Microscope BasicsMicroscope Basics

Parts and focusingParts and focusing

Label the Compound Light Label the Compound Light MicroscopeMicroscope((HandoutHandout))

Ocular lensOcular lens

Body TubeBody TubeCoarse Adjustment KnobCoarse Adjustment KnobFine adjustment KnobFine adjustment KnobRevolving nosepieceRevolving nosepieceObjective lenses (4x, 10x, 40x)Objective lenses (4x, 10x, 40x)ArmArmStage Stage Stage ClipsStage ClipsDiaphramDiaphramLight sourceLight source

BaseBase

Focusing with a MicroscopeFocusing with a Microscope Plug in and turn on.Plug in and turn on.

Lower stage completely.Lower stage completely.

Turn nosepiece to low objective.Turn nosepiece to low objective.

Raise stage with coarse focus knob until specimen Raise stage with coarse focus knob until specimen is seen. (Big knob)is seen. (Big knob) Look from the side.Look from the side.

Fine focus the specimen. (Smaller knob)Fine focus the specimen. (Smaller knob)

Centre specimen in the field of view.Centre specimen in the field of view.

Rotate nosepiece to medium power Rotate nosepiece to medium power objective. objective. Look from the side.Look from the side.

Refocus with fine adjustment.Refocus with fine adjustment.

Re-centre and focus using the fine Re-centre and focus using the fine adjustment.adjustment.

Adjust the diaphragm. (Light)Adjust the diaphragm. (Light)

Start over to look at another specimen.Start over to look at another specimen.

Carrying and storage instructionsCarrying and storage instructions. .

Microscope and Cell Theory Microscope and Cell Theory Assignment – pages 141Assignment – pages 141

Questions 1 & 4 in green boxQuestions 1 & 4 in green box