Science 10Microscopes

and CellsName:_________________________________

May 2010

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Notes: The Microscope………………………………………………………………………………………………….………………………………………………………………………………………………….………………………………………………………………………………………………….………………………………………………………………………………………………….………………………………………………………………………………………………….

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Practice: The Microscope

1. Define the parts of the microscope. (pg 478)

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Microscope Part FunctionEyepiece or Ocular

Coarse adjustment knob

Fine adjustment knob

Revolving nosepiece

Objective lenses

Stage

Stage clips

Diaphragm

Lamp or Mirror

Arm

Base

2. How is the electron microscope different from the light microscope?

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3. What is the function of the diaphragm?

4. Where is the ocular lens found?

Notes: The Microscope……………………………………………………………………………………………………………………………………………………………………….…………………………..………………………………………………………………………………………………………………………………………………………………….…………………………………………….………………………………………………………….……………………………………………………………….………………………………………………………….

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Practice: Using the Microscope and How to Draw

1. Convert 150 μm into mm.

2. Convert 0.034mm into μm.

3. What is the magnification of an object that is viewed with a 60X ocular lens and a 150X objective lens?

4. If an object takes up ¼ of the field of view under medium power what is the actual size of the object?

5. If an object is 0.025mm without magnification what is the magnification of a drawing that is 13cm?

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6. If the field of view for the diagram below is 9 cm at 10x magnification, what is the size of this object at 40x magnification?

7.If eight cells fit across the field of view on a microscope at high power (400x) determine the size of the object with no magnification in um.

MAKING A BIOLOGICAL DIAGRAM

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All diagrams must follow the following rules:1. Use unlined, white paper.2. Draw only one cell (one image) - and draw only what you can see!! (Some slides may have several cells showing, pick only one and draw it!!)3. Draw the image approximately half a page in size using clear, unbroken lines.4. Add details to your diagram using only the OUTLINE of the structure. ***Do NOT shade or colour your diagrams!! Stains used in slide preparation alter

the natural colour.5. Labels are drawn using straight horizontal lines and written in lower case (no

capital letters) to the right of the diagram!!6. Measure, with a ruler, and record on the diagram the length of the diagram in

millimetres.7. In the lower right hand corner of your diagram include:

Your nameSubject of drawingPower Magnification of microscopeSize of the object.

8. On back page of drawing include the following information:Power Magnification of microscopeF. O. V.Fit numberCalc. size of object

Practice: Making a Biological Diagram

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How to Determine the Magnification of a Microscope Activity

When looking into a compound microscope, you are actually looking through two lenses.

Your Name: _______________

Subject of Drawing:_______________

Magnification Power: _____________

Size of Object: ________________

Field of View ________________

Fit Number ________________

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a) the lens in the eyepiece (ocular lens)b) the objective lens in the nose piece

In our school microscopes, the magnification of the lens in the eyepiece is _________________.

The school microscope has three different objective lenses.a) low power = _________________b) medium power = _________________c) high power = ________________

To calculate the magnification of your microscope, multiply the ocular (eyepiece) magnification by the objective magnification.

eyepiece magnification * objective magnification = total magnification On our school microscope, the total magnification for:

a) Low power is _________ * ________ =_____________X

b) Medium power is ________* ________=_____ ______ X

c) High power is ____________* ____________= _______ X

**** On scientific drawings, you MUST write the magnification that you used in the lower hand corner of the page.****

UNDERSTANDING THE FIELD OF VIEW

The field of view (F.O.V.) is the area that can be seen when looking through the lens of a microscope.

Place a ruler on the stage of the microscope. Starting with the lowest power, measure, by looking through the lens and counting marks on the ruler, the field of view of your microscope on each setting.

a) F.O.V. of microscope on low power ______________ mmb) F.O.V. of microscope on medium power ___________ mmc) F.O.V. of microscope on high power ___________ mmOn high power were you able to measure the F.O.V.?________ Why or why not?

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Power Ocular Objective Magnification F.O.V. Lens Lens

Low

Medium

High

Did the field of view increase or decrease as the magnification increased?

Would the F.O.V. increase or decrease if you decrease the magnification of your microscope?

The F.O.V. at high power can be calculated by using the following procedure:

a) record the F.O.V. at low power _____________ mm

b) record the magnification at low power ________________ X

c) record the magnification at high power ______________ X

d) Divide high magnification power by low magnification power. This gives you the amount the magnification increased.

e) The magnification increased by __________ times

*** If magnification increased by a certain number times the F.O.V. decreased by the same number.

Thus the F.O.V. at high power is _____________ (use calculations).

Summary Chart:

Notes: The Cell

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Practice: Parts of the Cell

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Letter Name of organelle

Description of function

A

B

C

D

E

F

G

H

Letter Name of organelle

Description of function

A

B

C

D

E

F

G