microfluidic technologies for sample preparation discover the presentation held by benjamin roussel...
TRANSCRIPT
November© 2015
From Technologies to Market
Microfluidic technologies for
Sample Preparation
LOAC ASIA 2015
Benjamin ROUSSEL
From Technologies to Market
Image: courtesy of Bio-Rad
2
PRESENTATION OUTLOOK
15 + 5 mins
• Yole Développement Introduction
• What is sample preparation ?
• Current bottlenecks and challenges
• Microfluidic for Sample Preparation
• Q&A
4
MEMS &
Sensors
LED
Compound
Semi.
Imaging Photonics
MedTech
Manufacturing
Advanced
PackagingPV
Power
Electronics
FIELDS OF EXPERTISE
Yole Développement’s 30 analysts operate in the following areas
©2015 | www.yole.fr | Medical Electronics Symposium 2015
5
OUR GLOBAL ACTIVITY
©2015 | www.yole.fr | Medical Electronics Symposium 2015
6
YOLE ACTIVITES
©2015 | www.yole.fr | Medical Electronics Symposium 2015
© 2015
Microfluidics for Sample Prep
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
8
SAMPLE PREPARATION IN IN-VITRO DIAGNOSTICS
What is sample preparation?
Introduction
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample
CollectionDetection
Whole Blood
Serum
Plasma
Urine
Swab
Food sample
Crop Water
Electrochemical
detection
Optical detection
Acoustic detection
Sample nature
Type of detection
SAMPLE PREPARATION
9
HOW TO DEFINE NECESSARY SAMPLE PREP STEPS?
General steps
A common path among all different types
of assays
Sample
preparation
main
objectives
Sample collection
and stabilization1
Extraction of the
analyte of interest
(e.g. cell lysis)
2
Removal of the
interferences and inhibitors,
isolation and protection of
the analyte
« Sample purification »
3
Labelling
5Detection
6
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
4
Adaptation of the
sample properties (pH,
concentration…) and/or
amplification (e.g. PCR)
10
HOW TO DEFINE NECESSARY SAMPLE PREP STEPS?
Defining the appropriate workflow
SP Workflow = combination of
functions
Mixing
Stirring
Heating
Dilution
SedimentationConcentration
Shearing
Separation
Fusion
Amplification
Cooling
WashingPrecipi
-tation
pH adjustment
Elution
Labeling
Degassing
Extraction Cell Lysis
Dissolution
Homogenizati
on
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SAMPLE PREPARATION IN IN-VITRO DIAGNOSTICS
Often mandatory, always challenging
Sample preparation is challenging
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Time
consuming
Important
hands-on time
Equipment 1
Equipment I1
Equipment I1I
Equipment IV
Multi
Equipment
Often
expensing
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WHY MICROFLUIDICS IS OF INTEREST FOR SAMPLE PREP
Automation is key
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Microfluidics allows
AUTOMATION
Avoid manipulation Enable use by untrained people
Reduce or suppress hands-on timeReduce the amounts of reagents
Reduce environment exposureReduce risk of contamination
Reduce variability Increase reproducibility
13
MICROFLUIDICS FIND APPLICATIONS IN DIFFERENT FIELD
Field of applications
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample prep
for Molecular Diagnostics
(Including Digital PCR)
Sample prep for
Single Cell
Sample prep for Next Generation
Sequencing
Sample prep for immunoassays
Focus of today’s presentations
© 2015
Market perspective
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
15
MICROFLUIDICS HISTORY
Sample prepis now verycritical
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample collection
• Solid
• Swab
• Water solution
• Blood
• …
Sample Injection
• Interface Micro-Macro
• Connectors
• Air bubbles
• …
Sample preparation
• Mixing (reagents)
• purification, filtration
• Concentration
• Lysis
• …
Amplification
• PCR
• qPCR
Detection
• Optical
• Fluorescence
• Electro-chemical
• …
Most Microfluidic developments
have focused on detection
phase
Lack of technologies
for sample preparation step
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A MAJOR MILESTONE FOR THE MICROFLUIDICS INDUSTRY
Market adoption
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
TIME
1990 2000 2015 2020?
Adoption of microfluidic technologies
for Sample prep
Adoption of microfluidics
technologies for detection
17
NGS EXAMPLE
NGS
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample collection
• Solid
• Swab
• Water solution
• Blood
• …
Sample Injection
• Interface Micro-Macro
• Connectors
• Air bubbles
• …
Sample preparation
• Mixing (reagents)
• purification, filtration
• Concentration
• Lysis
• …
Amplification
• PCR
• qPCR
Detection
• Optical
• Fluorescence
• Electro-chemical
• …
2015 2000’s
18
THE 2015-2016 TRANSITION IS AN INFLECTION POINT!
Sample prep market
breakdown
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
The growth of microfluidics for single cell will slow down in 2017, meanwhile the
microfluidics for genomics sample preparation market will continue an impressive growth
19
FIRST LEVEL PACKAGE MICROFLUIDIC DEVICE MARKET FOR SP
Total Accessible Market without SP for Immunoassays
It’s just the beggining
©2015 | www.yole.fr | Final report
0
50
100
150
200
250
300
350
400
2014 2015 2016 2017 2018 2019 2020
Microfluidics for Sample Prep Market ($M)
Microfluidics for Molecular
Diagnostics Sample
Preparation
Microfluidics for Genomics
Sample Preparation
Microfluidics for Single Cell
20
SINGLE CELL SAMPLE PREP
Through microfluidics technologies
Single cell
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample prep for
Single Cell
Some of the key drivers:
- Throughput
- Hands-on time
- Temperature control
- Shear stress reduction
21
NGS SAMPLE PREP
Through microfluidics technologies
Next generation sequencing
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample prep for
NGS
Some of the key drivers:
- Throughput
- Flexibility
- Type of sample accepted
22
CONCLUSIONS
• During years, microfluidics technologies have focused on the detection phase solving thebottlenecks in term of specificity, sensitivity, portability…
• Today, the main remaining challenges for IVD companies remained at the samplepreparation levels. To automate this complex workflow microfluidics technology makesense !
• The adoption of microfluidics solutions for sample preparation as already started inmolecular diagnostics, single cells and NGS assays.
• Sample prep problems solving through microfluidics devices will boost the microfluidictechnology exponentially by offering sample to answer results
Summary
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
23
THANKYOU FOR YOUR ATTENTION
Visit ourtable top
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Time for Q&A
Our new report is now
available for sales
Take a look at our websiteswww.yole.fr
andwww.i-micronews.com
25
MEMS &
Sensors
LED
Compound
Semi.
Imaging Photonics
MedTech
Manufacturing
Advanced
PackagingPV
Power
Electronics
FIELDS OF EXPERTISE
Yole Développement’s 30 analysts operate in the following areas
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
26
4 BUSINESS MODELS
o Consulting and Analysis• Market data & research, marketing analysis
• Technology analysis
• Strategy consulting
• Reverse engineering & costing
• Patent analysis
www.yole.fr
o Reports• Market & Technology reports
• Patent Investigation and patent infringement risk analysis
• Teardowns & Reverse Costing Analysis
• Cost Simulation Tool
www.i-Micronews.com/reports
o Financial services• M&A (buying and selling)
• Due diligence
• Fundraising
• Maturation of companies
• IP portfolio management & optimization
www.yolefinance.com
www.bmorpho.com
o Media• i-Micronews.com website
• @Micronews e-newsletter
• Technology magazines
• Communication & webcast services
• Events
www.i-Micronews.com
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27
A GROUP OF COMPANIES
Market,
technology and
strategy
consulting
www.yole.fr
M&A operations
Due diligences
www.yolefinance.com
Fundraising
Maturation of companies
IP portfolio management & optimization
www.bmorpho.com
Manufacturing costs analysis
Teardown and reverse engineering
Cost simulation tools
www.systemplus.fr
IP analysis
Patent assessment
www.knowmade.fr
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
28
OUR GLOBAL ACTIVITY
Yole JapanYole Inc.
Yole
Korea
40% of our business is in
EU countries30% of our business is in
North America
30% of our business is in
Asia
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
29
SERVING THE ENTIRE SUPPLY CHAIN
Our analysts provide market analysis,
technology evaluation,
and business plan along the entire supply
chain
Integrators and
end-users
Device
makers
Suppliers: material,
equipment, OSAT,
foundries…
Financial investors,
R&D centers
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
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CONTACT INFORMATION
o Consulting and Specific Analysis
• North America: Steve LaFerriere
Email: [email protected]
• Japan: Yutaka Katano, General Manager, Yole Japan & President, Yole K.K.
Email: [email protected]
• RoW: Jean-Christophe Eloy, President & CEO, Yole Développement
Email: [email protected]
o Report business
• North America: Steve LaFerriere
Email: [email protected]
• Europe: Fayçal El Khamassi, Headquarter Sales Coordination & Customer Service
Email: [email protected]
• Japan & Asia: Takashi Onozawa, Sales Asia & General Manager, Yole K.K.
Email: [email protected]
• Korea: Hailey Yang, Business Development Manager, Korean Office
Email: [email protected]
o Financial services
• Jean-Christophe Eloy, CEO & President
Email: [email protected]
o General
• Email: [email protected]
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