Microbubble Generation for Medical Applications
Shirley Zhang
Faculty Mentor: Abraham LeeGraduate Student: Kanaka Hettiarachchi
Overview
Microfluidic devices for generating bubbles – test for optimal conditions
Create double layer bubbles to contain lipid and oil surrounding gas
Test stability and attachment of bubbles in vitro
Making Devices
U.V.
Silicon wafer
PhotoresistMask
PDMS
Microfluidic channels
Develop
Monolayer bubbles
Components: Lipid
(DSPE/DPPE-PEG2000)
Gas (PFC or N2)
Diameter: ~15 um
ColorCam10x magnification
Fluorescence
DiI mixed into lipids
ColorCam40x magnification
Double Layer Bubbles
Components: Lipid Oil Gas (PFC or N2)
No lipid shell – no concentrated fluorescence around bubbles
FastCam
Problems
DSPC/DSPE-PEG2KTriacetin
N2
FastCam
Future Direction
Successfully make stable double layer bubbles
Microbubbles as a drug delivery system in cell culture
