Microbubble Generation for Medical Applications

Shirley Zhang

Faculty Mentor: Abraham LeeGraduate Student: Kanaka Hettiarachchi

Overview

Microfluidic devices for generating bubbles – test for optimal conditions

Create double layer bubbles to contain lipid and oil surrounding gas

Test stability and attachment of bubbles in vitro

Making Devices

U.V.

Silicon wafer

PhotoresistMask

PDMS

Microfluidic channels

Develop

Monolayer bubbles

Components: Lipid

(DSPE/DPPE-PEG2000)

Gas (PFC or N2)

Diameter: ~15 um

ColorCam10x magnification

Fluorescence

DiI mixed into lipids

ColorCam40x magnification

Double Layer Bubbles

Components: Lipid Oil Gas (PFC or N2)

No lipid shell – no concentrated fluorescence around bubbles

FastCam

Problems

DSPC/DSPE-PEG2KTriacetin

N2

FastCam

Future Direction

Successfully make stable double layer bubbles

Microbubbles as a drug delivery system in cell culture