mechanism of action of enzymes- by hurnaum karishma (student ssr medical college)
DESCRIPTION
Mechanism of action of enzymesTRANSCRIPT
![Page 1: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/1.jpg)
Selected for Publication in Student’s corner
Biochemistry for Medics
www.namrata.co
![Page 2: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/2.jpg)
Mechanism of action of enzymes
Presented by: Hurnaum karishma Roll number 32
![Page 3: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/3.jpg)
Table of contents• Introduction of enzymes
• Active site
• Thermodynamic changes
• Covalent catalysis
• Acid base catalysis
• Catalysis by bond strain
• Catalysis by proximity and orientation
![Page 4: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/4.jpg)
• Biological catalysts
• Neither consumed nor permanently altered • All enzymes are proteins in nature except ribozymes
which are RNA in nature
• Highly efficient
• Act as selective catalysts
Introduction of enzymes
![Page 5: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/5.jpg)
Site where actual reaction occurs
Substrate –bound by weak interaction
Specificity of enzyme depend on arrangement
of atom in active site
Active site
![Page 6: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/6.jpg)
1
• Thermodynamic changes
2• Processes at the active site
Mechanism of action of enzyme
The catalytic efficiency of enzyme is explained by 2 perspectives:
![Page 7: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/7.jpg)
Thermodynamic changes
![Page 8: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/8.jpg)
Substrate products
• acquire a transitional state.
• The difference in energy level of transitional state and substrate is called activational barrier.
![Page 9: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/9.jpg)
![Page 10: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/10.jpg)
![Page 11: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/11.jpg)
Only a few substrate can cross this barrier to be converted to products.
That is why rate of uncatalysed reaction is much slow.
When enzyme is present it provides an alternative pathway for conversion of substrate into products.
![Page 12: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/12.jpg)
Enzymes accelerate reaction rate by providing transition states with low activational energy for formation of products
Hence reaction rate is enhanced by many folds in the presence of enzymes
The total energy of the system remains the same and equilibrium state is not disturbed
![Page 13: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/13.jpg)
![Page 14: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/14.jpg)
Processes at the active site
![Page 15: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/15.jpg)
Pro
cess
es
at
act
ive s
ite
Covalent catalysis
Acid base catalysis
Catalysis by bond strain
Catalysis by proximity & orientation
![Page 16: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/16.jpg)
• Enzyme form covalent linkages with substrate forming transient enz-subs complex with very low activational barrier.
• Enzyme is then released unchanged and unconsumed and substrate is converted into products.
Covalent catalysis
![Page 17: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/17.jpg)
![Page 18: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/18.jpg)
• This process is mostly undertaken by transferases and hydrolases.
• The hydrolytic enzyme especially proteolytic enzyme works in this manner for cleavage of peptide bonds in proteins.
• Proteolytic enzymes mostly have serine at their active site so OH goup of serine makes a transient complex with COOH group of peptide bond with subsequent cleavage by water.
![Page 19: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/19.jpg)
![Page 20: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/20.jpg)
Acid base catalysis
• Mostly undertaken by oxido-reductases.
• Mostly at the active site, either histidine is present which act both as a proton donor and a proton acceptor.
• Sometimes aspartic acid, glutamic acid and cysteine residues are also present which participate in Hydrogen transfer reaction.
![Page 21: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/21.jpg)
pyruvate
NAD+ NADH + H+
LactateLDH
![Page 22: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/22.jpg)
![Page 23: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/23.jpg)
Catalysis by bond strain
• Mostly undertaken by lyases.
• The enz-subs binding causes reorientation of the structure of substrate in the site due to being in a strain condition.
• Thus transitional state is readily acquired and enzyme maintains that transitional state where the bond is in the unfavourable state and is eventually broken.
• So enzyme induces a strain in the bond which is required to be broken.
![Page 24: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/24.jpg)
![Page 25: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/25.jpg)
Catalysis by proximity and orientation
• This mechanism is mostly undertaken by ligases.
• The rate of reaction is ↑ by bringing substrate closer to each other at the a.site.
• A region of high substrate conc is produced at the a.site.
• The substrate molecule is placed at bond forming distances.
![Page 26: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/26.jpg)
![Page 27: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/27.jpg)
• Since substrate is placed at optimal distances.
• The probability of collision ↑ and substrate is eventually converted into products.
• This mechanism involve the condensation of substrate molecule.
![Page 28: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/28.jpg)
![Page 29: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/29.jpg)
References :-
• Class notes• Biochemistry for
medics• Internet• Harper’s
![Page 30: Mechanism of action of enzymes- By Hurnaum Karishma (Student SSR Medical College)](https://reader038.vdocuments.site/reader038/viewer/2022102901/554974eab4c90595518b5727/html5/thumbnails/30.jpg)
Thank u!