Geotrichum Fusobacterium Lodderomyces Anaerococcus Colletotrichum Edwardsiella Comamonas Pigmentiphaga Moellerella Nesterenkonia Listonella Udeniomyces Erysipelothrix Escherichia Streptomyces Bartonella Hafnia Terrimonas Pseudoxanthomonas Corynebacterium Streptococcus Aerococcus Saccharothrix Facklamia Schizosaccharomyces Tetragenococcus Lechevalieria Clavibacter Shimwellia Brachybacterium Leclercia Providencia Trabulsiella Xanthobacter Emericella Gardnerella Sporothrix Leuconostoc Pseudoclavibacter Alkalibacillus Debaryomyces Turicella Roseomonas Ruminococcus Scedosporium Dysgonomonas Staphylococcus Peptostreptococcus Paenibacillus Balneatrix Solibacillus Prototheca Cupriavidus Geobacillus Aspergillus Arthrobacter Mesorhizobium Acholeplasma Filobasidium Propioniferax Azohydromonas Chromobacterium Curtobacterium Kloeckera Austwickia Hyphomicrobium Cryptococcus Rummeliibacillus Budvicia Aquincola Enterobacter Sporobolomyces Brevundimonas Capnocytophaga Tatlockia Neisseria Salinivibrio Pullulanibacillus Arcanobacterium Tissierella Eggerthella Methanomonas Mucor Mobiluncus Caulobacter Helcococcus Psychrobacillus Campylobacter Blastomonas Wohlfahrtiimonas Thermoactinomyces Herminiimonas Tsukamurella Mycobacterium Bordetella Pichia Vibrio Iodobacter Tenacibaculum Listeria Plesiomonas Haloarcula Shewanella Paecilomyces Thauera Viridibacillus Yokenella Malassezia Novosphingobium Ornithobacterium Epidermophyton Oligella Paracoccus Aureobasidium Eubacterium Dietzia Salimicrobium Klebsiella Mycoplasma Variovorax Samsonia Schizophyllum Scopulariopsis Odoribacter Anaerotruncus Abiotrophia Burkholderia Sodalis Empedobacter Sphingopyxis Lactococcus Sphingobium Microsporum Peptoniphilus Vagococcus Beauveria Morganella Pasteurella Cedecea Bifidobacterium Micrococcus Propionimicrobium Starkeya Prevotella Histophilus Sphingomonas Acetobacter Francisella Photobacterium Propionibacterium Aneurinibacillus Arthrographis Aromatoleum Pediococcus Phoma Xenorhabdus Methylobacillus Fusarium Wolinella Bacteroides Zygosaccharomyces Cellulosimicrobium Helicobacter Rhizobium Terrabacter Ralstonia Butyricimonas Microsporum Castellaniella Borrelia Microbacterium Rheinheimera Wautersiella Saccharopolyspora Rahnella Nocardioides Gluconobacter Sphingobacterium Mannheimia Cohnella Aggregatibacter Cronobacter Lecythophora Riemerella Chaetomium Atopobium Rhizopus Acidovorax Rothia Kytococcus Chryseobacterium Alishewanella Gemella Methylobacterium Haemophilus Adlercreutzia Buttiauxella Weeksella Alloiococcus Bacillus Arxiozyma Halococcus Rhodotorula Pseudochrobactrum Leminorella Candidatus Xanthomonas Pectobacterium Brevibacterium Arthroderma Slackia Trueperella Inquilinus Brevibacillus Brachyspira Porphyromonas Aurantimonas Actinomyces Eikenella Kitasatospora Magnusiomyces Psychrobacter Acidiphilium Amycolatopsis Lactobacillus Marinibacillus Megamonas Dermatophilus Grimontia Acinetobacter Lysinibacillus Hanseniaspora Parvimonas Moesziomyces Legionella Aliivibrio Dermacoccus Exiguobacterium Virgibacillus Raoultella Gordonia Dialister Parabacteroides Cardiobacterium Stenotrophomonas Sporobolomyces Coprobacillus Sporosarcina Brenneria Rathayibacter Arsenophonus Penicillium Pseudomonas Rubrivivax Bilophila Alloscardovia Nocardia Halomonas Rhodococcus Bergeyella Malikia Actinocorallia Aeromonas Micromonospora Alcaligenes Alistipes Pannonibacter Dickeya Kocuria Ochrobactrum Agrococcus Gracilibacillus Chromohalobacter Yersinia Oerskovia Gallibacterium Erwinia Agromyces Filifactor Devosia Pragia Massilia Collinsella Finegoldia Phenylobacterium Methyloarcula Jonesia Pantoea Elizabethkingia Leifsonia Pseudozyma Streptosporangium Macrococcus Veillonella Sporolactobacillus Moraxella Clostridium Pandoraea Flavobacterium Halobacterium Taylorella Delftia Sinomonas Carnobacterium Myroides Exophiala Sporopachydermia Nocardiopsis Avibacterium Blautia Salmonella Weissella Herbaspirillum Ideonella Kingella Kluyvera Enterococcus Janthinobacterium Kerstersia Luteibacter Photorhabdus Proteus Arcobacter Actinobaculum Alternaria Citrobacter Dichelobacter Achromobacter Candida Ewingella Trichophyton Granulicatella Leptothrix Suttonella Dermabacter Hydrogenophaga Cellulomonas Azoarcus Trichosporon Tatumella Rhodospiridium Acidaminococcus Actinobacillus Serratia Halomonas Rhodococcus Bergeyella Malikia Actinocorallia Aeromonas Micromonospora Alcaligenes Alistipes Pannonibacter Dickeya Kocuria Ochrobactrum Agrococcus Gracilibacillus Chromohalobacter Yersinia Oerskovia Gallibacterium Erwinia Agromyces Filifactor Devosia Pragia Massilia Collinsella Finegoldia Phenylobacterium Methyloarcula Jonesia Pantoea Elizabethkingia Leifsonia Pseudozyma Streptosporangium Macrococcus Veillonella Sporolactobacillus Moraxella Clostridium Pandoraea Flavobacterium Halobacterium Taylorella Delftia Sinomonas Carnobacterium Myroides Exophiala Sporopachydermia Nocardiopsis Avibacterium Blautia Salmonella Weissella Herbaspirillum Ideonella Kingella Kluyvera Enterococcus Janthinobacterium Kerstersia Luteibacter Photorhabdus Proteus Arcobacter Actinobaculum Alternaria Citrobacter Dichelobacter Achromobacter Candida Ewingella Trichophyton Granulicatella Leptothrix Suttonella Dermabacter Hydrogenophaga Cellulomonas Azoarcus Trichosporon Tatumella Rhodospiridium Acidaminococcus Actinobacillus Serratia Sphingobacterium Mannheimia Cohnella Aggregatibacter Cronobacter Lecythophora Riemerella Chaetomium

Innovation with IntegrityMALDI-TOF

Changing Microbiology

MALDI Biotyper®

Subtyping Module

Bru

ker

Dal

toni

cs is

con

tinua

lly im

prov

ing

its p

rodu

cts

and

rese

rves

the

rig

ht

to c

hang

e sp

ecifi

catio

ns w

ithou

t no

tice.

© B

DA

L 0

8-2

016,

18

45

393

Fast Microorganism Identification Combined with Instant Resistance Marker Detection

Antibiotic resistant bacteria are on the rise and are a major global public health threat. Effective prevention and control are therefore particularly important to reduce the risk of infections associated with antibiotic-resistant microorganisms.

The MALDI Biotyper MALDI-TOF mass spectrometry system is the fast, accurate and easy-to-use solution for the identification of microorganisms that is revolutionizing the way microbial identifications are performed.

The MALDI Biotyper Subtyping software module enables the detection of specific resistances in an automated workflow which expands the application range of microbial mass spectrometry significantly. The module currently supports Bacteroides fragilis subtyping for carbapenem resistance and Staphylococcus aureus subtyping for MRSA detection. More applications will be added to the module in the future.

Identification results for B. fragilis with indication of cfiA status for the best match

Subtyping B. fragilis result table

B. fragilis is the most frequently isolated anaerobic pathogen. Carbapenem resistance in B. fragilis is frequently associated with presence of the cfiA gene encoding a metallo-beta-lactamase conferring resis-tance to nearly all beta-lactam antibiotics. Infections with B. fragilis cfiA positive strains are therefore difficult to treat.

Prerequisite for the automated subtyping process is suc-cessful identification of the bacterium i.e. the log(score) ID value must be ≥ 2.0. The subtyping module then looks for specific peaks associated with the protein expressed by B. fragilis cfiA positive strains. If present, the best match for the respective sample will be reported as a presumptive cfiA positive strain.

Bacteroides fragilis cfiA Subtyping

Legend: Result report for Staph. aureus identification with indication of the presence of the PSM-mec peptide. Note that there is no indication for the ID with Score value < 2.00.

Staphyloccocus aureus

MRSA Subtyping

MRSA (Methicillin-Resistant Staphylo-coccus aureus) is resistant to beta-lactam antibiotics and especially troublesome in hospitals, nursing homes and other facilities where immunocompromised patients, with open wounds or invasive devices are at great risk of nosocomial infection.

As with B. fragilis, successful identifica-tion of Staph. aureus is the prerequisite for the subsequent resistance subtyping process. For Staph. aureus the module looks for identification of the PSM-mec peak. PSM-mec is a surface peptide and the proportion of MRSA with PSM-mec is variable, depending on regional epide-miology. Absence of this peak does not mean that the respective strain is not an MRSA, but if detected, it is MRSA.

An Early Warning System

The MBT Subtyping Module quickly detects cfiA positive B. fragilis strains and PSM-mec carrying MRSA. Please note that negative results do not necessarily mean that these strains are susceptible but will require additional confirmation methods.

Seamless and Fast Workflow

To acquire a subtyping result, no additional sample preparation is required once the samples are directly transferred to MALDI target plates. Once the sample has been successfully identified, the software automatically performs the resistance subtyping and result reporting.

Transfer sample directly and add matrix*

Final review and validation

* Only the direct transfer method should be used for resistance subtyping. Preparation procedures including extraction / washing steps risk removal of surface bound peptides / proteins.

Select anisolated colony

1 2

Create project and start measurement

3

Automated subtyping processafter high confi-dence identification

5

Spectrum instantly matched against reference library to perform identification

4

6

Order Information

Bru

ker

Dal

toni

cs is

con

tinua

lly im

prov

ing

its p

rodu

cts

and

rese

rves

the

rig

ht

to c

hang

e sp

ecifi

catio

ns w

ithou

t no

tice.

© B

DA

L 0

8-2

016,

18

45

393

Bruker Daltonik GmbH

Bremen · GermanyPhone +49 (0)421-2205-0 Fax +49 (0)421-2205-103

Bruker Daltonics Inc.

Billerica, MA · USA Phone +1 (978) 663-3660 Fax +1 (978) 667-5993

ms.sales.bdal@bruker.com - www.bruker.com

For research use only. Not for use in diagnostic procedures.

MALDI Biotyper is a registered trademark of Bruker Daltonik GmbH in the European Union, Japan and USA.

Part-No. 1842250The MBT Subtyping Module enables the automated detection of strain specific characteristics.

MBT Compass software (Part-No. 1843241) is prerequisite for the use of the MBT Subtyping Module.