Masaryk University

Enantis, s.r.o.

VOP-026 Šternberk, s.p., division VTÚO Brno

CPN, s.r.o.

E N Z Y M A T I C D E C O N T A M I N A T I O N

O F S U L F U R M U S T A R D

W a r f a r e a g e n t s u l f u r m u s t a r d

used for the first time in 1917 near Belgium city Ypres

easily penetrates to various materials

makes burn marks and blisters due to skin contacts

military use banned by international convention

easy to prepare from common chemicals

T e c h n o l o g y d e v e l o p m e n t

Czech Republic – development of enzymatic decontamination mixture

for sulfur mustard prioritized by NATO

research projects funded by Ministry of Defence 2004 – 2006 and

Ministry of Education 2005 – 2011

degradation of sulfur mustard by enzymes dehalogenases demonstrated

protection of intellectual properties – Czech patent CZ 298287

technology transfer to practice – biotechnology spin-off Enantis

H a l o a l k a n e d e h a l o g e n a s e s

H a l o a l k a n e d e h a l o g e n a s e s

T o x i c s u l f u r m u s t a r d

S

Cl

Cl

ClO

Cll

N o n t o x i c a n a l o g u e

C o m p u t e r m o d e l l i n g

C o m p u t e r m o d e l l i n g

C o m p u t e r m o d e l l i n g

bis(2-chloroethyl) ethersulfur mustard

C o m p u t e r m o d e l l i n g

E x p e r i m e n t a l t e s t s

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0 5 10 15 20

Time (min)

Su

lfu

r m

ust

ard

co

nc.

(m

M)

Spontaneous degradation

Enzyme catalysed hydrolysis

Exponenciální (Spontaneousdegradation)Exponenciální (Enzymecatalysed hydrolysis)

E x p e r i m e n t a l t e s t s

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0 5 10 15 20

Time (min)

Su

lfu

r m

ust

ard

co

nc.

(m

M)

Spontaneous degradation

Enzyme catalysed hydrolysis

Exponenciální (Spontaneousdegradation)Exponenciální (Enzymecatalysed hydrolysis)

P a t e n t - p r o t e c t e d c o n c e p t

R e p o r t s i n s c i e n t i f i c j o u r n a l s

T e c h n o l o g y t r a n s f e r t o p r a c t i s e

1. development of decontamination mixture

2. optimisation of protein production

3. large-scale industrial production

4. product commercialization

5. development of new and improved products

P r o d u c t d e v e l o p m e n t a n d t e s t i n g

testing of dehalogenases in decontamination aerosols

(Pacific Northwest National Laboratory – USA)

testing of dehalogenases in polymer particles

(Foster & Miller, US Army – USA)

testing of dehalogenases in microemulsions

(Defence Science and Technology Laboratory – Great Britain)

testing of dehalogenases in skin decontamination cream

(Bundeswehr Institute of toxicology and pharmacy – Germany)

NATO testing of enzyme decontamination on aircraft interriers

C o n s o r t i u m o f p a r t n e r s

Masaryk University and Enantis, s.r.o.

14 years of research on dehalogenases

> 40 genes, >100 protein variants

know-how from 29 research stages

modern laboratories and facilities for protein engineering

E N Z Y M E D I S C O V E R Y A N D O P T I M I Z A T I O N

isolation and construction of new enzymes

genetic breeding of enzymes with improved catalytic properties

C o n s o r t i u m o f p a r t n e r s

Masaryk University and Enantis, s.r.o.

VOP-026 Šternberk, s.p., division VTÚO Brno

applied research and development of decontaminations and defence against warfare

of mass destruction

personal and infrastruture based on 50 years of experience

T E S T I N G O F E N Z Y M E S W I T H W A R F A R E A G E N T S

study of warfare chemicals with extreme toxic properties

compatibility testing of individual components for decontamination mixtures

C o n s o r t i u m o f p a r t n e r s

Masaryk University and Enantis, s.r.o.

VOP-026 Šternberk, s.p., division VTÚO Brno

CPN, s.r.o.

Production of pharmaceuticals and cosmetics

large-scale industrial fermentations and down-stream processing

P R O C E S S S C A L E – U P A N D P R O D U C T I O N

optimization of enzyme production, pilot-scale and full production

development of protective cream for skin decontamination

E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r

high-quality basic research:

systematic and selective financial support

investment into human resources

competitive infrastructure

financial support and consulting for protection of intellectual properties

business consulting and administrative support

collaboration with local industrial partners

E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r

high-quality basic research:

systematic and selective financial support

investment into human resources

competitive infrastructure

financial support and consulting for protection of intellectual properties

business consulting and administrative support

collaboration with local industrial partners

E x p e r i e n c e s w i t h t e c h no l o g y t r a n s f e r

high-quality basic research:

systematic and selective financial support

investment into human resources

competitive infrastructure

financial support and consulting for protection of intellectual properties

business consulting and administrative support

collaboration with local industrial partners

A c k n o w l e d g e m e n t s

CTT

Jan Josef Loschmidt

(1821 – 1895)

E x c u r s i o n t o l a b o r a t o r i e s

established 1994, funded by industry, 1 laboratory

until today 18 grants, 6 international

laboratories 5, instrumentation 1 mil EUR

in parallel 12 research projects

know-how from 29 research stages, 11 visitors

courses Bioinformatics and Biocatalysis

E x c u r s i o n t o l a b o r a t o r i e s

E x c u r s i o n t o l a b o r a t o r i e s

3 doctors, 10 doctoral students, 5 diploma students, 1 technician