Medical School Histology Basics Epithelium and junctions

VIBS 289 lab

Larry Johnson Texas A&M University

Epithelium - function• Epithelium forms the outer protective surface of the

body and all the glands. It lines the walls of most closed cavities of the body and all passages leading to the exterior, and it lines blood and lymphatic vessels.

skin gland kidney cavities bladder vessels

Epithelium - function Epithelial tissues participate in the metabolism of the body through: i) the absorption of substances from the exterior, e.g., from the lumen of the intestines; and ii) the elimination of other substances to the outside. All substances, including gases, normally received and given off from the body must pass through epithelia. For the performance of secretory functions, the epithelial tissues form specialized structures called glands.

Intestine Sweatgland

Origin and Distribution of Epithelium

Ectoderm - epidermis of skin and epithelium of cornea together covers the entire surface of the body; sebaceous and mammary glands

Endoderm - alimentary tract, liver, pancreas, gastric

glands, intestinal glands• Endocrine glands - lose

connection with surface

Mesoderm• Endothelium - lining of blood vessels• Mesothelium - lining serous cavities

ECTODERM

ENDODERM

MESODERM

CLASSIFICATION OF EPITHELIA

NUMBER OF LAYERS– SIMPLE - ONE LAYER– STRATIFIED - MORE THAN ONE LAYER

SHAPE OF SUPERFICIAL CELLS– SQUAMOUS - FLAT– CUBOIDAL - CUBE– COLUMNAR – COLUMNOTHER– PSEUDOSTRATIFIED - NOT TRULY

STRATIFIED– TRANSITIONAL - URINARY TRACT

Slide 109: Skin, hand, monkey

Simple squamous endothelial cells lining blood lymph vessels– Stratified squamous epithelium of skin– Stratified cuboidal of sweat ducts

Stratified cuboidal

dermis

dermis

epidermisSweat gland

32409 Rat intestine Smooth muscle

Small intestinal villi

Simple columnar epithelium

Goblet cells

148

EM 3: region of basal lamina and connective tissue beneath intestinal epithelial absorptive cells

1. Plasma membrane

2. Basal lamina

3. Connective tissueGoblet cell

Intestinal absorptivecell nuclei

Brush border

32409EM 3

EM 3: Intestine(Basal part of cell)

Basal lamina

Intestinal absorptivecellsConnective

tissue

148

Slide 258: Kidney (PAS)

Basement membrane of epitheliumSimple squamous epithelium

Simple cuboidal in medulla and cortex

Ileum148

Simple columnar epithelium

Intestinal absorptive cells and goblet cells

Goblet cells

Brush border

Ileum, monkey (PAS)249

Basement membrane of epitheliumSimple columnar epithelium

Goblet cells

Brush border

Connective tissue

Cell nuclei

Basement membrane, brush border and goblet cells are PAS positive for sugars

EM 10f

The basal lamina of epithelial cells usually is thin (EM 10f); however, under pathological conditions, it is thickened as in the lymphatic vessel and seminiferous epithelium (Sertoli cell) of an aged-rat testis (EM 8h).

EM 8h

basal lamina

SPECIALIZATION OF EPITHELIA

MAINTAIN EXTENSIVE CONTACTS AMONG CELLS

STRUCTURALLY AND FUNCTIONALLY POLARIZED

JUNCTIONSZONULA OCCLUDENS - TIGHT JUNCTION (BELT)ZONULA ADHERENS –

ADHERING BELTDESMOSOME (MACULA

ADHERENS) - SPOT ATTACHMENTGAP JUNCTIONS - COMMUNICATION

Terminal bars

32409

148 250

250

32409

Terminal bars

Terminal bars are part of a belt (zonula)around the cells.

belt

Intestinal absorptive cells

Goblet cells

EM 4: Intestine (Apical)

Tight junction, zonula adherens,

terminal web

148

Goblet cells

EM 2: Liver

– Gap junction,– desmosome,

tight junction

EM 2a: Liver- Gap junctions

EM 2

133133

250

Simple squamous epithelium cellsviewed on their sides

vein

Lumen of lymphatic vessel

valve

valvecapillary

Simple squamous epithelium cellsviewed “face on”

venule

Simple squamous epithelium

Lumen of lymphatic vessels

arteriole

valve109

19680testis

196

valve

vein

capillary

EM 10A showing capillary endothelial cells109

Simple squamous epithelium : cellsviewed on the side

Stratified squamous epithelium 109

Keratinized dead flattered cells at surface

Slide 109: Skin, hand, monkeyprickle cell" layer (desmosomes)

dermis

epidermis

Slide 109 : Skin, hand, monkey

Stratified cuboidal is only found in sweat ducts of skin (Slide 109).

Fat cells

epidermisdermis

HISTO052 tongue Vagina (Slide 178)

non-keratinized stratified squamous epithelium

Nuclei present in flat cells at surface

Slide 133: Trachea, monkey

– Simple squamous lining the air spaces of lungs

– Pseudostratified epithelium lining tracheal lumen

– Ciliated epithelium of trachea, goblet cells, thick basement membrane

Lungair space

432

133

tracheal lumen (Slide 133),

transition of the luminal epithelium from stratified squamous of vocal cords to pseudostratified, ciliated epithelium

human larynx, Slide 429

stratified squamous

Note thickened basement membrane typical of this epithelium

pseudostratified, ciliated epitheliumcalled respiratory epithelium

Plasma cells133

Transitional epithelium urinary bladder (Slide 160) ureter (Slide 262)

Slide 160 Image of the Urinary bladder

Transitional epithelium

Mesothelium

Lumen of bladder

HISTO052 (tongue) to observe mucus and serous glands

In summary

All substances, including gases, normally received and given off from the body must pass through epithelia.

Which junction - description pair match?a. Zonula occludes – tight junction around the cellb. Zonula adherens – adhering junction around the cellc. Hemidesmosome – spot attachment of cells to the CT belowd. a & be. a, b, & c

Which item(s) is/are characteristic of epithelia?a. secretory cells of glandsb. covers organsc. line urinary tractd. a & be. a, b, & c

Questions on Epithelia

Which embryonic origin - distribution of epithelium do not match? a. endoderm - endothelium b. endoderm - alimentary tract c. mesoderm - mesothelium d. ectoderm - mammary gland e. ectoderm - epidermis

• Bruce Alberts, et al. 1983. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY.• Bruce Alberts, et al. 1994. Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY.

• William J. Banks, 1981. Applied Veterinary Histology. Williams and Wilkins, Los Angeles, CA.

• Hans Elias, et al. 1978. Histology and Human Microanatomy. John Wiley and Sons, New York, NY.

• Don W. Fawcett. 1986. Bloom and Fawcett. A textbook of histology. W. B. Saunders Company, Philadelphia, PA.

• Don W. Fawcett. 1994. Bloom and Fawcett. A textbook of histology. Chapman and Hall, New York, NY.

• Arthur W. Ham and David H. Cormack. 1979. Histology. J. S. Lippincott Company, Philadelphia, PA.

• Luis C. Junqueira, et al. 1983. Basic Histology. Lange Medical Publications, Los Altos, CA.• L. Carlos Junqueira, et al. 1995. Basic Histology. Appleton and Lange, Norwalk, CT.

• L.L. Langley, et al. 1974. Dynamic Anatomy and Physiology. McGraw-Hill Book Company, New York, NY.

• W.W. Tuttle and Byron A. Schottelius. 1969. Textbook of Physiology. The C. V. Mosby Company, St. Louis, MO.

• Leon Weiss. 1977. Histology Cell and Tissue Biology. Elsevier Biomedical, New York, NY.• Leon Weiss and Roy O. Greep. 1977. Histology. McGraw-Hill Book Company, New York, NY.

• Nature (http://www.nature.com), Vol. 414:88,2001.• Arthur C. Guyton,1971.Textbook of Medical Physiology W.B. Saunders company, Philadelphia, PA• WW Tuttle and BA Schottelius 1969 Textbook of Physiology C.V. Mosby Co.

• A.L. Mescher 2013 Junqueira’s Basis Histology text and atlas, 13th ed. McGraw

Many illustrations in these VIBS Histology YouTube videos were modified from the following books and sources: Many thanks to original sources!

Ranch just south of Fort Stockton, TX

On road from Fort Stockton to Big Bend National Park, TX

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