5/23/2018 Melanin Assay Protocol

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Melanin assay

* 1 Day

1. 24well Melan-a cell(500) 24 incubatin (5!104cell"#well)

* 2 Day

2. D$%& wa"'in "uctin

+. , (n/+) &ale 500 3 1 &ale 3

4. 24 incubatin

* + Day

5. D$%& wa"'in "uctin

. , (n/+) &ale 500 3 2 &ale 3

6. 47 incubatin

* 5 Day7. D$%& wa"'in "uctin

8. 19 9a:; 120 ?@ lateA BC(EFG) +0H 55IC incubatin

11. JJK wellC 100L C 8well tNan"OeN

12. P well 9a:; 100

5/23/2018 Melanin Assay Protocol

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