vector copies→ ABLE K reduced ≈ 10x the number of vector copies

ABLE Electroporation Competent Cells Kit, 5 x 0,1mLIncludes both ABLE C and KABLE C Electroporation Competent Cells, 5 x 0,1mLABLE K Electroporation Competent Cells, 5 x 0,1mLABLE Competent Cells Kit, 5 x 0,2mLIncludes both ABLE C and KABLE C Electroporation Competent Cells, 5 x 0,2mLABLE K Electroporation Competent Cells, 5 x 0,2mLDifficult Cloning Competent Cell Park

200160

200161200162200170200171

200247200172

Phage Display Libraries→ Production of 1.1010 transformants/μg of DNA

TG1 Electro-Comp Cells 5 x 0.1 mL200160



© Agilent Technologies, Inc. 2015

Proteins Expression

→ High efficiency of transformation→ Direct cloning for almost all constructions→ Phenotype (Hte) and endA inactive→ BL21-DE3 Line derived: T7RNA polymerase gene controlled by the lacUV5 promoter→ BL21-DE3-pLys Line derived: PLysS plasmid encoding the T7 lysozyme, natural inhibitor of T7 RNA Pol

BL21 Cell Line

200131200132200133230130230132230134235200230246

BL21(DE3) Competent Cells 5 x 0.2 mLBL21(DE3) pLysS Comp Cells5 x 0.2 mLBL21 CompetentCells5 x 0.2 mLBL21-Gold Competent CellsBL21-Gold (DE3) Competent CellsBL21-Gold (DE3) pLysS CompCells 10 x 0.1mLKit, Lambda CE6 InductionProtein Expression Competent Cell Pack

Codons Bias→ Copies of additional tRNA→ Difficult proteins expression RIL strain for AT-rich genome RP strain for GC-rich genome

230240230245230250230255230265230275230280235200230246

BL21-CodonPlus -RIL Competent CellsBL21-CodonPlus (DE3)-RIL Comp CellsBL21-CodonPlus -RP Competent CellsBL21-CodonPlus (DE3)-RP Comp CellsBL21-CodonPlus (DE3)-RIL-X Comp CellsBL21-CodonPlus (DE3)-RP-X Comp CellsBL21-CodonPlus (DE3)-RILP Comp CellsKit, Lambda CE6 InductionProtein Expression Competent Cell Pack

Codons Bias→ Cells derived from BL21 used with: pET, pCAL or other vector containing T7RNA promoter

200124200134

TKX1 Competent Cells 5 x 0.2 mLTKB1 Competent Cells 5 x 0.2 mL



ArcticExpress Cell Line

Expression of insoluble proteins

→ Derived from BL21 Gold→ Low growth temperature improving folding and protein solubility→ Cpn60 et Cpn10 Proteins chaperones from Oleispira antarctica

AdEasy Competent Cells

BJ5183 Elextroporation-Competent CellsBJ5183-AD-1 Electroporation Comp Cells

200154200157

230191230192

ArcticExpress Competent CellsArcticExpress (DE3) Competent Cells

Codons Bias

Combination of technologies BL21 CodonsPlus Adaptation to low growth temperatures→ RIL strain for AT-rich genome and RP strain for GC-rich genome

230193230194230195230196

ArcticExpress (DE3) RIL Competent CellsArcticExpress (DE3) RP Competent CellsArcticExpress RIL Competent CellsArcticExpress RP Competent Cells




Molecular Biology Cloning

StrataClone Kits

→ In 5 min at room temperature→ Available in «blunt-end» and «UA cloning»→ Yield >95% of positive inserts→ Compatible with amplicons from 500pb to 9,2kb→ Combination of DNA topoisomerase I and recombinase Cre

StrataClone PCR Cloning Kits

240205240207240218

StrataClone PCR Cloning Kit, 20rxnStrataClone Blunt PCR Cloning Kit, 20rxnStrataClone Ultra Blunt PCR Cloning Kit, 20rxn

StrataClone Mammalian

→ Cloning in 5 min→ Entire experiment <1 jour→ Fast protein detection via tag epitopes FLAG®

c-Myc→ Tags don’t interfere with the protein

240228240229240230240231240232

StrataClone Mammalian Untagged, 20rxnStrataClone Mammalian N-Flag, 20rxnStrataClone Mammalian C-Flag, 20rxnStrataClone Mammalian N-cMyc, 20rxnStrataClone Mammalian C-cMyc, 20rxn

PCR Polishing Kit→ Best alternative to Klenow Polishing→ Generating DNA fragment with Blunt-End extremity >30min

200409 PCR Polishing Kit



Libraries and cloning

→ 66 different sources of mRNA→ Complete collection of human transcriptome in a single tube→ cDNA pool enrichment protocol→ Cloning of genes and identified transcripts with EST tag→ Size fractionation Small fragments ≈ 800bp Large fragments > 2000bp→ Each fragment is cloned separately

MegaMan Human Transcriptome

790000790001

MegaMan Human Transcriptome Libr, 20rxnMegaMan Human Transcriptome Libr, 100rxn

Libraries Packaging

→ Single tube→ Increase efficiency and bank representation→ Preservation of methylated DNA: Restrictionminus (HsdR– McrA– McrBC– McrF– Mrr–)→ Directly usable for epigenetic studies→ Efficiency up to 2.109 pfu / μg GigaPack III: Best efficiency – Bank gDNA construction GigaPack III Plus: Economical alternative – High efficiency non required GigaPack III XL: Large inserts 47-51kb – Cosmid and lambda libraries NO fractionation step

Gigapack III Packaging Extracts

200201200202200203200204200205200206200207200208200209

Gigapack III Gold Packaging Extract, 4rxnGigapack III Gold Packaging Extract, 7rxnGigapack III Gold Packaging Extract, 11rxnGigapack III Plus Packaging Extract, 4rxnGigapack III Plus Packaging Extract, 7rxnGigapack III Plus Packaging Extract, 11rxnGigapack III XL Packaging Extract, 4rxnGigapack III XL Packaging Extract, 7rxnGigapack III XL Packaging Extract, 11rxn

TransPack Packaging Extract

→ Optimized for Big Blue Rodent Assays200220200221200223

Transpack Packaging Extract, 400 rxnTranspack Packaging Extract, 100 rxnTranspack Packaging Extract, 50 rxn




Cloning Vectors

→ Prokaryotic expression (Promoter lacZ)→ Eukaryotic expression (Promoter CMV)

Lambda ZAP Vector

200251200252200253211203211204237211237612239201239211239212211199239614239615720120

VCSM13 Interference Resistant Hlpr Phg; 1mLR408 Interference Resistant Helper Phage; 1mLExAssist Helper Phage with SOLR; 1mLExAssist Helper Phage with XOLRRapid Excision KitUni-ZAP XR VectorKitKit, Uni-ZAP /Gigapack III Gold CloningZAP Express Vector KitZAP Express EcoRI/CIAP-Treated VectorZAP Express BamHI/CIAP-Treated VectorKit, pCMV-Script PCR CloningKit, ZAP Express EcoRII/GigapackIII VcKit, ZAP Express BamHI/GigapackIII VctKit, Lambda Select-cII Mutation Assay

→ Vector ZAP-CMVHigh-yield eukaryotic expression

239220239221239222239224

Lambda ZAP -CMV Undigested VectorLambda ZAP -CMV RI Cloning KitLambda ZAP -CMV XR Cloning KitKit, Lambda ZAP -CMV XR Vector Gigapack

→ Kit ZAP II: combined with pBluescript SK- vector

236201236211236612

Lambda ZAP II Vector KitLambda ZAP II / EcoR I / CIAP Treated VctrKit, Lambda ZAP II / EcoRl / Gigapack III Gol

→ Kit Lambda Genomic: Lambda DASH II and Lambda FIX II Vectors for gDNA cloning from 9 to 23kb

247201247211247212247613247614247713247714

Lambda DASH II Undigested Vector KitLambda DASH II / BamH I Vector KitLambda DASH II / EcoR I Vector KitKit, Lambda DASH II / BamH I / Gigapack IIIKit, Lambda DASH II / EcoRI / Gigapack IIIKit, Lambda DASH II / BamHI / Gigapack III XKit, Lambda DASH II / EcoRI / Gigapack III XL

248201248211248612248712241211241612241711

Lambda FIX II Undigested Vector KitLambda FIX II / XhoI Partial Fill-in VectKit, Lambda FIX II / Gigapack III CloningKit, Lambda FIX II / Gigapack III XL CloningLambda EMBL3 / BamH I Vector KitKit, Lambda EMBL3 / Gigapack III Gold CloningKit, Lambda EMBL3 / Gigapack II XL Cloning



Libraries and cloning

→ Kit Lambda gt11: libraries of cDNA constructionScreening with antibodies and probes> 1.107pfu / μg lambda DNADo not allow in vivo excision of the cloned fragments

MegaMan Human Transcriptome

234211234612

Lambda gt11 / EcoR I / CIAP Treated Vctr KitKit, Lambda gt11 / EcoR I / Gigapack III Gol

pBlueScript II Vectors→ Cloning vectors originating from phage→ Simplifying procedures Cloning and sequencing Generating in vitro transcripts Mapping Mutagenesis→ Flexibility: Extensive polylinker with 21 unique recognition sites of restriction enzymesIn 2 directions→ T3 and T7 Promoter for RNA in vitro transcription→ Resistance gene to Ampicillin→ Based on plasmid ColE1 - High copy number→ Available with the origin f1 in either direction (+) or (-)

212205212206212207212208212240212250

pBluescript II SK (+) Phagemid KitpBluescript II SK (-) Phagemid KitpBluescript II KS (+) Phagemid KitpBluescript II KS (-) Phagemid KitpBluescript II XR Predigested VectorpBluescript II RI Predigested Vector

pBC Phagemid Vectors→ Based on vector pBlueScript II→ Resistance gene to chloramphenicol

212215212216212217212218

pBCSK (+) PhagemidpBCSK (-) PhagemidpBCKS (+) PhagemidpBCKS (-) Phagemid

SuperCos I Vectors→ Cosmid Vector of 7,9kb comprising flanking sequences generating a unique cloning site→ For Fast synthesis of specific «walking probes»→ Suitable for amplification and expression of cosmid clones in eukaryotic cells→ Cloning capacity: 30 to 42kb→ Generating cosmid libraries→ Ideal for high - resolution mapping

251301 SuperCos I Vector Kit




Molecular Biology Transfection

GeneJammer Transfection Reagent→ Easy and FAST: No need to media change→ Proprietary formulation based on polyamines→ Ideal for stable and transient transfection→ Low cytotoxicity for a large number of cell lines→ Compatible with supplemented mediaAntibiotics, growth factors

Evaluation of HeLa cells viability transfected with pCMV-ß-gal

204130204131204132

GeneJammer Transfection Reagent, 1 mLGeneJammer Transfection Reagent, 4 x 1 mLGeneJammer Transfection Reagent, 0,3 mL

pCMV-beta-galactosidase were used to transfect COS-7 cells (A) and human umbilical vein endothelial cells (B)

GeneJammer Transfection Reagent→ Modified method based on CaPO4-→ Packaging Cell Line : HEK293→ High transfection efficiency for recombinant virus and insertion of viral DNA

200488 ViraPack Transfection Reagent



Mammalian Transfection Kit→ FAST and effective→ Simplified and reproducible protocol→ ≈ 100x > CaPO4-method→ Control plasmidvector: pWLneo: G418 (neomycin) selection

MBS Mammalian Transfection Kit→ Best transformant yield→ Cells incubated with CaPO4-/ DNA for only 3h→ Serum bovin modified to increase the number of transfected cells→ Multiple micro-transfections 96-well plate, up to 5000 cells/well

200285200385200388

Mammalian Transfection KitMammalian Transfection-Calcium PhosphateTransfection MBS Mammalian Kit

Chemicals

200049200399300020300021300024300127300201400510

G418 Sterile Solution, 10 mL, 50mg/mLG418 Sulfate, 1gAmpTabs Ampicillin Tablets, 200 x 2.5mgAmpTabs Ampicillin Tablets, 200 x 25mgTurboAmp Antibiotic, 10g powderIPTG, 1gX-Gal, 1gComplex Proteomics Standard




Molecular Biology Mutagenesis

Kits QuikChange→ Fast, mutagenesis < 3h and Dpn1 digestion in 5 min→ NO subcloning steps→ Insertions – Deletions – Inversions for plasmids up to 14kb→ > 80% efficiency for 1 mutation and > 55% efficiency for 3 mutations→ Free access to SureDesign software

Directed Mutagenesis

Generation I: Pfu Turbo DNA Polymerase and XL1-Blue Supercompetent CellsGeneration II: Pfu Ultra High-Fidelity DNA Polymerase and XL10 Gold Cells – from 4 to 8kbGeneration II-E: Electroporation format: XL1 Blue Electroporation competent CellsVersion II-XL: Large insert from 8 to 14kbVersion Multi: Until 5 mutation sites in ONE GO – from 4 to 8kbGeneration III Lightning: Difficult plasmids, GC-rich and large Pfu Ultra II Fusion High-Fidelity DNA Polymerase and QC Lightning Enzyme and XL10 Gold Cells

How to choose your mutagenesis kit?

200514200515200516200517200518200519200521200522200523200524200555210513210515210518210519

Kit QuikChange Multi Site-Directed, 30rxnKit QuikChange Multi Site-Directed,10rxnKit QuikChange XL Site-Directed, 30rxnKit QuikChange XL Site-Directed, 10rxnKit QuikChange Site-Directed, 30rxnKit QuikChange Site-Directed, 10rxnKit QuikChange II XL site-directed,10rxnKit QuikChange II XL site-directed, 30rxnKit QuikChange II site-directed, 10rxnKit QuikChange II site-directed, 30rxnKit QuikChange II-E Site-Directed, 10rxnKit QuikChange Lightning Multi, 30rxnKit QuikChange Lightning Multi, 10rxnKit QuikChange Lightning, 10rxnKit QuikChange Lightning, 30rxn



Kits GeneMorph II→ Method based on error-prone PCR: DNA polymerase II Mutazyme→ Equivalent mutation rate between AT and GC : uniform mutational spectrum→ Best representation of mutants libraries→ High Yield from 0,1 to 6kb→ Mutation rate of 1-16 bases/kb→ EZClone domain : Prefers mutations in protein domains and promoting elements

Random Mutagenesis

200550200552

GeneMorph II random mutagenesis kitGeneMorph II EZClone Domain kit

Big Blue→ In Vivo system to assess genetic toxicity of tested compounds→ Alternative to screening Plate-Color for mutant screening→ Cell line derived from rat embryonic fibroblast cells Rat2

In vivo mutagenesis study

Big Blue Lambda LIZ Vecteur

720150720230720300722000746220

Big Blue Lac I PCR Primer SetBig Blue Color Control platesBig Blue MediaBig Blue Maltose MgSO4Big Blue Lambda LIZ Shuttle Vector

Overview of the λ Select-cIImutation detection system35For Research Use Only. Not for use in diagnostic procedures.



Gene Editing and Synthetic Biology

QuikChange High Throughput→ FAST, easy and reliable: an efficient design reduces the cost of screening!→ Linear amplification based on Error Prone PCR→ Fully customizable solution Libraries from 100 to 120 000 sequences precisely defined by user→ Free Software : SureDesign Mutagenesis Design Workspace→ Applications Targeting and mutation of all or part of amino acids from DNA to colonies < 24h (1 to 20aa x 50 regions) Rapid Identification of functional sites by saturating the entire protein Without knowing the structure of the protein One protein or variants

High Throughput MutagenesisProtein Engineering Tools

G5902AG5902BG5903AG5903B

QuikChange HT 150nt académique, 10sitesQuikChange HT 150nt académique, 20sitesQuikChange HT 200nt académique, 10sitesQuikChange HT 200nt académique, 20sites



CRISPR - Cas System type IIGenome Engineering Tools

CRISPR-Cas9 System→ Customizable and integrated solution for in-vitro research→ Improved selectivity and flexibility compared with restriction enzymes→ Free software : SureDesign CRISPR Guide RNA Design Workspace→ Directly usable for In-vitro cloning of large fragments Complex clones libraries with very high specificity In-vitro / in-vivo transfectionKit SureGuide Cas9 Nuclease In-vitro cloning for large DNA fragments with NO limitation Blunt-end Purified recombinant Cas9 with NO NLS SureGuide gRNA in vitro expression and purification system Including all necessary reagents: controls, sgRNA templates, …Kit SureGuide gRNA Generating high quality of gRNA Simple, fast: < 2,5h Including all necessary reagents: T7, small RNA purification kit, phosphatase,…

5190-77145190-77155190-77165190-77175190-77185190-7719

Full kit SureGuide CRISPR/Cas, 40rxnSureGuide Cas9 Nuclease Kit, 20rxnSureGuide Cas9 Nuclease Kit, 100rxnSureGuide Cas9 Nuclease Kit, 100rxnSureGuide gRNA Kit de control, 20rxnSureGuide gRNA Synthesis Kit, 50rxn

Workflow in vitro


38



SureVector

SureVector SystemNext Generation Cloning Tool

→ FAST, simple, reliable solution From Design to construction <24h From Design to transformation <30 min without functionality loss!→ Assembly of multiple DNA fragments in a recombinant plasmid→ Each component is interchangeable→ Fully customizable: standard combinations or custom fragments→ Free Software: SureVector Tool→ Up to 30 adapted systems for mammalian, yeast and bacterial cells Selection arlers. replication origins de, Promoters A variety of Tag (Solubility / Affinity)

G7514AG7515AG7515BG7516A/BG7517A/BG7518AG7518BG7518CG7518DG7518E

SureVector Core Kit, 15rxnSureVector E.Coli Expansion Kit N-term, 15rxnSureVector E.Coli Expansion Kit C-term, 15rxnSureVector Mammalian Expression Kit, 15rxnSureVector Yeast Expression Kit, 15rxnSureVector E.Coli Selection Kit, 5rxnSureVector E.Coli Promoter Kit N-term, 5rxnSureVector E.Coli Promoter Kit C-term, 5rxnSureVector E.Coli Tag Kit N-term, 5rxnSureVector E.Coli Tag Kit C-term, 5rxn



Viral vectorsAdEasy Adenoviral Vector Systems

Expression Systems

→ Wide range of infectivity: Several mammalian cells lines→ DNA of interest cloned into 4 vectors pShuttle-CMV, pShuttle, pShuttle-IRES-hrGFP-1 pShuttle-IRES-hrGFRP-2→ 300% of recombinants: 107 pfu/mL after transient transfection → Large insert: 7,5kb; Infects cells dividing or not→ Expression of human proteins with post-translational modification

200154200157240005240006240007240008240009240010240081240082240085

BJ5183 Electroporation-Competent CellsBJ5183-AD-1 Electroporation Comp CellspAdEasy-1 VectorpShuttle VectorpShuttle-CMV VectorpShuttle-CMV lacZ VectorKit AdEasy Adenoviral Vector SystemKit AdEasy XL Adenoviral SystempShuttle-IRES-hrGFP-1 vectorpShuttle-IRES-hrGFP-2 vectorAD-293 Cells

→ Immunoassay to detect contamination of adenovirus→ Detection between 24 and 48h via a capsid protein: hexon

972500 AdEasy Viral Titer Kit

→ Purification kit and concentration of adenovirus(Ad5 strain) between 2 and 3h

240243 AdEasy Virus Purification Kit 2x100




ViraPort Retroviral Gene Expression→ 1-5 cDNA / cell→ Transduction efficiency ≈100% On mammalian cell lines into mitosis→ Funtional cloning and screening libraries→ Copy number controlled by the multiplicity of infection (MOI)

217561217563217564217566217567217568217569217570240027240029240030240488

pFB-Neo VectorpFB VectorpFB-ERV VectorpVPack-GP VectorpVPack-VSV-G VectorpVPack-Ampho VectorpVPack-Eco VectorpVPack-10A1 VectorVitality pFB-hrGFP Retroviral VectorpFB-Neo-LacZ Retroviral VectorpFB-Luc Retroviral VectorViraPack Transfection Reagent

AAV Helper-Free System→ Adenovirus WT unnecesary→ For a broad host range→ Dividing cells or not

240071240073240074240075240109

Kit, AAV Helper-Free SystemAAV-293 CellspAAV-hrGFP VectorpAAV-IRES-hrGFP VectorAAV-HT1080 Cells

pET Expression System→ In Vivo bacterial expression system→ Based on RNA polymerase from T7 phage selectivity→ BL21 competent cells included

211521211523211621211623

pET-3 a-d VectorspET-11a-d VectorsKit pET-3 a-d Expression systemKit pET-11 a-d Expression system

Bacterial vectors



→ pET-based Vectors System→ Unique Tags improving efficiency→ E.Coli host → Two different tags 3 Tags improving SET solubility - Solubility Enhancement Tags 1 Tag improving SBP purity and high-yield - Streptavidin Binding Peptide

240162240163240164240165240172240174240175240176240177240184240105

VeriFlex N-terminal SBP Vector SetKit, VariFlex N-terminal SBP SystemVariFlex N-Terminal SBP-SET Vector SetKit, VariFlex N-terminal SBP-SET Expressn SystemVariFlex N-Terminal SET Vector SetVariFlex C-Terminal SBP VectorVariFlex C-Terminal SBP SystemVariFlex C-Terminal SBP-SET Vector SetVariFlex C-Terminal SBP-SET Expressn SystemVariFlex C-Terminal SET Vector SetStreptavidin Resin 2.5 mL

VariFlex Bacterial System

→ pET-based Vectors System→ Derivative of the RNA polymerase of phage T7→ Easy and reliable method to express high level of protein purification

pCAL-Vector: Affinity expression and purification

pESC Yeast Epitope Tagging Vectors→ Based on Tag epitopes technology with S. cerevisiae→ Each vector contains GAL1 and GAL10 Promoters in opposite orientation T3 and T7 Promoters to generate RNA transcripts One of the 4 selection markers: HIS3, TRP1, LEU2, or URA3 Epitopes peptides Polylinker→ Introduction and expression of 2 genes simultaneously→ Quick Immuno precipitation

217451217452217453217454217455

pESC-HIS VectorpESC-LEU VectorpESC-TRP VectorpESC-URA VectorpESC Vectors, all four




StrataClone Mammalian→ Cloning in 5 min→ Entire experiment < 1 day→ Quick detection of protein via tag-epitopes FLAG®

c-Myc→ Tags do NOT interfere with protein

pCMV-Tag Vectors→ Maximizing level of expression→ Based on Tag epitopes technology to overcome specific antibodies→ Fast detection of protein via tag epitopes pCMV-Tag: 1 copy of epitope markers pCMV-3Tags: 3 copies→ Tags do NOT interfere with protein analysis

240195240196240197240198242000240202240203240204257261

211170211172211173211174211175

pCMV-3 Tag-1 Epitope Tagging MammlnExpVctrpCMV-3 Tag-2 Epitope Tagging MammlnExpVctrpCMV-3 Tag-3 Epitope Tagging MammlnExpVctrpCMV-3 Tag-4 Epitope Tagging MammlnExpVctrpCMV-3 Tag-6 vectorspCMV-3 Tag-7 vectorspCMV-3 Tag-8 vectorspCMV-3 Tag-9 vectorsAnti-c-Myc Monoclonal Antibody (Clone 9E10)

pCMV-Tag 1 VectorpCMV-Tag 2 VectorpCMV-Tag 3 VectorpCMV-Tag 4 VectorpCMV-Tag 5 Vector

Mammalian vectors

240228240229240230240231240232

StrataClone Mammalian Untagged, 20rxnStrataClone Mammalian N-Flag, 20rxnStrataClone Mammalian C-Flag, 20rxnStrataClone Mammalian N-cMyc, 20rxnStrataClone Mammalian C-cMyc, 20rxn

LacSwitch II System→ Inducible In Vivo expression system→ Based on modified lac operon and repressive CMV promoter→ Eight unique sites for directional insertion of the targeted gene

217450 LacSwitch II Mammalian Expression System



→ In vitro transient expression vectors→ Size = 4,1kb combining properties of pKCR2 vector pBS vector

213201 216201

pMC1Neo and pMC1Neo Poly A VectorspSG5 Expression Vector

pSG5 & pMC1Neo Vectors

→ Based on fast PCR cloning method < 1h→ Fifteen unique sites for directional insertion of the targeted gene

212220212224

pCMV-Script VectorpCMV-Script XR Predigested Vector

pCMV-Script Vectors

Complete control system→ Inducible receptor to synthetic Ecdysone→ Dose-sensitive system→ Induction > 1700x

240028217460217461217467217468217564

pCFB-EGSH Retroviral VectorpERV3 VectorpEGSH VectorPonasterone A, 1 mgComplete Control Vector KitpFB-ERV Vector

StrataClean Resin→ No phenol/chloroform extraction step→ Non-toxic, non-flammable Resin in suspension→ Extracted DNA Directly usable in sequencer or for ligation/digestions enzymes→ Easy and fast protocol

400714400715900775

StrataClean Resin, 3 mLKit StrataClean Resin, 9 mLStrataClean Resin, 25 mL




pDual Expression SystemDual Expression VectorspBK-CMV Phagemid Vector

→ Heterologous genes Expression in eukaryotic (mammalian) and prokaryotic systems→ Time saving: NO subcloning step

214501214502214503212209

pDual Expression VectorpDual Expression VectorpDual GC Vector 20 ug (1 ug/ul)pBK-CMV Vector

Vitality hrGFP (II) Mammalian ExpressionFluorescence

→ Humanized, Renilla reniformis Green Fluorescent Protein→ Antibodies @hrGFP do NOT interfere with Ab@GFP→ Less toxic than EGFP

240141240142240143240144240145240157240241240242240027240031240032240035240036240042240043240044240059240062240063

Vitality full length hrGFPantibdy,20μlVitality full length hrGFPantibd,100μlVitality hrGFP II-1 Mammalian Expression VectorVitality hrGFP II-C Mammalian Expression VectorVitality hrGFP II-N Mammalian Expression VectorVitality IRES-hrGFP II Mammalian Expression VectorVitality hrGFP monoclonal antibody 20 μlVitality hrGFP monoclonal antibody 100μlVitality pFB-hrGFP Retroviral VectorVitality pIRES-hrGFP-1 VectorVitality pIRES-hrGFP-2 VectorVitality phrGFP-C Mammalian Expression VectorVitality phrGFP-N Mammalian Expression VectorVitality phr-GFP-Mito Subcellular VectorVitality phrGFP-Nuc Subcellular VectorVitality phrGFP-Golgi Subcellular VectorVitality phrGFP-1 VectorVitality phrGFP Promoterless VectorVitality phrGFP-Perox Subcellular Vector



Luciferase Assay KitLuminescence

→ Luciferase Kit: fast, easy to use, sensitive and quantitative→ Luciferase Reporter from dragonfly Photinus pyralis→ Highly sensitive: ≈1.10–20 moles of luciferase→ Very low noise

Light emitted by the luciferase-catalyzed chemiluminescentreaction, in relative light units (RLU), measured 8 seconds after the addition of 0.1 ng of luciferase and every 30 seconds thereafter, with integration

periods of 1 second.

219020 Luciferase Assay Kit

LumiMax Superoxide Anion Detection Kit→ Detection of super oxide anions→ Better sensitivity than the Luminol-based Assay→ Non cyto-toxic enhancer

204525 LumiMax® Superoxide Anion Detection Kit

PathDetect Cis-Reporting Systems & PathDetect Trans-Reporting Systems→ Intracellular signaling pathways identification→ Determination of director indirect influence→ Fast, simple and sensitive→ Co-transfection of pLuc-MCS luciferase reporter→ Ability to detect an In vivo activation paths with Vitality hrGFP reporter

219031219036219040219041219042219050219053219054219056219059219062219065219068219071219073219074

PathDetect pFA-cFos PlasmidPathDetect pFA-CMV PlasmidPathDetect pMUT-ELK1 PlasmidPathDetect pMUT-cJun PlasmidPathDetect pMUT-CREB PlasmidPathDetect pFR-Luc PlasmidPathDetect pFA-Jun PlasmidPathDetect pFA-CHOP PlasmidPathDetect pFC-dbdPlasmidPathDetect pFC-MEKK PlasmidPathDetect pFA-Elk1 PlasmidPathDetect pFC-MEK1 PlasmidPathDetect pFA-CREB PlasmidPathDetect pFC-PKA PlasmidPathDetect AP-1 Cis-Reporting SystemPathDetect pAP1-Luc Plasmid

Intracellular Signaling Pathways




PathDetect TRANS PathDetect CIS

219000219001219002219004219005219010219015219075219078219080219082219084219085219086219087219088219089219090219075219077219079219081219083219091

PathDetect c-JUN Trans-Reporting SystemPathDetect pFR-CAT Trans-Reporter PlasmidPathDetect pFR-B-Gal Trans-Rporter PlasmidPathDetect pFR-SEAP Trans-Reporter PlasmidPathDetect Elk1 Trans-Reporting SystemPathDetect CREB Trans-Reporting SystemPathDetect CHOP Trans-Reporting SystemPathDetect pCRE-Luc PlasmidPathDetect pNFkB-Luc PlasmidPathDetect pSRE-Luc PlasmidPathDetect pSRF-Luc PlasmidPathDetect pFC-p53 PlasmidPathDetect p53-Luc PlasmidPathDetect pFC-MEK3 PlasmidPathDetect pLuc-MCS PlasmidPathDetect pNFAT-Luc PlasmidPathDetect pISRE-Luc PlasmidPathDetect pCIS-CK PlasmidPathDetect CRE Cis-Reporting SystemPathDetect NF-kBCis-Reporting SystemPathDetect SRE Cis-Reporting SystemPathDetect SRF Cis-Reporting SystemPathDetect p53 Cis-Reporting SystemPathDetect pGAS-Luc Plasmid

219092219093219094219095240039240049240050240051240053240111240112240113240114240115240116240119240120240129240130240131240132240133240134240135240136

PathDetect ISRE Cis-Reporting SystemPathDetect GAS Cis-Reporting SystemPathDetect NFAT Cis-Reporting SystemPathDetect TARE Cis-Reporting SystemPathDetect pTARE-Luc PlasmidPathDetect pAP-1-hrGFP Cis-Reporter PlasmidPathDetect pCRE-hrGFPCis-Reporter PlasmidPathDetect pNF-kB-hrGFP Cis-Reporting PlasmidPathDetect pNFAT-hrGFPCis-Reporter PlasmidPathDetect c/EBP Cis-Reporting SystemPathDetect c/EBP Cis-Reporting PlasmidPathDetect DR1/RXRE Cis-Reporting SystemPathDetect DR1/RXRE Cis-Reporting PlasmidPathDetect DR3/VDR Cis-Reporting SystemPathDetect DR3/VDR Cis-Reporting PlasmidPathDetect DR5/RARE Cis-Reporting SystemPathDetect DR5/RARE Cis-Reporting PlasmidPathDetect Egr-1 Cis-Reporting SystemPathDetect Egr-1 Cis-Reporting PlasmidPathDetect LILRE Cis-Reporting SystemPathDetect LILRE Cis-Reporting PlasmidPathDetect GRE Cis-Reporting SystemPathDetect GRE Cis-Reporting PlasmidPathDetect DR4/TR Cis-Reporting SystemPathDetect DR4/TR Cis-Reporting Plasmid



InterPlay Mammalian TAP SystemProtein Interactions

→ Combination of two systems TAP: Tandem Affinity Purification Gene Delivery System: AdEasy Adenoviral Vector System→ Wide range of mammalian cells dividing or not→ High yield of proteins→ Expression of interest protein fused to two affinity tags A linker peptide to Streptavidine: SBP A linker peptide to Calmoduline: CBP Interest protein and endogenous interaction analysis

240106240105240099240101240102240103240104240107240213240214240215240216240245240243240244972500

MS-Grade Calmodulin Resin 0,625 mLStreptavidin Resin 2,5mLInterPlay TAP Purification Buffer KitInterPlay N-terminal Mammalian TAP VectorsInterPlay C-Terminal Mammalian TAP VectorsKit, InterPlay N-Terminal Mammalian TAP SystemKit, InterPlay C-Terminal Mammalian TAP SystemInterPlay Mammalian TAP Purfication KitInterPlay Adenoviral N-Terminal TAP SystemInterPlay Adenoviral N-terminal TAP VectorInterPlay Adenoviral C-Terminal TAP SystemInterPlay Adenoviral C-Terminal VectorsAdEasyVirus Purification Kit, 2x100; 1 prepAdEasyVirus Purification Kit, 2x100; 2 prepsAdEasyVirus Purification Kit, 2x100; 5 prepsAdEasyViral Titer Kit

Antibodies

Cold racks

200471200472200473200474

Anti-FLAG M2 Monoclonal Antibody, 200 μgAnti-FLAG M2 Monoclonal Antibody, 1 mgRAT Anti-DYKDDDDK Tag Monoclonal Antibody, 200 μgRAT Anti-DYKDDDDK Tag Monoclonal Antibody, 1 mg

400005401349

StrataCooler Cryo Presevation ModuleStrataCooler LP, Blue




MARS ColumnsImmunoDepletion: Multiple Affinity Removal SPin Cartridges

Reagents & mRP2

5188-5254

5185-5986

5186-59875185-5988

5185-59955188-52525188-5249

5185-59905188-52515188-52505188-5253

Spin Cartridge Reagent KitContains 1x Buffer A, 1x Buffer B, 2x 0.22μm spin filters, 1x 5KDa

MWCO 4 mL spin concentrators, 1x Luer lock cap adapters, 1x Plastic luer lock syringes, 6x 1.5mL μtubes with caps, 1x Spin

cartridge caps and plugs, 1x Teflon luer lock needle

Reagent Kit for MARS columnContains 2x Buffer A, 1x Buffer B, 2x 0.22μm spin filters, 1x

5KDa MWCO 4 mL spin concentrators

Buffer A - Equil / Load / Wash, 1LBuffer B - Elution 1L

End Fitting with 2um Frit, PEEK, 1ea (for MARS column)Spin Cartridge Caps and Plugs, pk/6 eachLuer-Lock Adapters for Spin Cart., pk/2

Filters, Spin, 0.22um, CellAcet, pk/25Microtube, Screwtop, 1.5mL, pk/100

Syringes, Plastic, 5mL, Luer-Lock, pk/2Needles, Teflon, Luer-Lock, pk/10

Reagents

Fittings

Other accesories

DescriptionReferences



MARS Columns

5188-53345188-65625188-88255188-88265188-52175188-52185188-52895185-59845185-59855188-53325188-53335188-53365188-52305188-53415188-64095188-64105188-64115188-64085188-65575188-65585188-65595188-6560

ReferencesSpin-cartridge

ColumnSpin-cartridge

ColumnColumnColumn

Spin-cartridgeColumnColumnColumnColumnColumn

Spin-cartridgeSpin-cartridge

ColumnColumnColumn

Spin-cartridgeColumnColumnColumn

Spin-cartridge

Type0.45mL

4.6 x 50mm0.45mL

4.6 x 50mm4.6 x 50mm4.6 x 100mm

0.45mL4.6 x 50mm4.6 x 100mm

4.6 x 50mm HC4.6 x 100mm HC10 x 100mm HC

0.45 mL0.45 mL HC

4.6 x 50mm HC4.6 x 100mm HC10 x 100mm HC

0.45 mL4.6 x 50mm HC4.6 x 100mm HC10 x 100mm HC

0.45 mL

DimensionMARS Human Albumin

deplete AlbuminMARS Human Albumin / lgG

deplete Albumin, lgG

MARS Mouse 3Albumin, lgG, Transferrin

MARS Mouse 6Albumin, lgG, lgA, Transferrin, Haptoglobin,

Antitrypsin

MARS Mouse 7Albumin, lgG, lgA, Transferrin, Haptoglobin,

Antitrypsin

MARS Mouse 147 + α2-macroglobμLin, α1-acidglycoprotein, IgM,

ApolipoproteinAlet All, ComplementC3, Transthyretin

65 μL130 μL50 μL100 μL

37-60 μL75-100 μL25-30 μL15-20 μL30-40 μL30-40 μL60-80 μL

300-325 μL7-10 μL14-16 μL30-35 μL60-70 μL

250-300 μL12-14 μL

20 μL40 μL250 μL10 μL

Dimension




MicroArrays and NGS

MicroArraysSlides and reagents→ Sensibility : Best of the market : -60mers→ Flexibility Wide range of catalogue chips Custom chip : fully customizable content→ Multi-Applications DNA and RNA CGH, CGH-SNP Epigenetic (Methylation, ChIP-on-Chip, miRNA) Transcriptome (mRNA, lincRNA)

MicroArrays platform: SureScan Scanner

→ High Resolution Scanner : 2μm→ Scan time : 2μm, 2 colors simultaneously in 24 min→ Dynamic autofocus, autoloader→ Best linear dynamic range on the market: 20 bit / scan→ Integrated barcode reader→ Integrated Ozone filter

Integrated bioinformatics solutions

→ eArray/SureDesign→ Feature Extraction→ CytoGenomics→ GeneSpring



New Generation SequencingSureSelect QXT: Libraries preparation without mechanical fragmentation in one day with one hybridization

Haloplex High-Sensitivity: Detection of variants poorly represented in sample (<1%)

→ Complete target enrichment and NGS banks preparation→ Multiplatform solution compatible with most sequencers on the market→ FAST: 1 day to prepare your library→ FFPE tissues; 96 indexes available→ Flexible and customizable : wide range of catalogue kits (exome, genes panel, …)Introns, exons, UTR’s, multi-species, multi-applications : RNAseq, Methylseq, DNAseq

→ Full sequences enrichment→ SNPs Analysis, translocations, deletions, CNVs→ Flexible and customizableExome, Clinical Exome, Focused Exome, Inherited Disease Panel, Cancer Panel, …

SureSelect

→ Easy: thousands of fragments in ONE tube→ Quick Protocol < 6h→ Enzymatic fragmentation→ High efficiency in repeated regions→ 8 existing panels: Exome, Cancer, Cardiomyopathy, LAM, …

Haloplex

→ Free software of NGS data analysis→ Entry before or after alignment

SureCall→ Free and quick software Custom design in 10 min→ UCSC Genome Browser Visualization

SureDesign

Bioinformatics solutions




Instruments

→ Accuracy, speed and versatility→ Intuitive software including HRM analysis→ Hot Top, touch-screen, no PC connection required→ Open system and fully customizable: from 1 to 6 Dyes with Plug & Play system

AriaMx Real-Time PCR System

Description SpecificationExcitation source 8 dye specific LEDs per optical module

Detection 8 silicon photodiodes per optical module

Dyes (at launch) SYBR/FAM, HEX, ROX, CY3, CY5, Atto 425 6 slots, swappable cartridges, no reference channel needed

Chemistries supported SYBR, Probes, HRM

Thermal system Peltier-based, 96 well

Description Specification

Thermal system temperature range

25.0 - 99.9 ºCFAST: Heating: 6ºC/secCooling: 2.5ºC/secAccuracy: ±0.2°C or better of target

Cycling speeds 40 cycles in 43 minutes

Warranty & Reliability

Two year warrantyOn-board QC testing systemLong version: 125 pain pointsShort version: 65 pain points

Sample containers 0.2 mL tubes, 96 well plates, strip tubes

Samples Analysis



Samples Analysis

→ Gradient, Fast→ Interchangeable blocks: 96 and 384 wells→ Touch-screen, Multi-Users→ Wizard Function: Automatic program generation based on Tm and sequence without gradient→ Back-Up > 10,000 storable programs→ Incubator function

SureCycler 8800 System - Gradient Thermocycler

AutomationBRAVO Liquid Handling System→ Pipetting Automate for 96, 384 or 1536 microplates→ Easy to use and program→ Compact system: can be installed in a laminar flow hood→ Gripper option for handling microplates→ Volume range: from 300nL to 250μL→ Many protocols for NGS, CGH, standardization, PCR set-up, …→ More than 60 accessories to configure applications→ Control by software VWorks

PLATELOC→ Automatic sealing for any plates type: microplates, deepwell…→ Gas purging option→ Use in stand-alone or integrated into an automated system→ Compact, robust and reliable→ Great seal compatibility: peelable, pierceable, aluminium…→ Operation via a touch-screen

OffGel 3100→ Isoelectric Point (pHi) separation→ Reproducible and sensitive→ InGel or OffGel→ Resolution up to pH 0,1




Samples Quality-Control

→ Two analysis principles: electrophoresis and flow cytometry→ Sample Volume for nucleic acids: 1μL→ Sample Volume for proteins: 4μL→ Analysis of 12 samples in 30 to 40 min→ RNA quality-control through RIN algorithm→ Reproducibility of experiments→ Digital data to facilitate analysis, archiving and storage→ Kits ready and easy to use→ Referenced in more than 10.000 publications→ Compendium of Applications on www.genomics.agilent.com/Bionalyzer

BioAnalyzer 2100 SystemQualitative and quantitative Multi-applications PlatformDNA, RNA, proteins and cells

→ Flexibility of use and reproducibility→ Sample Volume: 1-2 μL→ Quality-Control analysis in more high-throughput level→ Analysis of 2 to 16 samples on ONE screentape→ RNA quality-control through RINe algorithm→ gDNA quality-control through DIN algorithm DNA Integrity Number→ Kits ready and easy to use

TapeStation 2200 SystemQualitative and quantitative Multi-applications PlatformDNA, gDNA, RNA, and proteins



→ Flexibility of use and reproducibility→ Complete high-throughput automation for true end-to-end sample processing→ Analysis of 1 to 96 samples at constant cost per sample→ Ready-to-use ScreenTape consumables enable easy switching between assays→ FAST: 1 to 2 min for ONE sample < 90 min for 96 samples→ Sample Volume: 1-2 μL → Full range of sizing applications for DNA and RNA→ New kits available: D5000 et D50000 High-Sensitivity

TapeStation 4200 System - Complete Solution for Complete SuccessQualitative and quantitative Multi-applications PlatformDNA, gDNA, ARNs with DIN and RINe algorithms

DNA RNA

Genomic DNA200 to > 60kbp 1 μL of sample

D100035 to 1000bp

1 μL of sample

HS D100035 to 1000bp

2 μL of sample

D5000100 to 5000bp 1 μL of sample

HS D5000100 to 5000bp 2 μL of sample

RNA100 to 6000bp 1 μL of sample

HS RNA100 to 6000bp 2 μL of sample

10 - 100ng/μL 0.1 - 50ng/μL 10 - 1000pg/μL 0.1 - 50ng/μL 10 - 1000pg/μL 25 - 500ng/μL 500 - 10,000ng/μLLOD

0.5 ng/μLLOD

0.1 ng/μLLOD

5 pg/μLLOD

5 pg/μLLOD

0.1 ng/μLLOD

5 ng/μLLOD

100 pg/μLDNA

Integrity Number

RNA Integrity Number equivalent




56

Contact us

United-Kingdom / IrelandEmailToll Free Number

[email protected] / [email protected]

0845 712 5292 (UK) / 01605 8324 (IE)

Austria / SwitzerlandEmailToll Free Number

[email protected]

01 25125 495 (AT) / 08 4880 3560 (CH)

Germany

Toll Free [email protected]

0800 603 1000

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Italy


800 012 575 (opt. 1)

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0034 901 11 68 90 (opt. 3)

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Toll Free [email protected] / [email protected]

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